pycom07g20260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009529
Physical Location & Seq
Forward (+)
22411778 .. 22413365
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20260.2

Sequence Viewer

Length: 690 bp
ATGGCTGTTTCATTCCACTCGGTGCCGGGCTTCAATTCCACGGCCAAGTACCAGAATGTTTCAAAAGGTTCTGTTGGAGTTTCTATACCAGCGGCCACCACCCAATTCACATCCTTCTTTGGAGCATTTGGACAAAGGAGGAATGTAAGCGAAAAACGATGGGGTTTATGTCTCTCAGTTGCTGATAGCAATCAGCTCACCACAGACACTGGTGACAAGGCTTCTGAAACTACTGCAAGTTCTCCTGATGACCGATTACCACCTGTTGATTCAACATCTTCGCCTCGGTTCCAAACTAGCGTCCTTGATAAGTCCGGATCTCAAACTTCTGACGTTCAACCCAAGTCAAAAAGATCGCCGCTAACAGCAAGGGAGAGATTGAGGGCGGCCAAGGTTCTTAGTCGTTACACAGACTCAAAGCCGTTGAAAAAATCAGCTAGGGGTAAGAATGTATTAGATGCTCTTAGAGACAGCGAGCGTGGGAAGAAAAGGTCCGGACTTCCAGAGGCCCCTACAAACATTTTTGACGACAGTAAGCGAGGAATGCCAAAGCAGGGCCTAACTTGGGATTTTCCGGGGGGCAATGATCTGTTTCTAATTCTCTTTTCATTTGTATTTATCAGCACGGTGATGTTCGCTACAACGTACGTAGTTTGGAAACTTGGTGCCATCCATTTCAATGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

25.04

Weight (kDa)

