MD15G1105200.v1.1

Nucleoredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005579
Physical Location & Seq
Forward (+)
7366594 .. 7367366
773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1105200.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGAAGACTTGGTTGGACTGATTGAAGACTATGGAGCTGCTGCATTTCCATTCAATAAGAAGAGAAGAGAAGAGCTAACGGCTGCCGATGAAGCAAAGCGTGAAGGGAGAAAACTCGAAGAGTTGTTGACGCATCAAGGACGAAACCATTTTGGTGCTCATTGGAGCGCTCCTTGCCGTGCTTTCACTTCCAAACTCATAGAAGGATACGAAGAGCTCATAACCTCCGGTGATCAAGACTTCGAAATCATCCTAGTCTCCATAGACCGAGACCTCAAAGAGTTTGAACTCAACACGAGTAGCATGCCATGGCTTGCCATACCCTACCAGGATAAAACAAGGCAAGACCTTTGTGGAATCTTCGACATCAAAGTGATTCCGGCTCTGGTTCTGATAGGACCGGATGGGAAGGCTATTAGCACAAATGGGAAGGCCATGATATCCTTGTACGGTGCCAAGGCCTTCTCGTTCACAGAGTCGAGGATCAAGGACCTCGAAGCAGCATTGAGAAACGAAGGAGAGGCATTGCCTCCTCAAGTGAAGGACTTGAAACATGAACATATGCTTGAGTTGGAGATGGCCAAAGCATATGCGTGTGATTATTACAAAAAACAAGGGAGGTTTTGGGCATTTTGTTGTGGTGTTTGTGATTATGATCTTCATCCAAATTGCGTAGAAAAATCGTTTGTAGAAATGGGTTTTACGAATTATGAAATTTGTATATCCTAA

Protein Analysis

243

Amino Acids

27.44

Weight (kDa)

