MD15G1062600.v1.1

Glycosyl hydrolase family 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006231
Physical Location & Seq
Forward (+)
4296309 .. 4301852
5544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1062600.v1.1.491

Sequence Viewer

Length: 3327 bp
ATGAGGAGGCTCATAGCTTGGTGCTTCAGAAACCGAGTCTCCGACAGCAGCAATCATCAGAAGCAGAAGCATCCAGAGGGATCCAACGAAGCTATGGACAAGAAGAACCAACAAACCAACAACAATGCCGCCGACAATGTGGAGTTCTTGAGGCAAAATGTTGCTGATTCAAGTAGCAGTCGCGGTCCAAATATCGTACTGAACCACGATTTCTCTGGAGGGCTGCATTCTTGGCACCACAATCACTGTAATGGCTTCGTAGTGGATTCAGCGGGAAGTTATGCGGTTGTCACGAATCGCCAACAATGCTGGCAGGGCTTGGAGCAAGACATTACTGGGAGGATTTCCCCTGGCAACACTTATTCAGTTTCAGCTCGTGTGGGAGTCTCAGGGACTCTTCAAGGATCTGCTGATGTCCTGGCAACTTTGAAGCTGGAAAGTCGAGGTTCAGCGACTAGCTATATGAGAATTGGAAGAAGTTCGGTGTCAAATGGGAAGTGGGAAAGCTTGGATGGAAAATTTTCGCTGTCGACAATGCCTGATCGGGTTGTGTTTTATTTGGAAGGGCCTCCTGCTGGAGTTGACCTTCATATCAAATCAGTTGTGATCTCTTGTTCTGAAGGCCAGTCTGAGAACCAAAATTTGGCCAATTCAAGTAGCAGTAATGCTACAAATATCATAATGAACCATGATTTCTCTGGAGGACTGCATTCTTGGCACCCGAATTGCTGCAATGGCTTTGTAGCTTCTGCTGATTCTGGTCACCCAGAAGTAAAAGCAGGCAATTATGCCGTTGTTACGAATCGTAAGGAAAGTTGGCAAGGTTTAGAGCAAGATATCACACGGAGAATTTCCCCAGGCTCCACATATTCGGTTTCAGCATGTGTTGGAGTCTGCGGATCTCTTCAGGGCTCCGCTGATGTCCTGGCAACTTTGAAGCTGGAATACAGAGGTTCAGCGACCAACCATTTGCAAGTTGGAAGATGTTCTGTATCAAAGGGAAGGTGGGGAAATTTGGATGGGAAGTTTTCTCTGTCGACAATGCCTGATAGGGTTGTATTTTATTTGGAAGGGCCTTCGCCTGGAGTCGACCTTCTAATAAAATCAGTTTTGATCTGTTCCTTGAGTCCAAATGAATGGCAGAGTGGAAGCACTGGGAATTTCAATGATGGAGAAGAGAATATAATCCTCAACCCAAACTTTGAGGATGCTTTGAATAATTGGTCTGGAAGAGGCTGCAAAATTGTTTTGCATGACTCAATGGGAGATGGGAAAATCGTTCCTCAATCTGGAAAGGTTTTTGCAGCTGCAACAGAACGCACACAGAGCTGGAATGGCATCCAGCAGGACATCACTGGAAGAGTGCAGCGAAAGCTAGCTTATGAGGCGACTGCTGTTGTTAGGATATTTGGCAACAATGTCACTACTGCTGTTGTCCGGGCTACCTTATGGGTCCAGTCACCAAACCAGCGCGAACAGTATATCGGCATTGCAAATGTGCAGGCAACAGACAAGGACTGGACACAGTTGCGGGGGAAGTTCCTTTTAAATGGCTCCCCGTCAAAAGTAGTAGTTTATCTTGAAGGTCCACTAGCGGGAACAGATATTCTTGTCAATAGTTTTGTTGTCAAGCATGCTGAGAAAGTTCCTCCTTCACCTCCACCGGTTATTGAGTTTTCAGCATTTGGAGTAAATATAATTGAGAACAGCAATTTAAGTAACGGCACAAACGGGTGGTTTCCGCTTGGTAATTGTACTTTGAGTGTCACAACTGGTTCACCACATATTCTCCCTCCAATGGCAAGAGAAAGCCTTGGACCTCATGAACCTTTAAGCGGTCGCTACATTCTAGTAACCAAGCGCACACAAACTTGGATGGGTCCTGCTCAGATGATTGGTGACAAATTGAAACTCTTTTTGACGTACCAAGTATCTGCTTGGGTTAGGATTGGTGCTGGAGCAACTGGTCCACAGAACGTCAATGTTGCACTCAGCGTGGACAACCAGTGGGTCAATGGAGCTCAAGCTGAGGTGAGTGACACAAGATGGCATGAAATCGGTGGTTCCTTCAGAGTTGAGAAGCAGCCATCGAAAGTAATGGTTTATATCCAAGGTCCTGCTGCAGGTGTAGACTTAATGGTTGCCGGTCTTCAGATATTCCCTGTTGACAGACCGGCGAGATTTAGACATCTGAAGAGGCAGACTGATAAGGTTCGTAAGTGCGATATTGTCCTGAAATTTTCAGGGTTGGACTCGAGCAGCATGCTCGGCACCTTTGTGAAAGTTAAACAAACGCAAAATAGTTTTCCAATTGGGACATGTATAAGCAGAACAAACATTGACAACGAAGATTACGTTGATTTCTTTATTAAAAACTTCAACTGGGCAGTGTTTGGAAATGAATTGAAGTGGTATTGGACTGAACCACAAAAAGGAAACTTCAACTACAAGGATGCTGATGAGATGGTGGACTTGTGCAAGAGTCACAACATAGAAATGCGAGGGCACTGTATTTTCTGGGAAGTCATTGACACAGTGCAGCAATGGATCCGCTCGTTGAGCCAGAGTGACTTGTCAACAGCTGTTCAGAATCGACTAACAGATCTTCTCACGCGCTACAAAGGGAAGTTCAGGCACTACGATGTCAACAATGAGATGCTGCACGGCTCATTCTATCAGGACAAATTGGGAAAAGATATCAGAGCCAACATGTTCAAGAATGCAAACCAGTTGGATCCATCTGCCACTCTATTTGTGAATGATTATCACGTCGAGGATGGATGCGATACTAGATCATCACCAGAAAAATACACTGATCAAATCCTTGATTTGCAGCAACAAGGTGCACCTGTTGGTGGCATTGGCATCCAAGGACATATTGATAGTCCAGTGGGTCCAATTGTTTGTACAGCTCTTGACAAATTAGGCATTCTCGGCCTTCCAATCTGGTTCACGGAGCTTGATGTGTCATCCAGTAACGAATATGTTAGAGCAGATGATTTGGAAGTGATGCTTCGCGAAGCATTTGCCAATCCAACAGTTGAAGGTGTAATGCTCTGGGGATTCTGGGAGCTGTTTATGAGTCGGGAAAATTCGCATTTAGTGAATGCAGAAGGGGATATCAACGAAGCAGGAAAACGTTTCTTGGAGCTCAAGCAAGAGTGGCTGTCTCATGCTCACGGCCACATTGACCAGCAGGGAGAGTTCGGGTTTAGGGGCTTCCCAGGAACATACAGTGTTGAAGTTTTTACTGCCTCGAAGAAGCCTGCAAAAACATTTGTCGTTGACAAGGGCGAGTCCCCAGTGGAGGTCTCCATTGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBM_4_9 Glyco_hydro_10

Protein Analysis

1109

Amino Acids

122.14

Weight (kDa)

6.31

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBM_4_9 PF02018 64 - 191 1.7e-19 Carbohydrate binding domain
CBM_4_9 PF02018 225 - 360 1.4e-19 Carbohydrate binding domain
CBM_4_9 PF02018 394 - 530 3e-19 Carbohydrate binding domain
CBM_4_9 PF02018 565 - 707 7.1e-17 Carbohydrate binding domain
Glyco_hydro_10 PF00331 765 - 1022 2.6e-47 Glycosyl hydrolase family 10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2114
Acc36I ACCTGC 1 cut(s) 2114
AccB1I GGYRCC 3 cut(s) 234, 717, 2270
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 2553
AccI GTMKAC 4 cut(s) 530, 1037, 1089, 2130
AccII CGCG 4 cut(s) 183, 1473, 2614, 3018
AclI AACGTT 1 cut(s) 3139
AclWI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
AcoI YGGCCR 2 cut(s) 645, 3181
AcsI RAATTY 7 cut(s) 518, 640, 849, 1012, 1159, 2237, 3091
AcuI CTGAAG 6 cut(s) 10, 639, 890, 2053, 2135, 2213
AfaI GTAC 4 cut(s) 198, 1756, 1925, 2908
AflIII ACRYGT 2 cut(s) 2318, 2709
AgeI ACCGGT 1 cut(s) 1663
AjiI CACGTC 1 cut(s) 2770
AjnI CCWGG 6 cut(s) 349, 417, 856, 924, 1081, 3223
