Prupe.1G408200_v2.0.a1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010799
Physical Location & Seq
Reverse (-)
35757921 .. 35764246
6326 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G408200.1

Sequence Viewer

Length: 1515 bp
ATGGAGGATCGAGAGGAGCAGGAAGAGGAGGTGTTGGGGTCAAGCTTGACGATGGAGAAGGTGGCAGCAGCCAAACAGTTCATAGAGAACCACTACAGAGCTCAGATGAAGAACATTCAAGAACGAAAAGAGAGACGATGGGTGTTGGAAAGGAAATTAGCTTCCTCAGATGTGCCAAAGGAGGTACAAGTCAACCTGATCAAAGACTTAGAGCGAAAGGAAACAGAATTCATGCGACTTAAAAGGCACAAGATTTGTGTTGATGATTTTGAACCTTTGACCATCATTGGTAGGGGGGCCTTTGGTGAGGTCCGATTGTGTCGAGAGAAAAAATCTGGCAATATCTATGCCATGAAAAAGTTGAAGAAATCTGAAATGCTTAAGAGAGGACAGGTGGAACATGTTAGAGCTGAAAGAAACTTGCTGGCTGAAGTTGCCAGCCATTGCATAGTTAAACTTTACTACTCCTTTCAGGATGCTGAGTACTTATATTTAATAATGGAATATCTGCCCGGTGGTGACATGATGACTCTGCTGATGAGGGAAGACACCTTAACTGAAAGTGTGGCTAAATTTTACATTGCACAGAGTGTACTGGCCATTGAATCTATCCAGAAACACAACTATGTACACAGAGACATTAAACCAGACAACCTTCTTCTGGATAAAAATGGTCACATGAAACTCTCGGATTTTGGCCTCTGTAAGCCTCTTGATTGTACAACTTTACCTTCACTGCATGAAAATAGAACTATGGATGATGAAAATTTGACAGAACCAATGGATATCGACGTATGCGTTCCTGATGCGGATAATAGGAGTAGTTGGAAAAGCCCCCGTGAACAGCTGCAGCATTGGCAGATGAACAGGAGGAAGTTGGCATTTTCAACTGTGGGGACCCCCGACTATATTGCACCTGAAGTGCTATTGAAGAAAGGATATGGAATGGAATGCGACTGGTGGTCATTGGGAGCAATAATGTATGAAATGCTTGTTGGTTACCCTCCATTTTATTCAGATGATCCAATAACTACATGCAGAAAGATTGTTCATTGGAGAAACCACTTAAAATTTCCAGAAGATGCAAGGTTGACAGCTGAGGCTAAGGATCTTATTTGCAGGTTGCTCTGTGATGTTGAATATCGGCTAGGTACTGGTGGCGCACATCAAATCAAATCTCATCCTTGGTTCAAGAACATTGTGTGGGACAAACTCTATGAAATGGAGGCAGCATTCAAACCAGAAGTGAATGGGGAACTTGATACCCAGAACTTTATGAAGTTTGACGAGTTGGAGCCTCCAGTGTCATCAAGAACTGGCTCAGGACCCTCACGAAAGCTGCAATTGACTCCCAAAGATCTAAGCTTTGTTGGCTATACATACAAGAACTTTGATGCTGTAAAAGGGCTTCGCCAAGTTTTTGGTGATTCAAGAGTAGATTTTACTAGTGAACGGGCAGCCAAGGAAGCAGAGTTACATATGCTTGCATCATCTGGTGATCCCATGTTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

505

Amino Acids

58.4

Weight (kDa)

6.12

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33080 AT4G33080
fragaria_vesca FvH4_2g31460 FvH4_2g31460
malus_domestica MD08G1066200.v1.1 MD15G1055000.v1.1
prunus_persica Prupe.1G408200_v2.0.a1
pyrus_communis pycom08g05490 pycom15g05190
rosa_chinensis RchiOBHm_Chr6g0311341
rosa_laevigata RLG00000010415
rosa_multiflora Rmu_sc0008536.1_g000007
rosa_roxburghii Rroxscaffold_7G00157700
rosa_rugosa Rorug06G0387700 Rorug06G0387700
rosa_samantha Rh6AG502400 Rh6BG513000 Rh6CG518800 Rh6DG505000
rosa_wichuraiana Rw6G043750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1110
AciI CCGC 1 cut(s) 809
AclWI GGATC 4 cut(s) 15, 1016, 1116, 1493
AcoI YGGCCR 1 cut(s) 597
AcsI RAATTY 4 cut(s) 227, 572, 766, 1070
AcuI CTGAAG 2 cut(s) 450, 939
AdeI CACNNNGTG 1 cut(s) 590
AfaI GTAC 6 cut(s) 186, 485, 594, 630, 721, 1153
