Rh6BG513000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0010799
Physical Location & Seq
Forward (+)
70834272 .. 70840549
6278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG513000.1

Sequence Viewer

Length: 1275 bp
ATGCGACTTAAAAGGCATAGGATATGTGTTGACGATTTTGAGCCTTTGACCATCATTGGTAGGGGGGCTTTTGGTGAGGTTCGATTGTGTCGGGAGAAAAAATCTGGCAATATATATGCCATGAAAAAGTTGAAGAAATCTGAAATGCTTATGAGAGGACAGGTGGAACATGTTAGAGCTGAAAGAAACTTGCTGGCTGAAGTTGCCAGTCATTGCATAGTTAAACTTTACTACTCCTTTCAGGATGCTGAGTATTTATATTTAATAATGGAATATCTGCCTGGTGGTGACATGATGACTCTGCTGATGAGGGAGGACACCTTAAATGAAAGTGTGGCCAAATTTTACATTGCACAGAGTGTACTGGCAATAGAATCTATTCAGAAACACAACTACATACACAGAGACATTAAACCCGACAACCTTCTTCTAGATAAAAACGGTCACATGAAACTCTCGGATTTTGGCCTCTGTAAGCCTCTTGATTGTACAACTTTACCTGCACTGCATGAAAATAGATCTATGGATGATGAAAATTCAGCAGAACCAATGGATATCGATGGATGTTTTCCTGATGCGGATAACAGGAATAGTTGGAAAAGCCCCCTTGAACAGCTGCAGCATTGGCAGATGAACAGGAGGAAGCTGGCATTTTCGACTGTGGGGACACCCGACTATATTGCACCTGAAGTGTTATTAAAGAAGGGATATGGGATGGAATGCGACTGGTGGTCACTGGGAGCAATACTGTATGAAATGCTTGTTGGATACCCTCCATTTTATTCAGATGATCCCATAACTACATGCAGAAAGATTGTTCATTGGAGAAATCATTTAAAATTTCCAGAAGATGCAAGGCTGACACTCGAGGCTAAGGATCTCATTTGCAGGTTGCTGTGTGATGTTGAACATAGGCTTGGTGCACGTCAAATCAAATCTCATCCTTGGTTCAAGGACATTGTGTGGGACAAACTTTATGAAATGGAGGCAGCATTCAAACCAGAAGTGAATGGGGAGCTTGATACCCAAAACTTTATGAAGTTTGATGAGCTGGATCCTCCAGCATCAGCAAGAACTGGTTCAGGACCCTCGCGGAAGCTGCAATTGACTCCCAAAGATCTAAACTTTGTTGGCTATACATACAAGAACTTTGATGCTGTAAAAGGGCTTCGCCAAGCTTTTGCTGATGCTCGAGTAGAATTCACTACTGAACGGGCAGCAAGAGAAACAGAGGTACAAATGCTTGCATCATCTGGTGATCCTATGTTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

424

Amino Acids

48.94

Weight (kDa)

