Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0007997

Physical Location & Seq

Genomic Coordinates Forward (+)

18040225 .. 18044100

Gene Structure 3876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g19350.12

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGGTTTGAGATATATAGCCATCTCTGCGGCATGTACAGCAATAAGCTTTGCGGGTCTCCAGTTGTGGACGGAAACTTCTTTAGTTAAACTAAAATCAGATGGACTGATTGCAGAGAATTTGATTAATGCGGCCAATTTTGGCCATGTATTTGATCTGCTTTTGGGTTCTTATACTACCGTTGGACTGCTGGCAAATTTTGTACTCAATGCATTTATTTTACTCGTTCTATGTCTCAAGACTATATTCTTTGTGGAGTTGTATCCTTCTGAAACTCGCAAATTAATTGAACGCCTTGTCAATTATGTTATTTACAAGGGCACTTTTCTACCCCTGGTCATCCCTCCTACAATATATCATGCGGGCATGTGGTCAGTCTGGTTGATTGTTCTTTGTTCTTTAAAGATGTTTCAAGCTTTGGCTCGAGATCGACTTGAACGGTTGAATGCATCCCCTTCTGCCACACCATGGACATATTTTCGTGTGTATTCAGTGTTATTGCTGGTGCTCACCGTTGACATTTTCTGGATAAGGCTGTGTGTGGTGATATATAAAACACTGGGTTTATCCATGTTTCTCCTGTTATTATTTGAGCCTTTCAGTATTGCATTTGAGACACTGCAGGCTATTTTGGTGCATGGATTTCAGTTACTTGATATATGGATCCATCATTCAGCATGGGACAGTTCAAATTGCGAGGGGTCTAAATTATTTGATACATCAGCAGCTGGTTTATTGTTGGAATGGAAAGGCAGTCTTACTCGGAATTTGGGATTTGTTCTTGATATGGCAACATTGTTAATGGCCCTTGGTCACTACGTGCATATTTGGTGGCTTCATGGCATGGCATTCCATCTGGTGGATGCAATCCTTTTTCTAAACATACGTGCCTTGCTAAGTGCAATTTTAAAACGTATAAAGGGGTTCATCAAACTGAGGAAAGCCTTAGGTGCTCTTCATGCAGCACTTCCAGATGCAACATCTGAAGAACTACAAGCATATGATGATGAATGTGCAATTTGTAGGGAACCTATGGCTAAGGCCAAAAGACTACACTGCAGTCACCTTTTTCATCTTGCATGCTTGAGATCTTGGTTGGATCAAGGTTTGAACGAGATATATTCATGTCCGACTTGTCGCAAGCCACTTTTTGTGGGCAGACCTGAAAATGAAATACATCCCCGCAGTGAAGAAACTTCGAATGATGAGCAGCTTGCTCGTCAAATAAGTTCAGGACTTGATCGACAAAATACTCCTGGACATGCAATACCTGCTGGCGTGTTTCCCAATCAGGCTCAGAACCCTTCAGAAGGCGATCCATGGAGGGGTGCAGGACTGGATCCAGATTGGTTGCATACTTGGCCAAGTCAGGGAGTTGACGGGGCAGGTCCTTCTGCAGCCATAGGATCTGTTGGACTGGGGAGAGTTCAGATGATGATGAGGCATCTTGCATCTGTAGGGGAGACGTATGCCCAAACGGCCCTTGAAGATAGTGCGTGGAGCCTCTGGCCTATGAATGCCCCTCAGGTTGCTGCTACTGGTCCATCTATTCCTCCTGCTGACGATGGAAGAAACCATGGAGGAGCACGAAATTTACATACGAGGACCCCCTCACGTACTGTGAATGACAACCTAGCAAACATACTTGCCATGGCTGAGACGGTGAGGGAAGTTCTGCCTCATATGCCAGATGACCTAATTTTCCAGGATTTGCAGCGAACGAATTCAGTTACAGTTACTGTGAATAATCTTCTCCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010363 GO:0010498 GO:0010941 GO:0010942 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031347 GO:0031349 GO:0032446 GO:0034052 GO:0036211 GO:0043067 GO:0043068 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0045088 GO:0045089 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0080134 GO:0080135 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

