Rh6BG502800

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006231
Physical Location & Seq
Reverse (-)
70178678 .. 70183246
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG502800.1

Sequence Viewer

Length: 2682 bp
ATGGTTAATTCAAGTAGTAGTCATGGTACCAATATCATAGTGAACCATGATTTCTGTGGAGGGCTGCACTCTTGGCACCCCAATTGCTGTGAGGGATATGTAGTTTCAGCTCATTCTGGCCACCCACAAGCAAATTCGGGTGGCAATTATGCAGTTGTTACTAATCGGAAAGAATGCTGGCAAGGATTGGAGCAGGATATCACTGGGAGGGTGTCCCCTGGTTTGACTTACTTGGTTGCGGCTTCTGTTGGGGTTTCGGGACCTCTCGTGGGATGTGTTGATGTCCTGGCAACATTGAAAATGGAATGCCAGGGTTCAGAAACCAAGTATTCCTTAGTTGGGAGAAGTTCTGTATCAAATGGGAAATGGGAAAAGTTGGAAGGCAAATTTTCACTGTCAACAATGCCTGATAAGGTTGTGTTTTACTTGGAAGGGCCTTCTCCTGGAATTGACTTGCTTATACAATCTGTAGTGATCTCTTGTTCCAATCCAAAGGGACAGCGGCACGGAAATGCCAGTGATGGAGGTGACGATATAGTCCTAAACCCAAAATTTGAGGATGGCTTGACTAATTGGACTGGAAGAGGCTGCAAGGTAGTTCTACACGACTCGATGGGAGATGGGAAAATCGTCCCTCAATCTGGAAAAGTTTTTGCTGCTGCAACAGAACGCACACAGAGCTGGAATGGCATTCAGCAGGATATTACTGGAAGAGTGCAACGAAAGCTAGCTTACGAGGCTACTGCTGTTGTCCGGATATTTGGTAACAATGTCACGAGTTCTGATGTACGGGCTACCTTATGGGTGCAGTCACCAAATGGTCGTGAACAGTATATTGGCATTTCTAAGGTACAGGCAACAGACAAGGATTGGGCACAGTTGAAGGGGAAATTCCTTTTAAACGGATCCCCATCCAAAGTAGTCGTTTATTTAGAAGGTCCACCAGCAGGAACTGATATTCTTGTCAACAGTTTCGTTGTCAAGCACGCACAGAAAGTCCCTCCTTCTTCTCCACCAGATATTGAGAATCCTGCTTTTGGAGTAAATATAATTGAGAACAGTAATCTAAGTAATGGCACCAATGGGTGGTTTCCTCTTGGTAATTGCACTTTGAGTGTGGGAACTGGTTCACCGCATATACTTCCTCCAATGGCGAGAGATAGCCTTGGCGCTCATGAACCTTTAAGTGGTCGCTTCATCCTTGTAACCAAGCGCACACAGACTTGGATGGGTCCTGCTCAGATGATTGGTGACAAATTGAAACTCTTTTTGACATATCAAGTATCTGCTTGGGTTCGGATTGGCGCTGGAGCAACTGGTCCACAAAATGTAAACGTTGCACTCGGTGTGGACAATCAGTGGGTGAACGGAGGGCAAGCTGAGGTGAGTGACAACAGATGGCATGAAATTGGTGGTTCCTTTAGAATTGAGAAGCAGCCATCCAAAGTTATGGTGTATATCCAAGGTCCTACTTCAGGTGTAGACTTAATGGTTGCTGGACTTCAGATATTTCCTGTTGACAGACAAGCAAGATTTAGATACTTGAAGAGGCAGACTGAGAAGATCCGTAAGCGCGATGTTGTCCTGAAATTTTCAGGATTAGACTGGAGCAGCGCATATGGCAGCTTCGTGAAAATTAAACAATCACAAAACAGTTTTCCATTTGGGACATGCATAAGCAGAACAAACATTGACAATGAAGATTTTGTTGATTTCTTCGTGAAAAACTTTAACTGGTCTGTATTTGGAAATGAACTGAAGTGGTATTGGACTGAACCTCAGAAGGGAAATTTCAATTACAAGGATGCCGATGAGATGGTGGACTTGTGCAAGAGTCACAACATAGATATGCGTGGCCATTGTATCTATTGGGAAGTGGTGGACACGGTCCAGCAATGGATCCGTTCATTGAGCCAAAATGACTTGGCAACTGCTGTCCAAAGTCGAGTAACAGACCTCCTCACCCGCTACAAAGGGAAGTTCCGGCACTATGATGTCAATAATGAGATGCTGCATGGTTCATTCTATCAAGACAAATTGGGTAAAGATGTCCGAGCCAACATGTTCAAGATGGCAAACCAATTGGATCCATCTGCACTCCTATTTGTGAATGATTATCATGTTGAGGATGGATGTGATACCAGATCAGTACCAGAAAAGTACATTGAGCAGATCCTTGATTTGCAACTACAAGGTGCCCCTGTTGGTGGGATTGGAATTCAAGGACATATAGATAGTCCAGTAGGTCCGATTGTTTGTTCCGCTCTTGATAAATTGGGTATCCTTGGCCTTCCAATCTGGTTCACAGAACTTGATGTGTCATCGAGCAATGAATGTGTTCGTGCAGATGACTTGGAAGTGATGCTTAGGGAAGCATTCGCCAATCCTTCAGTAGAAGGCATAGTCCTGTGGGGTTTCTGGGAGCTGTTCATGAGTCGTGAAAACTCCCATCTAGTGAATGCCGAAGGGGACATCAACGAAGCTGGAAAGCGTTACTTGGAACTCAAGCAAGAGTGGCTATCTCATGCTCATGGGCACATTGATGAACAGGGACAGTTCACGTTTAGAGGCTTCCATGGAACATACAGTGTAGAGATCGCTACACCCTCCAAGAAAGTTCTGAAGACATTTGTTGTTGACAAGGGTGATGACTCTCCATTTGAGGTTTCCATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

893

Amino Acids

98.53

Weight (kDa)

6.11

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 11 - 147 3.9e-19 Carbohydrate binding domain
CBM_4_9 PF02018 178 - 316 1.2e-19 Carbohydrate binding domain
CBM_4_9 PF02018 349 - 492 3.9e-17 Carbohydrate binding domain
Glyco_hydro_10 PF00331 550 - 806 1.5e-46 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
Acc65I GGTACC 1 cut(s) 26
AccB1I GGYRCC 4 cut(s) 26, 75, 1076, 2195
AccB7I CCANNNNNTGG 1 cut(s) 1086
AccBSI CCGCTC 1 cut(s) 2264
AccI GTMKAC 1 cut(s) 1482
AccII CGCG 1 cut(s) 1575
AccIII TCCGGA 1 cut(s) 753
AciI CCGC 5 cut(s) 239, 502, 1133, 1966, 2262
AclI AACGTT 1 cut(s) 1335
AclWI GGATC 8 cut(s) 900, 913, 1558, 1894, 1907, 2081, 2094, 2167
AcoI YGGCCR 2 cut(s) 118, 1855
AcsI RAATTY 7 cut(s) 133, 386, 551, 890, 1589, 1789, 2217
AcuI CTGAAG 5 cut(s) 1458, 1487, 1778, 2373, 2642
AdeI CACNNNGTG 1 cut(s) 1346
AfaI GTAC 5 cut(s) 28, 789, 852, 2151, 2162
AflIII ACRYGT 1 cut(s) 2061
AgsI TTSAA 8 cut(s) 12, 298, 883, 1261, 1546, 1795, 2068, 2222
AjnI CCWGG 4 cut(s) 217, 285, 309, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 819, 851
AluBI AGCT 8 cut(s) 110, 681, 727, 731, 1379, 1626, 2425, 2483
AluI AGCT 8 cut(s) 110, 681, 727, 731, 1379, 1626, 2425, 2483
AlwI GGATC 8 cut(s) 900, 913, 1558, 1894, 1907, 2081, 2094, 2167
AlwNI CAGNNNCTG 1 cut(s) 953
Aor13HI TCCGGA 1 cut(s) 753
AoxI GGCC 4 cut(s) 118, 434, 1855, 2287
ApeKI GCWGC 8 cut(s) 64, 588, 656, 659, 1435, 1611, 1623, 2011
ApoI RAATTY 7 cut(s) 133, 386, 551, 890, 1589, 1789, 2217
ArsI GACNNNNNNTTYG 2 cut(s) 2642, 2674
Asp700I GAANNNNTTC 2 cut(s) 1126, 2337
Asp718I GGTACC 1 cut(s) 26
