Rroxscaffold_7G00157700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010799
Physical Location & Seq
Reverse (-)
1427387 .. 1435120
7734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157700.1

Sequence Viewer

Length: 1725 bp
ATGGAGGATCGACTGGAGGAGGAAGGAGAAGGAGAAGGAGAGGAGGTGTTAGGTTCGAGCTTGACGATGGAGAAGGTCGCAGCAGCCAAGCAGTTCATAGAGAACCACTACAGAGCTCAGAGGAAGAACATACAAGAACGAAAAGAAAGACGATGGGTTTTGGAAAGGAAATTAGCTTCATCAGACGTGCCAAAGGAGGTACAAATCAACCTGATCAAAGATTTAGAGCGAAAAGAGACGGAGTTCATGCGACTTAAAAGGCATAGGATATGTGTTGACGATTTTGAGCCTTTGACCATCATTGGTTCGATTGTGTCGGGAGAAAAATCTGGCAATATATATGCCATGAAAAAGTTGAAGAAATCTGAAATGCTTATGAGAGGACAGGTGGAACATGTTAGAGCTGAAAGAAACTTGCTGGCAGAAGTTGCCAGTCATTGCATAGTTAAACTTTACTACTCCTTTCAGGATGCTGAGTATTTATATTTAATAATGGAATATCTGCCTGGTGGTGACATGATGACTCTGCTGATGAGGGAGGACACCTTAAATGAAAGTGTGGCCAAATTTTACATTGCACAGAGTGTACTGGCAATAGAATCTATTCAGAAACACAACTACATACACAGAGACATTAAACCTGACAACCTTCTTCTAGATAAAAACGGTCACATGAAACTCTCGGATTTTGGCCTCTGTAAGCCTCTTGATTGTACAACTTTACCTGCACTGCATGAAAATAGATCTATGGATGATGAAAATTCAGCAGAACCAATGGATATCGATGGATGTTTTCCTGATGCGGATAACAGGAATAGTTGGAAAAGCCCTCTTGAACAGCTGCAGCATTGGCAGATGAACAGGAGGAAGCTGGCATTTTCGACTGTGGGGACACCTGACTATATTGCACCTGAAGTGTTATTAAAGAAGGGATATGGGATGGAATGTGACTGGTGGTCACTGGGAGCAATACTGTATGAAATGCTTGTTGGATACCCTCCATTTTATTCAGATGATCCCATAACTACATGCAGAAAGATTGTTCATTGGAGAAATCATTTAAAATTTCCAGAAGATGCAAGGCTGACACTTGAGGCTAAGGATCTCATTTGTAGGTTGCTGTGTGATGTTGAACATAGGCTTGGTGCACGTCAAATCAAATCTCATCCTTGGTTCAAGGACATTGTGTGGGACAAACTTTATGAAATGGAGGCAGCATTCAAACCAGAAGTGAATGGGGAGCTTGATACCCAAAACTTTATGAAGTTTGATGAGCTGGATCCTCCGGCATCAGCAAGAACTGGTTCAGGACCCTCGCGGAAGCTGCAATTGACTCCCAAAGATCTAAACTTTGTTGGCTATACATACAAGAACTTTGATGCTGTAAAAGGGCTTCGCCAAGCTTTTGCTGATGCTCGAGTAGAATTCACTACTGAACGGGCTGCAAGAGAAACAGAGGTACAAATGCTTGCATCGTCTGCTTGCATGCCGCCTTCATCTAAGTGTACAGTAGTCATTGAAGAGCTTCATAAAGACCCTGACAAGGTAGCGGTGATATTGATGGGGACGGTTGCTTTTCTCGCCGCTTATCTTGTTGGTGGTGACATCTCTTTGAGTGGTGGGTTCGTTGGTGAAGCCAACGTTCATGCTAAGGTCGACGACTTCGGGGGAGGCTCGTGGTGTTTGCCGATACCGCAGTTGTCTTCGATGGTGTGGCGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

574

Amino Acids

65.64

Weight (kDa)

5.6

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 108 - 237 3.5e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 108 - 236 3.9e-18 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 162 - 238 1.5e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 286 - 392 3.1e-22 Protein kinase domain
Pkinase_C PF00433 411 - 456 1.8e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33080 AT4G33080
fragaria_vesca FvH4_2g31460 FvH4_2g31460
malus_domestica MD08G1066200.v1.1 MD15G1055000.v1.1