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AleI CACNNNNGTG 1 cut(s) 2276
Alw21I GWGCWC 3 cut(s) 2023, 2848, 3153
Alw26I GTCTC 4 cut(s) 43, 391, 3174, 3316
Alw44I GTGCAC 1 cut(s) 2844
AlwI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
Ama87I CYCGRG 1 cut(s) 2254
AoxI GGCC 6 cut(s) 566, 622, 645, 1073, 2935, 3181
ApaLI GTGCAC 1 cut(s) 2844
ApoI RAATTY 7 cut(s) 518, 640, 849, 1012, 1159, 2237, 3091
ArsI GACNNNNNNTTYG 2 cut(s) 952, 984
AsiGI ACCGGT 1 cut(s) 1663
Asp700I GAANNNNTTC 3 cut(s) 478, 520, 985
AspLEI GCGC 3 cut(s) 1473, 1863, 2616
AsuC2I CCSGG 1 cut(s) 1439
AsuHPI GGTGA 7 cut(s) 755, 1452, 1647, 1770, 1910, 2044, 2790
AsuNHI GCTAGC 1 cut(s) 1375
AvaI CYCGRG 1 cut(s) 2254
AvaII GGWCC 8 cut(s) 185, 1453, 1586, 1817, 1880, 1967, 2114, 2894
BaeGI GKGCMC 2 cut(s) 2508, 2848
BalI TGGCCA 1 cut(s) 647
BamHI GGATCC 3 cut(s) 80, 2547, 2734
BanI GGYRCC 3 cut(s) 234, 717, 2270
BanII GRGCYC 3 cut(s) 914, 2023, 3153
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 2141
Bbv12I GWGCWC 3 cut(s) 2023, 2848, 3153
BbvCI CCTCAGC 1 cut(s) 2028
BceAI ACGGC 4 cut(s) 776, 1738, 2680, 3196
BcgI CGANNNNNNTGC 6 cut(s) 2245, 2248, 2279, 2282, 3008, 3042
BciT130I CCWGG 6 cut(s) 351, 419, 858, 926, 1083, 3225
BclI TGATCA 1 cut(s) 2815
BcnI CCSGG 1 cut(s) 1439
BcoDI GTCTC 4 cut(s) 43, 391, 3174, 3316
BfaI CTAG 5 cut(s) 456, 1376, 1592, 1850, 2790
BfmI CTRYAG 1 cut(s) 2121
BfuAI ACCTGC 1 cut(s) 2114
BglII AGATCT 1 cut(s) 2602
Bme1390I CCNGG 7 cut(s) 351, 419, 858, 926, 1083, 1439, 3225
Bme18I GGWCC 8 cut(s) 185, 1453, 1586, 1817, 1880, 1967, 2114, 2894
BmeT110I CYCGRG 1 cut(s) 2254
BmgBI CACGTC 1 cut(s) 2770
BmrFI CCNGG 7 cut(s) 351, 419, 858, 926, 1083, 1439, 3225
BmrI ACTGGG 4 cut(s) 345, 1164, 2392, 3296
BmsI GCATC 8 cut(s) 79, 1198, 1347, 2443, 2646, 2771, 2874, 3000
BmtI GCTAGC 1 cut(s) 1379
BmuI ACTGGG 4 cut(s) 345, 1164, 2392, 3296
BpiI GAAGAC 1 cut(s) 2141
BpmI CTGGAG 5 cut(s) 237, 597, 720, 1104, 1977
Bpu10I CCTNAGC 1 cut(s) 2028
BpuEI CTTGAG 4 cut(s) 169, 1144, 2007, 3137
BpuMI CCSGG 1 cut(s) 1439
BsaI GGTCTC 1 cut(s) 3316
BsaJI CCNNGG 6 cut(s) 349, 856, 1813, 2110, 2869, 3223
BsaWI WCCGGW 1 cut(s) 1663
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 1773, 1803
Bse118I RCCGGY 3 cut(s) 1663, 2144, 2173
Bse3DI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BseBI CCWGG 6 cut(s) 351, 419, 858, 926, 1083, 3225
BseDI CCNNGG 6 cut(s) 349, 856, 1813, 2110, 2869, 3223
