Rroxscaffold_7G00158670

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006231
Physical Location & Seq
Forward (+)
2013797 .. 2018251
4455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158670.1

Sequence Viewer

Length: 2739 bp
ATGGAGAACCAAACGATTGAAAATGCCGCCGACGATAACCAGATTGTGAAACAGGATATGGTTAATTCAAGTACTAGTCATGGTACCAATATCATAGTGAACCATGATTTCTGCGGAGGGCTGCACTCTTGGCACCCCAATTGCTGTGAGGGATATGTAGTTTCAGCTGATTCTGGCCACCCACAAGCAAATTCGGGTGGCAATTATGCAGTTGTTACTAATCGGAAAGAATGCTGGCAAGGATTGGAGCAGGATATCACTGGGAGGGTGTCCCCTGGTTTGACTTACTTGGTTTCGGCTTCTGTTGGGGTTTCAGGACCTCTCGTCGGATGTGTTGATGTCCTGGCAACATTGAAAATGGAATGCCAGGGTTCAGAAACTAAGTATTCGTTAGTTGGGAGAAGTCCTGTATCAAATGGGAAATGGGAAAAGTTGGAAGGCAAATTTTCACTGTCAACAATGCCTGATAAGGTTGTGTTTTACTTGGAAGGGCCTTCTCCTGGAATTGACTTGCTTATACAATCTGTAGTGATCTCTTGTTCCAATCCAAAGGGATGGCGGCACGGAAATGCCAGTGATGGAGATGAAGATATAGTCCTAAACCCAAAATTTGAGGATGGCTTGACTAATTGGACTGGAAGAGGCTGCAAGGTAGTTCTACACGACTCAATGGGAGATGGGAAAATCGTCCCTCAATCTGGAAAAGTTTTTGCTGCTGCAACAGAACGCACACAGAGCTGGAATGGCATTCAGCAGGATATTACTGGAAGAGTGCAACGAAAGTTAGCTTACGAGGCTACTGCTGTTGTCCGGATATTTGGTAACAATGTCACGGGTTCTGATGTACGGGCTACCTTATGGGTGCAGTCACCAAATGGTCGTGAACAGTATATTGGCATTTCTAAGGTACAGGCAACAGACAAGGATTGGGCACAGTTGAAGGGGAAATTCCTTTTAAACGGATCCCCATCCAAAGTAGTCGTTTATTTAGAAGGTCCACCAGCAGGAACTGATATTCTTGTCAACAGTTTCGTTGTCAAGCACGCACAGAAAGTCCCTCCTTCTTCTCCACCAGATATTGAGAATCCTGCTTTTGGAGTAAATATAATTGAGAACAGTAATCAAAGTAATGGCACCAATGGGTGGTTTCCTCTTGGTAATTGCACTTTGAGTGTGGGAACTGGTTCACCGCATATACTTCCTCCAATGGCGAGAGATAGCCTTGGCGCTCATGAACCTTTAAGTGGTCGCTTCATCCTTGTAACCAAGCGCACACAGACTTGGATGGGTCCTGCTCAGATGATTGGTGACAAATTGAAACTCTTTTTGACATATCAAGTATCTGCTTGGGTTCGGATTGGCGCTGGAGCAACTGGTCCACAAAATGTGAACGTTGCACTCGGTGTGGACAATCAGTGGGTGAACGGAGGGCAAGCTGAGGTGGGTGACAACAGATGGCATGAAATTGGTGGTTCCTTTAGAATTGAGAAGCAGCCATCCAAAGTTATGGTGTATATCCAAGGTCCTGCTTCAGGTGTAGACTTAATGGTTGCTGGACTTCAGATATTTCCTGTTGACAGAAAAGCAAGATTTAGATACTTGAAGAGGCAGACTGAGAAGATCCGTAAGCGCGATGTTGTCCTGAAATTTTCAGGATTAGACTGGAGCAGCGCATATGGCAGCTTCGTGAAAATTAAACAATCACAAAACAGTTTTCCATTTGGGACATGCATAAGCAGAACAAACATTGACAATGAAGATTTTGTTGATTTCTTTGTGAAAAGCTTTAACTGGTCTGTATTTGGAAATGAACTGAAGTGGTATTGGACTGAGCCTCAGAAGGGAAATTTCAATTACAAGGATGCCGATGAGATGGTGGACTTGTGCAAGAGTCACAACATAGATATGCGTGGCCATTGTATCTATTGGGAAGTGGTGGACACGGTCCAGCAATGGATCCGTTCATTGAGCCAAAATGACTTGGCAACTGCTGTCCAAAGTCGAGTAACAGACCTCCTCACCCGCTACAAAGGGAAGTTCCGGCACTATGATGTCAATAATGAGATGCTGCATGGTTCATTCTATCAAGACAAATTGGGTAAAGATATCCGACCCAACATGTTCAAGATGGCAAACCAATTGGATCCATCTGCACTCCTATTTGTGAATGATTATCATGTCGAGGATGGATGTGATACCAGATCAGCACCAGAAAAGTACATTGAACAGATCCTTGATCTGCAACTACAAGGTGCCCCTGTTGGTGGGATTGGAATTCAAGGACATATAGATAGTCCAGTAGGTCCAATTGTTTGTTCGGCTCTTGATAAATTGGGTATCCTTGGCCTTCCAATCTGGTTCACAGAACTTGATGTGTCATCAAGCAATGAATATGTTCGTGCGGATGACTTGGAAGTGATGCTTAGGGAAGCATTTGCCAATCCTTCAGTAGAAGGCATAGTCCTGTGGGGTTTCTGGGAGCTGTTCATGAGTCGTGAAAACTCCCATCTAGTGAATGCCGAAGGGGACATCAACGAAGCTGGAAAGCGTTACTTGGAACTCAAGCAAGAGTGGCTATCTCATGCTCATGGGCACATTGATGAACAGGGACAGTTCACGTTTAGAGGCTTCCATGGAACATACAGTGTAGAGATCGCTACCCCCTCCAAGAAAGTTCTGAAGACATTTGTTGTTGACAAGGGTGATGACTCTCCATTTGAGGTTTCCATTGCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

912

Amino Acids

100.81

Weight (kDa)

5.81

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 30 - 166 1.7e-20 Carbohydrate binding domain
CBM_4_9 PF02018 197 - 335 3.8e-19 Carbohydrate binding domain
CBM_4_9 PF02018 368 - 511 4.9e-16 Carbohydrate binding domain
Glyco_hydro_10 PF00331 569 - 825 4e-46 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 83
AccB1I GGYRCC 4 cut(s) 83, 132, 1133, 2252
AccB7I CCANNNNNTGG 1 cut(s) 1143
AccI GTMKAC 1 cut(s) 1539
AccII CGCG 1 cut(s) 1632
AccIII TCCGGA 1 cut(s) 810
AciI CCGC 6 cut(s) 27, 114, 559, 1190, 2023, 2402
AclI AACGTT 1 cut(s) 1392
AclWI GGATC 8 cut(s) 957, 970, 1615, 1951, 1964, 2138, 2151, 2224
AcoI YGGCCR 2 cut(s) 175, 1912
AcsI RAATTY 7 cut(s) 190, 443, 608, 947, 1646, 1846, 2274
AcuI CTGAAG 5 cut(s) 1515, 1544, 1835, 2430, 2699
AdeI CACNNNGTG 1 cut(s) 1403
AfaI GTAC 5 cut(s) 73, 85, 846, 909, 2219
AflIII ACRYGT 1 cut(s) 2118
AhdI GACNNNNNGTC 1 cut(s) 323
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 4 cut(s) 274, 342, 366, 499
AjuI GAANNNNNNNTTGG 4 cut(s) 876, 908, 2299, 2331
AluBI AGCT 8 cut(s) 167, 738, 788, 1436, 1683, 1785, 2482, 2540
AluI AGCT 8 cut(s) 167, 738, 788, 1436, 1683, 1785, 2482, 2540
AlwI GGATC 8 cut(s) 957, 970, 1615, 1951, 1964, 2138, 2151, 2224
AlwNI CAGNNNCTG 1 cut(s) 1010
Aor13HI TCCGGA 1 cut(s) 810
AoxI GGCC 4 cut(s) 175, 491, 1912, 2344
ApeKI GCWGC 8 cut(s) 121, 645, 713, 716, 1492, 1668, 1680, 2068
ApoI RAATTY 7 cut(s) 190, 443, 608, 947, 1646, 1846, 2274
ArsI GACNNNNNNTTYG 2 cut(s) 2699, 2731
Asp700I GAANNNNTTC 2 cut(s) 1183, 2394