prunus_persica Prupe.1G408200_v2.0.a1
pyrus_communis pycom08g05490 pycom15g05190
rosa_chinensis RchiOBHm_Chr6g0311341
rosa_laevigata RLG00000010415
rosa_multiflora Rmu_sc0008536.1_g000007
rosa_roxburghii Rroxscaffold_7G00157700
rosa_rugosa Rorug06G0387700 Rorug06G0387700
rosa_samantha Rh6AG502400 Rh6BG513000 Rh6CG518800 Rh6DG505000
rosa_wichuraiana Rw6G043750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 733
AccI GTMKAC 1 cut(s) 1656
AccII CGCG 1 cut(s) 1318
AciI CCGC 7 cut(s) 803, 1318, 1490, 1550, 1584, 1694, 1717
AclI AACGTT 1 cut(s) 1641
AclWI GGATC 5 cut(s) 15, 1010, 1110, 1274, 1287
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 4 cut(s) 566, 760, 1064, 1424
AcuI CTGAAG 1 cut(s) 933
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 5 cut(s) 201, 588, 715, 1461, 1507
AfiI CCNNNNNNNGG 1 cut(s) 1543
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 6 cut(s) 358, 836, 1133, 1177, 1222, 1520
AhdI GACNNNNNGTC 1 cut(s) 955
AjiI CACGTC 2 cut(s) 187, 1151
AjnI CCWGG 1 cut(s) 505
AjuI GAANNNNNNNTTGG 4 cut(s) 972, 1004, 1125, 1157
Alw21I GWGCWC 2 cut(s) 118, 1150
Alw26I GTCTC 2 cut(s) 230, 624
Alw44I GTGCAC 1 cut(s) 1146
AlwI GGATC 5 cut(s) 15, 1010, 1110, 1274, 1287
Ama87I CYCGRG 1 cut(s) 1416
AoxI GGCC 2 cut(s) 561, 691
ApaLI GTGCAC 1 cut(s) 1146
ApeKI GCWGC 7 cut(s) 80, 83, 841, 844, 1214, 1324, 1442
ApoI RAATTY 4 cut(s) 566, 760, 1064, 1424
Asp700I GAANNNNTTC 3 cut(s) 603, 1303, 1524
AspS9I GGNCC 1 cut(s) 1310
AsuHPI GGTGA 4 cut(s) 524, 1564, 1613, 1643
AvaI CYCGRG 1 cut(s) 1416
AvaII GGWCC 1 cut(s) 1310
BaeGI GKGCMC 1 cut(s) 1150
BaeI ACNNNNGTAYC 2 cut(s) 1682, 1715
BalI TGGCCA 1 cut(s) 563
BamHI GGATCC 1 cut(s) 1279
BanII GRGCYC 1 cut(s) 118
BarI GAAGNNNNNNTAC 2 cut(s) 1377, 1409
BauI CACGAG 1 cut(s) 1675
BbsI GAAGAC 1 cut(s) 1695
Bbv12I GWGCWC 2 cut(s) 118, 1150
BbvI GCAGC 7 cut(s) 92, 95, 828, 856, 1226, 1311, 1429
BccI CCATC 7 cut(s) 61, 147, 305, 779, 934, 1555, 1702
BciT130I CCWGG 1 cut(s) 507
BciVI GTATCC 1 cut(s) 986
BclI TGATCA 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 230, 624
BfaI CTAG 1 cut(s) 656
BfmI CTRYAG 2 cut(s) 109, 842
BfuAI ACCTGC 1 cut(s) 733
BfuI GTATCC 1 cut(s) 986
BglII AGATCT 2 cut(s) 743, 1342
BisI GCNGC 9 cut(s) 81, 84, 842, 845, 1215, 1325, 1443, 1490, 1584
BlsI GCNGC 9 cut(s) 82, 85, 843, 846, 1216, 1326, 1444, 1491, 1585
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 1 cut(s) 1310
BmeRI GACNNNNNGTC 1 cut(s) 955
BmeT110I CYCGRG 1 cut(s) 1416
BmgBI CACGTC 2 cut(s) 187, 1151
BmgT120I GGNCC 1 cut(s) 1310
BmiI GGNNCC 2 cut(s) 1281, 1312
