Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0008564

Physical Location & Seq

Genomic Coordinates Reverse (-)

64448034 .. 64451624

Gene Structure 3591 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG421100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGGCGGTAGCTGCAGCTCGGTTTGGTCGCCAAGCGAAACGCCCGTATTGGGGTTTCTGTATAAAGATGACAGCGGTGGCCGTTATGGGTCTCTGCTTTATATTTGTGTGGACTATGTTTACGTCTCCTTCGAATTCAGTGAGTACAAGAATAGAGAGCTTTGACAATATAGAGCAGCCTCCTGGATCAAGGAGCAAAAGGGTGAGCAGTTCTGAGAGGAAGGTAAATGGGACGTCTGTTGCTTTGGGTGGTCATGAGGTTAAATCTGAGGAGAGGGAGAAGAAGGAAGTGGCGAATACGAAAAAGGAGAGAGTTAAGAAGAAGTTACCAGAGAAGGTGTCGAAAGAGAAACAATTGGGGAGTAAGGAAAAGGAGAAGGAAGATGGAGATGAAGATGAAGAGCCAGAAGTGGTGGATGGAAAGGAAGAAGGTTTGGATGGTGAAGATGAAGGTGAAGGTGAAGGAAATGGGAATGCGGAAGGAGAGGGTGATTTGATTGAAGGGGTGGATCAGGAATTTGAGGAGAAGTTGGAAGATGATGGCGGTGAATCGGAGAAGAGGGGAAAGAAGAGAAATAAGATTAAGGGTCCGGTGTTTGATCCGAAAGCACATTATCATTGGAAATCATGTAGCACAAGGAGCAAGCACAATTACATTCCTTGTATTGATATAGAAGTTGTCTCTGGAAGGCTGCAGCAATATAGGCACACAGAGAGGAGTTGTCCACGATCACCGCCAATGTGTCTTGTGCCTCTTCCTCCAGATGGGTATGATCCTCCAGTCCATTGGCCCGAGAGTAAAGAGAAGATATTGTATAAGAACGTGGAGCATCCAAAGCTTGCTGCATTCGTCAAGGAACATACTTGGGTGATGGAGTCTGGTGAATACCTTACTTTTCCTCAAAACCAGTCTGAGATTAAGGGTGGGGTTCTTCACTATCTTGAGTCCATTGAAGAGATGGTACCAGATCTAGAATGGGGAAAGAATATCCGTGTAGTGCTGGATATCGGATGTACAGATTCCGCTTTTGGGGCTTCTCTCCTTGATAAGGATGTATTAACATTATCATTGGGGTTGAAAGATGACCTAGTTGACCTAGCTCAAGTTTCCCTCGAGCGTGGTTTTCCTACTGTAGTTAGCCCATTCGGAAACAGAAGGCTAGCTTTCCCTAGTGGTGTTTTTGATGTTATTCACTGCGGCGGGTGCACTGTACCTTGGCATTCAAATGGGGGTAAGCATCTTCTAGAGATGAATAGGATTTTAAGGCCCGGGGGATACTTTATCTTGTCAACTAAACATGACAGCTTTGAAGCTGAAGAAGCCGTGAATACATTGATGGTATCTATCTGCTGGAATATTTTGGCTCATAAAACTGATGAAGTCAGTGATGTGGGTGTCAAAATATATAAGAAGCCGGACTCAAATGATTTATATACGATGAGGAGGAAGAAACATTGGATACCTTTATGCAAGGGAAATGATAACCCAGATGCAGCCTGGTATGTCCCGGTGACGACTTGTTTGCACACCATTCCATCTGGTATTGAAGAGCGTGGATCAGAGTGGCCGGAGGAATGGCCCAACAGGCTCGAAAGATATCCGGACTGGTTGAACAATAAAGATAAGTTGATTGCAGACACCAAGCACTGGAAAGATATTGTTGACAAATCCTATCTTATTGGACTTGGTATTGACTGGTCAAGCATTCGAAATGTAATGGACATGAAAGCCATCTATGGAGGATTTGCTGCAGCCCTCTTGCAACAAAAGGTTTGGGTGATGAATGTGGTCCCTGTCCATGCACCTGATACGCTTCCTTTCATTTTTGAGCGTGGGCTTGTTGGAACCTACCACGACTGGTGTGAGTCTTTCGGTACTTATCCAAGATCATATGATCTTTTGCATGCTGATCATCTGTTTTCACGGCTCAAGAACAGGTGTAAGCAGCCTGCATCAATTGTTGTTGAGATGGATCGTATTTTACGGCCAGGAGGTTGGGCCATCATACGTGACAAAGTTGAGATTCTAGATCCGTTGGAGGGAATACTCAAGAGTTTGCATTGGGATATAAGAATGACTTATGCTCAACAAAAAGAGGGTATTCTCTGTGCACAGAAAACTACATGGCGCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