AfiI CCNNNNNNNGG 1 cut(s) 661
AflII CTTAAG 1 cut(s) 380
AflIII ACRYGT 1 cut(s) 400
AhdI GACNNNNNGTC 1 cut(s) 961
AhlI ACTAGT 1 cut(s) 1445
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
AluBI AGCT 8 cut(s) 45, 101, 161, 410, 847, 1097, 1339, 1365
AluI AGCT 8 cut(s) 45, 101, 161, 410, 847, 1097, 1339, 1365
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 2 cut(s) 127, 630
AlwI GGATC 4 cut(s) 15, 1016, 1116, 1493
AoxI GGCC 3 cut(s) 297, 597, 697
ApeKI GCWGC 7 cut(s) 65, 68, 847, 850, 1229, 1339, 1457
ApoI RAATTY 4 cut(s) 227, 572, 766, 1070
AspLEI GCGC 1 cut(s) 1163
AspS9I GGNCC 4 cut(s) 297, 310, 897, 1325
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 4 cut(s) 317, 530, 1436, 1508
AvaII GGWCC 3 cut(s) 310, 897, 1325
BalI TGGCCA 1 cut(s) 599
BanII GRGCYC 1 cut(s) 103
BarI GAAGNNNNNNTAC 4 cut(s) 1392, 1424, 1458, 1490
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 103
BbvCI CCTCAGC 1 cut(s) 1098
BbvI GCAGC 7 cut(s) 77, 80, 834, 862, 1241, 1326, 1469
BccI CCATC 3 cut(s) 46, 132, 290
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 2 cut(s) 127, 630
BcuI ACTAGT 1 cut(s) 1445
BfaI CTAG 2 cut(s) 1148, 1446
BfmI CTRYAG 2 cut(s) 94, 848
BfrI CTTAAG 1 cut(s) 380
BfuAI ACCTGC 1 cut(s) 1110
BglII AGATCT 1 cut(s) 1357
BisI GCNGC 7 cut(s) 66, 69, 848, 851, 1230, 1340, 1458
BlsI GCNGC 7 cut(s) 67, 70, 849, 852, 1231, 1341, 1459
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 3 cut(s) 310, 897, 1325
BmeRI GACNNNNNGTC 1 cut(s) 961
BmgT120I GGNCC 4 cut(s) 297, 310, 897, 1325
BmiI GGNNCC 5 cut(s) 298, 898, 899, 1296, 1327
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 5 cut(s) 466, 796, 1072, 1384, 1496
BpiI GAAGAC 1 cut(s) 552
BpmI CTGGAG 1 cut(s) 1284
Bpu10I CCTNAGC 3 cut(s) 1098, 1104, 1321
BpuMI CCSGG 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 1184, 1461
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse1I ACTGG 5 cut(s) 600, 962, 1159, 1301, 1321
Bse3DI GCAATG 2 cut(s) 442, 579
BseDI CCNNGG 2 cut(s) 1184, 1461
BseGI GGATG 3 cut(s) 481, 763, 1180
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMI GCAATG 2 cut(s) 442, 579
BseMII CTCAG 5 cut(s) 116, 180, 471, 1089, 1335
BseNI ACTGG 5 cut(s) 600, 962, 1159, 1301, 1321
BseRI GAGGAG 2 cut(s) 29, 41
BseXI GCAGC 7 cut(s) 77, 80, 834, 862, 1241, 1326, 1469
BshFI GGCC 3 cut(s) 299, 599, 699
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 513
BslFI GGGAC 2 cut(s) 910, 1220
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 2 cut(s) 127, 630
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 910, 1220
BsmI GAATGC 2 cut(s) 956, 1232
BsnI GGCC 3 cut(s) 299, 599, 699
Bsp1286I GDGCHC 1 cut(s) 103
