Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009337

Physical Location & Seq

Genomic Coordinates Forward (+)

4684079 .. 4689269

Gene Structure 5191 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1067400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGTCTGAAGCTTACAGGACCAGCTCCGTCGATTGGAAGCCCTCTCCCTTGGTTGCCCTAGCCACCAGCGTCGACGACTCCCAAGTCGCCGCTGCTCGCGAGGATGGCTCCCTCGAGATTTGGCTCGTCTCCCCCGGCTCCGTCGGCTGGCACTGTCAGCTGACGATTCATGGCGATCCTGAATCGAGAGTATCGTCGCTTATTTGGTGTCGTGCCGGCTCGAAAGGATTGCCTTGTGGACGGTTGTTTTCCTCGAGCATCAATGGTTCAGTTTTGCAGTGGGATCTTTTCCATTTGAAGCAGAAGACCGTGTTGGATTCTATTGGGGTCTCGATCTGGCAAATGGCTGTTGCACCATGTAGTAGTGATATAGAATCAAAGGACTACCCTATTGGAAATGGGTGTTTAAAAGCTAATAGTACCGAGGGGGGTGATCCAGAAACAAGCGATAGCGAAGATGATTCCGACTCCGCTGAGATTAATGAACAATCAGTTGTTGAGGACCAGCTTGTGGCATTAGCTTGTGATGATGGTTGTGTGCGAATATACAGTATTACTAAAACAGATGAGTTTGTTTACACTAGATCGTTGCCTAGGGTCAGTGGACGTGTCTTGAGTGTGACATGGAGTCCTGATACTAAATTTATATATTCGGGGAGTAGTGATGGGATGATAAGATGCTGGGATGCTAAATTGGGCCATGAAATCTATAGGATTACAGTTGGGCTTGGAGGTCTGGGAAGTGGACCTGAACTTTGTGTTTGGTCGTTACTTTCTCTGAGGTGTGGGAACCTTGTCAGTGCAGACAGTACTGGCAGTGTTCAGTTTTGGGACAGTCAGCATGGAACTCTATTGCAGGCACATTCGTACCACAAAGGTGATGTAAATGCTCTGGCAGCAGCCCCCAGCCATAATAGGGTGTTTTCCGCTGGTTCTGATGGTCAGGTTATTCTTTATAAGCTCTCTAGTGTGACGGTGGGATCTAGTGATGACAAGTCTTCTTCTGAGTTATTGAAGAAATGGATCTATGTTGGTCTTGTAAAAGCTCATACACACGATGTTAGGGCTTTGACAGTGGCTGTACCAATCAGCAGAGAAGATCCCTTGCCAGATGAAGGTATAAAAATACACCGTAAAGAACGTCGTAAGATAACGCGTAATAATATTAATGAGTTTAGTTATCGTAAATGGGCACATTTGGGTGTTCCCATGCTTATATCTGCTGGTGATGACACAAAGTTAATCGCATATCCTGTTAAGGAGTTCACACAGTTTAAACCTCATGATATCTGCCCTGCGCCTCAGAGACTACCAATTCAACTGGTGCTTAATAGAACATCCAAACAGTCATTGCTTCTTGTACAAGCTTCTAGTTGGGTGGATATTCTTCTTGTACGTGCACAAAGTGGCACCTTTTCCAAGATAGCTCCTGTCTCCAAATGGGGCCAAGCCTCAACAGATCTTTTGGTTCGAGTCAAGAGTAAGGCATATCGGAAGATCATTTGCAGCACAATTTCTAATACGGGAGTGCTTTTTGCATATTCTGATCATGTGAAACCTAGCCTATATGAACTGAAGAAGAATAAAGTTGGCAAAAGTGCACTGACTGTTAATAAAAGACAGCTTCCACAGAAACTACCATTTGCCCATTCCATGGTTTTTAGTTCTGATTCTTCTCGCTTGATGATAGCTGGGCATGATAGAAGAATATATGTTGTGGATGTGAGCAGAGAAGAACTAGTGCACAGGTTCACCCCTTGTCGCGATTTGCAGGATCAAGAATTACCACCTAGTGAGCCTCCCATAACAAAACTGTTCACCAGTTGTGATGGGCAGTGGTTGGCTGCCATCAACTGCTTTGGAGATATTTATGTATTTAATCTGGAGATACAAAGGCAGCACTGGTTCATTTCAAGACTAAATGGCGCATCTGTGACAGCTGGTGGTTTTTCTCCTCGAAACAACAATGTGCTTGTAATCACAACCTCTTCAAATCATGTATATGCATTGGATGTTGAGGAGAGAGAACTGGGAAACTGGTCGAGGCAGCATACGTATTGTCTACCAAAGAAATTCCAAGAATTTCCAGGCGAAGTTATTGGGCTCTCTTTCCCACCCTCCTCAAGTTCATCTGCTGTTATTGTTTACAGTGCCAGGGCAATGTGCTGGATTGATTTTGGGAAGCCCATTGATCCAGACGATGAAAGTGGCATGCCAAACGGTCAGAATCCGACATCGATGAATTTGCAAAGTAGTAATTCGATTAAGAAGATTCTAAAACGTAAATTGACAGACAGTGAAACTGAGAGTAGACTTATGGCTATGAAGAATTTTGATTTTATTTGGTTCCCAAGTCCAGCTTTATTTTTGGGTCATCTTTCGAATAGTTCTCTGTTGATGATAGACAAACCGTGGATGGAAGTGGTTAAATCGTTGGACTCTGCACCTGTACACAGACATGTTTACGGGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010015 GO:0010051 GO:0010073 GO:0010101 GO:0010102 GO:0010154 GO:0010305 GO:0010311 GO:0010449 GO:0010817 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0032879 GO:0035266 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0051049 GO:0061458 GO:0065007 GO:0065008 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393 GO:2000012

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

825

Amino Acids

91.12

Weight (kDa)

7.3

Isoelectric Point (pI)

42.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	19 - 234	4.1e-10	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	166 - 236	1.4e-07	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	167 - 321	2.2e-12	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	167 - 236	9.3e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	167 - 235	2.6e-06	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	167 - 236	9.9e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	167 - 328	2.6e-07	TEP-1 second beta-propeller
WDR55	PF24796	168 - 338	3e-08	WDR55
WD40_CDC20-Fz	PF24807	169 - 357	4.1e-10	CDC20/Fizzy WD40 domain
WD40	PF00400	202 - 229	5.2e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27470 AT4G07410 AT4G07410
fragaria_vesca	FvH4_2g32880 FvH4_2g32880 FvH4_2g32880 FvH4_2g32880
malus_domestica	MD08G1080300.v1.1 MD15G1067400.v1.1
prunus_persica	Prupe.1G421300_v2.0.a1
pyrus_communis	pycom08g06630 pycom15g06390
rosa_chinensis	RchiOBHm_Chr6g0309831
rosa_laevigata	RLG00000010532
rosa_multiflora	Rmu_ssc0000373.1_g000003
rosa_roxburghii	Rroxscaffold_7G00159140