CUE zf-RING_2

Protein Analysis

588

Amino Acids

65.23

Weight (kDa)

6.37

Isoelectric Point (pI)

37.94

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007997)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25230 AT4G25230 AT4G25230 AT5G51450 AT5G51450
fragaria_vesca	FvH4_7g27570 FvH4_7g27570 FvH4_7g27570
malus_domestica	MD07G1254100.v1.1
prunus_persica	Prupe.2G277400_v2.0.a1
pyrus_communis	pycom01g19350 pycom07g22680
rosa_chinensis	RchiOBHm_Chr1g0374441
rosa_laevigata	RLG00000026738
rosa_multiflora	Rmu_sc0006877.1_g000003 Rmu_ssc0000007.1_g000009
rosa_roxburghii	Rroxscaffold_4G00283490
rosa_rugosa	Rorug01G0382800 Rorug01G0382900
rosa_samantha	Rh1AG393900 Rh1BG358500 Rh1CG371500 Rh1DG389100
rosa_wichuraiana	Rw1G034220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1279, 1376
AccB7I	CCANNNNNTGG	2 cut(s)	468, 859
AciI	CCGC	5 cut(s)	29, 53, 131, 362, 1183
AclWI	GGATC	7 cut(s)	658, 671, 1107, 1310, 1334, 1347, 1414
AcoI	YGGCCR	3 cut(s)	132, 142, 1361
AcsI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
AcuI	CTGAAG	2 cut(s)	1005, 1290
AdeI	CACNNNGTG	1 cut(s)	820
AfaI	GTAC	3 cut(s)	37, 204, 1618
AfiI	CCNNNNNNNGG	5 cut(s)	468, 859, 1310, 1325, 1370
AgsI	TTSAA	7 cut(s)	290, 413, 437, 445, 690, 1111, 1487
AjnI	CCWGG	3 cut(s)	333, 1255, 1704
AluBI	AGCT	4 cut(s)	48, 416, 728, 1213
AluI	AGCT	4 cut(s)	48, 416, 728, 1213
Alw21I	GWGCWC	3 cut(s)	510, 955, 1588
Alw26I	GTCTC	5 cut(s)	62, 239, 608, 1457, 1652
AlwI	GGATC	7 cut(s)	658, 671, 1107, 1310, 1334, 1347, 1414
AlwNI	CAGNNNCTG	2 cut(s)	728, 1739
Ama87I	CYCGRG	1 cut(s)	423
AoxI	GGCC	7 cut(s)	132, 142, 804, 1041, 1361, 1479, 1508
ApeKI	GCWGC	6 cut(s)	725, 962, 1210, 1397, 1532, 1714
ApoI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
AseI	ATTAAT	2 cut(s)	126, 284
Asp700I	GAANNNNTTC	1 cut(s)	1723
AspS9I	GGNCC	5 cut(s)	805, 1388, 1480, 1541, 1605
AsuHPI	GGTGA	4 cut(s)	502, 556, 1055, 1675
AsuII	TTCGAA	1 cut(s)	1199
AvaI	CYCGRG	1 cut(s)	423
AvaII	GGWCC	3 cut(s)	1388, 1541, 1605
AxyI	CCTNAGG	2 cut(s)	946, 1524
BaeGI	GKGCMC	1 cut(s)	323
BalI	TGGCCA	2 cut(s)	144, 1363
BamHI	GGATCC	2 cut(s)	663, 1339
Bbv12I	GWGCWC	3 cut(s)	510, 955, 1588
