Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009337

Physical Location & Seq

Genomic Coordinates Forward (+)

6688051 .. 6693304

Gene Structure 5254 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1080300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2478 bp

ATGTCTGAAGCTTACAGGGCGAGTTCCGTCGATTGGAAGCCCTCTCCCTTGGTTGCCCTAGCCACCAGCGTCGACGACTCCCAAGTCGCCGCTGCTCGCCAGGACGGCTCCCTCGAGATTTGGCTCGTCTCCCCCGGCTCCGTCGGCTGGCACTGTCAGCTGACGATTCATGGTGATCCTGAATCGAGAGTCTCGTCGCTTATTTGGTGTCGTGCTGGCTCGAAAGGGTTGCCTTGTGGACGGTTGTTCTCGTCGAGCATCAACGGTTCGGTTTCGCAGTGGGATCTTTTCCATTTGAAGCAGAAGACCGTGTTGGATTCTATTGGGGTCTCAATCTGGCAAATGGCTGTTGCACCGTGTACTAATGAAACAGAACCTAGGGACCACCCTATTGGCAATGGGTTTCTTAAAGCTAATAGTACCGAGGGGGATGATCCAGAAACCAGTGATAGCGAAGATGATTCTGATTCAGCTGAGATTAATGAGCAATCAGTAGTTGAGAACCAGCGTGTGGCATTAGCTTGTGATGATGGCTGTGTGCGGATATACACCATTACCAACACAGATGAGTTTGTATACACTAAATCATTGCCTAGGGTCAGTGGACGTGTCTTGAGTGTGACATGGAGTCCTGATGCTAAATTTATATATTTGGGGAGTAGTCATGGGATGATAAGATGTTGGGATGCTAAATTGGGCCATGAAATTTATAGGATTACGGTTGGGCTTGGAGGTTTGGGTAGTGGACCTGAACTTTGCGTTTGGTCGTTACTTTCTCTGAGGTGTGGGAACCTTGTAAGTGCAGACAGTACTGGCAGTGTTCAGTTTTGGGACAGTCAGCATGGAACTCTATTGCAGGCACATTCGTACCACAAAGGTGATGTAAATGCTCTGGCAGCAGCTCCCAGCCATAATAGGGTGTTTTCTGCTGGTTCCGATGGTCAGGTTATTCTTTATAAGCTCTCTAGTGCGACAGTGGGATCTAGTGATGACAAGTCTTCTTCTGAGGTGACGAAGAAATGGATCTATGTTGGCCTTGTAAAGGCTCATACACATGATGTTAGGGCTTTGACAGTGGCTGTACCGATCAGCAGAGAAGATCCCTTGCCAGATGAAGGTATAAAAATACACCGTAAAGAACGTCGTAAGATAACGTCTAATAATATTAAGGAGTTCAGTTATCGTAAATGGGCACATTTGGGTGTTCCCATGCTTATCTCTGCTGGTGATGACACAAAGCTAATCGCTTATCCTGTTAAGGAGTTCACACAGTTTAAACCCCATGATATCTGCCCTGCACCTCAAAGACTACCAATTCAACTGGTGCTTAATAGAACATCCAAAGAATCATTGCTTCTTGTACAAGCTTCTAGTTGGGTGGATATCCTGCTTGTACGTACACAAAGTGGCACCTTTTCTAAGATGGCTCCTGTCTCCAAATGGGGCCAAGCCTCAACAGATCTTTTGGTTCGAGTCAAGAGTAAGGCATCTCGGAAGATCATTTGCAGCGCAATTTCTAGTACGGGGGTGCTTTTTGCTTATTCTGACCATGTGAAACCTAGCCTATATGAATTGAAGAAGAATAAAGTTGGCAAAAGTGCACTGACTGTTAATAAAAGACAACTTCCACAGAAACTACCATTTGGCCATTCCATGGTTTTTAGTTCTGATTCTTCTCACTTAATGATAGCAGGGCATGACAGAAGAATATATGTTGTGGATGTGAGCAGAGAAGAACTCGTGCACAGGTTCACCCCTTGTCGCGATTTGCAGGATCAAGAATTACCACCTAGTGAGCCTCCCATAACAAAACTGTTCACCAGTTCTGATGGGCAGTGGCTGGCTGCCATCAACTGCTTTGGAGATATTTATGTATTTAATCTCGAGATACAAAGGCAGCACTGGTTCATTTCAAGACTAAATGGTGCCTCTGTGACAGCTGGTGGTTTTTCCCCACGAAACAACAATGTGCTTGTAATCACAACCTCTTCAAATCATGTATATGCGTTGGATGTTGAGGAGAGAGAACTGGGAAACTGGTCGAGGCAGCATACATATTGTCTGCCAAAGAAATTCCAGGAATTTCCAGGCGAAGTTATCGGGCTCTCTTTCCCACCCTCCACAAGTTCATCTTCTGTTATCGTTTACAGTGCCAGGGCAATGTGCTGGATTGATTTCGGGAAGCCCATTGATCCAGATGATGAAAGTAGTTTCAAGCCAAACGGTCAGAATCCGACATCGTTGAATTTGCAAAGTAGTAATTCCATTAAGAAGATTCTAAAACGTAAATTGACAGACAGTGAAACCGAGAGTAAACTTACGGCTATGAAGAATTTTGATTTTATTTGGTTCCCGAGTCCAGCTTTATTTTTAGGTCATCTTTCGAATAGTTCTCTGTTGATGATAGACAAACCGTGGATGGAAGTGGTTAAATCTTTGGATTCTGCACCCGTACACAAACATGTTTACGGGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010015 GO:0010051 GO:0010073 GO:0010101 GO:0010102 GO:0010154 GO:0010305 GO:0010311 GO:0010449 GO:0010817 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0032879 GO:0035266 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0051049 GO:0061458 GO:0065007 GO:0065008 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393 GO:2000012