Asp718I GGTACC 1 cut(s) 83
AspLEI GCGC 5 cut(s) 1229, 1272, 1364, 1632, 1673
AspS9I GGNCC 8 cut(s) 317, 491, 995, 1289, 1376, 1523, 1945, 2303
AsuHPI GGTGA 7 cut(s) 861, 1179, 1319, 1432, 1457, 2011, 2714
AvaII GGWCC 7 cut(s) 317, 995, 1289, 1376, 1523, 1945, 2303
BaeGI GKGCMC 3 cut(s) 934, 2257, 2595
BalI TGGCCA 2 cut(s) 177, 1914
BamHI GGATCC 3 cut(s) 962, 1956, 2143
BanI GGYRCC 4 cut(s) 83, 132, 1133, 2252
BbsI GAAGAC 1 cut(s) 2687
BbvCI CCTCAGC 1 cut(s) 1437
BbvI GCAGC 8 cut(s) 108, 632, 700, 703, 1504, 1680, 1692, 2055
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 4 cut(s) 276, 344, 368, 501
BciVI GTATCC 1 cut(s) 2348
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 2510
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 2 cut(s) 1230, 1365
BfuI GTATCC 1 cut(s) 2348
BmcAI AGTACT 1 cut(s) 73
Bme1390I CCNGG 4 cut(s) 276, 344, 368, 501
Bme18I GGWCC 7 cut(s) 317, 995, 1289, 1376, 1523, 1945, 2303
BmeRI GACNNNNNGTC 1 cut(s) 323
BmgT120I GGNCC 8 cut(s) 317, 491, 995, 1289, 1376, 1523, 1945, 2303
BmiI GGNNCC 9 cut(s) 85, 134, 964, 1135, 1290, 1474, 1958, 2145, 2254
BmrFI CCNGG 4 cut(s) 276, 344, 368, 501
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 3 cut(s) 1852, 2055, 2409
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 2687
BpmI CTGGAG 2 cut(s) 1386, 1684
Bpu10I CCTNAGC 2 cut(s) 1437, 2423
BpuEI CTTGAG 1 cut(s) 2546
BsaBI GATNNNNATC 1 cut(s) 967
BsaJI CCNNGG 6 cut(s) 274, 367, 1222, 1519, 2341, 2632
BsaWI WCCGGW 1 cut(s) 810
Bse1I ACTGG 9 cut(s) 265, 573, 640, 769, 1186, 1378, 1667, 1796, 2297
Bse3DI GCAATG 3 cut(s) 1958, 2392, 2727
Bse8I GATNNNNATC 1 cut(s) 967
BseAI TCCGGA 1 cut(s) 810
BseBI CCWGG 4 cut(s) 276, 344, 368, 501
BseDI CCNNGG 6 cut(s) 274, 367, 1222, 1519, 2341, 2632
BseJI GATNNNNATC 1 cut(s) 967
BseMI GCAATG 3 cut(s) 1958, 2392, 2727
BseMII CTCAG 5 cut(s) 1310, 1428, 1605, 1821, 1850
BseNI ACTGG 9 cut(s) 265, 573, 640, 769, 1186, 1378, 1667, 1796, 2297
BseRI GAGGAG 1 cut(s) 2006
BseSI GKGCMC 3 cut(s) 934, 2257, 2595
BseXI GCAGC 8 cut(s) 108, 632, 700, 703, 1504, 1680, 1692, 2055
BsgI GTGCAG 3 cut(s) 107, 884, 2136
Bsh1236I CGCG 1 cut(s) 1632
BshFI GGCC 4 cut(s) 177, 493, 1914, 2346
BshNI GGYRCC 4 cut(s) 83, 132, 1133, 2252
BsiSI CCGG 2 cut(s) 811, 2041
BslFI GGGAC 6 cut(s) 256, 674, 1040, 1738, 2540, 2622
