MD08G1125400.v1.1

Thioredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005579
Physical Location & Seq
Forward (+)
11789802 .. 11797065
7264 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1125400.v1.1.491

Sequence Viewer

Length: 3543 bp
ATGGCAGTGCATAGCAGCAATATAGCAACCGTTCTCGAATCTGAAGGAGTTGAGTTTCTTCTATCCGGTGACACAAAGGTACCGTTATCATCATGTAATGGGAAGACAGTGTGCCTCTACTTCTCTGCAAATTGGTGTCGGCCTTGCAAGGCTCTTACCCCTCAGCTGGTGAGACTTTATGAAGTGCTTAGATTGGAAGGAAAAGAGTTTGAGATAATCTTCATCTCCTTCGACCCCAATGAGGAAAAGTTCAATGAACACTTCAAGTGCATGCCATGGCTGGCAGTGCCCTTTGATGCCAATCTTCATAAAAGATTGAGTGACATGTACCGCATAGATCGCATCCCATCACTCATTTCATTAAGTTCAGATGGATTATCCATTGAGGAAGACTTGGTTGGATTAATTGAAGACTATGGAGCTGATGCATTTCCATTCACTAACAAGAGAAGAGTAGAGCTAAAGGCCGCCGATGAAGCAAAGCGTGAAGGGGGAAAAATCGAAGAGTTGTTGACGCATCAAGGACGAAACCATGTCTTGGCTAGTGATGGCAGAGAGATACAGGTGTCTGAACTTGTTGGTAAGACAGTAGGCCTTTACTTTGGTGCACACTGGAGCCCTCCTTGCCGTGCTTTTACTTCCAAACTCATAGAAGCATACGAAGAGCTCATGACATCCGGTGATCAAGACTTCGAAATCATCCTAGTCTCCACAGACCGAGACCTCAAAGAGTTTGAACTCAACACTAGTAGCATGCCATGGCTTGCCATACCCTACCAGGACAAAACAAGACAAGACCTTTGTAGAATCTTCGACATCAAAGTGATTCCGGCTCTGGTTCTGATAGGACCGAATGGGAAGGCGATTAGCACAAATGGGAAGGCCATGGTCTCCTTACACGGTGCCAAGGCCTTCCCGTTCACAGAAGCGAGGATCACAGAGCTCGAAGCAGAATTGAGAAAGGAAGGAGAGGCATTGCCTCCTCAAGTGAAGGACTTGAAGCATAAACATTTGCTTAAGTTAGAGATGGCCAAAGCATATGTGTGTGATTATTGCCAAAAACAGGGGAGGTTTTGGGCATTTTCTTGTGGTGTTTGTGATTACGATCTTCATCCAAACTGCGTAGAAAAATCGTTGGTGGAAAAGAGTGAGAGAGACCGTGGGAGTGGAGAGGGAGGGAGAGAGGAAAAGAAGAGAGAGAGAGAGAGAGAAATGTGGCTACCCAATAATCAAGTGGACAAGAAGGAAGCAGGGAATGGGATTGTGGCAGTGGCAATAGACAGGGACAAGAACAGCCAATGTGCTCTCAAATGGGCAATTGACAGTCTCATCAAACCAGGCCAATCTGTTCTTCTCATCCATGTCAAGGTCAAGCATTCCATAATTTCTCAGTCGCATTCTCTTTCCAATTCCACCCCAAGAAGTAATCCAAATTGGGACCAACAGTTGGTATGCAAAGACCCTGATCCCCAAACCCGGGAATTGTTCCTTCCCTTCCGTGTTTTTTGCACACGTAAAAATATACAATGCAGAGACCTTGTACTAGAAGACTCCGATGTAACGAAAGCATTAGTTGAATACGTTACTCAAACTGCAATCGAGCATTTGGTTGTTGGTGCCTCAGCAAAAACCGGCTTTCTTAGATTCAAAGCATCAGATATTCCAGGAAACATATCAAAGGCGGCACCAGACTTTTGTAATGTATATGTTGTATCCAAGCAAAAACTCCAATCAAGGAGGGCAGCCTCACGTCCTGCCCCGGCTGTCTCACCCCTACGCAACCCAGCTCGTAACCACACCACAAGCCCAAAGTCAGAGCCGTACGTCTTCCCTCCTTCTGGTAACAAAGGAAATGAAAAGCCACCAGAACCACCACGTCAATCCCACGATGAAATGGACTTCTTCAGGTCTCCGTTTACTAGGAAAGGCCCGAACGGGAAGTCATATGCAGATCTCCCTATGCCGGATACTGACATATCTTTTGTTAGCTCTGGAAGGCCGAGCATTGACCGTATCTTCCCTGCATTTTATAACGAAAACCTAGATTCAGGAAGAGTCACCCCACCACGAATGTCGAACAGCACGGAAGTAGACTTGAACCACAGCTTTGAGTCTATGCAGTACTTTGGACGGAGGTCCGTGGATGTCAGCTCCTCTCCACCCACATTCTCTTCGGTTTCTCAGGATAGCGATAGGTTATCCTCTGCATCAATGGAAGACATGGAGGCTGAAATGAGAAGGCTAAAGCTGGAGCTCAAGCAAACTATGGAAATGTATAGCACTGCCTGTAAAGAGGCACTCTCGGCGAAGCAAAAGGCTGTAGAGCTCCAGCGCTGGAAATTAGAAGAGGAAAGGAGATTGGAAGAGGCGCGGCTAGCTGAGGAAACCGCAATGGCTATTGTAGAAAAGGAGAGAGCTAAGTCCAGAGCAGCCATTGAGGCTGCTGAAGCAGCTAAGAGGATTGCAGAACTGGAAGCACAAAAAAGAATCAATGCAGAAATGAAAGCACTCAGAGAACACGAAGAGAAAAAGAAGGCACTTAGCGCTCTGCAACAGTCCGATGTCAGGTATAGGAAGTACTCAATTGAAGAGATTGAGGCTGCCACAGAGTTCTTTACAGAATCCCGCAAGATTGGAGAAGGAGGTTACGGTCCTGTATATAGGTGTAATCTAGACCATACTCCTGTTGCAATTAAAGTTTTACGTCCAGATGCGGCACAAGGAAGATCACAGTTCAACCAAGAGGTTGAAGTATTGAGCTCTATACGGCATCCTAACATGGTTCTTCTGCTGGGAGCCTGCCCAGAGTACGGATGCTTGGTTTATGAGTACATGGCTAATGGGAGCTTGGAAGACCGTCTTCTTCGTAGAGGAAACAGTCCAATTCTCTCTTGGCAACTTAGATTTAAGATTGCAGCTGAAATTGGGACAGGCTTGCTATTCCTCCACCAAGCCAAACCAGAGCCACTAGTTCACCGTGACTTAAAGCCCGCTAACATCTTGCTTGACCGCAACTATGTGAGCAAGATCAGTGATGTTGGCTTGGCCAGGCTTGTGCCCCCATCTGTTGCTGACACCGTGACACAGTATCGCATGACATCAACAGCTGGCACGTTCTGCTACATAGATCCTGAGTATCAGCAAACCGGAATGCTTGGTGTTAAGTCTGATGTGTACTCTCTTGGGATTATGTTTCTGCAAATGATCACCGCGAAGCCGGCAATGGGATTGACTCACCATGTTGAAAGGGCTATTGAGAAGGGGACTTTCGAAGATATGCTGGATCCATCAGTGCCAGATTGGCCGGTTCAAGACACATTGAAGTTTGCCAAGCTGGCTCTCCAGTGTGCAGAGTTGAGGAGAAAAGACAGGCCGGACCTTCGAAAGGTTATCCTGCCAGAACTTAACAGATTGAGAGGGATTGCTGAAGAGACAATGCACCCAACACTGATGGCTGGTGGTCATAGCCCCTCATCCGAACACAGCCAAGTTTCTGTACAGCTAGAGGGGAGTGAACCAGAAACAAGATCTGCTGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Thioredoxin_8 Usp

Protein Analysis

1181

Amino Acids

132.67

Weight (kDa)

6.37

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AhpC-TSA PF00578 24 - 112 7.1e-06 AhpC/TSA family
Thioredoxin_8 PF13905 34 - 125 2.7e-22 Thioredoxin-like
AhpC-TSA PF00578 181 - 287 4.7e-06 AhpC/TSA family
Thioredoxin_8 PF13905 194 - 287 2.7e-24 Thioredoxin-like
C1_2 PF03107 334 - 375 1.6e-06 C1 domain
Pkinase PF00069 872 - 1129 1.4e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 875 - 1130 7.1e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2031
