Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008564

Physical Location & Seq

Genomic Coordinates Forward (+)

2276878 .. 2281363

Gene Structure 4486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1031800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGCAGTGGCAGTCGCTCGGTTAGCTCGGTTGGGCCGCAAAGCAAAACTGCCAGGTGGGTTCTGTATAAAAATGGCGGCGGCGGCAATCTTGGGCCTCTGCTTTATATTCGCCTGGTCGATGTTTTCTTCGCCCTCCTCCTCTGTCACCACCCGAATCGAAAGCTTCGACAACATTGTGGCGCCGCCTGGATCACGGAGCACCCGGGCGAGCACTCCCGATGGGACCCAACAAGCTGCTAGTAATGGTGGGGAGGAGAAAAGGGCTACTGGGTCTGCTGTGAGGTCACCTGTCCATGAGCATAAGTCCGAGAAGAAGGAGAAGAAAGTGGTAAATGGGAAGAAGGGGAAGGGAAAGAAAAAGTTACCGGAGAGTGTGACGAAGGTTAAAAATGTGACTGAGGAATCCGAGAGCGAAGATTCAGAGAACGAAAAGGAAGATGGAGATGAAGAAAAGGAGGTAGTGGATGGAAAGGAAGAAGCTTTGAGTGATGAAGGTGAAGGTAATGGGGAAGCAGGAGGAGAGGGGGGTAATTTGATGGAGACGGCGGATCAGGAATCCGAGGAGGTGAAGCTGGACGATGACGGTGGTGAATCCGAAAAAAAGGGAAAGAAGAGGTATAAGATGAAGGGTCCATTGTTTGATCCAAAAGCACATTATAGTTGGAAACAATGTAGCACAAGGAGCAAGCACAATTACATTCCTTGTATTGATATGGAAGTTGTATCTGGAAGGCTGCAGTATAGGCATACGGAGAGGAGTTGCCCGAAAGCGGAACCTATGTGTCTTGTACCTCTTCCCCGCGATGGATATGGCCTTCCGGTCCCCTGGCCTGAGAGCAAAGAGAAGATATTGTACAAGAATGTGGAGCATCCGAAACTTGCTGCATTCGTCAAGGAACATAGTTGGGTGATGGAGTCTGGAGAATATCTGACGTTCCCCCAAAACCAGTCTGAGTTGAAGGGCGGAATTCTTCACTATCTTGAGTCCATTGAAGAGATGGTACCAGACATCGAGTGGGGAAAGAATATCCGTGTAGTGCTGGATATTGGATGTACAGATTCTGCCTTTGGGGCTTCTCTCGTTGATAAGGATGTATTAACATTGGCATTGGGTTTGAAAGATGACCTAGTTGACCTAGCTCAAGTTGCCCTTGAGCGTGGTTTGCCTGCTTTTGTTAGCCCTTTTCGAAATAGAAGACTGCCTTTCCCCAGTAGTGTTTTTGATGCTGTTCACTGTGGCGGTTGCACCATACCTTGGCATTCAAATGGGGGTAAGCATCTTCTAGAGATGAATAGGATTTTAAGGCCAGGCGGATACTTTGTCTTGTCAACTAAACATGACAGCTTTGAAGATGAAGAAGCCATGACTAAATTGACCGCGTCTATCTGTTGGAATATTCTGGCTCATAAAACTGATGAAGTCAGTGACGTGGGTGTCAAAATATACCAGAAGCCAGATTCGAATGATATATATGAGCTGAGGAGGAAGAAATATCCTCCTTTGTGTAAGGAAAATGAAAACCCAGATGCAGCCTGGTATGTTCCCGTGAAGACTTGCTTGCACCCTATTCCATCTGCCATTGAGCAGCATGGCACGGAGTGGCCAGATGAATGGCCCCAAAGGCTCGAGAGGTATCCTGACTGGTTGAGTGATAAAGTTAACTTGGATGCCGACACCAAGCACTGGAAAGCTATTGTTGATAGATCCTATCTCACAGGACTAGGTATTGACTGGTCAAAAATTCGAAATGTAATGGACATGAAAGCCATCTATGGAGGATTTGCTGCAGCTCTCACTCAACAAAAGGTTTGGGTGATGAATGTGGTCCCTGTCCATGCGCCCGATACACTTCCTTTCATTTTTGAACGCGGCCTTGTTGGAACCTACCATGACTGGTGTGAGTCCTTCGGTACTTATCCCAGATCATATGATCTTTTGCATGTTGATCATCTGTTTTCAAGGCTTAAGAACAGGTGTAAGCAGCCTGTATCAATCGTTGTTGAGATGGATCGCTTGGTACGGCCTGGAGGCTGGGCAATTATACGCGAGAAAGTTGAGGTACTGGAACCGCTGGAGGGAATATTGAAGAGTATGCATTGGGAGATCCGGATGACTTTTGCTCAGGGAAAGGAGGGTATCTTATGCGCCCAGAAAACCTTGTGGCGGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF21 Methyltransf_29

Protein Analysis

725

Amino Acids

81.24

Weight (kDa)

6.15

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	232 - 719	2.5e-184	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11	PF08241	348 - 443	8.8e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19430 AT1G19430 AT1G19430
fragaria_vesca	FvH4_2g39800 FvH4_2g39800
malus_domestica	MD08G1031800.v1.1 MD15G1027700.v1.1
prunus_persica	Prupe.1G379400_v2.0.a1
pyrus_communis	pycom08g02510 pycom15g02360
rosa_chinensis	RchiOBHm_Chr6g0301041
rosa_laevigata	RLG00000011282
rosa_multiflora	Rmu_sc0003168.1_g000010 Rmu_sc0035551.1_g000001
rosa_roxburghii	Rroxscaffold_7G00167230
rosa_rugosa	Rorug06G0300200 Rorug06G0300300 Rorug06G0300400 Rorug06G0300500
rosa_samantha	Rh6AG414100 Rh6BG421100 Rh6CG428700 Rh6DG414100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1436
Acc65I	GGTACC	1 cut(s)	1003
AccB1I	GGYRCC	2 cut(s)	181, 1003
AccB7I	CCANNNNNTGG	2 cut(s)	1257, 1686
AccII	CGCG	4 cut(s)	804, 1382, 1872, 2049
AccIII	TCCGGA	1 cut(s)	2109
AclWI	GGATC	6 cut(s)	199, 558, 638, 1701, 2019, 2101
AcoI	YGGCCR	1 cut(s)	1604
AcsI	RAATTY	2 cut(s)	969, 1743
AcyI	GRCGYC	1 cut(s)	182
AdeI	CACNNNGTG	1 cut(s)	1602
AfaI	GTAC	7 cut(s)	792, 857, 1005, 1057, 1915, 2022, 2064
AfiI	CCNNNNNNNGG	6 cut(s)	772, 806, 1257, 1686, 2078, 2166
AflII	CTTAAG	1 cut(s)	1967
AgsI	TTSAA	8 cut(s)	961, 995, 1120, 1266, 1352, 1868, 1962, 2089
AjiI	CACGTC	1 cut(s)	1432
AjnI	CCWGG	7 cut(s)	52, 113, 187, 827, 1309, 1535, 2026
Alw21I	GWGCWC	2 cut(s)	203, 215
Alw26I	GTCTC	1 cut(s)	536
AlwI	GGATC	6 cut(s)	199, 558, 638, 1701, 2019, 2101
AlwNI	CAGNNNCTG	1 cut(s)	1064
Ama87I	CYCGRG	2 cut(s)	204, 1628
Aor13HI	TCCGGA	1 cut(s)	2109
ApeKI	GCWGC	8 cut(s)	236, 736, 884, 1532, 1588, 1787, 1790, 1984
ApoI	RAATTY	2 cut(s)	969, 1743