rosa_rugosa	Rorug06G0375100
rosa_samantha	Rh6AG488600 Rh6BG498100 Rh6CG502900 Rh6DG489000
rosa_wichuraiana	Rw6G042550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	957
AasI	GACNNNNNNGTC	1 cut(s)	83
AccB1I	GGYRCC	1 cut(s)	1409
AccB7I	CCANNNNNTGG	2 cut(s)	511, 1654
AccI	GTMKAC	3 cut(s)	72, 2062, 2311
AccII	CGCG	3 cut(s)	99, 1156, 1764
AciI	CCGC	3 cut(s)	90, 471, 927
AclWI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
AcsI	RAATTY	5 cut(s)	641, 2072, 2081, 2242, 2329
AcuI	CTGAAG	2 cut(s)	27, 1595
AdeI	CACNNNGTG	2 cut(s)	1406, 1793
AfaI	GTAC	7 cut(s)	421, 811, 869, 1083, 1362, 1395, 2451
AfiI	CCNNNNNNNGG	6 cut(s)	33, 147, 511, 916, 1115, 1654
AflIII	ACRYGT	3 cut(s)	607, 1154, 2458
AgsI	TTSAA	5 cut(s)	298, 1015, 1319, 1914, 1992
AhdI	GACNNNNNGTC	1 cut(s)	627
AhlI	ACTAGT	1 cut(s)	1738
AjiI	CACGTC	1 cut(s)	608
AjnI	CCWGG	2 cut(s)	2086, 2153
AjuI	GAANNNNNNNTTGG	2 cut(s)	296, 328
AleI	CACNNNNGTG	1 cut(s)	876
Alw21I	GWGCWC	3 cut(s)	1402, 1603, 1746
Alw26I	GTCTC	4 cut(s)	133, 334, 1300, 1438
Alw44I	GTGCAC	3 cut(s)	1398, 1599, 1742
AlwI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
AlwNI	CAGNNNCTG	1 cut(s)	1079
Ama87I	CYCGRG	2 cut(s)	113, 253
AoxI	GGCC	2 cut(s)	697, 1444
ApaLI	GTGCAC	3 cut(s)	1398, 1599, 1742
ApeKI	GCWGC	7 cut(s)	92, 896, 899, 1506, 1844, 1897, 2047
ApoI	RAATTY	5 cut(s)	641, 2072, 2081, 2242, 2329
ArsI	GACNNNNNNTTYG	2 cut(s)	738, 770
AseI	ATTAAT	2 cut(s)	480, 1167
AspA2I	CCTAGG	1 cut(s)	593
AspLEI	GCGC	2 cut(s)	1300, 1928
AspS9I	GGNCC	5 cut(s)	18, 502, 697, 746, 1444
AsuC2I	CCSGG	1 cut(s)	135
AsuHPI	GGTGA	5 cut(s)	443, 890, 1238, 1744, 1810
AsuII	TTCGAA	1 cut(s)	2381
AvaI	CYCGRG	2 cut(s)	113, 253
AvaII	GGWCC	3 cut(s)	18, 502, 746
AvrII	CCTAGG	1 cut(s)	593
BaeGI	GKGCMC	4 cut(s)	1195, 1402, 1603, 1746
BaeI	ACNNNNGTAYC	2 cut(s)	851, 884
BanI	GGYRCC	1 cut(s)	1409
BanII	GRGCYC	1 cut(s)	2106
BbsI	GAAGAC	2 cut(s)	311, 990
Bbv12I	GWGCWC	3 cut(s)	1402, 1603, 1746
BbvI	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BccI	CCATC	7 cut(s)	98, 524, 659, 932, 1823, 1856, 2410
BcgI	CGANNNNNNTGC	4 cut(s)	211, 245, 2227, 2261
BciT130I	CCWGG	2 cut(s)	2088, 2155
BclI	TGATCA	1 cut(s)	1546
BcnI	CCSGG	1 cut(s)	135