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 4 cut(s) 79, 902, 1616, 1684
AccB7I CCANNNNNTGG 2 cut(s) 538, 1447
AccI GTMKAC 1 cut(s) 2091
AccII CGCG 2 cut(s) 2371, 3212
AclWI GGATC 5 cut(s) 941, 1460, 3122, 3278, 3291
AcoI YGGCCR 3 cut(s) 1029, 3045, 3302
AcuI CTGAAG 4 cut(s) 63, 1888, 2466, 3447
AfeI AGCGCT 2 cut(s) 2333, 2545
AflII CTTAAG 1 cut(s) 1016
AflIII ACRYGT 2 cut(s) 324, 1511
AhlI ACTAGT 2 cut(s) 746, 2968
AjiI CACGTC 2 cut(s) 1751, 1877
AjnI CCWGG 4 cut(s) 777, 1336, 1663, 3047
AjuI GAANNNNNNNTTGG 4 cut(s) 381, 413, 1335, 1367
Alw21I GWGCWC 7 cut(s) 610, 669, 945, 1306, 2256, 2328, 2762
Alw44I GTGCAC 1 cut(s) 606
AlwI GGATC 5 cut(s) 941, 1460, 3122, 3278, 3291
Ama87I CYCGRG 1 cut(s) 1476
Aor51HI AGCGCT 2 cut(s) 2333, 2545
ApaLI GTGCAC 1 cut(s) 606
ApeKI GCWGC 7 cut(s) 15, 1742, 2429, 2441, 2450, 2600, 2915
ArsI GACNNNNNNTTYG 6 cut(s) 783, 815, 1925, 1957, 1964, 1996
AseI ATTAAT 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 79
AspLEI GCGC 3 cut(s) 2334, 2371, 2546
AspS9I GGNCC 6 cut(s) 848, 1438, 1928, 2136, 2651, 3376
AsuC2I CCSGG 3 cut(s) 1477, 1478, 1760
AsuHPI GGTGA 8 cut(s) 80, 181, 692, 1761, 2050, 2966, 3199, 3227
AsuII TTCGAA 3 cut(s) 693, 3270, 3382
AsuNHI GCTAGC 1 cut(s) 2374
AvaI CYCGRG 1 cut(s) 1476
AvaII GGWCC 5 cut(s) 848, 1438, 2136, 2651, 3376
BaeGI GKGCMC 3 cut(s) 291, 610, 3060
BaeI ACNNNNGTAYC 4 cut(s) 63, 96, 551, 584
BalI TGGCCA 2 cut(s) 1031, 3047
BamHI GGATCC 1 cut(s) 3283
BanI GGYRCC 4 cut(s) 79, 902, 1616, 1684
BanII GRGCYC 6 cut(s) 620, 669, 945, 2256, 2328, 2762
BbsI GAAGAC 8 cut(s) 110, 396, 417, 1554, 1819, 2223, 2852, 2859
Bbv12I GWGCWC 7 cut(s) 610, 669, 945, 1306, 2256, 2328, 2762
BbvCI CCTCAGC 3 cut(s) 162, 1621, 2379
BbvI GCAGC 7 cut(s) 27, 1754, 2428, 2441, 2462, 2587, 2927
BccI CCATC 7 cut(s) 355, 365, 542, 1021, 3070, 3295, 3445
BceAI ACGGC 3 cut(s) 612, 1804, 2783
BciT130I CCWGG 4 cut(s) 779, 1338, 1665, 3049
BciVI GTATCC 2 cut(s) 1723, 1960
BclI TGATCA 2 cut(s) 682, 3204
BcnI CCSGG 3 cut(s) 1477, 1478, 1760
BcuI ACTAGT 2 cut(s) 746, 2968
BfmI CTRYAG 1 cut(s) 2319
BfoI RGCGCY 2 cut(s) 2335, 2547
BfrI CTTAAG 1 cut(s) 1016
BfuI GTATCC 2 cut(s) 1723, 1960