Asp718I	GGTACC	1 cut(s)	1003
AspLEI	GCGC	3 cut(s)	184, 1843, 2150
AspS9I	GGNCC	7 cut(s)	34, 94, 225, 632, 823, 1617, 1828
AsuC2I	CCSGG	2 cut(s)	205, 206
AsuHPI	GGTGA	7 cut(s)	139, 279, 509, 580, 602, 922, 1828
AsuII	TTCGAA	3 cut(s)	1189, 1463, 1747
AvaI	CYCGRG	2 cut(s)	204, 1628
AvaII	GGWCC	4 cut(s)	225, 632, 823, 1828
BalI	TGGCCA	1 cut(s)	1606
BanI	GGYRCC	2 cut(s)	181, 1003
BarI	GAAGNNNNNNTAC	4 cut(s)	839, 871, 987, 1019
BbsI	GAAGAC	2 cut(s)	1204, 1559
Bbv12I	GWGCWC	2 cut(s)	203, 215
BbvCI	CCTCAGC	1 cut(s)	1481
BbvI	GCAGC	8 cut(s)	223, 723, 871, 1544, 1600, 1774, 1802, 1996
BceAI	ACGGC	2 cut(s)	561, 2039
BciT130I	CCWGG	7 cut(s)	54, 115, 189, 829, 1311, 1537, 2028
BciVI	GTATCC	2 cut(s)	1310, 1647
BclI	TGATCA	1 cut(s)	1948
BcnI	CCSGG	2 cut(s)	205, 206
BcoDI	GTCTC	1 cut(s)	536
BfaI	CTAG	5 cut(s)	240, 1130, 1139, 1286, 1724
BfmI	CTRYAG	2 cut(s)	737, 1788
BfoI	RGCGCY	1 cut(s)	185
BfrI	CTTAAG	1 cut(s)	1967
BfuI	GTATCC	2 cut(s)	1310, 1647
BglI	GCCNNNNNGGC	2 cut(s)	1073, 1624
Bme1390I	CCNGG	9 cut(s)	54, 115, 189, 205, 206, 829, 1311, 1537, 2028
Bme18I	GGWCC	4 cut(s)	225, 632, 823, 1828
BmeT110I	CYCGRG	2 cut(s)	204, 1628
BmgBI	CACGTC	1 cut(s)	1432
BmgT120I	GGNCC	7 cut(s)	34, 94, 225, 632, 823, 1617, 1828
BmrFI	CCNGG	9 cut(s)	54, 115, 189, 205, 206, 829, 1311, 1537, 2028
BmrI	ACTGGG	2 cut(s)	279, 1206
BmsI	GCATC	5 cut(s)	880, 1216, 1288, 1519, 1660
BmuI	ACTGGG	2 cut(s)	279, 1206
BpiI	GAAGAC	2 cut(s)	1204, 1559
BpmI	CTGGAG	3 cut(s)	942, 2049, 2096
Bpu10I	CCTNAGC	2 cut(s)	1481, 2124
Bpu14I	TTCGAA	3 cut(s)	1189, 1463, 1747
BpuEI	CTTGAG	3 cut(s)	1004, 1128, 1175
BpuMI	CCSGG	2 cut(s)	205, 206
BsaHI	GRCGYC	1 cut(s)	182
BsaJI	CCNNGG	4 cut(s)	204, 561, 827, 1256
BsaWI	WCCGGW	3 cut(s)	367, 820, 2109
Bsc4I	CCNNNNNNNGG	6 cut(s)	772, 806, 1257, 1686, 2078, 2166
Bse1I	ACTGG	8 cut(s)	274, 949, 1212, 1649, 1691, 1739, 1901, 2070
BseAI	TCCGGA	1 cut(s)	2109
BseBI	CCWGG	7 cut(s)	54, 115, 189, 829, 1311, 1537, 2028
BseDI	CCNNGG	4 cut(s)	204, 561, 827, 1256
BseGI	GGATG	6 cut(s)	472, 871, 1058, 1099, 1675, 2118
BseLI	CCNNNNNNNGG	6 cut(s)	772, 806, 1257, 1686, 2078, 2166
BseMII	CTCAG	5 cut(s)	390, 825, 945, 1472, 2138
