MD15G1064000.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007467
Physical Location & Seq
Forward (+)
4432006 .. 4434969
2964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1064000.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGGCGGCGGCGACGACGACGGTGATCGGAGTAGGAATAGGAAAGGGCCGTGGACGCTGCCGGGAAGACCGTATGAGGCAAAAGAGGAAGACATAAAGATTTGGGGGATTCTGGTGTTTGGTCTAATTGGCGCCACCGCCACCACTCTCGCCGTTTCTCAGCTCCGTAGGACTGTTGATTGGGTGTATACACAGTTGTTCAGATCACCGTCGACAGGGAGAGGAAGTGGCAATTCATTCCGGACAGCTTTTCAGGAGGAAGCATGGAAAAGATATAACCGTCGGATGCAAGAGGAGTATGAAGAAGAAATGGAGAGAGTGGAACGTATTAGGCGTATGCAAAATGTTTTCAATAGAGAGAGGAACAAACACAAAAGGACCTATGAGAGCTGGAGGGAAAACAGTCAAGGTGGATATCATCAGCAATTCCAGCGAGAGGATTGGTATTGGAAGACTGATACATCCTTTAAAGACAGGAGGAGTAACTACAGGGAAACTCCTAGAGAAAATGCCAACTATTTGTTATCACATCACTACTCAGTTTTAGGCCTTGACAGGTTTAGAAAGGCACCTTACTCGGATGCGGAGATTAAGACAGCATTTAGGACCAAGGCGAAGCAGTTTCACCCCGACCAGAACCAAAACAACAAAGGGGCTGCTGAAGCAAAGTTTAAAGAGGTAATGACGTCGTATGAGGCAATAAAGAAAGAGAGGGGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

241

Amino Acids

28.6

Weight (kDa)

