Rorug06G0379000

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006231
Physical Location & Seq
Forward (+)
52496962 .. 52498286
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0379000.1

Sequence Viewer

Length: 900 bp
ATGAGTCGGCGTTGGACTGCGAGTTATACCCTGCTCCCAAAGGCCAAAAAGGGAAAACAAAGCCCAGACGATTTGTCGGAGGACCAGCTCCGTCAGATCTACACATTGGTTCCGGACGCGGGTTTCCTAATAGGAAAAAATGAGAGGGATCAGACCAAAATGGAAGAGGATAAGTATGATGAAGACAGCGTCAAAGCAATTTTTTCTGAGCTGCCAGGTTATGAAGACACGAGTATGGTACCAAGATCATCTTCAGATTTCTCAGAGCACCAGCTCCGTCAGCTCTTCGACTTGGATCCGGCACCTGGTCCTGATGACATGTTAGAAGATAAGGAACCTATAAAATGGTTTGAGGTGGTTTGTAAGGTGTGTGGTTATGTTGTGAGTGGCCTCCAGGAGGGTTCTAGCTTGCAGGATTGTCGTGCCACCCATGTACATGCATCATGCCACCGCGATTTTTTGCTTAAACTTCCTACAGGTATTAATCAATGTCATGAAGACACCATGAGTATGGCACCAAGACCATCATATCACTCAGAGGCGGAGCTCCTTCGGATCTACTTGGTGGATTCATACACTACTGGACATAGGAGGTCTGCTGCGCCTCCTAGTGAGGAGCCTGTAGTGGTTTGGTATGCAGTGATTTGTAAGGTCTGTAAGAAGGATGTATATACCACCAAGGATTATCCTAGCTTGCAGGATAATCCTTGGTTGCAAAAACATGCTTTGATGCACAGTGAGTACTTGTCAATGGATGGCCCTACAGTACCAGATATAGATCTTGAAGCTTACGAGAACAAGCTGCTGAAAGGGTATGATGGAGGCTTTAGCTTGGATAAAGATGATGCATCTCTGCATGCAGGAGGGATTTGTAAAGATTTGTTGGAGGTGTCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

299

Amino Acids

33.78

Weight (kDa)