BcoDI	GTCTC	4 cut(s)	133, 334, 1300, 1438
BcuI	ACTAGT	1 cut(s)	1738
BfaI	CTAG	9 cut(s)	59, 582, 594, 966, 984, 1371, 1560, 1739, 1791
BfmI	CTRYAG	1 cut(s)	709
BglII	AGATCT	1 cut(s)	1459
BisI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
BlnI	CCTAGG	1 cut(s)	593
BlsI	GCNGC	8 cut(s)	91, 94, 898, 901, 1508, 1846, 1899, 2049
BmcAI	AGTACT	1 cut(s)	811
Bme1390I	CCNGG	3 cut(s)	135, 2088, 2155
Bme18I	GGWCC	3 cut(s)	18, 502, 746
BmeRI	GACNNNNNGTC	1 cut(s)	627
BmeT110I	CYCGRG	2 cut(s)	113, 253
BmgBI	CACGTC	1 cut(s)	608
BmgT120I	GGNCC	5 cut(s)	18, 502, 697, 746, 1444
BmiI	GGNNCC	6 cut(s)	109, 139, 791, 1411, 1445, 2348
BmrFI	CCNGG	3 cut(s)	135, 2088, 2155
BmrI	ACTGGG	1 cut(s)	2039
BmsI	GCATC	4 cut(s)	267, 668, 676, 1937
BmuI	ACTGGG	1 cut(s)	2039
BpiI	GAAGAC	2 cut(s)	311, 990
BplI	GAGNNNNNCTC	2 cut(s)	92, 124
BpmI	CTGGAG	1 cut(s)	1904
Bpu14I	TTCGAA	1 cut(s)	2381
BpuEI	CTTGAG	2 cut(s)	634, 2107
BpuMI	CCSGG	1 cut(s)	135
Bsa29I	ATCGAT	1 cut(s)	2237
BsaAI	YACGTR	2 cut(s)	1397, 2055
BsaI	GGTCTC	1 cut(s)	334
BsaJI	CCNNGG	7 cut(s)	48, 133, 423, 593, 1653, 2154, 2411
Bsc4I	CCNNNNNNNGG	6 cut(s)	33, 147, 511, 916, 1115, 1654
Bse118I	RCCGGY	1 cut(s)	215
Bse1I	ACTGG	6 cut(s)	817, 1326, 1821, 1907, 2034, 2042
Bse3DI	GCAATG	2 cut(s)	1349, 2166
BseBI	CCWGG	2 cut(s)	2088, 2155
BseCI	ATCGAT	1 cut(s)	2237
BseDI	CCNNGG	7 cut(s)	48, 133, 423, 593, 1653, 2154, 2411
BseGI	GGATG	7 cut(s)	109, 675, 691, 1337, 1726, 2017, 2421
BseLI	CCNNNNNNNGG	6 cut(s)	33, 147, 511, 916, 1115, 1654
BseMI	GCAATG	2 cut(s)	1349, 2166
BseMII	CTCAG	5 cut(s)	465, 770, 996, 1316, 2295
BseNI	ACTGG	6 cut(s)	817, 1326, 1821, 1907, 2034, 2042
BseRI	GAGGAG	3 cut(s)	1944, 2033, 2110
BseSI	GKGCMC	4 cut(s)	1195, 1402, 1603, 1746
BseXI	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BseYI	CCCAGC	3 cut(s)	681, 905, 1691
BsgI	GTGCAG	2 cut(s)	822, 2427
Bsh1236I	CGCG	3 cut(s)	99, 1156, 1764
BshFI	GGCC	2 cut(s)	699, 1446
BshNI	GGYRCC	1 cut(s)	1409
BshVI	ATCGAT	1 cut(s)	2237
BsiHKAI	GWGCWC	3 cut(s)	1402, 1603, 1746
BsiHKCI	CYCGRG	2 cut(s)	113, 253
BsiSI	CCGG	2 cut(s)	135, 216
BslFI	GGGAC	1 cut(s)	845
BslI	CCNNNNNNNGG	6 cut(s)	33, 147, 511, 916, 1115, 1654
BsmAI	GTCTC	4 cut(s)	133, 334, 1300, 1438
