MD08G1066200.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010799
Physical Location & Seq
Forward (+)
5300967 .. 5305494
4528 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1066200.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGGAGGATCTGGAGGAGCAGGAGGAGGAGGAGGTGCTGGGTTCGAGCTTGACGATGGAGAAGGTGGCGGCGGCCAAGCAGTTCATAGAGAACCACTACAGAGCTCAGATGAAGAACATTCAGGAGCGGAAAGAGAGACGATGGGTGTTGGAAAGGAAATTAGCTTCGTCAGATGTGCCAAAGGAGGTACAAGTCAACCTGATTAAAGACTTGGAGCGAAAAGAGACAGAATTTATGCGACTTAAAAGGCACAAGATATGTGTTGATGATTTTGAGCCTTTGACCATCATTGGTAGGGGGGCCTTTGGTGAGGTCCGATTGTGTCGGGAGAAAAAATCTGGCAATATCTACGCCATGAAAAAGTTGAAGAAATCTGAAATGCTGAAGAGAGGACAGGTGGAACATGTTAGAGCTGAAAGAAACTTGCTGGCAGAAGTTGCCAGCCATTGCATAGTTAAACTTTACTACTCCTTCCAGGACGATGAGTACTTATATTTAATAATGGAATATCTGCCTGGGGGTGACATGATGACTCTGCTGATTAGGGAAGACACCTTGACTGAAAGTGTGGCTAAATTTTACATTGCACAGAGCGTACTGGCCATAGAATCTATTCAGAAACACAACTACATACACAGAGACATTAAGCCAGACAACCTGCTTCTGGATAAAAATGGTCACATGAAACTCTCGGATTTTGGCCTCTGTAAGCCTCTTGATTGTACAACTTTACCTTCACTGCATGAAAATAGAACTATGGATGATGAAAATATGACAGAACCAATGGATATCGACACATGCGTTCCTGATGCGGATAATCGGAGTAGTTGGAAAAGCCCCGCCGAACAGCTGCAACATTGGCAGATGAACAGGAGGAAGTTGGCATTTTCAACTGTGGGGACACCCGACTATATTGCACCTGAAGTGTTATTGAAGAAAGGATATGGAATGGAATGCGACTGGTGGTCATTGGGAGCAATAATGTATGAAATGCTTGTTGGTTACCCTCCATTTTATTCAGATGATCCAATAACTACATGCAGAAAGATTGTTCATTGGAAAAACCACTTAAAATTTCCAGAAGATGCAAGGTTGACAGCTGAGGCTAAGGATCTTATTTGCAGGTTGCTGTGTGATGTTGAACATAGGCTAGGGACTGGAGGGGCACATCAAATCAAATCTCATCCTTGGTTCAAGGATGTTGTGTGGGACAAACTCTATGAAATGGACGCAGCATTCAAACCAGAAGTGAATGGAGAGCTTGATACCCAAAACTTTATGAAGTTTGATGAGTTGGACCCACCAGCGTCAGCAAGAACTGGATCTTCACGAAAGTTGCAAATGACTCCCAAAGATTTAAGCTTTGTTGGCTATACATACAAGAACTTTGATGCTGTGAAAGGGCTTCGTCAAGCTCTTGGTGATGCTAGGGTAGATTTTACTGCTGAACGGGCAGCCAAGGAAGCCGAGTTACAGATGCTTGCATCAGCCGGTGATCCCATGTTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

504

Amino Acids

58.07

Weight (kDa)

5.73

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 92 - 240 6.9e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 96 - 239 1.3e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 165 - 241 2.1e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 289 - 398 2.9e-21 Protein kinase domain
Pkinase_C PF00433 417 - 460 4.6e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33080 AT4G33080
fragaria_vesca FvH4_2g31460 FvH4_2g31460
malus_domestica MD08G1066200.v1.1 MD15G1055000.v1.1
prunus_persica Prupe.1G408200_v2.0.a1
pyrus_communis pycom08g05490 pycom15g05190
rosa_chinensis RchiOBHm_Chr6g0311341
rosa_laevigata RLG00000010415
rosa_multiflora Rmu_sc0008536.1_g000007
rosa_roxburghii Rroxscaffold_7G00157700
rosa_rugosa Rorug06G0387700 Rorug06G0387700
