pycom15g09540

Ribonuclease H2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007200
Physical Location & Seq
Forward (+)
6224883 .. 6227570
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09540.8

Sequence Viewer

Length: 843 bp
ATGGCTTGGTGGGAAGGCTCCGACGAAACCCGTCTTCTCATCGCAGCAGATACTGCTGAAACCGGAGACGACGCCGGACAATTGCTTTCGCTCCGCCATCCCAAGTCCGGAAACAAAATGTGCTGCCTTTTTGTAAATGAGGTGCTTCAGGAACTTAATTGGTTCAAGCAATCCTACACTTCTTGGTTTCTGGGTGATTACGTTGCTGAAGATGGTGGGTTGTATACTGCCACTCCAGTTGACCCTGTGTTCATTTTCTTGCCCATTTTCGAAGAAGCTAGAATGAAGAAGGGGGATGATCCTGGGAAGTTCAGGCTTTTAGATGATATAATCTTTATCGAAGGCTATCCTGGATACCATAAACTACTGTCCATTGCGGAGAACTGTATGCAATTAGTTTGTGAAATTAAAGAAATTGGATCCTCCAAATTTTTTAGGCTGGATGATTCCAAGGTTCTGGCTTGGTTGTACCACAAGGTATGTCAGCTAAAACAGAGACTATCCACTTTGGACCATAATTATGCTGCACGGGAAGAGAAAGAAATATTGACTGATGCAGTTTCAGTATTAGGGGAATACGTGAAGGATGAGCCCTGGTTGAAGCTTTTGAGTGACCGTTTGAGGATAGATTTTACAGAAGCAACAAGAAAAACGTCAGATATCGAATTTCTCCCAACTGCCACAGGAAGTAACCTGGGGCCTTTTAATGGTTTACAGGAAAAGAGTAAGAGTGAGAATAAGCCTACCCGATCTGGAAGGCAAGCGAAGAAGGCCAAAGCGGAGACGGAATCACATAACATCAGAGATATGTTTACTAGGGCTTCTTCAAGAAGGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2-Ydr279

Protein Analysis

281

Amino Acids

31.98

Weight (kDa)