BbvI	GCAGC	6 cut(s)	737, 974, 1222, 1409, 1519, 1726
BccI	CCATC	6 cut(s)	29, 95, 675, 861, 1552, 1559
BceAI	ACGGC	1 cut(s)	1494
BcgI	CGANNNNNNTGC	2 cut(s)	1199, 1233
BciT130I	CCWGG	3 cut(s)	335, 1257, 1706
BciVI	GTATCC	1 cut(s)	273
BcoDI	GTCTC	5 cut(s)	62, 239, 608, 1457, 1652
BfaI	CTAG	1 cut(s)	1634
BfmI	CTRYAG	4 cut(s)	620, 1057, 1395, 1455
BfuAI	ACCTGC	2 cut(s)	1279, 1376
BfuI	GTATCC	1 cut(s)	273
BglI	GCCNNNNNGGC	1 cut(s)	1478
BglII	AGATCT	1 cut(s)	1088
BisI	GCNGC	8 cut(s)	30, 132, 726, 963, 1211, 1398, 1533, 1715
BlsI	GCNGC	8 cut(s)	31, 133, 727, 964, 1212, 1399, 1534, 1716
Bme1390I	CCNGG	3 cut(s)	335, 1257, 1706
Bme18I	GGWCC	3 cut(s)	1388, 1541, 1605
BmeT110I	CYCGRG	1 cut(s)	423
BmgT120I	GGNCC	5 cut(s)	805, 1388, 1480, 1541, 1605
BmiI	GGNNCC	5 cut(s)	665, 1029, 1341, 1502, 1607
BmrFI	CCNGG	3 cut(s)	335, 1257, 1706
BmrI	ACTGGG	2 cut(s)	569, 1427
BmsI	GCATC	5 cut(s)	458, 853, 964, 1453, 1460
BmuI	ACTGGG	2 cut(s)	569, 1427
BpmI	CTGGAG	1 cut(s)	44
Bpu10I	CCTNAGC	1 cut(s)	1038
Bpu14I	TTCGAA	1 cut(s)	1199
BpuEI	CTTGAG	2 cut(s)	221, 1105
BsaAI	YACGTR	3 cut(s)	820, 887, 1616
BsaI	GGTCTC	1 cut(s)	62
BsaJI	CCNNGG	6 cut(s)	333, 467, 808, 1319, 1576, 1650
Bsc4I	CCNNNNNNNGG	5 cut(s)	468, 859, 1310, 1325, 1370
Bse1I	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
Bse21I	CCTNAGG	2 cut(s)	946, 1524
BseBI	CCWGG	3 cut(s)	335, 1257, 1706
BseDI	CCNNGG	6 cut(s)	333, 467, 808, 1319, 1576, 1650
BseGI	GGATG	4 cut(s)	339, 449, 868, 1177
BseLI	CCNNNNNNNGG	5 cut(s)	468, 859, 1310, 1325, 1370
BseMII	CTCAG	4 cut(s)	926, 1310, 1538, 1647
BseNI	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
BseRI	GAGGAG	1 cut(s)	1596
BseSI	GKGCMC	1 cut(s)	323
BseXI	GCAGC	6 cut(s)	737, 974, 1222, 1409, 1519, 1726
BsgI	GTGCAG	1 cut(s)	1350
BshFI	GGCC	7 cut(s)	134, 144, 806, 1043, 1363, 1481, 1510
BsiHKAI	GWGCWC	3 cut(s)	510, 955, 1588
BsiHKCI	CYCGRG	1 cut(s)	423
BslFI	GGGAC	1 cut(s)	695
BslI	CCNNNNNNNGG	5 cut(s)	468, 859, 1310, 1325, 1370
BsmAI	GTCTC	5 cut(s)	62, 239, 608, 1457, 1652
BsmBI	CGTCTC	2 cut(s)	1457, 1652
BsmFI	GGGAC	1 cut(s)	695
BsmI	GAATGC	3 cut(s)	451, 848, 1522
BsnI	GGCC	7 cut(s)	134, 144, 806, 1043, 1363, 1481, 1510
Bso31I	GGTCTC	1 cut(s)	62
