Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009660

Physical Location & Seq

Genomic Coordinates Forward (+)

10656603 .. 10662170

Gene Structure 5568 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1116500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGGAGCAGAGCGAAACGGTCTCCGAGGTGGCGCCGAAGAAATTGGCGAGGCAATTGGATTTCACGACGGTGTACCGAGCGTCGGCGAGCGCGGCGTTGACGGACGTACAGCTACAGCTACAGACACCGTCACAGGCGCAGCAGCAGCCACAAATCTCACCGGCAAGACCGCAGTTGCATTTGCATTTACAGTCGCCGCAGCCTGCGGCAGTTCAGTTGTCGAAGCAGTTAACGCCGCCGCCGGCGATTCCGCAGTTTCAGGCTCGGCGGAGTCCGGCGCCGCCCGCAAACGTTCGGATTCCGCACCCGGTGCATAAGCTTTCGCAAGTGAGAAAACAAGACTCTCCTGTATCACGCCAACGTGGCGATGAAAAAGATTGTACTCCAAAGAAGCAGAAGCAGTGCAATTGTAAAAACTCTCGGTGCCTGAAGTTGTACTGTGAGTGCTTTGCAGCTGGAATTTATTGTGATGGTTGCAACTGCTCAAATTGTCACAATAATGTGAACAATGAAGCTGCTAGGCAAGACGCAAGGGGAGCAATTTTAGAGCGTAATCCAAATGCTTTCCAACCAAAGATTGCTAGCAGCCCACAAGAATCTCGTGATGGTAGGGAAGATGTTAGGGAAATTCAAGCGGCTGGAAAGCACAACAAAGGATGCCATTGTAAGAAATCAGGCTGCCTCAAGAAGTATTGTGAGTGCTTCCAAGCCAATATTCTATGCTCTGAAAATTGTAAATGCATGGACTGCAAGAACTTTGAAGGAAGTGAAGAGAGAAGAGCTCTCTATCATGAAGGCCACAATACCGCGGCCTACATGCAACAGGCTAATGCGGCCATTAGTGGGGCCATTGGAACCTCTGGTTATGGGACCCCTCTGGTGTCCAGAAAAAGAAAAGGCTATGAACTTTATTTTACAACTGGAAATCAATCCACTCATCCAATTGCACAACCTCAACAGGAAAATCATCTAAGACCTCATATGGCCTCTTCTTCTCAGTTGTCTGTTCCTATTTTTCGTGCTGATGCAGCTGTACCAAGACCTTCAAAAACCACTATATACAGATCTCCATTGGCGGATATCATCCAACCAAAGAATGTAAAGGATCTGTGCTCACGTTTGGTTGTAGTTGCAGGAGCAGCTGCTAAGGCACTTGCAGGAGATATGCAAAGAGAGACAATCAATACTAGTACCACTTCATCCACTCAAGAGAGGGAAGAACATAAAATGGAACATGATAAGCAGCACGACAACCAATTAGGAGTGAACGAAGCAGATAGAGAAGTAAGTACCTTTTCTGGATCAAATGGAGTTGATGTGCAAAATAGTAGGCCAATGTCCCCTGTAACTCTTGCCCTGATGTGTGATGAACAAGATAAGATGTTTATGGAAGCTGGATTGCCAAAGGGTGCGGGAACTGATAATCCAAGTATGCCTGAGAAGCCAACTCAGGAAATAGGTTTCACAGAGATTTACGCGGAACAGGAAAGGCTTGTCCTGACCGGGTTCAGGGATTTCCTTAACCATCTTATTACTCGGGGGAGCATAAAAGAAACAATGTGTTCTCCCCAAGCCAAAACGGAGAGGTTAAGCCAGAAAGAATCCGTACAGGTTGGAACAGCAAAACCAAACACTGAACCCAGGTACCAGAAAGAGGTCTACATCAACGGCATTGTGAAATCTCCACTTTCATCAGCAAACGGTAAAATTCTTCAGTCGGCAACGACAGTCACTTCTGGTGACAATGATCTCACTCTAAAGTTGGATCTCGTTGAATGGCTGGGATTGAAAACCTGGGACTTGGTAGAGGAGCTAGAAAAAGCCTATCCCAGGAAAGCACTTTTCCAAAAAGCCATCCTATACTACAGTACGATTATTCGATTACCGTATTTGCGGAGCTTCCTTGCATCTTCCATAAACTGTATTTTCGTTCTGATTGTTTTGTTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

