Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007997

Physical Location & Seq

Genomic Coordinates Forward (+)

32114838 .. 32120184

Gene Structure 5347 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1254100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGGTTTGAGGTATATAGCCATCTCCGCTGCATGTACAGCGATAAGCTTTGCAGGCCTCCAGTTGTGGACGGAAACTTCTTTAGTTAAACTAAAATCGGATGGACTGATTGAGGAGAATTTGATTAATGCGGCCAATTTTGGCCATGTATTTGATCTGCTTTTGGGTTCTTATACTACCGTTGGACTGCTGGCAAATTTTGTACTCAATGCATTTATTTTACTCGTTTTATGTCTCAAGACTATATTCTTTGTGGAGTTGTATTCTTCTGAAACTCACAAATTAATCGAACGCCTTATCAATTATGTTATTTACAAGGGCACTTTTCTACCCCTGGTCATTCCTCCTACAATATATCATGCGGGCCTGTGGTCAGTCTGGTTGATTGTTCTTTGTTCTTTAAAGATGTTTCAAGCTTTGGCTCGAGATCGACTTGAACGGTTGAATGCATCCCCTTCTGCCACACCATTGACATATTTTCGTGTGTATTCAGTGTTATTGCTGGTGCTGACTGTTGATATTTTCTGGATAAGGCTGTGTGTGGTGATATATAAAACACTGGGTTTATCTATGTTTCTCTTGTTATTATTTGAGCCTTTCAGTATTGCATTTGAGACATTGCAGGCTATTTTGGTGCATGGATTTCAGTTACTTGATATATGGATCCATCATTCAGCATGGAACAGTTCAAATTGCGAGAGTTCCAAATTATTTGATACATCAGCAGTTGGTTTATTGTTGGAATGGAAAGGCGGTCTTACTCGGAATTTGAGCTTTGTTCTTGATATGGCCACATTGTTAATGGCCCTTGGTCACTACGTGCATATTTGGTGGTTTCATGGCATGGCATTCCATCTGGTGGATGCAATCCTTTTTCTAAACATACGTGCCTTGCTAAGTTCAATTGTAAAACGTATAAAAGGGTTCATCAAACTGAAGAAAGCCTTAGGTGCTCTTCATGCAGCACTTCCAGATGCAACATCTGAAGAACTACGGGCATATGATGATGAATGTGCAATTTGTAGGGAACCTATGGCTAAGGCCAAAAGACTACACTGCAGTCACCTTTTTCATCTTGCATGCTTGAGATCTTGGTTGGATCAAGGTTTGAATGAGATCTATTCATGTCCGACTTGTCGCAAGCCACTTTTTGTAGGTAGACCTGAAAATGAAGTACATCCCCGCAGTGAAGAAACTTCGAGTGATGAGCAGCTTGCTCGTCAAATAAGTTTAGGACTTGGTCGACAAAATACTCCTGGACATGCAATACCTGCTGGTGTGTTTCCCAATCAGACTCAGAACCCTGCGGAAGGCGATCCATGGAGAGGTACCGGACTGGATTCAGATTGGTTGCATACTTGGTCAAGCCAGGGAGTTGACGGGGCAGGTCCTTCTACGGCTGTAGGATCTGTTGGACTGGGGAGAGTTCAGATGATGATGAGGCGTCTTGCATCTGTAGGGGAGACGTATGCGCACACGGCCCTTGAAGATGGTGCGTGGAGCCTCTGGCCTATGAATGCCCCTCAGGTTGCTGCAACTGGTCCACCGATTCCTCCTGCAGACGATGGAAGATACCAAGGAGGAGCACGGAATTTACATATGAGGACCCCCGCACGTACTGTGAATGACAACCTAGCAAACATACTTGCTATGGCTGAGACGGTGAGGGAAGTTCTGCCTCATATGTCAGATGACCTAATTTTCCAGGATTTGCAGCGAACAAATTCAGTTACCGTTACTGTGAATAATCTTCTCCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010363 GO:0010498 GO:0010941 GO:0010942 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031347 GO:0031349 GO:0032446 GO:0034052 GO:0036211 GO:0043067 GO:0043068 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0080134 GO:0080135 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