4.93

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 238
AccB1I GGYRCC 3 cut(s) 238, 301, 514
AccII CGCG 2 cut(s) 119, 453
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 3 cut(s) 119, 451, 542
AclWI GGATC 4 cut(s) 156, 290, 303, 563
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 4 cut(s) 240, 435, 743, 768
AfiI CCNNNNNNNGG 3 cut(s) 119, 305, 397
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 1 cut(s) 785
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 3 cut(s) 214, 304, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
Alw21I GWGCWC 2 cut(s) 270, 549
AlwI GGATC 4 cut(s) 156, 290, 303, 563
Aor13HI TCCGGA 1 cut(s) 112
AoxI GGCC 3 cut(s) 42, 388, 757
ApeKI GCWGC 3 cut(s) 211, 599, 802
AseI ATTAAT 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 82, 308, 758
AvaII GGWCC 2 cut(s) 82, 308
BamHI GGATCC 1 cut(s) 295
BanI GGYRCC 3 cut(s) 238, 301, 514
BanII GRGCYC 1 cut(s) 549
BauI CACGAG 1 cut(s) 229
BbsI GAAGAC 3 cut(s) 189, 231, 504
Bbv12I GWGCWC 2 cut(s) 270, 549
BbvI GCAGC 3 cut(s) 198, 586, 789
BccI CCATC 3 cut(s) 532, 749, 812
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 3 cut(s) 216, 306, 395
BfaI CTAG 3 cut(s) 405, 609, 690
BfmI CTRYAG 3 cut(s) 474, 621, 762
BglII AGATCT 2 cut(s) 96, 778
BisI GCNGC 3 cut(s) 212, 600, 803
BlsI GCNGC 3 cut(s) 213, 601, 804
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 216, 306, 395
Bme18I GGWCC 2 cut(s) 82, 308
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 3 cut(s) 82, 308, 758
BmiI GGNNCC 7 cut(s) 111, 240, 297, 303, 336, 516, 618
BmrFI CCNGG 3 cut(s) 216, 306, 395
BmsI GCATC 4 cut(s) 449, 720, 835, 857
BpiI GAAGAC 3 cut(s) 189, 231, 504
BpmI CTGGAG 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 2 cut(s) 678, 707
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 305, 397
Bse1I ACTGG 1 cut(s) 586
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 3 cut(s) 216, 306, 395
BseDI CCNNGG 2 cut(s) 678, 707
BseGI GGATG 2 cut(s) 670, 760
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 3 cut(s) 119, 305, 397
BseMII CTCAG 3 cut(s) 198, 276, 549
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 1 cut(s) 629
BseXI GCAGC 3 cut(s) 198, 586, 789
Bsh1236I CGCG 2 cut(s) 119, 453
BshFI GGCC 3 cut(s) 44, 390, 759
BshNI GGYRCC 3 cut(s) 238, 301, 514
BsiHKAI GWGCWC 2 cut(s) 270, 549
BsiSI CCGG 2 cut(s) 113, 299
BslI CCNNNNNNNGG 3 cut(s) 119, 305, 397
BsnI GGCC 3 cut(s) 44, 390, 759
Bsp1286I GDGCHC 2 cut(s) 270, 549
Bsp13I TCCGGA 1 cut(s) 112
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 6 cut(s) 96, 148, 245, 295, 555, 778
BspACI CCGC 3 cut(s) 119, 451, 542
BspANI GGCC 3 cut(s) 44, 390, 759
BspCNI CTCAG 3 cut(s) 199, 275, 548
BspEI TCCGGA 1 cut(s) 112
BspFNI CGCG 2 cut(s) 119, 453
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 7 cut(s) 111, 240, 297, 303, 336, 516, 618
BspPI GGATC 4 cut(s) 156, 290, 303, 563
BspQI GCTCTTC 1 cut(s) 290
BspT107I GGYRCC 3 cut(s) 238, 301, 514
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 1 cut(s) 586
BssECI CCNNGG 2 cut(s) 678, 707
BssMI GATC 6 cut(s) 96, 148, 245, 295, 555, 778
BssSI CACGAG 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 678, 707
Bst2BI CACGAG 1 cut(s) 229
Bst2UI CCWGG 3 cut(s) 216, 306, 395
Bst4CI ACNGT 2 cut(s) 737, 766
Bst6I CTCTTC 2 cut(s) 159, 290
BstAUI TGTACA 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 410, 695, 858
BstDEI CTNAG 3 cut(s) 207, 262, 535
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 2 cut(s) 670, 760
BstFNI CGCG 2 cut(s) 119, 453
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 6 cut(s) 99, 151, 248, 298, 558, 781
BstMBI GATC 6 cut(s) 96, 148, 245, 295, 555, 778
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 3 cut(s) 216, 306, 395
BstNSI RCATGY 4 cut(s) 322, 440, 725, 860
BstSCI CCNGG 3 cut(s) 214, 304, 393
BstSFI CTRYAG 3 cut(s) 474, 621, 762
BstUI CGCG 2 cut(s) 119, 453
BstV1I GCAGC 3 cut(s) 198, 586, 789
BstV2I GAAGAC 3 cut(s) 189, 231, 504
BstX2I RGATCY 4 cut(s) 96, 295, 555, 778
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 4 cut(s) 96, 295, 555, 778
BsuRI GGCC 3 cut(s) 44, 390, 759
BtsCI GGATG 2 cut(s) 670, 760
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 645, 742
Cac8I GCNNGC 3 cut(s) 410, 695, 858
CciI TCATGA 1 cut(s) 493
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 82, 308, 758
CseI GACGC 2 cut(s) 125, 178
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 4 cut(s) 239, 434, 742, 767
CviAII CATG 8 cut(s) 319, 431, 437, 444, 494, 505, 722, 857
CviQI GTAC 4 cut(s) 239, 434, 742, 767
DdeI CTNAG 3 cut(s) 207, 262, 535
DpnI GATC 6 cut(s) 98, 150, 247, 297, 557, 780
DpnII GATC 6 cut(s) 96, 148, 245, 295, 555, 778
DriI GACNNNNNGTC 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 159, 290
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 2 cut(s) 159, 290
