MD08G1061000.v1.1

Protein REVERSION-TO-ETHYLENE SENSITIVITY1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004008
Physical Location & Seq
Reverse (-)
4829876 .. 4831789
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1061000.v1.1.491

Sequence Viewer

Length: 732 bp
ATGCGGCACGTTAGATTTCCTATGATGGAGCTGAAAGAAGCGTATGACATTGAGCATTTGACCTCTACGCAGAATATTGAGCATGAGCTGTGGCCTCTCACTGAAATTGATCCGAAGAAGGCAAAGTTTCCCTGCTGTATAGTTTGGACTCCTCTCCCGGTAGTCTCCTGGTTGGCACCATTCATCGGACATGTTGGCATTTGCAGGGAGGATGGAGCTATTTTAGATTTTTCTGGTTCCAATTTTGTGAATGTTGATGACTTTGCATTTGGTGCCGTTGCCAGATATCTCCAAATTGATCGAAAACAGTGTTGTTTTGCCGCAAATCTGGGTAGCCACACTTGCAAGCATGGGTACAATCATTCAGAGTTTGGGACTGCATTAACGTGGGATGATGCCTTGCAGTCAAGTTCGCGGTACTTTGAACACAAAACCTACAACCTCTTCACTTGCAACAGCCATTCATTTGTAGCGAACTGTCTCAATCGGCTGTGCTATGGCGGATCAATGAGTTGGAACATGATCAACGTGGCAGGCTTAGTACTGCTCAAGGGGCATTGGGTTGGTGCCACATCCGTCTTGAGGTCATTCCTCCCTTTTGTATTGGTGCTCGCTCTGGGTGTTGCCATGGTCGGATGGCTATTCGTTGTTGCGCTTTTCTCCTTCTCGCTCCTCCTTCTGGTATGGTTTATACTGGGCAGTTATTGTTTCAAGACCTTGCTGGAGTGCTAG

Protein Analysis

244

Amino Acids

27.44

Weight (kDa)

