Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0009337

Physical Location & Seq

Genomic Coordinates Forward (+)

2451091 .. 2456208

Gene Structure 5118 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010532

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGCTCGAAGTCTATAGGAACAGCTCCGTCGATTGGAAGCCGTCGCCGGTCGTTGCCCTAGCCACCAGCGTCGACGACTCGCAGGTCGCCGCCGCCCGCGCCGACGGCTCCGTCGAGATTTGGCTCGTCTCCCCCGGCGCCGTCGGATGGCACTGTCAGCTGACGATTCATGGAGACCCTAACACGAGCGTTTCGTCGCTCATTTGGTGCCGGGCTGGCTCGAAAGGCTTGCCTTGTGGGCGGTTGTTCTCTTCTAGCCTCGACGGCTCGGTTTCACATTGGGATCTTTTTAATTTGAAGCAGACGACTGTTTTGGATTCTGTGGGGGTCTCAATCTTTAATATGGCCATGGCACCCTGTGTTAATGATACAGAGAATGGGTTTTTGAATGATAATGGGGGTGATCCTGAAACCAGTGATAGTGAAGCCAGTGACAGCGAGGATGATTCCGACTCAGACGATGATCATGCACCATCAGTTCTTGTGAACCCGCGCGTGGCATTAGCTGGTGGTGATGGTTTTCTGCAAATTTACAGTATTAATGATGCAGATGAGTTTGTATACACTAAATCGCTGCCTAGGGTCAATGGTAGTGTTTTGAGTGTGGCGTGGAGTCCCGATGCAAAGTTTATATATGCAGGGAGTACTGATGGCGTGATAAGGATCTGGGATGCTAGAGGAGGCATGGAAAAACATAGGATTACAGTTGGACTCGGAGGCTTGGGTAGCGGATCTGAACTCTGTGTGTGGGCGTTACTTTCTCTGAGGTGTGGGACCCTTGTAAGTGCAGACAGTACTGGAAGTGTTCAGTTTTGGGACAGTCAGCATGGAACTCTACTGCAAGCACATTCATATCACAAGGGTGACGCGAAAGCTCTGGCAGCTGCTCCCAGCCATAATCGGGTGTTCTCTGCTGGTTCTGATGGTCAGGTTATTCTTTACAAGCTCTCTAGTGAGACGCTGGGATCTAGTGTTGGGTCTTCTCCTAATGTAATGAAGAAATGGATCTACGTTGGTTATACAAGAGCTCATACACATGATGTTAGGGCTTTGGCAGTGGCTGTACCAATCAGCAGAGAAGATCCCTTACCAGATCAAGGTAGAAAAAGAGCCCGTCGTAAGGACAAGCCTGTTGATTTTGGTTATCTTAAATGGGCACATTTGGGTGTTCCTATGCTTATCTCTGCTGGTGATGACACAAAGCTCTTTACCTACCCTGTTAAGGAGTTCACGCAGTTTTCTCCGCATGATATCTGCCCTGCGCCTCAGAGAGTATCCATTCAATTGGCGCCTAATACAGAGTTCAGTCAAACATCATTGCTTCTTGTTCAAGGTTCTCATTTGTTGGATATCTTGCGCGTACGAGTAAAAGCTGGTGCCTTTTTTGATATGGCTCGTGGCTCTTCAGGAGGCCTAGCCTCAACTAATGGGCTGTTTCAAATTAAGTCATCTCGGAAGATCATTTGCAGCACCATATCTAATTCAGGGGTGTTTTTTGCTTATTCTGACCATGTGAAAATTAGCCTGTTTGAGTTGAAGAAGTGTATTCGATCAGGAAAAAAAGAAGTGAAGTGTAAAGTTGGCGAAAGTGAATTGACTCTTAATAAAAGAGAACTTCCTCGAAAACTACCATTTGCTCATTCGATGGTTTTTAGTTTTGATTCTTCTCGCTTGATGATAGCAGGGCATGATAGAAGAATATATGTTGTGGATGTACACAGCTCAGAGCTAGTGCATGCTTTTTCCCCTTGTCGTGATTTGCATGATCAAGAACTACCACCTGGTGAGCCTCCCATAACAAAAATGTTTACGAGTTCTGATGGGCAGTGGTTGGCTGCCATCAACTGCTTTGGAGATGTATATGTATTTAATCTGGAGATACAGAGATTCCAAGAATTTCCAGGCGAAGTTATTGGGCTCTCTTTCCCACCTTCTCCTAGTACAACATCTGTTATTGTCTATAGTGCCAGGGCTATGTGCTTGATTGACTTTGGGATGCCTGTTGATCGAGACGATGAAAGTGACATGATAAATGGTCAGGATCCAGCATTGAGGAATTTCCAAAGTAATGGGAAATTAAAACGCAAGTGGACAGACAGTCAAACTAAGAGTAAACTCTTTGGTAGGAAGAATTTTGAATTTATTCCATTCGGAGATCCAGTTTTATTTATAGGACATCTTTCCAAAAGTTCCGTGTTGAAAATATACAAACCATGGATGGAAGTGGTTAGAAATTTCGACTCTGCACCTGTAGACAAACATACTTTTGGGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010015 GO:0010051 GO:0010073 GO:0010101 GO:0010102 GO:0010154 GO:0010305 GO:0010311 GO:0010449 GO:0010817 GO:0022414 GO:0022622 GO:0032501 GO:0032502 GO:0032879 GO:0035266 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0051049 GO:0061458 GO:0065007 GO:0065008 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1905392 GO:1905393 GO:2000012

