MD08G1076700.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007467
Physical Location & Seq
Forward (+)
6287923 .. 6291101
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1076700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGAGCGGCGGCGGCGGCGATGACCGTGATCGGAGTAGGAATAGGAATGGACCGTGGACTCCGCCAGGCAGACCGTACGAGGAAAAAGCGGAAGACATGAAGATTTGGGGGATTCTGGTGTTTGGTCTAATTGGCGCCACCGCCACCACTCTCGCCGTTTCTCAGCTTCGTAGGACTGTTGATTGGGTCTATACTCAGTTGTTCAGATCGCCATCAACGGGGAGAGGAAGTGGCAGTTCATTCCGGACAGCTTTTCAGGAGGAAGCATGGAAAAGATACAACCGTCGCATGCGAGAGGAGTATGAAGAAGAAATGGAGAGAGTGGAACGTATTAGGCGTATGCAAAGCGTGTTCAATAGAGAGAGGAGCAAATACAAAAGAACCTACGAGAGCTGGAGGGAAAACGATCAGGGTGGATATCATCAACAGTTCCAGCGAGAGGATTGGTATTGGAAGACTGATACATCCTTTAAAGACCGGAGGAGTAGCTACAGGGAAACTCCCAGAGAAAATGCCAACTATTTGTTATCACATCACTACTCAGTTTTAGGCCTTGACAGGTTTAGAAAGGCACCTTACACAGACACAGAGATTAAGACAGCATTTAGGACCAAGGCGAAGCAGTTTCACCCAGATCAGAACCAAAACAATAAAGTGGCTGCTGAAGCAAAGTTTAAAGAGGTAATGACATCGTATGAAGCAATAAAGAAAGAGAGGGGGAACACGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

245

Amino Acids

29.08

Weight (kDa)

