MD08G1084400.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010161
Physical Location & Seq
Forward (+)
6985143 .. 6987168
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1084400.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGCGATGGAAAATCCCCCGAATTTCAAATACATGGGGAGGAACTTCAGTGATATGAGTAACGGCGACAACTCTTCTGCTTTCAGCGAGTGTAACAGCGACCGATCCGAAGAGTTTCCGACGACGTCGTCTCAGGGCCGGCGGCTTCTGATTGCCTGCGCTTCCGACAACTCCGACGATCTGATTCAACAGCTCGTGGCCGTCCTCGAAGACGGTTCGATTGAGGAGCAGAAGCAGGCGGCGATGGAGATCAGGCTCCTCGCCAAGAACAAGTCCGAAAATCGGCTCAAAATCGCCCGAAACGGCGCGATTAAGCCGTTGATTTCTCTCCTATCGTCGACCGATCTCCAGCTCCAGGAGTACGGTGTCACCGCGATACTCAACCTTTCGCTGTGCGACGAGAACAAGGACCTCATCGCTTCATCGGGAGCGATCAAGCCGCTTGTCCGGTCGCTCAAGACGGGGACCGCCACGGCGAAAGAGAACGCCGCCTGCGCTCTGCTCCGCCTCTCGCAAATCGAAGAGAACAAAGTCGCAATTGGACGGTCGGGTGCGATTCCGCTGCTGGTGAACCTTCTGGAGTACGGTGGTTTTCGCGGGAAGAAGGACGCGTCGACGGCTCTGTACTCGCTCTGCTCAGTCAAAGAGAACAAGATTAGAGCCGTACAATCGGGAATCATGAAGCCGCTGGTGGAATTGATGGCGGACTTCGGTTCGAACATGGTGGACAAGTCGGCGTACGTGCTGAGCGTCCTCGTATCGGTATCGGAGGCCCGCGCGGCGTTGGTGGAGGAAGGCGGGATTCCGGTGCTGGTGGAGATCATAGAGGTGGGGTCGCAGCGGCAGAAGGAGATATCGGTTGCGATATTGTTGCAGATTTGCGAGAACAGTGGAGTCCACCGTAATATGGTGGCCCGCGAAGGAGCGATTCCTCCCCTCGTCGCTTTGTCTCAGTCCGGCACCAATCGCGCCAAGCAAAAGGCGGAGACATTGACAGAGCTTCTCCGGCAACCGAGATCCGGCAAAGTCGCTGCACGAGCGTCCGACGTGTCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

353

Amino Acids

37.85

Weight (kDa)

6.67

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 56 - 192 5.2e-06 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 58 - 237 4.5e-13 ARMC5
ARM_ARMC5 PF24768 126 - 321 4.3e-14 ARMC5
Arm PF00514 133 - 171 9.7e-06 Armadillo/beta-catenin-like repeat
ARM_PUB PF25598 206 - 336 2.7e-12 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AatII GACGTC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 959
AccI GTMKAC 2 cut(s) 338, 614
AccII CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
AclWI GGATC 2 cut(s) 99, 1011
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 31
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 5 cut(s) 362, 584, 626, 666, 740
AfiI CCNNNNNNNGG 5 cut(s) 282, 355, 760, 907, 1019
AflIII ACRYGT 2 cut(s) 609, 1047
AgsI TTSAA 2 cut(s) 28, 188
AjiI CACGTC 1 cut(s) 1048
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 3 cut(s) 193, 352, 1000
AluI AGCT 3 cut(s) 193, 352, 1000
Alw26I GTCTC 3 cut(s) 135, 954, 980
AlwI GGATC 2 cut(s) 99, 1011
AoxI GGCC 4 cut(s) 136, 198, 771, 912
ApeKI GCWGC 3 cut(s) 562, 838, 1031
ApoI RAATTY 1 cut(s) 22
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 5 cut(s) 161, 308, 497, 779, 971
AspS9I GGNCC 5 cut(s) 136, 409, 465, 772, 913
AsuHPI GGTGA 2 cut(s) 361, 580
AsuII TTCGAA 1 cut(s) 716
AvaII GGWCC 2 cut(s) 409, 465
BanI GGYRCC 1 cut(s) 959
BauI CACGAG 2 cut(s) 194, 1035
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 549, 850, 1018
BccI CCATC 2 cut(s) 238, 694
BceAI ACGGC 7 cut(s) 79, 185, 301, 319, 489, 633, 647
BcgI CGANNNNNNTGC 4 cut(s) 544, 578, 853, 887
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 135, 954, 980
BglI GCCNNNNNGGC 1 cut(s) 779
BlpI GCTNAGC 1 cut(s) 746
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 409, 465
BmgBI CACGTC 1 cut(s) 1048
BmgT120I GGNCC 5 cut(s) 136, 409, 465, 772, 913
BmiI GGNNCC 3 cut(s) 257, 466, 961
BmrFI CCNGG 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 3 cut(s) 332, 338, 599
Bpu1102I GCTNAGC 1 cut(s) 746
Bpu14I TTCGAA 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 471
BsaWI WCCGGW 2 cut(s) 447, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 355, 760, 907, 1019
Bse118I RCCGGY 1 cut(s) 138
Bse8I GATNNNNATC 1 cut(s) 248
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 471
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 5 cut(s) 282, 355, 760, 907, 1019
BseMII CTCAG 4 cut(s) 146, 651, 737, 965
BseRI GAGGAG 2 cut(s) 239, 248
BseXI GCAGC 3 cut(s) 549, 850, 1018
BsgI GTGCAG 1 cut(s) 1017
Bsh1236I CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
Bsh1285I CGRYCG 4 cut(s) 103, 342, 452, 548
BshFI GGCC 4 cut(s) 138, 200, 773, 914
BshNI GGYRCC 1 cut(s) 959
BsiEI CGRYCG 4 cut(s) 103, 342, 452, 548
BsiSI CCGG 6 cut(s) 139, 448, 806, 957, 1006, 1020
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 478
BslI CCNNNNNNNGG 5 cut(s) 282, 355, 760, 907, 1019
BsmAI GTCTC 3 cut(s) 135, 954, 980
BsmBI CGTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 478
BsnI GGCC 4 cut(s) 138, 200, 773, 914
Bsp119I TTCGAA 1 cut(s) 716
Bsp143I GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
Bsp1720I GCTNAGC 1 cut(s) 746
BspANI GGCC 4 cut(s) 138, 200, 773, 914
BspCNI CTCAG 4 cut(s) 145, 650, 738, 964
BspFNI CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 3 cut(s) 257, 466, 961
BspPI GGATC 2 cut(s) 99, 1011
BspT104I TTCGAA 1 cut(s) 716
BspT107I GGYRCC 1 cut(s) 959
BsrFI RCCGGY 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 2 cut(s) 194, 1035
Bst2BI CACGAG 2 cut(s) 194, 1035
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 6 cut(s) 215, 365, 546, 587, 890, 902
Bst6I CTCTTC 3 cut(s) 79, 105, 516
BstACI GRCGYC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 742
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 6 cut(s) 140, 157, 237, 493, 775, 916
BstDEI CTNAG 4 cut(s) 132, 637, 746, 951
BstDSI CCRYGG 1 cut(s) 471
BstFNI CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
BstHHI GCGC 5 cut(s) 161, 308, 497, 779, 971
BstKTI GATC 7 cut(s) 107, 181, 252, 346, 435, 822, 1019
BstMAI GTCTC 3 cut(s) 135, 954, 980
BstMBI GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
BstMCI CGRYCG 4 cut(s) 103, 342, 452, 548
