MD08G1021600.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017078
Physical Location & Seq
Reverse (-)
1563087 .. 1565432
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1021600.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGATCGTCTCGCCTCCCTCATCCTCCTCCTCTCCCTCCTATCCTCCGCCCTCGCCTCCGCCTCAGGTTCCAACGACGCCGACCCTATCATCCGACAAGTCGTTTCGGAAACTGACGAAGATCATCTCCTCCACGCCAAGCACTTCTCCAGCTTCAAAGCCACGTTCGGGAAGACCTATGCGACTCAGGTTGAGCACGACTACAGGTTCGGCGTCTTCAAGGCCAACCTCCGCCGAGCCAAGCGGCACCAGGCGCTCGACCCCTCTGCCGTCCACGGTGTAACCAAGTTCTCTGATCTCACTCCCAAGGAGTTTCGCAGGAACTTTCTCGGACTAAAGCGTCGTCTCCGGCTTCCGGCCGACGCGAACAATGCTCCGATTCTTCCCACCAATGATCTTCCAACGGACTTTGATTGGCGCGACAAAGGTGCTGTCACGCCGGTGAAGGACCAGGGTTCTTGTGGGTCGTGCTGGGCGTTTAGTGCGACGGGAGCTTTGGAAGGAGCTCATTATTTGGAAACAGGGGAGCTCGTCAGTCTGAGTGAGCAGCAGCTTGTTGACTGTGACCATGAGTGTGATCCGGAAGAATATGGCTCGTGTGACTCGGGTTGCAATGGCGGGCTGATGAACAATGCCTTTGAGTACGCACTCAAGGCTGGCGGGCTAGAACGAGAGGAAGATTATCCTTACACCGGGACTGATGACACCTGCAAATTCGACAAGAGCAAAGTTGTTTCTTCCGTGAATAACTTCAGCGTTATTTCTACCGACGAAGATCAAATCGCTGCAAATTTGGTTAACCATGGCCCTCTTGCAGTTGGGATCAATGCGGTGTTCATGCAAACATACGTAGGCGGAGTTTCGTGCCCTTACATCTGCGGAAAGCGAATTGATCACGGAGTGCTTCTGGTGGGTTATGGTTCTTCGGGTTTTGCTCCGATTCGATTTAAGGAGAAACCGTACTGGATCTTGAAGAATTCATGGGGACAGAGCTGGGGAGAGCAGGGATATTACAAGATCTGCAGGGGTTATAATTCTTGTGGGGTGGATTCCTTGGTCTCAACGGTTGCAGCTCTGCGTACATCCAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04210 map04142 map04210
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

365

Amino Acids

39.84

Weight (kDa)

5.82

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 49 - 105 7.3e-15 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 133 - 355 2.7e-75 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16190
fragaria_vesca FvH4_2g38940
malus_domestica MD08G1021600.v1.1 MD15G1019300.v1.1
prunus_persica Prupe.1G371600_v2.0.a1
pyrus_communis pycom08g01710
rosa_chinensis RchiOBHm_Chr6g0302031
rosa_laevigata RLG00000011201
rosa_roxburghii Rroxscaffold_7G00166170
rosa_rugosa Rorug06G0311100
rosa_samantha Rh6BG478400 Rh6DG424000
rosa_wichuraiana Rw6G036720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
AarI CACCTGC 1 cut(s) 716
AasI GACNNNNNNGTC 1 cut(s) 339
Acc36I ACCTGC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 246
AccII CGCG 2 cut(s) 365, 420
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 9 cut(s) 48, 60, 232, 244, 618, 660, 830, 855, 879
AclWI GGATC 4 cut(s) 12, 572, 830, 974
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 3 cut(s) 713, 790, 976
AcuI CTGAAG 1 cut(s) 736
AcyI GRCGYC 2 cut(s) 78, 213
AdeI CACNNNGTG 1 cut(s) 901
AfaI GTAC 3 cut(s) 644, 962, 1081
AfiI CCNNNNNNNGG 3 cut(s) 168, 355, 692