CUE zf-RING_2

Protein Analysis

588

Amino Acids

65.29

Weight (kDa)

6.57

Isoelectric Point (pI)

38.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_SYVN1_N	PF25563	54 - 315	8.3e-18	E3 ubiquitin-protein ligase synoviolin-like, TPR repeats
zf-RING_2	PF13639	336 - 380	3.1e-08	Ring finger domain
zf-C3HC4	PF00097	338 - 379	1.5e-06	Zinc finger, C3HC4 type (RING finger)
CUE	PF02845	551 - 587	4.8e-06	CUE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007997)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25230 AT4G25230 AT4G25230 AT5G51450 AT5G51450
fragaria_vesca	FvH4_7g27570 FvH4_7g27570 FvH4_7g27570
malus_domestica	MD07G1254100.v1.1
prunus_persica	Prupe.2G277400_v2.0.a1
pyrus_communis	pycom01g19350 pycom07g22680
rosa_chinensis	RchiOBHm_Chr1g0374441
rosa_laevigata	RLG00000026738
rosa_multiflora	Rmu_sc0006877.1_g000003 Rmu_ssc0000007.1_g000009
rosa_roxburghii	Rroxscaffold_4G00283490
rosa_rugosa	Rorug01G0382800 Rorug01G0382900
rosa_samantha	Rh1AG393900 Rh1BG358500 Rh1CG371500 Rh1DG389100
rosa_wichuraiana	Rw1G034220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1473
Acc36I	ACCTGC	2 cut(s)	1279, 1376
Acc65I	GGTACC	1 cut(s)	1328
AccB1I	GGYRCC	1 cut(s)	1328
AccB7I	CCANNNNNTGG	1 cut(s)	859
AccI	GTMKAC	2 cut(s)	1159, 1243
AciI	CCGC	7 cut(s)	27, 131, 362, 753, 1183, 1307, 1611
AclWI	GGATC	5 cut(s)	658, 671, 1107, 1310, 1414
AcoI	YGGCCR	3 cut(s)	132, 142, 789
AcsI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
AcuI	CTGAAG	2 cut(s)	956, 1005
AcyI	GRCGYC	1 cut(s)	1444
AdeI	CACNNNGTG	1 cut(s)	820
AfaI	GTAC	5 cut(s)	37, 204, 1176, 1330, 1618
AfiI	CCNNNNNNNGG	3 cut(s)	859, 1310, 1325
AgsI	TTSAA	7 cut(s)	413, 437, 445, 690, 903, 1111, 1487
AjnI	CCWGG	4 cut(s)	333, 1255, 1368, 1704
AluBI	AGCT	4 cut(s)	48, 416, 774, 1213
AluI	AGCT	4 cut(s)	48, 416, 774, 1213
Alw21I	GWGCWC	2 cut(s)	955, 1588
Alw26I	GTCTC	4 cut(s)	239, 608, 1457, 1652
AlwI	GGATC	5 cut(s)	658, 671, 1107, 1310, 1414
Ama87I	CYCGRG	1 cut(s)	423
AoxI	GGCC	9 cut(s)	55, 132, 142, 364, 789, 804, 1041, 1479, 1508
ApeKI	GCWGC	5 cut(s)	29, 962, 1210, 1532, 1714
ApoI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
AseI	ATTAAT	2 cut(s)	126, 284
Asp700I	GAANNNNTTC	1 cut(s)	1723
Asp718I	GGTACC	1 cut(s)	1328
AspLEI	GCGC	1 cut(s)	1474
AspS9I	GGNCC	6 cut(s)	364, 805, 1388, 1480, 1541, 1605