TCR

Protein Analysis

651

Amino Acids

72.02

Weight (kDa)

8.78

Isoelectric Point (pI)

55.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TCR	PF03638	132 - 167	2.3e-11	Tesmin/TSO1-like CXC domain, cysteine-rich domain
TCR	PF03638	218 - 252	2.6e-12	Tesmin/TSO1-like CXC domain, cysteine-rich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009660)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g35890 FvH4_2g35890 FvH4_2g35890 FvH4_2g35890
malus_domestica	MD08G1116500.v1.1 MD15G1096100.v1.1
prunus_persica	Prupe.1G451000_v2.0.a1 Prupe.1G451000_v2.0.a1
pyrus_communis	pycom08g09710 pycom15g08830
rosa_chinensis	RchiOBHm_Chr6g0305931
rosa_laevigata	RLG00000010845
rosa_multiflora	Rmu_sc0001809.1_g000050 Rmu_sc0039000.1_g000001
rosa_roxburghii	Rroxscaffold_7G00162210
rosa_rugosa	Rorug06G0345400
rosa_samantha	Rh6AG457000 Rh6AG457100 Rh6BG444300 Rh6CG470900 Rh6DG457600
rosa_wichuraiana	Rw6G039870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1644
AccB1I	GGYRCC	4 cut(s)	31, 277, 423, 1644
AccI	GTMKAC	1 cut(s)	1659
AccII	CGCG	3 cut(s)	92, 809, 1478
AclI	AACGTT	1 cut(s)	291
AclWI	GGATC	3 cut(s)	1113, 1309, 1773
AcoI	YGGCCR	1 cut(s)	834
AcsI	RAATTY	3 cut(s)	459, 627, 1707
AcuI	CTGAAG	2 cut(s)	449, 1697
AcyI	GRCGYC	2 cut(s)	32, 278
AdeI	CACNNNGTG	1 cut(s)	310
AfiI	CCNNNNNNNGG	5 cut(s)	82, 843, 1509, 1654, 1830
AgsI	TTSAA	5 cut(s)	632, 761, 1047, 1775, 1789
AhlI	ACTAGT	1 cut(s)	1187
AjnI	CCWGG	3 cut(s)	1640, 1793, 1829
AjuI	GAANNNNNNNTTGG	2 cut(s)	699, 731
AleI	CACNNNNGTG	1 cut(s)	68
AloI	GAACNNNNNNTCC	2 cut(s)	1408, 1440
Alw21I	GWGCWC	2 cut(s)	784, 1115
Alw26I	GTCTC	2 cut(s)	25, 1169
AlwI	GGATC	3 cut(s)	1113, 1309, 1773
Ama87I	CYCGRG	1 cut(s)	1536
AoxI	GGCC	6 cut(s)	796, 810, 834, 846, 984, 1331
ApoI	RAATTY	3 cut(s)	459, 627, 1707
Asp718I	GGTACC	1 cut(s)	1644
AspLEI	GCGC	4 cut(s)	34, 92, 139, 280
AspS9I	GGNCC	2 cut(s)	846, 870
AsuC2I	CCSGG	2 cut(s)	308, 1504
AsuHPI	GGTGA	2 cut(s)	150, 1751
AsuNHI	GCTAGC	1 cut(s)	581
AvaI	CYCGRG	1 cut(s)	1536
AvaII	GGWCC	1 cut(s)	870
BaeI	ACNNNNGTAYC	2 cut(s)	1628, 1661
BanI	GGYRCC	4 cut(s)	31, 277, 423, 1644
