MD08G1075100.v1.1

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006231
Physical Location & Seq
Forward (+)
6081696 .. 6087686
5991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1075100.v1.1.491

Sequence Viewer

Length: 3321 bp
ATGAGGAGGCTCATACCTTGGCGCTTCAGAAGCGGAGTCTCCGACGACAGCAATCATCAGAAGCAGAAGCATCCAAAGGGATCCAAAGAAACCATGGAGAAGAACCAACAGACCAACAACAGTGCCGCCGACAGTGTGGAGTTTTCGAGCGAAAATGTTGCTGATTCAAGTAGTCACGGTCCAAATATCGTACTGAACCATGATTTCTCTGGAGGGCTGCATTCTTGGCACCCCAATCACTGCAACGCCTTTGTTGTGGATTCAGCAGGAAGTTATGCAGTTGTTACGAATCGCCAACAATGCTGGCAGGGATTGGAGCAAGATATTACTGAAAGAATTTCCCCTGGATTTACCTATTCGGTTTCAGCGTGTGTGGGAGTTTCAGGGCCTCTTCAGGGATCTGCTGAGGTCATGGCAACTTTGAAGCTGGAAAGCCGAGGCTCAGCAACTGGCTATGTGAAAATCGGAAGAAGTTCTGTATCAAATGGGAAATGGGAAAGTTTGGATGGAAAGTTTTCTCTGTCGACAATGCCTGATAGGGTGGTGTTTTATTTGGAAGGGCCTCCTGCTGGAGTCGACCTTCATATAAAATCAGTTATGATCTCTTGTTCTGAAGGCCAGTCTGAGAACCAAAACTTGGTGAATTCAAGTAGCAGAAATGCCACAAATATCATAGTGAACCATGATTTCTCTGGAGGACTGCATTCTTGGCACCCGAGTAACTGCAATGGCTTTGTAGTTTCCGCCGATTCTGGTCACCCAAAAGTAAAAGCTGGCAATTATGCTGTTGTTACAAATCGGAAGGAAAGTTGGCAAGGTTTGGAGCAAGATATCACACAGAGAATTTCCCCAGGCTCCACATATTTAGTTTCTGCATGTGTTGGAGTCTGCGGACCTCTTCAGGGCTCCACTGATGTCCTGGCAACTTTGAAGCTGGAATACCGAGGTTCAGCGACCAACTATTTGCAAGTTGGAAGATGTACTGTATCAAAGGGGAGGTGGGGAAATTTGGATGGGAAGTTCTCAGTGTCGACAAAACCTGATAGGGTTGTATTTTATTTGGAAGGGCCTTCGGTTGGAGTCGACCTTATTATAAAATCAGTTTTGATCTGTTCCTCGAGCCCAAGTGAATGCCAGAGTGGAAGGACGGGGAATTTCAATGATGGAGAAGAGAATATAATCCTAAACCCTAAATTTGAGGATGCCTTAAATAATTGGTCCGGAAGAGGTTGCAAAATTGTTTTGCATGACTCAATGGGAGATGGGAAAATCGTCCCTCAATCTGGAAAGGTTTTTGCAGCTGCAACAGAACGCACACAGAGCTGGAATGGCATCCAGCAGGACATCACTGGAAGAGTGCAGCGTAAACTAGCTTATGAGGCAACCACTGTGGTTAAAATATTTGGCAACAATGTCACTACTGCTGATGTCCGGGCTACCTTATGGGTGCAGTCACCAAATCAGCGTGAACAGTATATCGGCATTGCTAATGTGCAGGCAACAGACAAGGATTGGACACAGTTGCAGGGGAAGTTCCTTTTAAACGGCTCCCCGTCAAAAATAGTTGTTTATCTTGAAGGTCCACCGGCGGGAACAGATATTCTTGTCAATAGTTTTGTTGTCAAGCATGCTGAGAAAGCTCTTCCTTCACCTCCACCAGTCATTGAGTTGCCAGCATTTGGAGTAAATATAATTGAGAACAGCAATTTGAGTAATGGCACAAACGGGTGGTTTCCACTTGGTAATTGTACTCTGAGTGTCCGAACTGGTTCACCACATGTTCTCCCTCCAATGGCAATAGAGAGCCTTGGACCTCATGAACCTTTAAGTGGTTGCTACATTCTAGTAACCAAGCGCACGGAGACTTGGATGGGTCCTGCTCAGATGATTGGTGACAAATTGAAACTCTTTTTGACATATCAAGTGTCTGCTTGGGTTCGGATTGGTGCTGGAGCAACTGGTCCACAGAACGTCAACATTGCACTCGGCGTGGACAACCAGTGGGTAAACGGAGGGCAGGTTGAAGCAAGTGACACTAGATGGCATGAAATCGGTGGTTCCTTTAGAATTGAGAAGCAGCCATCAAAAGTAATGGTTTATATCCAAGGTCCAGCTGCAGGTGTAGACTTAATGGTTGCCGGACTTCAGATATTCCCTGTCGACAGACCAGCGAGATTTAGACATCTGAAGAGGCAGACTGATAAGATTCGTAAGTGCGATATTGTCCTGAAATTTTCAGGGTCGGACTCGAGCAGCATGCTTGGCAGCCTTGTGAAAGTTAAACAATCGCAAAACAGTTTTCCAATTGGGACATGTATAAGTAGAACAAACACTGACAATGAAGATTTCGTTGATTTCTTTGTTAAAAACTTCAACTGGGCTGTGTTTGGAAATGAATTGAAGTGGTATTGGACTGAACCACAACAAGGAAACTTCAACTACAAGGATGCTGATGATATGGTGGACTTGTGCAAGAATCACAACATAGAAATGCGAGGCCACTGTATTTTCTGGGAAGTCATCGACACAGTGCAGCAATGGATCCGTACATTGAGCCAGAGTGACTTGTCAACAGCTGTTCAAAATCGACTAACAGATCTTCTCACACGCTACAAAGGGAAGTTCAGGCACTATGATGTCAACAATGAGATGCTGCATGGCTCATTCTATCAGGATAGACTGGGAAAAGATATCAGAGCCAACATGTTCAAGACCGCAAACCTGTTGGATCCATCTGCAACTCTGTTTGTGAATGATTATCACATCGAGGATGGTTGCGATACTAGATCATCACCGGAAAAATACATTGATCAAATCCTTGATTTGCAGGAACAAGGTGCACCTGTTGGTGGCATTGGCATCCAAGGACATATTGATAGTCCAGTGGGTCCAATTGTTTGTTCTGCTCTTGACAAATTAGGCATTCTCGGCCTTCCAATCTGGTTCACAGAGCTTGATGTATCATCCACTAATGAATATGTTAGAGCAGACGATTTGGAAGTGATTCTTCGCGAAGCATTTGCCAATCCCGCAGTTGAAGGTGTAATGCTCTGGGGATTCTGGGAGCTGTTTATGAGTCGGGAAAATTCGCATTTAGTGAATGCAGAAGGGGATATCAACGAAGCAGGGAAACGTTACTTGGCTCTCAAGGAAGAGTGGCTGTCTGAAGCACACGGCCACATTGACGAGCAGGGAGAGTTCAGGTTTAGGGGCTTCCCAGGAGCATACAGTGTTGAAATTGTTACTGCCTCAAAGAAGCCTGTAAAAACATTCGTCGTCGACAAGGGCGAGTCCCCAGTGGAGGTCTCCATTGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

1107

Amino Acids

121.67

Weight (kDa)

5.97

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 62 - 189 1e-19 Carbohydrate binding domain
CBM_4_9 PF02018 223 - 357 6.8e-21 Carbohydrate binding domain
CBM_4_9 PF02018 392 - 530 1.4e-19 Carbohydrate binding domain
CBM_4_9 PF02018 563 - 705 6.9e-17 Carbohydrate binding domain
Glyco_hydro_10 PF00331 764 - 1020 9.4e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1094
AarI CACCTGC 1 cut(s) 2108
Acc36I ACCTGC 2 cut(s) 2008, 2108
AccB1I GGYRCC 2 cut(s) 228, 711
AccB7I CCANNNNNTGG 2 cut(s) 637, 1679
AccI GTMKAC 7 cut(s) 524, 576, 1031, 1083, 2124, 2160, 3279
AccII CGCG 1 cut(s) 3012
AccIII TCCGGA 1 cut(s) 1220
AciI CCGC 7 cut(s) 33, 126, 744, 891, 1589, 2715, 3030
AclI AACGTT 1 cut(s) 3133
AclWI GGATC 7 cut(s) 75, 88, 406, 2536, 2549, 2723, 2736
AcoI YGGCCR 1 cut(s) 3175
AcsI RAATTY 8 cut(s) 336, 643, 843, 1006, 1153, 1193, 2231, 3085
AcuI CTGAAG 7 cut(s) 10, 377, 633, 884, 2129, 2207, 3186
AfaI GTAC 4 cut(s) 192, 982, 1750, 2548
AflIII ACRYGT 3 cut(s) 1777, 2312, 2703
AjnI CCWGG 4 cut(s) 343, 850, 918, 3217
AjuI GAANNNNNNNTTGG 6 cut(s) 620, 652, 2884, 2916, 3122, 3154
