MD15G1055000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010799
Physical Location & Seq
Reverse (-)
3727362 .. 3732241
4880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1055000.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGGAGGATCGGGAGGAGCAGGAGGAGGAGGAGGAGGAGGTGCTGGGGTCGAGCTTGACGATGGAGAAGGTGGCGGCGGCCAAGCAGTTCATAGAGAACCACTACAGAGCTCAGATGAAGAACATTCAGGAACGGAAGGAGAGACGCTGGGTGTTGGAAAGGAAATTAGCTTCGTCAGATGTGCCAAAGGAGGTACAAATCAACCTGATCAAAGACTTAGAGCAAAAAGAGACAGAATTTATGCGACTTAAAAGGCACAAGATATGTGTTGATGATTTTGAGCCTTTGACCATCATTGGTAGGGGGGCCTTTGGTGAGGTCCGATTGTGTCGGGAGAAAAAATCTGGCAATATCTACGCCATGAAAAAGTTGAAGAAATCTGAAATGCTTAAGAGAGGACAGGTGGAACATGTTAGAGCTGAAAGAAACTTGCTGGCAGAAGTTGCCAGCCATTGCATAGTTAAACTATACTACTCCTTCCAAGATGCAGAGTACTTATACTTAATCATGGAATATCTGCCTGGGGGTGACATGATGACTCTGCTGATTAGGGAAGACACCTTAACTGAAAGTGTGGCAAAATTTTACATTGCACAGAGCGTACTGGCCATAGAATCTATTCAGAAACACAACTACATACACAGAGACATTAAGCCAGACAACCTGCTTCTGGATAAAAATGGTCACATGAAACTCTCAGATTTTGGCCTCTGTAAGCCTCTTGACTGTACAACTTTACCTGCACTGCATGAAAATAGAACTATGGATGACGAAAATATGACAGAACCAATGGATATCGACACATGCGTTCCTGATGCGGATAATCGGAGTAGTTGGAAAAGCCCTGCTGAACAGCTGCAACATTGGCAGATGAACAGGAGAAAGTTGGCATTTTCAACTGTGGGGACACCCGACTACATTGCACCTGAAGTGTTATTGAAGAAAGGATATGGGATGGAATGCGACTGGTGGTCATTGGGAGCAATAATGTACGAAATGCTTGTTGGTTACCCTCCATTTTATTCAGATGATCCAATAACTACATGCAGAAAGATTGTTCATTGGAGAAACCACTTAAAGTTTCCAGAAGACGCAAGGTTGACAGCTGAGGCTAAGGATCTTATTTGCAGGTTGCTGTGTGATGTTGAACATAGGCTAGGTACTGGTGGGGCACTTCAAATCAAATCTCATCCTTGGTTCAAGGACGTTGTGTGGGACAAACTCTATGAAATGGACGCAGCATTCAAACCAGAAGTGAATGGGGAGCTTGATACCCAGAACTTTATGAAGTTTGATGAGTTGGACCCACCAGCGTCTACAAGAACTGGATCTTCACGAAAGTTACAAATGACTCCCAAAGATTTAAGCTTTGTTGGCTACACATACAAGAACTTTGATGCTGTGAAGGGGCTTCGCCAAGCTCTTGGTGATGCTAGAGTAGATTTTACTGCTGAAAGGGCAGCCAAGGAAGCCGAGTTACAGATGCTTGCATCAGCCGGTGATCCCATGTTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_C

Protein Analysis

506

Amino Acids

58.33

Weight (kDa)

5.62

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 94 - 242 1.5e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 98 - 241 1.9e-22 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 167 - 243 1.9e-06 ABC1 atypical kinase-like domain
Pkinase PF00069 291 - 400 1.6e-20 Protein kinase domain
Pkinase_C PF00433 419 - 462 4.2e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33080 AT4G33080
fragaria_vesca FvH4_2g31460 FvH4_2g31460
malus_domestica MD08G1066200.v1.1 MD15G1055000.v1.1
prunus_persica Prupe.1G408200_v2.0.a1
pyrus_communis pycom08g05490 pycom15g05190
rosa_chinensis RchiOBHm_Chr6g0311341
rosa_laevigata RLG00000010415
rosa_multiflora Rmu_sc0008536.1_g000007
rosa_roxburghii Rroxscaffold_7G00157700
rosa_rugosa Rorug06G0387700 Rorug06G0387700
rosa_samantha Rh6AG502400 Rh6BG513000 Rh6CG518800 Rh6DG505000