rosa_samantha Rh6AG502400 Rh6BG513000 Rh6CG518800 Rh6DG505000
rosa_wichuraiana Rw6G043750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 666, 1113
AccBSI CCGCTC 1 cut(s) 127
AciI CCGC 5 cut(s) 68, 71, 127, 812, 840
AclWI GGATC 5 cut(s) 15, 1019, 1119, 1330, 1490
AcoI YGGCCR 2 cut(s) 72, 600
AcsI RAATTY 3 cut(s) 230, 575, 1073
AcuI CTGAAG 2 cut(s) 404, 942
AfaI GTAC 4 cut(s) 189, 488, 597, 724
AfiI CCNNNNNNNGG 1 cut(s) 664
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 6 cut(s) 367, 891, 934, 1142, 1195, 1240
AhdI GACNNNNNGTC 1 cut(s) 964
AjnI CCWGG 2 cut(s) 474, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 981, 1013
AluBI AGCT 9 cut(s) 48, 104, 164, 413, 850, 1100, 1261, 1362, 1415
AluI AGCT 9 cut(s) 48, 104, 164, 413, 850, 1100, 1261, 1362, 1415
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 3 cut(s) 130, 218, 633
AlwI GGATC 5 cut(s) 15, 1019, 1119, 1330, 1490
AoxI GGCC 4 cut(s) 72, 300, 600, 700
ApeKI GCWGC 3 cut(s) 850, 1232, 1454
ApoI RAATTY 3 cut(s) 230, 575, 1073
Asp700I GAANNNNTTC 1 cut(s) 612
AspS9I GGNCC 3 cut(s) 300, 313, 1297
AsuHPI GGTGA 4 cut(s) 320, 533, 1433, 1505
AvaII GGWCC 2 cut(s) 313, 1297
BaeGI GKGCMC 1 cut(s) 1168
BalI TGGCCA 1 cut(s) 602
BanII GRGCYC 1 cut(s) 106
BarI GAAGNNNNNNTAC 2 cut(s) 1455, 1487
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 3 cut(s) 837, 1244, 1466
BccI CCATC 3 cut(s) 49, 135, 293
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 2 cut(s) 476, 516
BcoDI GTCTC 3 cut(s) 130, 218, 633
BfaI CTAG 2 cut(s) 1151, 1428
BfmI CTRYAG 1 cut(s) 97
BfuAI ACCTGC 2 cut(s) 666, 1113
BisI GCNGC 5 cut(s) 69, 72, 851, 1233, 1455
BlsI GCNGC 5 cut(s) 70, 73, 852, 1234, 1456
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 476, 516
Bme18I GGWCC 2 cut(s) 313, 1297
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgT120I GGNCC 3 cut(s) 300, 313, 1297
BmiI GGNNCC 2 cut(s) 301, 1299
BmrFI CCNGG 2 cut(s) 476, 516
BmsI GCATC 6 cut(s) 799, 1075, 1381, 1414, 1467, 1493
BpiI GAAGAC 1 cut(s) 555
BpmI CTGGAG 2 cut(s) 32, 1179
Bpu10I CCTNAGC 2 cut(s) 1101, 1107
BsaJI CCNNGG 3 cut(s) 515, 1187, 1458
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse118I RCCGGY 1 cut(s) 1490
Bse1I ACTGG 4 cut(s) 603, 965, 1162, 1324
Bse3DI GCAATG 2 cut(s) 445, 582
BseBI CCWGG 2 cut(s) 476, 516
BseDI CCNNGG 3 cut(s) 515, 1187, 1458
BseGI GGATG 3 cut(s) 766, 1183, 1204
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 2 cut(s) 445, 582
BseMII CTCAG 2 cut(s) 119, 1092
BseNI ACTGG 4 cut(s) 603, 965, 1162, 1324
BseRI GAGGAG 4 cut(s) 29, 38, 41, 44
BseSI GKGCMC 1 cut(s) 1168