Bsp1407I TGTACA 2 cut(s) 628, 719
Bsp143I GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
BspACI CCGC 1 cut(s) 809
BspANI GGCC 3 cut(s) 299, 599, 699
BspCNI CTCAG 5 cut(s) 115, 179, 472, 1090, 1334
BspLI GGNNCC 5 cut(s) 298, 898, 899, 1296, 1327
BspMAI CTGCAG 1 cut(s) 852
BspMI ACCTGC 1 cut(s) 1110
BspPI GGATC 4 cut(s) 15, 1016, 1116, 1493
BspTI CTTAAG 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 442, 579
BsrGI TGTACA 2 cut(s) 628, 719
BsrI ACTGG 5 cut(s) 600, 962, 1159, 1301, 1321
BssECI CCNNGG 2 cut(s) 1184, 1461
BssMI GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
BssT1I CCWWGG 2 cut(s) 1184, 1461
Bst4CI ACNGT 2 cut(s) 78, 892
Bst6I CTCTTC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 380
BstAUI TGTACA 2 cut(s) 628, 719
BstC8I GCNNGC 3 cut(s) 426, 439, 1485
BstDEI CTNAG 8 cut(s) 102, 166, 208, 480, 1098, 1104, 1321, 1361
BstEII GGTNACC 1 cut(s) 998
BstF5I GGATG 3 cut(s) 481, 763, 1180
BstHHI GCGC 1 cut(s) 1163
BstKTI GATC 6 cut(s) 10, 201, 1024, 1111, 1360, 1501
BstMAI GTCTC 2 cut(s) 127, 630
BstMBI GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
BstMWI GCNNNNNNNGC 4 cut(s) 434, 856, 1371, 1466
BstNSI RCATGY 2 cut(s) 404, 1038
BstPI GGTNACC 1 cut(s) 998
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 2 cut(s) 94, 848
BstV1I GCAGC 7 cut(s) 77, 80, 834, 862, 1241, 1326, 1469
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 2 cut(s) 1108, 1357
BstXI CCANNNNNNTGG 1 cut(s) 1421
BstYI RGATCY 2 cut(s) 1108, 1357
BsuRI GGCC 3 cut(s) 299, 599, 699
BtsCI GGATG 3 cut(s) 481, 763, 1180
BtsI GCAGTG 1 cut(s) 734
BtsIMutI CAGTG 2 cut(s) 734, 1308
BveI ACCTGC 1 cut(s) 1110
Cac8I GCNNGC 3 cut(s) 426, 439, 1485
CfoI GCGC 1 cut(s) 1163
Cfr13I GGNCC 4 cut(s) 297, 310, 897, 1325
Csp6I GTAC 6 cut(s) 185, 484, 593, 629, 720, 1152
CviAII CATG 8 cut(s) 232, 352, 401, 523, 679, 740, 1035, 1504
CviQI GTAC 6 cut(s) 185, 484, 593, 629, 720, 1152
DdeI CTNAG 8 cut(s) 102, 166, 208, 480, 1098, 1104, 1321, 1361
DpnI GATC 6 cut(s) 9, 200, 1023, 1110, 1359, 1500
DpnII GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
DraIII CACNNNGTG 1 cut(s) 590
DriI GACNNNNNGTC 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 597
Eam1104I CTCTTC 1 cut(s) 18
Eam1105I GACNNNNNGTC 1 cut(s) 961
EarI CTCTTC 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 1184, 1461
Eco24I GRGCYC 1 cut(s) 103
Eco32I GATATC 1 cut(s) 787
Eco47I GGWCC 3 cut(s) 310, 897, 1325
Eco53kI GAGCTC 1 cut(s) 101
Eco57I CTGAAG 2 cut(s) 450, 939
Eco91I GGTNACC 1 cut(s) 998
EcoICRI GAGCTC 1 cut(s) 101
EcoO109I RGGNCCY 3 cut(s) 297, 897, 1325
EcoO65I GGTNACC 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 227
EcoRV GATATC 1 cut(s) 787