BseMI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BseMII CTCAG 6 cut(s) 402, 621, 1629, 1901, 2005, 2019
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 2 cut(s) 2508, 2848
BsgI GTGCAG 4 cut(s) 1385, 1520, 2558, 2645
Bsh1236I CGCG 4 cut(s) 183, 1473, 2614, 3018
Bsh1285I CGRYCG 1 cut(s) 1840
BshFI GGCC 6 cut(s) 568, 624, 647, 1075, 2937, 3183
BshNI GGYRCC 3 cut(s) 234, 717, 2270
BshTI ACCGGT 1 cut(s) 1663
BsiEI CGRYCG 1 cut(s) 1840
BsiHKAI GWGCWC 3 cut(s) 2023, 2848, 3153
BsiHKCI CYCGRG 1 cut(s) 2254
BsiSI CCGG 4 cut(s) 1438, 1664, 2145, 2174
BslFI GGGAC 3 cut(s) 406, 2329, 3283
BsmAI GTCTC 4 cut(s) 43, 391, 3174, 3316
BsmFI GGGAC 3 cut(s) 406, 2329, 3283
BsmI GAATGC 5 cut(s) 226, 709, 2725, 2928, 3112
BsnI GGCC 6 cut(s) 568, 624, 647, 1075, 2937, 3183
Bso31I GGTCTC 1 cut(s) 3316
BsoBI CYCGRG 1 cut(s) 2254
Bsp1286I GDGCHC 5 cut(s) 914, 2023, 2508, 2848, 3153
Bsp1407I TGTACA 1 cut(s) 2906
Bsp68I TCGCGA 1 cut(s) 3018
BspANI GGCC 6 cut(s) 568, 624, 647, 1075, 2937, 3183
BspCNI CTCAG 6 cut(s) 401, 622, 1630, 1900, 2004, 2020
BspFNI CGCG 4 cut(s) 183, 1473, 2614, 3018
BspHI TCATGA 1 cut(s) 1822
BspMAI CTGCAG 1 cut(s) 2125
BspMI ACCTGC 1 cut(s) 2114
BspOI GCTAGC 1 cut(s) 1379
BspPI GGATC 8 cut(s) 75, 88, 412, 907, 2542, 2555, 2729, 2742
BspT107I GGYRCC 3 cut(s) 234, 717, 2270
BspTNI GGTCTC 1 cut(s) 3316
BsrBI CCGCTC 1 cut(s) 2553
BsrDI GCAATG 4 cut(s) 739, 1488, 2549, 3315
BsrFI RCCGGY 3 cut(s) 1663, 2144, 2173
BsrGI TGTACA 1 cut(s) 2906
BssAI RCCGGY 3 cut(s) 1663, 2144, 2173
BssECI CCNNGG 6 cut(s) 349, 856, 1813, 2110, 2869, 3223
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 3 cut(s) 1813, 2110, 2869
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 6 cut(s) 351, 419, 858, 926, 1083, 3225
Bst4CI ACNGT 7 cut(s) 248, 1479, 1527, 2510, 2536, 3040, 3236
Bst6I CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
BstAUI TGTACA 1 cut(s) 2906
BstC8I GCNNGC 7 cut(s) 311, 781, 1377, 1503, 1635, 2264, 3267
BstDEI CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
BstEII GGTNACC 1 cut(s) 761
BstENI CCTNNNNNAGG 1 cut(s) 2121
BstFNI CGCG 4 cut(s) 183, 1473, 2614, 3018
BstHHI GCGC 3 cut(s) 1473, 1863, 2616
BstMAI GTCTC 4 cut(s) 43, 391, 3174, 3316
BstMCI CGRYCG 1 cut(s) 1840
BstNI CCWGG 6 cut(s) 351, 419, 858, 926, 1083, 3225
BstNSI RCATGY 5 cut(s) 885, 1637, 2266, 2322, 2713
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 7 cut(s) 349, 417, 856, 924, 1081, 1437, 3223
