MD08G1039400.v1.1

Transmembrane protein 147-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016163
Physical Location & Seq
Forward (+)
2897976 .. 2900263
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1039400.v1.1.491

Sequence Viewer

Length: 687 bp
ATGACGGTGTTTCACTTCTTCAACTGCGCAATTCTGACGTTCGGCCCTCACGCCGTCTACTACTCCGCCACTCCGCTATCGGAGTACGATACGCTCGGGACTTCCGTCAAAGCAGCTCTGGTTTATCTCGGTACAGCTTTAGTGAAGCTGGTTTGTTTGGCAACTTTTCTGAACGTATCTGACAGTGAGAGCTTCGATCCGTATCAGGAATTGTTGAAAGCTCTGATCGGTTTTATAGACGTTGCTGGACTTTACTTCGCATTGACCCAGTTGACACACAGGAATATCTCTCAGAATCATAAATTTCAGGCAGTGGGACTTGGATGGGCTTTTGCTGATTCTGTTCTCCATAGACTGGCACCGCTGTGGTTGGGCGCTAGAGGACTAGAATTCACTTGGGATTACATTCTCCAAGGCCTTGAAGCAAATGCCAATCTGGTCTTGAGTATATCTCTTGCCGCATTTGGATCTTTGATATGGCTTCGGAAAAACAAGCCTAGGACGTTGGTTCCTTTGATATACATATGTGCAGGATTGGTGGCAACCATGCCATCGATCACAAGCTATCTGACGCGAGGCTGGGGTTGGCACTTTCTGGACGTGGTAATGTTTGAACTCGGTACCTCTCTAGTGATGGCTCTCATCAGTTGGTGGCTTGTTGCTCAATGTCAGAGACCCTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2053

Protein Analysis

229

Amino Acids

25.3

Weight (kDa)