AsuHPI	GGTGA	3 cut(s)	556, 1055, 1675
AvaI	CYCGRG	1 cut(s)	423
AvaII	GGWCC	3 cut(s)	1388, 1541, 1605
AxyI	CCTNAGG	2 cut(s)	946, 1524
BaeGI	GKGCMC	1 cut(s)	323
BalI	TGGCCA	2 cut(s)	144, 791
BamHI	GGATCC	1 cut(s)	663
BanI	GGYRCC	1 cut(s)	1328
Bbv12I	GWGCWC	2 cut(s)	955, 1588
BbvI	GCAGC	5 cut(s)	16, 974, 1222, 1519, 1726
BccI	CCATC	6 cut(s)	29, 95, 675, 861, 1484, 1559
BceAI	ACGGC	2 cut(s)	1413, 1494
BcgI	CGANNNNNNTGC	2 cut(s)	1199, 1233
BciT130I	CCWGG	4 cut(s)	335, 1257, 1370, 1706
BcoDI	GTCTC	4 cut(s)	239, 608, 1457, 1652
BfaI	CTAG	1 cut(s)	1634
BfmI	CTRYAG	4 cut(s)	1057, 1401, 1455, 1557
BfuAI	ACCTGC	2 cut(s)	1279, 1376
BglII	AGATCT	2 cut(s)	1088, 1116
BisI	GCNGC	6 cut(s)	30, 132, 963, 1211, 1533, 1715
BlsI	GCNGC	6 cut(s)	31, 133, 964, 1212, 1534, 1716
Bme1390I	CCNGG	4 cut(s)	335, 1257, 1370, 1706
Bme18I	GGWCC	3 cut(s)	1388, 1541, 1605
BmeT110I	CYCGRG	1 cut(s)	423
BmgT120I	GGNCC	6 cut(s)	364, 805, 1388, 1480, 1541, 1605
BmiI	GGNNCC	5 cut(s)	665, 1029, 1330, 1502, 1607
BmrFI	CCNGG	4 cut(s)	335, 1257, 1370, 1706
BmrI	ACTGGG	2 cut(s)	569, 1427
BmsI	GCATC	4 cut(s)	458, 853, 964, 1460
BmuI	ACTGGG	2 cut(s)	569, 1427
BpmI	CTGGAG	1 cut(s)	44
Bpu10I	CCTNAGC	1 cut(s)	1038
BpuEI	CTTGAG	2 cut(s)	221, 1105
BsaAI	YACGTR	3 cut(s)	820, 887, 1616
BsaHI	GRCGYC	1 cut(s)	1444
BsaJI	CCNNGG	5 cut(s)	333, 808, 1319, 1369, 1576
BsaWI	WCCGGW	1 cut(s)	1331
Bsc4I	CCNNNNNNNGG	3 cut(s)	859, 1310, 1325
Bse1I	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
Bse21I	CCTNAGG	2 cut(s)	946, 1524
Bse3DI	GCAATG	1 cut(s)	617
BseBI	CCWGG	4 cut(s)	335, 1257, 1370, 1706
BseDI	CCNNGG	5 cut(s)	333, 808, 1319, 1369, 1576
BseGI	GGATG	4 cut(s)	106, 449, 868, 1177
BseLI	CCNNNNNNNGG	3 cut(s)	859, 1310, 1325
BseMI	GCAATG	1 cut(s)	617
BseMII	CTCAG	3 cut(s)	1310, 1538, 1647
BseNI	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
BseRI	GAGGAG	2 cut(s)	128, 1596
BseSI	GKGCMC	1 cut(s)	323
BseXI	GCAGC	5 cut(s)	16, 974, 1222, 1519, 1726
BshFI	GGCC	9 cut(s)	57, 134, 144, 366, 791, 806, 1043, 1481, 1510
BshNI	GGYRCC	1 cut(s)	1328
BsiHKAI	GWGCWC	2 cut(s)	955, 1588
BsiHKCI	CYCGRG	1 cut(s)	423
BsiSI	CCGG	1 cut(s)	1332
BslI	CCNNNNNNNGG	3 cut(s)	859, 1310, 1325
BsmAI	GTCTC	4 cut(s)	239, 608, 1457, 1652
BsmBI	CGTCTC	2 cut(s)	1457, 1652
BsmI	GAATGC	3 cut(s)	451, 848, 1522
BsnI	GGCC	9 cut(s)	57, 134, 144, 366, 791, 806, 1043, 1481, 1510