AspLEI GCGC 5 cut(s) 1172, 1215, 1307, 1575, 1616
AspS9I GGNCC 8 cut(s) 260, 434, 938, 1232, 1319, 1466, 1888, 2246
AsuHPI GGTGA 8 cut(s) 539, 804, 1122, 1262, 1375, 1396, 1954, 2657
AsuNHI GCTAGC 1 cut(s) 727
AvaII GGWCC 7 cut(s) 260, 938, 1232, 1319, 1466, 1888, 2246
BaeGI GKGCMC 3 cut(s) 877, 2200, 2538
BalI TGGCCA 2 cut(s) 120, 1857
BamHI GGATCC 3 cut(s) 905, 1899, 2086
BanI GGYRCC 4 cut(s) 26, 75, 1076, 2195
BauI CACGAG 2 cut(s) 266, 775
BbsI GAAGAC 1 cut(s) 2630
BbvCI CCTCAGC 1 cut(s) 1380
BbvI GCAGC 8 cut(s) 51, 575, 643, 646, 1447, 1623, 1635, 1998
BcgI CGANNNNNNTGC 2 cut(s) 725, 759
BciT130I CCWGG 4 cut(s) 219, 287, 311, 444
BciVI GTATCC 1 cut(s) 2291
BfaI CTAG 2 cut(s) 728, 2453
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 2 cut(s) 1173, 1308
BfuI GTATCC 1 cut(s) 2291
Bme1390I CCNGG 4 cut(s) 219, 287, 311, 444
Bme18I GGWCC 7 cut(s) 260, 938, 1232, 1319, 1466, 1888, 2246
BmgT120I GGNCC 8 cut(s) 260, 434, 938, 1232, 1319, 1466, 1888, 2246
BmrFI CCNGG 4 cut(s) 219, 287, 311, 444
BmrI ACTGGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 1795, 1998, 2352
BmtI GCTAGC 1 cut(s) 731
BmuI ACTGGG 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 2630
BpmI CTGGAG 2 cut(s) 1329, 1627
Bpu10I CCTNAGC 2 cut(s) 1380, 2366
BpuEI CTTGAG 1 cut(s) 2489
BsaBI GATNNNNATC 1 cut(s) 910
BsaJI CCNNGG 6 cut(s) 217, 310, 1165, 1462, 2284, 2575
BsaWI WCCGGW 1 cut(s) 753
Bse1I ACTGG 9 cut(s) 208, 516, 583, 712, 1129, 1321, 1610, 1739, 2240
Bse3DI GCAATG 3 cut(s) 1901, 2335, 2670
Bse8I GATNNNNATC 1 cut(s) 910
BseAI TCCGGA 1 cut(s) 753
BseBI CCWGG 4 cut(s) 219, 287, 311, 444
BseDI CCNNGG 6 cut(s) 217, 310, 1165, 1462, 2284, 2575
BseGI GGATG 9 cut(s) 278, 565, 911, 1197, 1233, 1439, 1810, 2134, 2138
BseJI GATNNNNATC 1 cut(s) 910
BseMI GCAATG 3 cut(s) 1901, 2335, 2670
BseMII CTCAG 4 cut(s) 1253, 1371, 1548, 1793
BseNI ACTGG 9 cut(s) 208, 516, 583, 712, 1129, 1321, 1610, 1739, 2240
BseRI GAGGAG 1 cut(s) 1949
BseSI GKGCMC 3 cut(s) 877, 2200, 2538
BseXI GCAGC 8 cut(s) 51, 575, 643, 646, 1447, 1623, 1635, 1998
BsgI GTGCAG 4 cut(s) 50, 827, 2079, 2364
Bsh1236I CGCG 1 cut(s) 1575
BshFI GGCC 4 cut(s) 120, 436, 1857, 2289
BshNI GGYRCC 4 cut(s) 26, 75, 1076, 2195
BsiSI CCGG 2 cut(s) 754, 1984
BslFI GGGAC 8 cut(s) 199, 273, 510, 617, 983, 1681, 2483, 2565