BsmFI GGGAC 6 cut(s) 256, 674, 1040, 1738, 2540, 2622
BsmI GAATGC 4 cut(s) 236, 368, 747, 2521
BsnI GGCC 4 cut(s) 177, 493, 1914, 2346
Bsp1286I GDGCHC 3 cut(s) 934, 2257, 2595
Bsp13I TCCGGA 1 cut(s) 810
Bsp143I GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
Bsp19I CCATGG 1 cut(s) 2632
BspACI CCGC 6 cut(s) 27, 114, 559, 1190, 2023, 2402
BspANI GGCC 4 cut(s) 177, 493, 1914, 2346
BspCNI CTCAG 5 cut(s) 1309, 1429, 1606, 1822, 1849
BspEI TCCGGA 1 cut(s) 810
BspFNI CGCG 1 cut(s) 1632
BspHI TCATGA 2 cut(s) 1231, 2487
BspLI GGNNCC 9 cut(s) 85, 134, 964, 1135, 1290, 1474, 1958, 2145, 2254
BspPI GGATC 8 cut(s) 957, 970, 1615, 1951, 1964, 2138, 2151, 2224
BspT107I GGYRCC 4 cut(s) 83, 132, 1133, 2252
BsrDI GCAATG 3 cut(s) 1958, 2392, 2727
BsrI ACTGG 9 cut(s) 265, 573, 640, 769, 1186, 1378, 1667, 1796, 2297
BssECI CCNNGG 6 cut(s) 274, 367, 1222, 1519, 2341, 2632
BssMI GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
BssT1I CCWWGG 4 cut(s) 1222, 1519, 2341, 2632
Bst2UI CCWGG 4 cut(s) 276, 344, 368, 501
Bst4CI ACNGT 9 cut(s) 453, 888, 936, 1028, 1118, 1712, 1945, 2613, 2645
Bst6I CTCTTC 3 cut(s) 634, 763, 1598
BstC8I GCNNGC 3 cut(s) 236, 1044, 1434
BstDEI CTNAG 8 cut(s) 381, 903, 1296, 1437, 1614, 1830, 1836, 2423
BstDSI CCRYGG 1 cut(s) 2632
BstENI CCTNNNNNAGG 1 cut(s) 1530
BstFNI CGCG 1 cut(s) 1632
BstH2I RGCGCY 2 cut(s) 1230, 1365
BstHHI GCGC 5 cut(s) 1229, 1272, 1364, 1632, 1673
BstKTI GATC 9 cut(s) 534, 965, 1623, 1959, 2146, 2204, 2232, 2239, 2655
BstMBI GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
BstMWI GCNNNNNNNGC 6 cut(s) 130, 735, 744, 794, 1677, 2572
BstNI CCWGG 4 cut(s) 276, 344, 368, 501
BstNSI RCATGY 2 cut(s) 1731, 2122
BstSCI CCNGG 4 cut(s) 274, 342, 366, 499
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 3 cut(s) 934, 2257, 2595
BstUI CGCG 1 cut(s) 1632
BstV1I GCAGC 8 cut(s) 108, 632, 700, 703, 1504, 1680, 1692, 2055
BstV2I GAAGAC 1 cut(s) 2687
BstX2I RGATCY 5 cut(s) 962, 1620, 1956, 2143, 2229
BstXI CCANNNNNNTGG 2 cut(s) 555, 1507
BstYI RGATCY 5 cut(s) 962, 1620, 1956, 2143, 2229
BsuI GTATCC 1 cut(s) 2348
BsuRI GGCC 4 cut(s) 177, 493, 1914, 2346
BtgI CCRYGG 1 cut(s) 2632
BtgZI GCGATG 1 cut(s) 1647
BtsIMutI CAGTG 5 cut(s) 258, 449, 580, 1421, 2650
Cac8I GCNNGC 3 cut(s) 236, 1044, 1434
CaiI CAGNNNCTG 1 cut(s) 1010
