Rorug06G0339500

Ribonuclease H2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005579
Physical Location & Seq
Forward (+)
49091538 .. 49093864
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0339500.1

Sequence Viewer

Length: 612 bp
ATGCCTCGGTATTACTGCGATTACTGCGATACGTATTTGACCCACGATTCGCCTTCGGTTCGAAAGCAGCACAATGCGGGTTACAAACACAAGGCAAATGTGCGGCAGTACTATCAGCAATTTGAGGAGCAACAAACTCAGAGTTTGATTGACCAAAGGATTAAGGAACATCTTGGCCAGACTGCAGCTTACGGGCAAGTTGGCGCAGCTTACAATCAGCATCTAATGGCTCGGCCTCGCCTTCCTGTTCTACCAAACCCTATGATGCCACAAATGCCTGGAGGTCCACCAATGGCTCCAGGGATTAGACTTCCTGTTTTGCCAAGACCTGGAGGTCCACCAATGGCTCCAGGAGTATACCAACAACAACCCATGATGCAAATGATGGCTCCACCTGGGGCTCCTGGGATGCCTGGTCAAGTAAATATTCCCATGAGGCCTCCCACTTTGAGTGCTCCACCATCAGTTCCCGGCAGTGCACCACCCAATGCTACTAACGGTGCCCCTTCTGTGGCTGCACCCCCAATGTATCAACCTAATCCAACAGCGCCAACAAGCGGAGGCTATGATAGTTTCAATTTAAATGCACAGCCTCCTGAATCTAGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2-Ydr279

Protein Analysis

203

Amino Acids

21.97

Weight (kDa)

9.12

Isoelectric Point (pI)

69.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-U1 PF06220 1 - 38 5.8e-22 U1 zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 500
AccB7I CCANNNNNTGG 1 cut(s) 329
AccI GTMKAC 1 cut(s) 357
AciI CCGC 3 cut(s) 77, 103, 558
AcoI YGGCCR 1 cut(s) 175
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 329, 511, 557
AgsI TTSAA 1 cut(s) 577
AjnI CCWGG 7 cut(s) 277, 298, 328, 349, 394, 403, 412
AluBI AGCT 2 cut(s) 188, 209
AluI AGCT 2 cut(s) 188, 209
Alw21I GWGCWC 2 cut(s) 457, 481
Alw44I GTGCAC 1 cut(s) 477
AoxI GGCC 3 cut(s) 175, 233, 437
ApaLI GTGCAC 1 cut(s) 477
ApeKI GCWGC 4 cut(s) 67, 185, 206, 515
ArsI GACNNNNNNTTYG 2 cut(s) 31, 63
AspLEI GCGC 2 cut(s) 206, 550
AspS9I GGNCC 2 cut(s) 284, 335
AsuC2I CCSGG 1 cut(s) 471
AsuII TTCGAA 1 cut(s) 61
AvaII GGWCC 2 cut(s) 284, 335
BaeGI GKGCMC 2 cut(s) 481, 505
BalI TGGCCA 1 cut(s) 177
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 403
Bbv12I GWGCWC 2 cut(s) 457, 481
BbvI GCAGC 4 cut(s) 79, 197, 218, 502
BccI CCATC 2 cut(s) 379, 469
BciT130I CCWGG 7 cut(s) 279, 300, 330, 351, 396, 405, 414
BcnI CCSGG 1 cut(s) 471
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 551
BisI GCNGC 5 cut(s) 68, 104, 186, 207, 516
BlsI GCNGC 5 cut(s) 69, 105, 187, 208, 517
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 8 cut(s) 279, 300, 330, 351, 396, 405, 414, 471
Bme18I GGWCC 2 cut(s) 284, 335
BmgT120I GGNCC 2 cut(s) 284, 335
BmiI GGNNCC 5 cut(s) 297, 348, 390, 402, 502
BmrFI CCNGG 8 cut(s) 279, 300, 330, 351, 396, 405, 414, 471
BmsI GCATC 4 cut(s) 229, 255, 366, 399
BpmI CTGGAG 4 cut(s) 282, 300, 333, 351
Bpu14I TTCGAA 1 cut(s) 61
BpuMI CCSGG 1 cut(s) 471
BsaAI YACGTR 1 cut(s) 33
BsaJI CCNNGG 4 cut(s) 5, 299, 395, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 511, 557
BseBI CCWGG 7 cut(s) 279, 300, 330, 351, 396, 405, 414
BseDI CCNNGG 4 cut(s) 5, 299, 395, 404
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 3 cut(s) 329, 511, 557
BseMII CTCAG 1 cut(s) 152
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 2 cut(s) 481, 505
BseXI GCAGC 4 cut(s) 79, 197, 218, 502
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 3 cut(s) 177, 235, 439
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 457, 481
BsiSI CCGG 1 cut(s) 471
BslI CCNNNNNNNGG 3 cut(s) 329, 511, 557
BsnI GGCC 3 cut(s) 177, 235, 439
Bsp119I TTCGAA 1 cut(s) 61
Bsp1286I GDGCHC 4 cut(s) 403, 457, 481, 505
BspACI CCGC 3 cut(s) 77, 103, 558
BspANI GGCC 3 cut(s) 177, 235, 439
BspCNI CTCAG 1 cut(s) 151
BspLI GGNNCC 5 cut(s) 297, 348, 390, 402, 502
BspMAI CTGCAG 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 61
BspT107I GGYRCC 1 cut(s) 500
BssECI CCNNGG 4 cut(s) 5, 299, 395, 404
BssNAI GTATAC 1 cut(s) 358
Bst1107I GTATAC 1 cut(s) 358
Bst2UI CCWGG 7 cut(s) 279, 300, 330, 351, 396, 405, 414
Bst4CI ACNGT 1 cut(s) 500
BstBAI YACGTR 1 cut(s) 33
BstBI TTCGAA 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 551
BstHHI GCGC 2 cut(s) 206, 550
BstMWI GCNNNNNNNGC 2 cut(s) 24, 274
BstNI CCWGG 7 cut(s) 279, 300, 330, 351, 396, 405, 414
BstSCI CCNGG 8 cut(s) 277, 298, 328, 349, 394, 403, 412, 469
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 2 cut(s) 481, 505
BstSNI TACGTA 1 cut(s) 33
BstV1I GCAGC 4 cut(s) 79, 197, 218, 502
BstZ17I GTATAC 1 cut(s) 358
BsuRI GGCC 3 cut(s) 177, 235, 439
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 481
BtsIMutI CAGTG 1 cut(s) 481
CfoI GCGC 2 cut(s) 206, 550
Cfr13I GGNCC 2 cut(s) 284, 335
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 373, 433
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 1 cut(s) 138
DraI TTTAAA 1 cut(s) 582
DrdI GACNNNNNNGTC 1 cut(s) 333
DseDI GACNNNNNNGTC 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 175
Eco105I TACGTA 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 403
Eco47I GGWCC 2 cut(s) 284, 335
