MD15G1064400.v1.1

xyloglucan glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014524
Physical Location & Seq
Forward (+)
4476878 .. 4480025
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1064400.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGCGCCCTCCTTCAGCTGGTGGAATAATAATAAAGACACCCACCGCGGCACTCCCGTCGTCGTCAAGATGGAGAACCCCAACTGGTCCATGGTCGAGCTCGAAGGCCCCACCGACAAGGACTTCCTCATCTCCGAGTCGCCTGGTCGGGTCCGTGGCAAAAACGCCAAGCAGTTCACTTGGGTCCTCCTCCTCAGAGCCCACAGAGCCGCCGGCTGCCTCACCTCCATTGGCTCCGCAATGCTGGGCCTCGCATCCGCAATCCGCCGCCGCATCGCCTCCGGCAGGATGGACACCGACGCCGACGTCGGGCCCGCCAGCGTCAGGGAAGTCGAAAACCCGGCCGTCCGGAGCCGGTTCTATTTCTGCATCAAGATGATCCTGTGGCTGTCGGTGCTCCTTCTGGGGTTTGAGGTGGCGGCGTATTTCAAAGGCTGGCACTTTGGGGCGCCGCATCTGCAGCTGGAGTATTTGTGGGCGTCGCCATTGGGGGTTAAGGGCGCATTCGATTGGGTTTATTCGAAATGGGTATTTTTCCGGGTTGAATATTTAGCTCCGCCGCTGCAGTTTCTCGCCAGTTCCTGCATTGTTCTGTTCATGATTCAGAGCTTGGATAGGCTAATCCTCTGTCTGGGTTGCTTCTGGATCCGGTTCAAGAATATCAAACCGGTCCCAAAAGAAGACGCTTTCGACCCCGAAACAGGGGAAACCGGTTACTTCCCCATGGTTCTTGTTCAGATCCCCATGTGCAATGAAAAGGAGGTTTATCAGACGTCAATTGCTGCAGTGTGCAATTTGGACTGGCCGAAATCTAAGCTGTTGATTCAAATTCTCGATGATTCGGATGACCCAACCACGCAGTTTCTGATCAAAGAGGAAGTTCATAAGTGGCAGCAGGAGGGAGCCAACATTCTGTACCGGCATCGAGTGATTCGAGACGGATACAAGGCTGGTAATCTCAAGTCTGCAATGCATTGTAGCTATGTCAAAGACTATGAATTCGTGGCGATTTTTGACGCAGATTTTCAGCCCACCCCAGACTTCCTCAAGAGAACAGTGCCTCACTTCAGGGGCAATGATGAACTAGGGCTGGTTCAGGCTAGGTGGTCCTTTGTGAACAAGGATGAGAACCTACTCACAAGGCTGCAGAACATTAATTTGTCCTTCCACTTTGAGGTTGAGCAACAAGTGAACAGCGTGTTCATTAATTTCTTCGGTTTCAATGGGACAGCTGGTGTGTGGAGGATAAAGGCGTTGGAGGAAGCTGGTGGGTGGTTGGAGAGGACCACTGTCGAGGACATGGATATTGCTGTTCGTGCCCACCTCCACGGATGGAAATTCATTTTCCTCAATGACGTCGAGTGCCAGTGTGAATTACCCGAGTCTTATGAAGCTTATCGGAAGCAACAACACCGATGGCACTCGGGGCCTATGCAATTATTTCGCCTCTGTTTGCCCGCTATTATAAAATCTAAGATCAGCATTGGCAAGAAATTCAATTTGATCTTTCTCTTCTTTCTGCTCCGGAAACTTATATTACCCTTCTACTCGTTTACGCTTTTTTGCATAATTCTCCCCATGACAATGTTCATCCCAGAGGCCGAGCTACCAGCGTGGGTTGTATGCTACATCCCCGCCACCATGTCGTTTCTCAACATCCTCCCAGCCCCAAAAGCCTTCCCTTTCATTGTTCCTTACCTTCTCTTTGAGAACACCATGTCGGTAACAAAATTCAACGCTATGATCTCCGGCCTGTTTCAGCTTGGCAGCGCATACGAGTGGGTTGTCACTAAGAAATCCGGCCGTTCCTCAGAGGGCGATCTCACCTCCTTAGTTGAGAAGCCAAAGCATCAAAGGGGGGTCTCAGTGCCGGACCTTGTTGAAATGAAGGAAGTAATTAGACAGCAGGAGCAAAGGGCTTCCAAGAAAAAGAAGCACAACAGAATATACACAAAGGAGTTGGCATTGGCATTCCTCCTTCTAACTGCTGCGGCGAGGAGCCTTCTGTCTGCTCAGGGTATCCACTTCTACTTCTTGTTGTTTCAGGGAGTATCGTTCCTGCTGGTCGGTTTAGACCTAATCGGCGAACAGGTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_tranf_2_3 Glyco_trans_2_3

