pycom08g09770

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009536
Physical Location & Seq
Forward (+)
8122922 .. 8125131
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09770.4

Sequence Viewer

Length: 864 bp
ATGGTGGGTTTGCTTCGAGTTCATTGTCTTTTGGGTGATTATCATACCGGTCTGAAATGCTTGCAGCCCATCGACATTACCCAACAAGGTGTTTTCACCACTGTTATTGGAAGCCACATTACCACCATTTATCATTATGGTTTCGCCAATCTTATGTTGCGGAGGTACGTGGAGGCGATTCGTGAATTCAATAAGATTCTGTTGTACATCTACAAGACCAAGCAGTTCCATCAGAAATCTCCTCAGTATGAGCAGATACTAAAAAAGAATGAGCAGATGTATGCCCTGTTGGCAATTGCTCTTTCACTATGTCCCCAAGTGAAGCTTGTTGAGGAAACTGTGAACTCTCAGTTGCGCGAGAAGTATGGTGAGAAGATGAACAGAATGCAAAGATATGATGATGAAGCATTTGCTATTTACGATGAGCTCTTCTCCTATGCATGCCCTAAGTTCATTACCCCATCTCCTCCAAGTTTTGAGGAGCCCCTTGTTAATTACAACCAGGATGCTTATAGACTTCAATTGAAACTGTTCCTTTATGAAGTGAAGCAGCAACAGTTATTATCAGGCGTCAGGACCTTCTTAAAAGTCTATTCATCCATCTCCGTTGGAAAGCTTGCAAGCTACATGGAAGTGGATGAAGCCAAGTTGAGGACTATCTTGTTGACATACAAGCACAAGACACATTCCGTTGATACTGAGGGAAAAGTTATTTCCAATGCTGATGTGGACTTCTATATTGACGATGACTTGATTTCTGTCGTCGAATCAAAACCAGTAAAACGATACGGTGATTACTTCTTGCGGCAGATCGTGAAGCTTGAAGGTGTGATCAATGATATGGACAGGGTAAAGCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Paf67

Protein Analysis

288

Amino Acids

33.46

Weight (kDa)