rosa_wichuraiana Rw6G043750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 672, 748, 1119
AccI GTMKAC 1 cut(s) 1316
AciI CCGC 3 cut(s) 74, 77, 818
AclWI GGATC 5 cut(s) 15, 1025, 1125, 1336, 1496
AcoI YGGCCR 2 cut(s) 78, 606
AcsI RAATTY 2 cut(s) 236, 581
AcuI CTGAAG 1 cut(s) 948
AfaI GTAC 6 cut(s) 195, 494, 603, 730, 992, 1162
AfiI CCNNNNNNNGG 1 cut(s) 670
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 7 cut(s) 373, 897, 940, 1148, 1178, 1201, 1246
AhdI GACNNNNNGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 520
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
AluBI AGCT 9 cut(s) 54, 110, 170, 419, 856, 1106, 1267, 1368, 1421
AluI AGCT 9 cut(s) 54, 110, 170, 419, 856, 1106, 1267, 1368, 1421
Alw21I GWGCWC 1 cut(s) 112
Alw26I GTCTC 3 cut(s) 136, 224, 639
AlwI GGATC 5 cut(s) 15, 1025, 1125, 1336, 1496
AoxI GGCC 4 cut(s) 78, 306, 606, 706
ApeKI GCWGC 3 cut(s) 856, 1238, 1460
ApoI RAATTY 2 cut(s) 236, 581
Asp700I GAANNNNTTC 1 cut(s) 618
AspS9I GGNCC 3 cut(s) 306, 319, 1303
AsuHPI GGTGA 4 cut(s) 326, 539, 1439, 1511
AvaII GGWCC 2 cut(s) 319, 1303
BaeGI GKGCMC 1 cut(s) 1174
BalI TGGCCA 1 cut(s) 608
BanII GRGCYC 1 cut(s) 112
BarI GAAGNNNNNNTAC 2 cut(s) 1461, 1493
BbsI GAAGAC 2 cut(s) 561, 1095
Bbv12I GWGCWC 1 cut(s) 112
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 3 cut(s) 843, 1250, 1472
BccI CCATC 3 cut(s) 55, 299, 949
BcgI CGANNNNNNTGC 2 cut(s) 902, 936
BciT130I CCWGG 1 cut(s) 522
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 3 cut(s) 136, 224, 639
BfaI CTAG 2 cut(s) 1157, 1434
BfmI CTRYAG 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 3 cut(s) 672, 748, 1119
BisI GCNGC 5 cut(s) 75, 78, 857, 1239, 1461
BlsI GCNGC 5 cut(s) 76, 79, 858, 1240, 1462
BmcAI AGTACT 1 cut(s) 494
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 2 cut(s) 319, 1303
BmeRI GACNNNNNGTC 1 cut(s) 970
BmgT120I GGNCC 3 cut(s) 306, 319, 1303
BmiI GGNNCC 2 cut(s) 307, 1305
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 6 cut(s) 475, 805, 1387, 1420, 1473, 1499
BpiI GAAGAC 2 cut(s) 561, 1095
Bpu10I CCTNAGC 2 cut(s) 1107, 1113
BsaJI CCNNGG 3 cut(s) 521, 1193, 1464
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse118I RCCGGY 1 cut(s) 1496
Bse1I ACTGG 4 cut(s) 609, 971, 1168, 1330
Bse3DI GCAATG 3 cut(s) 451, 588, 918
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 521, 1193, 1464
BseGI GGATG 3 cut(s) 772, 960, 1189
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMI GCAATG 3 cut(s) 451, 588, 918
BseMII CTCAG 3 cut(s) 125, 711, 1098
BseNI ACTGG 4 cut(s) 609, 971, 1168, 1330
BseRI GAGGAG 6 cut(s) 29, 38, 41, 44, 47, 50
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 3 cut(s) 843, 1250, 1472
BseYI CCCAGC 2 cut(s) 43, 147
BsgI GTGCAG 1 cut(s) 726