Protein Analysis

700

Amino Acids

80.14

Weight (kDa)

8.6

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 241 - 474 2.3e-13 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 246 - 389 2.1e-10 Glycosyl transferase family 2
Glyco_transf_21 PF13506 330 - 474 1.1e-07 Glycosyl transferase family 21
Glyco_trans_2_3 PF13632 335 - 543 6.1e-21 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G07960 AT4G07960
fragaria_vesca FvH4_2g32570
malus_domestica MD08G1076900.v1.1 MD15G1064400.v1.1
prunus_persica Prupe.1G418400_v2.0.a1
pyrus_communis pycom08g06370 pycom15g06110
rosa_chinensis RchiOBHm_Chr6g0310111
rosa_laevigata RLG00000010510
rosa_multiflora Rmu_sc0000294.1_g000027
rosa_roxburghii Rroxscaffold_7G00158860
rosa_rugosa Rorug06G0377600
rosa_samantha Rh6BG500800 Rh6CG505900 Rh6DG491800
rosa_wichuraiana Rw6G042760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1466
AasI GACNNNNNNGTC 1 cut(s) 305
AatII GACGTC 3 cut(s) 309, 776, 1359
AccB1I GGYRCC 2 cut(s) 4, 448
AccII CGCG 1 cut(s) 48
AccIII TCCGGA 2 cut(s) 348, 1524
AclWI GGATC 4 cut(s) 373, 640, 653, 733
AcoI YGGCCR 3 cut(s) 342, 803, 1801
AcsI RAATTY 5 cut(s) 828, 998, 1337, 1493, 1730
AcuI CTGAAG 1 cut(s) 1051
AcyI GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
AfaI GTAC 1 cut(s) 917
AfiI CCNNNNNNNGG 8 cut(s) 18, 285, 309, 324, 631, 701, 702, 1174
AgeI ACCGGT 2 cut(s) 667, 710
AgsI TTSAA 8 cut(s) 430, 545, 655, 827, 1222, 1498, 1735, 1883
AjnI CCWGG 1 cut(s) 142
Alw21I GWGCWC 2 cut(s) 102, 399
Alw26I GTCTC 2 cut(s) 930, 1867
AlwI GGATC 4 cut(s) 373, 640, 653, 733
AlwNI CAGNNNCTG 2 cut(s) 582, 865
Ama87I CYCGRG 2 cut(s) 1379, 1423
Aor13HI TCCGGA 2 cut(s) 348, 1524
AoxI GGCC 9 cut(s) 106, 247, 311, 342, 803, 1427, 1599, 1750, 1801
ApaI GGGCCC 1 cut(s) 315
ApeKI GCWGC 8 cut(s) 216, 460, 562, 782, 892, 1144, 1767, 1988
ApoI RAATTY 5 cut(s) 828, 998, 1337, 1493, 1730
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
AseI ATTAAT 2 cut(s) 1155, 1206
AsiGI ACCGGT 2 cut(s) 667, 710
AspLEI GCGC 4 cut(s) 7, 451, 503, 1772
AsuC2I CCSGG 2 cut(s) 341, 539
AsuHPI GGTGA 2 cut(s) 214, 1816
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 2 cut(s) 1379, 1423
AvaII GGWCC 7 cut(s) 87, 151, 184, 670, 1107, 1284, 1873
BaeGI GKGCMC 2 cut(s) 315, 1321
BamHI GGATCC 1 cut(s) 645
BanI GGYRCC 2 cut(s) 4, 448
BanII GRGCYC 3 cut(s) 102, 202, 315
BbsI GAAGAC 1 cut(s) 687
Bbv12I GWGCWC 2 cut(s) 102, 399