BmrFI CCNGG 1 cut(s) 507
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 7 cut(s) 460, 790, 1066, 1298, 1369, 1402, 1481
BmuI ACTGGG 1 cut(s) 971
BpiI GAAGAC 1 cut(s) 1695
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 2 cut(s) 1098, 1650
BpuEI CTTGAG 1 cut(s) 1112
Bsa29I ATCGAT 1 cut(s) 783
BsaJI CCNNGG 1 cut(s) 1169
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 1543
Bse1I ACTGG 6 cut(s) 18, 432, 594, 956, 966, 1306
Bse3DI GCAATG 2 cut(s) 436, 573
BseBI CCWGG 1 cut(s) 507
BseCI ATCGAT 1 cut(s) 783
BseDI CCNNGG 1 cut(s) 1169
BseGI GGATG 5 cut(s) 475, 757, 794, 945, 1165
BseLI CCNNNNNNNGG 1 cut(s) 1543
BseMI GCAATG 2 cut(s) 436, 573
BseMII CTCAG 2 cut(s) 131, 465
BseNI ACTGG 6 cut(s) 18, 432, 594, 956, 966, 1306
BseRI GAGGAG 2 cut(s) 32, 56
BseSI GKGCMC 1 cut(s) 1150
BseXI GCAGC 7 cut(s) 92, 95, 828, 856, 1226, 1311, 1429
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 1 cut(s) 1318
BshFI GGCC 2 cut(s) 563, 693
BshVI ATCGAT 1 cut(s) 783
BsiHKAI GWGCWC 2 cut(s) 118, 1150
BsiHKCI CYCGRG 1 cut(s) 1416
BsiSI CCGG 1 cut(s) 1286
BslFI GGGAC 3 cut(s) 904, 1205, 1579
BslI CCNNNNNNNGG 1 cut(s) 1543
BsmAI GTCTC 2 cut(s) 230, 624
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 3 cut(s) 904, 1205, 1579
BsmI GAATGC 1 cut(s) 1217
BsnI GGCC 2 cut(s) 563, 693
BsoBI CYCGRG 1 cut(s) 1416
Bsp1286I GDGCHC 2 cut(s) 118, 1150
Bsp1407I TGTACA 2 cut(s) 713, 1505
Bsp143I GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
BspACI CCGC 7 cut(s) 803, 1318, 1490, 1550, 1584, 1694, 1717
BspANI GGCC 2 cut(s) 563, 693
BspCNI CTCAG 2 cut(s) 130, 466
BspDI ATCGAT 1 cut(s) 783
BspFNI CGCG 1 cut(s) 1318
BspLI GGNNCC 2 cut(s) 1281, 1312
BspMAI CTGCAG 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 5 cut(s) 15, 1010, 1110, 1274, 1287
BspQI GCTCTTC 1 cut(s) 1515
BsrDI GCAATG 2 cut(s) 436, 573
BsrGI TGTACA 2 cut(s) 713, 1505
BsrI ACTGG 6 cut(s) 18, 432, 594, 956, 966, 1306
BssECI CCNNGG 1 cut(s) 1169
BssMI GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
BssSI CACGAG 1 cut(s) 1675
BssT1I CCWWGG 1 cut(s) 1169
Bst2BI CACGAG 1 cut(s) 1675
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 5 cut(s) 668, 886, 975, 1510, 1570
Bst6I CTCTTC 1 cut(s) 1515
BstAPI GCANNNNNTGC 2 cut(s) 428, 1478
BstAUI TGTACA 2 cut(s) 713, 1505
BstC8I GCNNGC 5 cut(s) 420, 873, 1470, 1483, 1487
BstDEI CTNAG 5 cut(s) 117, 474, 1098, 1500, 1650
BstF5I GGATG 5 cut(s) 475, 757, 794, 945, 1165
BstFNI CGCG 1 cut(s) 1318
BstKTI GATC 7 cut(s) 10, 216, 746, 1018, 1105, 1282, 1345
BstMAI GTCTC 2 cut(s) 230, 624