BsoBI	CYCGRG	1 cut(s)	423
Bsp119I	TTCGAA	1 cut(s)	1199
Bsp1286I	GDGCHC	4 cut(s)	323, 510, 955, 1588
Bsp1407I	TGTACA	1 cut(s)	35
Bsp143I	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
Bsp19I	CCATGG	4 cut(s)	467, 1319, 1576, 1650
BspACI	CCGC	5 cut(s)	29, 53, 131, 362, 1183
BspANI	GGCC	7 cut(s)	134, 144, 806, 1043, 1363, 1481, 1510
BspCNI	CTCAG	4 cut(s)	927, 1309, 1537, 1648
BspLI	GGNNCC	5 cut(s)	665, 1029, 1341, 1502, 1607
BspMAI	CTGCAG	3 cut(s)	624, 1061, 1399
BspMI	ACCTGC	2 cut(s)	1279, 1376
BspPI	GGATC	7 cut(s)	658, 671, 1107, 1310, 1334, 1347, 1414
BspQI	GCTCTTC	1 cut(s)	960
BspT104I	TTCGAA	1 cut(s)	1199
BspTNI	GGTCTC	1 cut(s)	62
BsrGI	TGTACA	1 cut(s)	35
BsrI	ACTGG	5 cut(s)	61, 564, 1341, 1422, 1543
BssECI	CCNNGG	6 cut(s)	333, 467, 808, 1319, 1576, 1650
BssMI	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
BssT1I	CCWWGG	5 cut(s)	467, 808, 1319, 1576, 1650
Bst2UI	CCWGG	3 cut(s)	335, 1257, 1706
Bst4CI	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
Bst6I	CTCTTC	1 cut(s)	960
BstAPI	GCANNNNNTGC	1 cut(s)	1271
BstAUI	TGTACA	1 cut(s)	35
BstBAI	YACGTR	3 cut(s)	820, 887, 1616
BstBI	TTCGAA	1 cut(s)	1199
BstC8I	GCNNGC	7 cut(s)	192, 364, 624, 1081, 1142, 1215, 1276
BstDEI	CTNAG	7 cut(s)	896, 935, 946, 1038, 1296, 1524, 1656
BstDSI	CCRYGG	4 cut(s)	467, 1319, 1576, 1650
BstENI	CCTNNNNNAGG	1 cut(s)	1308
BstF5I	GGATG	4 cut(s)	339, 449, 868, 1177
BstKTI	GATC	9 cut(s)	157, 430, 666, 1091, 1102, 1243, 1318, 1342, 1409
BstMAI	GTCTC	5 cut(s)	62, 239, 608, 1457, 1652
BstMBI	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
BstMWI	GCNNNNNNNGC	8 cut(s)	26, 38, 950, 959, 1271, 1360, 1478, 1684
BstNI	CCWGG	3 cut(s)	335, 1257, 1706
BstNSI	RCATGY	4 cut(s)	36, 370, 1083, 1265
BstSCI	CCNGG	3 cut(s)	333, 1255, 1704
BstSFI	CTRYAG	4 cut(s)	620, 1057, 1395, 1455
BstSLI	GKGCMC	1 cut(s)	323
BstV1I	GCAGC	6 cut(s)	737, 974, 1222, 1409, 1519, 1726
BstX2I	RGATCY	4 cut(s)	663, 1088, 1339, 1406
BstYI	RGATCY	4 cut(s)	663, 1088, 1339, 1406
Bsu36I	CCTNAGG	2 cut(s)	946, 1524
BsuI	GTATCC	1 cut(s)	273
BsuRI	GGCC	7 cut(s)	134, 144, 806, 1043, 1363, 1481, 1510
BtgI	CCRYGG	4 cut(s)	467, 1319, 1576, 1650
BtsCI	GGATG	4 cut(s)	339, 449, 868, 1177