AgsI TTSAA 3 cut(s) 157, 220, 973
AjnI CCWGG 2 cut(s) 249, 450
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 7 cut(s) 153, 494, 506, 529, 553, 993, 1073
AluI AGCT 7 cut(s) 153, 494, 506, 529, 553, 993, 1073
Alw21I GWGCWC 3 cut(s) 198, 508, 531
Alw26I GTCTC 3 cut(s) 14, 350, 1063
AlwI GGATC 4 cut(s) 12, 572, 830, 974
Ama87I CYCGRG 1 cut(s) 604
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 3 cut(s) 222, 357, 805
ApeKI GCWGC 4 cut(s) 547, 550, 785, 1070
ApoI RAATTY 3 cut(s) 713, 790, 976
ArsI GACNNNNNNTTYG 6 cut(s) 191, 223, 417, 449, 478, 510
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 2 cut(s) 256, 420
AspS9I GGNCC 2 cut(s) 448, 806
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 1 cut(s) 454
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 1 cut(s) 448
AxyI CCTNAGG 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 869
BanI GGYRCC 1 cut(s) 246
BanII GRGCYC 2 cut(s) 508, 531
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 2 cut(s) 179, 208
Bbv12I GWGCWC 3 cut(s) 198, 508, 531
BbvI GCAGC 4 cut(s) 559, 562, 772, 1082
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 410, 444
BciT130I CCWGG 2 cut(s) 251, 452
BclI TGATCA 1 cut(s) 892
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 3 cut(s) 14, 350, 1063
BfaI CTAG 1 cut(s) 665
BfmI CTRYAG 2 cut(s) 202, 1021
BfoI RGCGCY 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 716
BglII AGATCT 1 cut(s) 1017
BisI GCNGC 5 cut(s) 245, 548, 551, 786, 1071
BlsI GCNGC 5 cut(s) 246, 549, 552, 787, 1072
Bme1390I CCNGG 3 cut(s) 251, 452, 694
Bme18I GGWCC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 604
BmgT120I GGNCC 2 cut(s) 448, 806
BmiI GGNNCC 2 cut(s) 70, 248
BmrFI CCNGG 3 cut(s) 251, 452, 694
BpiI GAAGAC 2 cut(s) 179, 208
BpmI CTGGAG 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 694
BsaAI YACGTR 1 cut(s) 850
BsaHI GRCGYC 2 cut(s) 78, 213
BsaI GGTCTC 1 cut(s) 1063
BsaJI CCNNGG 5 cut(s) 274, 306, 451, 802, 1053
BsaWI WCCGGW 1 cut(s) 580
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 355, 692
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 968
Bse21I CCTNAGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 619
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 2 cut(s) 251, 452
BseDI CCNNGG 5 cut(s) 274, 306, 451, 802, 1053
BseGI GGATG 3 cut(s) 21, 90, 1082
BseLI CCNNNNNNNGG 3 cut(s) 168, 355, 692
BseMI GCAATG 1 cut(s) 619
BseMII CTCAG 3 cut(s) 78, 200, 530
BseNI ACTGG 1 cut(s) 968
BseRI GAGGAG 3 cut(s) 17, 20, 119
BseSI GKGCMC 1 cut(s) 869
BseX3I CGGCCG 1 cut(s) 357
BseXI GCAGC 4 cut(s) 559, 562, 772, 1082
BseYI CCCAGC 2 cut(s) 471, 993