BseNI	ACTGG	8 cut(s)	274, 949, 1212, 1649, 1691, 1739, 1901, 2070
BseRI	GAGGAG	7 cut(s)	127, 130, 269, 534, 578, 772, 1498
BseXI	GCAGC	8 cut(s)	223, 723, 871, 1544, 1600, 1774, 1802, 1996
BseYI	CCCAGC	1 cut(s)	2034
Bsh1236I	CGCG	4 cut(s)	804, 1382, 1872, 2049
BshNI	GGYRCC	2 cut(s)	181, 1003
BsiHKAI	GWGCWC	2 cut(s)	203, 215
BsiHKCI	CYCGRG	2 cut(s)	204, 1628
BsiSI	CCGG	4 cut(s)	205, 368, 821, 2110
BslFI	GGGAC	3 cut(s)	238, 809, 1814
BslI	CCNNNNNNNGG	6 cut(s)	772, 806, 1257, 1686, 2078, 2166
BsmAI	GTCTC	1 cut(s)	536
BsmBI	CGTCTC	1 cut(s)	536
BsmFI	GGGAC	3 cut(s)	238, 809, 1814
BsmI	GAATGC	2 cut(s)	887, 1261
BsoBI	CYCGRG	2 cut(s)	204, 1628
Bsp119I	TTCGAA	3 cut(s)	1189, 1463, 1747
Bsp1286I	GDGCHC	2 cut(s)	203, 215
Bsp13I	TCCGGA	1 cut(s)	2109
Bsp1407I	TGTACA	2 cut(s)	855, 1055
Bsp143I	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
BspCNI	CTCAG	5 cut(s)	391, 826, 946, 1473, 2137
BspEI	TCCGGA	1 cut(s)	2109
BspFNI	CGCG	4 cut(s)	804, 1382, 1872, 2049
BspMAI	CTGCAG	2 cut(s)	741, 1792
BspPI	GGATC	6 cut(s)	199, 558, 638, 1701, 2019, 2101
BspT104I	TTCGAA	3 cut(s)	1189, 1463, 1747
BspT107I	GGYRCC	2 cut(s)	181, 1003
BspTI	CTTAAG	1 cut(s)	1967
BsrGI	TGTACA	2 cut(s)	855, 1055
BsrI	ACTGG	8 cut(s)	274, 949, 1212, 1649, 1691, 1739, 1901, 2070
BssECI	CCNNGG	4 cut(s)	204, 561, 827, 1256
BssMI	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
BssNI	GRCGYC	1 cut(s)	182
BssT1I	CCWWGG	1 cut(s)	1256
Bst2UI	CCWGG	7 cut(s)	54, 115, 189, 829, 1311, 1537, 2028
Bst4CI	ACNGT	2 cut(s)	587, 1238
Bst6I	CTCTTC	4 cut(s)	608, 801, 990, 2084
BstACI	GRCGYC	1 cut(s)	182
BstAFI	CTTAAG	1 cut(s)	1967
BstAUI	TGTACA	2 cut(s)	855, 1055
BstBI	TTCGAA	3 cut(s)	1189, 1463, 1747
BstC8I	GCNNGC	4 cut(s)	211, 689, 1170, 1562
BstDEI	CTNAG	5 cut(s)	399, 834, 954, 1481, 2124
BstEII	GGTNACC	1 cut(s)	285
BstF5I	GGATG	6 cut(s)	472, 871, 1058, 1099, 1675, 2118
BstFNI	CGCG	4 cut(s)	804, 1382, 1872, 2049
BstH2I	RGCGCY	1 cut(s)	185
BstHHI	GCGC	3 cut(s)	184, 1843, 2150
BstKTI	GATC	9 cut(s)	194, 553, 646, 1709, 1928, 1936, 1951, 2014, 2109
BstMAI	GTCTC	1 cut(s)	536
BstMBI	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
