MD15G1019300.v1.1

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017078
Physical Location & Seq
Reverse (-)
1122211 .. 1124841
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1019300.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGATCATCTCGCCTCCCTCCTCCTCCTTCTCTTTCTCCTATCCTCCGCACTCGCCTCCGCCGCAGTTCCCAACGACGTCGACCCTCTCATCCAACAAGTCGTTTCGGAATCCGACGACGATCAGCTCCTCCACGCCGAGCGCCATTTCTCCAGCTTCAAAGCCACCTTCGGAAAGACCTACGCGACTCAGGAGGAGCACGACTACAGATTCGGCGTCTTCAAGGCCAACCTCCGCCGCGCCAAGCGGCACCAGGCGCTCGACCCTACCGCGGTCCACGGCGTCACCAAGTTCTCCGATCTCACTCCCAAGGAGTTTCGCCGGAACTTTCTCGGACTCAAACGTCGCCTCCGGCTTCCGGCCGACGCTAACAAGGCTCCGATACTTCCCACTGGTGATCTTCCCGCCGACTTTGATTGGCGCGACAAAGGTGCTGTCACGCCGGTGAAGGACCAGGGTTCTTGCGGGTCGTGCTGGGCGTTTAGTGCGACGGGAGCTTTGGAAGGTGCTCATTATTTGGAAACAGGAGAGCTCGTCAGTCTGAGTGAGCAGCAGCTTGTTGACTGTGACCATGAGTGTGATCCGGAAGAATACGGCTCGTGTGACTCGGGTTGCAGTGGCGGACTGATGAACAATGCCTTTGAGTACGCACTCAAGGCTGGTGGGCTAGAACGAGAGGAAGATTATCCTTACACCGGGACCGATGACACCTGCAAATTCGACAAGAGCAAAATTGTTTCTTCCGTGTCTAACTTCAGCGTTATTTCTACCGACGAAGATCAAATCGCTGCAAATTTGGTTAACCATGGCCCTCTTGCAGTTGGGATCAATGCGGTGTTCATGCAAACATACGTAGGCGGAGTTTCGTGCCCTTACATCTGCGGAAAGCGAATTGATCACGGAGTGCTTCTGGTGGGTTATGGTTCTTCGGGTTTCGCTCCCATCCGACTCAAGGAAAAACCTTACTGGATCTTGAAGAATTCATGGGGACAGAGCTGGGGAGAGCAGGGATATTACAAGATCTGCAGGGGTTATAATTCTTGTGGGGTGGATTCCTTGGTCTCCACTGTTGCTGCTCTGCATACATCCAACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04210 map04142 map04210
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

366

Amino Acids

39.91

Weight (kDa)

5.47

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 50 - 106 1.7e-15 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 134 - 356 1.1e-74 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16190
fragaria_vesca FvH4_2g38940
malus_domestica MD08G1021600.v1.1 MD15G1019300.v1.1
prunus_persica Prupe.1G371600_v2.0.a1
pyrus_communis pycom08g01710
rosa_chinensis RchiOBHm_Chr6g0302031
rosa_laevigata RLG00000011201
rosa_roxburghii Rroxscaffold_7G00166170
rosa_rugosa Rorug06G0311100
rosa_samantha Rh6BG478400 Rh6DG424000
rosa_wichuraiana Rw6G036720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 342
AatII GACGTC 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 81
AccII CGCG 4 cut(s) 185, 240, 272, 423
AccIII TCCGGA 1 cut(s) 583
AclWI GGATC 4 cut(s) 12, 575, 833, 977
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 3 cut(s) 716, 793, 979
AcuI CTGAAG 1 cut(s) 739
AcyI GRCGYC 3 cut(s) 78, 216, 282
AdeI CACNNNGTG 1 cut(s) 904
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 4 cut(s) 271, 358, 695, 952
AgsI TTSAA 3 cut(s) 160, 223, 976
AjnI CCWGG 2 cut(s) 252, 453
AleI CACNNNNGTG 1 cut(s) 443
AluBI AGCT 6 cut(s) 127, 156, 497, 532, 556, 996
AluI AGCT 6 cut(s) 127, 156, 497, 532, 556, 996