BshFI GGCC 4 cut(s) 80, 308, 608, 708
BsiHKAI GWGCWC 1 cut(s) 112
BsiSI CCGG 1 cut(s) 1497
BslFI GGGAC 2 cut(s) 919, 1229
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 3 cut(s) 136, 224, 639
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 2 cut(s) 919, 1229
BsmI GAATGC 2 cut(s) 965, 1241
BsnI GGCC 4 cut(s) 80, 308, 608, 708
Bsp1286I GDGCHC 2 cut(s) 112, 1174
Bsp1407I TGTACA 1 cut(s) 728
Bsp143I GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
BspACI CCGC 3 cut(s) 74, 77, 818
BspANI GGCC 4 cut(s) 80, 308, 608, 708
BspCNI CTCAG 3 cut(s) 124, 710, 1099
BspLI GGNNCC 2 cut(s) 307, 1305
BspMI ACCTGC 3 cut(s) 672, 748, 1119
BspPI GGATC 5 cut(s) 15, 1025, 1125, 1336, 1496
BspTI CTTAAG 1 cut(s) 389
BsrDI GCAATG 3 cut(s) 451, 588, 918
BsrFI RCCGGY 1 cut(s) 1496
BsrGI TGTACA 1 cut(s) 728
BsrI ACTGG 4 cut(s) 609, 971, 1168, 1330
BssAI RCCGGY 1 cut(s) 1496
BssECI CCNNGG 3 cut(s) 521, 1193, 1464
BssMI GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
BssT1I CCWWGG 2 cut(s) 1193, 1464
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 2 cut(s) 728, 901
BstAFI CTTAAG 1 cut(s) 389
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 728
BstC8I GCNNGC 3 cut(s) 435, 448, 1488
BstDEI CTNAG 5 cut(s) 111, 217, 697, 1107, 1113
BstEII GGTNACC 1 cut(s) 1007
BstF5I GGATG 3 cut(s) 772, 960, 1189
BstKTI GATC 6 cut(s) 10, 210, 1033, 1120, 1331, 1504
BstMAI GTCTC 3 cut(s) 136, 224, 639
BstMBI GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
BstMWI GCNNNNNNNGC 5 cut(s) 443, 865, 1374, 1457, 1469
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 3 cut(s) 413, 807, 1047
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 1 cut(s) 520
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 1174
BstV1I GCAGC 3 cut(s) 843, 1250, 1472
BstV2I GAAGAC 2 cut(s) 561, 1095
BstX2I RGATCY 2 cut(s) 1117, 1328
BstXI CCANNNNNNTGG 1 cut(s) 1424
BstYI RGATCY 2 cut(s) 1117, 1328
BsuRI GGCC 4 cut(s) 80, 308, 608, 708
BtsCI GGATG 3 cut(s) 772, 960, 1189
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 1 cut(s) 743
BveI ACCTGC 3 cut(s) 672, 748, 1119
Cac8I GCNNGC 3 cut(s) 435, 448, 1488
Cfr10I RCCGGY 1 cut(s) 1496
Cfr13I GGNCC 3 cut(s) 306, 319, 1303
CseI GACGC 4 cut(s) 153, 1100, 1244, 1302
Csp6I GTAC 6 cut(s) 194, 493, 602, 729, 991, 1161
CviAII CATG 9 cut(s) 361, 410, 508, 532, 688, 749, 804, 1044, 1507
CviQI GTAC 6 cut(s) 194, 493, 602, 729, 991, 1161
DdeI CTNAG 5 cut(s) 111, 217, 697, 1107, 1113
DpnI GATC 6 cut(s) 9, 209, 1032, 1119, 1330, 1503
DpnII GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
DriI GACNNNNNGTC 1 cut(s) 970
EaeI YGGCCR 2 cut(s) 78, 606
Eam1105I GACNNNNNGTC 1 cut(s) 970
Ecl136II GAGCTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 1193, 1464
Eco24I GRGCYC 1 cut(s) 112