7.0

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2053 PF09767 2 - 161 3.7e-59 Predicted membrane protein (DUF2053)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47640
fragaria_vesca FvH4_2g40630
malus_domestica MD08G1039400.v1.1 MD15G1034100.v1.1
prunus_persica Prupe.1G387900_v2.0.a1
pyrus_communis pycom08g03150 pycom15g03140
rosa_chinensis RchiOBHm_Chr6g0300041
rosa_laevigata RLG00000011370
rosa_multiflora Rmu_sc0010665.1_g000003
rosa_roxburghii Rroxscaffold_7G00168170
rosa_rugosa Rorug06G0293800
rosa_samantha Rh6BG413700 Rh6DG406400
rosa_wichuraiana Rw6G035480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 28
Acc65I GGTACC 1 cut(s) 620
AccB1I GGYRCC 2 cut(s) 358, 620
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 1 cut(s) 57
AccII CGCG 1 cut(s) 574
AciI CCGC 4 cut(s) 66, 74, 362, 459
AclWI GGATC 2 cut(s) 191, 475
AcsI RAATTY 2 cut(s) 302, 389
AfaI GTAC 3 cut(s) 86, 133, 622
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 4 cut(s) 22, 217, 422, 614
AjiI CACGTC 1 cut(s) 601
AleI CACNNNNGTG 1 cut(s) 364
AluBI AGCT 6 cut(s) 116, 137, 148, 192, 221, 564
AluI AGCT 6 cut(s) 116, 137, 148, 192, 221, 564
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 2 cut(s) 191, 475
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 2 cut(s) 43, 415
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 2 cut(s) 302, 389
Asp718I GGTACC 1 cut(s) 620
AspA2I CCTAGG 1 cut(s) 497
AspLEI GCGC 2 cut(s) 29, 377
AspS9I GGNCC 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 95
AvrII CCTAGG 1 cut(s) 497
BanI GGYRCC 2 cut(s) 358, 620
BbvI GCAGC 1 cut(s) 125
BccI CCATC 3 cut(s) 318, 559, 628
BceAI ACGGC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 4 cut(s) 378, 386, 498, 629
BfoI RGCGCY 1 cut(s) 378
BisI GCNGC 2 cut(s) 114, 459
BlnI CCTAGG 1 cut(s) 497
BlsI GCNGC 2 cut(s) 115, 460
BmeT110I CYCGRG 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 3 cut(s) 360, 510, 622
BmrI ACTGGG 1 cut(s) 262
BmuI ACTGGG 1 cut(s) 262
BoxI GACNNNNGTC 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpuEI CTTGAG 1 cut(s) 463
Bsa29I ATCGAT 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 667
BsaJI CCNNGG 2 cut(s) 412, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 268, 360
Bse8I GATNNNNATC 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 554
BseDI CCNNGG 2 cut(s) 412, 497
BseGI GGATG 1 cut(s) 329
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 2 cut(s) 268, 360
BseXI GCAGC 1 cut(s) 125
BseYI CCCAGC 1 cut(s) 579
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 45, 417
BshNI GGYRCC 2 cut(s) 358, 620
BshVI ATCGAT 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 95
BslFI GGGAC 2 cut(s) 112, 330
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 112, 330
BsnI GGCC 2 cut(s) 45, 417
Bso31I GGTCTC 1 cut(s) 667
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 4 cut(s) 196, 225, 467, 555
BspACI CCGC 4 cut(s) 66, 74, 362, 459
BspANI GGCC 2 cut(s) 45, 417
BspCNI CTCAG 1 cut(s) 304
BspDI ATCGAT 1 cut(s) 554
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 360, 510, 622
BspPI GGATC 2 cut(s) 191, 475
BspT107I GGYRCC 2 cut(s) 358, 620
BspTNI GGTCTC 1 cut(s) 667
BsrI ACTGG 2 cut(s) 268, 360
BssECI CCNNGG 2 cut(s) 412, 497
BssMI GATC 4 cut(s) 196, 225, 467, 555
BssT1I CCWWGG 2 cut(s) 412, 497
Bst4CI ACNGT 2 cut(s) 7, 185
BstDEI CTNAG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 329
BstFNI CGCG 1 cut(s) 574
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 2 cut(s) 29, 377
BstKTI GATC 4 cut(s) 199, 228, 470, 558
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 4 cut(s) 196, 225, 467, 555
BstPAI GACNNNNGTC 1 cut(s) 104
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu15I ATCGAT 1 cut(s) 554
BsuRI GGCC 2 cut(s) 45, 417
BsuTUI ATCGAT 1 cut(s) 554