BsmBI	CGTCTC	1 cut(s)	133
BsmFI	GGGAC	1 cut(s)	845
BsnI	GGCC	2 cut(s)	699, 1446
Bso31I	GGTCTC	1 cut(s)	334
BsoBI	CYCGRG	2 cut(s)	113, 253
Bsp119I	TTCGAA	1 cut(s)	2381
Bsp1286I	GDGCHC	5 cut(s)	1195, 1402, 1603, 1746, 2106
Bsp1407I	TGTACA	2 cut(s)	1360, 2449
Bsp19I	CCATGG	1 cut(s)	1653
Bsp68I	TCGCGA	2 cut(s)	99, 1764
BspACI	CCGC	3 cut(s)	90, 471, 927
BspANI	GGCC	2 cut(s)	699, 1446
BspCNI	CTCAG	5 cut(s)	466, 771, 997, 1315, 2296
BspDI	ATCGAT	1 cut(s)	2237
BspFNI	CGCG	3 cut(s)	99, 1156, 1764
BspHI	TCATGA	1 cut(s)	1282
BspLI	GGNNCC	6 cut(s)	109, 139, 791, 1411, 1445, 2348
BspPI	GGATC	8 cut(s)	170, 291, 428, 988, 1031, 1094, 1782, 2186
BspT104I	TTCGAA	1 cut(s)	2381
BspT107I	GGYRCC	1 cut(s)	1409
BspTNI	GGTCTC	1 cut(s)	334
BsrDI	GCAATG	2 cut(s)	1349, 2166
BsrFI	RCCGGY	1 cut(s)	215
BsrGI	TGTACA	2 cut(s)	1360, 2449
BsrI	ACTGG	6 cut(s)	817, 1326, 1821, 1907, 2034, 2042
BssAI	RCCGGY	1 cut(s)	215
BssECI	CCNNGG	7 cut(s)	48, 133, 423, 593, 1653, 2154, 2411
BssT1I	CCWWGG	3 cut(s)	48, 593, 1653
Bst2UI	CCWGG	2 cut(s)	2088, 2155
Bst6I	CTCTTC	1 cut(s)	1993
BstAUI	TGTACA	2 cut(s)	1360, 2449
BstBAI	YACGTR	2 cut(s)	1397, 2055
BstBI	TTCGAA	1 cut(s)	2381
BstC8I	GCNNGC	5 cut(s)	97, 149, 217, 858, 2213
BstDEI	CTNAG	5 cut(s)	474, 779, 1005, 1302, 2304
BstDSI	CCRYGG	2 cut(s)	1653, 2411
BstF5I	GGATG	7 cut(s)	109, 675, 691, 1337, 1726, 2017, 2421
BstFNI	CGCG	3 cut(s)	99, 1156, 1764
BstHHI	GCGC	2 cut(s)	1300, 1928
BstMAI	GTCTC	4 cut(s)	133, 334, 1300, 1438
BstMWI	GCNNNNNNNGC	4 cut(s)	105, 144, 157, 896
BstNI	CCWGG	2 cut(s)	2088, 2155
BstNSI	RCATGY	2 cut(s)	2215, 2462
BstSCI	CCNGG	3 cut(s)	133, 2086, 2153
BstSFI	CTRYAG	1 cut(s)	709
BstSLI	GKGCMC	4 cut(s)	1195, 1402, 1603, 1746
BstSNI	TACGTA	1 cut(s)	2055
BstUI	CGCG	3 cut(s)	99, 1156, 1764
BstV1I	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
BstV2I	GAAGAC	2 cut(s)	311, 990
BstX2I	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
BstYI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
Bsu15I	ATCGAT	1 cut(s)	2237
BsuRI	GGCC	2 cut(s)	699, 1446
BsuTUI	ATCGAT	1 cut(s)	2237
BtgI	CCRYGG	2 cut(s)	1653, 2411
BtrI	CACGTC	1 cut(s)	608
BtsCI	GGATG	7 cut(s)	109, 675, 691, 1337, 1726, 2017, 2421