BstMBI GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
BstMWI GCNNNNNNNGC 6 cut(s) 428, 850, 1324, 1478, 1580, 1693
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 3 cut(s) 398, 1032, 1489
BstSCI CCNGG 1 cut(s) 505
BstSFI CTRYAG 2 cut(s) 109, 842
BstSLI GKGCMC 1 cut(s) 1150
BstUI CGCG 1 cut(s) 1318
BstV1I GCAGC 7 cut(s) 92, 95, 828, 856, 1226, 1311, 1429
BstV2I GAAGAC 1 cut(s) 1695
BstX2I RGATCY 4 cut(s) 743, 1102, 1279, 1342
BstYI RGATCY 4 cut(s) 743, 1102, 1279, 1342
Bsu15I ATCGAT 1 cut(s) 783
BsuI GTATCC 1 cut(s) 986
BsuRI GGCC 2 cut(s) 563, 693
BsuTUI ATCGAT 1 cut(s) 783
BtrI CACGTC 2 cut(s) 187, 1151
BtsCI GGATG 5 cut(s) 475, 757, 794, 945, 1165
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 2 cut(s) 728, 959
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 5 cut(s) 420, 873, 1470, 1483, 1487
Cfr13I GGNCC 1 cut(s) 1310
ClaI ATCGAT 1 cut(s) 783
Csp6I GTAC 5 cut(s) 200, 587, 714, 1460, 1506
CviAII CATG 9 cut(s) 247, 346, 395, 517, 673, 734, 1029, 1486, 1646
CviQI GTAC 5 cut(s) 200, 587, 714, 1460, 1506
DdeI CTNAG 5 cut(s) 117, 474, 1098, 1500, 1650
DpnI GATC 7 cut(s) 9, 215, 745, 1017, 1104, 1281, 1344
DpnII GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
DraI TTTAAA 1 cut(s) 1062
DraIII CACNNNGTG 1 cut(s) 584
DriI GACNNNNNGTC 1 cut(s) 955
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 1515
Eam1105I GACNNNNNGTC 1 cut(s) 955
EarI CTCTTC 1 cut(s) 1515
Ecl136II GAGCTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 1169
Eco24I GRGCYC 1 cut(s) 118
Eco32I GATATC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 1310
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 1416
EcoICRI GAGCTC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 1310
EcoRI GAATTC 1 cut(s) 1424
EcoRII CCWGG 1 cut(s) 505
EcoRV GATATC 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 1169
EcoT38I GRGCYC 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 1169
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 9 cut(s) 250, 349, 398, 520, 676, 737, 1032, 1489, 1649
FaqI GGGAC 3 cut(s) 904, 1205, 1579
FatI CATG 9 cut(s) 246, 345, 394, 516, 672, 733, 1028, 1485, 1645
FbaI TGATCA 1 cut(s) 213
FblI GTMKAC 1 cut(s) 1656
Fnu4HI GCNGC 9 cut(s) 81, 84, 842, 845, 1215, 1325, 1443, 1490, 1584
FokI GGATG 5 cut(s) 482, 764, 801, 952, 1152
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 9 cut(s) 81, 84, 842, 845, 1215, 1325, 1443, 1490, 1584
FspBI CTAG 1 cut(s) 656
GluI GCNGC 9 cut(s) 81, 84, 842, 845, 1215, 1325, 1443, 1490, 1584
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 2 cut(s) 563, 693
HapII CCGG 1 cut(s) 1286