BseXI GCAGC 3 cut(s) 837, 1244, 1466
BseYI CCCAGC 1 cut(s) 37
BshFI GGCC 4 cut(s) 74, 302, 602, 702
BsiHKAI GWGCWC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 1491
BslFI GGGAC 3 cut(s) 913, 1168, 1223
BslI CCNNNNNNNGG 1 cut(s) 664
BsmAI GTCTC 3 cut(s) 130, 218, 633
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 3 cut(s) 913, 1168, 1223
BsmI GAATGC 2 cut(s) 959, 1235
BsnI GGCC 4 cut(s) 74, 302, 602, 702
Bsp1286I GDGCHC 2 cut(s) 106, 1168
Bsp1407I TGTACA 1 cut(s) 722
Bsp143I GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
BspACI CCGC 5 cut(s) 68, 71, 127, 812, 840
BspANI GGCC 4 cut(s) 74, 302, 602, 702
BspCNI CTCAG 2 cut(s) 118, 1093
BspLI GGNNCC 2 cut(s) 301, 1299
BspMI ACCTGC 2 cut(s) 666, 1113
BspPI GGATC 5 cut(s) 15, 1019, 1119, 1330, 1490
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 445, 582
BsrFI RCCGGY 1 cut(s) 1490
BsrGI TGTACA 1 cut(s) 722
BsrI ACTGG 4 cut(s) 603, 965, 1162, 1324
BssAI RCCGGY 1 cut(s) 1490
BssECI CCNNGG 3 cut(s) 515, 1187, 1458
BssMI GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
BssT1I CCWWGG 2 cut(s) 1187, 1458
Bst2UI CCWGG 2 cut(s) 476, 516
Bst4CI ACNGT 1 cut(s) 895
Bst6I CTCTTC 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 429, 442, 1482
BstDEI CTNAG 3 cut(s) 105, 1101, 1107
BstEII GGTNACC 1 cut(s) 1001
BstF5I GGATG 3 cut(s) 766, 1183, 1204
BstKTI GATC 5 cut(s) 10, 1027, 1114, 1325, 1498
BstMAI GTCTC 3 cut(s) 130, 218, 633
BstMBI GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
BstMWI GCNNNNNNNGC 5 cut(s) 437, 859, 1368, 1451, 1463
BstNI CCWGG 2 cut(s) 476, 516
BstNSI RCATGY 3 cut(s) 407, 801, 1041
BstPI GGTNACC 1 cut(s) 1001
BstSCI CCNGG 2 cut(s) 474, 514
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 1168
BstV1I GCAGC 3 cut(s) 837, 1244, 1466
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 3 cut(s) 7, 1111, 1322
BstYI RGATCY 3 cut(s) 7, 1111, 1322
BsuRI GGCC 4 cut(s) 74, 302, 602, 702
BtsCI GGATG 3 cut(s) 766, 1183, 1204
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 1 cut(s) 737
BveI ACCTGC 2 cut(s) 666, 1113
Cac8I GCNNGC 3 cut(s) 429, 442, 1482
Cfr10I RCCGGY 1 cut(s) 1490
Cfr13I GGNCC 3 cut(s) 300, 313, 1297
CseI GACGC 2 cut(s) 1238, 1296
Csp6I GTAC 4 cut(s) 188, 487, 596, 723
CviAII CATG 8 cut(s) 355, 404, 526, 682, 743, 798, 1038, 1501
CviQI GTAC 4 cut(s) 188, 487, 596, 723
DdeI CTNAG 3 cut(s) 105, 1101, 1107
DpnI GATC 5 cut(s) 9, 1026, 1113, 1324, 1497
DpnII GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
DriI GACNNNNNGTC 1 cut(s) 964
EaeI YGGCCR 2 cut(s) 72, 600
Eam1104I CTCTTC 1 cut(s) 380
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 104