BglI GCCNNNNNGGC 3 cut(s) 2438, 3301, 3335
BglII AGATCT 2 cut(s) 1951, 3527
BmcAI AGTACT 2 cut(s) 2123, 2579
Bme1390I CCNGG 7 cut(s) 779, 1338, 1477, 1478, 1665, 1760, 3049
Bme18I GGWCC 5 cut(s) 848, 1438, 2136, 2651, 3376
BmeT110I CYCGRG 1 cut(s) 1476
BmgBI CACGTC 2 cut(s) 1751, 1877
BmgT120I GGNCC 6 cut(s) 848, 1438, 1928, 2136, 2651, 3376
BmiI GGNNCC 8 cut(s) 81, 617, 904, 1439, 1618, 1686, 2797, 3285
BmrFI CCNGG 7 cut(s) 779, 1338, 1477, 1478, 1665, 1760, 3049
BmsI GCATC 9 cut(s) 286, 351, 415, 526, 1661, 2216, 2701, 2779, 2804
BmtI GCTAGC 1 cut(s) 2378
BoxI GACNNNNGTC 1 cut(s) 2134
BpiI GAAGAC 8 cut(s) 110, 396, 417, 1554, 1819, 2223, 2852, 2859
BplI GAGNNNNNCTC 2 cut(s) 2285, 2317
BpmI CTGGAG 4 cut(s) 634, 2270, 2312, 3326
Bpu10I CCTNAGC 3 cut(s) 162, 1621, 2379
Bpu14I TTCGAA 3 cut(s) 693, 3270, 3382
BpuEI CTTGAG 2 cut(s) 969, 2240
BpuMI CCSGG 3 cut(s) 1477, 1478, 1760
BsaAI YACGTR 1 cut(s) 1514
BsaBI GATNNNNATC 3 cut(s) 300, 1104, 1110
BsaI GGTCTC 5 cut(s) 714, 895, 1149, 1527, 1914
BsaJI CCNNGG 8 cut(s) 275, 758, 885, 906, 1159, 1476, 1758, 2139
BsaWI WCCGGW 3 cut(s) 65, 677, 3146
Bse118I RCCGGY 3 cut(s) 1631, 3217, 3304
Bse1I ACTGG 3 cut(s) 617, 2475, 3343
Bse3DI GCAATG 3 cut(s) 974, 2397, 3228
Bse8I GATNNNNATC 3 cut(s) 300, 1104, 1110
BseBI CCWGG 4 cut(s) 779, 1338, 1665, 3049
BseDI CCNNGG 8 cut(s) 275, 758, 885, 906, 1159, 1476, 1758, 2139
BseGI GGATG 9 cut(s) 342, 674, 699, 1111, 1356, 2149, 2770, 2819, 3473
BseJI GATNNNNATC 3 cut(s) 300, 1104, 1110
BseMI GCAATG 3 cut(s) 974, 2397, 3228
BseMII CTCAG 8 cut(s) 176, 1403, 1635, 2195, 2370, 2524, 3123, 3525
BseNI ACTGG 3 cut(s) 617, 2475, 3343
BseRI GAGGAG 3 cut(s) 972, 2143, 3373
BseSI GKGCMC 3 cut(s) 291, 610, 3060
BseXI GCAGC 7 cut(s) 27, 1754, 2428, 2441, 2462, 2587, 2927
BseYI CCCAGC 2 cut(s) 1783, 2791
BsgI GTGCAG 1 cut(s) 3369
Bsh1236I CGCG 2 cut(s) 2371, 3212
BshNI GGYRCC 4 cut(s) 79, 902, 1616, 1684
BsiHKAI GWGCWC 7 cut(s) 610, 669, 945, 1306, 2256, 2328, 2762
BsiHKCI CYCGRG 1 cut(s) 1476
BsiWI CGTACG 1 cut(s) 1821
BslFI GGGAC 4 cut(s) 1298, 1451, 2941, 3277
BsmFI GGGAC 4 cut(s) 1298, 1451, 2941, 3277
BsmI GAATGC 3 cut(s) 1375, 1396, 3156
Bso31I GGTCTC 5 cut(s) 714, 895, 1149, 1527, 1914