BbvI GCAGC 8 cut(s) 203, 472, 549, 769, 904, 1131, 1779, 1975
BccI CCATC 4 cut(s) 64, 283, 1326, 1410
BceAI ACGGC 2 cut(s) 329, 1788
BcgI CGANNNNNNTGC 4 cut(s) 40, 74, 1424, 1458
BciT130I CCWGG 1 cut(s) 144
BciVI GTATCC 2 cut(s) 935, 2030
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 2 cut(s) 341, 539
BcoDI GTCTC 2 cut(s) 930, 1867
BfaI CTAG 2 cut(s) 1085, 1101
BfmI CTRYAG 4 cut(s) 458, 563, 783, 1145
BfoI RGCGCY 2 cut(s) 8, 452
BfuI GTATCC 2 cut(s) 935, 2030
Bme1390I CCNGG 3 cut(s) 144, 341, 539
Bme18I GGWCC 7 cut(s) 87, 151, 184, 670, 1107, 1284, 1873
BmeT110I CYCGRG 2 cut(s) 1379, 1423
BmrFI CCNGG 3 cut(s) 144, 341, 539
BmsI GCATC 6 cut(s) 263, 282, 378, 463, 931, 1858
BoxI GACNNNNGTC 1 cut(s) 1289
BpiI GAAGAC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 2013
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 2 cut(s) 944, 1031
BpuMI CCSGG 2 cut(s) 341, 539
BsaHI GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
BsaI GGTCTC 1 cut(s) 1867
BsaJI CCNNGG 5 cut(s) 46, 90, 154, 723, 1327
BsaWI WCCGGW 5 cut(s) 348, 648, 667, 710, 1524
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 8 cut(s) 18, 285, 309, 324, 631, 701, 702, 1174
Bse118I RCCGGY 5 cut(s) 212, 354, 667, 710, 918
Bse1I ACTGG 4 cut(s) 89, 576, 806, 1366
Bse3DI GCAATG 4 cut(s) 246, 757, 975, 1081
BseAI TCCGGA 2 cut(s) 348, 1524
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 5 cut(s) 46, 90, 154, 723, 1327
BseGI GGATG 8 cut(s) 254, 294, 850, 1129, 1337, 1590, 1629, 1656
BseLI CCNNNNNNNGG 8 cut(s) 18, 285, 309, 324, 631, 701, 702, 1174
BseMI GCAATG 4 cut(s) 246, 757, 975, 1081
BseMII CTCAG 4 cut(s) 208, 1824, 1878, 2027
BseNI ACTGG 4 cut(s) 89, 576, 806, 1366
BseRI GAGGAG 3 cut(s) 179, 182, 2011
BseSI GKGCMC 2 cut(s) 315, 1321
BseX3I CGGCCG 2 cut(s) 342, 1801
BseXI GCAGC 8 cut(s) 203, 472, 549, 769, 904, 1131, 1779, 1975
BseYI CCCAGC 2 cut(s) 244, 1663
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 2 cut(s) 345, 1804
BshFI GGCC 9 cut(s) 108, 249, 313, 344, 805, 1429, 1601, 1752, 1803
BshNI GGYRCC 2 cut(s) 4, 448
BshTI ACCGGT 2 cut(s) 667, 710
BsiEI CGRYCG 2 cut(s) 345, 1804
BsiHKAI GWGCWC 2 cut(s) 102, 399
BsiHKCI CYCGRG 2 cut(s) 1379, 1423
BslFI GGGAC 2 cut(s) 656, 1240
BslI CCNNNNNNNGG 8 cut(s) 18, 285, 309, 324, 631, 701, 702, 1174
BsmAI GTCTC 2 cut(s) 930, 1867
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 656, 1240
BsmI GAATGC 2 cut(s) 503, 1970
BsnI GGCC 9 cut(s) 108, 249, 313, 344, 805, 1429, 1601, 1752, 1803