Bsh1236I CGCG 2 cut(s) 365, 420
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 224, 359, 807
BshNI GGYRCC 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 3 cut(s) 198, 508, 531
BsiHKCI CYCGRG 1 cut(s) 604
BsiSI CCGG 5 cut(s) 349, 356, 440, 581, 693
BslFI GGGAC 2 cut(s) 709, 999
BslI CCNNNNNNNGG 3 cut(s) 168, 355, 692
BsmAI GTCTC 3 cut(s) 14, 350, 1063
BsmBI CGTCTC 2 cut(s) 14, 350
BsmFI GGGAC 2 cut(s) 709, 999
BsnI GGCC 3 cut(s) 224, 359, 807
Bso31I GGTCTC 1 cut(s) 1063
BsoBI CYCGRG 1 cut(s) 604
Bsp1286I GDGCHC 4 cut(s) 198, 508, 531, 869
Bsp13I TCCGGA 1 cut(s) 580
Bsp19I CCATGG 1 cut(s) 802
BspACI CCGC 9 cut(s) 48, 60, 232, 244, 618, 660, 830, 855, 879
BspANI GGCC 3 cut(s) 224, 359, 807
BspCNI CTCAG 3 cut(s) 77, 199, 531
BspEI TCCGGA 1 cut(s) 580
BspFNI CGCG 2 cut(s) 365, 420
BspLI GGNNCC 2 cut(s) 70, 248
BspMAI CTGCAG 1 cut(s) 1025
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 4 cut(s) 12, 572, 830, 974
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 1063
BsrDI GCAATG 1 cut(s) 619
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 1 cut(s) 968
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 5 cut(s) 274, 306, 451, 802, 1053
BssNI GRCGYC 2 cut(s) 78, 213
BssSI CACGAG 1 cut(s) 595
BssT1I CCWWGG 3 cut(s) 306, 802, 1053
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 2 cut(s) 251, 452
Bst4CI ACNGT 4 cut(s) 278, 563, 960, 1066
BstACI GRCGYC 2 cut(s) 78, 213
BstBAI YACGTR 1 cut(s) 850
BstC8I GCNNGC 3 cut(s) 620, 658, 662
BstDEI CTNAG 3 cut(s) 64, 186, 539
BstDSI CCRYGG 2 cut(s) 274, 802
BstF5I GGATG 3 cut(s) 21, 90, 1082
BstFNI CGCG 2 cut(s) 365, 420
BstH2I RGCGCY 1 cut(s) 257
BstHHI GCGC 2 cut(s) 256, 420
BstMAI GTCTC 3 cut(s) 14, 350, 1063
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 5 cut(s) 253, 371, 482, 491, 653
BstNI CCWGG 2 cut(s) 251, 452
BstSCI CCNGG 3 cut(s) 249, 450, 692
BstSFI CTRYAG 2 cut(s) 202, 1021
BstSLI GKGCMC 1 cut(s) 869
BstSNI TACGTA 1 cut(s) 850
BstUI CGCG 2 cut(s) 365, 420
BstV1I GCAGC 4 cut(s) 559, 562, 772, 1082
BstV2I GAAGAC 2 cut(s) 179, 208
BstX2I RGATCY 2 cut(s) 966, 1017
BstYI RGATCY 2 cut(s) 966, 1017
BstZI CGGCCG 1 cut(s) 357
Bsu36I CCTNAGG 1 cut(s) 64
BsuRI GGCC 3 cut(s) 224, 359, 807
BtgI CCRYGG 2 cut(s) 274, 802
BtsCI GGATG 3 cut(s) 21, 90, 1082
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 3 cut(s) 620, 658, 662
CfoI GCGC 2 cut(s) 256, 420
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 448, 806
CseI GACGC 4 cut(s) 86, 202, 329, 371
Csp6I GTAC 3 cut(s) 643, 961, 1080
CviAII CATG 4 cut(s) 569, 803, 838, 981
CviQI GTAC 3 cut(s) 643, 961, 1080
DdeI CTNAG 3 cut(s) 64, 186, 539