BsoBI	CYCGRG	1 cut(s)	423
Bsp1286I	GDGCHC	3 cut(s)	323, 955, 1588
Bsp1407I	TGTACA	1 cut(s)	35
Bsp143I	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
Bsp19I	CCATGG	1 cut(s)	1319
BspACI	CCGC	7 cut(s)	27, 131, 362, 753, 1183, 1307, 1611
BspANI	GGCC	9 cut(s)	57, 134, 144, 366, 791, 806, 1043, 1481, 1510
BspCNI	CTCAG	3 cut(s)	1309, 1537, 1648
BspLI	GGNNCC	5 cut(s)	665, 1029, 1330, 1502, 1607
BspMAI	CTGCAG	2 cut(s)	1061, 1561
BspMI	ACCTGC	2 cut(s)	1279, 1376
BspPI	GGATC	5 cut(s)	658, 671, 1107, 1310, 1414
BspQI	GCTCTTC	1 cut(s)	960
BspT107I	GGYRCC	1 cut(s)	1328
BsrDI	GCAATG	1 cut(s)	617
BsrGI	TGTACA	1 cut(s)	35
BsrI	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
BssECI	CCNNGG	5 cut(s)	333, 808, 1319, 1369, 1576
BssMI	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
BssNI	GRCGYC	1 cut(s)	1444
BssT1I	CCWWGG	3 cut(s)	808, 1319, 1576
Bst2UI	CCWGG	4 cut(s)	335, 1257, 1370, 1706
Bst4CI	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
Bst6I	CTCTTC	1 cut(s)	960
BstACI	GRCGYC	1 cut(s)	1444
BstAPI	GCANNNNNTGC	1 cut(s)	1271
BstAUI	TGTACA	1 cut(s)	35
BstBAI	YACGTR	3 cut(s)	820, 887, 1616
BstC8I	GCNNGC	7 cut(s)	55, 192, 364, 624, 1081, 1142, 1215
BstDEI	CTNAG	6 cut(s)	896, 946, 1038, 1296, 1524, 1656
BstDSI	CCRYGG	1 cut(s)	1319
BstENI	CCTNNNNNAGG	1 cut(s)	1308
BstF5I	GGATG	4 cut(s)	106, 449, 868, 1177
BstHHI	GCGC	1 cut(s)	1474
BstKTI	GATC	8 cut(s)	157, 430, 666, 1091, 1102, 1119, 1318, 1409
BstMAI	GTCTC	4 cut(s)	239, 608, 1457, 1652
BstMBI	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
BstMWI	GCNNNNNNNGC	7 cut(s)	26, 38, 54, 950, 959, 1271, 1478
BstNI	CCWGG	4 cut(s)	335, 1257, 1370, 1706
BstNSI	RCATGY	3 cut(s)	36, 1083, 1265
BstSCI	CCNGG	4 cut(s)	333, 1255, 1368, 1704
BstSFI	CTRYAG	4 cut(s)	1057, 1401, 1455, 1557
BstSLI	GKGCMC	1 cut(s)	323
BstV1I	GCAGC	5 cut(s)	16, 974, 1222, 1519, 1726
BstX2I	RGATCY	4 cut(s)	663, 1088, 1116, 1406
BstYI	RGATCY	4 cut(s)	663, 1088, 1116, 1406
Bsu36I	CCTNAGG	2 cut(s)	946, 1524
BsuRI	GGCC	9 cut(s)	57, 134, 144, 366, 791, 806, 1043, 1481, 1510
BtgI	CCRYGG	1 cut(s)	1319
BtsCI	GGATG	4 cut(s)	106, 449, 868, 1177
BtsI	GCAGTG	2 cut(s)	1054, 1192
BtsIMutI	CAGTG	4 cut(s)	498, 557, 1054, 1192
BveI	ACCTGC	2 cut(s)	1279, 1376
Cac8I	GCNNGC	7 cut(s)	55, 192, 364, 624, 1081, 1142, 1215