6.04

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 224
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 3 cut(s) 94, 377, 779
AclWI GGATC 3 cut(s) 293, 414, 427
AcsI RAATTY 2 cut(s) 428, 665
AcuI CTGAAG 2 cut(s) 131, 228
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 107, 707
AgsI TTSAA 3 cut(s) 166, 601, 828
AjnI CCWGG 4 cut(s) 301, 349, 593, 693
AluBI AGCT 3 cut(s) 278, 487, 604
AluI AGCT 3 cut(s) 278, 487, 604
Alw26I GTCTC 3 cut(s) 60, 490, 776
AlwI GGATC 3 cut(s) 293, 414, 427
AlwNI CAGNNNCTG 1 cut(s) 53
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 2 cut(s) 698, 771
ApeKI GCWGC 3 cut(s) 44, 123, 524
ApoI RAATTY 2 cut(s) 428, 665
AspS9I GGNCC 2 cut(s) 511, 698
AsuHPI GGTGA 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 270
AvaII GGWCC 1 cut(s) 511
BamHI GGATCC 1 cut(s) 419
BanII GRGCYC 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 56, 110, 511
BccI CCATC 2 cut(s) 105, 206
BciT130I CCWGG 4 cut(s) 303, 351, 595, 695
BciVI GTATCC 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 60, 490, 776
BfaI CTAG 2 cut(s) 279, 816
BfuI GTATCC 1 cut(s) 347
BisI GCNGC 3 cut(s) 45, 124, 525
BlsI GCNGC 3 cut(s) 46, 125, 526
Bme1390I CCNGG 4 cut(s) 303, 351, 595, 695
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 2 cut(s) 511, 698
BmiI GGNNCC 3 cut(s) 19, 421, 699
BmrFI CCNGG 4 cut(s) 303, 351, 595, 695
BmsI GCATC 1 cut(s) 544
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 302, 450, 593, 694
BsaWI WCCGGW 2 cut(s) 62, 107
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 707
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 4 cut(s) 303, 351, 595, 695
BseDI CCNNGG 4 cut(s) 302, 450, 593, 694
BseGI GGATG 4 cut(s) 97, 301, 448, 592
BseLI CCNNNNNNNGG 2 cut(s) 107, 707
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 56, 110, 511
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 2 cut(s) 700, 773
BsiSI CCGG 3 cut(s) 63, 75, 108
BslI CCNNNNNNNGG 2 cut(s) 107, 707
BsmAI GTCTC 3 cut(s) 60, 490, 776
BsmBI CGTCTC 2 cut(s) 60, 776
BsnI GGCC 2 cut(s) 700, 773
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 594
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 298, 419, 749
BspACI CCGC 3 cut(s) 94, 377, 779
BspANI GGCC 2 cut(s) 700, 773
BspEI TCCGGA 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 19, 421, 699
BspPI GGATC 3 cut(s) 293, 414, 427
BspT104I TTCGAA 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 4 cut(s) 302, 450, 593, 694
BssMI GATC 3 cut(s) 298, 419, 749
BssNAI GTATAC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 450
Bst1107I GTATAC 1 cut(s) 225
Bst2UI CCWGG 4 cut(s) 303, 351, 595, 695
Bst4CI ACNGT 3 cut(s) 369, 386, 617
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 762
BstF5I GGATG 4 cut(s) 97, 301, 448, 592
BstKTI GATC 3 cut(s) 301, 422, 752
BstMAI GTCTC 3 cut(s) 60, 490, 776
BstMBI GATC 3 cut(s) 298, 419, 749
BstMWI GCNNNNNNNGC 2 cut(s) 53, 770
BstNI CCWGG 4 cut(s) 303, 351, 595, 695
BstSCI CCNGG 4 cut(s) 301, 349, 593, 693
BstV1I GCAGC 3 cut(s) 56, 110, 511
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 419
BstXI CCANNNNNNTGG 1 cut(s) 457
BstYI RGATCY 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 225
BsuI GTATCC 1 cut(s) 347
BsuRI GGCC 2 cut(s) 700, 773
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 4 cut(s) 97, 301, 448, 592
Cac8I GCNNGC 1 cut(s) 762
CaiI CAGNNNCTG 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 511, 698
CseI GACGC 1 cut(s) 80
Csp6I GTAC 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviQI GTAC 1 cut(s) 469
DpnI GATC 3 cut(s) 300, 421, 751
DpnII GATC 3 cut(s) 298, 419, 749
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 511