BtsI	GCAGTG	3 cut(s)	284, 823, 1841
BtuMI	TCGCGA	2 cut(s)	99, 1764
Cac8I	GCNNGC	5 cut(s)	97, 149, 217, 858, 2213
CaiI	CAGNNNCTG	1 cut(s)	1079
CciI	TCATGA	1 cut(s)	1282
CfoI	GCGC	2 cut(s)	1300, 1928
Cfr10I	RCCGGY	1 cut(s)	215
Cfr13I	GGNCC	5 cut(s)	18, 502, 697, 746, 1444
ClaI	ATCGAT	1 cut(s)	2237
CseI	GACGC	1 cut(s)	58
Csp6I	GTAC	7 cut(s)	420, 810, 868, 1082, 1361, 1394, 2450
CviQI	GTAC	7 cut(s)	420, 810, 868, 1082, 1361, 1394, 2450
DdeI	CTNAG	5 cut(s)	474, 779, 1005, 1302, 2304
DraI	TTTAAA	2 cut(s)	408, 1276
DraIII	CACNNNGTG	2 cut(s)	1406, 1793
DrdI	GACNNNNNNGTC	1 cut(s)	83
DriI	GACNNNNNGTC	1 cut(s)	627
DseDI	GACNNNNNNGTC	1 cut(s)	83
Eam1104I	CTCTTC	1 cut(s)	1993
Eam1105I	GACNNNNNGTC	1 cut(s)	627
EarI	CTCTTC	1 cut(s)	1993
Eco105I	TACGTA	1 cut(s)	2055
Eco130I	CCWWGG	3 cut(s)	48, 593, 1653
Eco24I	GRGCYC	1 cut(s)	2106
Eco31I	GGTCTC	1 cut(s)	334
Eco32I	GATATC	1 cut(s)	1288
Eco47I	GGWCC	3 cut(s)	18, 502, 746
Eco57I	CTGAAG	2 cut(s)	27, 1595
Eco88I	CYCGRG	2 cut(s)	113, 253
EcoRII	CCWGG	2 cut(s)	2086, 2153
EcoRV	GATATC	1 cut(s)	1288
EcoT14I	CCWWGG	3 cut(s)	48, 593, 1653
EcoT22I	ATGCAT	1 cut(s)	2008
EcoT38I	GRGCYC	1 cut(s)	2106
ErhI	CCWWGG	3 cut(s)	48, 593, 1653
Esp3I	CGTCTC	1 cut(s)	133
FalI	AAGNNNNNCTT	2 cut(s)	2344, 2376
FaqI	GGGAC	1 cut(s)	845
FbaI	TGATCA	1 cut(s)	1546
FblI	GTMKAC	3 cut(s)	72, 2062, 2311
Fnu4HI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
FokI	GGATG	7 cut(s)	116, 682, 698, 1324, 1733, 2024, 2428
FriOI	GRGCYC	1 cut(s)	2106
Fsp4HI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
FspBI	CTAG	9 cut(s)	59, 582, 594, 966, 984, 1371, 1560, 1739, 1791
GlaI	GCGC	2 cut(s)	1299, 1927
GluI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
GsaI	CCCAGC	3 cut(s)	685, 909, 1695
GsuI	CTGGAG	1 cut(s)	1904
HaeIII	GGCC	2 cut(s)	699, 1446
HapII	CCGG	2 cut(s)	135, 216
HgaI	GACGC	1 cut(s)	58
HhaI	GCGC	2 cut(s)	1300, 1928
Hin6I	GCGC	2 cut(s)	1298, 1926
HinP1I	GCGC	2 cut(s)	1298, 1926
HincII	GTYRAC	1 cut(s)	73
HindII	GTYRAC	1 cut(s)	73
HindIII	AAGCTT	2 cut(s)	9, 1365
HpaII	CCGG	2 cut(s)	135, 216
HphI	GGTGA	5 cut(s)	443, 890, 1238, 1744, 1810
Hpy99I	CGWCG	6 cut(s)	32, 74, 77, 146, 199, 1146
HpyAV	CCTTC	1 cut(s)	1109