6.1

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 14 - 162 3.7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 161 2.2e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 87 - 163 8.9e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 211 - 317 1.9e-22 Protein kinase domain
Pkinase_C PF00433 336 - 381 1.2e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33080 AT4G33080
fragaria_vesca FvH4_2g31460 FvH4_2g31460
malus_domestica MD08G1066200.v1.1 MD15G1055000.v1.1
prunus_persica Prupe.1G408200_v2.0.a1
pyrus_communis pycom08g05490 pycom15g05190
rosa_chinensis RchiOBHm_Chr6g0311341
rosa_laevigata RLG00000010415
rosa_multiflora Rmu_sc0008536.1_g000007
rosa_roxburghii Rroxscaffold_7G00157700
rosa_rugosa Rorug06G0387700 Rorug06G0387700
rosa_samantha Rh6AG502400 Rh6BG513000 Rh6CG518800 Rh6DG505000
rosa_wichuraiana Rw6G043750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 508, 879
AccII CGCG 1 cut(s) 1093
AciI CCGC 2 cut(s) 578, 1093
AclWI GGATC 5 cut(s) 785, 885, 1049, 1062, 1253
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 4 cut(s) 341, 535, 839, 1199
AcuI CTGAAG 2 cut(s) 219, 708
AdeI CACNNNGTG 1 cut(s) 359
AfaI GTAC 3 cut(s) 363, 490, 1236
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 5 cut(s) 133, 611, 908, 952, 997
AhdI GACNNNNNGTC 1 cut(s) 730
AjiI CACGTC 1 cut(s) 926
AjnI CCWGG 1 cut(s) 280
AjuI GAANNNNNNNTTGG 4 cut(s) 747, 779, 900, 932
AluBI AGCT 7 cut(s) 179, 616, 646, 1018, 1051, 1099, 1178
AluI AGCT 7 cut(s) 179, 616, 646, 1018, 1051, 1099, 1178
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 1 cut(s) 399
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 5 cut(s) 785, 885, 1049, 1062, 1253
Ama87I CYCGRG 2 cut(s) 866, 1191
AoxI GGCC 2 cut(s) 336, 466
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 5 cut(s) 616, 619, 989, 1099, 1217
ApoI RAATTY 4 cut(s) 341, 535, 839, 1199
Asp700I GAANNNNTTC 2 cut(s) 378, 1078
AspS9I GGNCC 1 cut(s) 1085
AsuHPI GGTGA 3 cut(s) 86, 299, 1268
AvaI CYCGRG 2 cut(s) 866, 1191
AvaII GGWCC 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 925
BalI TGGCCA 1 cut(s) 338
BamHI GGATCC 1 cut(s) 1054
BarI GAAGNNNNNNTAC 2 cut(s) 1152, 1184
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 5 cut(s) 603, 631, 1001, 1086, 1229
BccI CCATC 3 cut(s) 59, 554, 709
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 1 cut(s) 282
BciVI GTATCC 1 cut(s) 761
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 1 cut(s) 617
BfuAI ACCTGC 2 cut(s) 508, 879
BfuI GTATCC 1 cut(s) 761
BglII AGATCT 2 cut(s) 518, 1117
BisI GCNGC 5 cut(s) 617, 620, 990, 1100, 1218
BlsI GCNGC 5 cut(s) 618, 621, 991, 1101, 1219
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 1085
BmeRI GACNNNNNGTC 1 cut(s) 730
BmeT110I CYCGRG 2 cut(s) 866, 1191
BmgBI CACGTC 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 1085
BmiI GGNNCC 2 cut(s) 1056, 1087
BmrFI CCNGG 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 746
BmsI GCATC 7 cut(s) 235, 565, 841, 1073, 1144, 1177, 1256
BmuI ACTGGG 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 873
Bsa29I ATCGAT 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 944
Bse1I ACTGG 5 cut(s) 207, 369, 731, 741, 1081
Bse3DI GCAATG 2 cut(s) 211, 348
BseBI CCWGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 944
BseGI GGATG 5 cut(s) 250, 532, 569, 720, 940
BseMI GCAATG 2 cut(s) 211, 348
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 5 cut(s) 207, 369, 731, 741, 1081
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 5 cut(s) 603, 631, 1001, 1086, 1229
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 1093