DraIII CACNNNGTG 1 cut(s) 901
DrdI GACNNNNNNGTC 1 cut(s) 339
DseDI GACNNNNNNGTC 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 357
EagI CGGCCG 1 cut(s) 357
EciI GGCGGA 4 cut(s) 37, 49, 221, 870
Ecl136II GAGCTC 2 cut(s) 506, 529
EclXI CGGCCG 1 cut(s) 357
Eco105I TACGTA 1 cut(s) 850
Eco130I CCWWGG 3 cut(s) 306, 802, 1053
Eco24I GRGCYC 2 cut(s) 508, 531
Eco31I GGTCTC 1 cut(s) 1063
Eco47I GGWCC 1 cut(s) 448
Eco52I CGGCCG 1 cut(s) 357
Eco53kI GAGCTC 2 cut(s) 506, 529
Eco57I CTGAAG 1 cut(s) 736
Eco81I CCTNAGG 1 cut(s) 64
Eco88I CYCGRG 1 cut(s) 604
EcoICRI GAGCTC 2 cut(s) 506, 529
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 2 cut(s) 249, 450
EcoT14I CCWWGG 3 cut(s) 306, 802, 1053
EcoT38I GRGCYC 2 cut(s) 508, 531
ErhI CCWWGG 3 cut(s) 306, 802, 1053
Esp3I CGTCTC 2 cut(s) 14, 350
FaeI CATG 4 cut(s) 572, 806, 841, 984
FaiI YATR 9 cut(s) 180, 570, 591, 804, 839, 847, 918, 982, 1032
FaqI GGGAC 2 cut(s) 709, 999
FatI CATG 4 cut(s) 568, 802, 837, 980
FauI CCCGC 2 cut(s) 611, 653
FbaI TGATCA 1 cut(s) 892
Fnu4HI GCNGC 5 cut(s) 245, 548, 551, 786, 1071
FokI GGATG 3 cut(s) 8, 77, 1069
FriOI GRGCYC 2 cut(s) 508, 531
Fsp4HI GCNGC 5 cut(s) 245, 548, 551, 786, 1071
FspBI CTAG 1 cut(s) 665
GlaI GCGC 2 cut(s) 255, 419
GluI GCNGC 5 cut(s) 245, 548, 551, 786, 1071
GsaI CCCAGC 2 cut(s) 475, 997
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 257
HaeIII GGCC 3 cut(s) 224, 359, 807
HapII CCGG 5 cut(s) 349, 356, 440, 581, 693
HgaI GACGC 4 cut(s) 86, 202, 329, 371
HhaI GCGC 2 cut(s) 256, 420
Hin1I GRCGYC 2 cut(s) 78, 213
Hin1II CATG 4 cut(s) 572, 806, 841, 984
Hin6I GCGC 2 cut(s) 254, 418
HinP1I GCGC 2 cut(s) 254, 418
HincII GTYRAC 2 cut(s) 559, 799
HindII GTYRAC 2 cut(s) 559, 799
HinfI GANTC 5 cut(s) 184, 379, 602, 940, 1049
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 5 cut(s) 349, 356, 440, 581, 693
HphI GGTGA 1 cut(s) 454
Hpy166II GTNNAC 3 cut(s) 274, 559, 799
Hpy188I TCNGA 7 cut(s) 95, 109, 295, 332, 378, 540, 939
Hpy188III TCNNGA 3 cut(s) 169, 581, 970
Hpy8I GTNNAC 3 cut(s) 274, 559, 799
Hpy99I CGWCG 5 cut(s) 80, 345, 365, 490, 773
HpyAV CCTTC 2 cut(s) 439, 494
HpyCH4III ACNGT 4 cut(s) 278, 563, 960, 1066
HpyCH4IV ACGT 2 cut(s) 164, 849
HpyCH4V TGCA 7 cut(s) 612, 711, 788, 815, 841, 1023, 1070
HpyF10VI GCNNNNNNNGC 5 cut(s) 253, 371, 482, 491, 653
HpyF3I CTNAG 3 cut(s) 64, 186, 539
HpySE526I ACGT 2 cut(s) 164, 849
Hsp92I GRCGYC 2 cut(s) 78, 213
Hsp92II CATG 4 cut(s) 572, 806, 841, 984
HspAI GCGC 2 cut(s) 254, 418
Kpn2I TCCGGA 1 cut(s) 580
Ksp22I TGATCA 1 cut(s) 892
KspAI GTTAAC 1 cut(s) 799
LmnI GCTCC 5 cut(s) 379, 491, 503, 526, 940
Lsp1109I GCAGC 4 cut(s) 559, 562, 772, 1082