Bso31I GGTCTC 1 cut(s) 1867
BsoBI CYCGRG 2 cut(s) 1379, 1423
Bsp119I TTCGAA 1 cut(s) 521
Bsp120I GGGCCC 1 cut(s) 311
Bsp1286I GDGCHC 5 cut(s) 102, 202, 315, 399, 1321
Bsp13I TCCGGA 2 cut(s) 348, 1524
Bsp143I GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
Bsp19I CCATGG 2 cut(s) 90, 723
BspANI GGCC 9 cut(s) 108, 249, 313, 344, 805, 1429, 1601, 1752, 1803
BspCNI CTCAG 4 cut(s) 207, 1823, 1877, 2026
BspEI TCCGGA 2 cut(s) 348, 1524
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 597
BspMAI CTGCAG 4 cut(s) 462, 567, 787, 1149
BspPI GGATC 4 cut(s) 373, 640, 653, 733
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 2 cut(s) 4, 448
BspTNI GGTCTC 1 cut(s) 1867
BsrDI GCAATG 4 cut(s) 246, 757, 975, 1081
BsrFI RCCGGY 5 cut(s) 212, 354, 667, 710, 918
BsrI ACTGG 4 cut(s) 89, 576, 806, 1366
BssAI RCCGGY 5 cut(s) 212, 354, 667, 710, 918
BssECI CCNNGG 5 cut(s) 46, 90, 154, 723, 1327
BssMI GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
BssNI GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
BssT1I CCWWGG 2 cut(s) 90, 723
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 1057, 1291
Bst6I CTCTTC 1 cut(s) 1517
BstACI GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 5 cut(s) 214, 315, 319, 437, 1458
BstDEI CTNAG 8 cut(s) 194, 813, 1473, 1791, 1810, 1831, 1864, 2013
BstDSI CCRYGG 5 cut(s) 46, 90, 154, 723, 1327
BstENI CCTNNNNNAGG 1 cut(s) 283
BstF5I GGATG 8 cut(s) 254, 294, 850, 1129, 1337, 1590, 1629, 1656
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 2 cut(s) 8, 452
BstHHI GCGC 4 cut(s) 7, 451, 503, 1772
BstKTI GATC 8 cut(s) 381, 648, 741, 870, 1479, 1506, 1746, 1822
BstMAI GTCTC 2 cut(s) 930, 1867
BstMBI GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
BstMCI CGRYCG 2 cut(s) 345, 1804
BstMWI GCNNNNNNNGC 7 cut(s) 206, 394, 457, 460, 1316, 1426, 1673
BstNI CCWGG 1 cut(s) 144
BstPAI GACNNNNGTC 1 cut(s) 1289
BstSCI CCNGG 3 cut(s) 142, 339, 537
BstSFI CTRYAG 4 cut(s) 458, 563, 783, 1145
BstSLI GKGCMC 2 cut(s) 315, 1321
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 8 cut(s) 203, 472, 549, 769, 904, 1131, 1779, 1975
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 2 cut(s) 645, 738
BstYI RGATCY 2 cut(s) 645, 738
BstZI CGGCCG 2 cut(s) 342, 1801
BsuI GTATCC 2 cut(s) 935, 2030
BsuRI GGCC 9 cut(s) 108, 249, 313, 344, 805, 1429, 1601, 1752, 1803
BtgI CCRYGG 5 cut(s) 46, 90, 154, 723, 1327