EcoRII CCWGG 7 cut(s) 277, 298, 328, 349, 394, 403, 412
EcoT38I GRGCYC 1 cut(s) 403
FaeI CATG 2 cut(s) 376, 436
FaiI YATR 5 cut(s) 263, 358, 374, 434, 567
FatI CATG 2 cut(s) 372, 432
FauI CCCGC 1 cut(s) 70
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 68, 104, 186, 207, 516
FokI GGATG 1 cut(s) 421
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 5 cut(s) 68, 104, 186, 207, 516
FspBI CTAG 1 cut(s) 603
GlaI GCGC 2 cut(s) 205, 549
GluI GCNGC 5 cut(s) 68, 104, 186, 207, 516
GsuI CTGGAG 4 cut(s) 282, 300, 333, 351
HaeII RGCGCY 1 cut(s) 551
HaeIII GGCC 3 cut(s) 177, 235, 439
HapII CCGG 1 cut(s) 471
HhaI GCGC 2 cut(s) 206, 550
Hin1II CATG 2 cut(s) 376, 436
Hin6I GCGC 2 cut(s) 204, 548
HinP1I GCGC 2 cut(s) 204, 548
HinfI GANTC 2 cut(s) 47, 599
HpaII CCGG 1 cut(s) 471
Hpy166II GTNNAC 4 cut(s) 287, 338, 358, 479
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 1 cut(s) 596
Hpy8I GTNNAC 4 cut(s) 287, 338, 358, 479
HpyAV CCTTC 3 cut(s) 63, 251, 516
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 185, 379, 479, 518, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 274
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 2 cut(s) 376, 436
HspAI GCGC 2 cut(s) 204, 548
LmnI GCTCC 6 cut(s) 127, 301, 352, 394, 406, 460
Lsp1109I GCAGC 4 cut(s) 79, 197, 218, 502
LweI GCATC 4 cut(s) 229, 255, 366, 399
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 1 cut(s) 80
MhlI GDGCHC 4 cut(s) 403, 457, 481, 505
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 2 cut(s) 119, 577
MluNI TGGCCA 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 9 cut(s) 15, 118, 246, 275, 326, 429, 450, 554, 603
Mox20I TGGCCA 1 cut(s) 177
MscI TGGCCA 1 cut(s) 177
MseI TTAA 2 cut(s) 162, 581
Msp20I TGGCCA 1 cut(s) 177
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 8 cut(s) 279, 300, 330, 351, 396, 405, 414, 471
MvaI CCWGG 7 cut(s) 279, 300, 330, 351, 396, 405, 414
MwoI GCNNNNNNNGC 2 cut(s) 24, 274
NciI CCSGG 1 cut(s) 471
NlaIII CATG 2 cut(s) 376, 436
NlaIV GGNNCC 5 cut(s) 297, 348, 390, 402, 502
NmeAIII GCCGAG 1 cut(s) 211
NspV TTCGAA 1 cut(s) 61
PceI AGGCCT 1 cut(s) 439
PfeI GAWTC 2 cut(s) 47, 599
PflMI CCANNNNNTGG 1 cut(s) 329
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 5 cut(s) 69, 105, 187, 208, 517
Ppu21I YACGTR 1 cut(s) 33
Psp6I CCWGG 7 cut(s) 277, 298, 328, 349, 394, 403, 412
PspGI CCWGG 7 cut(s) 277, 298, 328, 349, 394, 403, 412
PspN4I GGNNCC 5 cut(s) 297, 348, 390, 402, 502
PspPI GGNCC 2 cut(s) 284, 335
PstI CTGCAG 1 cut(s) 187
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 162, 581
SatI GCNGC 5 cut(s) 68, 104, 186, 207, 516
Sau96I GGNCC 2 cut(s) 284, 335
ScaI AGTACT 1 cut(s) 110
ScrFI CCNGG 8 cut(s) 279, 300, 330, 351, 396, 405, 414, 471
SduI GDGCHC 4 cut(s) 403, 457, 481, 505
SetI ASST 8 cut(s) 35, 190, 211, 286, 331, 337, 397, 538
SfaNI GCATC 4 cut(s) 229, 255, 366, 399
SfcI CTRYAG 1 cut(s) 183
SfuI TTCGAA 1 cut(s) 61
SinI GGWCC 2 cut(s) 284, 335
SmiI ATTTAAAT 1 cut(s) 582
SnaBI TACGTA 1 cut(s) 33
Sse9I AATT 2 cut(s) 119, 577
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 3 cut(s) 77, 103, 558
SspI AATATT 1 cut(s) 427
SspMI CTAG 1 cut(s) 603
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 8 cut(s) 277, 298, 328, 349, 394, 403, 412, 469
SwaI ATTTAAAT 1 cut(s) 582
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 1 cut(s) 35
TaqI TCGA 1 cut(s) 61
TasI AATT 2 cut(s) 119, 577
TatI WGTACW 1 cut(s) 108
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 47, 599
Tru1I TTAA 2 cut(s) 162, 581
Tru9I TTAA 2 cut(s) 162, 581
TscAI CASTG 1 cut(s) 481
TseI GCWGC 4 cut(s) 67, 185, 206, 515
TspRI CASTG 1 cut(s) 481
Van91I CCANNNNNTGG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 477
VpaK11BI GGWCC 2 cut(s) 284, 335
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.