BstMWI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstUI CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
BstV1I GCAGC 3 cut(s) 549, 850, 1018
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 1016
BstYI RGATCY 1 cut(s) 1016
BsuRI GGCC 4 cut(s) 138, 200, 773, 914
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 3 cut(s) 20, 257, 400
BtrI CACGTC 1 cut(s) 1048
BtsIMutI CAGTG 2 cut(s) 55, 895
Cac8I GCNNGC 6 cut(s) 140, 157, 237, 493, 775, 916
CciI TCATGA 1 cut(s) 678
CfoI GCGC 5 cut(s) 161, 308, 497, 779, 971
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 5 cut(s) 136, 409, 465, 772, 913
CseI GACGC 4 cut(s) 600, 617, 739, 1029
Csp6I GTAC 5 cut(s) 361, 583, 625, 665, 739
CviAII CATG 3 cut(s) 34, 679, 721
CviQI GTAC 5 cut(s) 361, 583, 625, 665, 739
DdeI CTNAG 4 cut(s) 132, 637, 746, 951
DpnI GATC 7 cut(s) 106, 180, 251, 345, 434, 821, 1018
DpnII GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 3 cut(s) 79, 105, 516
EarI CTCTTC 3 cut(s) 79, 105, 516
EciI GGCGGA 3 cut(s) 494, 719, 998
Eco32I GATATC 1 cut(s) 855
Eco47I GGWCC 2 cut(s) 409, 465
Eco57I CTGAAG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 855
Esp3I CGTCTC 1 cut(s) 135
FaeI CATG 3 cut(s) 37, 682, 724
FaiI YATR 7 cut(s) 35, 56, 680, 722, 824, 908, 1057
FaqI GGGAC 1 cut(s) 478
FatI CATG 3 cut(s) 33, 678, 720
FauI CCCGC 4 cut(s) 590, 782, 791, 923
FblI GTMKAC 2 cut(s) 338, 614
GlaI GCGC 5 cut(s) 160, 307, 496, 778, 970
GsuI CTGGAG 3 cut(s) 332, 338, 599
HaeIII GGCC 4 cut(s) 138, 200, 773, 914
HapII CCGG 6 cut(s) 139, 448, 806, 957, 1006, 1020
HgaI GACGC 4 cut(s) 600, 617, 739, 1029
HhaI GCGC 5 cut(s) 161, 308, 497, 779, 971
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 37, 682, 724
Hin6I GCGC 5 cut(s) 159, 306, 495, 777, 969
HinP1I GCGC 5 cut(s) 159, 306, 495, 777, 969
HincII GTYRAC 2 cut(s) 339, 615
HindII GTYRAC 2 cut(s) 339, 615
HinfI GANTC 6 cut(s) 184, 556, 675, 802, 894, 928
HpaII CCGG 6 cut(s) 139, 448, 806, 957, 1006, 1020
HphI GGTGA 2 cut(s) 361, 580
Hpy166II GTNNAC 5 cut(s) 339, 571, 615, 727, 898
Hpy188I TCNGA 9 cut(s) 109, 120, 150, 166, 175, 183, 277, 769, 1045
Hpy188III TCNNGA 5 cut(s) 426, 457, 578, 672, 679
Hpy8I GTNNAC 5 cut(s) 339, 571, 615, 727, 898
HpyAV CCTTC 5 cut(s) 584, 598, 788, 841, 914
HpyCH4III ACNGT 6 cut(s) 215, 365, 546, 587, 890, 902
HpyCH4IV ACGT 3 cut(s) 125, 741, 1047
HpyCH4V TGCA 2 cut(s) 874, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
HpyF3I CTNAG 4 cut(s) 132, 637, 746, 951
HpySE526I ACGT 3 cut(s) 125, 741, 1047
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 37, 682, 724
HspAI GCGC 5 cut(s) 159, 306, 495, 777, 969
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Kzo9I GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
LmnI GCTCC 6 cut(s) 226, 261, 357, 428, 507, 923
Lsp1109I GCAGC 3 cut(s) 549, 850, 1018
