MD08G1117100.v1.1

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009536
Physical Location & Seq
Forward (+)
10716032 .. 10727885
11854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1117100.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGCCGCATACGACTACGACGACGCCGGCACCTACGACGAGACATCGGCGACGCAGCGCCAACCGTCGTCCGGAGCCCCGCCGTCTTCCGACCTCGGTTACGACCCCAACTTCGTCCCCGACTCCGTCAAATCCTTTGTCGTCCACCTCTACCGCTACATCCGCGAGAAGAATGTCTACGAGATCCACCAGATGTACGAGACCTCCTTCCAGACCCTCTCCGATCGCCTCTTCAAGGACACCCCCTGGCCATCCGTCGATGCCGTCGCCGACTACGTCGACAACGACCACGTATTCTGCCTCCTTTACCGCGAGATGTGGTTCCGCCACCTCTACGCCCGCCTGTCCCCGACGCTGAAGCAGCGAATCGACTCCTGGGACAATTACTGCAGCCTCTTCCAGGTTGTCCTCCACGGCGTCGTTAATATGCAGCTGCCGAACCAGTGGCTTTGGGACATGGTCGATGAGTTCGTCTACCAGTTCCAGAGCTTCTGTCAGTACAGGGCTAAGATGAAGAACAAGAACGAGCAGGAGATTGCTCTTCTCAGGCAATTTGATCAAGCTTGGAATGTGTATGGTGTGCTCAATTTCTTGCAAGCTCTGGTTGAGAAGTCGATGATCATTCAGATTCTCGAGCAAGAGAAGGAAGGGCTCGAGCAGTTTACTGCCAATGATGGGTATGATTATAGTGGTGGCAGTAATGTGTTGAAGGTGTTGGGGTACTTCAGCATGGTGGGTTTGCTTCGAGTTCATTGTCTTTTGGGCGATTATCATACCGGTCTGAAATGCTTGCAGCCCATCGACATTACCCAACAAGGTGTTTTCACCACTGTTATTGGAAGCCACATTACCACCATTTATCATTATGGTTTCGCCAATCTTATGTTGCGGAGGTACGTGGAGGCAATTCGTGAATTCAATAAGATTCTGTTGTACATCTACAAGACCAAGCAGTTCCATCAGAAATCTCCGCAGTATGAGCAGATACTAAAGAAGAATGAGCAGATGTATGCCCTGTTGGCAATTGCTCTTTCACTTTGTCCCCAAGTGAAGCTTGTTGAGGAAACTGTGAACTCTCAGTTGCGCGAGAAGTATGGTGAGAAGATGAACAGAATGCAAAGATATGATGATGAAGCATTTGCTATTTATGATGAGCTCTTCTCCTATGCATGCCCTAAGTTCATTACCCCATCTCCTCCAAGTTTTGAGGAGCCCCTTGTTAATTACAACCAGGATGCTTATAGACTTCAATTGAAACTGTTTCTTTATGAAGTGAAGCAGCAACAGTTATTATCAGGCGTCAGGACCTTCTTAAAAGTCTATTCATCCATCTCCGTTGGAAAGCTTGCAAGCTACATGGAAGTGGATGAAGCCAAGTTGAGGACTATCTTGTTGACGTACAAGCACAAGACACATTCCGTTGATACTGAGGGAAAAGTTATTTCCAATGCTGATGTGGACTTCTATATTGACGATGACTTGATTTCCGTCGTCGAATCGAAACCAGCAAAACGATACGGTGATTACTTCTTGCGGCAGATCGTGAAGCTTGAAGGTGTGATCAATGATATGGACAGGGTAAAGCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Paf67

Protein Analysis

531

Amino Acids

61.97

Weight (kDa)

5.57

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Paf67 PF10255 121 - 516 7.1e-153 RNA polymerase I-associated factor PAF67
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AccI GTMKAC 3 cut(s) 177, 279, 474
AccII CGCG 3 cut(s) 165, 312, 1086
AccIII TCCGGA 1 cut(s) 71
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 2 cut(s) 3, 248
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 2 cut(s) 377, 709
AcyI GRCGYC 3 cut(s) 24, 417, 1299
AfaI GTAC 6 cut(s) 197, 500, 722, 896, 935, 1400
