MD08G1076900.v1.1

xyloglucan glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014524
Physical Location & Seq
Forward (+)
6330889 .. 6334506
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1076900.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGCGCCCTCTTTCAGCTGGTGGAATAATACTAAAGACACCCACCGCGGCACACCCGTCGTCATCAAGATGGAGAACCCCAACTGGTCCATGGTCGAGCTCGAAGGCCCCTCCGACGAGGACTTCCTCATCTCCGAGTCGCCGGGTCGTGTCCGTGGCAAAAACGCCAAGCAGTTCACTTGGGTCCTCCTCCTCAAAGCCCACAGGGCCGCCGGCTGCCTCACCTCCATCGGCTCCGCAATGCTGGGCCTCGCATCGGCAATCCGCCGTCGCGTCGCCTCCGGCCGGATGGACACCGACGCCGACGTCGAGCCCCACGGCGTCAGGGAAGTCGAGAACCCGGCCGTCCGCAGCCGGTTCTATTTCTGTATAAAGATGTTCTTGTGTCTGTCGGTTCTTCTTCTGGGTTTTGAGGTGGCGGCGTATTTCAAAGGCTGGCACTTTGGGCCGGCGCATCTGCAGCTGGAGTACTTATGGGCGTCGCCATTTGGGGTTAAGGGCGCGTTTGATTGGGTTTATTCGAAATGGGTTTTGTTCCGGGTGCAGTATTTAGCTCCGCCGCTGCAGTTCCTCGCCAGTTCCTGCATTGTTCTTTTCATGATTCAGAGCTTGGATAGGCTAATCCTCTGTCTCGGTTGCTTCTGGATCCGGTTCAAGAAGATCAAACCGGTCCCGAAAGAAGGGTCTTTCGACCCCGAAACAGGGGAAACCGGTTACTTCCCCATGGTTCTTGTTCAGATCCCCATGTGCAATGAAAAGGAGGTTTATCAGACGTCAATTGCTGCAGTGTGCAATCTGGACTGGCCGAAATCGAAGATGTTGATTCAAATTCTCGACGATTCGGATGATCCAACGACGCAGTTTCTGATCAAGGAGGAAGTCCATAAGTGGCAGCAGGAGGGAGCCAACATTGTGTACCGGCATCGCGTGATTAGAGATGGGTACAAGGCTGGTAATCTCAAGTCTGCAATGAATTGTAGCTATGTCAAGGACTATGAATTCGTCGCAATTTTCGACGCAGATTTTCAGCCCACCCCTGATTTCCTCAAGAGAACAGTCCCTCACTTCATGGGCAATGATGAACTAGGGCTGGTTCAGGCTAGATGGTCCTTTGTGAACAGGGATGAGAACCTGCTCACAAGGCTGCAGAACATTAACTTGTCATTCCACTTTGAGGTTGAGCAACAAGTGAACAGCGTGTTCATTAATTTCTTCGGTTTCAATGGGACAGCTGGTGTGTGGAGGATAAAGGCGTTGGAGGAAGCTGGTGGGTGGTTAGAGAGGACCACTGTCGAGGACATGGATATTGCTGTTCGTGCCCATCTCCACGGATGGAAATTCATTTTCCTCAATGACGTCGAGTGCCAGTGTGAATTACCGGAATCTTATGAAGCTTATCGGAAGCAACAACACCGATGGCACTCGGGGCCTATGCAATTATTTCGCCTCTGTTTGCCAGCTATTATCAAATCCAAGATCAGCATTGGCAAGAAATTCAATTTAATCTTTCTCTTCTTTCTGCTTCGGAAACTTATATTACCCTTCTATTCGTTTACCCTTTTTTGCATAATTCTCCCCATGACAATGTTCATCCCAGAGGCCGAGCTACCAGCGTGGGTTGTATGTTACATCCCCGCCACCATGTCGTTTCTCAACATTCTCCCAGCCCCAAAAGCCTTCCCTTTCATTGTTCCTTACCTTCTCTTTGAGAACACCATGTCAGTGACCAAGTTCAACGCTATGATCTCCGGCCTGTTCCAGCTTGGCAGCGCATACGAGTGGGTTGTCACTAAGAAATCTGGCCGTTCCTCAGAGGGCGATCTCACCTCCTTAGTTGAAAAGCCAAAGCATCAAAGGGGGGTCTCAGTGCCGGACCTTGATGAAATGAAGGAAGAAATTAGACAGCAGGAGCAAAAGGTTTCCCGGAAGAAGAAGCACAACAGAATATACACAAAGGAGTTGGCATTGGCTTTCCTTCTCCTAACTGCCGCGGCGAGGAGCCTTCTGTCTGCTCAGGGTATCCACTTCTACTTCTTGTTGTTTCAGGGAGTATCGTTCCTGCTGGTCGGTCTAGACCTAATCGGTGAGCAGGTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

700

Amino Acids

80.18

Weight (kDa)

8.24

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 241 - 474 3.9e-13 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 246 - 392 4.2e-10 Glycosyl transferase family 2
