MD15G1020700.v1.1

Glucose-induced degradation protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012908
Physical Location & Seq
Reverse (-)
1203721 .. 1206432
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1020700.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGACGTCGATCCTCGTCACTTCAATCAAGTTGCTATCAATGACAATGATATCCACAATATTGTTCTGTCATACCTTGTGCATAACTGCTATAAAGAAACTGTGGAGTCGTTTGTTGCTTCGACGGGGACGAAGCAGCCTGCCGACTGCGTTGAGGATATGGAGAAAAGAAATAGGATTTTTCAATGTAGAGTGGAAGGGGATGCTCTTAAGGCGATTGAACTGACGAAAGAGTTGGCACCTGACTTACTGGAGAAAAACAAAGACTTGCATTTTGACCTTCTGAGCCTGCACTTTGTTGAACTTGTTTGCGCTAAAAAATGCACAGAAGCTTTGGAATTTGCCCAGAACAAGTTGACCCCCTTTGGGAAGGTGGAAAAAGACGTTACAAAGCTTGAAGACTTCATGGCTCTGCTTGCTTATGAAGAACCGGAGAAGTCTCCTATGTTTCATTTGCTTAGCTTTGACTATCGGCAGCAAGTTGCAGACAGTTTGAATCGAGCAGTTCTTGAGCATTCAAACCTGCCCAATTATTCTGCACTGGAAAGGCTAATACAACAGACCACTGTAGTTAGACAATGCATGAGTGAAGATAATGCCAAGAATGGGAATCCACCATTTTCTCTGTATGATTTTCTTAGAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH

Protein Analysis

216

Amino Acids

24.7

Weight (kDa)

