MD08G1125300.v1.1

Ribonuclease H2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007200
Physical Location & Seq
Forward (+)
11784947 .. 11788299
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1125300.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCAGCAGATACTACTGAATCCGGAAACGACGTCGGACAATTGATTTCACTCCGCCATCCCAAGAACTCAATTGGATTAAGCAATCCTACACTTCTTGGTTTCTGGGTGAATATGTTACTGAAGAAGGGGGACGATCCTGGGAAGTTCAGGCTTTTAGATGACATAATCTTCATCGAAGGCTATCCGGGATACCATAAATTACTGTCCATTGCAGAGAACTGTATGCAATTAGTTTGCGAAATAAAAGAAGTCGGATCCTTCAAATTTTTTAGGCTGGATGATTCCAAGGTTCTGGCTTGGTTGTACCACAAGGTCTTGACTGATGCAGTTTCAGTATTGGGGGAATACGTGAAGGATGAGCCCTGGTTGAAGCCTTTGAGTGACCGTTTAAGGATAGATTTTACAGAAGCAAGGAGAAAAACATCAGATATCGAATTTCTCCCGACTGCACAGGAAGTAACCTGGGGCCTTGTAATGGTTCACAGGAAAAGGGTAAGAGTGAGATTAAGCCTACTCAAGCCGGAAGGCAAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2-Ydr279

Protein Analysis

180

Amino Acids

20.5

Weight (kDa)

8.83

Isoelectric Point (pI)