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
Alw21I GWGCWC 1 cut(s) 2842
Alw26I GTCTC 3 cut(s) 43, 1856, 3310
Alw44I GTGCAC 1 cut(s) 2838
AlwI GGATC 7 cut(s) 75, 88, 406, 2536, 2549, 2723, 2736
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 3 cut(s) 715, 1117, 2248
Aor13HI TCCGGA 1 cut(s) 1220
AoxI GGCC 7 cut(s) 386, 560, 616, 1067, 2497, 2929, 3175
ApaLI GTGCAC 1 cut(s) 2838
ApoI RAATTY 8 cut(s) 336, 643, 843, 1006, 1153, 1193, 2231, 3085
ArsI GACNNNNNNTTYG 2 cut(s) 946, 978
Asp700I GAANNNNTTC 4 cut(s) 472, 514, 1768, 3003
AspLEI GCGC 2 cut(s) 24, 1857
AsuC2I CCSGG 1 cut(s) 1433
AsuHPI GGTGA 7 cut(s) 652, 749, 1446, 1641, 1764, 1904, 2784
AvaI CYCGRG 3 cut(s) 715, 1117, 2248
AvaII GGWCC 9 cut(s) 179, 893, 1218, 1580, 1811, 1874, 1961, 2108, 2888
BaeGI GKGCMC 1 cut(s) 2842
BamHI GGATCC 3 cut(s) 80, 2541, 2728
BanI GGYRCC 2 cut(s) 228, 711
BanII GRGCYC 2 cut(s) 908, 1124
BarI GAAGNNNNNNTAC 2 cut(s) 923, 955
Bbv12I GWGCWC 1 cut(s) 2842
BbvCI CCTCAGC 1 cut(s) 405
BccI CCATC 9 cut(s) 500, 1007, 1157, 1256, 1864, 2034, 2089, 2740, 2765
BceAI ACGGC 2 cut(s) 1561, 3190
BcgI CGANNNNNNTGC 6 cut(s) 140, 174, 2239, 2273, 3002, 3036
BciT130I CCWGG 4 cut(s) 345, 852, 920, 3219
BclI TGATCA 1 cut(s) 2809
BcnI CCSGG 1 cut(s) 1433
BcoDI GTCTC 3 cut(s) 43, 1856, 3310
BfaI CTAG 4 cut(s) 1370, 1844, 2037, 2784
BfmI CTRYAG 1 cut(s) 2115
BfoI RGCGCY 1 cut(s) 25
BfuAI ACCTGC 2 cut(s) 2008, 2108
BglII AGATCT 1 cut(s) 2596
BlpI GCTNAGC 1 cut(s) 442
Bme1390I CCNGG 5 cut(s) 345, 852, 920, 1433, 3219
Bme18I GGWCC 9 cut(s) 179, 893, 1218, 1580, 1811, 1874, 1961, 2108, 2888
BmeT110I CYCGRG 3 cut(s) 715, 1117, 2248
BmrFI CCNGG 5 cut(s) 345, 852, 920, 1433, 3219
BmrI ACTGGG 3 cut(s) 2386, 2690, 3290
BmsI GCATC 6 cut(s) 79, 1192, 1341, 2437, 2640, 2868
BmuI ACTGGG 3 cut(s) 2386, 2690, 3290
BpmI CTGGAG 4 cut(s) 231, 591, 714, 1971
Bpu10I CCTNAGC 1 cut(s) 405
Bpu1102I GCTNAGC 1 cut(s) 442
BpuEI CTTGAG 1 cut(s) 3131
BpuMI CCSGG 1 cut(s) 1433
BsaI GGTCTC 1 cut(s) 3310
BsaWI WCCGGW 2 cut(s) 1220, 2794
BsaXI ACNNNNNCTCC 4 cut(s) 1767, 1797, 2004, 2034
Bse118I RCCGGY 1 cut(s) 1585
Bse3DI GCAATG 5 cut(s) 733, 1482, 1977, 2543, 3309
BseAI TCCGGA 1 cut(s) 1220
BseBI CCWGG 4 cut(s) 345, 852, 920, 3219
BseMI GCAATG 5 cut(s) 733, 1482, 1977, 2543, 3309
BseMII CTCAG 7 cut(s) 396, 456, 615, 1038, 1623, 1745, 1895
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 2842