BstSFI CTRYAG 1 cut(s) 2121
BstSLI GKGCMC 2 cut(s) 2508, 2848
BstUI CGCG 4 cut(s) 183, 1473, 2614, 3018
BstV2I GAAGAC 1 cut(s) 2141
BstX2I RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
BstXI CCANNNNNNTGG 1 cut(s) 1137
BstYI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
BsuRI GGCC 6 cut(s) 568, 624, 647, 1075, 2937, 3183
BtrI CACGTC 1 cut(s) 2770
BtsI GCAGTG 1 cut(s) 2394
BtuMI TCGCGA 1 cut(s) 3018
BveI ACCTGC 1 cut(s) 2114
Cac8I GCNNGC 7 cut(s) 311, 781, 1377, 1503, 1635, 2264, 3267
CciI TCATGA 1 cut(s) 1822
CfoI GCGC 3 cut(s) 1473, 1863, 2616
Cfr10I RCCGGY 3 cut(s) 1663, 2144, 2173
Csp6I GTAC 4 cut(s) 197, 1755, 1924, 2907
CspAI ACCGGT 1 cut(s) 1663
CspCI CAANNNNNGTGG 2 cut(s) 2734, 2769
CviQI GTAC 4 cut(s) 197, 1755, 1924, 2907
DdeI CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
DraI TTTAAA 1 cut(s) 1548
EaeI YGGCCR 2 cut(s) 645, 3181
Eam1104I CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
EarI CTCTTC 6 cut(s) 402, 909, 1170, 1225, 1354, 2189
Ecl136II GAGCTC 2 cut(s) 2021, 3151
Eco130I CCWWGG 3 cut(s) 1813, 2110, 2869
Eco24I GRGCYC 3 cut(s) 914, 2023, 3153
Eco31I GGTCTC 1 cut(s) 3316
Eco32I GATATC 3 cut(s) 838, 2698, 3121
Eco47I GGWCC 8 cut(s) 185, 1453, 1586, 1817, 1880, 1967, 2114, 2894
Eco53kI GAGCTC 2 cut(s) 2021, 3151
Eco57I CTGAAG 6 cut(s) 10, 639, 890, 2053, 2135, 2213
Eco88I CYCGRG 1 cut(s) 2254
Eco91I GGTNACC 1 cut(s) 761
EcoICRI GAGCTC 2 cut(s) 2021, 3151
EcoNI CCTNNNNNAGG 1 cut(s) 2121
EcoO109I RGGNCCY 4 cut(s) 566, 1073, 1880, 2114
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 6 cut(s) 349, 417, 856, 924, 1081, 3223
EcoRV GATATC 3 cut(s) 838, 2698, 3121
EcoT14I CCWWGG 3 cut(s) 1813, 2110, 2869
EcoT38I GRGCYC 3 cut(s) 914, 2023, 3153
ErhI CCWWGG 3 cut(s) 1813, 2110, 2869
FalI AAGNNNNNCTT 2 cut(s) 2997, 3029
FaqI GGGAC 3 cut(s) 406, 2329, 3283
FauI CCCGC 3 cut(s) 265, 1524, 1588
FbaI TGATCA 1 cut(s) 2815
FblI GTMKAC 4 cut(s) 530, 1037, 1089, 2130
FriOI GRGCYC 3 cut(s) 914, 2023, 3153
FspBI CTAG 5 cut(s) 456, 1376, 1592, 1850, 2790
GlaI GCGC 3 cut(s) 1472, 1862, 2615
GsuI CTGGAG 5 cut(s) 237, 597, 720, 1104, 1977
HaeIII GGCC 6 cut(s) 568, 624, 647, 1075, 2937, 3183
HapII CCGG 4 cut(s) 1438, 1664, 2145, 2174
HhaI GCGC 3 cut(s) 1473, 1863, 2616
Hin6I GCGC 3 cut(s) 1471, 1861, 2614
HinP1I GCGC 3 cut(s) 1471, 1861, 2614
HincII GTYRAC 8 cut(s) 531, 583, 1038, 1090, 2167, 2577, 2647, 3286