BsmFI GGGAC 8 cut(s) 199, 273, 510, 617, 983, 1681, 2483, 2565
BsmI GAATGC 5 cut(s) 179, 311, 690, 2375, 2464
BsnI GGCC 4 cut(s) 120, 436, 1857, 2289
Bsp1286I GDGCHC 3 cut(s) 877, 2200, 2538
Bsp13I TCCGGA 1 cut(s) 753
Bsp143I GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
Bsp19I CCATGG 1 cut(s) 2575
BspACI CCGC 5 cut(s) 239, 502, 1133, 1966, 2262
BspANI GGCC 4 cut(s) 120, 436, 1857, 2289
BspCNI CTCAG 4 cut(s) 1252, 1372, 1549, 1792
BspEI TCCGGA 1 cut(s) 753
BspFNI CGCG 1 cut(s) 1575
BspHI TCATGA 2 cut(s) 1174, 2430
BspOI GCTAGC 1 cut(s) 731
BspPI GGATC 8 cut(s) 900, 913, 1558, 1894, 1907, 2081, 2094, 2167
BspT107I GGYRCC 4 cut(s) 26, 75, 1076, 2195
BsrBI CCGCTC 1 cut(s) 2264
BsrDI GCAATG 3 cut(s) 1901, 2335, 2670
BsrI ACTGG 9 cut(s) 208, 516, 583, 712, 1129, 1321, 1610, 1739, 2240
BssECI CCNNGG 6 cut(s) 217, 310, 1165, 1462, 2284, 2575
BssMI GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
BssSI CACGAG 2 cut(s) 266, 775
BssT1I CCWWGG 4 cut(s) 1165, 1462, 2284, 2575
Bst2BI CACGAG 2 cut(s) 266, 775
Bst2UI CCWGG 4 cut(s) 219, 287, 311, 444
Bst4CI ACNGT 9 cut(s) 396, 831, 879, 971, 1061, 1655, 1888, 2556, 2588
Bst6I CTCTTC 3 cut(s) 577, 706, 1541
BstC8I GCNNGC 4 cut(s) 179, 729, 987, 1377
BstDEI CTNAG 8 cut(s) 334, 846, 1067, 1239, 1380, 1557, 1779, 2366
BstDSI CCRYGG 1 cut(s) 2575
BstENI CCTNNNNNAGG 1 cut(s) 1473
BstF5I GGATG 9 cut(s) 278, 565, 911, 1197, 1233, 1439, 1810, 2134, 2138
BstFNI CGCG 1 cut(s) 1575
BstH2I RGCGCY 2 cut(s) 1173, 1308
BstHHI GCGC 5 cut(s) 1172, 1215, 1307, 1575, 1616
BstKTI GATC 8 cut(s) 477, 908, 1566, 1902, 2089, 2147, 2175, 2598
BstMBI GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
BstMWI GCNNNNNNNGC 7 cut(s) 73, 678, 687, 724, 737, 1620, 2515
BstNI CCWGG 4 cut(s) 219, 287, 311, 444
BstNSI RCATGY 2 cut(s) 1674, 2065
BstSCI CCNGG 4 cut(s) 217, 285, 309, 442
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 3 cut(s) 877, 2200, 2538
BstUI CGCG 1 cut(s) 1575
BstV1I GCAGC 8 cut(s) 51, 575, 643, 646, 1447, 1623, 1635, 1998
BstV2I GAAGAC 1 cut(s) 2630
BstX2I RGATCY 5 cut(s) 905, 1563, 1899, 2086, 2172
BstXI CCANNNNNNTGG 1 cut(s) 1450
BstYI RGATCY 5 cut(s) 905, 1563, 1899, 2086, 2172
BsuI GTATCC 1 cut(s) 2291
BsuRI GGCC 4 cut(s) 120, 436, 1857, 2289
BtgI CCRYGG 1 cut(s) 2575
BtgZI GCGATG 1 cut(s) 1590