711

Amino Acids

80.61

Weight (kDa)

5.94

Isoelectric Point (pI)

47.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	217 - 706	5.3e-184	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008564)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19430 AT1G19430 AT1G19430
fragaria_vesca	FvH4_2g39800 FvH4_2g39800
malus_domestica	MD08G1031800.v1.1 MD15G1027700.v1.1
prunus_persica	Prupe.1G379400_v2.0.a1
pyrus_communis	pycom08g02510 pycom15g02360
rosa_chinensis	RchiOBHm_Chr6g0301041
rosa_laevigata	RLG00000011282
rosa_multiflora	Rmu_sc0003168.1_g000010 Rmu_sc0035551.1_g000001
rosa_roxburghii	Rroxscaffold_7G00167230
rosa_rugosa	Rorug06G0300200 Rorug06G0300300 Rorug06G0300400 Rorug06G0300500
rosa_samantha	Rh6AG414100 Rh6BG421100 Rh6CG428700 Rh6DG414100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	236
Acc65I	GGTACC	1 cut(s)	961
AccB1I	GGYRCC	2 cut(s)	961, 2127
AccB7I	CCANNNNNTGG	1 cut(s)	1647
AccIII	TCCGGA	1 cut(s)	1600
AciI	CCGC	8 cut(s)	5, 74, 476, 543, 734, 1023, 1197, 1200
AclWI	GGATC	7 cut(s)	193, 516, 593, 767, 1564, 1980, 2024
AcoI	YGGCCR	3 cut(s)	78, 1565, 1985
AcsI	RAATTY	2 cut(s)	133, 515
AcuI	CTGAAG	1 cut(s)	1335
AcyI	GRCGYC	2 cut(s)	233, 2128
AfaI	GTAC	5 cut(s)	145, 963, 1015, 1212, 1876
AfiI	CCNNNNNNNGG	7 cut(s)	49, 50, 764, 1029, 1048, 1647, 2039
AgsI	TTSAA	7 cut(s)	500, 953, 1078, 1224, 1310, 1547, 1612
AjnI	CCWGG	3 cut(s)	181, 1496, 1987
AluBI	AGCT	8 cut(s)	11, 17, 159, 838, 1100, 1163, 1305, 1313
AluI	AGCT	8 cut(s)	11, 17, 159, 838, 1100, 1163, 1305, 1313
Alw21I	GWGCWC	2 cut(s)	1208, 2113
Alw26I	GTCTC	3 cut(s)	95, 129, 685
Alw44I	GTGCAC	2 cut(s)	1204, 2109
AlwI	GGATC	7 cut(s)	193, 516, 593, 767, 1564, 1980, 2024
Ama87I	CYCGRG	3 cut(s)	791, 1112, 1268
Aor13HI	TCCGGA	1 cut(s)	1600
AoxI	GGCC	7 cut(s)	78, 788, 1265, 1565, 1577, 1985, 1998
ApaLI	GTGCAC	2 cut(s)	1204, 2109
ApoI	RAATTY	2 cut(s)	133, 515
ArsI	GACNNNNNNTTYG	2 cut(s)	1504, 1536
Asp718I	GGTACC	1 cut(s)	961
AspLEI	GCGC	1 cut(s)	2130
AspS9I	GGNCC	6 cut(s)	587, 789, 1266, 1578, 1789, 1998
AsuC2I	CCSGG	3 cut(s)	1269, 1270, 1508
AsuII	TTCGAA	2 cut(s)	131, 1708
AsuNHI	GCTAGC	1 cut(s)	1159