BstMWI	GCNNNNNNNGC	8 cut(s)	23, 83, 684, 745, 1073, 1148, 1165, 1624
BstNI	CCWGG	7 cut(s)	54, 115, 189, 829, 1311, 1537, 2028
BstNSI	RCATGY	1 cut(s)	1946
BstPI	GGTNACC	1 cut(s)	285
BstSCI	CCNGG	9 cut(s)	52, 113, 187, 203, 204, 827, 1309, 1535, 2026
BstSFI	CTRYAG	2 cut(s)	737, 1788
BstUI	CGCG	4 cut(s)	804, 1382, 1872, 2049
BstV1I	GCAGC	8 cut(s)	223, 723, 871, 1544, 1600, 1774, 1802, 1996
BstV2I	GAAGAC	2 cut(s)	1204, 1559
BstX2I	RGATCY	2 cut(s)	1706, 2106
BstXI	CCANNNNNNTGG	1 cut(s)	1614
BstYI	RGATCY	2 cut(s)	1706, 2106
BsuI	GTATCC	2 cut(s)	1310, 1647
BtgZI	GCGATG	1 cut(s)	819
BtrI	CACGTC	1 cut(s)	1432
BtsCI	GGATG	6 cut(s)	472, 871, 1058, 1099, 1675, 2118
BtsI	GCAGTG	1 cut(s)	12
BtsIMutI	CAGTG	4 cut(s)	12, 1234, 1432, 1684
Cac8I	GCNNGC	4 cut(s)	211, 689, 1170, 1562
CaiI	CAGNNNCTG	1 cut(s)	1064
CfoI	GCGC	3 cut(s)	184, 1843, 2150
Cfr13I	GGNCC	7 cut(s)	34, 94, 225, 632, 823, 1617, 1828
Cfr9I	CCCGGG	1 cut(s)	204
CseI	GACGC	1 cut(s)	1371
Csp6I	GTAC	7 cut(s)	791, 856, 1004, 1056, 1914, 2021, 2063
CviAII	CATG	8 cut(s)	296, 1340, 1366, 1592, 1762, 1838, 1892, 1943
CviQI	GTAC	7 cut(s)	791, 856, 1004, 1056, 1914, 2021, 2063
DdeI	CTNAG	5 cut(s)	399, 834, 954, 1481, 2124
DinI	GGCGCC	1 cut(s)	183
DpnI	GATC	9 cut(s)	193, 552, 645, 1708, 1927, 1935, 1950, 2013, 2108
DpnII	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
DraIII	CACNNNGTG	1 cut(s)	1602
DrdI	GACNNNNNNGTC	1 cut(s)	1436
DseDI	GACNNNNNNGTC	1 cut(s)	1436
EaeI	YGGCCR	1 cut(s)	1604
Eam1104I	CTCTTC	4 cut(s)	608, 801, 990, 2084
EarI	CTCTTC	4 cut(s)	608, 801, 990, 2084
EciI	GGCGGA	3 cut(s)	563, 981, 1329
Eco130I	CCWWGG	1 cut(s)	1256
Eco47I	GGWCC	4 cut(s)	225, 632, 823, 1828
Eco88I	CYCGRG	2 cut(s)	204, 1628
Eco91I	GGTNACC	1 cut(s)	285
EcoO109I	RGGNCCY	1 cut(s)	225
EcoO65I	GGTNACC	1 cut(s)	285
EcoRI	GAATTC	1 cut(s)	969
EcoRII	CCWGG	7 cut(s)	52, 113, 187, 827, 1309, 1535, 2026
EcoT14I	CCWWGG	1 cut(s)	1256
EcoT22I	ATGCAT	1 cut(s)	2100
EgeI	GGCGCC	1 cut(s)	183
EheI	GGCGCC	1 cut(s)	183
ErhI	CCWWGG	1 cut(s)	1256
Esp3I	CGTCTC	1 cut(s)	536
FaeI	CATG	8 cut(s)	299, 1343, 1369, 1595, 1765, 1841, 1895, 1946
FalI	AAGNNNNNCTT	6 cut(s)	1137, 1169, 1189, 1221, 1544, 1576