BsoBI CYCGRG 1 cut(s) 1476
Bsp119I TTCGAA 3 cut(s) 693, 3270, 3382
Bsp1407I TGTACA 1 cut(s) 3496
Bsp19I CCATGG 3 cut(s) 275, 758, 885
BspCNI CTCAG 8 cut(s) 175, 1402, 1634, 2194, 2371, 2523, 3124, 3526
BspFNI CGCG 2 cut(s) 2371, 3212
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 8 cut(s) 81, 617, 904, 1439, 1618, 1686, 2797, 3285
BspOI GCTAGC 1 cut(s) 2378
BspPI GGATC 5 cut(s) 941, 1460, 3122, 3278, 3291
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 3 cut(s) 693, 3270, 3382
BspT107I GGYRCC 4 cut(s) 79, 902, 1616, 1684
BspTI CTTAAG 1 cut(s) 1016
BspTNI GGTCTC 5 cut(s) 714, 895, 1149, 1527, 1914
BsrDI GCAATG 3 cut(s) 974, 2397, 3228
BsrFI RCCGGY 3 cut(s) 1631, 3217, 3304
BsrGI TGTACA 1 cut(s) 3496
BsrI ACTGG 3 cut(s) 617, 2475, 3343
BssAI RCCGGY 3 cut(s) 1631, 3217, 3304
BssECI CCNNGG 8 cut(s) 275, 758, 885, 906, 1159, 1476, 1758, 2139
BssT1I CCWWGG 4 cut(s) 275, 758, 885, 906
Bst2UI CCWGG 4 cut(s) 779, 1338, 1665, 3049
BstAFI CTTAAG 1 cut(s) 1016
BstAPI GCANNNNNTGC 1 cut(s) 3117
BstAUI TGTACA 1 cut(s) 3496
BstBAI YACGTR 1 cut(s) 1514
BstBI TTCGAA 3 cut(s) 693, 3270, 3382
BstDSI CCRYGG 5 cut(s) 275, 758, 885, 1159, 2139
BstENI CCTNNNNNAGG 1 cut(s) 3383
BstF5I GGATG 9 cut(s) 342, 674, 699, 1111, 1356, 2149, 2770, 2819, 3473
BstFNI CGCG 2 cut(s) 2371, 3212
BstH2I RGCGCY 2 cut(s) 2335, 2547
BstHHI GCGC 3 cut(s) 2334, 2371, 2546
BstNI CCWGG 4 cut(s) 779, 1338, 1665, 3049
BstNSI RCATGY 3 cut(s) 274, 328, 757
BstPAI GACNNNNGTC 1 cut(s) 2134
BstSCI CCNGG 7 cut(s) 777, 1336, 1475, 1476, 1663, 1758, 3047
BstSFI CTRYAG 1 cut(s) 2319
BstSLI GKGCMC 3 cut(s) 291, 610, 3060
BstUI CGCG 2 cut(s) 2371, 3212
BstV1I GCAGC 7 cut(s) 27, 1754, 2428, 2441, 2462, 2587, 2927
BstV2I GAAGAC 8 cut(s) 110, 396, 417, 1554, 1819, 2223, 2852, 2859
BstX2I RGATCY 4 cut(s) 1951, 3127, 3283, 3527
BstYI RGATCY 4 cut(s) 1951, 3127, 3283, 3527
BsuI GTATCC 2 cut(s) 1723, 1960
BtgI CCRYGG 5 cut(s) 275, 758, 885, 1159, 2139
BtrI CACGTC 2 cut(s) 1751, 1877
BtsCI GGATG 9 cut(s) 342, 674, 699, 1111, 1356, 2149, 2770, 2819, 3473
BtsI GCAGTG 4 cut(s) 12, 291, 1275, 2280
CciI TCATGA 1 cut(s) 669
CfoI GCGC 3 cut(s) 2334, 2371, 2546
Cfr10I RCCGGY 3 cut(s) 1631, 3217, 3304