Glyco_transf_21 PF13506 330 - 474 6.1e-08 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 335 - 543 4.7e-21 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G07960 AT4G07960
fragaria_vesca FvH4_2g32570
malus_domestica MD08G1076900.v1.1 MD15G1064400.v1.1
prunus_persica Prupe.1G418400_v2.0.a1
pyrus_communis pycom08g06370 pycom15g06110
rosa_chinensis RchiOBHm_Chr6g0310111
rosa_laevigata RLG00000010510
rosa_multiflora Rmu_sc0000294.1_g000027
rosa_roxburghii Rroxscaffold_7G00158860
rosa_rugosa Rorug06G0377600
rosa_samantha Rh6BG500800 Rh6CG505900 Rh6DG491800
rosa_wichuraiana Rw6G042760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2080
AasI GACNNNNNNGTC 1 cut(s) 305
AatII GACGTC 3 cut(s) 309, 776, 1359
Acc36I ACCTGC 2 cut(s) 1140, 2080
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 5 cut(s) 48, 273, 503, 927, 1991
AclWI GGATC 4 cut(s) 640, 653, 733, 842
AcoI YGGCCR 4 cut(s) 283, 342, 803, 1801
AcsI RAATTY 4 cut(s) 828, 998, 1337, 1493
AcyI GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
AfaI GTAC 3 cut(s) 470, 917, 944
AfiI CCNNNNNNNGG 7 cut(s) 256, 285, 680, 701, 702, 1174, 1995
AgeI ACCGGT 2 cut(s) 667, 710
AgsI TTSAA 7 cut(s) 430, 655, 827, 1222, 1498, 1735, 1838
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 635, 1867
AlwI GGATC 4 cut(s) 640, 653, 733, 842
AlwNI CAGNNNCTG 2 cut(s) 582, 865
Ama87I CYCGRG 1 cut(s) 1423
ApeKI GCWGC 8 cut(s) 216, 351, 460, 562, 782, 892, 1144, 1767
ApoI RAATTY 4 cut(s) 828, 998, 1337, 1493
ArsI GACNNNNNNTTYG 4 cut(s) 668, 700, 983, 1015
AseI ATTAAT 1 cut(s) 1206
AsiGI ACCGGT 2 cut(s) 667, 710
AspLEI GCGC 4 cut(s) 7, 454, 503, 1772
AsuC2I CCSGG 4 cut(s) 144, 341, 539, 1924
AsuHPI GGTGA 3 cut(s) 214, 1816, 2096
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 1423
AvaII GGWCC 6 cut(s) 87, 184, 670, 1107, 1284, 1873
BaeGI GKGCMC 1 cut(s) 1321
BamHI GGATCC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 102, 315
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 8 cut(s) 203, 363, 472, 549, 769, 904, 1131, 1779
BccI CCATC 8 cut(s) 64, 236, 283, 932, 1098, 1326, 1329, 1410
BceAI ACGGC 4 cut(s) 252, 329, 334, 1788
BcgI CGANNNNNNTGC 4 cut(s) 40, 74, 1424, 1458
BciVI GTATCC 1 cut(s) 2030
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 4 cut(s) 144, 341, 539, 1924
BcoDI GTCTC 2 cut(s) 635, 1867
BfaI CTAG 3 cut(s) 1085, 1101, 2072
BfmI CTRYAG 4 cut(s) 458, 563, 783, 1145
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 1140, 2080
BfuI GTATCC 1 cut(s) 2030
BglI GCCNNNNNGGC 1 cut(s) 206
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 4 cut(s) 144, 341, 539, 1924
Bme18I GGWCC 6 cut(s) 87, 184, 670, 1107, 1284, 1873
BmeT110I CYCGRG 1 cut(s) 1423
BmiI GGNNCC 9 cut(s) 6, 109, 185, 235, 647, 672, 904, 1428, 2000
BmrFI CCNGG 4 cut(s) 144, 341, 539, 1924