CfoI	GCGC	1 cut(s)	1474
Cfr13I	GGNCC	6 cut(s)	364, 805, 1388, 1480, 1541, 1605
CseI	GACGC	1 cut(s)	1433
Csp6I	GTAC	5 cut(s)	36, 203, 1175, 1329, 1617
CviQI	GTAC	5 cut(s)	36, 203, 1175, 1329, 1617
DdeI	CTNAG	6 cut(s)	896, 946, 1038, 1296, 1524, 1656
DpnI	GATC	8 cut(s)	156, 429, 665, 1090, 1101, 1118, 1317, 1408
DpnII	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
DraI	TTTAAA	1 cut(s)	402
DraIII	CACNNNGTG	1 cut(s)	820
EaeI	YGGCCR	3 cut(s)	132, 142, 789
Eam1104I	CTCTTC	1 cut(s)	960
EarI	CTCTTC	1 cut(s)	960
Eco130I	CCWWGG	3 cut(s)	808, 1319, 1576
Eco147I	AGGCCT	1 cut(s)	57
Eco47I	GGWCC	3 cut(s)	1388, 1541, 1605
Eco57I	CTGAAG	2 cut(s)	956, 1005
Eco81I	CCTNAGG	2 cut(s)	946, 1524
Eco88I	CYCGRG	1 cut(s)	423
EcoNI	CCTNNNNNAGG	1 cut(s)	1308
EcoO109I	RGGNCCY	2 cut(s)	1388, 1605
EcoRII	CCWGG	4 cut(s)	333, 1255, 1368, 1704
EcoT14I	CCWWGG	3 cut(s)	808, 1319, 1576
EcoT22I	ATGCAT	2 cut(s)	214, 451
ErhI	CCWWGG	3 cut(s)	808, 1319, 1576
Esp3I	CGTCTC	2 cut(s)	1457, 1652
FalI	AAGNNNNNCTT	4 cut(s)	741, 773, 929, 961
FauI	CCCGC	3 cut(s)	355, 1190, 1618
FauNDI	CATATG	3 cut(s)	1000, 1599, 1683
FblI	GTMKAC	2 cut(s)	1159, 1243
Fnu4HI	GCNGC	6 cut(s)	30, 132, 963, 1211, 1533, 1715
FokI	GGATG	4 cut(s)	113, 436, 875, 1164
Fsp4HI	GCNGC	6 cut(s)	30, 132, 963, 1211, 1533, 1715
FspAI	RTGCGCAY	1 cut(s)	1473
FspBI	CTAG	1 cut(s)	1634
FspI	TGCGCA	1 cut(s)	1473
GlaI	GCGC	1 cut(s)	1473
GluI	GCNGC	6 cut(s)	30, 132, 963, 1211, 1533, 1715
GsuI	CTGGAG	1 cut(s)	44
HaeIII	GGCC	9 cut(s)	57, 134, 144, 366, 791, 806, 1043, 1481, 1510
HapII	CCGG	1 cut(s)	1332
HgaI	GACGC	1 cut(s)	1433
HhaI	GCGC	1 cut(s)	1474
Hin1I	GRCGYC	1 cut(s)	1444
Hin6I	GCGC	1 cut(s)	1472
HinP1I	GCGC	1 cut(s)	1472
HincII	GTYRAC	2 cut(s)	1244, 1378
HindII	GTYRAC	2 cut(s)	1244, 1378
HindIII	AAGCTT	2 cut(s)	46, 414
HinfI	GANTC	3 cut(s)	1294, 1340, 1549
HpaII	CCGG	1 cut(s)	1332
HphI	GGTGA	3 cut(s)	556, 1055, 1675
Hpy166II	GTNNAC	5 cut(s)	69, 1160, 1244, 1378, 1544
Hpy188III	TCNNGA	5 cut(s)	238, 425, 526, 782, 971
Hpy8I	GTNNAC	5 cut(s)	69, 1160, 1244, 1378, 1544
HpyAV	CCTTC	3 cut(s)	465, 1304, 1401
HpyCH4III	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
HpyCH4IV	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
HpyF10VI	GCNNNNNNNGC	7 cut(s)	26, 38, 54, 950, 959, 1271, 1478
HpyF3I	CTNAG	6 cut(s)	896, 946, 1038, 1296, 1524, 1656