AfiI CCNNNNNNNGG 5 cut(s) 71, 235, 400, 675, 1380
AgeI ACCGGT 1 cut(s) 776
AgsI TTSAA 6 cut(s) 235, 709, 919, 1250, 1255, 1553
AjnI CCWGG 4 cut(s) 245, 374, 399, 1230
Alw21I GWGCWC 2 cut(s) 585, 1158
Alw26I GTCTC 2 cut(s) 35, 194
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 2 cut(s) 632, 653
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 2 cut(s) 3, 248
ApeKI GCWGC 7 cut(s) 55, 361, 390, 430, 433, 793, 1279
ApoI RAATTY 1 cut(s) 914
ArsI GACNNNNNNTTYG 4 cut(s) 95, 123, 127, 155
AsiGI ACCGGT 1 cut(s) 776
AspLEI GCGC 2 cut(s) 60, 1086
AspS9I GGNCC 1 cut(s) 1305
AsuHPI GGTGA 3 cut(s) 817, 1109, 1532
AvaI CYCGRG 2 cut(s) 632, 653
AvaII GGWCC 1 cut(s) 1305
BalI TGGCCA 1 cut(s) 250
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 4 cut(s) 79, 654, 1158, 1215
BarI GAAGNNNNNNTAC 4 cut(s) 161, 193, 832, 864
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 585, 1158
BbvI GCAGC 7 cut(s) 67, 373, 402, 420, 442, 805, 1291
BccI CCATC 6 cut(s) 259, 668, 806, 966, 1198, 1337
BceAI ACGGC 3 cut(s) 67, 248, 430
BciT130I CCWGG 4 cut(s) 247, 376, 401, 1232
BclI TGATCA 3 cut(s) 556, 618, 1560
BcoDI GTCTC 2 cut(s) 35, 194
BfmI CTRYAG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 61
BglI GCCNNNNNGGC 1 cut(s) 1019
BisI GCNGC 9 cut(s) 6, 56, 362, 391, 431, 434, 794, 1280, 1535
BlsI GCNGC 9 cut(s) 7, 57, 363, 392, 432, 435, 795, 1281, 1536
Bme1390I CCNGG 4 cut(s) 247, 376, 401, 1232
Bme18I GGWCC 1 cut(s) 1305
BmeT110I CYCGRG 2 cut(s) 632, 653
BmgT120I GGNCC 1 cut(s) 1305
BmiI GGNNCC 4 cut(s) 31, 76, 323, 1212
BmrFI CCNGG 4 cut(s) 247, 376, 401, 1232
BmsI GCATC 2 cut(s) 250, 1225
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 1145, 1177
BsaAI YACGTR 2 cut(s) 292, 898
BsaHI GRCGYC 3 cut(s) 24, 417, 1299
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 94, 245, 375, 412
BsaWI WCCGGW 2 cut(s) 71, 776
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 1177, 1207
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 235, 400, 675, 1380
Bse118I RCCGGY 2 cut(s) 26, 776
Bse1I ACTGG 2 cut(s) 442, 478
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 4 cut(s) 247, 376, 401, 1232
BseDI CCNNGG 4 cut(s) 94, 245, 375, 412
BseGI GGATG 5 cut(s) 159, 251, 1240, 1325, 1372
BseLI CCNNNNNNNGG 5 cut(s) 71, 235, 400, 675, 1380
BseMII CTCAG 3 cut(s) 559, 1091, 1419
BseNI ACTGG 2 cut(s) 442, 478
BseRI GAGGAG 2 cut(s) 1185, 1223
BseXI GCAGC 7 cut(s) 67, 373, 402, 420, 442, 805, 1291