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 8 cut(s) 254, 294, 850, 1129, 1337, 1590, 1629, 1656
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 5 cut(s) 792, 1062, 1287, 1373, 1872
Cac8I GCNNGC 5 cut(s) 214, 315, 319, 437, 1458
CaiI CAGNNNCTG 2 cut(s) 582, 865
CciI TCATGA 1 cut(s) 597
CfoI GCGC 4 cut(s) 7, 451, 503, 1772
Cfr10I RCCGGY 5 cut(s) 212, 354, 667, 710, 918
Cfr42I CCGCGG 1 cut(s) 49
CseI GACGC 5 cut(s) 308, 310, 468, 692, 1025
Csp6I GTAC 1 cut(s) 916
CspAI ACCGGT 2 cut(s) 667, 710
CviAII CATG 8 cut(s) 91, 598, 724, 745, 1300, 1579, 1642, 1717
CviQI GTAC 1 cut(s) 916
DdeI CTNAG 8 cut(s) 194, 813, 1473, 1791, 1810, 1831, 1864, 2013
DinI GGCGCC 2 cut(s) 6, 450
DpnI GATC 8 cut(s) 380, 647, 740, 869, 1478, 1505, 1745, 1821
DpnII GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 3 cut(s) 342, 803, 1801
EagI CGGCCG 2 cut(s) 342, 1801
Eam1104I CTCTTC 1 cut(s) 1517
EarI CTCTTC 1 cut(s) 1517
EciI GGCGGA 2 cut(s) 254, 546
Ecl136II GAGCTC 1 cut(s) 100
EclXI CGGCCG 2 cut(s) 342, 1801
Eco130I CCWWGG 2 cut(s) 90, 723
Eco24I GRGCYC 3 cut(s) 102, 202, 315
Eco31I GGTCTC 1 cut(s) 1867
Eco47I GGWCC 7 cut(s) 87, 151, 184, 670, 1107, 1284, 1873
Eco52I CGGCCG 2 cut(s) 342, 1801
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 1051
Eco88I CYCGRG 2 cut(s) 1379, 1423
EcoICRI GAGCTC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 283
EcoO109I RGGNCCY 3 cut(s) 107, 184, 1427
EcoRI GAATTC 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 90, 723
EcoT22I ATGCAT 1 cut(s) 975
EcoT38I GRGCYC 3 cut(s) 102, 202, 315
EgeI GGCGCC 2 cut(s) 6, 450
EheI GGCGCC 2 cut(s) 6, 450
ErhI CCWWGG 2 cut(s) 90, 723
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 8 cut(s) 94, 601, 727, 748, 1303, 1582, 1645, 1720
FaqI GGGAC 2 cut(s) 656, 1240
FatI CATG 8 cut(s) 90, 597, 723, 744, 1299, 1578, 1641, 1716
FauI CCCGC 3 cut(s) 322, 1465, 1642
FbaI TGATCA 1 cut(s) 867
FokI GGATG 8 cut(s) 241, 301, 857, 1136, 1344, 1577, 1616, 1643
FriOI GRGCYC 3 cut(s) 102, 202, 315
FspBI CTAG 2 cut(s) 1085, 1101
GlaI GCGC 4 cut(s) 6, 450, 502, 1771
GsaI CCCAGC 2 cut(s) 248, 1667
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 2 cut(s) 8, 452
HaeIII GGCC 9 cut(s) 108, 249, 313, 344, 805, 1429, 1601, 1752, 1803
HgaI GACGC 5 cut(s) 308, 310, 468, 692, 1025
HhaI GCGC 4 cut(s) 7, 451, 503, 1772
Hin1I GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
Hin1II CATG 8 cut(s) 94, 601, 727, 748, 1303, 1582, 1645, 1720
Hin6I GCGC 4 cut(s) 5, 449, 501, 1770