Alw21I GWGCWC 3 cut(s) 201, 511, 534
Alw26I GTCTC 1 cut(s) 1066
AlwI GGATC 4 cut(s) 12, 575, 833, 977
Ama87I CYCGRG 1 cut(s) 607
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 3 cut(s) 225, 360, 808
ApeKI GCWGC 4 cut(s) 550, 553, 788, 1073
ApoI RAATTY 3 cut(s) 716, 793, 979
ArsI GACNNNNNNTTYG 6 cut(s) 194, 226, 420, 452, 481, 513
AspLEI GCGC 4 cut(s) 144, 242, 259, 423
AspS9I GGNCC 4 cut(s) 274, 451, 699, 809
AsuC2I CCSGG 1 cut(s) 697
AsuHPI GGTGA 3 cut(s) 277, 407, 457
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 3 cut(s) 274, 451, 699
BaeGI GKGCMC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 249
BanII GRGCYC 1 cut(s) 534
BauI CACGAG 1 cut(s) 598
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 3 cut(s) 201, 511, 534
BbvI GCAGC 4 cut(s) 562, 565, 775, 1060
BccI CCATC 1 cut(s) 950
BceAI ACGGC 2 cut(s) 295, 610
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 2 cut(s) 254, 455
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 1066
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 2 cut(s) 205, 1024
BfoI RGCGCY 2 cut(s) 145, 260
BfuAI ACCTGC 1 cut(s) 719
BglII AGATCT 1 cut(s) 1020
BisI GCNGC 7 cut(s) 63, 238, 248, 551, 554, 789, 1074
BlsI GCNGC 7 cut(s) 64, 239, 249, 552, 555, 790, 1075
Bme1390I CCNGG 3 cut(s) 254, 455, 697
Bme18I GGWCC 3 cut(s) 274, 451, 699
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 4 cut(s) 274, 451, 699, 809
BmiI GGNNCC 3 cut(s) 251, 378, 700
BmrFI CCNGG 3 cut(s) 254, 455, 697
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 136
BpuEI CTTGAG 2 cut(s) 638, 935
BpuMI CCSGG 1 cut(s) 697
BsaAI YACGTR 1 cut(s) 853
BsaHI GRCGYC 3 cut(s) 78, 216, 282
BsaI GGTCTC 1 cut(s) 1066
BsaJI CCNNGG 6 cut(s) 270, 277, 309, 454, 805, 1056
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 4 cut(s) 333, 363, 519, 549
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 358, 695, 952
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 2 cut(s) 397, 971
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 2 cut(s) 254, 455
BseDI CCNNGG 6 cut(s) 270, 277, 309, 454, 805, 1056
BseGI GGATG 3 cut(s) 90, 942, 1085
BseLI CCNNNNNNNGG 4 cut(s) 271, 358, 695, 952
BseMII CTCAG 2 cut(s) 203, 533
BseNI ACTGG 2 cut(s) 397, 971
BseRI GAGGAG 4 cut(s) 11, 14, 119, 209
BseSI GKGCMC 1 cut(s) 872
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 4 cut(s) 562, 565, 775, 1060
BseYI CCCAGC 2 cut(s) 474, 996
Bsh1236I CGCG 4 cut(s) 185, 240, 272, 423
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 3 cut(s) 227, 362, 810
BshNI GGYRCC 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 3 cut(s) 201, 511, 534
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 6 cut(s) 322, 352, 359, 443, 584, 696
BslFI GGGAC 2 cut(s) 712, 1002