AvaI	CYCGRG	3 cut(s)	791, 1112, 1268
AvaII	GGWCC	2 cut(s)	587, 1789
BaeGI	GKGCMC	2 cut(s)	1208, 2113
BanI	GGYRCC	2 cut(s)	961, 2127
BarI	GAAGNNNNNNTAC	2 cut(s)	945, 977
Bbv12I	GWGCWC	2 cut(s)	1208, 2113
BbvI	GCAGC	9 cut(s)	26, 187, 678, 706, 829, 1505, 1735, 1763, 1957
BceAI	ACGGC	4 cut(s)	65, 1307, 1940, 2000
BcgI	CGANNNNNNTGC	2 cut(s)	1504, 1538
BciT130I	CCWGG	3 cut(s)	183, 1498, 1989
BciVI	GTATCC	2 cut(s)	1268, 1452
BclI	TGATCA	1 cut(s)	1909
BcnI	CCSGG	3 cut(s)	1269, 1270, 1508
BcoDI	GTCTC	3 cut(s)	95, 129, 685
BfaI	CTAG	7 cut(s)	971, 1088, 1097, 1160, 1170, 1244, 2027
BfmI	CTRYAG	4 cut(s)	12, 692, 1131, 1749
BfoI	RGCGCY	1 cut(s)	2131
BfuI	GTATCC	2 cut(s)	1268, 1452
BglI	GCCNNNNNGGC	1 cut(s)	1585
BglII	AGATCT	1 cut(s)	967
Bme1390I	CCNGG	6 cut(s)	183, 1269, 1270, 1498, 1508, 1989
Bme18I	GGWCC	2 cut(s)	587, 1789
BmeT110I	CYCGRG	3 cut(s)	791, 1112, 1268
BmgT120I	GGNCC	6 cut(s)	587, 789, 1266, 1578, 1789, 1998
BmiI	GGNNCC	5 cut(s)	588, 963, 1791, 1846, 2129
BmrFI	CCNGG	6 cut(s)	183, 1269, 1270, 1498, 1508, 1989
BmsI	GCATC	4 cut(s)	838, 1246, 1480, 1961
BmtI	GCTAGC	1 cut(s)	1163
BpmI	CTGGAG	2 cut(s)	744, 762
Bpu14I	TTCGAA	2 cut(s)	131, 1708
BpuEI	CTTGAG	4 cut(s)	962, 1086, 1913, 2033
BpuMI	CCSGG	3 cut(s)	1269, 1270, 1508
BsaAI	YACGTR	1 cut(s)	2009
BsaHI	GRCGYC	2 cut(s)	233, 2128
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	3 cut(s)	1214, 1268, 1269
BsaWI	WCCGGW	2 cut(s)	589, 1600
Bsc4I	CCNNNNNNNGG	7 cut(s)	49, 50, 764, 1029, 1048, 1647, 2039
Bse1I	ACTGG	6 cut(s)	779, 907, 1610, 1652, 1700, 1862
BseAI	TCCGGA	1 cut(s)	1600
BseBI	CCWGG	3 cut(s)	183, 1498, 1989
BseDI	CCNNGG	3 cut(s)	1214, 1268, 1269
BseGI	GGATG	5 cut(s)	421, 442, 829, 1016, 1057
BseLI	CCNNNNNNNGG	7 cut(s)	49, 50, 764, 1029, 1048, 1647, 2039
BseMII	CTCAG	3 cut(s)	204, 258, 903
BseNI	ACTGG	6 cut(s)	779, 907, 1610, 1652, 1700, 1862
BseRI	GAGGAG	4 cut(s)	284, 536, 730, 1456
BseSI	GKGCMC	2 cut(s)	1208, 2113
BseXI	GCAGC	9 cut(s)	26, 187, 678, 706, 829, 1505, 1735, 1763, 1957
BshFI	GGCC	7 cut(s)	80, 790, 1267, 1567, 1579, 1987, 2000
BshNI	GGYRCC	2 cut(s)	961, 2127
BsiHKAI	GWGCWC	2 cut(s)	1208, 2113
BsiHKCI	CYCGRG	3 cut(s)	791, 1112, 1268
BsiSI	CCGG	6 cut(s)	590, 1269, 1415, 1508, 1568, 1601
BslFI	GGGAC	3 cut(s)	244, 1490, 1775