BanII	GRGCYC	1 cut(s)	784
BauI	CACGAG	1 cut(s)	600
Bbv12I	GWGCWC	2 cut(s)	784, 1115
BccI	CCATC	4 cut(s)	464, 599, 1533, 1862
BceAI	ACGGC	1 cut(s)	1684
BciT130I	CCWGG	3 cut(s)	1642, 1795, 1831
BcnI	CCSGG	2 cut(s)	308, 1504
BcoDI	GTCTC	2 cut(s)	25, 1169
BcuI	ACTAGT	1 cut(s)	1187
BfaI	CTAG	4 cut(s)	519, 582, 1188, 1814
BfmI	CTRYAG	3 cut(s)	113, 119, 1864
BfoI	RGCGCY	2 cut(s)	35, 281
BglI	GCCNNNNNGGC	1 cut(s)	363
BglII	AGATCT	1 cut(s)	1064
Bme1390I	CCNGG	5 cut(s)	308, 1504, 1642, 1795, 1831
Bme18I	GGWCC	1 cut(s)	870
BmeT110I	CYCGRG	1 cut(s)	1536
BmgT120I	GGNCC	2 cut(s)	846, 870
BmiI	GGNNCC	8 cut(s)	33, 279, 425, 847, 856, 871, 872, 1646
BmrFI	CCNGG	5 cut(s)	308, 1504, 1642, 1795, 1831
BmsI	GCATC	3 cut(s)	647, 1015, 1916
BmtI	GCTAGC	1 cut(s)	585
BplI	GAGNNNNNCTC	2 cut(s)	766, 798
Bpu10I	CCTNAGC	1 cut(s)	1146
BpuEI	CTTGAG	2 cut(s)	668, 1191
BpuMI	CCSGG	2 cut(s)	308, 1504
BsaHI	GRCGYC	2 cut(s)	32, 278
BsaI	GGTCTC	1 cut(s)	25
BsaJI	CCNNGG	5 cut(s)	24, 807, 1640, 1794, 1829
BsaXI	ACNNNNNCTCC	2 cut(s)	1302, 1332
Bsc4I	CCNNNNNNNGG	5 cut(s)	82, 843, 1509, 1654, 1830
Bse118I	RCCGGY	2 cut(s)	160, 241
Bse1I	ACTGG	1 cut(s)	925
BseBI	CCWGG	3 cut(s)	1642, 1795, 1831
BseDI	CCNNGG	5 cut(s)	24, 807, 1640, 1794, 1829
BseGI	GGATG	5 cut(s)	662, 937, 1083, 1199, 1854
BseLI	CCNNNNNNNGG	5 cut(s)	82, 843, 1509, 1654, 1830
BseMII	CTCAG	3 cut(s)	1010, 1428, 1463
BseNI	ACTGG	1 cut(s)	925
BseRI	GAGGAG	1 cut(s)	1823
BseYI	CCCAGC	1 cut(s)	1780
Bsh1236I	CGCG	3 cut(s)	92, 809, 1478
BshFI	GGCC	6 cut(s)	798, 812, 836, 848, 986, 1333
BshNI	GGYRCC	4 cut(s)	31, 277, 423, 1644
BsiHKAI	GWGCWC	2 cut(s)	784, 1115
BsiHKCI	CYCGRG	1 cut(s)	1536
BsiSI	CCGG	5 cut(s)	161, 242, 275, 308, 1503
BslFI	GGGAC	3 cut(s)	883, 1324, 1811
BslI	CCNNNNNNNGG	5 cut(s)	82, 843, 1509, 1654, 1830
BsmAI	GTCTC	2 cut(s)	25, 1169
BsmFI	GGGAC	3 cut(s)	883, 1324, 1811
BsnI	GGCC	6 cut(s)	798, 812, 836, 848, 986, 1333
Bso31I	GGTCTC	1 cut(s)	25
BsoBI	CYCGRG	1 cut(s)	1536
Bsp1286I	GDGCHC	2 cut(s)	784, 1115
Bsp143I	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
BspANI	GGCC	6 cut(s)	798, 812, 836, 848, 986, 1333