BmsI GCATC 4 cut(s) 263, 463, 931, 1858
BoxI GACNNNNGTC 1 cut(s) 1289
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 2013
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 944, 1031
BpuMI CCSGG 4 cut(s) 144, 341, 539, 1924
BsaHI GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
BsaI GGTCTC 1 cut(s) 1867
BsaJI CCNNGG 7 cut(s) 46, 90, 154, 316, 723, 1327, 1989
BsaWI WCCGGW 4 cut(s) 648, 667, 710, 1378
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 285, 680, 701, 702, 1174, 1995
Bse118I RCCGGY 6 cut(s) 212, 354, 448, 667, 710, 918
Bse1I ACTGG 4 cut(s) 89, 576, 806, 1366
Bse3DI GCAATG 4 cut(s) 246, 757, 975, 1081
BseDI CCNNGG 7 cut(s) 46, 90, 154, 316, 723, 1327, 1989
BseGI GGATG 6 cut(s) 294, 850, 1129, 1337, 1590, 1629
BseLI CCNNNNNNNGG 7 cut(s) 256, 285, 680, 701, 702, 1174, 1995
BseMI GCAATG 4 cut(s) 246, 757, 975, 1081
BseMII CTCAG 3 cut(s) 1824, 1878, 2027
BseNI ACTGG 4 cut(s) 89, 576, 806, 1366
BseRI GAGGAG 3 cut(s) 179, 182, 2011
BseSI GKGCMC 1 cut(s) 1321
BseX3I CGGCCG 2 cut(s) 283, 342
BseXI GCAGC 8 cut(s) 203, 363, 472, 549, 769, 904, 1131, 1779
BseYI CCCAGC 2 cut(s) 244, 1663
BsgI GTGCAG 1 cut(s) 563
Bsh1236I CGCG 5 cut(s) 48, 273, 503, 927, 1991
Bsh1285I CGRYCG 2 cut(s) 286, 345
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 2 cut(s) 667, 710
BsiEI CGRYCG 2 cut(s) 286, 345
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 1423
BslFI GGGAC 3 cut(s) 656, 1043, 1240
BslI CCNNNNNNNGG 7 cut(s) 256, 285, 680, 701, 702, 1174, 1995
BsmAI GTCTC 2 cut(s) 635, 1867
BsmFI GGGAC 3 cut(s) 656, 1043, 1240
Bso31I GGTCTC 1 cut(s) 1867
BsoBI CYCGRG 1 cut(s) 1423
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 3 cut(s) 102, 315, 1321
Bsp143I GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
Bsp19I CCATGG 2 cut(s) 90, 723
BspCNI CTCAG 3 cut(s) 1823, 1877, 2026
BspFNI CGCG 5 cut(s) 48, 273, 503, 927, 1991
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 9 cut(s) 6, 109, 185, 235, 647, 672, 904, 1428, 2000
BspMAI CTGCAG 4 cut(s) 462, 567, 787, 1149
BspMI ACCTGC 2 cut(s) 1140, 2080
BspPI GGATC 4 cut(s) 640, 653, 733, 842
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 1867
BsrDI GCAATG 4 cut(s) 246, 757, 975, 1081
BsrFI RCCGGY 6 cut(s) 212, 354, 448, 667, 710, 918
BsrI ACTGG 4 cut(s) 89, 576, 806, 1366
BssAI RCCGGY 6 cut(s) 212, 354, 448, 667, 710, 918
BssECI CCNNGG 7 cut(s) 46, 90, 154, 316, 723, 1327, 1989
BssMI GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
BssNI GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
BssT1I CCWWGG 2 cut(s) 90, 723
Bst4CI ACNGT 2 cut(s) 1057, 1291
Bst6I CTCTTC 1 cut(s) 1517
BstACI GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 214, 437, 450, 1458