Eco57I CTGAAG 2 cut(s) 131, 228
EcoO109I RGGNCCY 1 cut(s) 698
EcoRII CCWGG 4 cut(s) 301, 349, 593, 693
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 450
Esp3I CGTCTC 2 cut(s) 60, 776
FaiI YATR 9 cut(s) 225, 329, 360, 389, 481, 516, 522, 795, 809
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 3 cut(s) 45, 124, 525
FokI GGATG 4 cut(s) 84, 308, 455, 599
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 3 cut(s) 45, 124, 525
FspBI CTAG 2 cut(s) 279, 816
GluI GCNGC 3 cut(s) 45, 124, 525
GsuI CTGGAG 1 cut(s) 219
HaeIII GGCC 2 cut(s) 700, 773
HapII CCGG 3 cut(s) 63, 75, 108
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 2 cut(s) 446, 788
HpaII CCGG 3 cut(s) 63, 75, 108
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 4 cut(s) 225, 241, 713, 813
Hpy188I TCNGA 3 cut(s) 22, 658, 803
Hpy188III TCNNGA 4 cut(s) 108, 149, 753, 828
Hpy8I GTNNAC 4 cut(s) 225, 241, 713, 813
Hpy99I CGWCG 2 cut(s) 26, 74
HpyAV CCTTC 7 cut(s) 8, 283, 335, 577, 750, 763, 825
HpyCH4III ACNGT 3 cut(s) 369, 386, 617
HpyCH4IV ACGT 3 cut(s) 201, 579, 653
HpyCH4V TGCA 3 cut(s) 391, 527, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 770
HpySE526I ACGT 3 cut(s) 201, 579, 653
Hsp92I GRCGYC 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 3 cut(s) 298, 419, 749
LmnI GCTCC 2 cut(s) 23, 96
Lsp1109I GCAGC 3 cut(s) 56, 110, 511
LweI GCATC 1 cut(s) 544
MaeI CTAG 2 cut(s) 279, 816
MaeII ACGT 3 cut(s) 201, 579, 653
MaeIII GTNAC 2 cut(s) 611, 689
MalI GATC 3 cut(s) 300, 421, 751
MboI GATC 3 cut(s) 298, 419, 749
MboII GAAGA 7 cut(s) 26, 221, 284, 298, 545, 778, 816
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 8 cut(s) 80, 157, 392, 405, 414, 428, 517, 665
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 3 cut(s) 133, 433, 615
MroI TCCGGA 1 cut(s) 107
MseI TTAA 3 cut(s) 156, 408, 705
MslI CAYNNNNRTG 1 cut(s) 519
MspI CCGG 3 cut(s) 63, 75, 108
MspR9I CCNGG 4 cut(s) 303, 351, 595, 695
MunI CAATTG 1 cut(s) 80
MvaI CCWGG 4 cut(s) 303, 351, 595, 695
MwoI GCNNNNNNNGC 2 cut(s) 53, 770
NdeII GATC 3 cut(s) 298, 419, 749
NlaIV GGNNCC 3 cut(s) 19, 421, 699
NmuCI GTSAC 1 cut(s) 611
NspV TTCGAA 1 cut(s) 270
PfeI GAWTC 2 cut(s) 446, 788
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 3 cut(s) 46, 125, 526
Ppu21I YACGTR 1 cut(s) 580
Psp6I CCWGG 4 cut(s) 301, 349, 593, 693
PspGI CCWGG 4 cut(s) 301, 349, 593, 693
PspN4I GGNNCC 3 cut(s) 19, 421, 699
PspPI GGNCC 2 cut(s) 511, 698
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 419
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 1 cut(s) 519
SaqAI TTAA 3 cut(s) 156, 408, 705
SatI GCNGC 3 cut(s) 45, 124, 525
Sau3AI GATC 3 cut(s) 298, 419, 749
Sau96I GGNCC 2 cut(s) 511, 698
ScrFI CCNGG 4 cut(s) 303, 351, 595, 695
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 1 cut(s) 544
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 519
Sse9I AATT 8 cut(s) 80, 157, 392, 405, 414, 428, 517, 665
SsiI CCGC 3 cut(s) 94, 377, 779
SspI AATATT 1 cut(s) 546
SspMI CTAG 2 cut(s) 279, 816
StyD4I CCNGG 4 cut(s) 301, 349, 593, 693
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 3 cut(s) 369, 386, 617
TaiI ACGT 3 cut(s) 204, 582, 656
TaqI TCGA 3 cut(s) 270, 339, 663
TasI AATT 8 cut(s) 80, 157, 392, 405, 414, 428, 517, 665
TfiI GAWTC 2 cut(s) 446, 788
Tru1I TTAA 3 cut(s) 156, 408, 705
Tru9I TTAA 3 cut(s) 156, 408, 705
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 3 cut(s) 44, 123, 524
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 2 cut(s) 241, 299
TspGWI ACGGA 1 cut(s) 800
VpaK11BI GGWCC 1 cut(s) 511
XapI RAATTY 2 cut(s) 428, 665
XmiI GTMKAC 1 cut(s) 224
XspI CTAG 2 cut(s) 279, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.