HindII GTYRAC 8 cut(s) 531, 583, 1038, 1090, 2167, 2577, 2647, 3286
HindIII AAGCTT 1 cut(s) 505
HpaII CCGG 4 cut(s) 1438, 1664, 2145, 2174
HphI GGTGA 7 cut(s) 755, 1452, 1647, 1770, 1910, 2044, 2790
Hpy99I CGWCG 1 cut(s) 2774
HpyCH4III ACNGT 7 cut(s) 248, 1479, 1527, 2510, 2536, 3040, 3236
HpyCH4IV ACGT 5 cut(s) 1922, 1977, 2355, 2769, 3139
HpyF3I CTNAG 6 cut(s) 388, 630, 1638, 1887, 1991, 2028
HpySE526I ACGT 5 cut(s) 1922, 1977, 2355, 2769, 3139
HspAI GCGC 3 cut(s) 1471, 1861, 2614
Ksp22I TGATCA 1 cut(s) 2815
LmnI GCTCC 9 cut(s) 322, 866, 917, 1559, 1958, 2018, 2956, 3070, 3148
LweI GCATC 8 cut(s) 79, 1198, 1347, 2443, 2646, 2771, 2874, 3000
MaeI CTAG 5 cut(s) 456, 1376, 1592, 1850, 2790
MaeII ACGT 5 cut(s) 1922, 1977, 2355, 2769, 3139
MbiI CCGCTC 1 cut(s) 2553
MfeI CAATTG 2 cut(s) 2310, 2898
MflI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
MhlI GDGCHC 5 cut(s) 914, 2023, 2508, 2848, 3153
MlsI TGGCCA 1 cut(s) 647
MluNI TGGCCA 1 cut(s) 647
MmeI TCCRAC 7 cut(s) 66, 108, 868, 958, 2229, 2712, 3059
Mox20I TGGCCA 1 cut(s) 647
MroXI GAANNNNTTC 3 cut(s) 478, 520, 985
MscI TGGCCA 1 cut(s) 647
MseI TTAA 6 cut(s) 1547, 1715, 1832, 2135, 2286, 2370
MslI CAYNNNNRTG 4 cut(s) 249, 2276, 2495, 2640
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 4 cut(s) 272, 917, 1307, 2582
MspI CCGG 4 cut(s) 1438, 1664, 2145, 2174
MspR9I CCNGG 7 cut(s) 351, 419, 858, 926, 1083, 1439, 3225
MunI CAATTG 2 cut(s) 2310, 2898
Mva1269I GAATGC 5 cut(s) 226, 709, 2725, 2928, 3112
MvaI CCWGG 6 cut(s) 351, 419, 858, 926, 1083, 3225
MvnI CGCG 4 cut(s) 183, 1473, 2614, 3018
NciI CCSGG 1 cut(s) 1439
NheI GCTAGC 1 cut(s) 1375
NmeAIII GCCGAG 2 cut(s) 2247, 2913
NmuCI GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
NruI TCGCGA 1 cut(s) 3018
NspI RCATGY 5 cut(s) 885, 1637, 2266, 2322, 2713
OliI CACNNNNGTG 1 cut(s) 2276
PaeI GCATGC 2 cut(s) 1637, 2266
PaeR7I CTCGAG 1 cut(s) 2254
PagI TCATGA 1 cut(s) 1822
PaqCI CACCTGC 1 cut(s) 2114
PciI ACATGT 2 cut(s) 2318, 2709
PcsI WCGNNNNNNNCGW 1 cut(s) 2352
PctI GAATGC 5 cut(s) 226, 709, 2725, 2928, 3112
PdmI GAANNNNTTC 3 cut(s) 478, 520, 985
PfeI GAWTC 7 cut(s) 167, 266, 295, 755, 802, 2590, 3063
PflMI CCANNNNNTGG 1 cut(s) 643
PinAI ACCGGT 1 cut(s) 1663
PpuMI RGGWCCY 2 cut(s) 1880, 2114
PscI ACATGT 2 cut(s) 2318, 2709
Psp124BI GAGCTC 2 cut(s) 2023, 3153
Psp1406I AACGTT 1 cut(s) 3139
Psp5II RGGWCCY 2 cut(s) 1880, 2114
Psp6I CCWGG 6 cut(s) 349, 417, 856, 924, 1081, 3223