BshFI GGCC 2 cut(s) 338, 468
BshVI ATCGAT 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 925
BsiHKCI CYCGRG 2 cut(s) 866, 1191
BslFI GGGAC 2 cut(s) 679, 980
BsmAI GTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 679, 980
BsmI GAATGC 2 cut(s) 725, 992
BsnI GGCC 2 cut(s) 338, 468
BsoBI CYCGRG 2 cut(s) 866, 1191
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 488
Bsp143I GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
BspACI CCGC 2 cut(s) 578, 1093
BspANI GGCC 2 cut(s) 338, 468
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 1 cut(s) 1093
BspLI GGNNCC 2 cut(s) 1056, 1087
BspMAI CTGCAG 1 cut(s) 621
BspMI ACCTGC 2 cut(s) 508, 879
BspPI GGATC 5 cut(s) 785, 885, 1049, 1062, 1253
BsrDI GCAATG 2 cut(s) 211, 348
BsrGI TGTACA 1 cut(s) 488
BsrI ACTGG 5 cut(s) 207, 369, 731, 741, 1081
BssECI CCNNGG 1 cut(s) 944
BssMI GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
BssT1I CCWWGG 1 cut(s) 944
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 3 cut(s) 443, 661, 750
BstAUI TGTACA 1 cut(s) 488
BstC8I GCNNGC 3 cut(s) 195, 648, 1245
BstDEI CTNAG 2 cut(s) 249, 873
BstF5I GGATG 5 cut(s) 250, 532, 569, 720, 940
BstFNI CGCG 1 cut(s) 1093
BstKTI GATC 6 cut(s) 521, 793, 880, 1057, 1120, 1261
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 203, 625, 1099
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 2 cut(s) 173, 807
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 925
BstUI CGCG 1 cut(s) 1093
BstV1I GCAGC 5 cut(s) 603, 631, 1001, 1086, 1229
BstX2I RGATCY 4 cut(s) 518, 877, 1054, 1117
BstYI RGATCY 4 cut(s) 518, 877, 1054, 1117
Bsu15I ATCGAT 1 cut(s) 558
BsuI GTATCC 1 cut(s) 761
BsuRI GGCC 2 cut(s) 338, 468
BsuTUI ATCGAT 1 cut(s) 558
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 5 cut(s) 250, 532, 569, 720, 940
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 2 cut(s) 503, 734
BveI ACCTGC 2 cut(s) 508, 879
Cac8I GCNNGC 3 cut(s) 195, 648, 1245
Cfr13I GGNCC 1 cut(s) 1085
ClaI ATCGAT 1 cut(s) 558
Csp6I GTAC 3 cut(s) 362, 489, 1235
CviAII CATG 6 cut(s) 121, 170, 292, 448, 509, 804
CviQI GTAC 3 cut(s) 362, 489, 1235
DdeI CTNAG 2 cut(s) 249, 873
DpnI GATC 6 cut(s) 520, 792, 879, 1056, 1119, 1260
DpnII GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
DraI TTTAAA 1 cut(s) 837
DraIII CACNNNGTG 1 cut(s) 359
DriI GACNNNNNGTC 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 944
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 1085
Eco57I CTGAAG 2 cut(s) 219, 708
Eco88I CYCGRG 2 cut(s) 866, 1191
EcoO109I RGGNCCY 1 cut(s) 1085
EcoRI GAATTC 1 cut(s) 1199
EcoRII CCWGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 944
ErhI CCWWGG 1 cut(s) 944
FaeI CATG 6 cut(s) 124, 173, 295, 451, 512, 807
FaqI GGGAC 2 cut(s) 679, 980
FatI CATG 6 cut(s) 120, 169, 291, 447, 508, 803
Fnu4HI GCNGC 5 cut(s) 617, 620, 990, 1100, 1218
FokI GGATG 5 cut(s) 257, 539, 576, 727, 927
Fsp4HI GCNGC 5 cut(s) 617, 620, 990, 1100, 1218
FspBI CTAG 1 cut(s) 431
GluI GCNGC 5 cut(s) 617, 620, 990, 1100, 1218
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 2 cut(s) 338, 468
Hin1II CATG 6 cut(s) 124, 173, 295, 451, 512, 807
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 1176
HinfI GANTC 3 cut(s) 298, 374, 1108
HphI GGTGA 3 cut(s) 86, 299, 1268
Hpy166II GTNNAC 3 cut(s) 31, 362, 923
Hpy188I TCNGA 4 cut(s) 142, 384, 460, 787
Hpy188III TCNNGA 7 cut(s) 92, 242, 431, 482, 572, 845, 1083
Hpy8I GTNNAC 3 cut(s) 31, 362, 923
HpyAV CCTTC 2 cut(s) 434, 697
HpyCH4III ACNGT 3 cut(s) 443, 661, 750
HpyCH4IV ACGT 1 cut(s) 925
