MD15G1070600.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010161
Physical Location & Seq
Forward (+)
4808322 .. 4810476
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1070600.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGAGATGGAAAATCCAGCGAATTTCAGTTACATGGGGAGGAACTTCAGTGATCTGAGCAACGGGGATAACTCCTCTGCTTTCAGCGAGTGTAACAGCGATAGATCCGAAGAGTTCCCGACGACTTCGTCCCAGAGCCGGCGGCTTCTGATTGCCTGCGCTTCCGACAACTCCGACGATATGATTCAACAGCTCGTCGCCGTGCTCGAGGCCGGTTCGACTGAGGCGCAGAAGCAGGCGGCGATGGAAATCAGGCTCCTCGCTAAGAACAAGTCCGAAAATCGGCTCAAAATCGCCCGAAACGGCGCGATTAAGCCATTGATTTCGCTCCTATCGTCGTCTGATCTCCAGCTCCAGGAGTACGGCGTCACCGCGATTCTCAACCTCTCGCTGTGCGACGAGAATAAGGAGCTCATCGCTTCGTCGGGAGCAATCAAGCCGCTGGTTCGGTCGCTCAAGACGGGGACCGCCACAGCGAAAGAGAACGCCGCCTGCGCTCTGCTCCGCCTCTCGCAAATCGAAGAGAACAAAGCCGCAATCGGACGGTCGGGAGCGATTCCGCTTCTGGTGAACCTTCTGGAGTGTGGAGGCTTTCGTGGGAAGAAGGACGCGTCGACGGCTTTGTACTCGCTGTGCTCGGTCAAAGAGAACAAGATTAGAGCCGTCGAGTCGGGAATCATGAAGCCGCTGGTGGAATTGATGGCGGACTTCGGGTCGAACATGGTGGACAAGTCGGCGTACGTGCTGAGCGTCCTCGTGTCGGTGCCGGAGGCCCGCGCAGCATTGGTGGAGGAAGGTGGGATTCCGGTGCTGGTGGAGATTGTAGAGGTGGGGTCGCAGCGGCAGAAGGAGATATCGGTGGCGATATTGCTGAAGCTTTGCGAGAACAGTGGGGTCCACCGTAACATGGTCGTCCGCGAAGGAGCGATTCCTCCCCTCGTCGCTTTGTCTCAGTCCGGCACCAATCGCGCCAAGCAAAAGGCGGAGACATTGACAGAGCTTCTACGGCAACCGAGGTCCGGCAACGTCGCTGCACCAGCGTCAGACGTGTCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

353

Amino Acids

37.62

Weight (kDa)

5.8

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 56 - 192 5.7e-06 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 58 - 237 1e-13 ARMC5
Arm PF00514 175 - 213 2.4e-06 Armadillo/beta-catenin-like repeat
ARM_PUB PF25598 207 - 337 3.4e-13 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 207 - 328 6.8e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1057
AasI GACNNNNNNGTC 1 cut(s) 127
AccB1I GGYRCC 2 cut(s) 763, 959
AccI GTMKAC 1 cut(s) 614
AccII CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 22
AcuI CTGAAG 2 cut(s) 31, 893
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 3 cut(s) 362, 626, 740
AfiI CCNNNNNNNGG 6 cut(s) 138, 282, 355, 760, 907, 1019
AflIII ACRYGT 2 cut(s) 609, 1047
AgsI TTSAA 1 cut(s) 188
AhdI GACNNNNNGTC 1 cut(s) 712
AjiI CACGTC 1 cut(s) 1048
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 5 cut(s) 193, 352, 412, 877, 1000
AluI AGCT 5 cut(s) 193, 352, 412, 877, 1000
Alw21I GWGCWC 3 cut(s) 207, 414, 638
Alw26I GTCTC 2 cut(s) 954, 980
AlwI GGATC 1 cut(s) 99
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 2 cut(s) 210, 771
ApeKI GCWGC 3 cut(s) 779, 838, 1031
ApoI RAATTY 1 cut(s) 22
AspLEI GCGC 6 cut(s) 161, 229, 308, 497, 779, 971
AspS9I GGNCC 4 cut(s) 465, 772, 895, 1017
AsuHPI GGTGA 2 cut(s) 361, 580
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 3 cut(s) 465, 895, 1017
BanI GGYRCC 2 cut(s) 763, 959