PspEI GGTNACC 1 cut(s) 761
PspGI CCWGG 6 cut(s) 349, 417, 856, 924, 1081, 3223
PspPPI RGGWCCY 2 cut(s) 1880, 2114
PspXI VCTCGAGB 1 cut(s) 2254
PstI CTGCAG 1 cut(s) 2125
PsuI RGATCY 6 cut(s) 80, 404, 899, 2547, 2602, 2734
PvuII CAGCTG 2 cut(s) 1307, 2582
RruI TCGCGA 1 cut(s) 3018
RsaI GTAC 4 cut(s) 198, 1756, 1925, 2908
RsaNI GTAC 4 cut(s) 197, 1755, 1924, 2907
RseI CAYNNNNRTG 4 cut(s) 249, 2276, 2495, 2640
SacI GAGCTC 2 cut(s) 2023, 3153
SalI GTCGAC 3 cut(s) 529, 1036, 1088
SaqAI TTAA 6 cut(s) 1547, 1715, 1832, 2135, 2286, 2370
ScrFI CCNGG 7 cut(s) 351, 419, 858, 926, 1083, 1439, 3225
SduI GDGCHC 5 cut(s) 914, 2023, 2508, 2848, 3153
SfaNI GCATC 8 cut(s) 79, 1198, 1347, 2443, 2646, 2771, 2874, 3000
SfcI CTRYAG 1 cut(s) 2121
Sfr274I CTCGAG 1 cut(s) 2254
SinI GGWCC 8 cut(s) 185, 1453, 1586, 1817, 1880, 1967, 2114, 2894
SlaI CTCGAG 1 cut(s) 2254
SmiMI CAYNNNNRTG 4 cut(s) 249, 2276, 2495, 2640
SmlI CTYRAG 5 cut(s) 148, 1123, 2022, 2254, 3152
SmoI CTYRAG 5 cut(s) 148, 1123, 2022, 2254, 3152
SphI GCATGC 2 cut(s) 1637, 2266
SspMI CTAG 5 cut(s) 456, 1376, 1592, 1850, 2790
SstI GAGCTC 2 cut(s) 2023, 3153
StyD4I CCNGG 7 cut(s) 349, 417, 856, 924, 1081, 1437, 3223
StyI CCWWGG 3 cut(s) 1813, 2110, 2869
TaaI ACNGT 7 cut(s) 248, 1479, 1527, 2510, 2536, 3040, 3236
TaiI ACGT 5 cut(s) 1925, 1980, 2358, 2772, 3142
TaqI TCGA 9 cut(s) 442, 530, 1037, 1089, 2090, 2255, 2593, 2772, 3257
TatI WGTACW 2 cut(s) 1754, 2906
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 7 cut(s) 167, 266, 295, 755, 802, 2590, 3063
Tru1I TTAA 6 cut(s) 1547, 1715, 1832, 2135, 2286, 2370
Tru9I TTAA 6 cut(s) 1547, 1715, 1832, 2135, 2286, 2370
TseFI GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
Tsp45I GTSAC 9 cut(s) 289, 761, 1420, 1458, 1765, 1898, 2036, 2483, 2567
TspDTI ATGAA 6 cut(s) 578, 698, 1149, 1839, 2067, 2415
TspGWI ACGGA 2 cut(s) 859, 2969
Van91I CCANNNNNTGG 1 cut(s) 643
VneI GTGCAC 1 cut(s) 2844
VpaK11BI GGWCC 8 cut(s) 185, 1453, 1586, 1817, 1880, 1967, 2114, 2894
XagI CCTNNNNNAGG 1 cut(s) 2121
XapI RAATTY 7 cut(s) 518, 640, 849, 1012, 1159, 2237, 3091
XceI RCATGY 5 cut(s) 885, 1637, 2266, 2322, 2713
XcmI CCANNNNNNNNNTGG 5 cut(s) 91, 212, 695, 974, 2012
XhoI CTCGAG 1 cut(s) 2254
XmiI GTMKAC 4 cut(s) 530, 1037, 1089, 2130
XmnI GAANNNNTTC 3 cut(s) 478, 520, 985
XspI CTAG 5 cut(s) 456, 1376, 1592, 1850, 2790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.