CciI TCATGA 2 cut(s) 1231, 2487
CfoI GCGC 5 cut(s) 1229, 1272, 1364, 1632, 1673
Cfr13I GGNCC 8 cut(s) 317, 491, 995, 1289, 1376, 1523, 1945, 2303
Csp6I GTAC 5 cut(s) 72, 84, 845, 908, 2218
CviQI GTAC 5 cut(s) 72, 84, 845, 908, 2218
DdeI CTNAG 8 cut(s) 381, 903, 1296, 1437, 1614, 1830, 1836, 2423
DpnI GATC 9 cut(s) 533, 964, 1622, 1958, 2145, 2203, 2231, 2238, 2654
DpnII GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
DraI TTTAAA 1 cut(s) 957
DraIII CACNNNGTG 1 cut(s) 1403
DriI GACNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 2 cut(s) 175, 1912
Eam1104I CTCTTC 3 cut(s) 634, 763, 1598
Eam1105I GACNNNNNGTC 1 cut(s) 323
EarI CTCTTC 3 cut(s) 634, 763, 1598
Eco130I CCWWGG 4 cut(s) 1222, 1519, 2341, 2632
Eco32I GATATC 2 cut(s) 256, 2107
Eco47I GGWCC 7 cut(s) 317, 995, 1289, 1376, 1523, 1945, 2303
Eco57I CTGAAG 5 cut(s) 1515, 1544, 1835, 2430, 2699
EcoNI CCTNNNNNAGG 1 cut(s) 1530
EcoO109I RGGNCCY 4 cut(s) 317, 491, 1289, 1523
EcoRI GAATTC 1 cut(s) 2274
EcoRII CCWGG 4 cut(s) 274, 342, 366, 499
EcoRV GATATC 2 cut(s) 256, 2107
EcoT14I CCWWGG 4 cut(s) 1222, 1519, 2341, 2632
EcoT22I ATGCAT 1 cut(s) 1733
ErhI CCWWGG 4 cut(s) 1222, 1519, 2341, 2632
FalI AAGNNNNNCTT 4 cut(s) 2406, 2438, 2537, 2569
FaqI GGGAC 6 cut(s) 256, 674, 1040, 1738, 2540, 2622
FauI CCCGC 1 cut(s) 2030
FauNDI CATATG 1 cut(s) 1675
FblI GTMKAC 1 cut(s) 1539
FspBI CTAG 2 cut(s) 75, 2510
GlaI GCGC 5 cut(s) 1228, 1271, 1363, 1631, 1672
GsuI CTGGAG 2 cut(s) 1386, 1684
HaeII RGCGCY 2 cut(s) 1230, 1365
HaeIII GGCC 4 cut(s) 177, 493, 1914, 2346
HapII CCGG 2 cut(s) 811, 2041
HhaI GCGC 5 cut(s) 1229, 1272, 1364, 1632, 1673
Hin6I GCGC 5 cut(s) 1227, 1270, 1362, 1630, 1671
HinP1I GCGC 5 cut(s) 1227, 1270, 1362, 1630, 1671
HincII GTYRAC 4 cut(s) 456, 1024, 1576, 2695
HindII GTYRAC 4 cut(s) 456, 1024, 1576, 2695
HindIII AAGCTT 1 cut(s) 1783
HinfI GANTC 6 cut(s) 170, 665, 1084, 1891, 2491, 2708
HpaII CCGG 2 cut(s) 811, 2041
HphI GGTGA 7 cut(s) 861, 1179, 1319, 1432, 1457, 2011, 2714
Hpy99I CGWCG 2 cut(s) 35, 329
HpyCH4III ACNGT 9 cut(s) 453, 888, 936, 1028, 1118, 1712, 1945, 2613, 2645
HpyCH4IV ACGT 2 cut(s) 1392, 2618
HpyF10VI GCNNNNNNNGC 6 cut(s) 130, 735, 744, 794, 1677, 2572
HpyF3I CTNAG 8 cut(s) 381, 903, 1296, 1437, 1614, 1830, 1836, 2423
HpySE526I ACGT 2 cut(s) 1392, 2618