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

WD40

Protein Analysis

759

Amino Acids

82.9

Weight (kDa)

6.35

Isoelectric Point (pI)

35.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	166 - 320	6.4e-11	WDR5 beta-propeller domain
WDR55	PF24796	166 - 320	7.8e-06	WDR55
Beta-prop_THOC3	PF25174	167 - 318	7.7e-11	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	182 - 317	7.7e-06	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	191 - 352	4.7e-09	CDC20/Fizzy WD40 domain
WD40	PF00400	197 - 224	2.2e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27470 AT4G07410 AT4G07410
fragaria_vesca	FvH4_2g32880 FvH4_2g32880 FvH4_2g32880 FvH4_2g32880
malus_domestica	MD08G1080300.v1.1 MD15G1067400.v1.1
prunus_persica	Prupe.1G421300_v2.0.a1
pyrus_communis	pycom08g06630 pycom15g06390
rosa_chinensis	RchiOBHm_Chr6g0309831
rosa_laevigata	RLG00000010532
rosa_multiflora	Rmu_ssc0000373.1_g000003
rosa_roxburghii	Rroxscaffold_7G00159140
rosa_rugosa	Rorug06G0375100
rosa_samantha	Rh6AG488600 Rh6BG498100 Rh6CG502900 Rh6DG489000
rosa_wichuraiana	Rw6G042550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	83, 110
Acc36I	ACCTGC	1 cut(s)	73
AccB1I	GGYRCC	5 cut(s)	137, 207, 352, 1288, 1376
AccI	GTMKAC	3 cut(s)	72, 561, 2253
AccII	CGCG	5 cut(s)	99, 493, 495, 869, 1359
AciI	CCGC	7 cut(s)	90, 93, 97, 241, 491, 729, 1244
AcoI	YGGCCR	1 cut(s)	345
AcsI	RAATTY	6 cut(s)	528, 1895, 2056, 2131, 2138, 2233
AcuI	CTGAAG	1 cut(s)	1389
AcyI	GRCGYC	2 cut(s)	138, 1289
AdeI	CACNNNGTG	2 cut(s)	359, 1784
AfaI	GTAC	6 cut(s)	646, 796, 1065, 1362, 1716, 1942
AfiI	CCNNNNNNNGG	7 cut(s)	33, 147, 496, 901, 1097, 1120, 1222
AgsI	TTSAA	8 cut(s)	298, 388, 1283, 1331, 1439, 1537, 2138, 2200
AhdI	GACNNNNNGTC	1 cut(s)	2097
AjnI	CCWGG	3 cut(s)	1780, 1900, 1967
AleI	CACNNNNGTG	1 cut(s)	861
Alw21I	GWGCWC	1 cut(s)	1030
Alw26I	GTCTC	5 cut(s)	133, 168, 334, 950, 2004
AlwNI	CAGNNNCTG	1 cut(s)	1061
AoxI	GGCC	2 cut(s)	345, 1411
ApeKI	GCWGC	5 cut(s)	574, 881, 884, 1467, 1835
ApoI	RAATTY	6 cut(s)	528, 1895, 2056, 2131, 2138, 2233
ArsI	GACNNNNNNTTYG	2 cut(s)	295, 327
AseI	ATTAAT	1 cut(s)	540