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 2 cut(s) 319, 1303
Eco53kI GAGCTC 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 948
Eco91I GGTNACC 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 1007
EcoRII CCWGG 1 cut(s) 520
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 2 cut(s) 1193, 1464
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 1193, 1464
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 9 cut(s) 364, 413, 511, 535, 691, 752, 807, 1047, 1510
FaqI GGGAC 2 cut(s) 919, 1229
FatI CATG 9 cut(s) 360, 409, 507, 531, 687, 748, 803, 1043, 1506
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 1316
Fnu4HI GCNGC 5 cut(s) 75, 78, 857, 1239, 1461
FokI GGATG 3 cut(s) 779, 967, 1176
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 5 cut(s) 75, 78, 857, 1239, 1461
FspBI CTAG 2 cut(s) 1157, 1434
GluI GCNGC 5 cut(s) 75, 78, 857, 1239, 1461
GsaI CCCAGC 2 cut(s) 47, 151
HaeIII GGCC 4 cut(s) 80, 308, 608, 708
HapII CCGG 1 cut(s) 1497
HgaI GACGC 4 cut(s) 153, 1100, 1244, 1302
Hin1II CATG 9 cut(s) 364, 413, 511, 535, 691, 752, 807, 1047, 1510
HincII GTYRAC 1 cut(s) 1101
HindII GTYRAC 1 cut(s) 1101
HindIII AAGCTT 1 cut(s) 1366
HinfI GANTC 3 cut(s) 538, 614, 1351
HpaII CCGG 1 cut(s) 1497
HphI GGTGA 4 cut(s) 326, 539, 1439, 1511
Hpy166II GTNNAC 2 cut(s) 1101, 1317
Hpy188I TCNGA 8 cut(s) 114, 178, 323, 382, 624, 700, 828, 1027
Hpy188III TCNNGA 8 cut(s) 11, 128, 332, 671, 722, 812, 1085, 1335
Hpy8I GTNNAC 2 cut(s) 1101, 1317
HpyAV CCTTC 4 cut(s) 61, 130, 487, 1399
HpyCH4III ACNGT 2 cut(s) 728, 901
HpyCH4IV ACGT 1 cut(s) 1206
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 865, 1374, 1457, 1469
HpyF3I CTNAG 5 cut(s) 111, 217, 697, 1107, 1113
HpySE526I ACGT 1 cut(s) 1206
Hsp92II CATG 9 cut(s) 364, 413, 511, 535, 691, 752, 807, 1047, 1510
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
LmnI GCTCC 3 cut(s) 16, 980, 1264
Lsp1109I GCAGC 3 cut(s) 843, 1250, 1472
LweI GCATC 6 cut(s) 475, 805, 1387, 1420, 1473, 1499
MaeI CTAG 2 cut(s) 1157, 1434
MaeII ACGT 1 cut(s) 1206
MaeIII GTNAC 6 cut(s) 527, 683, 1007, 1341, 1476, 1509
MalI GATC 6 cut(s) 9, 209, 1032, 1119, 1330, 1503
MboI GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
MboII GAAGA 6 cut(s) 130, 385, 566, 952, 1100, 1323
MflI RGATCY 2 cut(s) 1117, 1328
MhlI GDGCHC 2 cut(s) 112, 1174
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 3 cut(s) 164, 236, 581
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 532, 1345
MmeI TCCRAC 3 cut(s) 135, 815, 1281
Mox20I TGGCCA 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 608
MseI TTAA 8 cut(s) 249, 390, 462, 503, 563, 651, 1076, 1364
Msp20I TGGCCA 1 cut(s) 608
MspA1I CMGCKG 2 cut(s) 856, 1106