5.1

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 54 - 192 1.4e-32 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012908)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06060 AT1G06060
fragaria_vesca FvH4_2g38860
malus_domestica MD08G1023000.v1.1 MD15G1020700.v1.1
prunus_persica Prupe.1G372300_v2.0.a1 Prupe.1G372300_v2.0.a1
pyrus_communis pycom08g01800 pycom15g01700
rosa_chinensis RchiOBHm_Chr6g0301961
rosa_laevigata RLG00000011207
rosa_multiflora Rmu_co8505221.1_g000001
rosa_roxburghii Rroxscaffold_7G00166240
rosa_rugosa Rorug06G0310600 Rorug06G0310600
rosa_samantha Rh6AG423000 Rh6BG429800
rosa_wichuraiana Rw6G036650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 531
AccB1I GGYRCC 1 cut(s) 238
AclWI GGATC 1 cut(s) 5
AcsI RAATTY 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 2 cut(s) 366, 606
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 7 cut(s) 25, 185, 221, 302, 398, 496, 519
AluBI AGCT 4 cut(s) 332, 394, 462, 645
AluI AGCT 4 cut(s) 332, 394, 462, 645
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 136, 475
ApoI RAATTY 1 cut(s) 338
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 148, 487
BcoDI GTCTC 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 531
BisI GCNGC 2 cut(s) 137, 476
BlpI GCTNAGC 1 cut(s) 458
BlsI GCNGC 2 cut(s) 138, 477
BmiI GGNNCC 1 cut(s) 240
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 272
Bpu1102I GCTNAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 606
Bse1I ACTGG 2 cut(s) 255, 546
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 366, 606
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 255, 546
BseXI GCAGC 2 cut(s) 148, 487
BsgI GTGCAG 2 cut(s) 275, 522
BshNI GGYRCC 1 cut(s) 238
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 366, 606
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 142
BsmI GAATGC 1 cut(s) 514
Bsp143I GATC 1 cut(s) 10
Bsp1720I GCTNAGC 1 cut(s) 458
BspCNI CTCAG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 240
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 1 cut(s) 5
BspT107I GGYRCC 1 cut(s) 238
BspTI CTTAAG 1 cut(s) 209
BsrI ACTGG 2 cut(s) 255, 546
BssMI GATC 1 cut(s) 10
BssNI GRCGYC 1 cut(s) 6
Bst4CI ACNGT 3 cut(s) 103, 491, 568
BstACI GRCGYC 1 cut(s) 6
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 141, 290, 417
BstDEI CTNAG 3 cut(s) 284, 458, 638
BstF5I GGATG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 1 cut(s) 13
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 212, 416
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 148, 487
BstV2I GAAGAC 1 cut(s) 405
BtsCI GGATG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 539, 564
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 3 cut(s) 141, 290, 417
CfoI GCGC 1 cut(s) 314
CviAII CATG 2 cut(s) 406, 583
CviJI RGCY 8 cut(s) 139, 288, 332, 394, 410, 462, 550, 645
CviKI_1 RGCY 8 cut(s) 139, 288, 332, 394, 410, 462, 550, 645
DdeI CTNAG 3 cut(s) 284, 458, 638
DpnI GATC 1 cut(s) 12
DpnII GATC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 52
EcoRV GATATC 1 cut(s) 52
EcoT22I ATGCAT 1 cut(s) 584
FaeI CATG 2 cut(s) 409, 586
FaiI YATR 9 cut(s) 73, 84, 93, 161, 407, 423, 446, 584, 630
FaqI GGGAC 1 cut(s) 142
FatI CATG 2 cut(s) 405, 582
Fnu4HI GCNGC 2 cut(s) 137, 476
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 137, 476
GlaI GCGC 1 cut(s) 313
GluI GCNGC 2 cut(s) 137, 476
GsuI CTGGAG 1 cut(s) 272
HapII CCGG 1 cut(s) 431
HhaI GCGC 1 cut(s) 314
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 2 cut(s) 409, 586
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 2 cut(s) 330, 392
HinfI GANTC 3 cut(s) 107, 496, 610
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 11, 127
HpyAV CCTTC 3 cut(s) 191, 290, 364
HpyCH4III ACNGT 3 cut(s) 103, 491, 568
HpyCH4IV ACGT 2 cut(s) 6, 384
HpyCH4V TGCA 7 cut(s) 82, 271, 292, 324, 485, 539, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 416
HpyF3I CTNAG 3 cut(s) 284, 458, 638
HpySE526I ACGT 2 cut(s) 6, 384
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 2 cut(s) 409, 586
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 1 cut(s) 10
LpnPI CCDG 8 cut(s) 153, 236, 255, 302, 359, 444, 527, 536
Lsp1109I GCAGC 2 cut(s) 148, 487
LweI GCATC 1 cut(s) 193
MaeII ACGT 2 cut(s) 6, 384
MaeIII GTNAC 2 cut(s) 17, 385
MalI GATC 1 cut(s) 12
MboI GATC 1 cut(s) 10
MboII GAAGA 3 cut(s) 410, 437, 602
MluCI AATT 2 cut(s) 338, 529
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 2 cut(s) 24, 148
Mph1103I ATGCAT 1 cut(s) 584
MseI TTAA 1 cut(s) 210
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 514
MwoI GCNNNNNNNGC 2 cut(s) 212, 416
NdeII GATC 1 cut(s) 10
NlaIII CATG 2 cut(s) 409, 586
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 17
NsiI ATGCAT 1 cut(s) 584
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 1 cut(s) 514
PfeI GAWTC 2 cut(s) 496, 610
PkrI GCNGC 2 cut(s) 138, 477
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 2 cut(s) 137, 476
Sau3AI GATC 1 cut(s) 10
SchI GAGTC 1 cut(s) 116
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 567
SmlI CTYRAG 2 cut(s) 209, 509
SmoI CTYRAG 2 cut(s) 209, 509
Sse9I AATT 2 cut(s) 338, 529
SspI AATATT 1 cut(s) 61
TaaI ACNGT 3 cut(s) 103, 491, 568
TaiI ACGT 2 cut(s) 9, 387
TaqI TCGA 3 cut(s) 9, 122, 499
TasI AATT 2 cut(s) 338, 529
TfiI GAWTC 2 cut(s) 496, 610
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 2 cut(s) 546, 571
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 2 cut(s) 136, 475
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 3 cut(s) 394, 438, 440
TspRI CASTG 2 cut(s) 546, 571
Vha464I CTTAAG 1 cut(s) 209
XapI RAATTY 1 cut(s) 338
ZraI GACGTC 1 cut(s) 7
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.