BslI	CCNNNNNNNGG	7 cut(s)	49, 50, 764, 1029, 1048, 1647, 2039
BsmAI	GTCTC	3 cut(s)	95, 129, 685
BsmBI	CGTCTC	1 cut(s)	129
BsmFI	GGGAC	3 cut(s)	244, 1490, 1775
BsmI	GAATGC	4 cut(s)	478, 845, 1219, 1704
BsnI	GGCC	7 cut(s)	80, 790, 1267, 1567, 1579, 1987, 2000
Bso31I	GGTCTC	1 cut(s)	95
BsoBI	CYCGRG	3 cut(s)	791, 1112, 1268
Bsp119I	TTCGAA	2 cut(s)	131, 1708
Bsp1286I	GDGCHC	2 cut(s)	1208, 2113
Bsp13I	TCCGGA	1 cut(s)	1600
Bsp1407I	TGTACA	1 cut(s)	1013
BspACI	CCGC	8 cut(s)	5, 74, 476, 543, 734, 1023, 1197, 1200
BspANI	GGCC	7 cut(s)	80, 790, 1267, 1567, 1579, 1987, 2000
BspCNI	CTCAG	3 cut(s)	205, 259, 904
BspEI	TCCGGA	1 cut(s)	1600
BspHI	TCATGA	1 cut(s)	253
BspLI	GGNNCC	5 cut(s)	588, 963, 1791, 1846, 2129
BspMAI	CTGCAG	3 cut(s)	16, 696, 1753
BspOI	GCTAGC	1 cut(s)	1163
BspPI	GGATC	7 cut(s)	193, 516, 593, 767, 1564, 1980, 2024
BspQI	GCTCTTC	2 cut(s)	393, 1542
BspT104I	TTCGAA	2 cut(s)	131, 1708
BspT107I	GGYRCC	2 cut(s)	961, 2127
BspTNI	GGTCTC	1 cut(s)	95
BsrGI	TGTACA	1 cut(s)	1013
BsrI	ACTGG	6 cut(s)	779, 907, 1610, 1652, 1700, 1862
BssECI	CCNNGG	3 cut(s)	1214, 1268, 1269
BssNI	GRCGYC	2 cut(s)	233, 2128
BssT1I	CCWWGG	1 cut(s)	1214
Bst2UI	CCWGG	3 cut(s)	183, 1498, 1989
Bst4CI	ACNGT	2 cut(s)	1132, 1210
Bst6I	CTCTTC	6 cut(s)	393, 551, 563, 759, 948, 1542
BstACI	GRCGYC	2 cut(s)	233, 2128
BstAUI	TGTACA	1 cut(s)	1013
BstBAI	YACGTR	1 cut(s)	2009
BstBI	TTCGAA	2 cut(s)	131, 1708
BstC8I	GCNNGC	5 cut(s)	644, 840, 1161, 1905, 1950
BstDEI	CTNAG	3 cut(s)	213, 267, 912
BstENI	CCTNNNNNAGG	1 cut(s)	1046
BstF5I	GGATG	5 cut(s)	421, 442, 829, 1016, 1057
BstH2I	RGCGCY	1 cut(s)	2131
BstHHI	GCGC	1 cut(s)	2130
BstMAI	GTCTC	3 cut(s)	95, 129, 685
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 639, 703, 835, 1031, 1203, 1319, 1585
BstNI	CCWGG	3 cut(s)	183, 1498, 1989
BstNSI	RCATGY	1 cut(s)	1907
BstSCI	CCNGG	6 cut(s)	181, 1267, 1268, 1496, 1506, 1987
BstSFI	CTRYAG	4 cut(s)	12, 692, 1131, 1749
BstSLI	GKGCMC	2 cut(s)	1208, 2113
BstV1I	GCAGC	9 cut(s)	26, 187, 678, 706, 829, 1505, 1735, 1763, 1957
BstX2I	RGATCY	2 cut(s)	967, 2029
BstXI	CCANNNNNNTGG	2 cut(s)	786, 1995
BstYI	RGATCY	2 cut(s)	967, 2029
BsuI	GTATCC	2 cut(s)	1268, 1452
BsuRI	GGCC	7 cut(s)	80, 790, 1267, 1567, 1579, 1987, 2000
BtsCI	GGATG	5 cut(s)	421, 442, 829, 1016, 1057
BtsI	GCAGTG	1 cut(s)	1192
BtsIMutI	CAGTG	5 cut(s)	144, 1192, 1206, 1390, 1645