9.91

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 665
AccIII TCCGGA 2 cut(s) 314, 494
AciI CCGC 3 cut(s) 92, 359, 386
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 3 cut(s) 42, 93, 387
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 2 cut(s) 50, 647
AfiI CCNNNNNNNGG 2 cut(s) 554, 565
AgsI TTSAA 6 cut(s) 34, 63, 273, 338, 427, 679
AluBI AGCT 2 cut(s) 196, 437
AluI AGCT 2 cut(s) 196, 437
Alw26I GTCTC 2 cut(s) 176, 462
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 2 cut(s) 182, 209
Aor13HI TCCGGA 2 cut(s) 314, 494
AoxI GGCC 5 cut(s) 42, 93, 387, 507, 556
AspS9I GGNCC 3 cut(s) 492, 508, 556
AsuC2I CCSGG 2 cut(s) 27, 576
AsuHPI GGTGA 3 cut(s) 190, 224, 640
AvaII GGWCC 1 cut(s) 492
BanI GGYRCC 2 cut(s) 22, 665
BccI CCATC 2 cut(s) 153, 677
BceAI ACGGC 2 cut(s) 57, 406
BcnI CCSGG 2 cut(s) 27, 576
BcoDI GTCTC 2 cut(s) 176, 462
BfaI CTAG 2 cut(s) 297, 438
BisI GCNGC 3 cut(s) 93, 359, 387
BlsI GCNGC 3 cut(s) 94, 360, 388
Bme1390I CCNGG 2 cut(s) 27, 576
Bme18I GGWCC 1 cut(s) 492
BmgT120I GGNCC 3 cut(s) 492, 508, 556
BmiI GGNNCC 4 cut(s) 24, 290, 510, 667
BmrFI CCNGG 2 cut(s) 27, 576
BmsI GCATC 1 cut(s) 448
BpuMI CCSGG 2 cut(s) 27, 576
BsaAI YACGTR 1 cut(s) 649
BsaBI GATNNNNATC 1 cut(s) 189
BsaJI CCNNGG 4 cut(s) 39, 284, 390, 575
BsaWI WCCGGW 2 cut(s) 314, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 565
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 589
Bse8I GATNNNNATC 1 cut(s) 189
BseAI TCCGGA 2 cut(s) 314, 494
BseDI CCNNGG 4 cut(s) 39, 284, 390, 575
BseGI GGATG 2 cut(s) 110, 669
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 554, 565
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 214
BshFI GGCC 5 cut(s) 44, 95, 389, 509, 558
BshNI GGYRCC 2 cut(s) 22, 665
BsiSI CCGG 4 cut(s) 26, 315, 495, 575
BsiWI CGTACG 1 cut(s) 645
BslI CCNNNNNNNGG 2 cut(s) 554, 565
BsmAI GTCTC 2 cut(s) 176, 462
BsmI GAATGC 1 cut(s) 549
BsnI GGCC 5 cut(s) 44, 95, 389, 509, 558
Bsp13I TCCGGA 2 cut(s) 314, 494
Bsp143I GATC 3 cut(s) 317, 353, 586
BspACI CCGC 3 cut(s) 92, 359, 386
BspANI GGCC 5 cut(s) 44, 95, 389, 509, 558
BspCNI CTCAG 1 cut(s) 188
BspEI TCCGGA 2 cut(s) 314, 494
BspLI GGNNCC 4 cut(s) 24, 290, 510, 667
BspPI GGATC 1 cut(s) 325
BspT107I GGYRCC 2 cut(s) 22, 665
BsrDI GCAATG 1 cut(s) 589
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 39, 284, 390, 575
BssMI GATC 3 cut(s) 317, 353, 586
BssT1I CCWWGG 1 cut(s) 390
Bst4CI ACNGT 2 cut(s) 533, 628
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 3 cut(s) 175, 398, 464
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 110, 669
BstKTI GATC 3 cut(s) 320, 356, 589
BstMAI GTCTC 2 cut(s) 176, 462
BstMBI GATC 3 cut(s) 317, 353, 586
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 25, 574
BstSNI TACGTA 1 cut(s) 649
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 5 cut(s) 44, 95, 389, 509, 558
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 110, 669
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 476
CaiI CAGNNNCTG 2 cut(s) 182, 209
Cfr13I GGNCC 3 cut(s) 492, 508, 556
CseI GACGC 1 cut(s) 289
Csp6I GTAC 2 cut(s) 49, 646
CviQI GTAC 2 cut(s) 49, 646
DdeI CTNAG 3 cut(s) 175, 398, 464
DpnI GATC 3 cut(s) 319, 355, 588
DpnII GATC 3 cut(s) 317, 353, 586
DraIII CACNNNGTG 1 cut(s) 22
EaeI YGGCCR 3 cut(s) 42, 93, 387
Eco105I TACGTA 1 cut(s) 649
Eco130I CCWWGG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 492
EcoO109I RGGNCCY 2 cut(s) 508, 556
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaiI YATR 2 cut(s) 86, 169
Fnu4HI GCNGC 3 cut(s) 93, 359, 387
FokI GGATG 2 cut(s) 97, 656
Fsp4HI GCNGC 3 cut(s) 93, 359, 387
FspBI CTAG 2 cut(s) 297, 438
GluI GCNGC 3 cut(s) 93, 359, 387
HaeIII GGCC 5 cut(s) 44, 95, 389, 509, 558
HapII CCGG 4 cut(s) 26, 315, 495, 575
HgaI GACGC 1 cut(s) 289
HinfI GANTC 2 cut(s) 269, 413
HpaII CCGG 4 cut(s) 26, 315, 495, 575
HphI GGTGA 3 cut(s) 190, 224, 640
Hpy188I TCNGA 2 cut(s) 226, 331
Hpy188III TCNNGA 4 cut(s) 245, 315, 495, 503
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 2 cut(s) 533, 628
HpyCH4IV ACGT 3 cut(s) 333, 644, 648
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 175, 398, 464
HpySE526I ACGT 3 cut(s) 333, 644, 648
Kpn2I TCCGGA 2 cut(s) 314, 494
Kzo9I GATC 3 cut(s) 317, 353, 586
LmnI GCTCC 1 cut(s) 122
LweI GCATC 1 cut(s) 448
MaeI CTAG 2 cut(s) 297, 438
MaeII ACGT 3 cut(s) 333, 644, 648
MaeIII GTNAC 2 cut(s) 212, 404
MalI GATC 3 cut(s) 319, 355, 588
MboI GATC 3 cut(s) 317, 353, 586
MboII GAAGA 2 cut(s) 270, 496
MflI RGATCY 1 cut(s) 317
MluCI AATT 4 cut(s) 34, 104, 597, 685
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 5 cut(s) 132, 294, 375, 499, 533
MroI TCCGGA 2 cut(s) 314, 494
MslI CAYNNNNRTG 2 cut(s) 629, 678
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 4 cut(s) 26, 315, 495, 575
MspR9I CCNGG 2 cut(s) 27, 576
Mva1269I GAATGC 1 cut(s) 549
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 2 cut(s) 27, 576
NdeII GATC 3 cut(s) 317, 353, 586
NlaIV GGNNCC 4 cut(s) 24, 290, 510, 667
NmuCI GTSAC 1 cut(s) 212
PctI GAATGC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 269
Pfl23II CGTACG 1 cut(s) 645
PkrI GCNGC 3 cut(s) 94, 360, 388
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 649
PspLI CGTACG 1 cut(s) 645
PspN4I GGNNCC 4 cut(s) 24, 290, 510, 667
PspPI GGNCC 3 cut(s) 492, 508, 556
PstNI CAGNNNCTG 2 cut(s) 182, 209
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 50, 647
RsaNI GTAC 2 cut(s) 49, 646
RseI CAYNNNNRTG 2 cut(s) 629, 678
SatI GCNGC 3 cut(s) 93, 359, 387
Sau3AI GATC 3 cut(s) 317, 353, 586
Sau96I GGNCC 3 cut(s) 492, 508, 556
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 27, 576
SetI ASST 9 cut(s) 70, 198, 265, 336, 396, 439, 494, 647, 651
SfaNI GCATC 1 cut(s) 448
SinI GGWCC 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 629, 678
SnaBI TACGTA 1 cut(s) 649
Sse9I AATT 4 cut(s) 34, 104, 597, 685
SsiI CCGC 3 cut(s) 92, 359, 386
SspMI CTAG 2 cut(s) 297, 438
StyD4I CCNGG 2 cut(s) 25, 574
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 533, 628
TaiI ACGT 3 cut(s) 336, 647, 651
TaqII GACCGA 1 cut(s) 267
TasI AATT 4 cut(s) 34, 104, 597, 685
TauI GCSGC 3 cut(s) 95, 361, 389
TfiI GAWTC 1 cut(s) 269
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 597
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 492
XspI CTAG 2 cut(s) 297, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.