BstDEI CTNAG 5 cut(s) 1791, 1810, 1831, 1864, 2013
BstDSI CCRYGG 7 cut(s) 46, 90, 154, 316, 723, 1327, 1989
BstF5I GGATG 6 cut(s) 294, 850, 1129, 1337, 1590, 1629
BstFNI CGCG 5 cut(s) 48, 273, 503, 927, 1991
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 4 cut(s) 7, 454, 503, 1772
BstKTI GATC 8 cut(s) 648, 663, 741, 850, 870, 1479, 1746, 1822
BstMAI GTCTC 2 cut(s) 635, 1867
BstMBI GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
BstMCI CGRYCG 2 cut(s) 286, 345
BstMWI GCNNNNNNNGC 7 cut(s) 206, 445, 460, 1141, 1316, 1426, 1673
BstPAI GACNNNNGTC 1 cut(s) 1289
BstSCI CCNGG 4 cut(s) 142, 339, 537, 1922
BstSFI CTRYAG 4 cut(s) 458, 563, 783, 1145
BstSLI GKGCMC 1 cut(s) 1321
BstUI CGCG 5 cut(s) 48, 273, 503, 927, 1991
BstV1I GCAGC 8 cut(s) 203, 363, 472, 549, 769, 904, 1131, 1779
BstX2I RGATCY 2 cut(s) 645, 738
BstYI RGATCY 2 cut(s) 645, 738
BstZI CGGCCG 2 cut(s) 283, 342
BsuI GTATCC 1 cut(s) 2030
BtgI CCRYGG 7 cut(s) 46, 90, 154, 316, 723, 1327, 1989
BtgZI GCGATG 1 cut(s) 908
BtsCI GGATG 6 cut(s) 294, 850, 1129, 1337, 1590, 1629
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 5 cut(s) 792, 1287, 1373, 1728, 1872
BveI ACCTGC 2 cut(s) 1140, 2080
Cac8I GCNNGC 4 cut(s) 214, 437, 450, 1458
CaiI CAGNNNCTG 2 cut(s) 582, 865
CciI TCATGA 1 cut(s) 597
CfoI GCGC 4 cut(s) 7, 454, 503, 1772
Cfr10I RCCGGY 6 cut(s) 212, 354, 448, 667, 710, 918
Cfr42I CCGCGG 2 cut(s) 49, 1992
CseI GACGC 6 cut(s) 262, 308, 310, 468, 865, 1025
Csp6I GTAC 3 cut(s) 469, 916, 943
CspAI ACCGGT 2 cut(s) 667, 710
CviAII CATG 9 cut(s) 91, 598, 724, 745, 1069, 1300, 1579, 1642, 1717
CviQI GTAC 3 cut(s) 469, 916, 943
DdeI CTNAG 5 cut(s) 1791, 1810, 1831, 1864, 2013
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 647, 662, 740, 849, 869, 1478, 1745, 1821
DpnII GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 4 cut(s) 283, 342, 803, 1801
EagI CGGCCG 2 cut(s) 283, 342
Eam1104I CTCTTC 1 cut(s) 1517
EarI CTCTTC 1 cut(s) 1517
EciI GGCGGA 2 cut(s) 254, 546
Ecl136II GAGCTC 1 cut(s) 100
EclXI CGGCCG 2 cut(s) 283, 342
Eco130I CCWWGG 2 cut(s) 90, 723
Eco24I GRGCYC 2 cut(s) 102, 315
Eco31I GGTCTC 1 cut(s) 1867
Eco47I GGWCC 6 cut(s) 87, 184, 670, 1107, 1284, 1873
Eco52I CGGCCG 2 cut(s) 283, 342
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 1423
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 3 cut(s) 107, 184, 1427
EcoRI GAATTC 1 cut(s) 998
EcoT14I CCWWGG 2 cut(s) 90, 723
EcoT38I GRGCYC 2 cut(s) 102, 315
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 90, 723
FaeI CATG 9 cut(s) 94, 601, 727, 748, 1072, 1303, 1582, 1645, 1720
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 3 cut(s) 656, 1043, 1240
FatI CATG 9 cut(s) 90, 597, 723, 744, 1068, 1299, 1578, 1641, 1716
FauI CCCGC 1 cut(s) 1642