9.71

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 178 - 237 1.2e-12 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 689
AccB1I GGYRCC 2 cut(s) 131, 568
AccI GTMKAC 2 cut(s) 188, 212
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 4 cut(s) 6, 9, 138, 584
AcuI CTGAAG 1 cut(s) 681
AcyI GRCGYC 2 cut(s) 132, 686
AfiI CCNNNNNNNGG 2 cut(s) 215, 436
AgsI TTSAA 1 cut(s) 352
AluBI AGCT 3 cut(s) 163, 248, 390
AluI AGCT 3 cut(s) 163, 248, 390
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 2 cut(s) 47, 547
ApeKI GCWGC 2 cut(s) 58, 656
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 3 cut(s) 47, 378, 606
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 3 cut(s) 35, 198, 617
AvaII GGWCC 2 cut(s) 378, 606
BanI GGYRCC 2 cut(s) 131, 568
BbsI GAAGAC 3 cut(s) 73, 96, 458
BbvI GCAGC 2 cut(s) 45, 643
BceAI ACGGC 2 cut(s) 34, 137
BcgI CGANNNNNNTGC 2 cut(s) 558, 592
BcnI CCSGG 1 cut(s) 63
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 487
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 4 cut(s) 7, 10, 59, 657
BlsI GCNGC 4 cut(s) 8, 11, 60, 658
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 378, 606
BmgT120I GGNCC 3 cut(s) 47, 378, 606
BmiI GGNNCC 2 cut(s) 133, 570
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 2 cut(s) 276, 571
BpiI GAAGAC 3 cut(s) 73, 96, 458
BpmI CTGGAG 1 cut(s) 412
BpuMI CCSGG 1 cut(s) 63
BsaHI GRCGYC 2 cut(s) 132, 686
BsaJI CCNNGG 2 cut(s) 50, 609
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 436
BseAI TCCGGA 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 50, 609
BseGI GGATG 3 cut(s) 291, 461, 586
BseLI CCNNNNNNNGG 2 cut(s) 215, 436
BseMII CTCAG 2 cut(s) 173, 552
BseRI GAGGAG 2 cut(s) 308, 493
BseXI GCAGC 2 cut(s) 45, 643
BshFI GGCC 2 cut(s) 49, 549
BshNI GGYRCC 2 cut(s) 131, 568
BsiSI CCGG 2 cut(s) 62, 241
BslI CCNNNNNNNGG 2 cut(s) 215, 436
BsnI GGCC 2 cut(s) 49, 549
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 2 cut(s) 25, 203
BspACI CCGC 4 cut(s) 6, 9, 138, 584
BspANI GGCC 2 cut(s) 49, 549
BspCNI CTCAG 2 cut(s) 172, 551
BspEI TCCGGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 133, 570
BspT107I GGYRCC 2 cut(s) 131, 568
BssECI CCNNGG 2 cut(s) 50, 609
BssMI GATC 2 cut(s) 25, 203
BssNAI GTATAC 1 cut(s) 189
BssNI GRCGYC 2 cut(s) 132, 686
BssT1I CCWWGG 1 cut(s) 609
Bst1107I GTATAC 1 cut(s) 189
Bst4CI ACNGT 7 cut(s) 23, 72, 175, 195, 210, 281, 404
BstACI GRCGYC 2 cut(s) 132, 686
BstDEI CTNAG 2 cut(s) 159, 538
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 291, 461, 586
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 28, 206
BstMBI GATC 2 cut(s) 25, 203
BstMWI GCNNNNNNNGC 3 cut(s) 55, 430, 662
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 45, 643
BstV2I GAAGAC 3 cut(s) 73, 96, 458
BstZ17I GTATAC 1 cut(s) 189
BsuRI GGCC 2 cut(s) 49, 549
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 291, 461, 586
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 3 cut(s) 47, 378, 606
CseI GACGC 1 cut(s) 64
CviAII CATG 1 cut(s) 264
CviJI RGCY 6 cut(s) 49, 163, 248, 390, 549, 656
CviKI_1 RGCY 6 cut(s) 49, 163, 248, 390, 549, 656
DdeI CTNAG 2 cut(s) 159, 538
DinI GGCGCC 1 cut(s) 133
DpnI GATC 2 cut(s) 27, 205
DpnII GATC 2 cut(s) 25, 203
DraI TTTAAA 2 cut(s) 469, 673
Eco130I CCWWGG 1 cut(s) 609
Eco147I AGGCCT 1 cut(s) 549
Eco32I GATATC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 378, 606
Eco57I CTGAAG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 378
EcoRV GATATC 1 cut(s) 416
EcoT14I CCWWGG 1 cut(s) 609
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 1 cut(s) 267
FaiI YATR 9 cut(s) 75, 95, 189, 265, 276, 300, 338, 384, 693
FatI CATG 1 cut(s) 263
FblI GTMKAC 2 cut(s) 188, 212
Fnu4HI GCNGC 4 cut(s) 7, 10, 59, 657
FokI GGATG 3 cut(s) 298, 448, 593
Fsp4HI GCNGC 4 cut(s) 7, 10, 59, 657
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 133
GluI GCNGC 4 cut(s) 7, 10, 59, 657
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 2 cut(s) 49, 549
HapII CCGG 2 cut(s) 62, 241
HgaI GACGC 1 cut(s) 64
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 2 cut(s) 132, 686
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 1 cut(s) 109
HpaII CCGG 2 cut(s) 62, 241
HphI GGTGA 3 cut(s) 35, 198, 617
Hpy166II GTNNAC 3 cut(s) 54, 189, 213
Hpy188I TCNGA 4 cut(s) 30, 203, 285, 580
Hpy188III TCNNGA 2 cut(s) 241, 254
Hpy8I GTNNAC 3 cut(s) 54, 189, 213
Hpy99I CGWCG 6 cut(s) 17, 20, 23, 214, 285, 691
HpyCH4III ACNGT 7 cut(s) 23, 72, 175, 195, 210, 281, 404
HpyCH4IV ACGT 2 cut(s) 325, 686
HpyCH4V TGCA 2 cut(s) 289, 340
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 430, 662
HpyF3I CTNAG 2 cut(s) 159, 538
HpySE526I ACGT 2 cut(s) 325, 686
Hsp92I GRCGYC 2 cut(s) 132, 686
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 1 cut(s) 132
KasI GGCGCC 1 cut(s) 131
Kpn2I TCCGGA 1 cut(s) 240
Kzo9I GATC 2 cut(s) 25, 203
LmnI GCTCC 1 cut(s) 168
Lsp1109I GCAGC 2 cut(s) 45, 643
LweI GCATC 2 cut(s) 276, 571
MaeI CTAG 1 cut(s) 501
MaeII ACGT 2 cut(s) 325, 686
MaeIII GTNAC 1 cut(s) 482
MalI GATC 2 cut(s) 27, 205
MboI GATC 2 cut(s) 25, 203
MboII GAAGA 5 cut(s) 78, 101, 314, 317, 463
MluCI AATT 3 cut(s) 126, 232, 425
Mly113I GGCGCC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 263
MroI TCCGGA 1 cut(s) 240
MseI TTAA 3 cut(s) 468, 591, 672
MspI CCGG 2 cut(s) 62, 241
MspR9I CCNGG 1 cut(s) 63
MwoI GCNNNNNNNGC 3 cut(s) 55, 430, 662
NarI GGCGCC 1 cut(s) 132
NciI CCSGG 1 cut(s) 63
NdeII GATC 2 cut(s) 25, 203
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 2 cut(s) 133, 570
PceI AGGCCT 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 4 cut(s) 8, 11, 60, 658
PluTI GGCGCC 1 cut(s) 135
PpuMI RGGWCCY 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 133, 570
PspPI GGNCC 3 cut(s) 47, 378, 606
PspPPI RGGWCCY 1 cut(s) 378
SalI GTCGAC 1 cut(s) 211
SaqAI TTAA 3 cut(s) 468, 591, 672
SatI GCNGC 4 cut(s) 7, 10, 59, 657
Sau3AI GATC 2 cut(s) 25, 203
Sau96I GGNCC 3 cut(s) 47, 378, 606
ScrFI CCNGG 1 cut(s) 63
SfaNI GCATC 2 cut(s) 276, 571
SfcI CTRYAG 1 cut(s) 487
SfoI GGCGCC 1 cut(s) 133
SinI GGWCC 2 cut(s) 378, 606
Sse9I AATT 3 cut(s) 126, 232, 425
SseBI AGGCCT 1 cut(s) 549
SsiI CCGC 4 cut(s) 6, 9, 138, 584
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 1 cut(s) 501
StuI AGGCCT 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 7 cut(s) 23, 72, 175, 195, 210, 281, 404
TaiI ACGT 2 cut(s) 328, 689
TaqI TCGA 1 cut(s) 212
TasI AATT 3 cut(s) 126, 232, 425
TauI GCSGC 2 cut(s) 9, 12
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 3 cut(s) 468, 591, 672
Tru9I TTAA 3 cut(s) 468, 591, 672
TseI GCWGC 2 cut(s) 58, 656
TspDTI ATGAA 2 cut(s) 225, 315
TspGWI ACGGA 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 378, 606
XmiI GTMKAC 2 cut(s) 188, 212
XspI CTAG 1 cut(s) 501
ZraI GACGTC 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.