BtsI	GCAGTG	3 cut(s)	617, 1054, 1192
BtsIMutI	CAGTG	5 cut(s)	498, 557, 617, 1054, 1192
BveI	ACCTGC	2 cut(s)	1279, 1376
Cac8I	GCNNGC	7 cut(s)	192, 364, 624, 1081, 1142, 1215, 1276
CaiI	CAGNNNCTG	2 cut(s)	728, 1739
Cfr13I	GGNCC	5 cut(s)	805, 1388, 1480, 1541, 1605
Csp6I	GTAC	3 cut(s)	36, 203, 1617
CviQI	GTAC	3 cut(s)	36, 203, 1617
DdeI	CTNAG	7 cut(s)	896, 935, 946, 1038, 1296, 1524, 1656
DpnI	GATC	9 cut(s)	156, 429, 665, 1090, 1101, 1242, 1317, 1341, 1408
DpnII	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
DraI	TTTAAA	2 cut(s)	402, 909
DraIII	CACNNNGTG	1 cut(s)	820
EaeI	YGGCCR	3 cut(s)	132, 142, 1361
Eam1104I	CTCTTC	1 cut(s)	960
EarI	CTCTTC	1 cut(s)	960
Eco130I	CCWWGG	5 cut(s)	467, 808, 1319, 1576, 1650
Eco31I	GGTCTC	1 cut(s)	62
Eco47I	GGWCC	3 cut(s)	1388, 1541, 1605
Eco57I	CTGAAG	2 cut(s)	1005, 1290
Eco81I	CCTNAGG	2 cut(s)	946, 1524
Eco88I	CYCGRG	1 cut(s)	423
EcoNI	CCTNNNNNAGG	1 cut(s)	1308
EcoO109I	RGGNCCY	2 cut(s)	1388, 1605
EcoRI	GAATTC	1 cut(s)	1723
EcoRII	CCWGG	3 cut(s)	333, 1255, 1704
EcoT14I	CCWWGG	5 cut(s)	467, 808, 1319, 1576, 1650
EcoT22I	ATGCAT	2 cut(s)	214, 451
ErhI	CCWWGG	5 cut(s)	467, 808, 1319, 1576, 1650
Esp3I	CGTCTC	2 cut(s)	1457, 1652
FalI	AAGNNNNNCTT	2 cut(s)	741, 773
FaqI	GGGAC	1 cut(s)	695
FauI	CCCGC	3 cut(s)	46, 355, 1190
FauNDI	CATATG	2 cut(s)	1000, 1683
Fnu4HI	GCNGC	8 cut(s)	30, 132, 726, 963, 1211, 1398, 1533, 1715
FokI	GGATG	4 cut(s)	326, 436, 875, 1164
Fsp4HI	GCNGC	8 cut(s)	30, 132, 726, 963, 1211, 1398, 1533, 1715
FspBI	CTAG	1 cut(s)	1634
GluI	GCNGC	8 cut(s)	30, 132, 726, 963, 1211, 1398, 1533, 1715
GsuI	CTGGAG	1 cut(s)	44
HaeIII	GGCC	7 cut(s)	134, 144, 806, 1043, 1363, 1481, 1510
HincII	GTYRAC	2 cut(s)	517, 1378
HindII	GTYRAC	2 cut(s)	517, 1378
HindIII	AAGCTT	2 cut(s)	46, 414
HphI	GGTGA	4 cut(s)	502, 556, 1055, 1675
Hpy166II	GTNNAC	3 cut(s)	69, 517, 1378
Hpy188I	TCNGA	8 cut(s)	100, 271, 765, 985, 1131, 1299, 1309, 1431
Hpy188III	TCNNGA	7 cut(s)	238, 425, 526, 782, 971, 1233, 1343
Hpy8I	GTNNAC	3 cut(s)	69, 517, 1378
HpyAV	CCTTC	5 cut(s)	276, 465, 1304, 1314, 1401
HpyCH4III	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