HspAI GCGC 5 cut(s) 1227, 1270, 1362, 1630, 1671
Kpn2I TCCGGA 1 cut(s) 810
KpnI GGTACC 1 cut(s) 87
Kzo9I GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
LmnI GCTCC 4 cut(s) 247, 1367, 1665, 2479
Lsp1109I GCAGC 8 cut(s) 108, 632, 700, 703, 1504, 1680, 1692, 2055
LweI GCATC 3 cut(s) 1852, 2055, 2409
MaeI CTAG 2 cut(s) 75, 2510
MaeII ACGT 2 cut(s) 1392, 2618
MalI GATC 9 cut(s) 533, 964, 1622, 1958, 2145, 2203, 2231, 2238, 2654
MboI GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
MboII GAAGA 8 cut(s) 599, 651, 780, 1056, 1615, 1630, 1769, 2692
MfeI CAATTG 3 cut(s) 139, 2138, 2307
MflI RGATCY 5 cut(s) 962, 1620, 1956, 2143, 2229
MhlI GDGCHC 3 cut(s) 934, 2257, 2595
MlsI TGGCCA 2 cut(s) 177, 1914
MluNI TGGCCA 2 cut(s) 177, 1914
MlyI GAGTC 4 cut(s) 659, 1900, 2500, 2702
MmeI TCCRAC 3 cut(s) 307, 414, 2134
Mox20I TGGCCA 2 cut(s) 177, 1914
Mph1103I ATGCAT 1 cut(s) 1733
MroI TCCGGA 1 cut(s) 810
MroXI GAANNNNTTC 2 cut(s) 1183, 2394
MscI TGGCCA 2 cut(s) 177, 1914
MseI TTAA 6 cut(s) 63, 956, 1241, 1544, 1695, 1788
MslI CAYNNNNRTG 5 cut(s) 567, 1904, 2049, 2586, 2598
Msp20I TGGCCA 2 cut(s) 177, 1914
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 2 cut(s) 811, 2041
MspR9I CCNGG 4 cut(s) 276, 344, 368, 501
MunI CAATTG 3 cut(s) 139, 2138, 2307
Mva1269I GAATGC 4 cut(s) 236, 368, 747, 2521
MvaI CCWGG 4 cut(s) 276, 344, 368, 501
MvnI CGCG 1 cut(s) 1632
MwoI GCNNNNNNNGC 6 cut(s) 130, 735, 744, 794, 1677, 2572
NcoI CCATGG 1 cut(s) 2632
NdeI CATATG 1 cut(s) 1675
NdeII GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
NlaIV GGNNCC 9 cut(s) 85, 134, 964, 1135, 1290, 1474, 1958, 2145, 2254
NmuCI GTSAC 5 cut(s) 829, 867, 1307, 1445, 1892
NsiI ATGCAT 1 cut(s) 1733
NspI RCATGY 2 cut(s) 1731, 2122
PagI TCATGA 2 cut(s) 1231, 2487
PciI ACATGT 1 cut(s) 2118
PctI GAATGC 4 cut(s) 236, 368, 747, 2521
PdmI GAANNNNTTC 2 cut(s) 1183, 2394
PfeI GAWTC 2 cut(s) 170, 1084
PflFI GACNNNGTC 1 cut(s) 1943
PflMI CCANNNNNTGG 1 cut(s) 1143
PfoI TCCNGGA 1 cut(s) 499
PleI GAGTC 4 cut(s) 659, 1899, 2499, 2702
PpsI GAGTC 4 cut(s) 659, 1899, 2499, 2702
PpuMI RGGWCCY 3 cut(s) 317, 1289, 1523
PscI ACATGT 1 cut(s) 2118
Psp1406I AACGTT 1 cut(s) 1392
Psp5II RGGWCCY 3 cut(s) 317, 1289, 1523
Psp6I CCWGG 4 cut(s) 274, 342, 366, 499
PspGI CCWGG 4 cut(s) 274, 342, 366, 499