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 1 cut(s) 755
Bbv12I GWGCWC 3 cut(s) 207, 414, 638
BbvI GCAGC 3 cut(s) 791, 850, 1018
BccI CCATC 2 cut(s) 238, 694
BceAI ACGGC 6 cut(s) 185, 319, 379, 633, 647, 1022
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 954, 980
BlpI GCTNAGC 1 cut(s) 746
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 3 cut(s) 465, 895, 1017
BmeRI GACNNNNNGTC 1 cut(s) 712
BmeT110I CYCGRG 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 1048
BmgT120I GGNCC 4 cut(s) 465, 772, 895, 1017
BmiI GGNNCC 5 cut(s) 257, 466, 765, 896, 961
BmrFI CCNGG 1 cut(s) 356
BpmI CTGGAG 3 cut(s) 332, 338, 599
Bpu1102I GCTNAGC 1 cut(s) 746
BpuEI CTTGAG 1 cut(s) 440
BsaAI YACGTR 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 1013
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 6 cut(s) 138, 282, 355, 760, 907, 1019
Bse118I RCCGGY 2 cut(s) 138, 212
Bse8I GATNNNNATC 1 cut(s) 248
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 1013
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 6 cut(s) 138, 282, 355, 760, 907, 1019
BseMII CTCAG 4 cut(s) 47, 213, 737, 965
BseRI GAGGAG 2 cut(s) 64, 248
BseXI GCAGC 3 cut(s) 791, 850, 1018
BsgI GTGCAG 1 cut(s) 1017
Bsh1236I CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
Bsh1285I CGRYCG 2 cut(s) 452, 548
BshFI GGCC 2 cut(s) 212, 773
BshNI GGYRCC 2 cut(s) 763, 959
BsiEI CGRYCG 2 cut(s) 452, 548
BsiHKAI GWGCWC 3 cut(s) 207, 414, 638
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 6 cut(s) 139, 213, 767, 806, 957, 1020
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 2 cut(s) 115, 478
BslI CCNNNNNNNGG 6 cut(s) 138, 282, 355, 760, 907, 1019
BsmAI GTCTC 2 cut(s) 954, 980
BsmFI GGGAC 2 cut(s) 115, 478
BsnI GGCC 2 cut(s) 212, 773
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 3 cut(s) 207, 414, 638
Bsp143I GATC 3 cut(s) 52, 104, 343
Bsp1720I GCTNAGC 1 cut(s) 746
BspANI GGCC 2 cut(s) 212, 773
BspCNI CTCAG 4 cut(s) 48, 214, 738, 964
BspFNI CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 5 cut(s) 257, 466, 765, 896, 961
BspPI GGATC 1 cut(s) 99
BspT107I GGYRCC 2 cut(s) 763, 959
BsrFI RCCGGY 2 cut(s) 138, 212
BssAI RCCGGY 2 cut(s) 138, 212
BssECI CCNNGG 1 cut(s) 1013
BssMI GATC 3 cut(s) 52, 104, 343
BssNI GRCGYC 1 cut(s) 366
BssSI CACGAG 1 cut(s) 755
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 3 cut(s) 546, 890, 902
Bst6I CTCTTC 2 cut(s) 105, 516
BstACI GRCGYC 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 5 cut(s) 140, 157, 237, 493, 775
BstDEI CTNAG 5 cut(s) 56, 222, 264, 746, 951
BstFNI CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
BstHHI GCGC 6 cut(s) 161, 229, 308, 497, 779, 971
BstKTI GATC 3 cut(s) 55, 107, 346
BstMAI GTCTC 2 cut(s) 954, 980
BstMBI GATC 3 cut(s) 52, 104, 343
BstMCI CGRYCG 2 cut(s) 452, 548