EcoT14I CCWWGG 2 cut(s) 1184, 1461
EcoT38I GRGCYC 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 1184, 1461
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 8 cut(s) 235, 355, 404, 526, 682, 743, 1038, 1507
FaqI GGGAC 2 cut(s) 910, 1220
FatI CATG 8 cut(s) 231, 351, 400, 522, 678, 739, 1034, 1503
FauNDI CATATG 1 cut(s) 1479
FbaI TGATCA 1 cut(s) 198
Fnu4HI GCNGC 7 cut(s) 66, 69, 848, 851, 1230, 1340, 1458
FokI GGATG 3 cut(s) 488, 770, 1167
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 7 cut(s) 66, 69, 848, 851, 1230, 1340, 1458
FspBI CTAG 2 cut(s) 1148, 1446
GlaI GCGC 1 cut(s) 1162
GluI GCNGC 7 cut(s) 66, 69, 848, 851, 1230, 1340, 1458
GsuI CTGGAG 1 cut(s) 1284
HaeIII GGCC 3 cut(s) 299, 599, 699
HapII CCGG 1 cut(s) 513
HhaI GCGC 1 cut(s) 1163
Hin1II CATG 8 cut(s) 235, 355, 404, 526, 682, 743, 1038, 1507
Hin6I GCGC 1 cut(s) 1161
HinP1I GCGC 1 cut(s) 1161
HincII GTYRAC 2 cut(s) 193, 1092
HindII GTYRAC 2 cut(s) 193, 1092
HindIII AAGCTT 2 cut(s) 43, 1363
HinfI GANTC 4 cut(s) 529, 605, 1348, 1427
HpaII CCGG 1 cut(s) 513
HphI GGTGA 4 cut(s) 317, 530, 1436, 1508
Hpy166II GTNNAC 6 cut(s) 193, 593, 631, 842, 1092, 1451
Hpy188I TCNGA 6 cut(s) 105, 169, 314, 373, 691, 1018
Hpy8I GTNNAC 6 cut(s) 193, 593, 631, 842, 1092, 1451
Hpy99I CGWCG 1 cut(s) 794
HpyAV CCTTC 3 cut(s) 52, 665, 741
HpyCH4III ACNGT 2 cut(s) 78, 892
HpyCH4IV ACGT 1 cut(s) 792
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 856, 1371, 1466
HpyF3I CTNAG 8 cut(s) 102, 166, 208, 480, 1098, 1104, 1321, 1361
HpySE526I ACGT 1 cut(s) 792
Hsp92II CATG 8 cut(s) 235, 355, 404, 526, 682, 743, 1038, 1507
HspAI GCGC 1 cut(s) 1161
KflI GGGWCCC 1 cut(s) 897
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
LmnI GCTCC 3 cut(s) 16, 971, 1294
Lsp1109I GCAGC 7 cut(s) 77, 80, 834, 862, 1241, 1326, 1469
LweI GCATC 5 cut(s) 466, 796, 1072, 1384, 1496
MaeI CTAG 2 cut(s) 1148, 1446
MaeII ACGT 1 cut(s) 792
MaeIII GTNAC 5 cut(s) 518, 674, 998, 1473, 1506
MalI GATC 6 cut(s) 9, 200, 1023, 1110, 1359, 1500
MboI GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
MboII GAAGA 7 cut(s) 35, 121, 376, 557, 650, 943, 1091
MfeI CAATTG 1 cut(s) 1343
MflI RGATCY 2 cut(s) 1108, 1357
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 599
MluCI AATT 6 cut(s) 155, 227, 572, 766, 1070, 1343
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 2 cut(s) 523, 1342
MmeI TCCRAC 3 cut(s) 126, 806, 1272
Mox20I TGGCCA 1 cut(s) 599
MscI TGGCCA 1 cut(s) 599
MseI TTAA 7 cut(s) 240, 381, 453, 494, 554, 642, 1067
MslI CAYNNNNRTG 1 cut(s) 624
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 2 cut(s) 847, 1097
MspCI CTTAAG 1 cut(s) 380
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 1 cut(s) 513
