Rroxscaffold_7G00162910

Ribonuclease H2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007200
Physical Location & Seq
Forward (+)
5270818 .. 5273232
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162910.1

Sequence Viewer

Length: 846 bp
ATGGCTTGGTGGCAAGGTTTCGATGAAACTCGTCTTCTCATAGCAGCTGATCCTCCTGTCGCCGGAGACGGCGCCGGACAACTGATTTCGCTCCGCCACCCAAAATCCGGAAACACAATGTGCTGTGTTTTAGTTGATGGGATGCTGCAGGAACTTAATTGGTTCAGGCAGTCCTACAGTTCTTGGTTTCTTGGAGACTATGTTTCTGAGGATGGTGGGATATATACCGCCACTCGTGTTGATCCTGTTTTCATTTTGTTGCCCATTTTTGAGGAAGCTAGAATGAAGAAAGGTGACGATCCTGGCAAGTACAGGCCATTGGATGAGATAATCTTCGTTGATGGCTATCCTGGATATCAGCAACTTTTGTCCATTGCGGAGATTTCTATGCAAGTAGTTTGTGATATCAAAGAAATTGATTCCAACAAATATTTTAGGCTTGACGACTCCAAGGTTCTGGCTTGGTTGTACCACAAGGCATTCCAGCTAAAACAGACGCTATCCACGTTGGACAGTAATTATGCTGCAAGGGAAGAAAAGCATAGATTGGCTGATGCGGTTTCAATATTAGGGGAATACCTGAAGGGTGAGCCCTGGTTGAAGCTTTTGTGTGACTGTTTGAGGATAGATTTACCAGAGGCACCAAAAAAAGCACCAGATGCTGAAACTCTCCCAACTGCCAACGAAAGTAGCCCAGGGATTTGGAATGGTTCACAGGAAAAGAGCAAGAGTGACAATAAGGTTACTACCAGAGCTGCTGGAAGGCAAGCTAAAAAGATCAAACTGGAGACGGGATCACATAACATCAGAGACATGTTCACTAGGGCCTCATCAAGTAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2-Ydr279

Protein Analysis

281

Amino Acids

31.6

Weight (kDa)