5.1

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin_8 PF13905 51 - 137 3.5e-19 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 452
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 1 cut(s) 714
AfaI GTAC 1 cut(s) 449
AfeI AGCGCT 1 cut(s) 169
AgsI TTSAA 4 cut(s) 26, 55, 287, 550
AjnI CCWGG 1 cut(s) 327
AjuI GAANNNNNNNTTGG 1 cut(s) 29
AluBI AGCT 3 cut(s) 38, 76, 217
AluI AGCT 3 cut(s) 38, 76, 217
Alw21I GWGCWC 2 cut(s) 160, 219
Alw26I GTCTC 2 cut(s) 262, 264
AlwI GGATC 1 cut(s) 491
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 3 cut(s) 432, 459, 579
ApeKI GCWGC 4 cut(s) 38, 41, 83, 500
ApoI RAATTY 1 cut(s) 714
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 398, 490
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 243
AvaII GGWCC 2 cut(s) 398, 490
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 1 cut(s) 452
BanII GRGCYC 1 cut(s) 219
BauI CACGAG 1 cut(s) 295
BbsI GAAGAC 2 cut(s) 12, 33
Bbv12I GWGCWC 2 cut(s) 160, 219
BbvI GCAGC 4 cut(s) 25, 28, 70, 512
BccI CCATC 2 cut(s) 398, 571
BceAI ACGGC 2 cut(s) 96, 162
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 1 cut(s) 329
BciVI GTATCC 1 cut(s) 200
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 262, 264
BfaI CTAG 1 cut(s) 254
BfoI RGCGCY 1 cut(s) 171
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 4 cut(s) 39, 42, 84, 501
BlsI GCNGC 4 cut(s) 40, 43, 85, 502
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 2 cut(s) 398, 490
BmgT120I GGNCC 2 cut(s) 398, 490
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 12, 33
Bpu14I TTCGAA 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 519, 587
BsaBI GATNNNNATC 2 cut(s) 654, 660
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 308, 456
BsaWI WCCGGW 2 cut(s) 227, 400
Bse3DI GCAATG 1 cut(s) 524
Bse8I GATNNNNATC 2 cut(s) 654, 660
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 2 cut(s) 308, 456
BseGI GGATG 3 cut(s) 249, 409, 661
BseJI GATNNNNATC 2 cut(s) 654, 660
BseMI GCAATG 1 cut(s) 524
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 4 cut(s) 25, 28, 70, 512
BshFI GGCC 3 cut(s) 434, 461, 581
BshNI GGYRCC 1 cut(s) 452
BsiHKAI GWGCWC 2 cut(s) 160, 219
BsiSI CCGG 3 cut(s) 228, 380, 401
BsmAI GTCTC 2 cut(s) 262, 264
BsnI GGCC 3 cut(s) 434, 461, 581
Bso31I GGTCTC 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 160, 219
Bsp143I GATC 3 cut(s) 232, 483, 655
Bsp19I CCATGG 1 cut(s) 308
BspANI GGCC 3 cut(s) 434, 461, 581
BspLI GGNNCC 1 cut(s) 454
BspPI GGATC 1 cut(s) 491
BspQI GCTCTTC 2 cut(s) 66, 207
BspT104I TTCGAA 1 cut(s) 243
BspT107I GGYRCC 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 524
BssECI CCNNGG 2 cut(s) 308, 456
BssMI GATC 3 cut(s) 232, 483, 655
BssSI CACGAG 1 cut(s) 295
BssT1I CCWWGG 2 cut(s) 308, 456
Bst2BI CACGAG 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 5 cut(s) 56, 61, 66, 114, 207
BstBI TTCGAA 1 cut(s) 243
BstC8I GCNNGC 2 cut(s) 305, 315
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 3 cut(s) 249, 409, 661
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 3 cut(s) 235, 486, 658
BstMAI GTCTC 2 cut(s) 262, 264
BstMBI GATC 3 cut(s) 232, 483, 655
BstMWI GCNNNNNNNGC 2 cut(s) 92, 174
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 327
BstV1I GCAGC 4 cut(s) 25, 28, 70, 512
BstV2I GAAGAC 2 cut(s) 12, 33
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 3 cut(s) 434, 461, 581
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 3 cut(s) 249, 409, 661
Cac8I GCNNGC 2 cut(s) 305, 315
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 398, 490
CseI GACGC 1 cut(s) 139
Csp6I GTAC 1 cut(s) 448
CviAII CATG 4 cut(s) 304, 309, 436, 554
CviQI GTAC 1 cut(s) 448
DpnI GATC 3 cut(s) 234, 485, 657
DpnII GATC 3 cut(s) 232, 483, 655
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 5 cut(s) 56, 61, 66, 114, 207
EarI CTCTTC 5 cut(s) 56, 61, 66, 114, 207
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 308, 456
Eco147I AGGCCT 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 2 cut(s) 398, 490