HpyCH4IV	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
HpyF10VI	GCNNNNNNNGC	8 cut(s)	26, 38, 950, 959, 1271, 1360, 1478, 1684
HpyF3I	CTNAG	7 cut(s)	896, 935, 946, 1038, 1296, 1524, 1656
HpySE526I	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
Kzo9I	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
LguI	GCTCTTC	1 cut(s)	960
LmnI	GCTCC	2 cut(s)	1500, 1583
Lsp1109I	GCAGC	6 cut(s)	737, 974, 1222, 1409, 1519, 1726
LweI	GCATC	5 cut(s)	458, 853, 964, 1453, 1460
MaeI	CTAG	1 cut(s)	1634
MaeII	ACGT	5 cut(s)	819, 886, 913, 1466, 1615
MaeIII	GTNAC	5 cut(s)	648, 812, 1061, 1729, 1735
MalI	GATC	9 cut(s)	156, 429, 665, 1090, 1101, 1242, 1317, 1341, 1408
MboI	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
MboII	GAAGA	6 cut(s)	947, 998, 1202, 1499, 1581, 1742
MflI	RGATCY	4 cut(s)	663, 1088, 1339, 1406
MhlI	GDGCHC	4 cut(s)	323, 510, 955, 1588
MlsI	TGGCCA	2 cut(s)	144, 1363
MluNI	TGGCCA	2 cut(s)	144, 1363
MmeI	TCCRAC	5 cut(s)	163, 720, 1077, 1154, 1393
Mox20I	TGGCCA	2 cut(s)	144, 1363
Mph1103I	ATGCAT	2 cut(s)	214, 451
MroXI	GAANNNNTTC	1 cut(s)	1723
MscI	TGGCCA	2 cut(s)	144, 1363
MseI	TTAA	6 cut(s)	87, 126, 284, 401, 800, 908
Msp20I	TGGCCA	2 cut(s)	144, 1363
MspA1I	CMGCKG	1 cut(s)	728
MspR9I	CCNGG	3 cut(s)	335, 1257, 1706
Mva1269I	GAATGC	3 cut(s)	451, 848, 1522
MvaI	CCWGG	3 cut(s)	335, 1257, 1706
MwoI	GCNNNNNNNGC	8 cut(s)	26, 38, 950, 959, 1271, 1360, 1478, 1684
NcoI	CCATGG	4 cut(s)	467, 1319, 1576, 1650
NdeI	CATATG	2 cut(s)	1000, 1683
NdeII	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
NlaIV	GGNNCC	5 cut(s)	665, 1029, 1341, 1502, 1607
NmuCI	GTSAC	2 cut(s)	812, 1061
NsiI	ATGCAT	2 cut(s)	214, 451
NspI	RCATGY	4 cut(s)	36, 370, 1083, 1265
NspV	TTCGAA	1 cut(s)	1199
PaeI	GCATGC	1 cut(s)	1083
PaeR7I	CTCGAG	1 cut(s)	423
PciSI	GCTCTTC	1 cut(s)	960
PctI	GAATGC	3 cut(s)	451, 848, 1522
PdmI	GAANNNNTTC	1 cut(s)	1723
PflMI	CCANNNNNTGG	2 cut(s)	468, 859
PfoI	TCCNGGA	2 cut(s)	1255, 1704
PkrI	GCNGC	8 cut(s)	31, 133, 727, 964, 1212, 1399, 1534, 1716
Ppu21I	YACGTR	3 cut(s)	820, 887, 1616
PpuMI	RGGWCCY	2 cut(s)	1388, 1605
PshBI	ATTAAT	2 cut(s)	126, 284
Psp5II	RGGWCCY	2 cut(s)	1388, 1605
Psp6I	CCWGG	3 cut(s)	333, 1255, 1704
PspGI	CCWGG	3 cut(s)	333, 1255, 1704