Hin1II CATG 9 cut(s) 250, 349, 398, 520, 676, 737, 1032, 1489, 1649
HincII GTYRAC 2 cut(s) 277, 1657
HindII GTYRAC 2 cut(s) 277, 1657
HindIII AAGCTT 1 cut(s) 1401
HinfI GANTC 3 cut(s) 523, 599, 1333
HpaII CCGG 1 cut(s) 1286
HphI GGTGA 4 cut(s) 524, 1564, 1613, 1643
Hpy166II GTNNAC 5 cut(s) 277, 587, 1148, 1506, 1657
Hpy188I TCNGA 6 cut(s) 120, 184, 367, 609, 685, 1012
Hpy188III TCNNGA 8 cut(s) 318, 467, 656, 707, 797, 833, 1070, 1308
Hpy8I GTNNAC 5 cut(s) 277, 587, 1148, 1506, 1657
Hpy99I CGWCG 1 cut(s) 1661
HpyAV CCTTC 7 cut(s) 17, 23, 29, 67, 659, 922, 1503
HpyCH4III ACNGT 5 cut(s) 668, 886, 975, 1510, 1570
HpyCH4IV ACGT 3 cut(s) 186, 1150, 1641
HpyF10VI GCNNNNNNNGC 6 cut(s) 428, 850, 1324, 1478, 1580, 1693
HpyF3I CTNAG 5 cut(s) 117, 474, 1098, 1500, 1650
HpySE526I ACGT 3 cut(s) 186, 1150, 1641
Hsp92II CATG 9 cut(s) 250, 349, 398, 520, 676, 737, 1032, 1489, 1649
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
LguI GCTCTTC 1 cut(s) 1515
LmnI GCTCC 2 cut(s) 965, 1240
Lsp1109I GCAGC 7 cut(s) 92, 95, 828, 856, 1226, 1311, 1429
LweI GCATC 7 cut(s) 460, 790, 1066, 1298, 1369, 1402, 1481
MaeI CTAG 1 cut(s) 656
MaeII ACGT 3 cut(s) 186, 1150, 1641
MaeIII GTNAC 5 cut(s) 512, 668, 947, 957, 1601
MalI GATC 7 cut(s) 9, 215, 745, 1017, 1104, 1281, 1344
MboI GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
MboII GAAGA 6 cut(s) 136, 370, 644, 1085, 1532, 1695
MfeI CAATTG 1 cut(s) 1328
MflI RGATCY 4 cut(s) 743, 1102, 1279, 1342
MhlI GDGCHC 2 cut(s) 118, 1150
MlsI TGGCCA 1 cut(s) 563
MluCI AATT 6 cut(s) 170, 566, 760, 1064, 1328, 1424
MluNI TGGCCA 1 cut(s) 563
MlyI GAGTC 2 cut(s) 517, 1327
MmeI TCCRAC 2 cut(s) 800, 970
Mox20I TGGCCA 1 cut(s) 563
MroXI GAANNNNTTC 3 cut(s) 603, 1303, 1524
MscI TGGCCA 1 cut(s) 563
MseI TTAA 7 cut(s) 255, 447, 488, 548, 636, 923, 1061
MslI CAYNNNNRTG 1 cut(s) 1501
Msp20I TGGCCA 1 cut(s) 563
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 1 cut(s) 1286
MspR9I CCNGG 1 cut(s) 507
MunI CAATTG 1 cut(s) 1328
Mva1269I GAATGC 1 cut(s) 1217
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 1318
MwoI GCNNNNNNNGC 6 cut(s) 428, 850, 1324, 1478, 1580, 1693
NdeII GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
NlaIII CATG 9 cut(s) 250, 349, 398, 520, 676, 737, 1032, 1489, 1649
NlaIV GGNNCC 2 cut(s) 1281, 1312
NmuCI GTSAC 5 cut(s) 512, 668, 947, 957, 1601
NspI RCATGY 3 cut(s) 398, 1032, 1489
PaeI GCATGC 1 cut(s) 1489
PaeR7I CTCGAG 1 cut(s) 1416
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 1515
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 1 cut(s) 1217