BspCNI	CTCAG	3 cut(s)	1009, 1429, 1462
BspFNI	CGCG	3 cut(s)	92, 809, 1478
BspHI	TCATGA	1 cut(s)	790
BspLI	GGNNCC	8 cut(s)	33, 279, 425, 847, 856, 871, 872, 1646
BspOI	GCTAGC	1 cut(s)	585
BspPI	GGATC	3 cut(s)	1113, 1309, 1773
BspQI	GCTCTTC	1 cut(s)	772
BspT107I	GGYRCC	4 cut(s)	31, 277, 423, 1644
BspTNI	GGTCTC	1 cut(s)	25
BsrFI	RCCGGY	2 cut(s)	160, 241
BsrI	ACTGG	1 cut(s)	925
BssAI	RCCGGY	2 cut(s)	160, 241
BssECI	CCNNGG	5 cut(s)	24, 807, 1640, 1794, 1829
BssMI	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
BssNI	GRCGYC	2 cut(s)	32, 278
BssSI	CACGAG	1 cut(s)	600
Bst2BI	CACGAG	1 cut(s)	600
Bst2UI	CCWGG	3 cut(s)	1642, 1795, 1831
Bst6I	CTCTTC	3 cut(s)	765, 772, 994
BstACI	GRCGYC	2 cut(s)	32, 278
BstAPI	GCANNNNNTGC	2 cut(s)	310, 747
BstC8I	GCNNGC	5 cut(s)	88, 204, 243, 285, 583
BstDEI	CTNAG	5 cut(s)	971, 996, 1146, 1437, 1449
BstDSI	CCRYGG	1 cut(s)	807
BstENI	CCTNNNNNAGG	1 cut(s)	1828
BstF5I	GGATG	5 cut(s)	662, 937, 1083, 1199, 1854
BstFNI	CGCG	3 cut(s)	92, 809, 1478
BstH2I	RGCGCY	2 cut(s)	35, 281
BstHHI	GCGC	4 cut(s)	34, 92, 139, 280
BstKTI	GATC	5 cut(s)	1067, 1108, 1304, 1750, 1768
BstMAI	GTCTC	2 cut(s)	25, 1169
BstMBI	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
BstNI	CCWGG	3 cut(s)	1642, 1795, 1831
BstNSI	RCATGY	1 cut(s)	820
BstSCI	CCNGG	5 cut(s)	306, 1502, 1640, 1793, 1829
BstSFI	CTRYAG	3 cut(s)	113, 119, 1864
BstUI	CGCG	3 cut(s)	92, 809, 1478
BstX2I	RGATCY	3 cut(s)	1064, 1105, 1765
BstYI	RGATCY	3 cut(s)	1064, 1105, 1765
BsuRI	GGCC	6 cut(s)	798, 812, 836, 848, 986, 1333
BtgI	CCRYGG	1 cut(s)	807
BtgZI	GCGATG	1 cut(s)	381
BtsCI	GGATG	5 cut(s)	662, 937, 1083, 1199, 1854
BtsI	GCAGTG	1 cut(s)	407
BtsIMutI	CAGTG	2 cut(s)	407, 1632
Cac8I	GCNNGC	5 cut(s)	88, 204, 243, 285, 583
CciI	TCATGA	1 cut(s)	790
CfoI	GCGC	4 cut(s)	34, 92, 139, 280
Cfr10I	RCCGGY	2 cut(s)	160, 241
Cfr13I	GGNCC	2 cut(s)	846, 870
Cfr42I	CCGCGG	1 cut(s)	810
CseI	GACGC	2 cut(s)	69, 536
CviAII	CATG	4 cut(s)	742, 791, 817, 1235
DdeI	CTNAG	5 cut(s)	971, 996, 1146, 1437, 1449
DinI	GGCGCC	2 cut(s)	33, 279
DpnI	GATC	5 cut(s)	1066, 1107, 1303, 1749, 1767