Asp700I	GAANNNNTTC	1 cut(s)	2142
AspA2I	CCTAGG	1 cut(s)	578
AspLEI	GCGC	6 cut(s)	101, 140, 495, 1264, 1291, 1359
AspS9I	GGNCC	1 cut(s)	774
AsuC2I	CCSGG	2 cut(s)	135, 212
AsuHPI	GGTGA	5 cut(s)	413, 524, 875, 1202, 1796
AvaII	GGWCC	1 cut(s)	774
AvrII	CCTAGG	1 cut(s)	578
BaeGI	GKGCMC	1 cut(s)	1159
BalI	TGGCCA	1 cut(s)	347
BamHI	GGATCC	1 cut(s)	2041
BanI	GGYRCC	5 cut(s)	137, 207, 352, 1288, 1376
BanII	GRGCYC	3 cut(s)	1030, 1114, 1920
BarI	GAAGNNNNNNTAC	2 cut(s)	1071, 1103
BauI	CACGAG	2 cut(s)	184, 1395
BbsI	GAAGAC	1 cut(s)	972
Bbv12I	GWGCWC	1 cut(s)	1030
BbvI	GCAGC	5 cut(s)	561, 871, 893, 1479, 1822
BccI	CCATC	9 cut(s)	141, 481, 509, 644, 917, 1639, 1814, 1847, 2212
BceAI	ACGGC	4 cut(s)	25, 121, 125, 280
BcgI	CGANNNNNNTGC	4 cut(s)	211, 245, 449, 483
BciT130I	CCWGG	3 cut(s)	1782, 1902, 1969
BciVI	GTATCC	1 cut(s)	1285
BclI	TGATCA	2 cut(s)	463, 1765
BcnI	CCSGG	2 cut(s)	135, 212
BcoDI	GTCTC	5 cut(s)	133, 168, 334, 950, 2004
BfaI	CTAG	9 cut(s)	59, 255, 579, 675, 951, 969, 1415, 1730, 1938
BfmI	CTRYAG	3 cut(s)	13, 1960, 2250
BfoI	RGCGCY	2 cut(s)	141, 1292
BfuAI	ACCTGC	1 cut(s)	73
BfuI	GTATCC	1 cut(s)	1285
BglI	GCCNNNNNGGC	3 cut(s)	216, 238, 264
BisI	GCNGC	7 cut(s)	90, 93, 575, 882, 885, 1468, 1836
BlnI	CCTAGG	1 cut(s)	578
BlsI	GCNGC	7 cut(s)	91, 94, 576, 883, 886, 1469, 1837
BmcAI	AGTACT	2 cut(s)	646, 796
Bme1390I	CCNGG	5 cut(s)	135, 212, 1782, 1902, 1969
Bme18I	GGWCC	1 cut(s)	774
BmeRI	GACNNNNNGTC	1 cut(s)	2097
BmgT120I	GGNCC	1 cut(s)	774
BmiI	GGNNCC	9 cut(s)	109, 139, 209, 354, 775, 776, 1290, 1378, 2043
BmrFI	CCNGG	5 cut(s)	135, 212, 1782, 1902, 1969
BmsI	GCATC	4 cut(s)	535, 610, 661, 1986
BpiI	GAAGAC	1 cut(s)	972
BpmI	CTGGAG	1 cut(s)	1895
BpuMI	CCSGG	2 cut(s)	135, 212
BsaBI	GATNNNNATC	1 cut(s)	662
BsaHI	GRCGYC	2 cut(s)	138, 1289
BsaI	GGTCTC	2 cut(s)	168, 334
BsaJI	CCNNGG	5 cut(s)	133, 348, 578, 1968, 2213
BsaXI	ACNNNNNCTCC	2 cut(s)	2145, 2175
Bsc4I	CCNNNNNNNGG	7 cut(s)	33, 147, 496, 901, 1097, 1120, 1222
Bse118I	RCCGGY	1 cut(s)	46
Bse1I	ACTGG	4 cut(s)	414, 429, 802, 2159
Bse3DI	GCAATG	1 cut(s)	1316
Bse8I	GATNNNNATC	1 cut(s)	662