Bsh1236I CGCG 3 cut(s) 165, 312, 1086
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 5, 250
BshNI GGYRCC 1 cut(s) 29
BshTI ACCGGT 1 cut(s) 776
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 2 cut(s) 585, 1158
BsiHKCI CYCGRG 2 cut(s) 632, 653
BsiSI CCGG 3 cut(s) 27, 72, 777
BslFI GGGAC 5 cut(s) 101, 331, 392, 467, 1026
BslI CCNNNNNNNGG 5 cut(s) 71, 235, 400, 675, 1380
BsmAI GTCTC 2 cut(s) 35, 194
BsmFI GGGAC 5 cut(s) 101, 331, 392, 467, 1026
BsmI GAATGC 1 cut(s) 1119
BsnI GGCC 2 cut(s) 5, 250
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 2 cut(s) 632, 653
Bsp1286I GDGCHC 5 cut(s) 79, 585, 654, 1158, 1215
Bsp13I TCCGGA 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
BspANI GGCC 2 cut(s) 5, 250
BspCNI CTCAG 3 cut(s) 558, 1090, 1420
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 3 cut(s) 165, 312, 1086
BspLI GGNNCC 4 cut(s) 31, 76, 323, 1212
BspMAI CTGCAG 1 cut(s) 392
BspPI GGATC 1 cut(s) 178
BspQI GCTCTTC 2 cut(s) 546, 1163
BspT107I GGYRCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 2 cut(s) 26, 776
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 2 cut(s) 442, 478
BssAI RCCGGY 2 cut(s) 26, 776
BssECI CCNNGG 4 cut(s) 94, 245, 375, 412
BssMI GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
BssNI GRCGYC 3 cut(s) 24, 417, 1299
Bst2UI CCWGG 4 cut(s) 247, 376, 401, 1232
Bst4CI ACNGT 6 cut(s) 66, 832, 1069, 1260, 1287, 1520
Bst6I CTCTTC 4 cut(s) 236, 401, 546, 1163
BstACI GRCGYC 3 cut(s) 24, 417, 1299
BstAUI TGTACA 1 cut(s) 933
BstBAI YACGTR 2 cut(s) 292, 898
BstC8I GCNNGC 7 cut(s) 28, 340, 597, 791, 1171, 1347, 1351
BstDEI CTNAG 5 cut(s) 507, 545, 1077, 1176, 1428
BstDSI CCRYGG 1 cut(s) 412
BstENI CCTNNNNNAGG 2 cut(s) 233, 398
BstF5I GGATG 5 cut(s) 159, 251, 1240, 1325, 1372
BstFNI CGCG 3 cut(s) 165, 312, 1086
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 2 cut(s) 60, 1086
BstKTI GATC 6 cut(s) 186, 226, 559, 621, 1542, 1563
BstMAI GTCTC 2 cut(s) 35, 194
BstMBI GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 162, 361, 979, 1019
BstNI CCWGG 4 cut(s) 247, 376, 401, 1232
BstNSI RCATGY 1 cut(s) 1173
BstSCI CCNGG 4 cut(s) 245, 374, 399, 1230
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 3 cut(s) 165, 312, 1086
BstV1I GCAGC 7 cut(s) 67, 373, 402, 420, 442, 805, 1291
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 2 cut(s) 5, 250
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 5 cut(s) 159, 251, 1240, 1325, 1372
BtsIMutI CAGTG 2 cut(s) 449, 828