EciI GGCGGA 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 678, 707
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 82, 308
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRII CCWGG 3 cut(s) 214, 304, 393
EcoT14I CCWWGG 2 cut(s) 678, 707
EcoT22I ATGCAT 2 cut(s) 442, 850
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 2 cut(s) 678, 707
FaeI CATG 8 cut(s) 322, 434, 440, 447, 497, 508, 725, 860
FalI AAGNNNNNCTT 2 cut(s) 235, 267
FatI CATG 8 cut(s) 318, 430, 436, 443, 493, 504, 721, 856
FauI CCCGC 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 212, 600, 803
FokI GGATG 2 cut(s) 677, 767
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 3 cut(s) 212, 600, 803
FspBI CTAG 3 cut(s) 405, 609, 690
GlaI GCGC 1 cut(s) 603
GluI GCNGC 3 cut(s) 212, 600, 803
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 3 cut(s) 44, 390, 759
HapII CCGG 2 cut(s) 113, 299
HgaI GACGC 2 cut(s) 125, 178
HhaI GCGC 1 cut(s) 604
Hin1II CATG 8 cut(s) 322, 434, 440, 447, 497, 508, 725, 860
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HindIII AAGCTT 1 cut(s) 786
HinfI GANTC 2 cut(s) 4, 569
HpaII CCGG 2 cut(s) 113, 299
Hpy188I TCNGA 9 cut(s) 79, 96, 153, 208, 256, 265, 538, 555, 899
Hpy188III TCNNGA 4 cut(s) 113, 311, 494, 782
HpyAV CCTTC 2 cut(s) 560, 655
HpyCH4III ACNGT 2 cut(s) 737, 766
HpyCH4V TGCA 9 cut(s) 412, 440, 638, 697, 715, 733, 848, 856, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 3 cut(s) 207, 262, 535
Hsp92II CATG 8 cut(s) 322, 434, 440, 447, 497, 508, 725, 860
HspAI GCGC 1 cut(s) 602
Kpn2I TCCGGA 1 cut(s) 112
KpnI GGTACC 1 cut(s) 242
Kzo9I GATC 6 cut(s) 96, 148, 245, 295, 555, 778
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 6 cut(s) 39, 93, 279, 544, 552, 616
Lsp1109I GCAGC 3 cut(s) 198, 586, 789
LweI GCATC 4 cut(s) 449, 720, 835, 857
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 3 cut(s) 405, 609, 690
MalI GATC 6 cut(s) 98, 150, 247, 297, 557, 780
MboI GATC 6 cut(s) 96, 148, 245, 295, 555, 778
MboII GAAGA 7 cut(s) 176, 194, 236, 243, 277, 338, 509
MflI RGATCY 4 cut(s) 96, 295, 555, 778
MhlI GDGCHC 2 cut(s) 270, 549
MluCI AATT 1 cut(s) 198
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 2 cut(s) 57, 864
Mph1103I ATGCAT 2 cut(s) 442, 850
MroI TCCGGA 1 cut(s) 112
MseI TTAA 2 cut(s) 465, 483
MslI CAYNNNNRTG 3 cut(s) 233, 435, 509
MspI CCGG 2 cut(s) 113, 299
MspR9I CCNGG 3 cut(s) 216, 306, 395
MvaI CCWGG 3 cut(s) 216, 306, 395
MvnI CGCG 2 cut(s) 119, 453
MwoI GCNNNNNNNGC 1 cut(s) 280
NdeII GATC 6 cut(s) 96, 148, 245, 295, 555, 778
NlaIII CATG 8 cut(s) 322, 434, 440, 447, 497, 508, 725, 860
NlaIV GGNNCC 7 cut(s) 111, 240, 297, 303, 336, 516, 618
NsiI ATGCAT 2 cut(s) 442, 850
NspI RCATGY 4 cut(s) 322, 440, 725, 860
PaeI GCATGC 1 cut(s) 860
PagI TCATGA 1 cut(s) 493
PciI ACATGT 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 569
PflFI GACNNNGTC 1 cut(s) 188
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 3 cut(s) 213, 601, 804
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PscI ACATGT 1 cut(s) 318
PshBI ATTAAT 1 cut(s) 483
Psp124BI GAGCTC 1 cut(s) 549
Psp6I CCWGG 3 cut(s) 214, 304, 393
PspGI CCWGG 3 cut(s) 214, 304, 393
PspN4I GGNNCC 7 cut(s) 111, 240, 297, 303, 336, 516, 618
PspPI GGNCC 3 cut(s) 82, 308, 758
PsuI RGATCY 4 cut(s) 96, 295, 555, 778
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 4 cut(s) 240, 435, 743, 768
RsaNI GTAC 4 cut(s) 239, 434, 742, 767
RseI CAYNNNNRTG 3 cut(s) 233, 435, 509
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 290
SaqAI TTAA 2 cut(s) 465, 483
SatI GCNGC 3 cut(s) 212, 600, 803
Sau3AI GATC 6 cut(s) 96, 148, 245, 295, 555, 778
Sau96I GGNCC 3 cut(s) 82, 308, 758
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 3 cut(s) 216, 306, 395
SduI GDGCHC 2 cut(s) 270, 549
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 4 cut(s) 449, 720, 835, 857
SfcI CTRYAG 3 cut(s) 474, 621, 762
SinI GGWCC 2 cut(s) 82, 308
SmiMI CAYNNNNRTG 3 cut(s) 233, 435, 509
SphI GCATGC 1 cut(s) 860
Sse9I AATT 1 cut(s) 198
SsiI CCGC 3 cut(s) 119, 451, 542
SspMI CTAG 3 cut(s) 405, 609, 690
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 214, 304, 393
StyI CCWWGG 2 cut(s) 678, 707
TaaI ACNGT 2 cut(s) 737, 766
TaqI TCGA 1 cut(s) 288
TasI AATT 1 cut(s) 198
TatI WGTACW 2 cut(s) 433, 741
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 465, 483
Tru9I TTAA 2 cut(s) 465, 483
TscAI CASTG 2 cut(s) 645, 742
TseI GCWGC 3 cut(s) 211, 599, 802
TspDTI ATGAA 4 cut(s) 195, 237, 510, 561
TspGWI ACGGA 2 cut(s) 80, 266
TspRI CASTG 2 cut(s) 645, 742
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 82, 308
VspI ATTAAT 1 cut(s) 483
XagI CCTNNNNNAGG 1 cut(s) 395
XceI RCATGY 4 cut(s) 322, 440, 725, 860
XspI CTAG 3 cut(s) 405, 609, 690
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 2 cut(s) 442, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.