PspN4I	GGNNCC	5 cut(s)	665, 1029, 1341, 1502, 1607
PspPI	GGNCC	5 cut(s)	805, 1388, 1480, 1541, 1605
PspPPI	RGGWCCY	2 cut(s)	1388, 1605
PstI	CTGCAG	3 cut(s)	624, 1061, 1399
PstNI	CAGNNNCTG	2 cut(s)	728, 1739
PsuI	RGATCY	4 cut(s)	663, 1088, 1339, 1406
PvuII	CAGCTG	1 cut(s)	728
RsaI	GTAC	3 cut(s)	37, 204, 1618
RsaNI	GTAC	3 cut(s)	36, 203, 1617
SapI	GCTCTTC	1 cut(s)	960
SaqAI	TTAA	6 cut(s)	87, 126, 284, 401, 800, 908
SatI	GCNGC	8 cut(s)	30, 132, 726, 963, 1211, 1398, 1533, 1715
Sau3AI	GATC	9 cut(s)	154, 427, 663, 1088, 1099, 1240, 1315, 1339, 1406
Sau96I	GGNCC	5 cut(s)	805, 1388, 1480, 1541, 1605
ScrFI	CCNGG	3 cut(s)	335, 1257, 1706
SduI	GDGCHC	4 cut(s)	323, 510, 955, 1588
SfaNI	GCATC	5 cut(s)	458, 853, 964, 1453, 1460
SfcI	CTRYAG	4 cut(s)	620, 1057, 1395, 1455
Sfr274I	CTCGAG	1 cut(s)	423
SfuI	TTCGAA	1 cut(s)	1199
SinI	GGWCC	3 cut(s)	1388, 1541, 1605
SlaI	CTCGAG	1 cut(s)	423
SmlI	CTYRAG	3 cut(s)	236, 423, 1084
SmoI	CTYRAG	3 cut(s)	236, 423, 1084
SphI	GCATGC	1 cut(s)	1083
SsiI	CCGC	5 cut(s)	29, 53, 131, 362, 1183
SspMI	CTAG	1 cut(s)	1634
StyD4I	CCNGG	3 cut(s)	333, 1255, 1704
StyI	CCWWGG	5 cut(s)	467, 808, 1319, 1576, 1650
TaaI	ACNGT	8 cut(s)	181, 441, 514, 686, 1621, 1663, 1735, 1741
TaiI	ACGT	5 cut(s)	822, 889, 916, 1469, 1618
TaqI	TCGA	4 cut(s)	424, 430, 1199, 1243
TatI	WGTACW	2 cut(s)	35, 202
TauI	GCSGC	2 cut(s)	32, 134
Tru1I	TTAA	6 cut(s)	87, 126, 284, 401, 800, 908
Tru9I	TTAA	6 cut(s)	87, 126, 284, 401, 800, 908
TscAI	CASTG	5 cut(s)	498, 564, 624, 1061, 1192
TseFI	GTSAC	2 cut(s)	812, 1061
TseI	GCWGC	6 cut(s)	725, 962, 1210, 1397, 1532, 1714
Tsp45I	GTSAC	2 cut(s)	812, 1061
TspDTI	ATGAA	8 cut(s)	827, 916, 947, 1023, 1061, 1113, 1185, 1529
TspGWI	ACGGA	1 cut(s)	86
TspRI	CASTG	5 cut(s)	498, 564, 624, 1061, 1192
Van91I	CCANNNNNTGG	2 cut(s)	468, 859
VpaK11BI	GGWCC	3 cut(s)	1388, 1541, 1605
VspI	ATTAAT	2 cut(s)	126, 284
XagI	CCTNNNNNAGG	1 cut(s)	1308
XapI	RAATTY	5 cut(s)	118, 196, 766, 1591, 1723
XceI	RCATGY	4 cut(s)	36, 370, 1083, 1265
XhoI	CTCGAG	1 cut(s)	423
XmnI	GAANNNNTTC	1 cut(s)	1723
XspI	CTAG	1 cut(s)	1634
Zsp2I	ATGCAT	2 cut(s)	214, 451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.