FaqI	GGGAC	3 cut(s)	238, 809, 1814
FatI	CATG	8 cut(s)	295, 1339, 1365, 1591, 1761, 1837, 1891, 1942
FauI	CCCGC	1 cut(s)	809
FauNDI	CATATG	1 cut(s)	1930
FbaI	TGATCA	1 cut(s)	1948
FokI	GGATG	6 cut(s)	479, 858, 1065, 1106, 1682, 2125
FspBI	CTAG	5 cut(s)	240, 1130, 1139, 1286, 1724
GlaI	GCGC	3 cut(s)	183, 1842, 2149
GsaI	CCCAGC	1 cut(s)	2038
GsuI	CTGGAG	3 cut(s)	942, 2049, 2096
HaeII	RGCGCY	1 cut(s)	185
HapII	CCGG	4 cut(s)	205, 368, 821, 2110
HgaI	GACGC	1 cut(s)	1371
HhaI	GCGC	3 cut(s)	184, 1843, 2150
Hin1I	GRCGYC	1 cut(s)	182
Hin1II	CATG	8 cut(s)	299, 1343, 1369, 1595, 1765, 1841, 1895, 1946
Hin6I	GCGC	3 cut(s)	182, 1841, 2148
HinP1I	GCGC	3 cut(s)	182, 1841, 2148
HincII	GTYRAC	3 cut(s)	1135, 1332, 1663
HindII	GTYRAC	3 cut(s)	1135, 1332, 1663
HindIII	AAGCTT	2 cut(s)	163, 480
HpaI	GTTAAC	1 cut(s)	1663
HpaII	CCGG	4 cut(s)	205, 368, 821, 2110
HphI	GGTGA	7 cut(s)	139, 279, 509, 580, 602, 922, 1828
Hpy166II	GTNNAC	4 cut(s)	1135, 1234, 1332, 1663
Hpy188I	TCNGA	8 cut(s)	310, 409, 424, 562, 598, 876, 933, 955
Hpy188III	TCNNGA	9 cut(s)	218, 554, 729, 921, 983, 1286, 1630, 1640, 2110
Hpy8I	GTNNAC	4 cut(s)	1135, 1234, 1332, 1663
HpyCH4III	ACNGT	2 cut(s)	587, 1238
HpyCH4IV	ACGT	2 cut(s)	935, 1431
HpyCH4V	TGCA	8 cut(s)	739, 887, 1248, 1532, 1564, 1790, 1942, 2098
HpyF10VI	GCNNNNNNNGC	8 cut(s)	23, 83, 684, 745, 1073, 1148, 1165, 1624
HpyF3I	CTNAG	5 cut(s)	399, 834, 954, 1481, 2124
HpySE526I	ACGT	2 cut(s)	935, 1431
Hsp92I	GRCGYC	1 cut(s)	182
Hsp92II	CATG	8 cut(s)	299, 1343, 1369, 1595, 1765, 1841, 1895, 1946
HspAI	GCGC	3 cut(s)	182, 1841, 2148
KasI	GGCGCC	1 cut(s)	181
KflI	GGGWCCC	1 cut(s)	225
Kpn2I	TCCGGA	1 cut(s)	2109
KpnI	GGTACC	1 cut(s)	1007
Ksp22I	TGATCA	1 cut(s)	1948
KspAI	GTTAAC	1 cut(s)	1663
Kzo9I	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
LmnI	GCTCC	3 cut(s)	198, 684, 868
Lsp1109I	GCAGC	8 cut(s)	223, 723, 871, 1544, 1600, 1774, 1802, 1996
LweI	GCATC	5 cut(s)	880, 1216, 1288, 1519, 1660
MaeI	CTAG	5 cut(s)	240, 1130, 1139, 1286, 1724
MaeII	ACGT	2 cut(s)	935, 1431
MaeIII	GTNAC	6 cut(s)	145, 285, 363, 376, 394, 1427
MalI	GATC	9 cut(s)	193, 552, 645, 1708, 1927, 1935, 1950, 2013, 2108