BstMWI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstUI CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
BstV1I GCAGC 3 cut(s) 791, 850, 1018
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuRI GGCC 2 cut(s) 212, 773
BtgZI GCGATG 2 cut(s) 257, 400
BtrI CACGTC 1 cut(s) 1048
BtsIMutI CAGTG 2 cut(s) 55, 895
Cac8I GCNNGC 5 cut(s) 140, 157, 237, 493, 775
CciI TCATGA 1 cut(s) 678
CfoI GCGC 6 cut(s) 161, 229, 308, 497, 779, 971
Cfr10I RCCGGY 2 cut(s) 138, 212
Cfr13I GGNCC 4 cut(s) 465, 772, 895, 1017
CseI GACGC 5 cut(s) 355, 600, 617, 739, 1029
Csp6I GTAC 3 cut(s) 361, 625, 739
CviAII CATG 4 cut(s) 34, 679, 721, 907
CviQI GTAC 3 cut(s) 361, 625, 739
DdeI CTNAG 5 cut(s) 56, 222, 264, 746, 951
DpnI GATC 3 cut(s) 54, 106, 345
DpnII GATC 3 cut(s) 52, 104, 343
DrdI GACNNNNNNGTC 1 cut(s) 127
DriI GACNNNNNGTC 1 cut(s) 712
DseDI GACNNNNNNGTC 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 105, 516
Eam1105I GACNNNNNGTC 1 cut(s) 712
EarI CTCTTC 2 cut(s) 105, 516
EciI GGCGGA 3 cut(s) 494, 719, 998
Ecl136II GAGCTC 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 855
Eco47I GGWCC 3 cut(s) 465, 895, 1017
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 31, 893
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 855
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 4 cut(s) 37, 682, 724, 910
FaiI YATR 6 cut(s) 35, 182, 680, 722, 908, 1057
FaqI GGGAC 2 cut(s) 115, 478
FatI CATG 4 cut(s) 33, 678, 720, 906
FauI CCCGC 1 cut(s) 782
FblI GTMKAC 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 414
GlaI GCGC 6 cut(s) 160, 228, 307, 496, 778, 970
GsuI CTGGAG 3 cut(s) 332, 338, 599
HaeIII GGCC 2 cut(s) 212, 773
HapII CCGG 6 cut(s) 139, 213, 767, 806, 957, 1020
HgaI GACGC 5 cut(s) 355, 600, 617, 739, 1029
HhaI GCGC 6 cut(s) 161, 229, 308, 497, 779, 971
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 4 cut(s) 37, 682, 724, 910
Hin6I GCGC 6 cut(s) 159, 227, 306, 495, 777, 969
HinP1I GCGC 6 cut(s) 159, 227, 306, 495, 777, 969
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 7 cut(s) 184, 376, 556, 668, 675, 802, 928
HpaII CCGG 6 cut(s) 139, 213, 767, 806, 957, 1020
HphI GGTGA 2 cut(s) 361, 580
Hpy166II GTNNAC 4 cut(s) 571, 615, 727, 898
Hpy188I TCNGA 9 cut(s) 57, 109, 150, 166, 175, 277, 343, 542, 1045
Hpy188III TCNNGA 7 cut(s) 118, 426, 457, 549, 578, 672, 679
Hpy8I GTNNAC 4 cut(s) 571, 615, 727, 898
HpyAV CCTTC 5 cut(s) 584, 598, 788, 841, 914
HpyCH4III ACNGT 3 cut(s) 546, 890, 902
HpyCH4IV ACGT 3 cut(s) 741, 1026, 1047
HpyCH4V TGCA 1 cut(s) 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
HpyF3I CTNAG 5 cut(s) 56, 222, 264, 746, 951
HpySE526I ACGT 3 cut(s) 741, 1026, 1047
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 4 cut(s) 37, 682, 724, 910
HspAI GCGC 6 cut(s) 159, 227, 306, 495, 777, 969