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 1 cut(s) 1497
MspR9I CCNGG 1 cut(s) 522
Mva1269I GAATGC 2 cut(s) 965, 1241
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 5 cut(s) 443, 865, 1374, 1457, 1469
NdeII GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
NlaIII CATG 9 cut(s) 364, 413, 511, 535, 691, 752, 807, 1047, 1510
NlaIV GGNNCC 2 cut(s) 307, 1305
NmeAIII GCCGAG 1 cut(s) 1498
NmuCI GTSAC 2 cut(s) 527, 683
NspI RCATGY 3 cut(s) 413, 807, 1047
PciI ACATGT 1 cut(s) 409
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 804
PctI GAATGC 2 cut(s) 965, 1241
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 1 cut(s) 614
PkrI GCNGC 5 cut(s) 76, 79, 858, 1240, 1462
PleI GAGTC 2 cut(s) 532, 1345
PpsI GAGTC 2 cut(s) 532, 1345
PscI ACATGT 1 cut(s) 409
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 520
PspEI GGTNACC 1 cut(s) 1007
PspFI CCCAGC 2 cut(s) 43, 147
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 307, 1305
PspPI GGNCC 3 cut(s) 306, 319, 1303
PsuI RGATCY 2 cut(s) 1117, 1328
PvuII CAGCTG 2 cut(s) 856, 1106
RsaI GTAC 6 cut(s) 195, 494, 603, 730, 992, 1162
RsaNI GTAC 6 cut(s) 194, 493, 602, 729, 991, 1161
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 8 cut(s) 249, 390, 462, 503, 563, 651, 1076, 1364
SatI GCNGC 5 cut(s) 75, 78, 857, 1239, 1461
Sau3AI GATC 6 cut(s) 7, 207, 1030, 1117, 1328, 1501
Sau96I GGNCC 3 cut(s) 306, 319, 1303
ScaI AGTACT 1 cut(s) 494
SchI GAGTC 2 cut(s) 532, 1345
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 2 cut(s) 112, 1174
SfaNI GCATC 6 cut(s) 475, 805, 1387, 1420, 1473, 1499
SfcI CTRYAG 1 cut(s) 103
SinI GGWCC 2 cut(s) 319, 1303
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 3 cut(s) 164, 236, 581
SsiI CCGC 3 cut(s) 74, 77, 818
SspMI CTAG 2 cut(s) 1157, 1434
SstI GAGCTC 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 2 cut(s) 1193, 1464
TaaI ACNGT 2 cut(s) 728, 901
TaiI ACGT 1 cut(s) 1209
TaqI TCGA 2 cut(s) 50, 798
TasI AATT 3 cut(s) 164, 236, 581
TatI WGTACW 2 cut(s) 492, 728
TauI GCSGC 2 cut(s) 77, 80
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 8 cut(s) 249, 390, 462, 503, 563, 651, 1076, 1364
Tru9I TTAA 8 cut(s) 249, 390, 462, 503, 563, 651, 1076, 1364
TscAI CASTG 1 cut(s) 750
TseFI GTSAC 2 cut(s) 527, 683
TseI GCWGC 3 cut(s) 856, 1238, 1460
Tsp45I GTSAC 2 cut(s) 527, 683
TspDTI ATGAA 9 cut(s) 79, 131, 377, 704, 765, 887, 1049, 1242, 1301
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 1 cut(s) 750
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 319, 1303
XapI RAATTY 2 cut(s) 236, 581
XceI RCATGY 3 cut(s) 413, 807, 1047
XmiI GTMKAC 1 cut(s) 1316
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 2 cut(s) 1157, 1434
ZrmI AGTACT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.