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

826

Amino Acids

91.11

Weight (kDa)

7.6

Isoelectric Point (pI)

39.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	166 - 321	6.9e-10	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	167 - 326	1.7e-11	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	168 - 328	2.8e-10	CDC20/Fizzy WD40 domain
WDR55	PF24796	168 - 338	5.5e-06	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	170 - 316	5e-06	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	179 - 322	8.6e-06	Echinoderm microtubule-associated protein first beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0009337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27470 AT4G07410 AT4G07410
fragaria_vesca	FvH4_2g32880 FvH4_2g32880 FvH4_2g32880 FvH4_2g32880
malus_domestica	MD08G1080300.v1.1 MD15G1067400.v1.1
prunus_persica	Prupe.1G421300_v2.0.a1
pyrus_communis	pycom08g06630 pycom15g06390
rosa_chinensis	RchiOBHm_Chr6g0309831
rosa_laevigata	RLG00000010532
rosa_multiflora	Rmu_ssc0000373.1_g000003
rosa_roxburghii	Rroxscaffold_7G00159140
rosa_rugosa	Rorug06G0375100
rosa_samantha	Rh6AG488600 Rh6BG498100 Rh6CG502900 Rh6DG489000
rosa_wichuraiana	Rw6G042550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	957
AasI	GACNNNNNNGTC	1 cut(s)	83
AccB1I	GGYRCC	2 cut(s)	1409, 1925
AccB7I	CCANNNNNTGG	2 cut(s)	511, 1654
AccI	GTMKAC	2 cut(s)	72, 576
AccII	CGCG	1 cut(s)	1764
AciI	CCGC	2 cut(s)	90, 541
AclWI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
AcoI	YGGCCR	1 cut(s)	1645
AcsI	RAATTY	6 cut(s)	641, 705, 2072, 2081, 2245, 2332
AcuI	CTGAAG	1 cut(s)	27
AdeI	CACNNNGTG	2 cut(s)	1406, 1793
AfiI	CCNNNNNNNGG	7 cut(s)	33, 147, 511, 916, 1042, 1115, 1654
AflIII	ACRYGT	2 cut(s)	607, 2461
AgsI	TTSAA	7 cut(s)	298, 1319, 1576, 1914, 1992, 2215, 2245
AhdI	GACNNNNNGTC	1 cut(s)	627
AjiI	CACGTC	1 cut(s)	608
AjnI	CCWGG	4 cut(s)	99, 2076, 2086, 2153
AjuI	GAANNNNNNNTTGG	2 cut(s)	296, 328
AleI	CACNNNNGTG	1 cut(s)	876
Alw21I	GWGCWC	2 cut(s)	1603, 1746
Alw26I	GTCTC	4 cut(s)	133, 196, 334, 1438
Alw44I	GTGCAC	2 cut(s)	1599, 1742
AlwI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
AlwNI	CAGNNNCTG	2 cut(s)	1079, 1840
Ama87I	CYCGRG	3 cut(s)	113, 1883, 2353