DpnII	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
DraIII	CACNNNGTG	1 cut(s)	310
EaeI	YGGCCR	1 cut(s)	834
Eam1104I	CTCTTC	3 cut(s)	765, 772, 994
EarI	CTCTTC	3 cut(s)	765, 772, 994
EciI	GGCGGA	2 cut(s)	283, 1091
Ecl136II	GAGCTC	1 cut(s)	782
Eco24I	GRGCYC	1 cut(s)	784
Eco31I	GGTCTC	1 cut(s)	25
Eco32I	GATATC	1 cut(s)	1081
Eco47I	GGWCC	1 cut(s)	870
Eco53kI	GAGCTC	1 cut(s)	782
Eco57I	CTGAAG	2 cut(s)	449, 1697
Eco88I	CYCGRG	1 cut(s)	1536
EcoICRI	GAGCTC	1 cut(s)	782
EcoNI	CCTNNNNNAGG	1 cut(s)	1828
EcoO109I	RGGNCCY	1 cut(s)	870
EcoRII	CCWGG	3 cut(s)	1640, 1793, 1829
EcoRV	GATATC	1 cut(s)	1081
EcoT22I	ATGCAT	1 cut(s)	743
EcoT38I	GRGCYC	1 cut(s)	784
EgeI	GGCGCC	2 cut(s)	33, 279
EheI	GGCGCC	2 cut(s)	33, 279
FaeI	CATG	4 cut(s)	745, 794, 820, 1238
FaqI	GGGAC	3 cut(s)	883, 1324, 1811
FatI	CATG	4 cut(s)	741, 790, 816, 1234
FauI	CCCGC	2 cut(s)	292, 1405
FauNDI	CATATG	1 cut(s)	981
FblI	GTMKAC	1 cut(s)	1659
FokI	GGATG	5 cut(s)	669, 924, 1070, 1186, 1841
FriOI	GRGCYC	1 cut(s)	784
FspBI	CTAG	4 cut(s)	519, 582, 1188, 1814
GlaI	GCGC	4 cut(s)	33, 91, 138, 279
GsaI	CCCAGC	1 cut(s)	1784
HaeII	RGCGCY	2 cut(s)	35, 281
HaeIII	GGCC	6 cut(s)	798, 812, 836, 848, 986, 1333
HapII	CCGG	5 cut(s)	161, 242, 275, 308, 1503
HgaI	GACGC	2 cut(s)	69, 536
HhaI	GCGC	4 cut(s)	34, 92, 139, 280
Hin1I	GRCGYC	2 cut(s)	32, 278
Hin1II	CATG	4 cut(s)	745, 794, 820, 1238
Hin6I	GCGC	4 cut(s)	32, 90, 137, 278
HinP1I	GCGC	4 cut(s)	32, 90, 137, 278
HincII	GTYRAC	2 cut(s)	99, 231
HindII	GTYRAC	2 cut(s)	99, 231
HindIII	AAGCTT	1 cut(s)	317
HinfI	GANTC	6 cut(s)	247, 271, 298, 341, 596, 1601
HpaI	GTTAAC	1 cut(s)	231
HpaII	CCGG	5 cut(s)	161, 242, 275, 308, 1503
HphI	GGTGA	2 cut(s)	150, 1751
Hpy166II	GTNNAC	6 cut(s)	73, 99, 231, 505, 1267, 1660
Hpy188I	TCNGA	4 cut(s)	25, 297, 727, 1935
Hpy188III	TCNNGA	9 cut(s)	64, 602, 685, 791, 885, 1208, 1299, 1451, 1498
Hpy8I	GTNNAC	6 cut(s)	73, 99, 231, 505, 1267, 1660
Hpy99I	CGWCG	2 cut(s)	70, 85
HpyAV	CCTTC	3 cut(s)	755, 788, 1053
HpyCH4IV	ACGT	4 cut(s)	105, 291, 361, 1117
HpyF3I	CTNAG	5 cut(s)	971, 996, 1146, 1437, 1449
HpySE526I	ACGT	4 cut(s)	105, 291, 361, 1117
