Rw6G028250

Protein REVERSION-TO-ETHYLENE SENSITIVITY1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004008
Physical Location & Seq
Forward (+)
52244295 .. 52245298
1004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028250.1

Sequence Viewer

Length: 675 bp
ATGGAGATAAAGAAAGCTTATGATATCGAACATATGTGCTCGACGCATGGCTCCAATTTTGTGAATGTTGATGATTTTGCATTTGGTGCTGTTGCTAGATATCTTCAACTTGATAGAAGACAGTGTTGTTTTTCCCCAAATTTGGGTAGCCACACTTGCAAGCATGGCTACAAGCATGCAAAGTTTGGGACTACAATCACCTGGGATGATGCCCCGCAATCGAGCACGCTCTACATTGAGCACAAAACCTACAACCTTTTCACTTGCAACAGTCACTCGTTCGTAGCAAACTTTCTGAATCGGATATGCTACAGTGGATCAATGCATTGGAACATGATAAATGTGGCAGCTTTAGTACTGTTCAAAGGGCATTGGGTTGATGCCATGTCTGTCTTGAAGTCATTCCTCCCTTTTCTTCTGGTGTATACATGGTTGGGTGGCCATTCGTGGTGGCGCTTTTCTCCTTCTCCTCTCCTCTTGCTGTGGTTTGTACTTGGCACTTATTGTTTTAAGACTGTTAGAGTGCTAGTTCTTCCTTCTTCTTCTTTTTTTTTTTTTTTTAAGGGACAATTTTTGAACAGTTTATGGAATGTTCTTGACCAAAAGCACCAAAATGAACAAAAATTGTCCCACTTACCCCAGCAACAGATTTTTATTCCCACATACACAATTTAA

Protein Analysis

224

Amino Acids

26.06

Weight (kDa)