AoxI	GGCC	4 cut(s)	697, 1033, 1444, 1645
ApaLI	GTGCAC	2 cut(s)	1599, 1742
ApeKI	GCWGC	7 cut(s)	92, 896, 899, 1506, 1844, 1897, 2047
ApoI	RAATTY	6 cut(s)	641, 705, 2072, 2081, 2245, 2332
ArsI	GACNNNNNNTTYG	2 cut(s)	738, 770
AseI	ATTAAT	1 cut(s)	480
AspA2I	CCTAGG	2 cut(s)	377, 593
AspLEI	GCGC	1 cut(s)	1511
AspS9I	GGNCC	4 cut(s)	382, 697, 746, 1444
AsuC2I	CCSGG	1 cut(s)	135
AsuHPI	GGTGA	6 cut(s)	185, 890, 1021, 1238, 1744, 1810
AsuII	TTCGAA	1 cut(s)	2384
AvaI	CYCGRG	3 cut(s)	113, 1883, 2353
AvaII	GGWCC	2 cut(s)	382, 746
AvrII	CCTAGG	2 cut(s)	377, 593
BaeGI	GKGCMC	3 cut(s)	1195, 1603, 1746
BaeI	ACNNNNGTAYC	2 cut(s)	851, 884
BalI	TGGCCA	1 cut(s)	1647
BanI	GGYRCC	2 cut(s)	1409, 1925
BanII	GRGCYC	1 cut(s)	2106
BauI	CACGAG	1 cut(s)	1739
BbsI	GAAGAC	2 cut(s)	311, 990
Bbv12I	GWGCWC	2 cut(s)	1603, 1746
BbvI	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BccI	CCATC	6 cut(s)	524, 932, 1417, 1823, 1856, 2413
BceAI	ACGGC	2 cut(s)	121, 2337
BcgI	CGANNNNNNTGC	2 cut(s)	211, 245
BciT130I	CCWGG	4 cut(s)	101, 2078, 2088, 2155
BcnI	CCSGG	1 cut(s)	135
BcoDI	GTCTC	4 cut(s)	133, 196, 334, 1438
BfaI	CTAG	9 cut(s)	59, 378, 594, 966, 984, 1371, 1518, 1560, 1791
BglI	GCCNNNNNGGC	1 cut(s)	105
BglII	AGATCT	1 cut(s)	1459
BisI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
BlnI	CCTAGG	2 cut(s)	377, 593
BlsI	GCNGC	8 cut(s)	91, 94, 898, 901, 1508, 1846, 1899, 2049
BmcAI	AGTACT	1 cut(s)	811
Bme1390I	CCNGG	5 cut(s)	101, 135, 2078, 2088, 2155
Bme18I	GGWCC	2 cut(s)	382, 746
BmeRI	GACNNNNNGTC	1 cut(s)	627
BmeT110I	CYCGRG	3 cut(s)	113, 1883, 2353
BmgBI	CACGTC	1 cut(s)	608
BmgT120I	GGNCC	4 cut(s)	382, 697, 746, 1444
BmrFI	CCNGG	5 cut(s)	101, 135, 2078, 2088, 2155
BmrI	ACTGGG	1 cut(s)	2039
BmsI	GCATC	4 cut(s)	267, 625, 676, 1496
BmuI	ACTGGG	1 cut(s)	2039
BpiI	GAAGAC	2 cut(s)	311, 990
BplI	GAGNNNNNCTC	2 cut(s)	1722, 1754
Bpu14I	TTCGAA	1 cut(s)	2384
BpuEI	CTTGAG	1 cut(s)	634
BpuMI	CCSGG	1 cut(s)	135
BsaAI	YACGTR	1 cut(s)	1397
BsaI	GGTCTC	1 cut(s)	334
BsaJI	CCNNGG	8 cut(s)	48, 133, 377, 423, 593, 1653, 2154, 2414
Bsc4I	CCNNNNNNNGG	7 cut(s)	33, 147, 511, 916, 1042, 1115, 1654
Bse1I	ACTGG	7 cut(s)	444, 817, 1326, 1821, 1907, 2034, 2042
Bse3DI	GCAATG	4 cut(s)	403, 587, 1349, 2166
