MD08G1023000.v1.1

Glucose-induced degradation protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012908
Physical Location & Seq
Reverse (-)
1677633 .. 1680129
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1023000.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGACGTCGATCCTCGTCACTTCAATCAAGTTGCTATCAATGACAATGATATCAACAGTATTGTTCTGTCATACCTTGTGCATAACTGCTACAAAGAAACTGTGGAGTCGTTTGTTGCTTCGACGGGGACGAAGCAGCCTGCCGACTGCGTTGAGGATATGGAGAAAAGAAAAAGGATTTTTCAATGTGCAGTGGAGGGGAATGCTCTGAAGGCGATTGAACTGACGGAAGAGCTGGCACCTGAGTTACTGGAGAAAAACAAAGACTTGCATTTTGGCCTTCTTAGCCTGCACTTTGTTGAACTTGTTTGCGCTAAAAAATGCACAGAAGCTTTGGAATTCGCCCAGAACAAGTTGGCCCCCTTTGGGAAGGTGGACAAATACGTTACTAAGCTTGAAGATTTAATGGCTCTGCTTGCTTATGACGAACCGGAGAAGTCTCCTGTGTTTCATTTGCTTAGCTTGGATCATCGGCAAGAAGTTGCAGATAGTTTGAATCAAGCGATTCTTGGGCATTCAAACCTGCCCGATTACTCTGCATTGGAAAGGCTAATACAACAGACAACAGTAGTAAGACAATGTATGATTGAAGATAATGTCAAGAATGGGAATCCTCCATTTTCTTTGGAGGATTTTATTAGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH

Protein Analysis

216

Amino Acids

24.34

Weight (kDa)

4.94

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 54 - 192 2.3e-34 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012908)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06060 AT1G06060
fragaria_vesca FvH4_2g38860
malus_domestica MD08G1023000.v1.1 MD15G1020700.v1.1
prunus_persica Prupe.1G372300_v2.0.a1 Prupe.1G372300_v2.0.a1
pyrus_communis pycom08g01800 pycom15g01700
rosa_chinensis RchiOBHm_Chr6g0301961
rosa_laevigata RLG00000011207
rosa_multiflora Rmu_co8505221.1_g000001
rosa_roxburghii Rroxscaffold_7G00166240
rosa_rugosa Rorug06G0310600 Rorug06G0310600
rosa_samantha Rh6AG423000 Rh6BG429800
rosa_wichuraiana Rw6G036650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 238
AclWI GGATC 2 cut(s) 5, 474
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 8 cut(s) 25, 185, 221, 302, 398, 496, 519, 590
AluBI AGCT 5 cut(s) 235, 332, 394, 462, 645
AluI AGCT 5 cut(s) 235, 332, 394, 462, 645
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 2 cut(s) 5, 474
AoxI GGCC 2 cut(s) 277, 357
ApeKI GCWGC 1 cut(s) 136
ApoI RAATTY 1 cut(s) 338
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BcoDI GTCTC 1 cut(s) 444
BfuAI ACCTGC 1 cut(s) 531
BisI GCNGC 1 cut(s) 137
BlpI GCTNAGC 1 cut(s) 458
BlsI GCNGC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 240, 360
BpmI CTGGAG 1 cut(s) 272
Bpu1102I GCTNAGC 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 148
BsgI GTGCAG 2 cut(s) 210, 275
BshFI GGCC 2 cut(s) 279, 359
BshNI GGYRCC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 2 cut(s) 208, 514
BsnI GGCC 2 cut(s) 279, 359
Bsp143I GATC 2 cut(s) 10, 466
Bsp1720I GCTNAGC 1 cut(s) 458
BspANI GGCC 2 cut(s) 279, 359
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 240, 360
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 5, 474
BspQI GCTCTTC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 238
BsrI ACTGG 1 cut(s) 255
BssMI GATC 2 cut(s) 10, 466
BssNI GRCGYC 1 cut(s) 6
Bst4CI ACNGT 3 cut(s) 59, 103, 568
Bst6I CTCTTC 1 cut(s) 225
BstACI GRCGYC 1 cut(s) 6
BstC8I GCNNGC 4 cut(s) 141, 237, 290, 417
BstDEI CTNAG 4 cut(s) 243, 284, 390, 458
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 2 cut(s) 13, 469
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 2 cut(s) 10, 466
BstMWI GCNNNNNNNGC 3 cut(s) 212, 285, 416
BstV1I GCAGC 1 cut(s) 148
BsuRI GGCC 2 cut(s) 279, 359
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 4 cut(s) 141, 237, 290, 417
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 358
DdeI CTNAG 4 cut(s) 243, 284, 390, 458
DpnI GATC 2 cut(s) 12, 468
DpnII GATC 2 cut(s) 10, 466
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 338
EcoRV GATATC 1 cut(s) 52
FaiI YATR 5 cut(s) 73, 84, 161, 423, 584
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 137
Fsp4HI GCNGC 1 cut(s) 137
GlaI GCGC 1 cut(s) 313
GluI GCNGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 2 cut(s) 279, 359
HapII CCGG 1 cut(s) 431
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HindIII AAGCTT 2 cut(s) 330, 392
HinfI GANTC 4 cut(s) 107, 496, 505, 610
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 1 cut(s) 210
Hpy188III TCNNGA 1 cut(s) 601
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 2 cut(s) 11, 127
HpyAV CCTTC 3 cut(s) 205, 290, 364
HpyCH4III ACNGT 3 cut(s) 59, 103, 568
HpyCH4IV ACGT 2 cut(s) 6, 384
HpyCH4V TGCA 7 cut(s) 82, 191, 271, 292, 324, 485, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 285, 416
HpyF3I CTNAG 4 cut(s) 243, 284, 390, 458
HpySE526I ACGT 2 cut(s) 6, 384
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 10, 466
LguI GCTCTTC 1 cut(s) 225
LpnPI CCDG 9 cut(s) 153, 221, 236, 255, 302, 359, 444, 456, 536
Lsp1109I GCAGC 1 cut(s) 148
MaeII ACGT 2 cut(s) 6, 384
MaeIII GTNAC 3 cut(s) 17, 246, 385
MalI GATC 2 cut(s) 12, 468
MboI GATC 2 cut(s) 10, 466
MboII GAAGA 3 cut(s) 242, 410, 602
MluCI AATT 1 cut(s) 338
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 5 cut(s) 24, 148, 190, 622, 624
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 431
Mva1269I GAATGC 2 cut(s) 208, 514
MwoI GCNNNNNNNGC 3 cut(s) 212, 285, 416
NdeII GATC 2 cut(s) 10, 466
NlaIV GGNNCC 2 cut(s) 240, 360
NmuCI GTSAC 1 cut(s) 17
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 2 cut(s) 208, 514
PfeI GAWTC 3 cut(s) 496, 505, 610
PkrI GCNGC 1 cut(s) 138
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 240, 360
PspPI GGNCC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 2 cut(s) 10, 466
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 1 cut(s) 116
Sse9I AATT 1 cut(s) 338
TaaI ACNGT 3 cut(s) 59, 103, 568
TaiI ACGT 2 cut(s) 9, 387
TaqI TCGA 2 cut(s) 9, 122
TasI AATT 1 cut(s) 338
TfiI GAWTC 3 cut(s) 496, 505, 610
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 1 cut(s) 198
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 1 cut(s) 198
XapI RAATTY 1 cut(s) 338
ZraI GACGTC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.