Cfr13I GGNCC 6 cut(s) 848, 1438, 1928, 2136, 2651, 3376
Cfr9I CCCGGG 1 cut(s) 1476
CseI GACGC 1 cut(s) 523
EaeI YGGCCR 3 cut(s) 1029, 3045, 3302
Ecl136II GAGCTC 5 cut(s) 667, 943, 2254, 2326, 2760
Eco130I CCWWGG 4 cut(s) 275, 758, 885, 906
Eco147I AGGCCT 2 cut(s) 594, 911
Eco24I GRGCYC 6 cut(s) 620, 669, 945, 2256, 2328, 2762
Eco31I GGTCTC 5 cut(s) 714, 895, 1149, 1527, 1914
Eco47I GGWCC 5 cut(s) 848, 1438, 2136, 2651, 3376
Eco47III AGCGCT 2 cut(s) 2333, 2545
Eco53kI GAGCTC 5 cut(s) 667, 943, 2254, 2326, 2760
Eco57I CTGAAG 4 cut(s) 63, 1888, 2466, 3447
Eco88I CYCGRG 1 cut(s) 1476
EcoICRI GAGCTC 5 cut(s) 667, 943, 2254, 2326, 2760
EcoNI CCTNNNNNAGG 1 cut(s) 3383
EcoRII CCWGG 4 cut(s) 777, 1336, 1663, 3047
EcoT14I CCWWGG 4 cut(s) 275, 758, 885, 906
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 6 cut(s) 620, 669, 945, 2256, 2328, 2762
ErhI CCWWGG 4 cut(s) 275, 758, 885, 906
FalI AAGNNNNNCTT 2 cut(s) 2844, 2876
FaqI GGGAC 4 cut(s) 1298, 1451, 2941, 3277
FauI CCCGC 2 cut(s) 2633, 2998
FauNDI CATATG 2 cut(s) 1039, 1945
FbaI TGATCA 2 cut(s) 682, 3204
FblI GTMKAC 1 cut(s) 2091
FokI GGATG 9 cut(s) 329, 661, 686, 1098, 1343, 2156, 2757, 2826, 3460
FriOI GRGCYC 6 cut(s) 620, 669, 945, 2256, 2328, 2762
GlaI GCGC 3 cut(s) 2333, 2370, 2545
GsaI CCCAGC 2 cut(s) 1787, 2795
GsuI CTGGAG 4 cut(s) 634, 2270, 2312, 3326
HaeII RGCGCY 2 cut(s) 2335, 2547
HgaI GACGC 1 cut(s) 523
HhaI GCGC 3 cut(s) 2334, 2371, 2546
Hin6I GCGC 3 cut(s) 2332, 2369, 2544
HinP1I GCGC 3 cut(s) 2332, 2369, 2544
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HphI GGTGA 8 cut(s) 80, 181, 692, 1761, 2050, 2966, 3199, 3227
Hpy166II GTNNAC 9 cut(s) 513, 608, 921, 1237, 1917, 2092, 2974, 3175, 3515
Hpy8I GTNNAC 9 cut(s) 513, 608, 921, 1237, 1917, 2092, 2974, 3175, 3515
HpyCH4IV ACGT 7 cut(s) 1513, 1581, 1750, 1824, 1876, 2704, 3113
HpySE526I ACGT 7 cut(s) 1513, 1581, 1750, 1824, 1876, 2704, 3113
HspAI GCGC 3 cut(s) 2332, 2369, 2544
KpnI GGTACC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 3217
KroNI GCCGGC 1 cut(s) 3219
Ksp22I TGATCA 2 cut(s) 682, 3204
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 7 cut(s) 419, 615, 2156, 2251, 2331, 2795, 2844