BsgI GTGCAG 4 cut(s) 1379, 1469, 1514, 2552
Bsh1236I CGCG 1 cut(s) 3012
BshFI GGCC 7 cut(s) 388, 562, 618, 1069, 2499, 2931, 3177
BshNI GGYRCC 2 cut(s) 228, 711
BsiHKAI GWGCWC 1 cut(s) 2842
BsiHKCI CYCGRG 3 cut(s) 715, 1117, 2248
BsiSI CCGG 5 cut(s) 1221, 1432, 1586, 2139, 2795
BslFI GGGAC 3 cut(s) 1259, 2323, 3277
BsmAI GTCTC 3 cut(s) 43, 1856, 3310
BsmFI GGGAC 3 cut(s) 1259, 2323, 3277
BsmI GAATGC 5 cut(s) 220, 703, 1136, 2922, 3106
BsnI GGCC 7 cut(s) 388, 562, 618, 1069, 2499, 2931, 3177
Bso31I GGTCTC 1 cut(s) 3310
BsoBI CYCGRG 3 cut(s) 715, 1117, 2248
Bsp1286I GDGCHC 3 cut(s) 908, 1124, 2842
Bsp13I TCCGGA 1 cut(s) 1220
Bsp143I GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
Bsp1720I GCTNAGC 1 cut(s) 442
Bsp19I CCATGG 1 cut(s) 93
Bsp68I TCGCGA 1 cut(s) 3012
BspACI CCGC 7 cut(s) 33, 126, 744, 891, 1589, 2715, 3030
BspANI GGCC 7 cut(s) 388, 562, 618, 1069, 2499, 2931, 3177
BspCNI CTCAG 7 cut(s) 397, 455, 616, 1037, 1624, 1746, 1894
BspEI TCCGGA 1 cut(s) 1220
BspFNI CGCG 1 cut(s) 3012
BspHI TCATGA 1 cut(s) 1816
BspMAI CTGCAG 1 cut(s) 2119
BspMI ACCTGC 2 cut(s) 2008, 2108
BspPI GGATC 7 cut(s) 75, 88, 406, 2536, 2549, 2723, 2736
BspQI GCTCTTC 1 cut(s) 1647
BspT107I GGYRCC 2 cut(s) 228, 711
BspTNI GGTCTC 1 cut(s) 3310
BsrDI GCAATG 5 cut(s) 733, 1482, 1977, 2543, 3309
BsrFI RCCGGY 1 cut(s) 1585
BssAI RCCGGY 1 cut(s) 1585
BssMI GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
BssT1I CCWWGG 5 cut(s) 17, 93, 1807, 2104, 2863
Bst2UI CCWGG 4 cut(s) 345, 852, 920, 3219
Bst6I CTCTTC 8 cut(s) 396, 903, 1164, 1219, 1348, 1647, 2183, 3147
BstC8I GCNNGC 6 cut(s) 305, 775, 1497, 1629, 1674, 2258
BstDEI CTNAG 7 cut(s) 405, 442, 624, 1024, 1632, 1754, 1881
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 755
BstFNI CGCG 1 cut(s) 3012
BstH2I RGCGCY 1 cut(s) 25
BstHHI GCGC 2 cut(s) 24, 1857
BstKTI GATC 9 cut(s) 83, 401, 603, 1110, 2544, 2599, 2731, 2789, 2812
BstMAI GTCTC 3 cut(s) 43, 1856, 3310
BstMBI GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
BstNI CCWGG 4 cut(s) 345, 852, 920, 3219
BstNSI RCATGY 6 cut(s) 879, 1631, 1781, 2260, 2316, 2707
BstPI GGTNACC 1 cut(s) 755
BstSCI CCNGG 5 cut(s) 343, 850, 918, 1431, 3217
BstSFI CTRYAG 1 cut(s) 2115
BstSLI GKGCMC 1 cut(s) 2842
BstUI CGCG 1 cut(s) 3012
BstX2I RGATCY 5 cut(s) 80, 398, 2541, 2596, 2728
BstYI RGATCY 5 cut(s) 80, 398, 2541, 2596, 2728
BsuRI GGCC 7 cut(s) 388, 562, 618, 1069, 2499, 2931, 3177