9.77

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 179 - 238 1.9e-12 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 134, 571
AccBSI CCGCTC 1 cut(s) 6
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 7 cut(s) 6, 9, 12, 15, 62, 89, 141
AcuI CTGAAG 1 cut(s) 684
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 218, 439
AgsI TTSAA 1 cut(s) 355
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 4 cut(s) 166, 251, 393, 489
AluI AGCT 4 cut(s) 166, 251, 393, 489
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 50, 609
AsuHPI GGTGA 1 cut(s) 620
AvaII GGWCC 2 cut(s) 50, 609
BaeI ACNNNNGTAYC 2 cut(s) 268, 301
BanI GGYRCC 2 cut(s) 134, 571
BauI CACGAG 1 cut(s) 724
BbsI GAAGAC 2 cut(s) 99, 461
BbvI GCAGC 1 cut(s) 646
BccI CCATC 1 cut(s) 220
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 490
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 5 cut(s) 7, 10, 13, 16, 660
BlsI GCNGC 5 cut(s) 8, 11, 14, 17, 661
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 2 cut(s) 50, 609
BmgT120I GGNCC 2 cut(s) 50, 609
BmiI GGNNCC 2 cut(s) 136, 573
BmrFI CCNGG 1 cut(s) 66
BpiI GAAGAC 2 cut(s) 99, 461
BpmI CTGGAG 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 53, 612
BsaWI WCCGGW 2 cut(s) 243, 477
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 439
Bse8I GATNNNNATC 1 cut(s) 211
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 53, 612
BseGI GGATG 1 cut(s) 464
BseJI GATNNNNATC 1 cut(s) 211
BseLI CCNNNNNNNGG 2 cut(s) 218, 439
BseMII CTCAG 3 cut(s) 176, 209, 555
BseRI GAGGAG 3 cut(s) 311, 379, 496
BseXI GCAGC 1 cut(s) 646
BshFI GGCC 1 cut(s) 552
BshNI GGYRCC 2 cut(s) 134, 571
BsiSI CCGG 2 cut(s) 244, 478
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 218, 439
BsnI GGCC 1 cut(s) 552
Bsp13I TCCGGA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 28, 206, 406, 634
BspACI CCGC 7 cut(s) 6, 9, 12, 15, 62, 89, 141
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 3 cut(s) 175, 208, 554
BspEI TCCGGA 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 136, 573
BspT107I GGYRCC 2 cut(s) 134, 571
BsrBI CCGCTC 1 cut(s) 6
BssECI CCNNGG 2 cut(s) 53, 612
BssMI GATC 4 cut(s) 28, 206, 406, 634
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 724
BssT1I CCWWGG 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 6 cut(s) 26, 54, 75, 178, 284, 429
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 290
BstDEI CTNAG 3 cut(s) 162, 195, 541
BstDSI CCRYGG 1 cut(s) 53
BstF5I GGATG 1 cut(s) 464
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 4 cut(s) 31, 209, 409, 637
BstMBI GATC 4 cut(s) 28, 206, 406, 634
BstMWI GCNNNNNNNGC 3 cut(s) 12, 15, 665
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 490
BstV1I GCAGC 1 cut(s) 646
BstV2I GAAGAC 2 cut(s) 99, 461
BsuRI GGCC 1 cut(s) 552
BtgI CCRYGG 1 cut(s) 53
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 290
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 50, 609
Csp6I GTAC 1 cut(s) 76
CviAII CATG 3 cut(s) 97, 267, 289
CviJI RGCY 6 cut(s) 166, 251, 393, 489, 552, 659
CviKI_1 RGCY 6 cut(s) 166, 251, 393, 489, 552, 659
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 3 cut(s) 162, 195, 541
DinI GGCGCC 1 cut(s) 136
DpnI GATC 4 cut(s) 30, 208, 408, 636
DpnII GATC 4 cut(s) 28, 206, 406, 634
DraI TTTAAA 2 cut(s) 472, 676
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 612
Eco147I AGGCCT 1 cut(s) 552
Eco32I GATATC 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 50, 609
Eco57I CTGAAG 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 64
EcoRV GATATC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 612
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 3 cut(s) 100, 270, 292
FaiI YATR 7 cut(s) 98, 192, 268, 290, 303, 341, 696
FatI CATG 3 cut(s) 96, 266, 288
Fnu4HI GCNGC 5 cut(s) 7, 10, 13, 16, 660
FokI GGATG 1 cut(s) 451
Fsp4HI GCNGC 5 cut(s) 7, 10, 13, 16, 660
GlaI GCGC 1 cut(s) 136
GluI GCNGC 5 cut(s) 7, 10, 13, 16, 660
GsuI CTGGAG 1 cut(s) 415
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 1 cut(s) 552
HapII CCGG 2 cut(s) 244, 478
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 3 cut(s) 100, 270, 292
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 2 cut(s) 58, 112
HpaII CCGG 2 cut(s) 244, 478
HphI GGTGA 1 cut(s) 620
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 3 cut(s) 33, 206, 639
Hpy188III TCNNGA 2 cut(s) 244, 257
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 1 cut(s) 288
HpyCH4III ACNGT 6 cut(s) 26, 54, 75, 178, 284, 429
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 1 cut(s) 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 15, 665
HpyF3I CTNAG 3 cut(s) 162, 195, 541
HpySE526I ACGT 1 cut(s) 328
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 3 cut(s) 100, 270, 292
HspAI GCGC 1 cut(s) 135
KasI GGCGCC 1 cut(s) 134
Kpn2I TCCGGA 1 cut(s) 243
Kzo9I GATC 4 cut(s) 28, 206, 406, 634
LmnI GCTCC 1 cut(s) 366
Lsp1109I GCAGC 1 cut(s) 646
MaeII ACGT 1 cut(s) 328
MalI GATC 4 cut(s) 30, 208, 408, 636
MbiI CCGCTC 1 cut(s) 6
MboI GATC 4 cut(s) 28, 206, 406, 634
MboII GAAGA 5 cut(s) 104, 112, 317, 320, 466
MluCI AATT 1 cut(s) 129
Mly113I GGCGCC 1 cut(s) 135
MlyI GAGTC 1 cut(s) 52
MroI TCCGGA 1 cut(s) 243
MseI TTAA 3 cut(s) 471, 594, 675
MspI CCGG 2 cut(s) 244, 478
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 12, 15, 665
NarI GGCGCC 1 cut(s) 135
NdeII GATC 4 cut(s) 28, 206, 406, 634
NlaIII CATG 3 cut(s) 100, 270, 292
NlaIV GGNNCC 2 cut(s) 136, 573
NspI RCATGY 1 cut(s) 292
PaeI GCATGC 1 cut(s) 292
PceI AGGCCT 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PfeI GAWTC 1 cut(s) 112
Pfl23II CGTACG 1 cut(s) 75
PkrI GCNGC 5 cut(s) 8, 11, 14, 17, 661
PleI GAGTC 1 cut(s) 52
PluTI GGCGCC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 136, 573
PspPI GGNCC 2 cut(s) 50, 609
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SaqAI TTAA 3 cut(s) 471, 594, 675
SatI GCNGC 5 cut(s) 7, 10, 13, 16, 660
Sau3AI GATC 4 cut(s) 28, 206, 406, 634
Sau96I GGNCC 2 cut(s) 50, 609
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 66
SetI ASST 9 cut(s) 168, 253, 331, 386, 395, 491, 563, 577, 684
SfcI CTRYAG 1 cut(s) 490
SfoI GGCGCC 1 cut(s) 136
SinI GGWCC 2 cut(s) 50, 609
SphI GCATGC 1 cut(s) 292
Sse9I AATT 1 cut(s) 129
SseBI AGGCCT 1 cut(s) 552
SsiI CCGC 7 cut(s) 6, 9, 12, 15, 62, 89, 141
SspDI GGCGCC 1 cut(s) 134
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 6 cut(s) 26, 54, 75, 178, 284, 429
TaiI ACGT 1 cut(s) 331
TasI AATT 1 cut(s) 129
TauI GCSGC 4 cut(s) 9, 12, 15, 18
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 3 cut(s) 471, 594, 675
Tru9I TTAA 3 cut(s) 471, 594, 675
TseI GCWGC 1 cut(s) 659
TspDTI ATGAA 4 cut(s) 113, 228, 318, 711
VpaK11BI GGWCC 2 cut(s) 50, 609
XceI RCATGY 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.