BseBI	CCWGG	4 cut(s)	101, 2078, 2088, 2155
BseDI	CCNNGG	8 cut(s)	48, 133, 377, 423, 593, 1653, 2154, 2414
BseGI	GGATG	7 cut(s)	436, 675, 691, 1337, 1726, 2017, 2424
BseLI	CCNNNNNNNGG	7 cut(s)	33, 147, 511, 916, 1042, 1115, 1654
BseMI	GCAATG	4 cut(s)	403, 587, 1349, 2166
BseMII	CTCAG	3 cut(s)	465, 770, 996
BseNI	ACTGG	7 cut(s)	444, 817, 1326, 1821, 1907, 2034, 2042
BseRI	GAGGAG	1 cut(s)	2033
BseSI	GKGCMC	3 cut(s)	1195, 1603, 1746
BseXI	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BseYI	CCCAGC	1 cut(s)	905
BsgI	GTGCAG	3 cut(s)	822, 1281, 2430
Bsh1236I	CGCG	1 cut(s)	1764
BshFI	GGCC	4 cut(s)	699, 1035, 1446, 1647
BshNI	GGYRCC	2 cut(s)	1409, 1925
BsiHKAI	GWGCWC	2 cut(s)	1603, 1746
BsiHKCI	CYCGRG	3 cut(s)	113, 1883, 2353
BsiSI	CCGG	1 cut(s)	135
BslFI	GGGAC	2 cut(s)	395, 845
BslI	CCNNNNNNNGG	7 cut(s)	33, 147, 511, 916, 1042, 1115, 1654
BsmAI	GTCTC	4 cut(s)	133, 196, 334, 1438
BsmBI	CGTCTC	1 cut(s)	133
BsmFI	GGGAC	2 cut(s)	395, 845
BsnI	GGCC	4 cut(s)	699, 1035, 1446, 1647
Bso31I	GGTCTC	1 cut(s)	334
BsoBI	CYCGRG	3 cut(s)	113, 1883, 2353
Bsp119I	TTCGAA	1 cut(s)	2384
Bsp1286I	GDGCHC	4 cut(s)	1195, 1603, 1746, 2106
Bsp1407I	TGTACA	1 cut(s)	1360
Bsp19I	CCATGG	1 cut(s)	1653
Bsp68I	TCGCGA	1 cut(s)	1764
BspACI	CCGC	2 cut(s)	90, 541
BspANI	GGCC	4 cut(s)	699, 1035, 1446, 1647
BspCNI	CTCAG	3 cut(s)	466, 771, 997
BspFNI	CGCG	1 cut(s)	1764
BspPI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
BspT104I	TTCGAA	1 cut(s)	2384
BspT107I	GGYRCC	2 cut(s)	1409, 1925
BspTNI	GGTCTC	1 cut(s)	334
BsrDI	GCAATG	4 cut(s)	403, 587, 1349, 2166
BsrGI	TGTACA	1 cut(s)	1360
BsrI	ACTGG	7 cut(s)	444, 817, 1326, 1821, 1907, 2034, 2042
BssECI	CCNNGG	8 cut(s)	48, 133, 377, 423, 593, 1653, 2154, 2414
BssNAI	GTATAC	1 cut(s)	577
BssSI	CACGAG	1 cut(s)	1739
BssT1I	CCWWGG	4 cut(s)	48, 377, 593, 1653
Bst1107I	GTATAC	1 cut(s)	577
Bst2BI	CACGAG	1 cut(s)	1739
Bst2UI	CCWGG	4 cut(s)	101, 2078, 2088, 2155
Bst6I	CTCTTC	1 cut(s)	1993
BstAUI	TGTACA	1 cut(s)	1360
BstBAI	YACGTR	1 cut(s)	1397
BstBI	TTCGAA	1 cut(s)	2384
BstC8I	GCNNGC	5 cut(s)	97, 149, 217, 858, 1842
BstDEI	CTNAG	4 cut(s)	474, 779, 1005, 1419
BstDSI	CCRYGG	2 cut(s)	1653, 2414
BstENI	CCTNNNNNAGG	1 cut(s)	1040
BstF5I	GGATG	7 cut(s)	436, 675, 691, 1337, 1726, 2017, 2424