Lsp1109I GCAGC 7 cut(s) 27, 1754, 2428, 2441, 2462, 2587, 2927
LweI GCATC 9 cut(s) 286, 351, 415, 526, 1661, 2216, 2701, 2779, 2804
MaeII ACGT 7 cut(s) 1513, 1581, 1750, 1824, 1876, 2704, 3113
MfeI CAATTG 2 cut(s) 1317, 2583
MflI RGATCY 4 cut(s) 1951, 3127, 3283, 3527
MlsI TGGCCA 2 cut(s) 1031, 3047
MluNI TGGCCA 2 cut(s) 1031, 3047
MlyI GAGTC 4 cut(s) 1544, 2064, 2120, 3226
MmeI TCCRAC 1 cut(s) 379
Mox20I TGGCCA 2 cut(s) 1031, 3047
Mph1103I ATGCAT 1 cut(s) 430
MroNI GCCGGC 1 cut(s) 3217
MscI TGGCCA 2 cut(s) 1031, 3047
MseI TTAA 8 cut(s) 362, 404, 1017, 2694, 2907, 2984, 3162, 3405
MslI CAYNNNNRTG 2 cut(s) 821, 1042
Msp20I TGGCCA 2 cut(s) 1031, 3047
MspA1I CMGCKG 3 cut(s) 166, 2918, 3107
MspCI CTTAAG 1 cut(s) 1016
MspR9I CCNGG 7 cut(s) 779, 1338, 1477, 1478, 1665, 1760, 3049
MunI CAATTG 2 cut(s) 1317, 2583
Mva1269I GAATGC 3 cut(s) 1375, 1396, 3156
MvaI CCWGG 4 cut(s) 779, 1338, 1665, 3049
MvnI CGCG 2 cut(s) 2371, 3212
NaeI GCCGGC 1 cut(s) 3219
NciI CCSGG 3 cut(s) 1477, 1478, 1760
NcoI CCATGG 3 cut(s) 275, 758, 885
NdeI CATATG 2 cut(s) 1039, 1945
NgoMIV GCCGGC 1 cut(s) 3217
NheI GCTAGC 1 cut(s) 2374
NlaIV GGNNCC 8 cut(s) 81, 617, 904, 1439, 1618, 1686, 2797, 3285
NmeAIII GCCGAG 2 cut(s) 2025, 2282
NmuCI GTSAC 5 cut(s) 68, 320, 2056, 2978, 3079
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 3 cut(s) 274, 328, 757
NspV TTCGAA 3 cut(s) 693, 3270, 3382
PaeI GCATGC 2 cut(s) 274, 757
PagI TCATGA 1 cut(s) 669
PceI AGGCCT 2 cut(s) 594, 911
PciI ACATGT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 657
PctI GAATGC 3 cut(s) 1375, 1396, 3156
PdiI GCCGGC 1 cut(s) 3219
PfeI GAWTC 7 cut(s) 38, 807, 826, 1644, 2045, 2487, 2621
Pfl23II CGTACG 1 cut(s) 1821
PflMI CCANNNNNTGG 2 cut(s) 538, 1447
PfoI TCCNGGA 1 cut(s) 1663
PleI GAGTC 4 cut(s) 1544, 2063, 2119, 3226
PpsI GAGTC 4 cut(s) 1544, 2063, 2119, 3226
Ppu21I YACGTR 1 cut(s) 1514
PscI ACATGT 1 cut(s) 324
PshAI GACNNNNGTC 1 cut(s) 2134
PshBI ATTAAT 1 cut(s) 404
PsiI TTATAA 1 cut(s) 2031
Psp124BI GAGCTC 5 cut(s) 669, 945, 2256, 2328, 2762
Psp6I CCWGG 4 cut(s) 777, 1336, 1663, 3047
PspFI CCCAGC 2 cut(s) 1783, 2791
PspGI CCWGG 4 cut(s) 777, 1336, 1663, 3047
PspLI CGTACG 1 cut(s) 1821
PspN4I GGNNCC 8 cut(s) 81, 617, 904, 1439, 1618, 1686, 2797, 3285