BseBI	CCWGG	3 cut(s)	1782, 1902, 1969
BseDI	CCNNGG	5 cut(s)	133, 348, 578, 1968, 2213
BseGI	GGATG	6 cut(s)	152, 448, 676, 1717, 2001, 2223
BseJI	GATNNNNATC	1 cut(s)	662
BseLI	CCNNNNNNNGG	7 cut(s)	33, 147, 496, 901, 1097, 1120, 1222
BseMI	GCAATG	1 cut(s)	1316
BseMII	CTCAG	4 cut(s)	468, 755, 1280, 1737
BseNI	ACTGG	4 cut(s)	414, 429, 802, 2159
BseRI	GAGGAG	1 cut(s)	693
BseSI	GKGCMC	1 cut(s)	1159
BseXI	GCAGC	5 cut(s)	561, 871, 893, 1479, 1822
BseYI	CCCAGC	2 cut(s)	890, 961
BsgI	GTGCAG	2 cut(s)	807, 2229
Bsh1236I	CGCG	5 cut(s)	99, 493, 495, 869, 1359
Bsh1285I	CGRYCG	1 cut(s)	51
BshFI	GGCC	2 cut(s)	347, 1413
BshNI	GGYRCC	5 cut(s)	137, 207, 352, 1288, 1376
BsiEI	CGRYCG	1 cut(s)	51
BsiHKAI	GWGCWC	1 cut(s)	1030
BsiSI	CCGG	3 cut(s)	47, 135, 211
BsiWI	CGTACG	1 cut(s)	1360
BslFI	GGGAC	3 cut(s)	600, 787, 830
BslI	CCNNNNNNNGG	7 cut(s)	33, 147, 496, 901, 1097, 1120, 1222
BsmAI	GTCTC	5 cut(s)	133, 168, 334, 950, 2004
BsmBI	CGTCTC	3 cut(s)	133, 950, 2004
BsmFI	GGGAC	3 cut(s)	600, 787, 830
BsnI	GGCC	2 cut(s)	347, 1413
Bso31I	GGTCTC	2 cut(s)	168, 334
Bsp1286I	GDGCHC	4 cut(s)	1030, 1114, 1159, 1920
Bsp1407I	TGTACA	1 cut(s)	1714
Bsp19I	CCATGG	2 cut(s)	348, 2213
BspACI	CCGC	7 cut(s)	90, 93, 97, 241, 491, 729, 1244
BspANI	GGCC	2 cut(s)	347, 1413
BspCNI	CTCAG	4 cut(s)	467, 756, 1279, 1736
BspFNI	CGCG	5 cut(s)	99, 493, 495, 869, 1359
BspLI	GGNNCC	9 cut(s)	109, 139, 209, 354, 775, 776, 1290, 1378, 2043
BspMI	ACCTGC	1 cut(s)	73
BspQI	GCTCTTC	1 cut(s)	1408
BspT107I	GGYRCC	5 cut(s)	137, 207, 352, 1288, 1376
BspTNI	GGTCTC	2 cut(s)	168, 334
BsrDI	GCAATG	1 cut(s)	1316
BsrFI	RCCGGY	1 cut(s)	46
BsrGI	TGTACA	1 cut(s)	1714
BsrI	ACTGG	4 cut(s)	414, 429, 802, 2159
BssAI	RCCGGY	1 cut(s)	46
BssECI	CCNNGG	5 cut(s)	133, 348, 578, 1968, 2213
BssNAI	GTATAC	1 cut(s)	562
BssNI	GRCGYC	2 cut(s)	138, 1289
BssSI	CACGAG	2 cut(s)	184, 1395
BssT1I	CCWWGG	3 cut(s)	348, 578, 2213
Bst1107I	GTATAC	1 cut(s)	562
Bst2BI	CACGAG	2 cut(s)	184, 1395
Bst2UI	CCWGG	3 cut(s)	1782, 1902, 1969
Bst4CI	ACNGT	7 cut(s)	155, 310, 536, 706, 794, 821, 2099
Bst6I	CTCTTC	2 cut(s)	256, 1408
BstACI	GRCGYC	2 cut(s)	138, 1289