28.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2-Ydr279 PF09468 42 - 106 2.8e-07 Ydr279p protein family (RNase H2 complex component) wHTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 23
AciI CCGC 1 cut(s) 55
AclWI GGATC 3 cut(s) 131, 252, 265
AcsI RAATTY 2 cut(s) 266, 437
AcuI CTGAAG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 478
AgsI TTSAA 2 cut(s) 265, 373
AjnI CCWGG 3 cut(s) 139, 365, 464
AlwI GGATC 3 cut(s) 131, 252, 265
Aor13HI TCCGGA 1 cut(s) 23
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 266, 437
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 121
BamHI GGATCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 66
BciT130I CCWGG 3 cut(s) 141, 367, 466
BciVI GTATCC 1 cut(s) 185
BcnI CCSGG 1 cut(s) 189
BfuI GTATCC 1 cut(s) 185
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 4 cut(s) 141, 189, 367, 466
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 2 cut(s) 259, 470
BmrFI CCNGG 4 cut(s) 141, 189, 367, 466
BmsI GCATC 1 cut(s) 316
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 189
BsaAI YACGTR 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 4 cut(s) 140, 288, 365, 465
BsaWI WCCGGW 1 cut(s) 23
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 210
BseAI TCCGGA 1 cut(s) 23
BseBI CCWGG 3 cut(s) 141, 367, 466
BseDI CCNNGG 4 cut(s) 140, 288, 365, 465
BseGI GGATG 3 cut(s) 58, 286, 364
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMI GCAATG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 1 cut(s) 471
BsiSI CCGG 3 cut(s) 24, 188, 524
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 23
Bsp143I GATC 2 cut(s) 136, 257
BspACI CCGC 1 cut(s) 55
BspANI GGCC 1 cut(s) 471
BspEI TCCGGA 1 cut(s) 23
BspLI GGNNCC 2 cut(s) 259, 470
BspPI GGATC 3 cut(s) 131, 252, 265
BsrDI GCAATG 1 cut(s) 210
BssECI CCNNGG 4 cut(s) 140, 288, 365, 465
BssMI GATC 2 cut(s) 136, 257
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 3 cut(s) 141, 367, 466
Bst4CI ACNGT 3 cut(s) 207, 224, 389
BstACI GRCGYC 1 cut(s) 33
BstBAI YACGTR 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 533
BstF5I GGATG 3 cut(s) 58, 286, 364
BstKTI GATC 2 cut(s) 139, 260
BstMBI GATC 2 cut(s) 136, 257
BstNI CCWGG 3 cut(s) 141, 367, 466
BstSCI CCNGG 4 cut(s) 139, 187, 365, 464
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 257
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 257
BsuI GTATCC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 471
BtsCI GGATG 3 cut(s) 58, 286, 364
Cac8I GCNNGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 469
Csp6I GTAC 1 cut(s) 307
CviJI RGCY 9 cut(s) 154, 183, 277, 299, 364, 376, 471, 513, 523
CviKI_1 RGCY 9 cut(s) 154, 183, 277, 299, 364, 376, 471, 513, 523
CviQI GTAC 1 cut(s) 307
DpnI GATC 2 cut(s) 138, 259
DpnII GATC 2 cut(s) 136, 257
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 3 cut(s) 139, 365, 464
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 288
FaiI YATR 4 cut(s) 116, 167, 198, 227
FaqI GGGAC 1 cut(s) 146
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 3 cut(s) 45, 293, 371
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 1 cut(s) 471
HapII CCGG 3 cut(s) 24, 188, 524
Hin1I GRCGYC 1 cut(s) 33
HinfI GANTC 2 cut(s) 20, 284
HpaII CCGG 3 cut(s) 24, 188, 524
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 38, 257, 430
Hpy188III TCNNGA 3 cut(s) 24, 319, 445
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 5 cut(s) 121, 173, 271, 349, 521
HpyCH4III ACNGT 3 cut(s) 207, 224, 389
HpyCH4IV ACGT 2 cut(s) 33, 351
HpyCH4V TGCA 4 cut(s) 215, 229, 329, 452
HpySE526I ACGT 2 cut(s) 33, 351
Hsp92I GRCGYC 1 cut(s) 33
Kpn2I TCCGGA 1 cut(s) 23
Kzo9I GATC 2 cut(s) 136, 257
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 316
MaeII ACGT 2 cut(s) 33, 351
MaeIII GTNAC 3 cut(s) 117, 383, 460
MalI GATC 2 cut(s) 138, 259
MboI GATC 2 cut(s) 136, 257
MboII GAAGA 2 cut(s) 136, 163
MfeI CAATTG 2 cut(s) 41, 72
MflI RGATCY 1 cut(s) 257
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 6 cut(s) 41, 72, 200, 230, 266, 437
MmeI TCCRAC 2 cut(s) 16, 235
MroI TCCGGA 1 cut(s) 23
MseI TTAA 3 cut(s) 80, 392, 509
MspI CCGG 3 cut(s) 24, 188, 524
MspR9I CCNGG 4 cut(s) 141, 189, 367, 466
MunI CAATTG 2 cut(s) 41, 72
MvaI CCWGG 3 cut(s) 141, 367, 466
NciI CCSGG 1 cut(s) 189
NdeII GATC 2 cut(s) 136, 257
NlaIV GGNNCC 2 cut(s) 259, 470
NmuCI GTSAC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 20, 284
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 352
Psp6I CCWGG 3 cut(s) 139, 365, 464
PspGI CCWGG 3 cut(s) 139, 365, 464
PspN4I GGNNCC 2 cut(s) 259, 470
PspPI GGNCC 1 cut(s) 469
PsuI RGATCY 1 cut(s) 257
RsaI GTAC 1 cut(s) 308
RsaNI GTAC 1 cut(s) 307
SaqAI TTAA 3 cut(s) 80, 392, 509
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 136, 257
Sau96I GGNCC 1 cut(s) 469
ScrFI CCNGG 4 cut(s) 141, 189, 367, 466
SduI GDGCHC 1 cut(s) 366
SetI ASST 5 cut(s) 36, 294, 318, 354, 467
SfaNI GCATC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 6 cut(s) 41, 72, 200, 230, 266, 437
SsiI CCGC 1 cut(s) 55
StyD4I CCNGG 4 cut(s) 139, 187, 365, 464
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 207, 224, 389
TaiI ACGT 2 cut(s) 36, 354
TaqI TCGA 2 cut(s) 177, 435
TasI AATT 6 cut(s) 41, 72, 200, 230, 266, 437
TfiI GAWTC 2 cut(s) 20, 284
Tru1I TTAA 3 cut(s) 80, 392, 509
Tru9I TTAA 3 cut(s) 80, 392, 509
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 163
XapI RAATTY 2 cut(s) 266, 437
ZraI GACGTC 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.