MboI	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
MflI	RGATCY	2 cut(s)	1706, 2106
MhlI	GDGCHC	2 cut(s)	203, 215
MlsI	TGGCCA	1 cut(s)	1606
MluCI	AATT	6 cut(s)	532, 694, 969, 1373, 1743, 2040
MluNI	TGGCCA	1 cut(s)	1606
Mly113I	GGCGCC	1 cut(s)	182
MlyI	GAGTC	3 cut(s)	926, 995, 1913
MmeI	TCCRAC	3 cut(s)	644, 1373, 1861
Mox20I	TGGCCA	1 cut(s)	1606
Mph1103I	ATGCAT	1 cut(s)	2100
MroI	TCCGGA	1 cut(s)	2109
MscI	TGGCCA	1 cut(s)	1606
MseI	TTAA	6 cut(s)	387, 1100, 1304, 1662, 1968, 2173
MslI	CAYNNNNRTG	1 cut(s)	1266
Msp20I	TGGCCA	1 cut(s)	1606
MspA1I	CMGCKG	1 cut(s)	2074
MspCI	CTTAAG	1 cut(s)	1967
MspI	CCGG	4 cut(s)	205, 368, 821, 2110
MspR9I	CCNGG	9 cut(s)	54, 115, 189, 205, 206, 829, 1311, 1537, 2028
Mva1269I	GAATGC	2 cut(s)	887, 1261
MvaI	CCWGG	7 cut(s)	54, 115, 189, 829, 1311, 1537, 2028
MvnI	CGCG	4 cut(s)	804, 1382, 1872, 2049
MwoI	GCNNNNNNNGC	8 cut(s)	23, 83, 684, 745, 1073, 1148, 1165, 1624
NarI	GGCGCC	1 cut(s)	182
NciI	CCSGG	2 cut(s)	205, 206
NdeI	CATATG	1 cut(s)	1930
NdeII	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
NlaIII	CATG	8 cut(s)	299, 1343, 1369, 1595, 1765, 1841, 1895, 1946
NmuCI	GTSAC	5 cut(s)	145, 285, 376, 394, 1427
NsiI	ATGCAT	1 cut(s)	2100
NspI	RCATGY	1 cut(s)	1946
NspV	TTCGAA	3 cut(s)	1189, 1463, 1747
PaeR7I	CTCGAG	1 cut(s)	1628
PcsI	WCGNNNNNNNCGW	1 cut(s)	165
PctI	GAATGC	2 cut(s)	887, 1261
PfeI	GAWTC	7 cut(s)	156, 404, 419, 557, 593, 1061, 1460
PflFI	GACNNNGTC	1 cut(s)	1381
PflMI	CCANNNNNTGG	2 cut(s)	1257, 1686
PleI	GAGTC	3 cut(s)	925, 994, 1912
PluTI	GGCGCC	1 cut(s)	185
PpsI	GAGTC	3 cut(s)	925, 994, 1912
PpuMI	RGGWCCY	1 cut(s)	225
Psp5II	RGGWCCY	1 cut(s)	225
Psp6I	CCWGG	7 cut(s)	52, 113, 187, 827, 1309, 1535, 2026
PspEI	GGTNACC	1 cut(s)	285
PspFI	CCCAGC	1 cut(s)	2034
PspGI	CCWGG	7 cut(s)	52, 113, 187, 827, 1309, 1535, 2026
PspPI	GGNCC	7 cut(s)	34, 94, 225, 632, 823, 1617, 1828
PspPPI	RGGWCCY	1 cut(s)	225
PstI	CTGCAG	2 cut(s)	741, 1792
PstNI	CAGNNNCTG	1 cut(s)	1064
PsuI	RGATCY	2 cut(s)	1706, 2106
PsyI	GACNNNGTC	1 cut(s)	1381
RsaI	GTAC	7 cut(s)	792, 857, 1005, 1057, 1915, 2022, 2064
RsaNI	GTAC	7 cut(s)	791, 856, 1004, 1056, 1914, 2021, 2063
RseI	CAYNNNNRTG	1 cut(s)	1266