BstAUI	TGTACA	1 cut(s)	1714
BstC8I	GCNNGC	5 cut(s)	97, 217, 230, 843, 1737
BstDEI	CTNAG	5 cut(s)	454, 764, 1266, 1723, 2106
BstDSI	CCRYGG	2 cut(s)	348, 2213
BstF5I	GGATG	6 cut(s)	152, 448, 676, 1717, 2001, 2223
BstFNI	CGCG	5 cut(s)	99, 493, 495, 869, 1359
BstH2I	RGCGCY	2 cut(s)	141, 1292
BstHHI	GCGC	6 cut(s)	101, 140, 495, 1264, 1291, 1359
BstMAI	GTCTC	5 cut(s)	133, 168, 334, 950, 2004
BstMCI	CGRYCG	1 cut(s)	51
BstMWI	GCNNNNNNNGC	9 cut(s)	98, 105, 157, 216, 225, 238, 264, 726, 881
BstNI	CCWGG	3 cut(s)	1782, 1902, 1969
BstNSI	RCATGY	1 cut(s)	1739
BstSCI	CCNGG	5 cut(s)	133, 210, 1780, 1900, 1967
BstSFI	CTRYAG	3 cut(s)	13, 1960, 2250
BstSLI	GKGCMC	1 cut(s)	1159
BstUI	CGCG	5 cut(s)	99, 493, 495, 869, 1359
BstV1I	GCAGC	5 cut(s)	561, 871, 893, 1479, 1822
BstV2I	GAAGAC	1 cut(s)	972
BstX2I	RGATCY	8 cut(s)	283, 663, 731, 965, 1005, 1081, 2041, 2155
BstXI	CCANNNNNNTGG	2 cut(s)	1285, 2069
BstYI	RGATCY	8 cut(s)	283, 663, 731, 965, 1005, 1081, 2041, 2155
BstZ17I	GTATAC	1 cut(s)	562
BsuI	GTATCC	1 cut(s)	1285
BsuRI	GGCC	2 cut(s)	347, 1413
BtgI	CCRYGG	2 cut(s)	348, 2213
BtsCI	GGATG	6 cut(s)	152, 448, 676, 1717, 2001, 2223
BtsI	GCAGTG	2 cut(s)	1062, 1832
BtsIMutI	CAGTG	5 cut(s)	151, 421, 436, 1062, 1832
BveI	ACCTGC	1 cut(s)	73
Cac8I	GCNNGC	5 cut(s)	97, 217, 230, 843, 1737
CaiI	CAGNNNCTG	1 cut(s)	1061
CfoI	GCGC	6 cut(s)	101, 140, 495, 1264, 1291, 1359
Cfr10I	RCCGGY	1 cut(s)	46
Cfr13I	GGNCC	1 cut(s)	774
CseI	GACGC	3 cut(s)	58, 875, 967
CsiI	ACCWGGT	1 cut(s)	1780
Csp6I	GTAC	6 cut(s)	645, 795, 1064, 1361, 1715, 1941
CviQI	GTAC	6 cut(s)	645, 795, 1064, 1361, 1715, 1941
DdeI	CTNAG	5 cut(s)	454, 764, 1266, 1723, 2106
DinI	GGCGCC	2 cut(s)	139, 1290
DraIII	CACNNNGTG	2 cut(s)	359, 1784
DrdI	GACNNNNNNGTC	2 cut(s)	83, 110
DriI	GACNNNNNGTC	1 cut(s)	2097
DseDI	GACNNNNNNGTC	2 cut(s)	83, 110
EaeI	YGGCCR	1 cut(s)	345
Eam1104I	CTCTTC	2 cut(s)	256, 1408
Eam1105I	GACNNNNNGTC	1 cut(s)	2097
EarI	CTCTTC	2 cut(s)	256, 1408
Ecl136II	GAGCTC	1 cut(s)	1028
Eco130I	CCWWGG	3 cut(s)	348, 578, 2213
Eco147I	AGGCCT	1 cut(s)	1413
Eco24I	GRGCYC	3 cut(s)	1030, 1114, 1920