BstFNI	CGCG	1 cut(s)	1764
BstHHI	GCGC	1 cut(s)	1511
BstMAI	GTCTC	4 cut(s)	133, 196, 334, 1438
BstMWI	GCNNNNNNNGC	5 cut(s)	17, 105, 144, 157, 896
BstNI	CCWGG	4 cut(s)	101, 2078, 2088, 2155
BstNSI	RCATGY	1 cut(s)	2465
BstSCI	CCNGG	5 cut(s)	99, 133, 2076, 2086, 2153
BstSLI	GKGCMC	3 cut(s)	1195, 1603, 1746
BstSNI	TACGTA	1 cut(s)	1397
BstUI	CGCG	1 cut(s)	1764
BstV1I	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BstV2I	GAAGAC	2 cut(s)	311, 990
BstX2I	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
BstXI	CCANNNNNNTGG	1 cut(s)	392
BstYI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
BstZ17I	GTATAC	1 cut(s)	577
BsuRI	GGCC	4 cut(s)	699, 1035, 1446, 1647
BtgI	CCRYGG	2 cut(s)	1653, 2414
BtrI	CACGTC	1 cut(s)	608
BtsCI	GGATG	7 cut(s)	436, 675, 691, 1337, 1726, 2017, 2424
BtsI	GCAGTG	3 cut(s)	284, 823, 1841
BtuMI	TCGCGA	1 cut(s)	1764
Cac8I	GCNNGC	5 cut(s)	97, 149, 217, 858, 1842
CaiI	CAGNNNCTG	2 cut(s)	1079, 1840
CfoI	GCGC	1 cut(s)	1511
Cfr13I	GGNCC	4 cut(s)	382, 697, 746, 1444
CseI	GACGC	1 cut(s)	58
DdeI	CTNAG	4 cut(s)	474, 779, 1005, 1419
DraI	TTTAAA	1 cut(s)	1276
DraIII	CACNNNGTG	2 cut(s)	1406, 1793
DrdI	GACNNNNNNGTC	1 cut(s)	83
DriI	GACNNNNNGTC	1 cut(s)	627
DseDI	GACNNNNNNGTC	1 cut(s)	83
EaeI	YGGCCR	1 cut(s)	1645
Eam1104I	CTCTTC	1 cut(s)	1993
Eam1105I	GACNNNNNGTC	1 cut(s)	627
EarI	CTCTTC	1 cut(s)	1993
Eco105I	TACGTA	1 cut(s)	1397
Eco130I	CCWWGG	4 cut(s)	48, 377, 593, 1653
Eco24I	GRGCYC	1 cut(s)	2106
Eco31I	GGTCTC	1 cut(s)	334
Eco32I	GATATC	2 cut(s)	1288, 1384
Eco47I	GGWCC	2 cut(s)	382, 746
Eco57I	CTGAAG	1 cut(s)	27
Eco88I	CYCGRG	3 cut(s)	113, 1883, 2353
EcoNI	CCTNNNNNAGG	1 cut(s)	1040
EcoRII	CCWGG	4 cut(s)	99, 2076, 2086, 2153
EcoRV	GATATC	2 cut(s)	1288, 1384
EcoT14I	CCWWGG	4 cut(s)	48, 377, 593, 1653
EcoT38I	GRGCYC	1 cut(s)	2106
ErhI	CCWWGG	4 cut(s)	48, 377, 593, 1653
Esp3I	CGTCTC	1 cut(s)	133
FalI	AAGNNNNNCTT	2 cut(s)	2116, 2148
FaqI	GGGAC	2 cut(s)	395, 845
FblI	GTMKAC	2 cut(s)	72, 576
Fnu4HI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
FokI	GGATG	7 cut(s)	443, 682, 698, 1324, 1733, 2024, 2431
FriOI	GRGCYC	1 cut(s)	2106
Fsp4HI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