MaeII ACGT 3 cut(s) 125, 741, 1047
MaeIII GTNAC 3 cut(s) 59, 92, 367
MalI GATC 7 cut(s) 106, 180, 251, 345, 434, 821, 1018
MboI GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
MboII GAAGA 5 cut(s) 66, 122, 221, 533, 613
MfeI CAATTG 1 cut(s) 537
MflI RGATCY 1 cut(s) 1016
MluCI AATT 3 cut(s) 22, 537, 695
MluI ACGCGT 1 cut(s) 609
MlyI GAGTC 1 cut(s) 903
MmeI TCCRAC 3 cut(s) 143, 189, 198
MroNI GCCGGC 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 1 cut(s) 827
MspA1I CMGCKG 3 cut(s) 562, 688, 841
MspI CCGG 6 cut(s) 139, 448, 806, 957, 1006, 1020
MspR9I CCNGG 1 cut(s) 356
MunI CAATTG 1 cut(s) 537
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 8 cut(s) 308, 374, 597, 611, 777, 779, 918, 969
MwoI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
NaeI GCCGGC 1 cut(s) 140
NdeII GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 3 cut(s) 37, 682, 724
NlaIV GGNNCC 3 cut(s) 257, 466, 961
NmuCI GTSAC 1 cut(s) 367
NspV TTCGAA 1 cut(s) 716
PagI TCATGA 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 747
PdiI GCCGGC 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 5 cut(s) 184, 556, 675, 802, 928
Pfl23II CGTACG 1 cut(s) 738
PflFI GACNNNGTC 2 cut(s) 124, 127
PfoI TCCNGGA 1 cut(s) 354
PleI GAGTC 1 cut(s) 902
PpsI GAGTC 1 cut(s) 902
Ppu21I YACGTR 1 cut(s) 742
PpuMI RGGWCCY 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 3 cut(s) 257, 466, 961
PspPI GGNCC 5 cut(s) 136, 409, 465, 772, 913
PspPPI RGGWCCY 1 cut(s) 409
PsuI RGATCY 1 cut(s) 1016
PsyI GACNNNGTC 2 cut(s) 124, 127
RsaI GTAC 5 cut(s) 362, 584, 626, 666, 740
RsaNI GTAC 5 cut(s) 361, 583, 625, 665, 739
RseI CAYNNNNRTG 1 cut(s) 827
SalI GTCGAC 2 cut(s) 337, 613
SaqAI TTAA 1 cut(s) 312
Sau3AI GATC 7 cut(s) 104, 178, 249, 343, 432, 819, 1016
Sau96I GGNCC 5 cut(s) 136, 409, 465, 772, 913
SchI GAGTC 1 cut(s) 903
ScrFI CCNGG 1 cut(s) 356
SfuI TTCGAA 1 cut(s) 716
SgrDI CGTCGACG 1 cut(s) 613
SinI GGWCC 2 cut(s) 409, 465
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 22, 537, 695
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 6 cut(s) 215, 365, 546, 587, 890, 902
TaiI ACGT 3 cut(s) 128, 744, 1050
TaqI TCGA 6 cut(s) 207, 218, 338, 519, 614, 716
TaqII GACCGA 2 cut(s) 117, 356
TasI AATT 3 cut(s) 22, 537, 695
TatI WGTACW 1 cut(s) 624
TauI GCSGC 7 cut(s) 145, 242, 442, 491, 688, 782, 844
TfiI GAWTC 5 cut(s) 184, 556, 675, 802, 928
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 55, 895
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 562, 838, 1031
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 2 cut(s) 411, 695
TspRI CASTG 2 cut(s) 55, 895
Tth111I GACNNNGTC 2 cut(s) 124, 127
VpaK11BI GGWCC 2 cut(s) 409, 465
XapI RAATTY 1 cut(s) 22
XmiI GTMKAC 2 cut(s) 338, 614
XmnI GAANNNNTTC 1 cut(s) 114
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.