SaqAI	TTAA	6 cut(s)	387, 1100, 1304, 1662, 1968, 2173
Sau3AI	GATC	9 cut(s)	191, 550, 643, 1706, 1925, 1933, 1948, 2011, 2106
Sau96I	GGNCC	7 cut(s)	34, 94, 225, 632, 823, 1617, 1828
SchI	GAGTC	3 cut(s)	926, 995, 1913
ScrFI	CCNGG	9 cut(s)	54, 115, 189, 205, 206, 829, 1311, 1537, 2028
SduI	GDGCHC	2 cut(s)	203, 215
SfaNI	GCATC	5 cut(s)	880, 1216, 1288, 1519, 1660
SfcI	CTRYAG	2 cut(s)	737, 1788
SfoI	GGCGCC	1 cut(s)	183
Sfr274I	CTCGAG	1 cut(s)	1628
SfuI	TTCGAA	3 cut(s)	1189, 1463, 1747
SinI	GGWCC	4 cut(s)	225, 632, 823, 1828
SlaI	CTCGAG	1 cut(s)	1628
SmaI	CCCGGG	1 cut(s)	206
SmiMI	CAYNNNNRTG	1 cut(s)	1266
SmlI	CTYRAG	5 cut(s)	983, 1143, 1154, 1628, 1967
SmoI	CTYRAG	5 cut(s)	983, 1143, 1154, 1628, 1967
Sse9I	AATT	6 cut(s)	532, 694, 969, 1373, 1743, 2040
SspDI	GGCGCC	1 cut(s)	181
SspI	AATATT	2 cut(s)	1399, 2085
SspMI	CTAG	5 cut(s)	240, 1130, 1139, 1286, 1724
StyD4I	CCNGG	9 cut(s)	52, 113, 187, 203, 204, 827, 1309, 1535, 2026
StyI	CCWWGG	1 cut(s)	1256
TaaI	ACNGT	2 cut(s)	587, 1238
TaiI	ACGT	2 cut(s)	938, 1434
TaqI	TCGA	8 cut(s)	119, 159, 168, 1014, 1189, 1463, 1629, 1747
TasI	AATT	6 cut(s)	532, 694, 969, 1373, 1743, 2040
TatI	WGTACW	2 cut(s)	855, 1055
TauI	GCSGC	7 cut(s)	39, 80, 83, 86, 187, 1875, 2170
TfiI	GAWTC	7 cut(s)	156, 404, 419, 557, 593, 1061, 1460
Tru1I	TTAA	6 cut(s)	387, 1100, 1304, 1662, 1968, 2173
Tru9I	TTAA	6 cut(s)	387, 1100, 1304, 1662, 1968, 2173
TscAI	CASTG	4 cut(s)	12, 1241, 1432, 1691
TseFI	GTSAC	5 cut(s)	145, 285, 376, 394, 1427
TseI	GCWGC	8 cut(s)	236, 736, 884, 1532, 1588, 1787, 1790, 1984
Tsp45I	GTSAC	5 cut(s)	145, 285, 376, 394, 1427
TspGWI	ACGGA	4 cut(s)	211, 767, 1022, 1613
TspMI	CCCGGG	1 cut(s)	204
TspRI	CASTG	4 cut(s)	12, 1241, 1432, 1691
Tth111I	GACNNNGTC	1 cut(s)	1381
Van91I	CCANNNNNTGG	2 cut(s)	1257, 1686
Vha464I	CTTAAG	1 cut(s)	1967
VpaK11BI	GGWCC	4 cut(s)	225, 632, 823, 1828
XapI	RAATTY	2 cut(s)	969, 1743
XbaI	TCTAGA	1 cut(s)	1285
XceI	RCATGY	1 cut(s)	1946
XcmI	CCANNNNNNNNNTGG	3 cut(s)	997, 1014, 1533
XhoI	CTCGAG	1 cut(s)	1628
XmaI	CCCGGG	1 cut(s)	204
XspI	CTAG	5 cut(s)	240, 1130, 1139, 1286, 1724
Zsp2I	ATGCAT	1 cut(s)	2100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.