BslI CCNNNNNNNGG 4 cut(s) 271, 358, 695, 952
BsmAI GTCTC 1 cut(s) 1066
BsmFI GGGAC 2 cut(s) 712, 1002
BsnI GGCC 3 cut(s) 227, 362, 810
Bso31I GGTCTC 1 cut(s) 1066
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 4 cut(s) 201, 511, 534, 872
Bsp13I TCCGGA 1 cut(s) 583
Bsp19I CCATGG 1 cut(s) 805
BspANI GGCC 3 cut(s) 227, 362, 810
BspCNI CTCAG 2 cut(s) 202, 534
BspEI TCCGGA 1 cut(s) 583
BspFNI CGCG 4 cut(s) 185, 240, 272, 423
BspLI GGNNCC 3 cut(s) 251, 378, 700
BspMAI CTGCAG 1 cut(s) 1028
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 4 cut(s) 12, 575, 833, 977
BspT107I GGYRCC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 1066
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 2 cut(s) 397, 971
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 6 cut(s) 270, 277, 309, 454, 805, 1056
BssNI GRCGYC 3 cut(s) 78, 216, 282
BssSI CACGAG 1 cut(s) 598
BssT1I CCWWGG 3 cut(s) 309, 805, 1056
Bst2BI CACGAG 1 cut(s) 598
Bst2UI CCWGG 2 cut(s) 254, 455
Bst4CI ACNGT 2 cut(s) 566, 1069
BstACI GRCGYC 3 cut(s) 78, 216, 282
BstBAI YACGTR 1 cut(s) 853
BstDEI CTNAG 2 cut(s) 189, 542
BstDSI CCRYGG 3 cut(s) 270, 277, 805
BstF5I GGATG 3 cut(s) 90, 942, 1085
BstFNI CGCG 4 cut(s) 185, 240, 272, 423
BstH2I RGCGCY 2 cut(s) 145, 260
BstHHI GCGC 4 cut(s) 144, 242, 259, 423
BstMAI GTCTC 1 cut(s) 1066
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 7 cut(s) 62, 256, 374, 471, 485, 494, 656
BstNI CCWGG 2 cut(s) 254, 455
BstSCI CCNGG 3 cut(s) 252, 453, 695
BstSFI CTRYAG 2 cut(s) 205, 1024
BstSLI GKGCMC 1 cut(s) 872
BstSNI TACGTA 1 cut(s) 853
BstUI CGCG 4 cut(s) 185, 240, 272, 423
BstV1I GCAGC 4 cut(s) 562, 565, 775, 1060
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 2 cut(s) 969, 1020
BstYI RGATCY 2 cut(s) 969, 1020
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 3 cut(s) 227, 362, 810
BtgI CCRYGG 3 cut(s) 270, 277, 805
BtsCI GGATG 3 cut(s) 90, 942, 1085
BtsI GCAGTG 1 cut(s) 622
BtsIMutI CAGTG 3 cut(s) 390, 622, 1065
BveI ACCTGC 1 cut(s) 719
CfoI GCGC 4 cut(s) 144, 242, 259, 423
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 4 cut(s) 274, 451, 699, 809
Cfr42I CCGCGG 1 cut(s) 273
CseI GACGC 3 cut(s) 205, 271, 374
Csp6I GTAC 1 cut(s) 646
CspCI CAANNNNNGTGG 2 cut(s) 643, 678
CviAII CATG 4 cut(s) 572, 806, 841, 984
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 189, 542
DraIII CACNNNGTG 1 cut(s) 904
DrdI GACNNNNNNGTC 1 cut(s) 342
DseDI GACNNNNNNGTC 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
EciI GGCGGA 4 cut(s) 49, 224, 636, 873
Ecl136II GAGCTC 1 cut(s) 532
EclXI CGGCCG 1 cut(s) 360
Eco105I TACGTA 1 cut(s) 853
Eco130I CCWWGG 3 cut(s) 309, 805, 1056
Eco24I GRGCYC 1 cut(s) 534
Eco31I GGTCTC 1 cut(s) 1066
Eco47I GGWCC 3 cut(s) 274, 451, 699
