MD15G1060400.v1.1

Protein REVERSION-TO-ETHYLENE SENSITIVITY1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004008
Physical Location & Seq
Forward (+)
4059785 .. 4061829
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1060400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGATGGAGCTAAAGGAAGCTTATGACATCGAGCGTATGTCCTCTACGCAGAATATTCAGCATGAGCTGTGGCCACTCGATGAAATTGATCCGAAGAAGGCAAAGTTTCCTTGCTGTATAGTTTGGACTCCGCTCCCCGTAGTCTCTTGGTTGGCACCATTCATCGGACATGTTGGCATTTGCAGGGAGGATGGAGCTATTTTAGATTTTTCTGGTTCCAATTTTGTGAATGTTGATGATTTCACATTTGGTCCCGTAGCCAGATATCTCCAAATTGATAGAAAACAGTGTTGTTTTGCCCCAAATCTGGGTGGCCACACTTGCAAGCATGGGTATGAGCATTCAGAGTTTGGGACAGCAATAACGTGGGATGATGCCTTGCGGTCAAGTTCGCGGTACTTTGAACACAAAACCTACAACCTATTCACTTGCAACTGCCATTCATTTGTGGCCAACTGTCTCAATCGGCTCTGCTATGGCGGATCAATTAGTTGGAACATGATCAATGTGGCAGGCTTAGTACTGCTCAAGGGGCATTGGGTTGACGCCACATCCGTCTTGAGGTCGTTCCTCCCTTTTGTATTGGTGCTCGCTATTGGTGTTGCCATGGTTGGATGGCCATTCGTTGTTGTGCTTTTCTCCTTCTCGCTCCTCCTTCTGGTGTGGTTTATACTGGGCAGTTATTGTGTCAAGACCTTGTTAGAGTGCTAG

Protein Analysis

237

Amino Acids

26.66

Weight (kDa)