PdmI GAANNNNTTC 3 cut(s) 603, 1303, 1524
PfeI GAWTC 1 cut(s) 599
PkrI GCNGC 9 cut(s) 82, 85, 843, 846, 1216, 1326, 1444, 1491, 1585
PleI GAGTC 2 cut(s) 517, 1327
PpsI GAGTC 2 cut(s) 517, 1327
PpuMI RGGWCCY 1 cut(s) 1310
PscI ACATGT 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 118
Psp1406I AACGTT 1 cut(s) 1641
Psp5II RGGWCCY 1 cut(s) 1310
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 1281, 1312
PspPI GGNCC 1 cut(s) 1310
PspPPI RGGWCCY 1 cut(s) 1310
PspXI VCTCGAGB 1 cut(s) 1416
PstI CTGCAG 1 cut(s) 846
PsuI RGATCY 4 cut(s) 743, 1102, 1279, 1342
PvuII CAGCTG 1 cut(s) 841
RsaI GTAC 5 cut(s) 201, 588, 715, 1461, 1507
RsaNI GTAC 5 cut(s) 200, 587, 714, 1460, 1506
RseI CAYNNNNRTG 1 cut(s) 1501
SacI GAGCTC 1 cut(s) 118
SalI GTCGAC 1 cut(s) 1655
SapI GCTCTTC 1 cut(s) 1515
SaqAI TTAA 7 cut(s) 255, 447, 488, 548, 636, 923, 1061
SatI GCNGC 9 cut(s) 81, 84, 842, 845, 1215, 1325, 1443, 1490, 1584
Sau3AI GATC 7 cut(s) 7, 213, 743, 1015, 1102, 1279, 1342
Sau96I GGNCC 1 cut(s) 1310
SchI GAGTC 2 cut(s) 517, 1327
ScrFI CCNGG 1 cut(s) 507
SduI GDGCHC 2 cut(s) 118, 1150
SfaNI GCATC 7 cut(s) 460, 790, 1066, 1298, 1369, 1402, 1481
SfcI CTRYAG 2 cut(s) 109, 842
Sfr274I CTCGAG 1 cut(s) 1416
SinI GGWCC 1 cut(s) 1310
SlaI CTCGAG 1 cut(s) 1416
SmiMI CAYNNNNRTG 1 cut(s) 1501
SmlI CTYRAG 2 cut(s) 1091, 1416
SmoI CTYRAG 2 cut(s) 1091, 1416
SphI GCATGC 1 cut(s) 1489
Sse9I AATT 6 cut(s) 170, 566, 760, 1064, 1328, 1424
SsiI CCGC 7 cut(s) 803, 1318, 1490, 1550, 1584, 1694, 1717
SspMI CTAG 1 cut(s) 656
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 1 cut(s) 1169
TaaI ACNGT 5 cut(s) 668, 886, 975, 1510, 1570
TaiI ACGT 3 cut(s) 189, 1153, 1644
TaqI TCGA 8 cut(s) 10, 56, 308, 783, 881, 1417, 1656, 1706
TasI AATT 6 cut(s) 170, 566, 760, 1064, 1328, 1424
TatI WGTACW 3 cut(s) 586, 713, 1505
TauI GCSGC 2 cut(s) 1492, 1586
TfiI GAWTC 1 cut(s) 599
Tru1I TTAA 7 cut(s) 255, 447, 488, 548, 636, 923, 1061
Tru9I TTAA 7 cut(s) 255, 447, 488, 548, 636, 923, 1061
TscAI CASTG 2 cut(s) 735, 966
TseFI GTSAC 5 cut(s) 512, 668, 947, 957, 1601
TseI GCWGC 7 cut(s) 80, 83, 841, 844, 1214, 1324, 1442
Tsp45I GTSAC 5 cut(s) 512, 668, 947, 957, 1601
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 2 cut(s) 735, 966
VneI GTGCAC 1 cut(s) 1146
VpaK11BI GGWCC 1 cut(s) 1310
XapI RAATTY 4 cut(s) 566, 760, 1064, 1424
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 3 cut(s) 398, 1032, 1489
XhoI CTCGAG 1 cut(s) 1416
XmiI GTMKAC 1 cut(s) 1656
XmnI GAANNNNTTC 3 cut(s) 603, 1303, 1524
XspI CTAG 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.