HpyCH4IV	ACGT	5 cut(s)	607, 1141, 1396, 2054, 2281
HpyF10VI	GCNNNNNNNGC	4 cut(s)	105, 144, 157, 896
HpyF3I	CTNAG	5 cut(s)	474, 779, 1005, 1302, 2304
HpySE526I	ACGT	5 cut(s)	607, 1141, 1396, 2054, 2281
HspAI	GCGC	2 cut(s)	1298, 1926
KroI	GCCGGC	1 cut(s)	215
KroNI	GCCGGC	1 cut(s)	217
Ksp22I	TGATCA	1 cut(s)	1546
LmnI	GCTCC	4 cut(s)	29, 113, 143, 1432
Lsp1109I	GCAGC	7 cut(s)	79, 908, 911, 1518, 1831, 1909, 2059
LweI	GCATC	4 cut(s)	267, 668, 676, 1937
MaeI	CTAG	9 cut(s)	59, 582, 594, 966, 984, 1371, 1560, 1739, 1791
MaeII	ACGT	5 cut(s)	607, 1141, 1396, 2054, 2281
MaeIII	GTNAC	4 cut(s)	619, 768, 970, 1933
MflI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
MhlI	GDGCHC	5 cut(s)	1195, 1402, 1603, 1746, 2106
MluI	ACGCGT	1 cut(s)	1154
MlyI	GAGTC	5 cut(s)	71, 461, 637, 1482, 2432
MmeI	TCCRAC	4 cut(s)	294, 489, 2255, 2415
Mph1103I	ATGCAT	1 cut(s)	2008
MroNI	GCCGGC	1 cut(s)	215
MslI	CAYNNNNRTG	4 cut(s)	876, 1200, 2001, 2457
MspA1I	CMGCKG	5 cut(s)	92, 160, 473, 929, 1940
MspI	CCGG	2 cut(s)	135, 216
MspR9I	CCNGG	3 cut(s)	135, 2088, 2155
MssI	GTTTAAAC	1 cut(s)	1276
MvaI	CCWGG	2 cut(s)	2088, 2155
MvnI	CGCG	3 cut(s)	99, 1156, 1764
MwoI	GCNNNNNNNGC	4 cut(s)	105, 144, 157, 896
NaeI	GCCGGC	1 cut(s)	217
NciI	CCSGG	1 cut(s)	135
NcoI	CCATGG	1 cut(s)	1653
NgoMIV	GCCGGC	1 cut(s)	215
NlaIV	GGNNCC	6 cut(s)	109, 139, 791, 1411, 1445, 2348
NmuCI	GTSAC	3 cut(s)	619, 970, 1933
NruI	TCGCGA	2 cut(s)	99, 1764
NsiI	ATGCAT	1 cut(s)	2008
NspI	RCATGY	2 cut(s)	2215, 2462
NspV	TTCGAA	1 cut(s)	2381
OliI	CACNNNNGTG	1 cut(s)	876
PaeI	GCATGC	1 cut(s)	2215
PaeR7I	CTCGAG	2 cut(s)	113, 253
PagI	TCATGA	1 cut(s)	1282
PciI	ACATGT	1 cut(s)	2458
PcsI	WCGNNNNNNNCGW	1 cut(s)	191
PdiI	GCCGGC	1 cut(s)	217
PfeI	GAWTC	8 cut(s)	166, 182, 317, 374, 461, 1670, 2227, 2272
PflMI	CCANNNNNTGG	2 cut(s)	511, 1654
PkrI	GCNGC	8 cut(s)	91, 94, 898, 901, 1508, 1846, 1899, 2049
PleI	GAGTC	5 cut(s)	71, 461, 636, 1481, 2432
PmeI	GTTTAAAC	1 cut(s)	1276
PpsI	GAGTC	5 cut(s)	71, 461, 636, 1481, 2432
Ppu21I	YACGTR	2 cut(s)	1397, 2055
PscI	ACATGT	1 cut(s)	2458
PshBI	ATTAAT	2 cut(s)	480, 1167
PsiI	TTATAA	1 cut(s)	957
Psp6I	CCWGG	2 cut(s)	2086, 2153
PspFI	CCCAGC	3 cut(s)	681, 905, 1691
PspGI	CCWGG	2 cut(s)	2086, 2153