Cac8I	GCNNGC	5 cut(s)	644, 840, 1161, 1905, 1950
CciI	TCATGA	1 cut(s)	253
CfoI	GCGC	1 cut(s)	2130
Cfr13I	GGNCC	6 cut(s)	587, 789, 1266, 1578, 1789, 1998
Cfr9I	CCCGGG	1 cut(s)	1268
Csp6I	GTAC	5 cut(s)	144, 962, 1014, 1211, 1875
CviAII	CATG	7 cut(s)	254, 627, 1298, 1723, 1799, 1904, 2124
CviQI	GTAC	5 cut(s)	144, 962, 1014, 1211, 1875
DdeI	CTNAG	3 cut(s)	213, 267, 912
DinI	GGCGCC	1 cut(s)	2129
EaeI	YGGCCR	3 cut(s)	78, 1565, 1985
Eam1104I	CTCTTC	6 cut(s)	393, 551, 563, 759, 948, 1542
EarI	CTCTTC	6 cut(s)	393, 551, 563, 759, 948, 1542
Eco130I	CCWWGG	1 cut(s)	1214
Eco31I	GGTCTC	1 cut(s)	95
Eco32I	GATATC	2 cut(s)	1006, 1598
Eco47I	GGWCC	2 cut(s)	587, 1789
Eco57I	CTGAAG	1 cut(s)	1335
Eco88I	CYCGRG	3 cut(s)	791, 1112, 1268
EcoNI	CCTNNNNNAGG	1 cut(s)	1046
EcoRI	GAATTC	1 cut(s)	133
EcoRII	CCWGG	3 cut(s)	181, 1496, 1987
EcoRV	GATATC	2 cut(s)	1006, 1598
EcoT14I	CCWWGG	1 cut(s)	1214
EgeI	GGCGCC	1 cut(s)	2129
EheI	GGCGCC	1 cut(s)	2129
ErhI	CCWWGG	1 cut(s)	1214
Esp3I	CGTCTC	1 cut(s)	129
FaeI	CATG	7 cut(s)	257, 630, 1301, 1726, 1802, 1907, 2127
FalI	AAGNNNNNCTT	4 cut(s)	1147, 1179, 2062, 2094
FaqI	GGGAC	3 cut(s)	244, 1490, 1775
FatI	CATG	7 cut(s)	253, 626, 1297, 1722, 1798, 1903, 2123
FauI	CCCGC	1 cut(s)	1193
FauNDI	CATATG	1 cut(s)	1891
FbaI	TGATCA	1 cut(s)	1909
FokI	GGATG	5 cut(s)	428, 449, 816, 1023, 1064
FspBI	CTAG	7 cut(s)	971, 1088, 1097, 1160, 1170, 1244, 2027
GlaI	GCGC	1 cut(s)	2129
GsuI	CTGGAG	2 cut(s)	744, 762
HaeII	RGCGCY	1 cut(s)	2131
HaeIII	GGCC	7 cut(s)	80, 790, 1267, 1567, 1579, 1987, 2000
HapII	CCGG	6 cut(s)	590, 1269, 1415, 1508, 1568, 1601
HhaI	GCGC	1 cut(s)	2130
Hin1I	GRCGYC	2 cut(s)	233, 2128
Hin1II	CATG	7 cut(s)	257, 630, 1301, 1726, 1802, 1907, 2127
Hin6I	GCGC	1 cut(s)	2128
HinP1I	GCGC	1 cut(s)	2128
HincII	GTYRAC	3 cut(s)	1093, 1290, 1663
HindII	GTYRAC	3 cut(s)	1093, 1290, 1663
HindIII	AAGCTT	1 cut(s)	836
HinfI	GANTC	7 cut(s)	548, 875, 944, 1019, 1418, 1865, 2023
HpaII	CCGG	6 cut(s)	590, 1269, 1415, 1508, 1568, 1601
Hpy166II	GTNNAC	8 cut(s)	111, 120, 725, 1093, 1206, 1290, 1663, 2111
Hpy188I	TCNGA	8 cut(s)	214, 268, 553, 603, 913, 1010, 1148, 1561
Hpy8I	GTNNAC	8 cut(s)	111, 120, 725, 1093, 1206, 1290, 1663, 2111
HpyCH4III	ACNGT	2 cut(s)	1132, 1210
HpyCH4IV	ACGT	4 cut(s)	122, 233, 822, 2008