FspBI	CTAG	9 cut(s)	59, 378, 594, 966, 984, 1371, 1518, 1560, 1791
GlaI	GCGC	1 cut(s)	1510
GluI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
GsaI	CCCAGC	1 cut(s)	909
HaeIII	GGCC	4 cut(s)	699, 1035, 1446, 1647
HapII	CCGG	1 cut(s)	135
HgaI	GACGC	1 cut(s)	58
HhaI	GCGC	1 cut(s)	1511
Hin6I	GCGC	1 cut(s)	1509
HinP1I	GCGC	1 cut(s)	1509
HincII	GTYRAC	1 cut(s)	73
HindII	GTYRAC	1 cut(s)	73
HindIII	AAGCTT	2 cut(s)	9, 1365
HpaII	CCGG	1 cut(s)	135
HphI	GGTGA	6 cut(s)	185, 890, 1021, 1238, 1744, 1810
Hpy99I	CGWCG	7 cut(s)	32, 74, 77, 146, 199, 256, 1146
HpyAV	CCTTC	1 cut(s)	1109
HpyCH4IV	ACGT	5 cut(s)	607, 1141, 1154, 1396, 2284
HpyF10VI	GCNNNNNNNGC	5 cut(s)	17, 105, 144, 157, 896
HpyF3I	CTNAG	4 cut(s)	474, 779, 1005, 1419
HpySE526I	ACGT	5 cut(s)	607, 1141, 1154, 1396, 2284
HspAI	GCGC	1 cut(s)	1509
LmnI	GCTCC	4 cut(s)	113, 143, 907, 1432
Lsp1109I	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
LweI	GCATC	4 cut(s)	267, 625, 676, 1496
MaeI	CTAG	9 cut(s)	59, 378, 594, 966, 984, 1371, 1518, 1560, 1791
MaeII	ACGT	5 cut(s)	607, 1141, 1154, 1396, 2284
MaeIII	GTNAC	4 cut(s)	619, 768, 1009, 1933
MflI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
MhlI	GDGCHC	4 cut(s)	1195, 1603, 1746, 2106
MlsI	TGGCCA	1 cut(s)	1647
MluNI	TGGCCA	1 cut(s)	1647
MlyI	GAGTC	5 cut(s)	71, 198, 637, 1482, 2365
MmeI	TCCRAC	3 cut(s)	294, 1989, 2258
Mox20I	TGGCCA	1 cut(s)	1647
MscI	TGGCCA	1 cut(s)	1647
MslI	CAYNNNNRTG	5 cut(s)	876, 1053, 1200, 2001, 2460
Msp20I	TGGCCA	1 cut(s)	1647
MspA1I	CMGCKG	4 cut(s)	92, 160, 473, 1940
MspI	CCGG	1 cut(s)	135
MspR9I	CCNGG	5 cut(s)	101, 135, 2078, 2088, 2155
MssI	GTTTAAAC	1 cut(s)	1276
MvaI	CCWGG	4 cut(s)	101, 2078, 2088, 2155
MvnI	CGCG	1 cut(s)	1764
MwoI	GCNNNNNNNGC	5 cut(s)	17, 105, 144, 157, 896
NciI	CCSGG	1 cut(s)	135
NcoI	CCATGG	1 cut(s)	1653
NmuCI	GTSAC	3 cut(s)	619, 1009, 1933
NruI	TCGCGA	1 cut(s)	1764
NspI	RCATGY	1 cut(s)	2465
NspV	TTCGAA	1 cut(s)	2384
OliI	CACNNNNGTG	1 cut(s)	876
PaeR7I	CTCGAG	2 cut(s)	113, 1883
PciI	ACATGT	1 cut(s)	2461
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 191
PflMI	CCANNNNNTGG	2 cut(s)	511, 1654
PfoI	TCCNGGA	1 cut(s)	2076
PkrI	GCNGC	8 cut(s)	91, 94, 898, 901, 1508, 1846, 1899, 2049
PleI	GAGTC	5 cut(s)	71, 197, 636, 1481, 2364