Eco130I CCWWGG 2 cut(s) 1187, 1458
Eco24I GRGCYC 1 cut(s) 106
Eco32I GATATC 1 cut(s) 790
Eco47I GGWCC 2 cut(s) 313, 1297
Eco53kI GAGCTC 1 cut(s) 104
Eco57I CTGAAG 2 cut(s) 404, 942
Eco91I GGTNACC 1 cut(s) 1001
EcoICRI GAGCTC 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 300
EcoO65I GGTNACC 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 474, 514
EcoRV GATATC 1 cut(s) 790
EcoT14I CCWWGG 2 cut(s) 1187, 1458
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 2 cut(s) 1187, 1458
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 8 cut(s) 358, 407, 529, 685, 746, 801, 1041, 1504
FaqI GGGAC 3 cut(s) 913, 1168, 1223
FatI CATG 8 cut(s) 354, 403, 525, 681, 742, 797, 1037, 1500
FauI CCCGC 1 cut(s) 847
Fnu4HI GCNGC 5 cut(s) 69, 72, 851, 1233, 1455
FokI GGATG 3 cut(s) 773, 1170, 1211
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 5 cut(s) 69, 72, 851, 1233, 1455
FspBI CTAG 2 cut(s) 1151, 1428
GluI GCNGC 5 cut(s) 69, 72, 851, 1233, 1455
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 2 cut(s) 32, 1179
HaeIII GGCC 4 cut(s) 74, 302, 602, 702
HapII CCGG 1 cut(s) 1491
HgaI GACGC 2 cut(s) 1238, 1296
Hin1II CATG 8 cut(s) 358, 407, 529, 685, 746, 801, 1041, 1504
HincII GTYRAC 2 cut(s) 196, 1095
HindII GTYRAC 2 cut(s) 196, 1095
HindIII AAGCTT 1 cut(s) 1360
HinfI GANTC 3 cut(s) 532, 608, 1345
HpaII CCGG 1 cut(s) 1491
HphI GGTGA 4 cut(s) 320, 533, 1433, 1505
Hpy166II GTNNAC 2 cut(s) 196, 1095
Hpy188I TCNGA 8 cut(s) 108, 172, 317, 376, 618, 694, 822, 1021
Hpy188III TCNNGA 8 cut(s) 11, 122, 326, 665, 716, 806, 1079, 1329
Hpy8I GTNNAC 2 cut(s) 196, 1095
HpyAV CCTTC 3 cut(s) 55, 481, 744
HpyCH4III ACNGT 1 cut(s) 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 437, 859, 1368, 1451, 1463
HpyF3I CTNAG 3 cut(s) 105, 1101, 1107
Hsp92II CATG 8 cut(s) 358, 407, 529, 685, 746, 801, 1041, 1504
Kzo9I GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
LmnI GCTCC 4 cut(s) 16, 124, 214, 974
Lsp1109I GCAGC 3 cut(s) 837, 1244, 1466
LweI GCATC 6 cut(s) 799, 1075, 1381, 1414, 1467, 1493
MaeI CTAG 2 cut(s) 1151, 1428
MaeIII GTNAC 5 cut(s) 521, 677, 1001, 1470, 1503
MalI GATC 5 cut(s) 9, 1026, 1113, 1324, 1497
MbiI CCGCTC 1 cut(s) 127
MboI GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
MboII GAAGA 7 cut(s) 124, 379, 397, 560, 946, 1094, 1317
MflI RGATCY 3 cut(s) 7, 1111, 1322
MhlI GDGCHC 2 cut(s) 106, 1168
MlsI TGGCCA 1 cut(s) 602
MluCI AATT 4 cut(s) 158, 230, 575, 1073
MluNI TGGCCA 1 cut(s) 602
MlyI GAGTC 2 cut(s) 526, 1339
MmeI TCCRAC 3 cut(s) 129, 809, 1275
Mox20I TGGCCA 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 1 cut(s) 602