PspN4I GGNNCC 9 cut(s) 85, 134, 964, 1135, 1290, 1474, 1958, 2145, 2254
PspPI GGNCC 8 cut(s) 317, 491, 995, 1289, 1376, 1523, 1945, 2303
PspPPI RGGWCCY 3 cut(s) 317, 1289, 1523
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 5 cut(s) 962, 1620, 1956, 2143, 2229
PsyI GACNNNGTC 1 cut(s) 1943
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 5 cut(s) 73, 85, 846, 909, 2219
RsaNI GTAC 5 cut(s) 72, 84, 845, 908, 2218
RseI CAYNNNNRTG 5 cut(s) 567, 1904, 2049, 2586, 2598
SaqAI TTAA 6 cut(s) 63, 956, 1241, 1544, 1695, 1788
Sau3AI GATC 9 cut(s) 531, 962, 1620, 1956, 2143, 2201, 2229, 2236, 2652
Sau96I GGNCC 8 cut(s) 317, 491, 995, 1289, 1376, 1523, 1945, 2303
ScaI AGTACT 1 cut(s) 73
SchI GAGTC 4 cut(s) 659, 1900, 2500, 2702
ScrFI CCNGG 4 cut(s) 276, 344, 368, 501
SduI GDGCHC 3 cut(s) 934, 2257, 2595
SfaNI GCATC 3 cut(s) 1852, 2055, 2409
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 7 cut(s) 317, 995, 1289, 1376, 1523, 1945, 2303
SmiMI CAYNNNNRTG 5 cut(s) 567, 1904, 2049, 2586, 2598
SmlI CTYRAG 1 cut(s) 2561
SmoI CTYRAG 1 cut(s) 2561
SpeI ACTAGT 1 cut(s) 74
SsiI CCGC 6 cut(s) 27, 114, 559, 1190, 2023, 2402
SspMI CTAG 2 cut(s) 75, 2510
StyD4I CCNGG 4 cut(s) 274, 342, 366, 499
StyI CCWWGG 4 cut(s) 1222, 1519, 2341, 2632
TaaI ACNGT 9 cut(s) 453, 888, 936, 1028, 1118, 1712, 1945, 2613, 2645
TaiI ACGT 2 cut(s) 1395, 2621
TaqI TCGA 2 cut(s) 2002, 2181
TatI WGTACW 2 cut(s) 71, 2217
TauI GCSGC 2 cut(s) 29, 562
TfiI GAWTC 2 cut(s) 170, 1084
Tru1I TTAA 6 cut(s) 63, 956, 1241, 1544, 1695, 1788
Tru9I TTAA 6 cut(s) 63, 956, 1241, 1544, 1695, 1788
TscAI CASTG 5 cut(s) 265, 456, 580, 1421, 2650
TseFI GTSAC 5 cut(s) 829, 867, 1307, 1445, 1892
TseI GCWGC 8 cut(s) 121, 645, 713, 716, 1492, 1668, 1680, 2068
Tsp45I GTSAC 5 cut(s) 829, 867, 1307, 1445, 1892
TspGWI ACGGA 5 cut(s) 579, 975, 1440, 1613, 1949
TspRI CASTG 5 cut(s) 265, 456, 580, 1421, 2650
Tth111I GACNNNGTC 1 cut(s) 1943
Van91I CCANNNNNTGG 1 cut(s) 1143
VpaK11BI GGWCC 7 cut(s) 317, 995, 1289, 1376, 1523, 1945, 2303
XagI CCTNNNNNAGG 1 cut(s) 1530
XapI RAATTY 7 cut(s) 190, 443, 608, 947, 1646, 1846, 2274
XceI RCATGY 2 cut(s) 1731, 2122
XmiI GTMKAC 1 cut(s) 1539
XmnI GAANNNNTTC 2 cut(s) 1183, 2394
XspI CTAG 2 cut(s) 75, 2510
ZrmI AGTACT 1 cut(s) 73
Zsp2I ATGCAT 1 cut(s) 1733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.