FbaI TGATCA 1 cut(s) 867
FokI GGATG 6 cut(s) 301, 857, 1136, 1344, 1577, 1616
FriOI GRGCYC 2 cut(s) 102, 315
FspBI CTAG 3 cut(s) 1085, 1101, 2072
GlaI GCGC 4 cut(s) 6, 453, 502, 1771
GsaI CCCAGC 2 cut(s) 248, 1667
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 8
HgaI GACGC 6 cut(s) 262, 308, 310, 468, 865, 1025
HhaI GCGC 4 cut(s) 7, 454, 503, 1772
Hin1I GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
Hin1II CATG 9 cut(s) 94, 601, 727, 748, 1072, 1303, 1582, 1645, 1720
Hin6I GCGC 4 cut(s) 5, 452, 501, 1770
HinP1I GCGC 4 cut(s) 5, 452, 501, 1770
HindIII AAGCTT 1 cut(s) 1392
HinfI GANTC 5 cut(s) 137, 601, 823, 839, 1382
HphI GGTGA 3 cut(s) 214, 1816, 2096
Hpy166II GTNNAC 5 cut(s) 177, 916, 1117, 1192, 1554
Hpy8I GTNNAC 5 cut(s) 177, 916, 1117, 1192, 1554
HpyAV CCTTC 8 cut(s) 98, 674, 1552, 1687, 1709, 1882, 1985, 2012
HpyCH4III ACNGT 2 cut(s) 1057, 1291
HpyCH4IV ACGT 3 cut(s) 306, 773, 1356
HpyF10VI GCNNNNNNNGC 7 cut(s) 206, 445, 460, 1141, 1316, 1426, 1673
HpyF3I CTNAG 5 cut(s) 1791, 1810, 1831, 1864, 2013
HpySE526I ACGT 3 cut(s) 306, 773, 1356
Hsp92I GRCGYC 7 cut(s) 5, 300, 306, 321, 479, 773, 1356
Hsp92II CATG 9 cut(s) 94, 601, 727, 748, 1072, 1303, 1582, 1645, 1720
HspAI GCGC 4 cut(s) 5, 452, 501, 1770
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 2 cut(s) 212, 448
KroNI GCCGGC 2 cut(s) 214, 450
Ksp22I TGATCA 1 cut(s) 867
KspI CCGCGG 2 cut(s) 49, 1992
Kzo9I GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
LmnI GCTCC 5 cut(s) 239, 559, 902, 1909, 1998
Lsp1109I GCAGC 8 cut(s) 203, 363, 472, 549, 769, 904, 1131, 1779
LweI GCATC 4 cut(s) 263, 463, 931, 1858
MaeI CTAG 3 cut(s) 1085, 1101, 2072
MaeII ACGT 3 cut(s) 306, 773, 1356
MaeIII GTNAC 4 cut(s) 713, 1625, 1723, 1786
MalI GATC 8 cut(s) 647, 662, 740, 849, 869, 1478, 1745, 1821
MboI GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
MboII GAAGA 9 cut(s) 389, 392, 670, 826, 1204, 1504, 1904, 1939, 1942
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 2 cut(s) 645, 738
MhlI GDGCHC 3 cut(s) 102, 315, 1321
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 138, 875, 1236
MroNI GCCGGC 2 cut(s) 212, 448
MseI TTAA 4 cut(s) 495, 1155, 1206, 1502
MslI CAYNNNNRTG 4 cut(s) 68, 1583, 1721, 1777
MspA1I CMGCKG 6 cut(s) 18, 48, 463, 562, 1232, 1991
MspR9I CCNGG 4 cut(s) 144, 341, 539, 1924
MunI CAATTG 1 cut(s) 777
MvnI CGCG 5 cut(s) 48, 273, 503, 927, 1991
MwoI GCNNNNNNNGC 7 cut(s) 206, 445, 460, 1141, 1316, 1426, 1673
NaeI GCCGGC 2 cut(s) 214, 450
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 144, 341, 539, 1924
NcoI CCATGG 2 cut(s) 90, 723
NdeII GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
NgoMIV GCCGGC 2 cut(s) 212, 448
NlaIII CATG 9 cut(s) 94, 601, 727, 748, 1072, 1303, 1582, 1645, 1720