Eco52I CGGCCG 1 cut(s) 360
Eco53kI GAGCTC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 739
Eco88I CYCGRG 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 532
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 2 cut(s) 252, 453
EcoT14I CCWWGG 3 cut(s) 309, 805, 1056
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 3 cut(s) 309, 805, 1056
FaeI CATG 4 cut(s) 575, 809, 844, 987
FaiI YATR 8 cut(s) 573, 807, 842, 850, 921, 985, 1035, 1083
FaqI GGGAC 2 cut(s) 712, 1002
FatI CATG 4 cut(s) 571, 805, 840, 983
FauI CCCGC 2 cut(s) 412, 458
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 7 cut(s) 63, 238, 248, 551, 554, 789, 1074
FokI GGATG 3 cut(s) 77, 929, 1072
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 7 cut(s) 63, 238, 248, 551, 554, 789, 1074
FspBI CTAG 1 cut(s) 668
GlaI GCGC 4 cut(s) 143, 241, 258, 422
GluI GCNGC 7 cut(s) 63, 238, 248, 551, 554, 789, 1074
GsaI CCCAGC 2 cut(s) 478, 1000
GsuI CTGGAG 1 cut(s) 136
HaeII RGCGCY 2 cut(s) 145, 260
HaeIII GGCC 3 cut(s) 227, 362, 810
HapII CCGG 6 cut(s) 322, 352, 359, 443, 584, 696
HgaI GACGC 3 cut(s) 205, 271, 374
HhaI GCGC 4 cut(s) 144, 242, 259, 423
Hin1I GRCGYC 3 cut(s) 78, 216, 282
Hin1II CATG 4 cut(s) 575, 809, 844, 987
Hin6I GCGC 4 cut(s) 142, 240, 257, 421
HinP1I GCGC 4 cut(s) 142, 240, 257, 421
HincII GTYRAC 3 cut(s) 82, 562, 802
HindII GTYRAC 3 cut(s) 82, 562, 802
HinfI GANTC 7 cut(s) 110, 187, 210, 336, 605, 948, 1052
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 6 cut(s) 322, 352, 359, 443, 584, 696
HphI GGTGA 3 cut(s) 277, 407, 457
Hpy166II GTNNAC 4 cut(s) 82, 277, 562, 802
Hpy188I TCNGA 8 cut(s) 109, 115, 173, 298, 335, 381, 543, 947
Hpy188III TCNNGA 3 cut(s) 191, 584, 973
Hpy8I GTNNAC 4 cut(s) 82, 277, 562, 802
Hpy99I CGWCG 8 cut(s) 80, 83, 119, 122, 348, 368, 493, 776
HpyAV CCTTC 4 cut(s) 38, 178, 442, 497
HpyCH4III ACNGT 2 cut(s) 566, 1069
HpyCH4IV ACGT 3 cut(s) 78, 343, 852
HpyCH4V TGCA 7 cut(s) 615, 714, 791, 818, 844, 1026, 1081
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 256, 374, 471, 485, 494, 656
HpyF3I CTNAG 2 cut(s) 189, 542
HpySE526I ACGT 3 cut(s) 78, 343, 852
Hsp92I GRCGYC 3 cut(s) 78, 216, 282
Hsp92II CATG 4 cut(s) 575, 809, 844, 987
HspAI GCGC 4 cut(s) 142, 240, 257, 421
Kpn2I TCCGGA 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 895
KspAI GTTAAC 1 cut(s) 802
KspI CCGCGG 1 cut(s) 273
LmnI GCTCC 5 cut(s) 132, 196, 382, 494, 943
Lsp1109I GCAGC 4 cut(s) 562, 565, 775, 1060
MaeI CTAG 1 cut(s) 668
MaeII ACGT 3 cut(s) 78, 343, 852
MaeIII GTNAC 4 cut(s) 283, 436, 566, 602
MboII GAAGA 8 cut(s) 211, 392, 599, 692, 732, 788, 918, 988
MflI RGATCY 2 cut(s) 969, 1020
MhlI GDGCHC 4 cut(s) 201, 511, 534, 872
MluCI AATT 6 cut(s) 716, 732, 793, 891, 979, 1036