Eco31I	GGTCTC	2 cut(s)	168, 334
Eco32I	GATATC	2 cut(s)	1252, 1351
Eco47I	GGWCC	1 cut(s)	774
Eco53kI	GAGCTC	1 cut(s)	1028
Eco57I	CTGAAG	1 cut(s)	1389
EcoICRI	GAGCTC	1 cut(s)	1028
EcoO109I	RGGNCCY	1 cut(s)	774
EcoRII	CCWGG	3 cut(s)	1780, 1900, 1967
EcoRV	GATATC	2 cut(s)	1252, 1351
EcoT14I	CCWWGG	3 cut(s)	348, 578, 2213
EcoT38I	GRGCYC	3 cut(s)	1030, 1114, 1920
EgeI	GGCGCC	2 cut(s)	139, 1290
EheI	GGCGCC	2 cut(s)	139, 1290
ErhI	CCWWGG	3 cut(s)	348, 578, 2213
Esp3I	CGTCTC	3 cut(s)	133, 950, 2004
FaqI	GGGAC	3 cut(s)	600, 787, 830
FauI	CCCGC	2 cut(s)	104, 498
FbaI	TGATCA	2 cut(s)	463, 1765
FblI	GTMKAC	3 cut(s)	72, 561, 2253
Fnu4HI	GCNGC	7 cut(s)	90, 93, 575, 882, 885, 1468, 1836
FokI	GGATG	6 cut(s)	159, 455, 683, 1724, 2008, 2230
FriOI	GRGCYC	3 cut(s)	1030, 1114, 1920
Fsp4HI	GCNGC	7 cut(s)	90, 93, 575, 882, 885, 1468, 1836
FspBI	CTAG	9 cut(s)	59, 255, 579, 675, 951, 969, 1415, 1730, 1938
GlaI	GCGC	6 cut(s)	100, 139, 494, 1263, 1290, 1358
GluI	GCNGC	7 cut(s)	90, 93, 575, 882, 885, 1468, 1836
GsaI	CCCAGC	2 cut(s)	894, 965
GsuI	CTGGAG	1 cut(s)	1895
HaeII	RGCGCY	2 cut(s)	141, 1292
HaeIII	GGCC	2 cut(s)	347, 1413
HapII	CCGG	3 cut(s)	47, 135, 211
HgaI	GACGC	3 cut(s)	58, 875, 967
HhaI	GCGC	6 cut(s)	101, 140, 495, 1264, 1291, 1359
Hin1I	GRCGYC	2 cut(s)	138, 1289
Hin6I	GCGC	6 cut(s)	99, 138, 493, 1262, 1289, 1357
HinP1I	GCGC	6 cut(s)	99, 138, 493, 1262, 1289, 1357
HincII	GTYRAC	1 cut(s)	73
HindII	GTYRAC	1 cut(s)	73
HpaII	CCGG	3 cut(s)	47, 135, 211
HphI	GGTGA	5 cut(s)	413, 524, 875, 1202, 1796
Hpy166II	GTNNAC	9 cut(s)	73, 487, 562, 1230, 1717, 1809, 2091, 2114, 2254
Hpy8I	GTNNAC	9 cut(s)	73, 487, 562, 1230, 1717, 1809, 2091, 2114, 2254
HpyAV	CCTTC	1 cut(s)	1941
HpyCH4III	ACNGT	7 cut(s)	155, 310, 536, 706, 794, 821, 2099
HpyCH4IV	ACGT	1 cut(s)	1011
HpyF10VI	GCNNNNNNNGC	9 cut(s)	98, 105, 157, 216, 225, 238, 264, 726, 881
HpyF3I	CTNAG	5 cut(s)	454, 764, 1266, 1723, 2106
HpySE526I	ACGT	1 cut(s)	1011
Hsp92I	GRCGYC	2 cut(s)	138, 1289
HspAI	GCGC	6 cut(s)	99, 138, 493, 1262, 1289, 1357
KasI	GGCGCC	2 cut(s)	137, 1288
KflI	GGGWCCC	1 cut(s)	774
Ksp22I	TGATCA	2 cut(s)	463, 1765