Eco47III AGCGCT 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 327
EcoRV GATATC 1 cut(s) 441
EcoT14I CCWWGG 2 cut(s) 308, 456
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 2 cut(s) 308, 456
FaeI CATG 4 cut(s) 307, 312, 439, 557
FatI CATG 4 cut(s) 303, 308, 435, 553
FauNDI CATATG 2 cut(s) 561, 589
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 4 cut(s) 39, 42, 84, 501
FokI GGATG 3 cut(s) 236, 416, 648
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 4 cut(s) 39, 42, 84, 501
FspBI CTAG 1 cut(s) 254
GlaI GCGC 1 cut(s) 169
GluI GCNGC 4 cut(s) 39, 42, 84, 501
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 3 cut(s) 434, 461, 581
HapII CCGG 3 cut(s) 228, 380, 401
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 170
Hin1II CATG 4 cut(s) 307, 312, 439, 557
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 3 cut(s) 357, 376, 476
HpaII CCGG 3 cut(s) 228, 380, 401
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 129, 471
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 2 cut(s) 129, 471
HpyAV CCTTC 7 cut(s) 98, 197, 403, 424, 472, 509, 535
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 174
Hsp92II CATG 4 cut(s) 307, 312, 439, 557
HspAI GCGC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 3 cut(s) 232, 483, 655
LguI GCTCTTC 2 cut(s) 66, 207
LmnI GCTCC 3 cut(s) 35, 165, 175
LpnPI CCDG 6 cut(s) 241, 314, 341, 371, 393, 414
Lsp1109I GCAGC 4 cut(s) 25, 28, 70, 512
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 254
MalI GATC 3 cut(s) 234, 485, 657
MboI GATC 3 cut(s) 232, 483, 655
MboII GAAGA 9 cut(s) 17, 38, 73, 78, 83, 131, 224, 352, 650
MhlI GDGCHC 2 cut(s) 160, 219
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 3 cut(s) 667, 706, 714
MluNI TGGCCA 1 cut(s) 581
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 552
MnlI CCTC 8 cut(s) 235, 284, 474, 503, 514, 540, 543, 612
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
MslI CAYNNNNRTG 3 cut(s) 153, 371, 592
Msp20I TGGCCA 1 cut(s) 581
MspI CCGG 3 cut(s) 228, 380, 401
MspR9I CCNGG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 329
MwoI GCNNNNNNNGC 2 cut(s) 92, 174
NcoI CCATGG 1 cut(s) 308
NdeI CATATG 2 cut(s) 561, 589
NdeII GATC 3 cut(s) 232, 483, 655
NlaIII CATG 4 cut(s) 307, 312, 439, 557
NlaIV GGNNCC 1 cut(s) 454
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 1 cut(s) 243
PaeI GCATGC 1 cut(s) 307
PceI AGGCCT 1 cut(s) 461
PciSI GCTCTTC 2 cut(s) 66, 207
PfeI GAWTC 2 cut(s) 357, 376
PkrI GCNGC 4 cut(s) 40, 43, 85, 502
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PpuMI RGGWCCY 1 cut(s) 490
Psp124BI GAGCTC 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 1 cut(s) 327
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 2 cut(s) 398, 490
PspPPI RGGWCCY 1 cut(s) 490
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 3 cut(s) 153, 371, 592
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 2 cut(s) 66, 207
SatI GCNGC 4 cut(s) 39, 42, 84, 501
Sau3AI GATC 3 cut(s) 232, 483, 655
Sau96I GGNCC 2 cut(s) 398, 490
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 2 cut(s) 160, 219
SetI ASST 8 cut(s) 40, 78, 219, 227, 276, 351, 495, 623
SfaNI GCATC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 243
SinI GGWCC 2 cut(s) 398, 490
SmiMI CAYNNNNRTG 3 cut(s) 153, 371, 592
SmlI CTYRAG 2 cut(s) 534, 566
SmoI CTYRAG 2 cut(s) 534, 566
SphI GCATGC 1 cut(s) 307
Sse9I AATT 3 cut(s) 667, 706, 714
SseBI AGGCCT 1 cut(s) 461
SspMI CTAG 1 cut(s) 254
SstI GAGCTC 1 cut(s) 219
StuI AGGCCT 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 2 cut(s) 308, 456
TaaI ACNGT 1 cut(s) 452
TaqI TCGA 5 cut(s) 117, 243, 363, 479, 495
TaqII GACCGA 1 cut(s) 282
TasI AATT 3 cut(s) 667, 706, 714
TfiI GAWTC 2 cut(s) 357, 376
TseI GCWGC 4 cut(s) 38, 41, 83, 500
TspDTI ATGAA 4 cut(s) 105, 570, 650, 726
VpaK11BI GGWCC 2 cut(s) 398, 490
XapI RAATTY 1 cut(s) 714
XceI RCATGY 1 cut(s) 307
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.