Cac8I GCNNGC 7 cut(s) 28, 340, 597, 791, 1171, 1347, 1351
CfoI GCGC 2 cut(s) 60, 1086
Cfr10I RCCGGY 2 cut(s) 26, 776
Cfr13I GGNCC 1 cut(s) 1305
CseI GACGC 5 cut(s) 32, 61, 361, 406, 1288
Csp6I GTAC 6 cut(s) 196, 499, 721, 895, 934, 1399
CspAI ACCGGT 1 cut(s) 776
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 4 cut(s) 457, 730, 1170, 1357
CviQI GTAC 6 cut(s) 196, 499, 721, 895, 934, 1399
DdeI CTNAG 5 cut(s) 507, 545, 1077, 1176, 1428
DpnI GATC 6 cut(s) 185, 225, 558, 620, 1541, 1562
DpnII GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
EaeI YGGCCR 2 cut(s) 3, 248
Eam1104I CTCTTC 4 cut(s) 236, 401, 546, 1163
EarI CTCTTC 4 cut(s) 236, 401, 546, 1163
EciI GGCGGA 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 1156
Eco24I GRGCYC 4 cut(s) 79, 654, 1158, 1215
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 1305
Eco53kI GAGCTC 1 cut(s) 1156
Eco57I CTGAAG 2 cut(s) 377, 709
Eco88I CYCGRG 2 cut(s) 632, 653
EcoICRI GAGCTC 1 cut(s) 1156
EcoNI CCTNNNNNAGG 2 cut(s) 233, 398
EcoO109I RGGNCCY 1 cut(s) 1305
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 4 cut(s) 245, 374, 399, 1230
EcoT22I ATGCAT 1 cut(s) 1171
EcoT38I GRGCYC 4 cut(s) 79, 654, 1158, 1215
FaeI CATG 4 cut(s) 460, 733, 1173, 1360
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 5 cut(s) 101, 331, 392, 467, 1026
FatI CATG 4 cut(s) 456, 729, 1169, 1356
FauI CCCGC 2 cut(s) 87, 347
FbaI TGATCA 3 cut(s) 556, 618, 1560
FblI GTMKAC 3 cut(s) 177, 279, 474
Fnu4HI GCNGC 9 cut(s) 6, 56, 362, 391, 431, 434, 794, 1280, 1535
FokI GGATG 5 cut(s) 146, 238, 1247, 1312, 1379
FriOI GRGCYC 4 cut(s) 79, 654, 1158, 1215
Fsp4HI GCNGC 9 cut(s) 6, 56, 362, 391, 431, 434, 794, 1280, 1535
GlaI GCGC 2 cut(s) 59, 1085
GluI GCNGC 9 cut(s) 6, 56, 362, 391, 431, 434, 794, 1280, 1535
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 2 cut(s) 5, 250
HapII CCGG 3 cut(s) 27, 72, 777
HgaI GACGC 5 cut(s) 32, 61, 361, 406, 1288
HhaI GCGC 2 cut(s) 60, 1086
Hin1I GRCGYC 3 cut(s) 24, 417, 1299
Hin1II CATG 4 cut(s) 460, 733, 1173, 1360
Hin6I GCGC 2 cut(s) 58, 1084
HinP1I GCGC 2 cut(s) 58, 1084
HincII GTYRAC 2 cut(s) 280, 1395
HindII GTYRAC 2 cut(s) 280, 1395
HindIII AAGCTT 5 cut(s) 561, 1052, 1343, 1547, 1583
HinfI GANTC 6 cut(s) 122, 366, 371, 628, 925, 1496
HpaII CCGG 3 cut(s) 27, 72, 777
HphI GGTGA 3 cut(s) 817, 1109, 1532
Hpy166II GTNNAC 8 cut(s) 145, 178, 280, 475, 663, 1072, 1395, 1459
Hpy188I TCNGA 5 cut(s) 91, 223, 627, 783, 963
Hpy188III TCNNGA 7 cut(s) 72, 211, 484, 632, 911, 1303, 1543