5.9

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ydr279_N PF17745 27 - 81 6.7e-06 Ydr279p protein triple barrel domain
RNase_H2-Ydr279 PF09468 84 - 167 1.9e-10 Ydr279p protein family (RNase H2 complex component) wHTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 640
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 4 cut(s) 94, 228, 377, 557
AclWI GGATC 4 cut(s) 44, 236, 293, 802
AcuI CTGAAG 1 cut(s) 602
AcyI GRCGYC 1 cut(s) 72
AdeI CACNNNGTG 1 cut(s) 120
AfaI GTAC 2 cut(s) 311, 470
AfiI CCNNNNNNNGG 2 cut(s) 62, 107
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 2 cut(s) 564, 601
AjnI CCWGG 4 cut(s) 301, 349, 593, 694
AluBI AGCT 6 cut(s) 47, 278, 487, 604, 755, 770
AluI AGCT 6 cut(s) 47, 278, 487, 604, 755, 770
Alw26I GTCTC 4 cut(s) 60, 189, 782, 804
AlwI GGATC 4 cut(s) 44, 236, 293, 802
AlwNI CAGNNNCTG 1 cut(s) 662
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 2 cut(s) 314, 825
ApeKI GCWGC 4 cut(s) 44, 145, 524, 755
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 825
AsuHPI GGTGA 2 cut(s) 305, 599
BanI GGYRCC 2 cut(s) 71, 640
BanII GRGCYC 1 cut(s) 594
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 4 cut(s) 56, 132, 511, 742
BccI CCATC 3 cut(s) 131, 206, 335
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 4 cut(s) 303, 351, 595, 696
BcoDI GTCTC 4 cut(s) 60, 189, 782, 804
BfaI CTAG 2 cut(s) 279, 822
BfmI CTRYAG 2 cut(s) 146, 175
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 4 cut(s) 45, 146, 525, 756
BlsI GCNGC 4 cut(s) 46, 147, 526, 757
Bme1390I CCNGG 4 cut(s) 303, 351, 595, 696
BmgT120I GGNCC 1 cut(s) 825
BmiI GGNNCC 2 cut(s) 73, 642
BmrFI CCNGG 4 cut(s) 303, 351, 595, 696
BmsI GCATC 3 cut(s) 132, 544, 649
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 450, 593, 694, 695
BsaWI WCCGGW 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 107
Bse1I ACTGG 1 cut(s) 789
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 4 cut(s) 303, 351, 595, 696
BseDI CCNNGG 4 cut(s) 450, 593, 694, 695
BseGI GGATG 3 cut(s) 147, 217, 328
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 62, 107
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 789
BseXI GCAGC 4 cut(s) 56, 132, 511, 742
BshFI GGCC 2 cut(s) 316, 827
BshNI GGYRCC 2 cut(s) 71, 640
BsiSI CCGG 3 cut(s) 63, 75, 108
BslI CCNNNNNNNGG 2 cut(s) 62, 107
BsmAI GTCTC 4 cut(s) 60, 189, 782, 804
BsmBI CGTCTC 2 cut(s) 60, 782
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 2 cut(s) 316, 827
Bsp1286I GDGCHC 1 cut(s) 594
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 5 cut(s) 49, 241, 298, 777, 794
BspACI CCGC 4 cut(s) 94, 228, 377, 557
BspANI GGCC 2 cut(s) 316, 827
BspCNI CTCAG 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 73, 642
BspMAI CTGCAG 1 cut(s) 150
BspPI GGATC 4 cut(s) 44, 236, 293, 802
BspT107I GGYRCC 2 cut(s) 71, 640
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 789
BssECI CCNNGG 4 cut(s) 450, 593, 694, 695
BssMI GATC 5 cut(s) 49, 241, 298, 777, 794
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 234
Bst2UI CCWGG 4 cut(s) 303, 351, 595, 696
Bst4CI ACNGT 3 cut(s) 179, 515, 617
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 1 cut(s) 207
BstF5I GGATG 3 cut(s) 147, 217, 328
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 5 cut(s) 52, 244, 301, 780, 797
BstMAI GTCTC 4 cut(s) 60, 189, 782, 804
BstMBI GATC 5 cut(s) 49, 241, 298, 777, 794
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 4 cut(s) 303, 351, 595, 696
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 4 cut(s) 301, 349, 593, 694
BstSFI CTRYAG 2 cut(s) 146, 175
BstV1I GCAGC 4 cut(s) 56, 132, 511, 742
BstV2I GAAGAC 1 cut(s) 26
BstXI CCANNNNNNTGG 2 cut(s) 457, 702
BsuRI GGCC 2 cut(s) 316, 827
BtsCI GGATG 3 cut(s) 147, 217, 328
Cac8I GCNNGC 1 cut(s) 768
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 825
CseI GACGC 1 cut(s) 505
Csp6I GTAC 2 cut(s) 310, 469
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 1 cut(s) 814
CviQI GTAC 2 cut(s) 310, 469
DdeI CTNAG 1 cut(s) 207
DinI GGCGCC 1 cut(s) 73
DpnI GATC 5 cut(s) 51, 243, 300, 779, 796
DpnII GATC 5 cut(s) 49, 241, 298, 777, 794
DraIII CACNNNGTG 1 cut(s) 120
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 594