PspN4I	GGNNCC	6 cut(s)	109, 139, 791, 1411, 1445, 2348
PspPI	GGNCC	5 cut(s)	18, 502, 697, 746, 1444
PspXI	VCTCGAGB	1 cut(s)	253
PstNI	CAGNNNCTG	1 cut(s)	1079
PsuI	RGATCY	5 cut(s)	283, 980, 1023, 1099, 1459
PvuII	CAGCTG	2 cut(s)	160, 1940
RruI	TCGCGA	2 cut(s)	99, 1764
RsaI	GTAC	7 cut(s)	421, 811, 869, 1083, 1362, 1395, 2451
RsaNI	GTAC	7 cut(s)	420, 810, 868, 1082, 1361, 1394, 2450
RseI	CAYNNNNRTG	4 cut(s)	876, 1200, 2001, 2457
SalI	GTCGAC	1 cut(s)	71
SatI	GCNGC	8 cut(s)	90, 93, 897, 900, 1507, 1845, 1898, 2048
Sau96I	GGNCC	5 cut(s)	18, 502, 697, 746, 1444
ScaI	AGTACT	1 cut(s)	811
SchI	GAGTC	5 cut(s)	71, 461, 637, 1482, 2432
ScrFI	CCNGG	3 cut(s)	135, 2088, 2155
SduI	GDGCHC	5 cut(s)	1195, 1402, 1603, 1746, 2106
SfaNI	GCATC	4 cut(s)	267, 668, 676, 1937
SfcI	CTRYAG	1 cut(s)	709
Sfr274I	CTCGAG	2 cut(s)	113, 253
SfuI	TTCGAA	1 cut(s)	2381
SgrDI	CGTCGACG	1 cut(s)	71
SinI	GGWCC	3 cut(s)	18, 502, 746
SlaI	CTCGAG	2 cut(s)	113, 253
SmiMI	CAYNNNNRTG	4 cut(s)	876, 1200, 2001, 2457
SmlI	CTYRAG	4 cut(s)	113, 253, 613, 2122
SmoI	CTYRAG	4 cut(s)	113, 253, 613, 2122
SnaBI	TACGTA	1 cut(s)	2055
SpeI	ACTAGT	1 cut(s)	1738
SphI	GCATGC	1 cut(s)	2215
SsiI	CCGC	3 cut(s)	90, 471, 927
SspI	AATATT	1 cut(s)	1165
SspMI	CTAG	9 cut(s)	59, 582, 594, 966, 984, 1371, 1560, 1739, 1791
StyD4I	CCNGG	3 cut(s)	133, 2086, 2153
StyI	CCWWGG	3 cut(s)	48, 593, 1653
TaiI	ACGT	5 cut(s)	610, 1144, 1399, 2057, 2284
TatI	WGTACW	3 cut(s)	809, 1360, 2449
TauI	GCSGC	1 cut(s)	92
TfiI	GAWTC	8 cut(s)	166, 182, 317, 374, 461, 1670, 2227, 2272
TseFI	GTSAC	3 cut(s)	619, 970, 1933
TseI	GCWGC	7 cut(s)	92, 896, 899, 1506, 1844, 1897, 2047
Tsp45I	GTSAC	3 cut(s)	619, 970, 1933
TspGWI	ACGGA	2 cut(s)	16, 130
Van91I	CCANNNNNTGG	2 cut(s)	511, 1654
VneI	GTGCAC	3 cut(s)	1398, 1599, 1742
VpaK11BI	GGWCC	3 cut(s)	18, 502, 746
VspI	ATTAAT	2 cut(s)	480, 1167
XapI	RAATTY	5 cut(s)	641, 2072, 2081, 2242, 2329
XceI	RCATGY	2 cut(s)	2215, 2462
XhoI	CTCGAG	2 cut(s)	113, 253
XmaJI	CCTAGG	1 cut(s)	593
XmiI	GTMKAC	3 cut(s)	72, 2062, 2311
XspI	CTAG	9 cut(s)	59, 582, 594, 966, 984, 1371, 1560, 1739, 1791
ZrmI	AGTACT	1 cut(s)	811
Zsp2I	ATGCAT	1 cut(s)	2008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.