Hpy8I GTNNAC 8 cut(s) 145, 178, 280, 475, 663, 1072, 1395, 1459
HpyAV CCTTC 6 cut(s) 217, 637, 641, 703, 1318, 1547
HpyCH4III ACNGT 6 cut(s) 66, 832, 1069, 1260, 1287, 1520
HpyCH4IV ACGT 4 cut(s) 276, 291, 897, 1397
HpyCH4V TGCA 7 cut(s) 390, 430, 595, 793, 1117, 1169, 1349
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 361, 979, 1019
HpyF3I CTNAG 5 cut(s) 507, 545, 1077, 1176, 1428
HpySE526I ACGT 4 cut(s) 276, 291, 897, 1397
Hsp92I GRCGYC 3 cut(s) 24, 417, 1299
Hsp92II CATG 4 cut(s) 460, 733, 1173, 1360
HspAI GCGC 2 cut(s) 58, 1084
Kpn2I TCCGGA 1 cut(s) 71
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Ksp22I TGATCA 3 cut(s) 556, 618, 1560
Kzo9I GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
LguI GCTCTTC 2 cut(s) 546, 1163
LmnI GCTCC 2 cut(s) 74, 1210
Lsp1109I GCAGC 7 cut(s) 67, 373, 402, 420, 442, 805, 1291
LweI GCATC 2 cut(s) 250, 1225
MaeII ACGT 4 cut(s) 276, 291, 897, 1397
MaeIII GTNAC 1 cut(s) 98
MalI GATC 6 cut(s) 185, 225, 558, 620, 1541, 1562
MboI GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
MboII GAAGA 9 cut(s) 78, 181, 223, 388, 526, 533, 1006, 1114, 1150
MfeI CAATTG 2 cut(s) 1023, 1250
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 5 cut(s) 79, 585, 654, 1158, 1215
MlsI TGGCCA 1 cut(s) 250
MluCI AATT 8 cut(s) 382, 551, 586, 906, 914, 1023, 1222, 1250
MluNI TGGCCA 1 cut(s) 250
MlyI GAGTC 2 cut(s) 116, 365
MmeI TCCRAC 2 cut(s) 114, 1318
Mox20I TGGCCA 1 cut(s) 250
Mph1103I ATGCAT 1 cut(s) 1171
MroI TCCGGA 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 26
MscI TGGCCA 1 cut(s) 250
MseI TTAA 3 cut(s) 423, 1221, 1313
MslI CAYNNNNRTG 1 cut(s) 1361
Msp20I TGGCCA 1 cut(s) 250
MspA1I CMGCKG 1 cut(s) 433
MspI CCGG 3 cut(s) 27, 72, 777
MspR9I CCNGG 4 cut(s) 247, 376, 401, 1232
MunI CAATTG 2 cut(s) 1023, 1250
Mva1269I GAATGC 1 cut(s) 1119
MvaI CCWGG 4 cut(s) 247, 376, 401, 1232
MvnI CGCG 3 cut(s) 165, 312, 1086
MwoI GCNNNNNNNGC 4 cut(s) 162, 361, 979, 1019
NaeI GCCGGC 1 cut(s) 28
NdeII GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 4 cut(s) 460, 733, 1173, 1360
NlaIV GGNNCC 4 cut(s) 31, 76, 323, 1212
NsiI ATGCAT 1 cut(s) 1171
NspI RCATGY 1 cut(s) 1173
PaeI GCATGC 1 cut(s) 1173
PaeR7I CTCGAG 2 cut(s) 632, 653
PciSI GCTCTTC 2 cut(s) 546, 1163
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 273, 282, 468
PctI GAATGC 1 cut(s) 1119
PdiI GCCGGC 1 cut(s) 28
PfeI GAWTC 4 cut(s) 366, 628, 925, 1496
PflFI GACNNNGTC 3 cut(s) 125, 275, 458