MseI TTAA 7 cut(s) 204, 243, 456, 497, 645, 1070, 1358
Msp20I TGGCCA 1 cut(s) 602
MspA1I CMGCKG 2 cut(s) 850, 1100
MspI CCGG 1 cut(s) 1491
MspR9I CCNGG 2 cut(s) 476, 516
Mva1269I GAATGC 2 cut(s) 959, 1235
MvaI CCWGG 2 cut(s) 476, 516
MwoI GCNNNNNNNGC 5 cut(s) 437, 859, 1368, 1451, 1463
NdeII GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
NlaIII CATG 8 cut(s) 358, 407, 529, 685, 746, 801, 1041, 1504
NlaIV GGNNCC 2 cut(s) 301, 1299
NmeAIII GCCGAG 1 cut(s) 1492
NmuCI GTSAC 2 cut(s) 521, 677
NspI RCATGY 3 cut(s) 407, 801, 1041
PciI ACATGT 1 cut(s) 403
PcsI WCGNNNNNNNCGW 2 cut(s) 50, 798
PctI GAATGC 2 cut(s) 959, 1235
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 1 cut(s) 608
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 5 cut(s) 70, 73, 852, 1234, 1456
PleI GAGTC 2 cut(s) 526, 1339
PpsI GAGTC 2 cut(s) 526, 1339
PscI ACATGT 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 474, 514
PspEI GGTNACC 1 cut(s) 1001
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 474, 514
PspN4I GGNNCC 2 cut(s) 301, 1299
PspPI GGNCC 3 cut(s) 300, 313, 1297
PsuI RGATCY 3 cut(s) 7, 1111, 1322
PvuII CAGCTG 2 cut(s) 850, 1100
RsaI GTAC 4 cut(s) 189, 488, 597, 724
RsaNI GTAC 4 cut(s) 188, 487, 596, 723
SacI GAGCTC 1 cut(s) 106
SaqAI TTAA 7 cut(s) 204, 243, 456, 497, 645, 1070, 1358
SatI GCNGC 5 cut(s) 69, 72, 851, 1233, 1455
Sau3AI GATC 5 cut(s) 7, 1024, 1111, 1322, 1495
Sau96I GGNCC 3 cut(s) 300, 313, 1297
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 2 cut(s) 526, 1339
ScrFI CCNGG 2 cut(s) 476, 516
SduI GDGCHC 2 cut(s) 106, 1168
SfaNI GCATC 6 cut(s) 799, 1075, 1381, 1414, 1467, 1493
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 2 cut(s) 313, 1297
Sse9I AATT 4 cut(s) 158, 230, 575, 1073
SsiI CCGC 5 cut(s) 68, 71, 127, 812, 840
SspMI CTAG 2 cut(s) 1151, 1428
SstI GAGCTC 1 cut(s) 106
StyD4I CCNGG 2 cut(s) 474, 514
StyI CCWWGG 2 cut(s) 1187, 1458
TaaI ACNGT 1 cut(s) 895
TaqI TCGA 2 cut(s) 44, 792
TasI AATT 4 cut(s) 158, 230, 575, 1073
TatI WGTACW 2 cut(s) 486, 722
TauI GCSGC 2 cut(s) 71, 74
TfiI GAWTC 1 cut(s) 608
Tru1I TTAA 7 cut(s) 204, 243, 456, 497, 645, 1070, 1358
Tru9I TTAA 7 cut(s) 204, 243, 456, 497, 645, 1070, 1358
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 2 cut(s) 521, 677
TseI GCWGC 3 cut(s) 850, 1232, 1454
Tsp45I GTSAC 2 cut(s) 521, 677
TspRI CASTG 1 cut(s) 744
VpaK11BI GGWCC 2 cut(s) 313, 1297
XapI RAATTY 3 cut(s) 230, 575, 1073
XceI RCATGY 3 cut(s) 407, 801, 1041
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 1151, 1428
ZrmI AGTACT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.