LguI	GCTCTTC	1 cut(s)	1408
LmnI	GCTCC	3 cut(s)	29, 113, 892
Lsp1109I	GCAGC	5 cut(s)	561, 871, 893, 1479, 1822
LweI	GCATC	4 cut(s)	535, 610, 661, 1986
MabI	ACCWGGT	1 cut(s)	1780
MaeI	CTAG	9 cut(s)	59, 255, 579, 675, 951, 969, 1415, 1730, 1938
MaeII	ACGT	1 cut(s)	1011
MaeIII	GTNAC	4 cut(s)	431, 753, 863, 2021
MfeI	CAATTG	1 cut(s)	1283
MflI	RGATCY	8 cut(s)	283, 663, 731, 965, 1005, 1081, 2041, 2155
MhlI	GDGCHC	4 cut(s)	1030, 1114, 1159, 1920
MlsI	TGGCCA	1 cut(s)	347
MluNI	TGGCCA	1 cut(s)	347
Mly113I	GGCGCC	2 cut(s)	138, 1289
MlyI	GAGTC	6 cut(s)	71, 446, 622, 705, 1591, 2234
MmeI	TCCRAC	4 cut(s)	124, 474, 688, 1326
Mox20I	TGGCCA	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	2142
MscI	TGGCCA	1 cut(s)	347
MslI	CAYNNNNRTG	3 cut(s)	861, 1035, 1164
Msp20I	TGGCCA	1 cut(s)	347
MspA1I	CMGCKG	2 cut(s)	160, 884
MspI	CCGG	3 cut(s)	47, 135, 211
MspR9I	CCNGG	5 cut(s)	135, 212, 1782, 1902, 1969
MunI	CAATTG	1 cut(s)	1283
MvaI	CCWGG	3 cut(s)	1782, 1902, 1969
MvnI	CGCG	5 cut(s)	99, 493, 495, 869, 1359
MwoI	GCNNNNNNNGC	9 cut(s)	98, 105, 157, 216, 225, 238, 264, 726, 881
NarI	GGCGCC	2 cut(s)	138, 1289
NciI	CCSGG	2 cut(s)	135, 212
NcoI	CCATGG	2 cut(s)	348, 2213
NlaIV	GGNNCC	9 cut(s)	109, 139, 209, 354, 775, 776, 1290, 1378, 2043
NmuCI	GTSAC	3 cut(s)	431, 863, 2021
NspI	RCATGY	1 cut(s)	1739
OliI	CACNNNNGTG	1 cut(s)	861
PaeI	GCATGC	1 cut(s)	1739
PceI	AGGCCT	1 cut(s)	1413
PciSI	GCTCTTC	1 cut(s)	1408
PcsI	WCGNNNNNNNCGW	2 cut(s)	111, 191
PdmI	GAANNNNTTC	1 cut(s)	2142
PfeI	GAWTC	5 cut(s)	166, 317, 446, 1661, 1887
Pfl23II	CGTACG	1 cut(s)	1360
PkrI	GCNGC	7 cut(s)	91, 94, 576, 883, 886, 1469, 1837
PleI	GAGTC	6 cut(s)	71, 446, 621, 705, 1591, 2234
PluTI	GGCGCC	2 cut(s)	141, 1292
PpsI	GAGTC	6 cut(s)	71, 446, 621, 705, 1591, 2234
PpuMI	RGGWCCY	1 cut(s)	774
PshBI	ATTAAT	1 cut(s)	540
Psp124BI	GAGCTC	1 cut(s)	1030
Psp5II	RGGWCCY	1 cut(s)	774
Psp6I	CCWGG	3 cut(s)	1780, 1900, 1967
PspFI	CCCAGC	2 cut(s)	890, 961
PspGI	CCWGG	3 cut(s)	1780, 1900, 1967
PspLI	CGTACG	1 cut(s)	1360
PspN4I	GGNNCC	9 cut(s)	109, 139, 209, 354, 775, 776, 1290, 1378, 2043
PspPI	GGNCC	1 cut(s)	774
