MD08G1030200.v1.1

CBS domain-containing protein CBSX3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015428
Physical Location & Seq
Forward (+)
2174907 .. 2176258
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1030200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCAAGGAATTTCGCGGGCCGTGCGGTCATGCCGGGAAATACTCAAGTCTGCAATTTTACAGCATTCGCGTGGGGGTGAAGTTGCCGGGTTAGAAAAGATAGTTTCGCGTTTAAAATGGGTTACCTCTCCACCTGCACAGGAAAAGGGACTAGAGAATGTGACTGTGGCAGATGTACTGGCGACAAAGGGTAATGTAGAAACCGGACCCTGGCTCTGGTGTCACACGAACGACGCAGTAATTGATGCAGTAAAAAACATGGCGAAACAAAATATCGGGTCGTTACTGGTGCTGAAGCCAGGAGAGCATCAGCATATTGCAGGAATCATCACAGAAAGAGACTACCTGAGGAAAGTAATTGCACAGGAAAGATCTCCCATCTACACAAGAGTTGGAGAGATTATGACTAATGAGAACCAATTAATAACAGTAACTTCCGACATGAACGTTCTTCAAGCTATGCAACTTATGACAGAGAATCACATAAGGCATGCTCCAGTTATAGATGGGAAGCTAGTTGGCATGATTTCGATCAAGGATGTAGTCCGGGCAGTGGTTGAGCAGCAAAGCGGGGAACTGAAGCGACTTACTGGGTACATCAAAGGAGAATACTATTAG

Protein Analysis

206

Amino Acids

22.76

Weight (kDa)