Hsp92I	GRCGYC	2 cut(s)	32, 278
Hsp92II	CATG	4 cut(s)	745, 794, 820, 1238
HspAI	GCGC	4 cut(s)	32, 90, 137, 278
KasI	GGCGCC	2 cut(s)	31, 277
KflI	GGGWCCC	1 cut(s)	870
KpnI	GGTACC	1 cut(s)	1648
KroI	GCCGGC	1 cut(s)	241
KroNI	GCCGGC	1 cut(s)	243
KspAI	GTTAAC	1 cut(s)	231
KspI	CCGCGG	1 cut(s)	810
Kzo9I	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
LguI	GCTCTTC	1 cut(s)	772
LmnI	GCTCC	6 cut(s)	4, 536, 1136, 1542, 1810, 1896
LweI	GCATC	3 cut(s)	647, 1015, 1916
MaeI	CTAG	4 cut(s)	519, 582, 1188, 1814
MaeII	ACGT	4 cut(s)	105, 291, 361, 1117
MaeIII	GTNAC	5 cut(s)	129, 491, 1345, 1729, 1739
MalI	GATC	5 cut(s)	1066, 1107, 1303, 1749, 1767
MboI	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
MboII	GAAGA	9 cut(s)	49, 626, 782, 789, 981, 984, 1229, 1703, 1902
MfeI	CAATTG	3 cut(s)	53, 406, 942
MflI	RGATCY	3 cut(s)	1064, 1105, 1765
MhlI	GDGCHC	2 cut(s)	784, 1115
Mly113I	GGCGCC	2 cut(s)	32, 278
MlyI	GAGTC	2 cut(s)	280, 335
MmeI	TCCRAC	4 cut(s)	592, 1111, 1594, 1743
Mph1103I	ATGCAT	1 cut(s)	743
MreI	CGCCGGCG	1 cut(s)	241
MroNI	GCCGGC	1 cut(s)	241
MseI	TTAA	3 cut(s)	230, 1521, 1589
MslI	CAYNNNNRTG	2 cut(s)	68, 498
MspA1I	CMGCKG	4 cut(s)	455, 809, 1031, 1142
MspI	CCGG	5 cut(s)	161, 242, 275, 308, 1503
MspR9I	CCNGG	5 cut(s)	308, 1504, 1642, 1795, 1831
MunI	CAATTG	3 cut(s)	53, 406, 942
MvaI	CCWGG	3 cut(s)	1642, 1795, 1831
MvnI	CGCG	3 cut(s)	92, 809, 1478
NaeI	GCCGGC	1 cut(s)	243
NarI	GGCGCC	2 cut(s)	32, 278
NciI	CCSGG	2 cut(s)	308, 1504
NdeI	CATATG	1 cut(s)	981
NdeII	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
NgoMIV	GCCGGC	1 cut(s)	241
NheI	GCTAGC	1 cut(s)	581
NlaIII	CATG	4 cut(s)	745, 794, 820, 1238
NlaIV	GGNNCC	8 cut(s)	33, 279, 425, 847, 856, 871, 872, 1646
NmeAIII	GCCGAG	1 cut(s)	244
NmuCI	GTSAC	4 cut(s)	129, 491, 1729, 1739
NsiI	ATGCAT	1 cut(s)	743
NspI	RCATGY	1 cut(s)	820
OliI	CACNNNNGTG	1 cut(s)	68
PagI	TCATGA	1 cut(s)	790
PciSI	GCTCTTC	1 cut(s)	772
PdiI	GCCGGC	1 cut(s)	243
PfeI	GAWTC	4 cut(s)	247, 298, 596, 1601
PflFI	GACNNNGTC	1 cut(s)	127
PleI	GAGTC	2 cut(s)	279, 335
PluTI	GGCGCC	2 cut(s)	35, 281
PpsI	GAGTC	2 cut(s)	279, 335