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Kzo9I GATC 3 cut(s) 52, 104, 343
LmnI GCTCC 8 cut(s) 261, 333, 357, 409, 428, 507, 551, 923
Lsp1109I GCAGC 3 cut(s) 791, 850, 1018
MaeII ACGT 3 cut(s) 741, 1026, 1047
MaeIII GTNAC 4 cut(s) 29, 92, 367, 902
MalI GATC 3 cut(s) 54, 106, 345
MboI GATC 3 cut(s) 52, 104, 343
MboII GAAGA 3 cut(s) 122, 533, 613
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 3 cut(s) 207, 414, 638
MluCI AATT 2 cut(s) 22, 695
MluI ACGCGT 1 cut(s) 609
MlyI GAGTC 1 cut(s) 677
MmeI TCCRAC 2 cut(s) 189, 198
MroNI GCCGGC 1 cut(s) 138
MseI TTAA 1 cut(s) 312
MspA1I CMGCKG 3 cut(s) 442, 688, 841
MspI CCGG 6 cut(s) 139, 213, 767, 806, 957, 1020
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 6 cut(s) 308, 374, 611, 777, 918, 969
MwoI GCNNNNNNNGC 6 cut(s) 494, 617, 779, 966, 1006, 1037
NaeI GCCGGC 1 cut(s) 140
NdeII GATC 3 cut(s) 52, 104, 343
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 4 cut(s) 37, 682, 724, 910
NlaIV GGNNCC 5 cut(s) 257, 466, 765, 896, 961
NmuCI GTSAC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 678
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 332, 747
PdiI GCCGGC 1 cut(s) 140
PfeI GAWTC 6 cut(s) 184, 376, 556, 675, 802, 928
Pfl23II CGTACG 1 cut(s) 738
PflFI GACNNNGTC 1 cut(s) 127
PfoI TCCNGGA 1 cut(s) 354
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
Ppu21I YACGTR 1 cut(s) 742
PsiI TTATAA 1 cut(s) 1057
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 5 cut(s) 257, 466, 765, 896, 961
PspPI GGNCC 4 cut(s) 465, 772, 895, 1017
PspXI VCTCGAGB 1 cut(s) 206
PsuI RGATCY 1 cut(s) 104
PsyI GACNNNGTC 1 cut(s) 127
RsaI GTAC 3 cut(s) 362, 626, 740
RsaNI GTAC 3 cut(s) 361, 625, 739
SacI GAGCTC 1 cut(s) 414
SalI GTCGAC 1 cut(s) 613
SaqAI TTAA 1 cut(s) 312
Sau3AI GATC 3 cut(s) 52, 104, 343
Sau96I GGNCC 4 cut(s) 465, 772, 895, 1017
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 3 cut(s) 207, 414, 638
Sfr274I CTCGAG 1 cut(s) 206
SgrDI CGTCGACG 1 cut(s) 613
SinI GGWCC 3 cut(s) 465, 895, 1017
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 206, 455
SmoI CTYRAG 2 cut(s) 206, 455
Sse9I AATT 2 cut(s) 22, 695
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 546, 890, 902
TaiI ACGT 3 cut(s) 744, 1029, 1050
TaqI TCGA 6 cut(s) 207, 218, 519, 614, 666, 716
TaqII GACCGA 2 cut(s) 438, 628
TasI AATT 2 cut(s) 22, 695
TatI WGTACW 1 cut(s) 624
TauI GCSGC 7 cut(s) 145, 242, 442, 491, 536, 688, 844
TfiI GAWTC 6 cut(s) 184, 376, 556, 675, 802, 928
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 55, 895
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 779, 838, 1031
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 695
TspRI CASTG 2 cut(s) 55, 895
Tth111I GACNNNGTC 1 cut(s) 127
VpaK11BI GGWCC 3 cut(s) 465, 895, 1017
XapI RAATTY 1 cut(s) 22
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.