HpySE526I	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
Hsp92I	GRCGYC	1 cut(s)	1444
HspAI	GCGC	1 cut(s)	1472
KpnI	GGTACC	1 cut(s)	1332
Kzo9I	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
LguI	GCTCTTC	1 cut(s)	960
LmnI	GCTCC	2 cut(s)	1500, 1583
Lsp1109I	GCAGC	5 cut(s)	16, 974, 1222, 1519, 1726
LweI	GCATC	4 cut(s)	458, 853, 964, 1460
MaeI	CTAG	1 cut(s)	1634
MaeII	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
MaeIII	GTNAC	5 cut(s)	648, 812, 1061, 1729, 1735
MalI	GATC	8 cut(s)	156, 429, 665, 1090, 1101, 1118, 1317, 1408
MboI	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
MboII	GAAGA	8 cut(s)	258, 947, 949, 998, 1202, 1499, 1581, 1742
MfeI	CAATTG	1 cut(s)	903
MflI	RGATCY	4 cut(s)	663, 1088, 1116, 1406
MhlI	GDGCHC	3 cut(s)	323, 955, 1588
MlsI	TGGCCA	2 cut(s)	144, 791
MluNI	TGGCCA	2 cut(s)	144, 791
MlyI	GAGTC	1 cut(s)	1288
MmeI	TCCRAC	5 cut(s)	163, 720, 1077, 1154, 1393
Mox20I	TGGCCA	2 cut(s)	144, 791
Mph1103I	ATGCAT	2 cut(s)	214, 451
MroXI	GAANNNNTTC	1 cut(s)	1723
MscI	TGGCCA	2 cut(s)	144, 791
MseI	TTAA	5 cut(s)	87, 126, 284, 401, 800
Msp20I	TGGCCA	2 cut(s)	144, 791
MspA1I	CMGCKG	1 cut(s)	29
MspI	CCGG	1 cut(s)	1332
MspR9I	CCNGG	4 cut(s)	335, 1257, 1370, 1706
MunI	CAATTG	1 cut(s)	903
Mva1269I	GAATGC	3 cut(s)	451, 848, 1522
MvaI	CCWGG	4 cut(s)	335, 1257, 1370, 1706
MwoI	GCNNNNNNNGC	7 cut(s)	26, 38, 54, 950, 959, 1271, 1478
NcoI	CCATGG	1 cut(s)	1319
NdeI	CATATG	3 cut(s)	1000, 1599, 1683
NdeII	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
NlaIV	GGNNCC	5 cut(s)	665, 1029, 1330, 1502, 1607
NmuCI	GTSAC	2 cut(s)	812, 1061
NsbI	TGCGCA	1 cut(s)	1473
NsiI	ATGCAT	2 cut(s)	214, 451
NspI	RCATGY	3 cut(s)	36, 1083, 1265
PaeI	GCATGC	1 cut(s)	1083
PaeR7I	CTCGAG	1 cut(s)	423
PceI	AGGCCT	1 cut(s)	57
PciSI	GCTCTTC	1 cut(s)	960
PctI	GAATGC	3 cut(s)	451, 848, 1522
PdmI	GAANNNNTTC	1 cut(s)	1723
PfeI	GAWTC	2 cut(s)	1340, 1549
PflFI	GACNNNGTC	1 cut(s)	1239
PflMI	CCANNNNNTGG	1 cut(s)	859
PfoI	TCCNGGA	2 cut(s)	1255, 1704
PkrI	GCNGC	6 cut(s)	31, 133, 964, 1212, 1534, 1716
PleI	GAGTC	1 cut(s)	1288
PpsI	GAGTC	1 cut(s)	1288
Ppu21I	YACGTR	3 cut(s)	820, 887, 1616
PpuMI	RGGWCCY	2 cut(s)	1388, 1605
PshBI	ATTAAT	2 cut(s)	126, 284
Psp5II	RGGWCCY	2 cut(s)	1388, 1605
Psp6I	CCWGG	4 cut(s)	333, 1255, 1368, 1704
PspGI	CCWGG	4 cut(s)	333, 1255, 1368, 1704