NlaIV GGNNCC 9 cut(s) 6, 109, 185, 235, 647, 672, 904, 1428, 2000
NmeAIII GCCGAG 1 cut(s) 1627
NmuCI GTSAC 2 cut(s) 1723, 1786
NspV TTCGAA 1 cut(s) 521
PagI TCATGA 1 cut(s) 597
PaqCI CACCTGC 1 cut(s) 2080
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 1011
PdiI GCCGGC 2 cut(s) 214, 450
PfeI GAWTC 4 cut(s) 601, 823, 839, 1382
PfoI TCCNGGA 1 cut(s) 1922
PinAI ACCGGT 2 cut(s) 667, 710
PleI GAGTC 1 cut(s) 145
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 1289
PshBI ATTAAT 1 cut(s) 1206
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 184
PspFI CCCAGC 2 cut(s) 244, 1663
PspN4I GGNNCC 9 cut(s) 6, 109, 185, 235, 647, 672, 904, 1428, 2000
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 4 cut(s) 462, 567, 787, 1149
PstNI CAGNNNCTG 2 cut(s) 582, 865
PsuI RGATCY 2 cut(s) 645, 738
PvuII CAGCTG 3 cut(s) 18, 463, 1232
RsaI GTAC 3 cut(s) 470, 917, 944
RsaNI GTAC 3 cut(s) 469, 916, 943
RseI CAYNNNNRTG 4 cut(s) 68, 1583, 1721, 1777
SacI GAGCTC 1 cut(s) 102
SacII CCGCGG 2 cut(s) 49, 1992
SaqAI TTAA 4 cut(s) 495, 1155, 1206, 1502
Sau3AI GATC 8 cut(s) 645, 660, 738, 847, 867, 1476, 1743, 1819
ScaI AGTACT 1 cut(s) 470
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 4 cut(s) 144, 341, 539, 1924
SduI GDGCHC 3 cut(s) 102, 315, 1321
SfaNI GCATC 4 cut(s) 263, 463, 931, 1858
SfcI CTRYAG 4 cut(s) 458, 563, 783, 1145
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 2 cut(s) 49, 1992
SfuI TTCGAA 1 cut(s) 521
SgrBI CCGCGG 2 cut(s) 49, 1992
SinI GGWCC 6 cut(s) 87, 184, 670, 1107, 1284, 1873
SmiMI CAYNNNNRTG 4 cut(s) 68, 1583, 1721, 1777
SmlI CTYRAG 2 cut(s) 959, 1046
SmoI CTYRAG 2 cut(s) 959, 1046
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 1085, 1101, 2072
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 4 cut(s) 142, 339, 537, 1922
StyI CCWWGG 2 cut(s) 90, 723
TaaI ACNGT 2 cut(s) 1057, 1291
TaiI ACGT 3 cut(s) 309, 776, 1359
TaqII GACCGA 1 cut(s) 2057
TatI WGTACW 1 cut(s) 468
TauI GCSGC 6 cut(s) 51, 212, 422, 562, 1991, 1994
TfiI GAWTC 4 cut(s) 601, 823, 839, 1382
Tru1I TTAA 4 cut(s) 495, 1155, 1206, 1502
Tru9I TTAA 4 cut(s) 495, 1155, 1206, 1502
TscAI CASTG 5 cut(s) 792, 1294, 1373, 1728, 1872
TseFI GTSAC 2 cut(s) 1723, 1786
TseI GCWGC 8 cut(s) 216, 351, 460, 562, 782, 892, 1144, 1767
Tsp45I GTSAC 2 cut(s) 1723, 1786
TspGWI ACGGA 2 cut(s) 143, 1344
TspRI CASTG 5 cut(s) 792, 1294, 1373, 1728, 1872
VpaK11BI GGWCC 6 cut(s) 87, 184, 670, 1107, 1284, 1873
VspI ATTAAT 1 cut(s) 1206
XapI RAATTY 4 cut(s) 828, 998, 1337, 1493
XbaI TCTAGA 1 cut(s) 2071
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 3 cut(s) 1085, 1101, 2072
ZraI GACGTC 3 cut(s) 307, 774, 1357
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.