PspPI GGNCC 6 cut(s) 848, 1438, 1928, 2136, 2651, 3376
PsuI RGATCY 4 cut(s) 1951, 3127, 3283, 3527
PvuII CAGCTG 3 cut(s) 166, 2918, 3107
RseI CAYNNNNRTG 2 cut(s) 821, 1042
SacI GAGCTC 5 cut(s) 669, 945, 2256, 2328, 2762
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 8 cut(s) 362, 404, 1017, 2694, 2907, 2984, 3162, 3405
Sau96I GGNCC 6 cut(s) 848, 1438, 1928, 2136, 2651, 3376
ScaI AGTACT 2 cut(s) 2123, 2579
SchI GAGTC 4 cut(s) 1544, 2064, 2120, 3226
ScrFI CCNGG 7 cut(s) 779, 1338, 1477, 1478, 1665, 1760, 3049
SfaNI GCATC 9 cut(s) 286, 351, 415, 526, 1661, 2216, 2701, 2779, 2804
SfcI CTRYAG 1 cut(s) 2319
SfuI TTCGAA 3 cut(s) 693, 3270, 3382
SinI GGWCC 5 cut(s) 848, 1438, 2136, 2651, 3376
SmaI CCCGGG 1 cut(s) 1478
SmiMI CAYNNNNRTG 2 cut(s) 821, 1042
SmlI CTYRAG 3 cut(s) 984, 1016, 2255
SmoI CTYRAG 3 cut(s) 984, 1016, 2255
SpeI ACTAGT 2 cut(s) 746, 2968
SphI GCATGC 2 cut(s) 274, 757
SseBI AGGCCT 2 cut(s) 594, 911
SstI GAGCTC 5 cut(s) 669, 945, 2256, 2328, 2762
StuI AGGCCT 2 cut(s) 594, 911
StyD4I CCNGG 7 cut(s) 777, 1336, 1475, 1476, 1663, 1758, 3047
StyI CCWWGG 4 cut(s) 275, 758, 885, 906
TaiI ACGT 7 cut(s) 1516, 1584, 1753, 1827, 1879, 2707, 3116
TaqII GACCGA 2 cut(s) 732, 865
TatI WGTACW 6 cut(s) 1540, 2121, 2577, 2829, 3174, 3496
TauI GCSGC 4 cut(s) 470, 1685, 2374, 2717
TfiI GAWTC 7 cut(s) 38, 807, 826, 1644, 2045, 2487, 2621
Tru1I TTAA 8 cut(s) 362, 404, 1017, 2694, 2907, 2984, 3162, 3405
Tru9I TTAA 8 cut(s) 362, 404, 1017, 2694, 2907, 2984, 3162, 3405
TseFI GTSAC 5 cut(s) 68, 320, 2056, 2978, 3079
TseI GCWGC 7 cut(s) 15, 1742, 2429, 2441, 2450, 2600, 2915
Tsp45I GTSAC 5 cut(s) 68, 320, 2056, 2978, 3079
TspGWI ACGGA 6 cut(s) 1487, 1902, 2099, 2128, 2146, 2826
TspMI CCCGGG 1 cut(s) 1476
Van91I CCANNNNNTGG 2 cut(s) 538, 1447
Vha464I CTTAAG 1 cut(s) 1016
VneI GTGCAC 1 cut(s) 606
VpaK11BI GGWCC 5 cut(s) 848, 1438, 2136, 2651, 3376
VspI ATTAAT 1 cut(s) 404
XagI CCTNNNNNAGG 1 cut(s) 3383
XbaI TCTAGA 1 cut(s) 2671
XceI RCATGY 3 cut(s) 274, 328, 757
XcmI CCANNNNNNNNNTGG 2 cut(s) 1231, 2889
XmaI CCCGGG 1 cut(s) 1476
XmiI GTMKAC 1 cut(s) 2091
ZrmI AGTACT 2 cut(s) 2123, 2579
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.