6.04

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 30 - 178 3.1e-73 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 133
AccII CGCG 1 cut(s) 394
AciI CCGC 4 cut(s) 131, 382, 394, 480
AclWI GGATC 2 cut(s) 83, 490
AcoI YGGCCR 4 cut(s) 71, 313, 450, 617
AcyI GRCGYC 1 cut(s) 546
AfaI GTAC 2 cut(s) 398, 522
AfiI CCNNNNNNNGG 5 cut(s) 164, 307, 308, 561, 658
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 1 cut(s) 404
AluBI AGCT 4 cut(s) 10, 20, 67, 197
AluI AGCT 4 cut(s) 10, 20, 67, 197
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 148, 464
AlwI GGATC 2 cut(s) 83, 490
AoxI GGCC 4 cut(s) 71, 313, 450, 617
AspS9I GGNCC 1 cut(s) 251
AvaII GGWCC 1 cut(s) 251
BalI TGGCCA 4 cut(s) 73, 315, 452, 619
BanI GGYRCC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 591
BccI CCATC 2 cut(s) 185, 609
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 148, 464
BfaI CTAG 1 cut(s) 709
BmcAI AGTACT 1 cut(s) 522
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 3 cut(s) 156, 217, 253
BmrI ACTGGG 1 cut(s) 683
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 683
BpuEI CTTGAG 2 cut(s) 512, 580
BsaHI GRCGYC 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 606
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 307, 308, 561, 658
Bse1I ACTGG 1 cut(s) 678
BseDI CCNNGG 1 cut(s) 606
BseGI GGATG 4 cut(s) 196, 376, 551, 620
BseLI CCNNNNNNNGG 5 cut(s) 164, 307, 308, 561, 658
BseNI ACTGG 1 cut(s) 678
BseRI GAGGAG 1 cut(s) 641
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 4 cut(s) 73, 315, 452, 619
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 591
BslFI GGGAC 2 cut(s) 237, 367
BslI CCNNNNNNNGG 5 cut(s) 164, 307, 308, 561, 658
BsmAI GTCTC 2 cut(s) 148, 464
BsmFI GGGAC 2 cut(s) 237, 367
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 4 cut(s) 73, 315, 452, 619
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 3 cut(s) 88, 482, 501
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 4 cut(s) 131, 382, 394, 480
BspANI GGCC 4 cut(s) 73, 315, 452, 619
BspFNI CGCG 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 156, 217, 253
BspPI GGATC 2 cut(s) 83, 490
BspT107I GGYRCC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 3 cut(s) 88, 482, 501
BssNI GRCGYC 1 cut(s) 546
BssT1I CCWWGG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 288, 458
BstACI GRCGYC 1 cut(s) 546
BstC8I GCNNGC 3 cut(s) 326, 514, 591
BstDEI CTNAG 1 cut(s) 517
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 4 cut(s) 196, 376, 551, 620
BstFNI CGCG 1 cut(s) 394
BstKTI GATC 3 cut(s) 91, 485, 504
BstMAI GTCTC 2 cut(s) 148, 464
BstMBI GATC 3 cut(s) 88, 482, 501
BstMWI GCNNNNNNNGC 2 cut(s) 321, 532
BstNSI RCATGY 1 cut(s) 173
BstUI CGCG 1 cut(s) 394
BsuRI GGCC 4 cut(s) 73, 315, 452, 619
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 4 cut(s) 196, 376, 551, 620
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 3 cut(s) 326, 514, 591
Cfr13I GGNCC 1 cut(s) 251
CseI GACGC 1 cut(s) 554
Csp6I GTAC 2 cut(s) 397, 521
CviAII CATG 5 cut(s) 62, 170, 329, 499, 607
CviQI GTAC 2 cut(s) 397, 521
DdeI CTNAG 1 cut(s) 517
DpnI GATC 3 cut(s) 90, 484, 503
DpnII GATC 3 cut(s) 88, 482, 501
EaeI YGGCCR 4 cut(s) 71, 313, 450, 617
EciI GGCGGA 1 cut(s) 495
Eco130I CCWWGG 1 cut(s) 606
Eco32I GATATC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 251
EcoRV GATATC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 5 cut(s) 65, 173, 332, 502, 610
FaqI GGGAC 2 cut(s) 237, 367
FatI CATG 5 cut(s) 61, 169, 328, 498, 606
FbaI TGATCA 1 cut(s) 501
FokI GGATG 4 cut(s) 203, 383, 538, 627
FspBI CTAG 1 cut(s) 709
HaeIII GGCC 4 cut(s) 73, 315, 452, 619
HgaI GACGC 1 cut(s) 554
Hin1I GRCGYC 1 cut(s) 546
Hin1II CATG 5 cut(s) 65, 173, 332, 502, 610
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 3 cut(s) 93, 167, 346
Hpy188III TCNNGA 2 cut(s) 559, 691
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 3 cut(s) 91, 652, 665
HpyCH4III ACNGT 2 cut(s) 288, 458
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 3 cut(s) 183, 324, 432
HpyF10VI GCNNNNNNNGC 2 cut(s) 321, 532
HpyF3I CTNAG 1 cut(s) 517
HpySE526I ACGT 1 cut(s) 365
Hsp92I GRCGYC 1 cut(s) 546
Hsp92II CATG 5 cut(s) 65, 173, 332, 502, 610
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 3 cut(s) 88, 482, 501
LmnI GCTCC 4 cut(s) 7, 138, 194, 654
LpnPI CCDG 7 cut(s) 169, 198, 274, 293, 498, 644, 659
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 709
MaeII ACGT 1 cut(s) 365
MalI GATC 3 cut(s) 90, 484, 503
MbiI CCGCTC 1 cut(s) 133
MboI GATC 3 cut(s) 88, 482, 501
MboII GAAGA 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 591
MlsI TGGCCA 4 cut(s) 73, 315, 452, 619
MluCI AATT 4 cut(s) 84, 220, 273, 486
MluNI TGGCCA 4 cut(s) 73, 315, 452, 619
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 2 cut(s) 473, 592
MnlI CCTC 5 cut(s) 52, 181, 555, 581, 662
Mox20I TGGCCA 4 cut(s) 73, 315, 452, 619
MscI TGGCCA 4 cut(s) 73, 315, 452, 619
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 4 cut(s) 73, 315, 452, 619
Mva1269I GAATGC 1 cut(s) 340
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 2 cut(s) 321, 532
NcoI CCATGG 1 cut(s) 606
NdeII GATC 3 cut(s) 88, 482, 501
NlaIII CATG 5 cut(s) 65, 173, 332, 502, 610
NlaIV GGNNCC 3 cut(s) 156, 217, 253
NspI RCATGY 1 cut(s) 173
PciI ACATGT 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PctI GAATGC 1 cut(s) 340
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PscI ACATGT 1 cut(s) 169
PspN4I GGNNCC 3 cut(s) 156, 217, 253
PspPI GGNCC 1 cut(s) 251
RsaI GTAC 2 cut(s) 398, 522
RsaNI GTAC 2 cut(s) 397, 521
RseI CAYNNNNRTG 1 cut(s) 333
Sau3AI GATC 3 cut(s) 88, 482, 501
Sau96I GGNCC 1 cut(s) 251
ScaI AGTACT 1 cut(s) 522
SchI GAGTC 1 cut(s) 121
SduI GDGCHC 1 cut(s) 591
SetI ASST 9 cut(s) 12, 22, 69, 199, 368, 416, 423, 566, 698
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 2 cut(s) 527, 559
SmoI CTYRAG 2 cut(s) 527, 559
Sse9I AATT 4 cut(s) 84, 220, 273, 486
SsiI CCGC 4 cut(s) 131, 382, 394, 480
SspI AATATT 1 cut(s) 55
SspMI CTAG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 2 cut(s) 288, 458
TaiI ACGT 1 cut(s) 368
TaqI TCGA 2 cut(s) 30, 78
TasI AATT 4 cut(s) 84, 220, 273, 486
TatI WGTACW 1 cut(s) 520
TscAI CASTG 1 cut(s) 293
TspDTI ATGAA 3 cut(s) 96, 151, 432
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 251
XceI RCATGY 1 cut(s) 173
XspI CTAG 1 cut(s) 709
ZrmI AGTACT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.