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 1 cut(s) 329
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 190, 318
CfoI GCGC 2 cut(s) 29, 377
Cfr13I GGNCC 1 cut(s) 44
ClaI ATCGAT 1 cut(s) 554
CseI GACGC 1 cut(s) 580
Csp6I GTAC 3 cut(s) 85, 132, 621
CviAII CATG 1 cut(s) 547
CviQI GTAC 3 cut(s) 85, 132, 621
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 198, 227, 469, 557
DpnII GATC 4 cut(s) 196, 225, 467, 555
EciI GGCGGA 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 412, 497
Eco147I AGGCCT 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 667
Eco88I CYCGRG 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 389
EcoT14I CCWWGG 2 cut(s) 412, 497
ErhI CCWWGG 2 cut(s) 412, 497
FaeI CATG 1 cut(s) 550
FaiI YATR 9 cut(s) 236, 300, 351, 449, 478, 520, 524, 526, 548
FaqI GGGAC 2 cut(s) 112, 330
FatI CATG 1 cut(s) 546
FauNDI CATATG 1 cut(s) 524
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 114, 459
FokI GGATG 1 cut(s) 336
Fsp4HI GCNGC 2 cut(s) 114, 459
FspBI CTAG 4 cut(s) 378, 386, 498, 629
FspI TGCGCA 1 cut(s) 28
GlaI GCGC 2 cut(s) 28, 376
GluI GCNGC 2 cut(s) 114, 459
GsaI CCCAGC 1 cut(s) 583
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 2 cut(s) 45, 417
HgaI GACGC 1 cut(s) 580
HhaI GCGC 2 cut(s) 29, 377
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 2 cut(s) 27, 375
HinP1I GCGC 2 cut(s) 27, 375
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 2 cut(s) 295, 338
Hpy166II GTNNAC 2 cut(s) 58, 273
Hpy188I TCNGA 9 cut(s) 36, 82, 171, 181, 225, 294, 486, 570, 672
Hpy188III TCNNGA 4 cut(s) 97, 206, 442, 596
Hpy8I GTNNAC 2 cut(s) 58, 273
HpyCH4III ACNGT 2 cut(s) 7, 185
HpyCH4IV ACGT 5 cut(s) 38, 174, 240, 503, 600
HpyCH4V TGCA 1 cut(s) 530
HpyF3I CTNAG 1 cut(s) 291
HpySE526I ACGT 5 cut(s) 38, 174, 240, 503, 600
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 2 cut(s) 27, 375
KpnI GGTACC 1 cut(s) 624
Kzo9I GATC 4 cut(s) 196, 225, 467, 555
Lsp1109I GCAGC 1 cut(s) 125
MaeI CTAG 4 cut(s) 378, 386, 498, 629
MaeII ACGT 5 cut(s) 38, 174, 240, 503, 600
MalI GATC 4 cut(s) 198, 227, 469, 557
MboI GATC 4 cut(s) 196, 225, 467, 555
MboII GAAGA 1 cut(s) 10
MflI RGATCY 1 cut(s) 467
MluCI AATT 4 cut(s) 30, 209, 302, 389
MnlI CCTC 4 cut(s) 57, 374, 569, 634
MslI CAYNNNNRTG 1 cut(s) 364
MspA1I CMGCKG 1 cut(s) 364
MvnI CGCG 1 cut(s) 574
NdeI CATATG 1 cut(s) 524
NdeII GATC 4 cut(s) 196, 225, 467, 555
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 3 cut(s) 360, 510, 622
NsbI TGCGCA 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 364
PceI AGGCCT 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 2 cut(s) 295, 338
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 2 cut(s) 115, 460
PshAI GACNNNNGTC 1 cut(s) 104
PspFI CCCAGC 1 cut(s) 579
PspN4I GGNNCC 3 cut(s) 360, 510, 622
PspPI GGNCC 1 cut(s) 44
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 3 cut(s) 86, 133, 622
RsaNI GTAC 3 cut(s) 85, 132, 621
RseI CAYNNNNRTG 1 cut(s) 364
SatI GCNGC 2 cut(s) 114, 459
Sau3AI GATC 4 cut(s) 196, 225, 467, 555
Sau96I GGNCC 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 364
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 4 cut(s) 30, 209, 302, 389
SseBI AGGCCT 1 cut(s) 417
SsiI CCGC 4 cut(s) 66, 74, 362, 459
SspMI CTAG 4 cut(s) 378, 386, 498, 629
StuI AGGCCT 1 cut(s) 417
StyI CCWWGG 2 cut(s) 412, 497
TaaI ACNGT 2 cut(s) 7, 185
TaiI ACGT 5 cut(s) 41, 177, 243, 506, 603
TaqI TCGA 2 cut(s) 195, 554
TasI AATT 4 cut(s) 30, 209, 302, 389
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 2 cut(s) 295, 338
TscAI CASTG 2 cut(s) 190, 318
TseI GCWGC 1 cut(s) 113
TspGWI ACGGA 2 cut(s) 94, 189
TspRI CASTG 2 cut(s) 190, 318
Van91I CCANNNNNTGG 1 cut(s) 355
XapI RAATTY 2 cut(s) 302, 389
XmaJI CCTAGG 1 cut(s) 497
XmiI GTMKAC 1 cut(s) 57
XspI CTAG 4 cut(s) 378, 386, 498, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.