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 625, 1099
HpyF3I CTNAG 2 cut(s) 249, 873
HpySE526I ACGT 1 cut(s) 925
Hsp92II CATG 6 cut(s) 124, 173, 295, 451, 512, 807
Kzo9I GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
LmnI GCTCC 2 cut(s) 740, 1015
Lsp1109I GCAGC 5 cut(s) 603, 631, 1001, 1086, 1229
LweI GCATC 7 cut(s) 235, 565, 841, 1073, 1144, 1177, 1256
MaeI CTAG 1 cut(s) 431
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 4 cut(s) 287, 443, 732, 1266
MalI GATC 6 cut(s) 520, 792, 879, 1056, 1119, 1260
MboI GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
MboII GAAGA 3 cut(s) 145, 419, 860
MfeI CAATTG 1 cut(s) 1103
MflI RGATCY 4 cut(s) 518, 877, 1054, 1117
MhlI GDGCHC 1 cut(s) 925
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 5 cut(s) 341, 535, 839, 1103, 1199
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 2 cut(s) 292, 1102
MmeI TCCRAC 2 cut(s) 575, 745
Mox20I TGGCCA 1 cut(s) 338
MroXI GAANNNNTTC 2 cut(s) 378, 1078
MscI TGGCCA 1 cut(s) 338
MseI TTAA 7 cut(s) 9, 222, 263, 323, 411, 698, 836
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 616
MspR9I CCNGG 1 cut(s) 282
MunI CAATTG 1 cut(s) 1103
Mva1269I GAATGC 2 cut(s) 725, 992
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 1093
MwoI GCNNNNNNNGC 3 cut(s) 203, 625, 1099
NdeII GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
NlaIII CATG 6 cut(s) 124, 173, 295, 451, 512, 807
NlaIV GGNNCC 2 cut(s) 1056, 1087
NmuCI GTSAC 3 cut(s) 287, 443, 732
NspI RCATGY 2 cut(s) 173, 807
PaeR7I CTCGAG 2 cut(s) 866, 1191
PciI ACATGT 1 cut(s) 169
PctI GAATGC 2 cut(s) 725, 992
PdmI GAANNNNTTC 2 cut(s) 378, 1078
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 5 cut(s) 618, 621, 991, 1101, 1219
PleI GAGTC 2 cut(s) 292, 1102
PpsI GAGTC 2 cut(s) 292, 1102
PpuMI RGGWCCY 1 cut(s) 1085
PscI ACATGT 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 1085
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 1056, 1087
PspPI GGNCC 1 cut(s) 1085
PspPPI RGGWCCY 1 cut(s) 1085
PspXI VCTCGAGB 2 cut(s) 866, 1191
PstI CTGCAG 1 cut(s) 621
PsuI RGATCY 4 cut(s) 518, 877, 1054, 1117
PvuII CAGCTG 1 cut(s) 616
RsaI GTAC 3 cut(s) 363, 490, 1236
RsaNI GTAC 3 cut(s) 362, 489, 1235
SaqAI TTAA 7 cut(s) 9, 222, 263, 323, 411, 698, 836
SatI GCNGC 5 cut(s) 617, 620, 990, 1100, 1218
Sau3AI GATC 6 cut(s) 518, 790, 877, 1054, 1117, 1258
Sau96I GGNCC 1 cut(s) 1085
SchI GAGTC 2 cut(s) 292, 1102
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 7 cut(s) 235, 565, 841, 1073, 1144, 1177, 1256
SfcI CTRYAG 1 cut(s) 617
Sfr274I CTCGAG 2 cut(s) 866, 1191
SinI GGWCC 1 cut(s) 1085
SlaI CTCGAG 2 cut(s) 866, 1191
SmlI CTYRAG 2 cut(s) 866, 1191
SmoI CTYRAG 2 cut(s) 866, 1191
Sse9I AATT 5 cut(s) 341, 535, 839, 1103, 1199
SsiI CCGC 2 cut(s) 578, 1093
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 944
TaaI ACNGT 3 cut(s) 443, 661, 750
TaiI ACGT 1 cut(s) 928
TaqI TCGA 5 cut(s) 82, 558, 656, 867, 1192
TasI AATT 5 cut(s) 341, 535, 839, 1103, 1199
TatI WGTACW 2 cut(s) 361, 488
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 7 cut(s) 9, 222, 263, 323, 411, 698, 836
Tru9I TTAA 7 cut(s) 9, 222, 263, 323, 411, 698, 836
TscAI CASTG 2 cut(s) 510, 741
TseFI GTSAC 3 cut(s) 287, 443, 732
TseI GCWGC 5 cut(s) 616, 619, 989, 1099, 1217
Tsp45I GTSAC 3 cut(s) 287, 443, 732
TspRI CASTG 2 cut(s) 510, 741
VneI GTGCAC 1 cut(s) 921
VpaK11BI GGWCC 1 cut(s) 1085
XapI RAATTY 4 cut(s) 341, 535, 839, 1199
XbaI TCTAGA 1 cut(s) 430
XceI RCATGY 2 cut(s) 173, 807
XhoI CTCGAG 2 cut(s) 866, 1191
XmnI GAANNNNTTC 2 cut(s) 378, 1078
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.