8.95

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RTE1 PF05608 16 - 123 1.4e-37 RTE1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 425
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 2 cut(s) 357, 492
AfiI CCNNNNNNNGG 2 cut(s) 142, 143
AgsI TTSAA 4 cut(s) 107, 364, 397, 577
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 2 cut(s) 17, 350
AluI AGCT 2 cut(s) 17, 350
Alw21I GWGCWC 3 cut(s) 41, 227, 243
AlwI GGATC 1 cut(s) 325
AoxI GGCC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 347
ApoI RAATTY 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 401
AspLEI GCGC 1 cut(s) 456
AsuHPI GGTGA 1 cut(s) 190
BalI TGGCCA 1 cut(s) 441
BbsI GAAGAC 1 cut(s) 124
Bbv12I GWGCWC 3 cut(s) 41, 227, 243
BbvI GCAGC 1 cut(s) 359
BciT130I CCWGG 1 cut(s) 202
BfaI CTAG 2 cut(s) 96, 527
BfmI CTRYAG 1 cut(s) 310
BfoI RGCGCY 1 cut(s) 457
BisI GCNGC 1 cut(s) 348
BlsI GCNGC 1 cut(s) 349
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 2 cut(s) 199, 370
BpiI GAAGAC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 143
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 2 cut(s) 142, 143
BseRI GAGGAG 2 cut(s) 459, 464
BseXI GCAGC 1 cut(s) 359
BseYI CCCAGC 1 cut(s) 639
BshFI GGCC 1 cut(s) 441
BsiHKAI GWGCWC 3 cut(s) 41, 227, 243
BslFI GGGAC 3 cut(s) 202, 579, 613
BslI CCNNNNNNNGG 2 cut(s) 142, 143
BsmFI GGGAC 3 cut(s) 202, 579, 613
BsnI GGCC 1 cut(s) 441
Bsp1286I GDGCHC 3 cut(s) 41, 227, 243
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 1 cut(s) 215
BspANI GGCC 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 1 cut(s) 317
BssNAI GTATAC 1 cut(s) 426
Bst1107I GTATAC 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 6 cut(s) 123, 272, 314, 360, 517, 581
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 3 cut(s) 161, 177, 227
BstF5I GGATG 1 cut(s) 211
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 1 cut(s) 320
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 3 cut(s) 86, 156, 165
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 359
BstV2I GAAGAC 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 426
BsuRI GGCC 1 cut(s) 441
BtsCI GGATG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 128, 319
Cac8I GCNNGC 3 cut(s) 161, 177, 227
CfoI GCGC 1 cut(s) 456
CseI GACGC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 356, 491
CviAII CATG 6 cut(s) 47, 164, 176, 334, 385, 429
CviJI RGCY 6 cut(s) 17, 51, 150, 168, 350, 441
CviKI_1 RGCY 6 cut(s) 17, 51, 150, 168, 350, 441
CviQI GTAC 2 cut(s) 356, 491
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 439
Eco32I GATATC 2 cut(s) 25, 101
EcoRII CCWGG 1 cut(s) 200
EcoRV GATATC 2 cut(s) 25, 101
EcoT22I ATGCAT 1 cut(s) 327
FaeI CATG 6 cut(s) 50, 167, 179, 337, 388, 432
FaqI GGGAC 3 cut(s) 202, 579, 613
FatI CATG 6 cut(s) 46, 163, 175, 333, 384, 428
FauI CCCGC 1 cut(s) 222
FauNDI CATATG 1 cut(s) 33
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 1 cut(s) 348
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 1 cut(s) 348
FspBI CTAG 2 cut(s) 96, 527
GlaI GCGC 1 cut(s) 455
GluI GCNGC 1 cut(s) 348
GsaI CCCAGC 1 cut(s) 643
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 1 cut(s) 441
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 456
Hin1II CATG 6 cut(s) 50, 167, 179, 337, 388, 432
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 1 cut(s) 298
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 2 cut(s) 297, 303
Hpy188III TCNNGA 2 cut(s) 394, 596
Hpy8I GTNNAC 1 cut(s) 426
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 474, 546
HpyCH4III ACNGT 6 cut(s) 123, 272, 314, 360, 517, 581
HpyCH4V TGCA 5 cut(s) 80, 159, 179, 267, 325
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 156, 165
Hsp92II CATG 6 cut(s) 50, 167, 179, 337, 388, 432
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 4 cut(s) 187, 214, 404, 653
Lsp1109I GCAGC 1 cut(s) 359
LweI GCATC 2 cut(s) 199, 370
MaeI CTAG 2 cut(s) 96, 527
MaeIII GTNAC 1 cut(s) 272
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 6 cut(s) 95, 129, 407, 524, 531, 534
MhlI GDGCHC 3 cut(s) 41, 227, 243
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 5 cut(s) 55, 139, 569, 623, 669
MluNI TGGCCA 1 cut(s) 441
MnlI CCTC 3 cut(s) 416, 480, 485
Mox20I TGGCCA 1 cut(s) 441
Mph1103I ATGCAT 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 401
MscI TGGCCA 1 cut(s) 441
MseI TTAA 3 cut(s) 510, 561, 673
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 202
MvaI CCWGG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 86, 156, 165
NdeI CATATG 1 cut(s) 33
NdeII GATC 1 cut(s) 317
NlaIII CATG 6 cut(s) 50, 167, 179, 337, 388, 432
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 272
NsiI ATGCAT 1 cut(s) 327
NspI RCATGY 1 cut(s) 179
PaeI GCATGC 1 cut(s) 179
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 200
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 52
RsaI GTAC 2 cut(s) 357, 492
RsaNI GTAC 2 cut(s) 356, 491
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 3 cut(s) 510, 561, 673
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 1 cut(s) 317
ScaI AGTACT 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 3 cut(s) 41, 227, 243
SetI ASST 5 cut(s) 19, 203, 251, 258, 352
SfaNI GCATC 2 cut(s) 199, 370
SfcI CTRYAG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 612
SphI GCATGC 1 cut(s) 179
Sse9I AATT 5 cut(s) 55, 139, 569, 623, 669
SsiI CCGC 1 cut(s) 215
SspMI CTAG 2 cut(s) 96, 527
StyD4I CCNGG 1 cut(s) 200
TaaI ACNGT 6 cut(s) 123, 272, 314, 360, 517, 581
TaqI TCGA 3 cut(s) 27, 41, 221
TasI AATT 5 cut(s) 55, 139, 569, 623, 669
TatI WGTACW 2 cut(s) 355, 490
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 3 cut(s) 510, 561, 673
Tru9I TTAA 3 cut(s) 510, 561, 673
TscAI CASTG 2 cut(s) 128, 319
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 1 cut(s) 347
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 630
TspRI CASTG 2 cut(s) 128, 319
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 401
XspI CTAG 2 cut(s) 96, 527
ZrmI AGTACT 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.