PspPPI	RGGWCCY	1 cut(s)	774
PstNI	CAGNNNCTG	1 cut(s)	1061
PsuI	RGATCY	8 cut(s)	283, 663, 731, 965, 1005, 1081, 2041, 2155
PvuII	CAGCTG	2 cut(s)	160, 884
RsaI	GTAC	6 cut(s)	646, 796, 1065, 1362, 1716, 1942
RsaNI	GTAC	6 cut(s)	645, 795, 1064, 1361, 1715, 1941
RseI	CAYNNNNRTG	3 cut(s)	861, 1035, 1164
SacI	GAGCTC	1 cut(s)	1030
SalI	GTCGAC	1 cut(s)	71
SapI	GCTCTTC	1 cut(s)	1408
SatI	GCNGC	7 cut(s)	90, 93, 575, 882, 885, 1468, 1836
Sau96I	GGNCC	1 cut(s)	774
ScaI	AGTACT	2 cut(s)	646, 796
SchI	GAGTC	6 cut(s)	71, 446, 622, 705, 1591, 2234
ScrFI	CCNGG	5 cut(s)	135, 212, 1782, 1902, 1969
SduI	GDGCHC	4 cut(s)	1030, 1114, 1159, 1920
SexAI	ACCWGGT	1 cut(s)	1780
SfaNI	GCATC	4 cut(s)	535, 610, 661, 1986
SfcI	CTRYAG	3 cut(s)	13, 1960, 2250
SfoI	GGCGCC	2 cut(s)	139, 1290
SgrDI	CGTCGACG	1 cut(s)	71
SinI	GGWCC	1 cut(s)	774
SmiMI	CAYNNNNRTG	3 cut(s)	861, 1035, 1164
SphI	GCATGC	1 cut(s)	1739
SseBI	AGGCCT	1 cut(s)	1413
SsiI	CCGC	7 cut(s)	90, 93, 97, 241, 491, 729, 1244
SspDI	GGCGCC	2 cut(s)	137, 1288
SspMI	CTAG	9 cut(s)	59, 255, 579, 675, 951, 969, 1415, 1730, 1938
SstI	GAGCTC	1 cut(s)	1030
StuI	AGGCCT	1 cut(s)	1413
StyD4I	CCNGG	5 cut(s)	133, 210, 1780, 1900, 1967
StyI	CCWWGG	3 cut(s)	348, 578, 2213
TaaI	ACNGT	7 cut(s)	155, 310, 536, 706, 794, 821, 2099
TaiI	ACGT	1 cut(s)	1014
TatI	WGTACW	4 cut(s)	644, 794, 1714, 1940
TauI	GCSGC	2 cut(s)	92, 95
TfiI	GAWTC	5 cut(s)	166, 317, 446, 1661, 1887
TscAI	CASTG	5 cut(s)	158, 421, 436, 1062, 1832
TseFI	GTSAC	3 cut(s)	431, 863, 2021
TseI	GCWGC	5 cut(s)	574, 881, 884, 1467, 1835
Tsp45I	GTSAC	3 cut(s)	431, 863, 2021
TspDTI	ATGAA	4 cut(s)	158, 840, 1010, 2031
TspGWI	ACGGA	3 cut(s)	16, 100, 2182
TspRI	CASTG	5 cut(s)	158, 421, 436, 1062, 1832
VpaK11BI	GGWCC	1 cut(s)	774
VspI	ATTAAT	1 cut(s)	540
XapI	RAATTY	6 cut(s)	528, 1895, 2056, 2131, 2138, 2233
XceI	RCATGY	1 cut(s)	1739
XmaJI	CCTAGG	1 cut(s)	578
XmiI	GTMKAC	3 cut(s)	72, 561, 2253
XmnI	GAANNNNTTC	1 cut(s)	2142
XspI	CTAG	9 cut(s)	59, 255, 579, 675, 951, 969, 1415, 1730, 1938
ZrmI	AGTACT	2 cut(s)	646, 796

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.