BtgI CCRYGG 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 238
BtuMI TCGCGA 1 cut(s) 3012
BveI ACCTGC 2 cut(s) 2008, 2108
Cac8I GCNNGC 6 cut(s) 305, 775, 1497, 1629, 1674, 2258
CaiI CAGNNNCTG 1 cut(s) 449
CciI TCATGA 1 cut(s) 1816
CfoI GCGC 2 cut(s) 24, 1857
Cfr10I RCCGGY 1 cut(s) 1585
Csp6I GTAC 4 cut(s) 191, 981, 1749, 2547
CspCI CAANNNNNGTGG 2 cut(s) 1371, 1406
CviQI GTAC 4 cut(s) 191, 981, 1749, 2547
DdeI CTNAG 7 cut(s) 405, 442, 624, 1024, 1632, 1754, 1881
DpnI GATC 9 cut(s) 82, 400, 602, 1109, 2543, 2598, 2730, 2788, 2811
DpnII GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
DraI TTTAAA 1 cut(s) 1542
EaeI YGGCCR 1 cut(s) 3175
Eam1104I CTCTTC 8 cut(s) 396, 903, 1164, 1219, 1348, 1647, 2183, 3147
EarI CTCTTC 8 cut(s) 396, 903, 1164, 1219, 1348, 1647, 2183, 3147
EciI GGCGGA 1 cut(s) 733
Eco130I CCWWGG 5 cut(s) 17, 93, 1807, 2104, 2863
Eco24I GRGCYC 2 cut(s) 908, 1124
Eco31I GGTCTC 1 cut(s) 3310
Eco32I GATATC 3 cut(s) 832, 2692, 3115
Eco47I GGWCC 9 cut(s) 179, 893, 1218, 1580, 1811, 1874, 1961, 2108, 2888
Eco57I CTGAAG 7 cut(s) 10, 377, 633, 884, 2129, 2207, 3186
Eco88I CYCGRG 3 cut(s) 715, 1117, 2248
Eco91I GGTNACC 1 cut(s) 755
EcoO109I RGGNCCY 4 cut(s) 386, 560, 1067, 1874
EcoO65I GGTNACC 1 cut(s) 755
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 4 cut(s) 343, 850, 918, 3217
EcoRV GATATC 3 cut(s) 832, 2692, 3115
EcoT14I CCWWGG 5 cut(s) 17, 93, 1807, 2104, 2863
EcoT38I GRGCYC 2 cut(s) 908, 1124
ErhI CCWWGG 5 cut(s) 17, 93, 1807, 2104, 2863
FalI AAGNNNNNCTT 2 cut(s) 2991, 3023
FaqI GGGAC 3 cut(s) 1259, 2323, 3277
FauI CCCGC 2 cut(s) 1582, 3037
FbaI TGATCA 1 cut(s) 2809
FblI GTMKAC 7 cut(s) 524, 576, 1031, 1083, 2124, 2160, 3279
FriOI GRGCYC 2 cut(s) 908, 1124
FspBI CTAG 4 cut(s) 1370, 1844, 2037, 2784
GlaI GCGC 2 cut(s) 23, 1856
GsuI CTGGAG 4 cut(s) 231, 591, 714, 1971
HaeII RGCGCY 1 cut(s) 25
HaeIII GGCC 7 cut(s) 388, 562, 618, 1069, 2499, 2931, 3177
HapII CCGG 5 cut(s) 1221, 1432, 1586, 2139, 2795
HhaI GCGC 2 cut(s) 24, 1857
Hin6I GCGC 2 cut(s) 22, 1855
HinP1I GCGC 2 cut(s) 22, 1855
HincII GTYRAC 9 cut(s) 525, 577, 1032, 1084, 1975, 2161, 2571, 2641, 3280
HindII GTYRAC 9 cut(s) 525, 577, 1032, 1084, 1975, 2161, 2571, 2641, 3280
HpaII CCGG 5 cut(s) 1221, 1432, 1586, 2139, 2795
HphI GGTGA 7 cut(s) 652, 749, 1446, 1641, 1764, 1904, 2784
Hpy99I CGWCG 3 cut(s) 47, 3278, 3281
HpyCH4IV ACGT 2 cut(s) 1971, 3133