BtsCI GGATG 9 cut(s) 278, 565, 911, 1197, 1233, 1439, 1810, 2134, 2138
BtsIMutI CAGTG 5 cut(s) 201, 392, 523, 1364, 2593
Cac8I GCNNGC 4 cut(s) 179, 729, 987, 1377
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 2 cut(s) 1174, 2430
CfoI GCGC 5 cut(s) 1172, 1215, 1307, 1575, 1616
Cfr13I GGNCC 8 cut(s) 260, 434, 938, 1232, 1319, 1466, 1888, 2246
Csp6I GTAC 5 cut(s) 27, 788, 851, 2150, 2161
CviQI GTAC 5 cut(s) 27, 788, 851, 2150, 2161
DdeI CTNAG 8 cut(s) 334, 846, 1067, 1239, 1380, 1557, 1779, 2366
DpnI GATC 8 cut(s) 476, 907, 1565, 1901, 2088, 2146, 2174, 2597
DpnII GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
DraI TTTAAA 1 cut(s) 900
DraIII CACNNNGTG 1 cut(s) 1346
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 2 cut(s) 118, 1855
Eam1104I CTCTTC 3 cut(s) 577, 706, 1541
EarI CTCTTC 3 cut(s) 577, 706, 1541
Eco130I CCWWGG 4 cut(s) 1165, 1462, 2284, 2575
Eco32I GATATC 1 cut(s) 199
Eco47I GGWCC 7 cut(s) 260, 938, 1232, 1319, 1466, 1888, 2246
Eco57I CTGAAG 5 cut(s) 1458, 1487, 1778, 2373, 2642
EcoNI CCTNNNNNAGG 1 cut(s) 1473
EcoO109I RGGNCCY 4 cut(s) 260, 434, 1232, 1466
EcoRI GAATTC 1 cut(s) 2217
EcoRII CCWGG 4 cut(s) 217, 285, 309, 442
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 4 cut(s) 1165, 1462, 2284, 2575
EcoT22I ATGCAT 1 cut(s) 1676
ErhI CCWWGG 4 cut(s) 1165, 1462, 2284, 2575
FalI AAGNNNNNCTT 6 cut(s) 317, 349, 2349, 2381, 2480, 2512
FaqI GGGAC 8 cut(s) 199, 273, 510, 617, 983, 1681, 2483, 2565
FauI CCCGC 1 cut(s) 1973
FauNDI CATATG 1 cut(s) 1618
FblI GTMKAC 1 cut(s) 1482
FokI GGATG 9 cut(s) 285, 572, 898, 1184, 1240, 1426, 1817, 2141, 2145
FspBI CTAG 2 cut(s) 728, 2453
GlaI GCGC 5 cut(s) 1171, 1214, 1306, 1574, 1615
GsuI CTGGAG 2 cut(s) 1329, 1627
HaeII RGCGCY 2 cut(s) 1173, 1308
HaeIII GGCC 4 cut(s) 120, 436, 1857, 2289
HapII CCGG 2 cut(s) 754, 1984
HhaI GCGC 5 cut(s) 1172, 1215, 1307, 1575, 1616
Hin6I GCGC 5 cut(s) 1170, 1213, 1305, 1573, 1614
HinP1I GCGC 5 cut(s) 1170, 1213, 1305, 1573, 1614
HincII GTYRAC 4 cut(s) 399, 967, 1519, 2638
HindII GTYRAC 4 cut(s) 399, 967, 1519, 2638
HinfI GANTC 5 cut(s) 608, 1027, 1834, 2434, 2651
HpaII CCGG 2 cut(s) 754, 1984
HphI GGTGA 8 cut(s) 539, 804, 1122, 1262, 1375, 1396, 1954, 2657
HpyCH4III ACNGT 9 cut(s) 396, 831, 879, 971, 1061, 1655, 1888, 2556, 2588
HpyCH4IV ACGT 2 cut(s) 1335, 2561