MunI CAATTG 1 cut(s) 1343
Mva1269I GAATGC 2 cut(s) 956, 1232
MwoI GCNNNNNNNGC 4 cut(s) 434, 856, 1371, 1466
NciI CCSGG 1 cut(s) 513
NdeI CATATG 1 cut(s) 1479
NdeII GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
NlaIII CATG 8 cut(s) 235, 355, 404, 526, 682, 743, 1038, 1507
NlaIV GGNNCC 5 cut(s) 298, 898, 899, 1296, 1327
NmuCI GTSAC 2 cut(s) 518, 674
NspI RCATGY 2 cut(s) 404, 1038
PciI ACATGT 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 2 cut(s) 956, 1232
PfeI GAWTC 2 cut(s) 605, 1427
PkrI GCNGC 7 cut(s) 67, 70, 849, 852, 1231, 1341, 1459
PleI GAGTC 2 cut(s) 523, 1342
PpsI GAGTC 2 cut(s) 523, 1342
PpuMI RGGWCCY 2 cut(s) 897, 1325
PscI ACATGT 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 103
Psp5II RGGWCCY 2 cut(s) 897, 1325
PspEI GGTNACC 1 cut(s) 998
PspN4I GGNNCC 5 cut(s) 298, 898, 899, 1296, 1327
PspPI GGNCC 4 cut(s) 297, 310, 897, 1325
PspPPI RGGWCCY 2 cut(s) 897, 1325
PstI CTGCAG 1 cut(s) 852
PsuI RGATCY 2 cut(s) 1108, 1357
PvuII CAGCTG 2 cut(s) 847, 1097
RsaI GTAC 6 cut(s) 186, 485, 594, 630, 721, 1153
RsaNI GTAC 6 cut(s) 185, 484, 593, 629, 720, 1152
RseI CAYNNNNRTG 1 cut(s) 624
SacI GAGCTC 1 cut(s) 103
SaqAI TTAA 7 cut(s) 240, 381, 453, 494, 554, 642, 1067
SatI GCNGC 7 cut(s) 66, 69, 848, 851, 1230, 1340, 1458
Sau3AI GATC 6 cut(s) 7, 198, 1021, 1108, 1357, 1498
Sau96I GGNCC 4 cut(s) 297, 310, 897, 1325
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 2 cut(s) 523, 1342
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 103
SfaNI GCATC 5 cut(s) 466, 796, 1072, 1384, 1496
SfcI CTRYAG 2 cut(s) 94, 848
SinI GGWCC 3 cut(s) 310, 897, 1325
SmiMI CAYNNNNRTG 1 cut(s) 624
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SpeI ACTAGT 1 cut(s) 1445
Sse9I AATT 6 cut(s) 155, 227, 572, 766, 1070, 1343
SsiI CCGC 1 cut(s) 809
SspMI CTAG 2 cut(s) 1148, 1446
SstI GAGCTC 1 cut(s) 103
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 2 cut(s) 1184, 1461
TaaI ACNGT 2 cut(s) 78, 892
TaiI ACGT 1 cut(s) 795
TaqI TCGA 3 cut(s) 10, 322, 789
TasI AATT 6 cut(s) 155, 227, 572, 766, 1070, 1343
TatI WGTACW 4 cut(s) 483, 592, 628, 719
TfiI GAWTC 2 cut(s) 605, 1427
Tru1I TTAA 7 cut(s) 240, 381, 453, 494, 554, 642, 1067
Tru9I TTAA 7 cut(s) 240, 381, 453, 494, 554, 642, 1067
TscAI CASTG 2 cut(s) 741, 1308
TseFI GTSAC 2 cut(s) 518, 674
TseI GCWGC 7 cut(s) 65, 68, 847, 850, 1229, 1339, 1457
Tsp45I GTSAC 2 cut(s) 518, 674
TspRI CASTG 2 cut(s) 741, 1308
Vha464I CTTAAG 1 cut(s) 380
VpaK11BI GGWCC 3 cut(s) 310, 897, 1325
XapI RAATTY 4 cut(s) 227, 572, 766, 1070
XceI RCATGY 2 cut(s) 404, 1038
XspI CTAG 2 cut(s) 1148, 1446
ZrmI AGTACT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.