HpyF3I CTNAG 7 cut(s) 405, 442, 624, 1024, 1632, 1754, 1881
HpySE526I ACGT 2 cut(s) 1971, 3133
HspAI GCGC 2 cut(s) 22, 1855
Kpn2I TCCGGA 1 cut(s) 1220
Ksp22I TGATCA 1 cut(s) 2809
Kzo9I GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
LguI GCTCTTC 1 cut(s) 1647
LmnI GCTCC 8 cut(s) 316, 823, 860, 911, 1553, 1952, 3064, 3221
LweI GCATC 6 cut(s) 79, 1192, 1341, 2437, 2640, 2868
MaeI CTAG 4 cut(s) 1370, 1844, 2037, 2784
MaeII ACGT 2 cut(s) 1971, 3133
MalI GATC 9 cut(s) 82, 400, 602, 1109, 2543, 2598, 2730, 2788, 2811
MboI GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
MfeI CAATTG 2 cut(s) 2304, 2892
MflI RGATCY 5 cut(s) 80, 398, 2541, 2596, 2728
MhlI GDGCHC 3 cut(s) 908, 1124, 2842
MlyI GAGTC 8 cut(s) 45, 582, 894, 1089, 1244, 2240, 3085, 3299
MmeI TCCRAC 6 cut(s) 66, 862, 952, 1057, 2223, 2706
MroI TCCGGA 1 cut(s) 1220
MroXI GAANNNNTTC 4 cut(s) 472, 514, 1768, 3003
MseI TTAA 7 cut(s) 1208, 1395, 1541, 1826, 2129, 2280, 2364
MslI CAYNNNNRTG 2 cut(s) 2489, 2634
MspA1I CMGCKG 3 cut(s) 1301, 2114, 2576
MspI CCGG 5 cut(s) 1221, 1432, 1586, 2139, 2795
MspR9I CCNGG 5 cut(s) 345, 852, 920, 1433, 3219
MunI CAATTG 2 cut(s) 2304, 2892
Mva1269I GAATGC 5 cut(s) 220, 703, 1136, 2922, 3106
MvaI CCWGG 4 cut(s) 345, 852, 920, 3219
MvnI CGCG 1 cut(s) 3012
NciI CCSGG 1 cut(s) 1433
NcoI CCATGG 1 cut(s) 93
NdeII GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
NmeAIII GCCGAG 3 cut(s) 461, 1965, 2907
NmuCI GTSAC 7 cut(s) 173, 755, 1414, 1452, 1892, 2030, 2561
NruI TCGCGA 1 cut(s) 3012
NspI RCATGY 6 cut(s) 879, 1631, 1781, 2260, 2316, 2707
PaeI GCATGC 2 cut(s) 1631, 2260
PaeR7I CTCGAG 2 cut(s) 1117, 2248
PagI TCATGA 1 cut(s) 1816
PaqCI CACCTGC 1 cut(s) 2108
PciI ACATGT 3 cut(s) 1777, 2312, 2703
PciSI GCTCTTC 1 cut(s) 1647
PcsI WCGNNNNNNNCGW 2 cut(s) 365, 3285
PctI GAATGC 5 cut(s) 220, 703, 1136, 2922, 3106
PdmI GAANNNNTTC 4 cut(s) 472, 514, 1768, 3003
PfeI GAWTC 8 cut(s) 164, 260, 289, 749, 2206, 2476, 3004, 3057
PflMI CCANNNNNTGG 2 cut(s) 637, 1679
PleI GAGTC 8 cut(s) 44, 581, 893, 1088, 1244, 2240, 3084, 3298
PpsI GAGTC 8 cut(s) 44, 581, 893, 1088, 1244, 2240, 3084, 3298
PpuMI RGGWCCY 1 cut(s) 1874
PscI ACATGT 3 cut(s) 1777, 2312, 2703
PsiI TTATAA 1 cut(s) 1094
Psp1406I AACGTT 1 cut(s) 3133
Psp5II RGGWCCY 1 cut(s) 1874
Psp6I CCWGG 4 cut(s) 343, 850, 918, 3217
PspEI GGTNACC 1 cut(s) 755
PspGI CCWGG 4 cut(s) 343, 850, 918, 3217