HpyF10VI GCNNNNNNNGC 7 cut(s) 73, 678, 687, 724, 737, 1620, 2515
HpyF3I CTNAG 8 cut(s) 334, 846, 1067, 1239, 1380, 1557, 1779, 2366
HpySE526I ACGT 2 cut(s) 1335, 2561
HspAI GCGC 5 cut(s) 1170, 1213, 1305, 1573, 1614
Kpn2I TCCGGA 1 cut(s) 753
KpnI GGTACC 1 cut(s) 30
Kzo9I GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
LmnI GCTCC 4 cut(s) 190, 1310, 1608, 2422
Lsp1109I GCAGC 8 cut(s) 51, 575, 643, 646, 1447, 1623, 1635, 1998
LweI GCATC 3 cut(s) 1795, 1998, 2352
MaeI CTAG 2 cut(s) 728, 2453
MaeII ACGT 2 cut(s) 1335, 2561
MalI GATC 8 cut(s) 476, 907, 1565, 1901, 2088, 2146, 2174, 2597
MbiI CCGCTC 1 cut(s) 2264
MboI GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
MboII GAAGA 8 cut(s) 594, 723, 999, 1558, 1573, 1708, 1712, 2635
MfeI CAATTG 2 cut(s) 82, 2081
MflI RGATCY 5 cut(s) 905, 1563, 1899, 2086, 2172
MhlI GDGCHC 3 cut(s) 877, 2200, 2538
MlsI TGGCCA 2 cut(s) 120, 1857
MluNI TGGCCA 2 cut(s) 120, 1857
MlyI GAGTC 4 cut(s) 602, 1843, 2443, 2645
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 2 cut(s) 120, 1857
Mph1103I ATGCAT 1 cut(s) 1676
MroI TCCGGA 1 cut(s) 753
MroXI GAANNNNTTC 2 cut(s) 1126, 2337
MscI TGGCCA 2 cut(s) 120, 1857
MseI TTAA 6 cut(s) 6, 899, 1184, 1487, 1638, 1731
MslI CAYNNNNRTG 5 cut(s) 510, 1847, 1992, 2529, 2541
Msp20I TGGCCA 2 cut(s) 120, 1857
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 2 cut(s) 754, 1984
MspR9I CCNGG 4 cut(s) 219, 287, 311, 444
MunI CAATTG 2 cut(s) 82, 2081
Mva1269I GAATGC 5 cut(s) 179, 311, 690, 2375, 2464
MvaI CCWGG 4 cut(s) 219, 287, 311, 444
MvnI CGCG 1 cut(s) 1575
MwoI GCNNNNNNNGC 7 cut(s) 73, 678, 687, 724, 737, 1620, 2515
NcoI CCATGG 1 cut(s) 2575
NdeI CATATG 1 cut(s) 1618
NdeII GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
NheI GCTAGC 1 cut(s) 727
NmuCI GTSAC 6 cut(s) 527, 772, 810, 1250, 1388, 1835
NsiI ATGCAT 1 cut(s) 1676
NspI RCATGY 2 cut(s) 1674, 2065
PagI TCATGA 2 cut(s) 1174, 2430
PciI ACATGT 1 cut(s) 2061
PctI GAATGC 5 cut(s) 179, 311, 690, 2375, 2464
PdmI GAANNNNTTC 2 cut(s) 1126, 2337
PfeI GAWTC 1 cut(s) 1027
PflFI GACNNNGTC 1 cut(s) 1886
PflMI CCANNNNNTGG 1 cut(s) 1086
PfoI TCCNGGA 1 cut(s) 442
PleI GAGTC 4 cut(s) 602, 1842, 2442, 2645
PpsI GAGTC 4 cut(s) 602, 1842, 2442, 2645
PpuMI RGGWCCY 3 cut(s) 260, 1232, 1466
PscI ACATGT 1 cut(s) 2061
Psp1406I AACGTT 1 cut(s) 1335