MaeI CTAG 1 cut(s) 665
MaeII ACGT 2 cut(s) 164, 849
MaeIII GTNAC 4 cut(s) 280, 433, 563, 599
MflI RGATCY 2 cut(s) 966, 1017
MhlI GDGCHC 4 cut(s) 198, 508, 531, 869
MluCI AATT 5 cut(s) 713, 790, 888, 976, 1033
MlyI GAGTC 2 cut(s) 178, 596
MmeI TCCRAC 3 cut(s) 96, 118, 425
MroI TCCGGA 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 2 cut(s) 798, 948
MslI CAYNNNNRTG 2 cut(s) 440, 573
MspI CCGG 5 cut(s) 349, 356, 440, 581, 693
MspR9I CCNGG 3 cut(s) 251, 452, 694
MvaI CCWGG 2 cut(s) 251, 452
MvnI CGCG 2 cut(s) 365, 420
MwoI GCNNNNNNNGC 5 cut(s) 253, 371, 482, 491, 653
NciI CCSGG 1 cut(s) 694
NcoI CCATGG 1 cut(s) 802
NlaIII CATG 4 cut(s) 572, 806, 841, 984
NlaIV GGNNCC 2 cut(s) 70, 248
NmeAIII GCCGAG 1 cut(s) 260
NmuCI GTSAC 3 cut(s) 433, 563, 599
OliI CACNNNNGTG 1 cut(s) 440
PaqCI CACCTGC 1 cut(s) 716
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 3 cut(s) 379, 940, 1049
PkrI GCNGC 5 cut(s) 246, 549, 552, 787, 1072
PleI GAGTC 2 cut(s) 178, 596
PpsI GAGTC 2 cut(s) 178, 596
Ppu21I YACGTR 1 cut(s) 850
PsiI TTATAA 1 cut(s) 1032
Psp124BI GAGCTC 2 cut(s) 508, 531
Psp6I CCWGG 2 cut(s) 249, 450
PspFI CCCAGC 2 cut(s) 471, 993
PspGI CCWGG 2 cut(s) 249, 450
PspN4I GGNNCC 2 cut(s) 70, 248
PspPI GGNCC 2 cut(s) 448, 806
PstI CTGCAG 1 cut(s) 1025
PsuI RGATCY 2 cut(s) 966, 1017
RsaI GTAC 3 cut(s) 644, 962, 1081
RsaNI GTAC 3 cut(s) 643, 961, 1080
RseI CAYNNNNRTG 2 cut(s) 440, 573
SacI GAGCTC 2 cut(s) 508, 531
SaqAI TTAA 2 cut(s) 798, 948
SatI GCNGC 5 cut(s) 245, 548, 551, 786, 1071
Sau96I GGNCC 2 cut(s) 448, 806
SchI GAGTC 2 cut(s) 178, 596
ScrFI CCNGG 3 cut(s) 251, 452, 694
SduI GDGCHC 4 cut(s) 198, 508, 531, 869
SfcI CTRYAG 2 cut(s) 202, 1021
SgrAI CRCCGGYG 1 cut(s) 439
SinI GGWCC 1 cut(s) 448
SmiMI CAYNNNNRTG 2 cut(s) 440, 573
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SnaBI TACGTA 1 cut(s) 850
Sse9I AATT 5 cut(s) 713, 790, 888, 976, 1033
SsiI CCGC 9 cut(s) 48, 60, 232, 244, 618, 660, 830, 855, 879
SspMI CTAG 1 cut(s) 665
SstI GAGCTC 2 cut(s) 508, 531
StyD4I CCNGG 3 cut(s) 249, 450, 692
StyI CCWWGG 3 cut(s) 306, 802, 1053
TaaI ACNGT 4 cut(s) 278, 563, 960, 1066
TaiI ACGT 2 cut(s) 167, 852
TaqI TCGA 3 cut(s) 258, 717, 943
TasI AATT 5 cut(s) 713, 790, 888, 976, 1033
TauI GCSGC 1 cut(s) 247
TfiI GAWTC 3 cut(s) 379, 940, 1049
Tru1I TTAA 2 cut(s) 798, 948
Tru9I TTAA 2 cut(s) 798, 948
TseFI GTSAC 3 cut(s) 433, 563, 599
TseI GCWGC 4 cut(s) 547, 550, 785, 1070
Tsp45I GTSAC 3 cut(s) 433, 563, 599
TspDTI ATGAA 3 cut(s) 641, 826, 969
TspGWI ACGGA 3 cut(s) 419, 730, 912
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 3 cut(s) 713, 790, 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.