MlyI GAGTC 4 cut(s) 181, 330, 599, 942
MmeI TCCRAC 3 cut(s) 118, 138, 970
MroI TCCGGA 1 cut(s) 583
MseI TTAA 1 cut(s) 801
MslI CAYNNNNRTG 2 cut(s) 443, 576
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 6 cut(s) 322, 352, 359, 443, 584, 696
MspR9I CCNGG 3 cut(s) 254, 455, 697
MvaI CCWGG 2 cut(s) 254, 455
MvnI CGCG 4 cut(s) 185, 240, 272, 423
MwoI GCNNNNNNNGC 7 cut(s) 62, 256, 374, 471, 485, 494, 656
NciI CCSGG 1 cut(s) 697
NcoI CCATGG 1 cut(s) 805
NlaIII CATG 4 cut(s) 575, 809, 844, 987
NlaIV GGNNCC 3 cut(s) 251, 378, 700
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 4 cut(s) 283, 436, 566, 602
OliI CACNNNNGTG 1 cut(s) 443
PaqCI CACCTGC 1 cut(s) 719
PcsI WCGNNNNNNNCGW 1 cut(s) 476
PfeI GAWTC 3 cut(s) 110, 210, 1052
PkrI GCNGC 7 cut(s) 64, 239, 249, 552, 555, 790, 1075
PleI GAGTC 4 cut(s) 181, 330, 599, 942
PpsI GAGTC 4 cut(s) 181, 330, 599, 942
Ppu21I YACGTR 1 cut(s) 853
PsiI TTATAA 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 252, 453
PspFI CCCAGC 2 cut(s) 474, 996
PspGI CCWGG 2 cut(s) 252, 453
PspN4I GGNNCC 3 cut(s) 251, 378, 700
PspPI GGNCC 4 cut(s) 274, 451, 699, 809
PstI CTGCAG 1 cut(s) 1028
PsuI RGATCY 2 cut(s) 969, 1020
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
RseI CAYNNNNRTG 2 cut(s) 443, 576
SacI GAGCTC 1 cut(s) 534
SacII CCGCGG 1 cut(s) 273
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 801
SatI GCNGC 7 cut(s) 63, 238, 248, 551, 554, 789, 1074
Sau96I GGNCC 4 cut(s) 274, 451, 699, 809
SchI GAGTC 4 cut(s) 181, 330, 599, 942
ScrFI CCNGG 3 cut(s) 254, 455, 697
SduI GDGCHC 4 cut(s) 201, 511, 534, 872
SfcI CTRYAG 2 cut(s) 205, 1024
Sfr303I CCGCGG 1 cut(s) 273
SgrAI CRCCGGYG 1 cut(s) 442
SgrBI CCGCGG 1 cut(s) 273
SinI GGWCC 3 cut(s) 274, 451, 699
SmiMI CAYNNNNRTG 2 cut(s) 443, 576
SmlI CTYRAG 2 cut(s) 653, 950
SmoI CTYRAG 2 cut(s) 653, 950
SnaBI TACGTA 1 cut(s) 853
Sse9I AATT 6 cut(s) 716, 732, 793, 891, 979, 1036
SspMI CTAG 1 cut(s) 668
SstI GAGCTC 1 cut(s) 534
StyD4I CCNGG 3 cut(s) 252, 453, 695
StyI CCWWGG 3 cut(s) 309, 805, 1056
TaaI ACNGT 2 cut(s) 566, 1069
TaiI ACGT 3 cut(s) 81, 346, 855
TaqI TCGA 3 cut(s) 81, 261, 720
TaqII GACCGA 1 cut(s) 716
TasI AATT 6 cut(s) 716, 732, 793, 891, 979, 1036
TauI GCSGC 3 cut(s) 65, 240, 250
TfiI GAWTC 3 cut(s) 110, 210, 1052
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 3 cut(s) 397, 622, 1072
TseFI GTSAC 4 cut(s) 283, 436, 566, 602
TseI GCWGC 4 cut(s) 550, 553, 788, 1073
Tsp45I GTSAC 4 cut(s) 283, 436, 566, 602
TspDTI ATGAA 3 cut(s) 644, 829, 972
TspGWI ACGGA 2 cut(s) 733, 915
TspRI CASTG 3 cut(s) 397, 622, 1072
VpaK11BI GGWCC 3 cut(s) 274, 451, 699
XapI RAATTY 3 cut(s) 716, 793, 979
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 668
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.