PspN4I	GGNNCC	5 cut(s)	665, 1029, 1330, 1502, 1607
PspPI	GGNCC	6 cut(s)	364, 805, 1388, 1480, 1541, 1605
PspPPI	RGGWCCY	2 cut(s)	1388, 1605
PstI	CTGCAG	2 cut(s)	1061, 1561
PsuI	RGATCY	4 cut(s)	663, 1088, 1116, 1406
PsyI	GACNNNGTC	1 cut(s)	1239
RsaI	GTAC	5 cut(s)	37, 204, 1176, 1330, 1618
RsaNI	GTAC	5 cut(s)	36, 203, 1175, 1329, 1617
SalI	GTCGAC	1 cut(s)	1242
SapI	GCTCTTC	1 cut(s)	960
SaqAI	TTAA	5 cut(s)	87, 126, 284, 401, 800
SatI	GCNGC	6 cut(s)	30, 132, 963, 1211, 1533, 1715
Sau3AI	GATC	8 cut(s)	154, 427, 663, 1088, 1099, 1116, 1315, 1406
Sau96I	GGNCC	6 cut(s)	364, 805, 1388, 1480, 1541, 1605
SchI	GAGTC	1 cut(s)	1288
ScrFI	CCNGG	4 cut(s)	335, 1257, 1370, 1706
SduI	GDGCHC	3 cut(s)	323, 955, 1588
SfaNI	GCATC	4 cut(s)	458, 853, 964, 1460
SfcI	CTRYAG	4 cut(s)	1057, 1401, 1455, 1557
Sfr274I	CTCGAG	1 cut(s)	423
SinI	GGWCC	3 cut(s)	1388, 1541, 1605
SlaI	CTCGAG	1 cut(s)	423
SmlI	CTYRAG	3 cut(s)	236, 423, 1084
SmoI	CTYRAG	3 cut(s)	236, 423, 1084
SphI	GCATGC	1 cut(s)	1083
SseBI	AGGCCT	1 cut(s)	57
SsiI	CCGC	7 cut(s)	27, 131, 362, 753, 1183, 1307, 1611
SspMI	CTAG	1 cut(s)	1634
StuI	AGGCCT	1 cut(s)	57
StyD4I	CCNGG	4 cut(s)	333, 1255, 1368, 1704
StyI	CCWWGG	3 cut(s)	808, 1319, 1576
TaaI	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
TaiI	ACGT	5 cut(s)	822, 889, 916, 1469, 1618
TaqI	TCGA	5 cut(s)	288, 424, 430, 1199, 1243
TatI	WGTACW	3 cut(s)	35, 202, 1174
TauI	GCSGC	1 cut(s)	134
TfiI	GAWTC	2 cut(s)	1340, 1549
Tru1I	TTAA	5 cut(s)	87, 126, 284, 401, 800
Tru9I	TTAA	5 cut(s)	87, 126, 284, 401, 800
TscAI	CASTG	4 cut(s)	498, 564, 1061, 1192
TseFI	GTSAC	2 cut(s)	812, 1061
TseI	GCWGC	5 cut(s)	29, 962, 1210, 1532, 1714
Tsp45I	GTSAC	2 cut(s)	812, 1061
TspDTI	ATGAA	8 cut(s)	827, 916, 947, 1023, 1061, 1113, 1185, 1529
TspGWI	ACGGA	2 cut(s)	86, 1603
TspRI	CASTG	4 cut(s)	498, 564, 1061, 1192
Tth111I	GACNNNGTC	1 cut(s)	1239
Van91I	CCANNNNNTGG	1 cut(s)	859
VpaK11BI	GGWCC	3 cut(s)	1388, 1541, 1605
VspI	ATTAAT	2 cut(s)	126, 284
XagI	CCTNNNNNAGG	1 cut(s)	1308
XapI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
XceI	RCATGY	3 cut(s)	36, 1083, 1265
XcmI	CCANNNNNNNNNTGG	1 cut(s)	799
XhoI	CTCGAG	1 cut(s)	423
XmiI	GTMKAC	2 cut(s)	1159, 1243
XmnI	GAANNNNTTC	1 cut(s)	1723
XspI	CTAG	1 cut(s)	1634
Zsp2I	ATGCAT	2 cut(s)	214, 451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.