6.53

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 37 - 185 1.2e-72 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 175, 272, 566
AccII CGCG 1 cut(s) 415
AciI CCGC 4 cut(s) 4, 321, 415, 501
AclWI GGATC 2 cut(s) 104, 511
AfaI GTAC 3 cut(s) 356, 419, 543
AfiI CCNNNNNNNGG 3 cut(s) 185, 582, 679
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 2 cut(s) 425, 712
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 3 cut(s) 31, 88, 218
AluI AGCT 3 cut(s) 31, 88, 218
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 2 cut(s) 169, 485
AlwI GGATC 2 cut(s) 104, 511
AoxI GGCC 1 cut(s) 92
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AspLEI GCGC 1 cut(s) 655
AsuC2I CCSGG 1 cut(s) 158
BanI GGYRCC 3 cut(s) 175, 272, 566
Bbv12I GWGCWC 1 cut(s) 612
BccI CCATC 3 cut(s) 19, 206, 630
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 169
BclI TGATCA 1 cut(s) 522
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 2 cut(s) 169, 485
BfaI CTAG 1 cut(s) 730
BisI GCNGC 2 cut(s) 5, 321
BlsI GCNGC 2 cut(s) 6, 322
BmcAI AGTACT 1 cut(s) 543
Bme1390I CCNGG 2 cut(s) 158, 169
BmiI GGNNCC 4 cut(s) 177, 238, 274, 568
BmrFI CCNGG 2 cut(s) 158, 169
BmrI ACTGGG 1 cut(s) 704
BmsI GCATC 1 cut(s) 385
BmuI ACTGGG 1 cut(s) 704
BpuEI CTTGAG 2 cut(s) 533, 601
BpuMI CCSGG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 627
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 582, 679
Bse1I ACTGG 1 cut(s) 699
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 4 cut(s) 217, 397, 572, 641
BseLI CCNNNNNNNGG 3 cut(s) 185, 582, 679
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 2 cut(s) 141, 662
Bsh1236I CGCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 3 cut(s) 175, 272, 566
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 3 cut(s) 185, 582, 679
BsmAI GTCTC 2 cut(s) 169, 485
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 612
Bsp143I GATC 4 cut(s) 109, 298, 503, 522
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 4 cut(s) 4, 321, 415, 501
BspANI GGCC 1 cut(s) 94
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 4 cut(s) 177, 238, 274, 568
BspPI GGATC 2 cut(s) 104, 511
BspT107I GGYRCC 3 cut(s) 175, 272, 566
BsrI ACTGG 1 cut(s) 699
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 4 cut(s) 109, 298, 503, 522
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 309, 479
Bst6I CTCTTC 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 3 cut(s) 347, 535, 612
BstDEI CTNAG 1 cut(s) 538
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 4 cut(s) 217, 397, 572, 641
BstFNI CGCG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 4 cut(s) 112, 301, 506, 525
BstMAI GTCTC 2 cut(s) 169, 485
BstMBI GATC 4 cut(s) 109, 298, 503, 522
BstMWI GCNNNNNNNGC 3 cut(s) 272, 342, 553
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 2 cut(s) 156, 167
BstUI CGCG 1 cut(s) 415
BsuRI GGCC 1 cut(s) 94
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 217, 397, 572, 641
BtsIMutI CAGTG 2 cut(s) 99, 314
Cac8I GCNNGC 3 cut(s) 347, 535, 612
CfoI GCGC 1 cut(s) 655
Csp6I GTAC 3 cut(s) 355, 418, 542
CviAII CATG 5 cut(s) 83, 191, 350, 520, 628
CviJI RGCY 9 cut(s) 31, 88, 94, 218, 336, 459, 490, 537, 640
CviKI_1 RGCY 9 cut(s) 31, 88, 94, 218, 336, 459, 490, 537, 640
CviQI GTAC 3 cut(s) 355, 418, 542
DdeI CTNAG 1 cut(s) 538
DpnI GATC 4 cut(s) 111, 300, 505, 524
DpnII GATC 4 cut(s) 109, 298, 503, 522
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
EciI GGCGGA 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 627
Eco32I GATATC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 167
EcoRV GATATC 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 5 cut(s) 86, 194, 353, 523, 631
FaqI GGGAC 1 cut(s) 388
FatI CATG 5 cut(s) 82, 190, 349, 519, 627
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 5, 321
FokI GGATG 4 cut(s) 224, 404, 559, 648
Fsp4HI GCNGC 2 cut(s) 5, 321
FspBI CTAG 1 cut(s) 730
GlaI GCGC 1 cut(s) 654
GluI GCNGC 2 cut(s) 5, 321
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 158
HhaI GCGC 1 cut(s) 655
Hin1II CATG 5 cut(s) 86, 194, 353, 523, 631
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 158
Hpy188I TCNGA 4 cut(s) 114, 188, 367, 635
Hpy188III TCNNGA 2 cut(s) 580, 712
HpyAV CCTTC 3 cut(s) 112, 673, 686
HpyCH4III ACNGT 2 cut(s) 309, 479
HpyCH4IV ACGT 3 cut(s) 9, 386, 528
HpyCH4V TGCA 6 cut(s) 204, 266, 345, 380, 403, 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 342, 553
HpyF3I CTNAG 1 cut(s) 538
HpySE526I ACGT 3 cut(s) 9, 386, 528
Hsp92II CATG 5 cut(s) 86, 194, 353, 523, 631
HspAI GCGC 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 4 cut(s) 109, 298, 503, 522
LmnI GCTCC 3 cut(s) 28, 215, 675
LweI GCATC 1 cut(s) 385
MaeI CTAG 1 cut(s) 730
MaeII ACGT 3 cut(s) 9, 386, 528
MalI GATC 4 cut(s) 111, 300, 505, 524
MboI GATC 4 cut(s) 109, 298, 503, 522
MboII GAAGA 2 cut(s) 127, 436
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 3 cut(s) 105, 241, 294
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 2 cut(s) 494, 613
MnlI CCTC 8 cut(s) 73, 105, 162, 202, 452, 576, 602, 683
MseI TTAA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 385
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 2 cut(s) 158, 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 3 cut(s) 272, 342, 553
NciI CCSGG 1 cut(s) 158
NcoI CCATGG 1 cut(s) 627
NdeII GATC 4 cut(s) 109, 298, 503, 522
NlaIII CATG 5 cut(s) 86, 194, 353, 523, 631
NlaIV GGNNCC 4 cut(s) 177, 238, 274, 568
NspI RCATGY 1 cut(s) 194
PciI ACATGT 1 cut(s) 190
PkrI GCNGC 2 cut(s) 6, 322
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PscI ACATGT 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 4 cut(s) 177, 238, 274, 568
RsaI GTAC 3 cut(s) 356, 419, 543
RsaNI GTAC 3 cut(s) 355, 418, 542
RseI CAYNNNNRTG 1 cut(s) 385
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 2 cut(s) 5, 321
Sau3AI GATC 4 cut(s) 109, 298, 503, 522
ScaI AGTACT 1 cut(s) 543
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 2 cut(s) 158, 169
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 385
SmlI CTYRAG 2 cut(s) 548, 580
SmoI CTYRAG 2 cut(s) 548, 580
Sse9I AATT 3 cut(s) 105, 241, 294
SsiI CCGC 4 cut(s) 4, 321, 415, 501
SspI AATATT 1 cut(s) 76
SspMI CTAG 1 cut(s) 730
StyD4I CCNGG 2 cut(s) 156, 167
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 2 cut(s) 309, 479
TaiI ACGT 3 cut(s) 12, 389, 531
TaqI TCGA 1 cut(s) 301
TasI AATT 3 cut(s) 105, 241, 294
TatI WGTACW 1 cut(s) 541
TauI GCSGC 2 cut(s) 7, 323
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 106, 314
TspDTI ATGAA 2 cut(s) 172, 453
TspGWI ACGGA 1 cut(s) 565
TspRI CASTG 2 cut(s) 106, 314
XceI RCATGY 1 cut(s) 194
XspI CTAG 1 cut(s) 730
ZrmI AGTACT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.