Eco32I GATATC 2 cut(s) 356, 406
Eco57I CTGAAG 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 825
EcoRII CCWGG 4 cut(s) 301, 349, 593, 694
EcoRV GATATC 2 cut(s) 356, 406
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 594
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 450
Esp3I CGTCTC 2 cut(s) 60, 782
FaeI CATG 1 cut(s) 817
FaiI YATR 9 cut(s) 41, 201, 223, 225, 389, 522, 543, 801, 815
FatI CATG 1 cut(s) 813
Fnu4HI GCNGC 4 cut(s) 45, 146, 525, 756
FokI GGATG 3 cut(s) 154, 224, 335
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 4 cut(s) 45, 146, 525, 756
FspBI CTAG 2 cut(s) 279, 822
GlaI GCGC 1 cut(s) 73
GluI GCNGC 4 cut(s) 45, 146, 525, 756
GsuI CTGGAG 1 cut(s) 806
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 316, 827
HapII CCGG 3 cut(s) 63, 75, 108
HgaI GACGC 1 cut(s) 505
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 817
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 2 cut(s) 419, 446
HpaII CCGG 3 cut(s) 63, 75, 108
HphI GGTGA 2 cut(s) 305, 599
Hpy166II GTNNAC 2 cut(s) 713, 819
Hpy188I TCNGA 2 cut(s) 208, 809
Hpy188III TCNNGA 1 cut(s) 108
Hpy8I GTNNAC 2 cut(s) 713, 819
HpyAV CCTTC 2 cut(s) 577, 756
HpyCH4III ACNGT 3 cut(s) 179, 515, 617
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 3 cut(s) 148, 391, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 1 cut(s) 207
HpySE526I ACGT 1 cut(s) 506
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 817
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 5 cut(s) 49, 241, 298, 777, 794
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 4 cut(s) 56, 132, 511, 742
LweI GCATC 3 cut(s) 132, 544, 649
MaeI CTAG 2 cut(s) 279, 822
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 4 cut(s) 293, 611, 731, 742
MalI GATC 5 cut(s) 51, 243, 300, 779, 796
MboI GATC 5 cut(s) 49, 241, 298, 777, 794
MboII GAAGA 4 cut(s) 26, 298, 325, 545
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 3 cut(s) 157, 414, 517
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 2 cut(s) 447, 489
MnlI CCTC 6 cut(s) 63, 202, 265, 615, 631, 838
MroI TCCGGA 1 cut(s) 107
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 3 cut(s) 63, 75, 108
MspR9I CCNGG 4 cut(s) 303, 351, 595, 696
Mva1269I GAATGC 1 cut(s) 479
MvaI CCWGG 4 cut(s) 303, 351, 595, 696
MwoI GCNNNNNNNGC 1 cut(s) 659
NarI GGCGCC 1 cut(s) 72
NdeII GATC 5 cut(s) 49, 241, 298, 777, 794
NlaIII CATG 1 cut(s) 817
NlaIV GGNNCC 2 cut(s) 73, 642
NmuCI GTSAC 3 cut(s) 293, 611, 731
NspI RCATGY 1 cut(s) 817
PasI CCCWGGG 1 cut(s) 695
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PctI GAATGC 1 cut(s) 479
PfeI GAWTC 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 4 cut(s) 46, 147, 526, 757
PleI GAGTC 1 cut(s) 440
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 440
PscI ACATGT 1 cut(s) 813
Psp6I CCWGG 4 cut(s) 301, 349, 593, 694
PspGI CCWGG 4 cut(s) 301, 349, 593, 694
PspN4I GGNNCC 2 cut(s) 73, 642
PspPI GGNCC 1 cut(s) 825
PstI CTGCAG 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 662
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 311, 470
RsaNI GTAC 2 cut(s) 310, 469
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 4 cut(s) 45, 146, 525, 756
Sau3AI GATC 5 cut(s) 49, 241, 298, 777, 794
Sau96I GGNCC 1 cut(s) 825
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 4 cut(s) 303, 351, 595, 696
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 3 cut(s) 132, 544, 649
SfcI CTRYAG 2 cut(s) 146, 175
SfoI GGCGCC 1 cut(s) 73
Sse9I AATT 3 cut(s) 157, 414, 517
SsiI CCGC 4 cut(s) 94, 228, 377, 557
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 2 cut(s) 431, 567
SspMI CTAG 2 cut(s) 279, 822
StyD4I CCNGG 4 cut(s) 301, 349, 593, 694
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 3 cut(s) 179, 515, 617
TaiI ACGT 1 cut(s) 509
TaqI TCGA 1 cut(s) 21
TasI AATT 3 cut(s) 157, 414, 517
TatI WGTACW 1 cut(s) 309
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TseFI GTSAC 3 cut(s) 293, 611, 731
TseI GCWGC 4 cut(s) 44, 145, 524, 755
Tsp45I GTSAC 3 cut(s) 293, 611, 731
TspDTI ATGAA 3 cut(s) 39, 241, 299
XceI RCATGY 1 cut(s) 817
XspI CTAG 2 cut(s) 279, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.