PmeI	GTTTAAAC	1 cut(s)	1276
PpsI	GAGTC	5 cut(s)	71, 197, 636, 1481, 2364
Ppu21I	YACGTR	1 cut(s)	1397
PscI	ACATGT	1 cut(s)	2461
PshBI	ATTAAT	1 cut(s)	480
PsiI	TTATAA	1 cut(s)	957
Psp6I	CCWGG	4 cut(s)	99, 2076, 2086, 2153
PspFI	CCCAGC	1 cut(s)	905
PspGI	CCWGG	4 cut(s)	99, 2076, 2086, 2153
PspPI	GGNCC	4 cut(s)	382, 697, 746, 1444
PstNI	CAGNNNCTG	2 cut(s)	1079, 1840
PsuI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
PvuII	CAGCTG	3 cut(s)	160, 473, 1940
RruI	TCGCGA	1 cut(s)	1764
RseI	CAYNNNNRTG	5 cut(s)	876, 1053, 1200, 2001, 2460
SalI	GTCGAC	1 cut(s)	71
SatI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
Sau96I	GGNCC	4 cut(s)	382, 697, 746, 1444
ScaI	AGTACT	1 cut(s)	811
SchI	GAGTC	5 cut(s)	71, 198, 637, 1482, 2365
ScrFI	CCNGG	5 cut(s)	101, 135, 2078, 2088, 2155
SduI	GDGCHC	4 cut(s)	1195, 1603, 1746, 2106
SfaNI	GCATC	4 cut(s)	267, 625, 676, 1496
Sfr274I	CTCGAG	2 cut(s)	113, 1883
SfuI	TTCGAA	1 cut(s)	2384
SgrDI	CGTCGACG	1 cut(s)	71
SinI	GGWCC	2 cut(s)	382, 746
SlaI	CTCGAG	2 cut(s)	113, 1883
SmiMI	CAYNNNNRTG	5 cut(s)	876, 1053, 1200, 2001, 2460
SmlI	CTYRAG	3 cut(s)	113, 613, 1883
SmoI	CTYRAG	3 cut(s)	113, 613, 1883
SnaBI	TACGTA	1 cut(s)	1397
SsiI	CCGC	2 cut(s)	90, 541
SspI	AATATT	1 cut(s)	1165
SspMI	CTAG	9 cut(s)	59, 378, 594, 966, 984, 1371, 1518, 1560, 1791
StyD4I	CCNGG	5 cut(s)	99, 133, 2076, 2086, 2153
StyI	CCWWGG	4 cut(s)	48, 377, 593, 1653
TaiI	ACGT	5 cut(s)	610, 1144, 1157, 1399, 2287
TatI	WGTACW	3 cut(s)	359, 809, 1360
TauI	GCSGC	1 cut(s)	92
TseFI	GTSAC	3 cut(s)	619, 1009, 1933
TseI	GCWGC	7 cut(s)	92, 896, 899, 1506, 1844, 1897, 2047
Tsp45I	GTSAC	3 cut(s)	619, 1009, 1933
TspDTI	ATGAA	9 cut(s)	158, 381, 717, 1128, 1584, 1897, 2118, 2217, 2342
TspGWI	ACGGA	2 cut(s)	16, 130
Van91I	CCANNNNNTGG	2 cut(s)	511, 1654
VneI	GTGCAC	2 cut(s)	1599, 1742
VpaK11BI	GGWCC	2 cut(s)	382, 746
VspI	ATTAAT	1 cut(s)	480
XagI	CCTNNNNNAGG	1 cut(s)	1040
XapI	RAATTY	6 cut(s)	641, 705, 2072, 2081, 2245, 2332
XceI	RCATGY	1 cut(s)	2465
XhoI	CTCGAG	2 cut(s)	113, 1883
XmaJI	CCTAGG	2 cut(s)	377, 593
XmiI	GTMKAC	2 cut(s)	72, 576
XspI	CTAG	9 cut(s)	59, 378, 594, 966, 984, 1371, 1518, 1560, 1791
ZrmI	AGTACT	1 cut(s)	811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.