PspPPI RGGWCCY 1 cut(s) 1874
PspXI VCTCGAGB 2 cut(s) 1117, 2248
PstI CTGCAG 1 cut(s) 2119
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 5 cut(s) 80, 398, 2541, 2596, 2728
PvuII CAGCTG 3 cut(s) 1301, 2114, 2576
RruI TCGCGA 1 cut(s) 3012
RsaI GTAC 4 cut(s) 192, 982, 1750, 2548
RsaNI GTAC 4 cut(s) 191, 981, 1749, 2547
RseI CAYNNNNRTG 2 cut(s) 2489, 2634
SalI GTCGAC 6 cut(s) 523, 575, 1030, 1082, 2159, 3278
SapI GCTCTTC 1 cut(s) 1647
SaqAI TTAA 7 cut(s) 1208, 1395, 1541, 1826, 2129, 2280, 2364
Sau3AI GATC 9 cut(s) 80, 398, 600, 1107, 2541, 2596, 2728, 2786, 2809
SchI GAGTC 8 cut(s) 45, 582, 894, 1089, 1244, 2240, 3085, 3299
ScrFI CCNGG 5 cut(s) 345, 852, 920, 1433, 3219
SduI GDGCHC 3 cut(s) 908, 1124, 2842
SfaNI GCATC 6 cut(s) 79, 1192, 1341, 2437, 2640, 2868
SfcI CTRYAG 1 cut(s) 2115
Sfr274I CTCGAG 2 cut(s) 1117, 2248
SgrAI CRCCGGYG 1 cut(s) 1585
SinI GGWCC 9 cut(s) 179, 893, 1218, 1580, 1811, 1874, 1961, 2108, 2888
SlaI CTCGAG 2 cut(s) 1117, 2248
SmiMI CAYNNNNRTG 2 cut(s) 2489, 2634
SmlI CTYRAG 3 cut(s) 1117, 2248, 3146
SmoI CTYRAG 3 cut(s) 1117, 2248, 3146
SphI GCATGC 2 cut(s) 1631, 2260
SsiI CCGC 7 cut(s) 33, 126, 744, 891, 1589, 2715, 3030
SspI AATATT 1 cut(s) 1401
SspMI CTAG 4 cut(s) 1370, 1844, 2037, 2784
StyD4I CCNGG 5 cut(s) 343, 850, 918, 1431, 3217
StyI CCWWGG 5 cut(s) 17, 93, 1807, 2104, 2863
TaiI ACGT 2 cut(s) 1974, 3136
TatI WGTACW 2 cut(s) 980, 1748
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 8 cut(s) 164, 260, 289, 749, 2206, 2476, 3004, 3057
Tru1I TTAA 7 cut(s) 1208, 1395, 1541, 1826, 2129, 2280, 2364
Tru9I TTAA 7 cut(s) 1208, 1395, 1541, 1826, 2129, 2280, 2364
TseFI GTSAC 7 cut(s) 173, 755, 1414, 1452, 1892, 2030, 2561
Tsp45I GTSAC 7 cut(s) 173, 755, 1414, 1452, 1892, 2030, 2561
TspDTI ATGAA 6 cut(s) 572, 1833, 2061, 2355, 2409, 2988
TspGWI ACGGA 3 cut(s) 1874, 2025, 2534
Van91I CCANNNNNTGG 2 cut(s) 637, 1679
VneI GTGCAC 1 cut(s) 2838
VpaK11BI GGWCC 9 cut(s) 179, 893, 1218, 1580, 1811, 1874, 1961, 2108, 2888
XapI RAATTY 8 cut(s) 336, 643, 843, 1006, 1153, 1193, 2231, 3085
XceI RCATGY 6 cut(s) 879, 1631, 1781, 2260, 2316, 2707
XcmI CCANNNNNNNNNTGG 4 cut(s) 91, 206, 689, 916
XhoI CTCGAG 2 cut(s) 1117, 2248
XmiI GTMKAC 7 cut(s) 524, 576, 1031, 1083, 2124, 2160, 3279
XmnI GAANNNNTTC 4 cut(s) 472, 514, 1768, 3003
XspI CTAG 4 cut(s) 1370, 1844, 2037, 2784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.