7.64

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 357
AciI CCGC 2 cut(s) 160, 805
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 2 cut(s) 167, 206
AfiI CCNNNNNNNGG 1 cut(s) 651
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 4 cut(s) 190, 521, 526, 824
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 6 cut(s) 325, 427, 616, 624, 820, 856
AluI AGCT 6 cut(s) 325, 427, 616, 624, 820, 856
Alw21I GWGCWC 1 cut(s) 429
ApeKI GCWGC 2 cut(s) 64, 550
ApoI RAATTY 1 cut(s) 185
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 576
AsuHPI GGTGA 4 cut(s) 47, 88, 380, 803
AvaII GGWCC 1 cut(s) 576
BanII GRGCYC 2 cut(s) 429, 486
BarI GAAGNNNNNNTAC 2 cut(s) 103, 135
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 76, 562
BccI CCATC 4 cut(s) 77, 237, 469, 608
BciT130I CCWGG 1 cut(s) 503
BclI TGATCA 1 cut(s) 831
BglI GCCNNNNNGGC 1 cut(s) 290
BisI GCNGC 3 cut(s) 65, 551, 806
BlsI GCNGC 3 cut(s) 66, 552, 807
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 576
BmgT120I GGNCC 1 cut(s) 576
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 1 cut(s) 496
BplI GAGNNNNNCTC 2 cut(s) 416, 448
BsaAI YACGTR 1 cut(s) 169
BsaHI GRCGYC 1 cut(s) 570
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 448, 478
Bsc4I CCNNNNNNNGG 1 cut(s) 651
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 776
BseBI CCWGG 1 cut(s) 503
BseGI GGATG 3 cut(s) 511, 596, 643
BseLI CCNNNNNNNGG 1 cut(s) 651
BseMII CTCAG 3 cut(s) 257, 362, 690
BseNI ACTGG 1 cut(s) 776
BseRI GAGGAG 3 cut(s) 231, 456, 494
BseXI GCAGC 2 cut(s) 76, 562
Bsh1236I CGCG 1 cut(s) 357
BshTI ACCGGT 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 651
BsmFI GGGAC 1 cut(s) 297
BsmI GAATGC 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 429, 486
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 810, 831
BspACI CCGC 2 cut(s) 160, 805
BspCNI CTCAG 3 cut(s) 256, 361, 691
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 483
BspQI GCTCTTC 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 1 cut(s) 776
BssAI RCCGGY 1 cut(s) 47
BssMI GATC 2 cut(s) 810, 831
BssNI GRCGYC 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 5 cut(s) 103, 340, 531, 558, 791
Bst6I CTCTTC 1 cut(s) 434
BstACI GRCGYC 1 cut(s) 570
BstAUI TGTACA 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 4 cut(s) 62, 442, 618, 622
BstDEI CTNAG 4 cut(s) 243, 348, 447, 699
BstF5I GGATG 3 cut(s) 511, 596, 643
BstFNI CGCG 1 cut(s) 357
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 2 cut(s) 813, 834
BstMBI GATC 2 cut(s) 810, 831
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 501
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 76, 562
BtsCI GGATG 3 cut(s) 511, 596, 643
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 62, 442, 618, 622
CfoI GCGC 1 cut(s) 357
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 576
CseI GACGC 1 cut(s) 559
Csp6I GTAC 2 cut(s) 166, 205
CspAI ACCGGT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviAII CATG 2 cut(s) 441, 628
CviQI GTAC 2 cut(s) 166, 205
DdeI CTNAG 4 cut(s) 243, 348, 447, 699
DpnI GATC 2 cut(s) 812, 833
DpnII GATC 2 cut(s) 810, 831
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 429, 486
Eco47I GGWCC 1 cut(s) 576
Eco53kI GAGCTC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 2 cut(s) 429, 486
FaeI CATG 2 cut(s) 444, 631
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 297
FatI CATG 2 cut(s) 440, 627
FbaI TGATCA 1 cut(s) 831
Fnu4HI GCNGC 3 cut(s) 65, 551, 806
FokI GGATG 3 cut(s) 518, 583, 650
FriOI GRGCYC 2 cut(s) 429, 486
Fsp4HI GCNGC 3 cut(s) 65, 551, 806
GlaI GCGC 1 cut(s) 356
GluI GCNGC 3 cut(s) 65, 551, 806
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 559
HhaI GCGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 2 cut(s) 444, 631
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 4 cut(s) 323, 614, 818, 854
HinfI GANTC 3 cut(s) 178, 196, 767
HpaII CCGG 1 cut(s) 48
HphI GGTGA 4 cut(s) 47, 88, 380, 803
Hpy166II GTNNAC 3 cut(s) 343, 666, 730
Hpy188I TCNGA 2 cut(s) 54, 234
Hpy188III TCNNGA 3 cut(s) 182, 574, 814
Hpy8I GTNNAC 3 cut(s) 343, 666, 730
Hpy99I CGWCG 1 cut(s) 767
HpyAV CCTTC 2 cut(s) 589, 818
HpyCH4III ACNGT 5 cut(s) 103, 340, 531, 558, 791
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 4 cut(s) 64, 388, 440, 620
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 4 cut(s) 243, 348, 447, 699
HpySE526I ACGT 1 cut(s) 168
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 2 cut(s) 444, 631
HspAI GCGC 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 2 cut(s) 810, 831
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 1 cut(s) 481
LpnPI CCDG 8 cut(s) 61, 299, 488, 515, 552, 559, 789, 832
Lsp1109I GCAGC 2 cut(s) 76, 562
LweI GCATC 1 cut(s) 496
MaeII ACGT 1 cut(s) 168
MalI GATC 2 cut(s) 812, 833
MboI GATC 2 cut(s) 810, 831
MboII GAAGA 2 cut(s) 385, 421
MfeI CAATTG 2 cut(s) 294, 521
MhlI GDGCHC 2 cut(s) 429, 486
MluCI AATT 4 cut(s) 185, 294, 493, 521
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 8 cut(s) 156, 166, 252, 325, 472, 477, 645, 694
Mph1103I ATGCAT 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 2 cut(s) 492, 584
MslI CAYNNNNRTG 1 cut(s) 632
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 503
MunI CAATTG 2 cut(s) 294, 521
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 2 cut(s) 810, 831
NlaIII CATG 2 cut(s) 444, 631
NlaIV GGNNCC 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 1 cut(s) 444
PaeI GCATGC 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 434
PctI GAATGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 3 cut(s) 178, 196, 767
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 3 cut(s) 66, 552, 807
Ppu21I YACGTR 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 576
PspPPI RGGWCCY 1 cut(s) 576
RsaI GTAC 2 cut(s) 167, 206
RsaNI GTAC 2 cut(s) 166, 205
RseI CAYNNNNRTG 1 cut(s) 632
SacI GAGCTC 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 492, 584
SatI GCNGC 3 cut(s) 65, 551, 806
Sau3AI GATC 2 cut(s) 810, 831
Sau96I GGNCC 1 cut(s) 576
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 2 cut(s) 429, 486
SfaNI GCATC 1 cut(s) 496
SinI GGWCC 1 cut(s) 576
SmiMI CAYNNNNRTG 1 cut(s) 632
SphI GCATGC 1 cut(s) 444
Sse9I AATT 4 cut(s) 185, 294, 493, 521
SsiI CCGC 2 cut(s) 160, 805
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 5 cut(s) 103, 340, 531, 558, 791
TaiI ACGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 16, 72, 765
TasI AATT 4 cut(s) 185, 294, 493, 521
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 3 cut(s) 178, 196, 767
Tru1I TTAA 2 cut(s) 492, 584
Tru9I TTAA 2 cut(s) 492, 584
TscAI CASTG 1 cut(s) 106
TseI GCWGC 2 cut(s) 64, 550
TspDTI ATGAA 7 cut(s) 11, 392, 417, 442, 555, 585, 654
TspGWI ACGGA 2 cut(s) 595, 679
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 1 cut(s) 576
XapI RAATTY 1 cut(s) 185
XceI RCATGY 1 cut(s) 444
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XmnI GAANNNNTTC 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.