HinP1I GCGC 4 cut(s) 5, 449, 501, 1770
HindIII AAGCTT 1 cut(s) 1392
HinfI GANTC 6 cut(s) 137, 601, 823, 839, 931, 1382
HphI GGTGA 2 cut(s) 214, 1816
Hpy166II GTNNAC 4 cut(s) 177, 1117, 1192, 1554
Hpy188I TCNGA 9 cut(s) 136, 197, 606, 738, 771, 844, 867, 1401, 1813
Hpy8I GTNNAC 4 cut(s) 177, 1117, 1192, 1554
Hpy99I CGWCG 7 cut(s) 62, 65, 302, 308, 311, 484, 1361
HpyCH4III ACNGT 2 cut(s) 1057, 1291
HpyCH4IV ACGT 3 cut(s) 306, 773, 1356
HpyF10VI GCNNNNNNNGC 7 cut(s) 206, 394, 457, 460, 1316, 1426, 1673
HpyF3I CTNAG 8 cut(s) 194, 813, 1473, 1791, 1810, 1831, 1864, 2013
HpySE526I ACGT 3 cut(s) 306, 773, 1356
Hsp92I GRCGYC 7 cut(s) 5, 300, 306, 449, 479, 773, 1356
Hsp92II CATG 8 cut(s) 94, 601, 727, 748, 1303, 1582, 1645, 1720
HspAI GCGC 4 cut(s) 5, 449, 501, 1770
KasI GGCGCC 2 cut(s) 4, 448
Kpn2I TCCGGA 2 cut(s) 348, 1524
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 867
KspI CCGCGG 1 cut(s) 49
Kzo9I GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
LmnI GCTCC 8 cut(s) 239, 351, 402, 559, 902, 1527, 1909, 1998
Lsp1109I GCAGC 8 cut(s) 203, 472, 549, 769, 904, 1131, 1779, 1975
LweI GCATC 6 cut(s) 263, 282, 378, 463, 931, 1858
MaeI CTAG 2 cut(s) 1085, 1101
MaeII ACGT 3 cut(s) 306, 773, 1356
MaeIII GTNAC 3 cut(s) 713, 1723, 1786
MalI GATC 8 cut(s) 380, 647, 740, 869, 1478, 1505, 1745, 1821
MboI GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
MboII GAAGA 3 cut(s) 692, 1204, 1504
MfeI CAATTG 1 cut(s) 777
MflI RGATCY 2 cut(s) 645, 738
MhlI GDGCHC 5 cut(s) 102, 202, 315, 399, 1321
Mly113I GGCGCC 2 cut(s) 5, 449
MlyI GAGTC 2 cut(s) 146, 1391
MmeI TCCRAC 2 cut(s) 1236, 1257
Mph1103I ATGCAT 1 cut(s) 975
MroI TCCGGA 2 cut(s) 348, 1524
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 3 cut(s) 495, 1155, 1206
MslI CAYNNNNRTG 3 cut(s) 374, 1583, 1777
MspA1I CMGCKG 5 cut(s) 18, 48, 463, 562, 1232
MspR9I CCNGG 3 cut(s) 144, 341, 539
MunI CAATTG 1 cut(s) 777
Mva1269I GAATGC 2 cut(s) 503, 1970
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 7 cut(s) 206, 394, 457, 460, 1316, 1426, 1673
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 2 cut(s) 5, 449
NciI CCSGG 2 cut(s) 341, 539
NcoI CCATGG 2 cut(s) 90, 723
NdeII GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 8 cut(s) 94, 601, 727, 748, 1303, 1582, 1645, 1720
NmeAIII GCCGAG 1 cut(s) 1627
NmuCI GTSAC 1 cut(s) 1786
NsiI ATGCAT 1 cut(s) 975
NspV TTCGAA 1 cut(s) 521
PagI TCATGA 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 931