Psp5II RGGWCCY 3 cut(s) 260, 1232, 1466
Psp6I CCWGG 4 cut(s) 217, 285, 309, 442
PspGI CCWGG 4 cut(s) 217, 285, 309, 442
PspPI GGNCC 8 cut(s) 260, 434, 938, 1232, 1319, 1466, 1888, 2246
PspPPI RGGWCCY 3 cut(s) 260, 1232, 1466
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 5 cut(s) 905, 1563, 1899, 2086, 2172
PsyI GACNNNGTC 1 cut(s) 1886
RsaI GTAC 5 cut(s) 28, 789, 852, 2151, 2162
RsaNI GTAC 5 cut(s) 27, 788, 851, 2150, 2161
RseI CAYNNNNRTG 5 cut(s) 510, 1847, 1992, 2529, 2541
SaqAI TTAA 6 cut(s) 6, 899, 1184, 1487, 1638, 1731
Sau3AI GATC 8 cut(s) 474, 905, 1563, 1899, 2086, 2144, 2172, 2595
Sau96I GGNCC 8 cut(s) 260, 434, 938, 1232, 1319, 1466, 1888, 2246
SchI GAGTC 4 cut(s) 602, 1843, 2443, 2645
ScrFI CCNGG 4 cut(s) 219, 287, 311, 444
SduI GDGCHC 3 cut(s) 877, 2200, 2538
SfaNI GCATC 3 cut(s) 1795, 1998, 2352
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 7 cut(s) 260, 938, 1232, 1319, 1466, 1888, 2246
SmiMI CAYNNNNRTG 5 cut(s) 510, 1847, 1992, 2529, 2541
SmlI CTYRAG 1 cut(s) 2504
SmoI CTYRAG 1 cut(s) 2504
SsiI CCGC 5 cut(s) 239, 502, 1133, 1966, 2262
SspMI CTAG 2 cut(s) 728, 2453
StyD4I CCNGG 4 cut(s) 217, 285, 309, 442
StyI CCWWGG 4 cut(s) 1165, 1462, 2284, 2575
TaaI ACNGT 9 cut(s) 396, 831, 879, 971, 1061, 1655, 1888, 2556, 2588
TaiI ACGT 2 cut(s) 1338, 2564
TaqI TCGA 3 cut(s) 611, 1945, 2324
TatI WGTACW 1 cut(s) 2160
TauI GCSGC 2 cut(s) 242, 505
TfiI GAWTC 1 cut(s) 1027
Tru1I TTAA 6 cut(s) 6, 899, 1184, 1487, 1638, 1731
Tru9I TTAA 6 cut(s) 6, 899, 1184, 1487, 1638, 1731
TscAI CASTG 5 cut(s) 208, 399, 523, 1364, 2593
TseFI GTSAC 6 cut(s) 527, 772, 810, 1250, 1388, 1835
TseI GCWGC 8 cut(s) 64, 588, 656, 659, 1435, 1611, 1623, 2011
Tsp45I GTSAC 6 cut(s) 527, 772, 810, 1250, 1388, 1835
TspGWI ACGGA 5 cut(s) 522, 918, 1383, 1556, 1892
TspRI CASTG 5 cut(s) 208, 399, 523, 1364, 2593
Tth111I GACNNNGTC 1 cut(s) 1886
Van91I CCANNNNNTGG 1 cut(s) 1086
VpaK11BI GGWCC 7 cut(s) 260, 938, 1232, 1319, 1466, 1888, 2246
XagI CCTNNNNNAGG 1 cut(s) 1473
XapI RAATTY 7 cut(s) 133, 386, 551, 890, 1589, 1789, 2217
XceI RCATGY 2 cut(s) 1674, 2065
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XmiI GTMKAC 1 cut(s) 1482
XmnI GAANNNNTTC 2 cut(s) 1126, 2337
XspI CTAG 2 cut(s) 728, 2453
Zsp2I ATGCAT 1 cut(s) 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.