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 639, 703, 835, 1031, 1203, 1319, 1585
HpyF3I	CTNAG	3 cut(s)	213, 267, 912
HpySE526I	ACGT	4 cut(s)	122, 233, 822, 2008
Hsp92I	GRCGYC	2 cut(s)	233, 2128
Hsp92II	CATG	7 cut(s)	257, 630, 1301, 1726, 1802, 1907, 2127
HspAI	GCGC	1 cut(s)	2128
KasI	GGCGCC	1 cut(s)	2127
Kpn2I	TCCGGA	1 cut(s)	1600
KpnI	GGTACC	1 cut(s)	965
Ksp22I	TGATCA	1 cut(s)	1909
LguI	GCTCTTC	2 cut(s)	393, 1542
LmnI	GCTCC	3 cut(s)	192, 639, 826
Lsp1109I	GCAGC	9 cut(s)	26, 187, 678, 706, 829, 1505, 1735, 1763, 1957
LweI	GCATC	4 cut(s)	838, 1246, 1480, 1961
MaeI	CTAG	7 cut(s)	971, 1088, 1097, 1160, 1170, 1244, 2027
MaeII	ACGT	4 cut(s)	122, 233, 822, 2008
MaeIII	GTNAC	3 cut(s)	324, 1510, 2009
MfeI	CAATTG	2 cut(s)	353, 1956
MflI	RGATCY	2 cut(s)	967, 2029
MhlI	GDGCHC	2 cut(s)	1208, 2113
MluCI	AATT	5 cut(s)	133, 353, 515, 649, 1956
Mly113I	GGCGCC	1 cut(s)	2128
MlyI	GAGTC	4 cut(s)	884, 953, 1412, 1874
MmeI	TCCRAC	3 cut(s)	510, 1822, 2016
MroI	TCCGGA	1 cut(s)	1600
MseI	TTAA	6 cut(s)	261, 315, 582, 918, 1058, 1262
MslI	CAYNNNNRTG	1 cut(s)	1224
MspA1I	CMGCKG	1 cut(s)	74
MspI	CCGG	6 cut(s)	590, 1269, 1415, 1508, 1568, 1601
MspR9I	CCNGG	6 cut(s)	183, 1269, 1270, 1498, 1508, 1989
MunI	CAATTG	2 cut(s)	353, 1956
Mva1269I	GAATGC	4 cut(s)	478, 845, 1219, 1704
MvaI	CCWGG	3 cut(s)	183, 1498, 1989
MwoI	GCNNNNNNNGC	8 cut(s)	11, 639, 703, 835, 1031, 1203, 1319, 1585
NarI	GGCGCC	1 cut(s)	2128
NciI	CCSGG	3 cut(s)	1269, 1270, 1508
NdeI	CATATG	1 cut(s)	1891
NheI	GCTAGC	1 cut(s)	1159
NlaIII	CATG	7 cut(s)	257, 630, 1301, 1726, 1802, 1907, 2127
NlaIV	GGNNCC	5 cut(s)	588, 963, 1791, 1846, 2129
NmuCI	GTSAC	2 cut(s)	1510, 2009
NspI	RCATGY	1 cut(s)	1907
NspV	TTCGAA	2 cut(s)	131, 1708
PaeI	GCATGC	1 cut(s)	1907
PaeR7I	CTCGAG	1 cut(s)	1112
PagI	TCATGA	1 cut(s)	253
PciSI	GCTCTTC	2 cut(s)	393, 1542
PcsI	WCGNNNNNNNCGW	1 cut(s)	128
PctI	GAATGC	4 cut(s)	478, 845, 1219, 1704
PfeI	GAWTC	3 cut(s)	548, 1019, 2023
PflMI	CCANNNNNTGG	1 cut(s)	1647
PfoI	TCCNGGA	1 cut(s)	181
PleI	GAGTC	4 cut(s)	883, 952, 1412, 1873
PluTI	GGCGCC	1 cut(s)	2131
PpsI	GAGTC	4 cut(s)	883, 952, 1412, 1873
Ppu21I	YACGTR	1 cut(s)	2009
Psp6I	CCWGG	3 cut(s)	181, 1496, 1987
PspGI	CCWGG	3 cut(s)	181, 1496, 1987
PspN4I	GGNNCC	5 cut(s)	588, 963, 1791, 1846, 2129
PspPI	GGNCC	6 cut(s)	587, 789, 1266, 1578, 1789, 1998