PctI GAATGC 2 cut(s) 503, 1970
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 4 cut(s) 601, 823, 839, 931
PinAI ACCGGT 2 cut(s) 667, 710
PleI GAGTC 2 cut(s) 145, 1390
PluTI GGCGCC 2 cut(s) 8, 452
PpsI GAGTC 2 cut(s) 145, 1390
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 1289
PshBI ATTAAT 2 cut(s) 1155, 1206
PsiI TTATAA 1 cut(s) 1466
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 142
PspFI CCCAGC 2 cut(s) 244, 1663
PspGI CCWGG 1 cut(s) 142
PspOMI GGGCCC 1 cut(s) 311
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 4 cut(s) 462, 567, 787, 1149
PstNI CAGNNNCTG 2 cut(s) 582, 865
PsuI RGATCY 2 cut(s) 645, 738
PvuII CAGCTG 3 cut(s) 18, 463, 1232
RsaI GTAC 1 cut(s) 917
RsaNI GTAC 1 cut(s) 916
RseI CAYNNNNRTG 3 cut(s) 374, 1583, 1777
SacI GAGCTC 1 cut(s) 102
SacII CCGCGG 1 cut(s) 49
SaqAI TTAA 3 cut(s) 495, 1155, 1206
Sau3AI GATC 8 cut(s) 378, 645, 738, 867, 1476, 1503, 1743, 1819
SchI GAGTC 2 cut(s) 146, 1391
ScrFI CCNGG 3 cut(s) 144, 341, 539
SduI GDGCHC 5 cut(s) 102, 202, 315, 399, 1321
SfaNI GCATC 6 cut(s) 263, 282, 378, 463, 931, 1858
SfcI CTRYAG 4 cut(s) 458, 563, 783, 1145
SfoI GGCGCC 2 cut(s) 6, 450
Sfr303I CCGCGG 1 cut(s) 49
SfuI TTCGAA 1 cut(s) 521
SgrBI CCGCGG 1 cut(s) 49
SinI GGWCC 7 cut(s) 87, 151, 184, 670, 1107, 1284, 1873
SmiMI CAYNNNNRTG 3 cut(s) 374, 1583, 1777
SmlI CTYRAG 2 cut(s) 959, 1046
SmoI CTYRAG 2 cut(s) 959, 1046
SspDI GGCGCC 2 cut(s) 4, 448
SspI AATATT 1 cut(s) 548
SspMI CTAG 2 cut(s) 1085, 1101
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 142, 339, 537
StyI CCWWGG 2 cut(s) 90, 723
TaaI ACNGT 2 cut(s) 1057, 1291
TaiI ACGT 3 cut(s) 309, 776, 1359
TauI GCSGC 8 cut(s) 51, 212, 270, 273, 422, 454, 562, 1994
TfiI GAWTC 4 cut(s) 601, 823, 839, 931
Tru1I TTAA 3 cut(s) 495, 1155, 1206
Tru9I TTAA 3 cut(s) 495, 1155, 1206
TscAI CASTG 5 cut(s) 792, 1062, 1294, 1373, 1872
TseFI GTSAC 1 cut(s) 1786
TseI GCWGC 8 cut(s) 216, 460, 562, 782, 892, 1144, 1767, 1988
Tsp45I GTSAC 1 cut(s) 1786
TspGWI ACGGA 3 cut(s) 143, 954, 1344
TspRI CASTG 5 cut(s) 792, 1062, 1294, 1373, 1872
VpaK11BI GGWCC 7 cut(s) 87, 151, 184, 670, 1107, 1284, 1873
VspI ATTAAT 2 cut(s) 1155, 1206
XagI CCTNNNNNAGG 1 cut(s) 283
XapI RAATTY 5 cut(s) 828, 998, 1337, 1493, 1730
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 2 cut(s) 1085, 1101
ZraI GACGTC 3 cut(s) 307, 774, 1357
Zsp2I ATGCAT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.