8.85

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 131 - 186 1.5e-14 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015428)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47271
fragaria_vesca FvH4_2g39650
malus_domestica MD08G1030200.v1.1
prunus_persica Prupe.1G377900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0301241
rosa_laevigata RLG00000011268
rosa_multiflora Rmu_sc0013354.1_g000011
rosa_roxburghii Rroxscaffold_171G00441320 Rroxscaffold_7G00166990
rosa_rugosa Rorug06G0302600
rosa_samantha Rh6AG415900 Rh6AG416000 Rh6BG422700 Rh6DG415800 Rh6DG415900
rosa_wichuraiana Rw6G036020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 142
Acc36I ACCTGC 1 cut(s) 142
AccII CGCG 3 cut(s) 16, 70, 109
AciI CCGC 3 cut(s) 16, 25, 570
AclI AACGTT 1 cut(s) 447
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 2 cut(s) 314, 599
AfaI GTAC 2 cut(s) 177, 596
AfiI CCNNNNNNNGG 3 cut(s) 210, 216, 553
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 2 cut(s) 209, 298
AluBI AGCT 2 cut(s) 458, 514
AluI AGCT 2 cut(s) 458, 514
Alw26I GTCTC 1 cut(s) 333
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 562
ApoI RAATTY 1 cut(s) 9
AseI ATTAAT 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 18, 206
AsuC2I CCSGG 3 cut(s) 35, 88, 548
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 206
AxyI CCTNAGG 1 cut(s) 347
BbvI GCAGC 1 cut(s) 574
BccI CCATC 2 cut(s) 386, 500
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 2 cut(s) 211, 300
BcnI CCSGG 3 cut(s) 35, 88, 548
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 2 cut(s) 152, 515
BfuAI ACCTGC 1 cut(s) 142
BglII AGATCT 1 cut(s) 371
BisI GCNGC 1 cut(s) 563
BlsI GCNGC 1 cut(s) 564
Bme1390I CCNGG 5 cut(s) 35, 88, 211, 300, 548
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 18, 206
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 5 cut(s) 35, 88, 211, 300, 548
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 2 cut(s) 235, 316
BmuI ACTGGG 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 3 cut(s) 35, 88, 548
BsaBI GATNNNNATC 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 216, 553
Bse1I ACTGG 4 cut(s) 183, 291, 497, 595
Bse21I CCTNAGG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 2 cut(s) 211, 300
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 1 cut(s) 530
BseLI CCNNNNNNNGG 3 cut(s) 210, 216, 553
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 4 cut(s) 183, 291, 497, 595
BseXI GCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 3 cut(s) 16, 70, 109
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 4 cut(s) 34, 87, 204, 547
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 3 cut(s) 210, 216, 553
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 1 cut(s) 20
Bsp143I GATC 2 cut(s) 371, 531
BspACI CCGC 3 cut(s) 16, 25, 570
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 339
BspFNI CGCG 3 cut(s) 16, 70, 109
BspLI GGNNCC 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 142
BsrI ACTGG 4 cut(s) 183, 291, 497, 595
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 2 cut(s) 371, 531
Bst2UI CCWGG 2 cut(s) 211, 300
Bst4CI ACNGT 2 cut(s) 166, 430
BstC8I GCNNGC 2 cut(s) 18, 492
BstDEI CTNAG 1 cut(s) 347
BstEII GGTNACC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 3 cut(s) 16, 70, 109
BstKTI GATC 2 cut(s) 374, 534
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 371, 531
BstMWI GCNNNNNNNGC 2 cut(s) 22, 304
BstNI CCWGG 2 cut(s) 211, 300
BstNSI RCATGY 1 cut(s) 494
BstPI GGTNACC 1 cut(s) 121
BstSCI CCNGG 5 cut(s) 33, 86, 209, 298, 546
BstUI CGCG 3 cut(s) 16, 70, 109
BstV1I GCAGC 1 cut(s) 574
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
Bsu36I CCTNAGG 1 cut(s) 347
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 558
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 18, 492
Cfr13I GGNCC 2 cut(s) 18, 206
CseI GACGC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 176, 595
CviAII CATG 5 cut(s) 30, 259, 442, 491, 523
CviJI RGCY 5 cut(s) 20, 214, 298, 458, 514
CviKI_1 RGCY 5 cut(s) 20, 214, 298, 458, 514
CviQI GTAC 2 cut(s) 176, 595
DdeI CTNAG 1 cut(s) 347
DpnI GATC 2 cut(s) 373, 533
DpnII GATC 2 cut(s) 371, 531
DraI TTTAAA 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 314, 599
Eco81I CCTNAGG 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 209, 298
FaeI CATG 5 cut(s) 33, 262, 445, 494, 526
FaqI GGGAC 1 cut(s) 162
FatI CATG 5 cut(s) 29, 258, 441, 490, 522
FauI CCCGC 2 cut(s) 9, 563
Fnu4HI GCNGC 1 cut(s) 563
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 1 cut(s) 563
FspBI CTAG 2 cut(s) 152, 515
GluI GCNGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 1 cut(s) 20
HapII CCGG 4 cut(s) 34, 87, 204, 547
HgaI GACGC 1 cut(s) 242
Hin1II CATG 5 cut(s) 33, 262, 445, 494, 526
HinfI GANTC 2 cut(s) 324, 478
HpaII CCGG 4 cut(s) 34, 87, 204, 547
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 439
Hpy99I CGWCG 1 cut(s) 236
HpyCH4III ACNGT 2 cut(s) 166, 430
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 7 cut(s) 4, 53, 137, 248, 320, 362, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 304
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 5 cut(s) 33, 262, 445, 494, 526
Kzo9I GATC 2 cut(s) 371, 531
LmnI GCTCC 1 cut(s) 499
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 2 cut(s) 235, 316
MaeI CTAG 2 cut(s) 152, 515
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 5 cut(s) 121, 160, 221, 282, 430
MalI GATC 2 cut(s) 373, 533
MboI GATC 2 cut(s) 371, 531
MboII GAAGA 1 cut(s) 443
MflI RGATCY 1 cut(s) 371
MluCI AATT 5 cut(s) 9, 54, 240, 357, 419
MmeI TCCRAC 2 cut(s) 373, 462
MnlI CCTC 2 cut(s) 136, 342
MseI TTAA 2 cut(s) 113, 422
MslI CAYNNNNRTG 1 cut(s) 69
MspI CCGG 4 cut(s) 34, 87, 204, 547
MspR9I CCNGG 5 cut(s) 35, 88, 211, 300, 548
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 2 cut(s) 211, 300
MvnI CGCG 3 cut(s) 16, 70, 109
MwoI GCNNNNNNNGC 2 cut(s) 22, 304
NciI CCSGG 3 cut(s) 35, 88, 548
NdeII GATC 2 cut(s) 371, 531
NlaIII CATG 5 cut(s) 33, 262, 445, 494, 526
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 160, 221
NspI RCATGY 1 cut(s) 494
PaeI GCATGC 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 142
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 2 cut(s) 324, 478
PkrI GCNGC 1 cut(s) 564
PshBI ATTAAT 1 cut(s) 422
Psp1406I AACGTT 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 209, 298
PspEI GGTNACC 1 cut(s) 121
PspGI CCWGG 2 cut(s) 209, 298
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 18, 206
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 177, 596
RsaNI GTAC 2 cut(s) 176, 595
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 2 cut(s) 113, 422
SatI GCNGC 1 cut(s) 563
Sau3AI GATC 2 cut(s) 371, 531
Sau96I GGNCC 2 cut(s) 18, 206
ScrFI CCNGG 5 cut(s) 35, 88, 211, 300, 548
SetI ASST 6 cut(s) 128, 136, 348, 450, 460, 516
SfaNI GCATC 2 cut(s) 235, 316
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SphI GCATGC 1 cut(s) 494
Sse9I AATT 5 cut(s) 9, 54, 240, 357, 419
SsiI CCGC 3 cut(s) 16, 25, 570
SspMI CTAG 2 cut(s) 152, 515
StyD4I CCNGG 5 cut(s) 33, 86, 209, 298, 546
TaaI ACNGT 2 cut(s) 166, 430
TaiI ACGT 1 cut(s) 450
TaqI TCGA 1 cut(s) 530
TasI AATT 5 cut(s) 9, 54, 240, 357, 419
TatI WGTACW 1 cut(s) 175
TfiI GAWTC 2 cut(s) 324, 478
Tru1I TTAA 2 cut(s) 113, 422
Tru9I TTAA 2 cut(s) 113, 422
TscAI CASTG 1 cut(s) 558
TseFI GTSAC 2 cut(s) 160, 221
TseI GCWGC 1 cut(s) 562
Tsp45I GTSAC 2 cut(s) 160, 221
TspDTI ATGAA 1 cut(s) 458
TspRI CASTG 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 206
VspI ATTAAT 1 cut(s) 422
XapI RAATTY 1 cut(s) 9
XceI RCATGY 1 cut(s) 494
XspI CTAG 2 cut(s) 152, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.