PspXI	VCTCGAGB	1 cut(s)	1112
PstI	CTGCAG	3 cut(s)	16, 696, 1753
PsuI	RGATCY	2 cut(s)	967, 2029
RsaI	GTAC	5 cut(s)	145, 963, 1015, 1212, 1876
RsaNI	GTAC	5 cut(s)	144, 962, 1014, 1211, 1875
RseI	CAYNNNNRTG	1 cut(s)	1224
SapI	GCTCTTC	2 cut(s)	393, 1542
SaqAI	TTAA	6 cut(s)	261, 315, 582, 918, 1058, 1262
Sau96I	GGNCC	6 cut(s)	587, 789, 1266, 1578, 1789, 1998
SchI	GAGTC	4 cut(s)	884, 953, 1412, 1874
ScrFI	CCNGG	6 cut(s)	183, 1269, 1270, 1498, 1508, 1989
SduI	GDGCHC	2 cut(s)	1208, 2113
SfaNI	GCATC	4 cut(s)	838, 1246, 1480, 1961
SfcI	CTRYAG	4 cut(s)	12, 692, 1131, 1749
SfoI	GGCGCC	1 cut(s)	2129
Sfr274I	CTCGAG	1 cut(s)	1112
SfuI	TTCGAA	2 cut(s)	131, 1708
SinI	GGWCC	2 cut(s)	587, 1789
SlaI	CTCGAG	1 cut(s)	1112
SmaI	CCCGGG	1 cut(s)	1270
SmiMI	CAYNNNNRTG	1 cut(s)	1224
SmlI	CTYRAG	5 cut(s)	941, 1101, 1112, 1928, 2048
SmoI	CTYRAG	5 cut(s)	941, 1101, 1112, 1928, 2048
SphI	GCATGC	1 cut(s)	1907
Sse9I	AATT	5 cut(s)	133, 353, 515, 649, 1956
SsiI	CCGC	8 cut(s)	5, 74, 476, 543, 734, 1023, 1197, 1200
SspDI	GGCGCC	1 cut(s)	2127
SspI	AATATT	1 cut(s)	1357
SspMI	CTAG	7 cut(s)	971, 1088, 1097, 1160, 1170, 1244, 2027
StyD4I	CCNGG	6 cut(s)	181, 1267, 1268, 1496, 1506, 1987
StyI	CCWWGG	1 cut(s)	1214
TaaI	ACNGT	2 cut(s)	1132, 1210
TaiI	ACGT	4 cut(s)	125, 236, 825, 2011
TaqI	TCGA	5 cut(s)	131, 341, 1113, 1590, 1708
TasI	AATT	5 cut(s)	133, 353, 515, 649, 1956
TatI	WGTACW	2 cut(s)	143, 1013
TauI	GCSGC	1 cut(s)	1200
TfiI	GAWTC	3 cut(s)	548, 1019, 2023
Tru1I	TTAA	6 cut(s)	261, 315, 582, 918, 1058, 1262
Tru9I	TTAA	6 cut(s)	261, 315, 582, 918, 1058, 1262
TscAI	CASTG	5 cut(s)	144, 1199, 1213, 1390, 1652
TseFI	GTSAC	2 cut(s)	1510, 2009
Tsp45I	GTSAC	2 cut(s)	1510, 2009
TspDTI	ATGAA	8 cut(s)	405, 411, 462, 1265, 1392, 1739, 1796, 1810
TspGWI	ACGGA	2 cut(s)	980, 2022
TspMI	CCCGGG	1 cut(s)	1268
TspRI	CASTG	5 cut(s)	144, 1199, 1213, 1390, 1652
Van91I	CCANNNNNTGG	1 cut(s)	1647
VneI	GTGCAC	2 cut(s)	1204, 2109
VpaK11BI	GGWCC	2 cut(s)	587, 1789
XagI	CCTNNNNNAGG	1 cut(s)	1046
XapI	RAATTY	2 cut(s)	133, 515
XbaI	TCTAGA	3 cut(s)	970, 1243, 2026
XceI	RCATGY	1 cut(s)	1907
XcmI	CCANNNNNNNNNTGG	3 cut(s)	955, 972, 1494
XhoI	CTCGAG	1 cut(s)	1112
XmaI	CCCGGG	1 cut(s)	1268
XspI	CTAG	7 cut(s)	971, 1088, 1097, 1160, 1170, 1244, 2027
ZraI	GACGTC	1 cut(s)	234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.