PpuMI	RGGWCCY	1 cut(s)	870
Psp124BI	GAGCTC	1 cut(s)	784
Psp1406I	AACGTT	1 cut(s)	291
Psp5II	RGGWCCY	1 cut(s)	870
Psp6I	CCWGG	3 cut(s)	1640, 1793, 1829
PspFI	CCCAGC	1 cut(s)	1780
PspGI	CCWGG	3 cut(s)	1640, 1793, 1829
PspN4I	GGNNCC	8 cut(s)	33, 279, 425, 847, 856, 871, 872, 1646
PspPI	GGNCC	2 cut(s)	846, 870
PspPPI	RGGWCCY	1 cut(s)	870
PsuI	RGATCY	3 cut(s)	1064, 1105, 1765
PsyI	GACNNNGTC	1 cut(s)	127
PvuII	CAGCTG	3 cut(s)	455, 1031, 1142
RseI	CAYNNNNRTG	2 cut(s)	68, 498
SacI	GAGCTC	1 cut(s)	784
SacII	CCGCGG	1 cut(s)	810
SapI	GCTCTTC	1 cut(s)	772
SaqAI	TTAA	3 cut(s)	230, 1521, 1589
Sau3AI	GATC	5 cut(s)	1064, 1105, 1301, 1747, 1765
Sau96I	GGNCC	2 cut(s)	846, 870
SchI	GAGTC	2 cut(s)	280, 335
ScrFI	CCNGG	5 cut(s)	308, 1504, 1642, 1795, 1831
SduI	GDGCHC	2 cut(s)	784, 1115
SfaNI	GCATC	3 cut(s)	647, 1015, 1916
SfcI	CTRYAG	3 cut(s)	113, 119, 1864
SfoI	GGCGCC	2 cut(s)	33, 279
Sfr303I	CCGCGG	1 cut(s)	810
SgrAI	CRCCGGYG	1 cut(s)	241
SgrBI	CCGCGG	1 cut(s)	810
SinI	GGWCC	1 cut(s)	870
SmiMI	CAYNNNNRTG	2 cut(s)	68, 498
SmlI	CTYRAG	2 cut(s)	683, 1206
SmoI	CTYRAG	2 cut(s)	683, 1206
SpeI	ACTAGT	1 cut(s)	1187
SspDI	GGCGCC	2 cut(s)	31, 277
SspI	AATATT	1 cut(s)	715
SspMI	CTAG	4 cut(s)	519, 582, 1188, 1814
SstI	GAGCTC	1 cut(s)	784
StyD4I	CCNGG	5 cut(s)	306, 1502, 1640, 1793, 1829
TaiI	ACGT	4 cut(s)	108, 294, 364, 1120
TaqI	TCGA	2 cut(s)	221, 1879
TatI	WGTACW	2 cut(s)	380, 435
TauI	GCSGC	9 cut(s)	95, 199, 209, 238, 241, 283, 638, 812, 836
TfiI	GAWTC	4 cut(s)	247, 298, 596, 1601
Tru1I	TTAA	3 cut(s)	230, 1521, 1589
Tru9I	TTAA	3 cut(s)	230, 1521, 1589
TscAI	CASTG	2 cut(s)	407, 1639
TseFI	GTSAC	4 cut(s)	129, 491, 1729, 1739
Tsp45I	GTSAC	4 cut(s)	129, 491, 1729, 1739
TspDTI	ATGAA	7 cut(s)	384, 525, 807, 918, 1188, 1383, 1680
TspGWI	ACGGA	3 cut(s)	116, 1594, 1595
TspRI	CASTG	2 cut(s)	407, 1639
Tth111I	GACNNNGTC	1 cut(s)	127
VpaK11BI	GGWCC	1 cut(s)	870
XagI	CCTNNNNNAGG	1 cut(s)	1828
XapI	RAATTY	3 cut(s)	459, 627, 1707
XceI	RCATGY	1 cut(s)	820
XmiI	GTMKAC	1 cut(s)	1659
XspI	CTAG	4 cut(s)	519, 582, 1188, 1814
Zsp2I	ATGCAT	1 cut(s)	743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.