PinAI ACCGGT 1 cut(s) 776
PkrI GCNGC 9 cut(s) 7, 57, 363, 392, 432, 435, 795, 1281, 1536
Ple19I CGATCG 1 cut(s) 226
PleI GAGTC 2 cut(s) 116, 365
PpsI GAGTC 2 cut(s) 116, 365
Ppu21I YACGTR 2 cut(s) 292, 898
PpuMI RGGWCCY 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 1305
Psp6I CCWGG 4 cut(s) 245, 374, 399, 1230
PspGI CCWGG 4 cut(s) 245, 374, 399, 1230
PspN4I GGNNCC 4 cut(s) 31, 76, 323, 1212
PspPI GGNCC 1 cut(s) 1305
PspPPI RGGWCCY 1 cut(s) 1305
PspXI VCTCGAGB 1 cut(s) 653
PstI CTGCAG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 183
PsyI GACNNNGTC 3 cut(s) 125, 275, 458
PvuI CGATCG 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 433
RsaI GTAC 6 cut(s) 197, 500, 722, 896, 935, 1400
RsaNI GTAC 6 cut(s) 196, 499, 721, 895, 934, 1399
RseI CAYNNNNRTG 1 cut(s) 1361
SacI GAGCTC 1 cut(s) 1158
SalI GTCGAC 1 cut(s) 278
SapI GCTCTTC 2 cut(s) 546, 1163
SaqAI TTAA 3 cut(s) 423, 1221, 1313
SatI GCNGC 9 cut(s) 6, 56, 362, 391, 431, 434, 794, 1280, 1535
Sau3AI GATC 6 cut(s) 183, 223, 556, 618, 1539, 1560
Sau96I GGNCC 1 cut(s) 1305
SchI GAGTC 2 cut(s) 116, 365
ScrFI CCNGG 4 cut(s) 247, 376, 401, 1232
SduI GDGCHC 5 cut(s) 79, 585, 654, 1158, 1215
SfaNI GCATC 2 cut(s) 250, 1225
SfcI CTRYAG 1 cut(s) 388
Sfr274I CTCGAG 2 cut(s) 632, 653
SinI GGWCC 1 cut(s) 1305
SlaI CTCGAG 2 cut(s) 632, 653
SmiMI CAYNNNNRTG 1 cut(s) 1361
SmlI CTYRAG 2 cut(s) 632, 653
SmoI CTYRAG 2 cut(s) 632, 653
SphI GCATGC 1 cut(s) 1173
Sse9I AATT 8 cut(s) 382, 551, 586, 906, 914, 1023, 1222, 1250
SstI GAGCTC 1 cut(s) 1158
StyD4I CCNGG 4 cut(s) 245, 374, 399, 1230
TaaI ACNGT 6 cut(s) 66, 832, 1069, 1260, 1287, 1520
TaiI ACGT 4 cut(s) 279, 294, 900, 1400
TasI AATT 8 cut(s) 382, 551, 586, 906, 914, 1023, 1222, 1250
TatI WGTACW 2 cut(s) 498, 933
TauI GCSGC 2 cut(s) 8, 1537
TfiI GAWTC 4 cut(s) 366, 628, 925, 1496
Tru1I TTAA 3 cut(s) 423, 1221, 1313
Tru9I TTAA 3 cut(s) 423, 1221, 1313
TscAI CASTG 2 cut(s) 449, 835
TseI GCWGC 7 cut(s) 55, 361, 390, 430, 433, 793, 1279
TspDTI ATGAA 8 cut(s) 527, 740, 1121, 1146, 1171, 1284, 1314, 1383
TspGWI ACGGA 5 cut(s) 115, 244, 1324, 1408, 1477
TspRI CASTG 2 cut(s) 449, 835
Tth111I GACNNNGTC 3 cut(s) 125, 275, 458
VpaK11BI GGWCC 1 cut(s) 1305
XagI CCTNNNNNAGG 2 cut(s) 233, 398
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 1173
XcmI CCANNNNNNNNNTGG 1 cut(s) 1453
XhoI CTCGAG 2 cut(s) 632, 653
XmiI GTMKAC 3 cut(s) 177, 279, 474
Zsp2I ATGCAT 1 cut(s) 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.