pycom08g10420

Ribonuclease H2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007200
Physical Location & Seq
Forward (+)
8722892 .. 8724050
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g10420.2

Sequence Viewer

Length: 438 bp
ATGGCGTGGTGGGAAGGGTTCGAAGAAACCCGTCTTCTCATCGCAGCAGATACTACTGAATCCGGAAACGACGTCGGACAATTGATTTCGCTCCGCCATCCCAAGTCCGGAAACAAAATGTGCTGCCTTTTTGTTAATGGGGTACTTCAGGAACTCAATTGGTTTAAGCAATCCTGCACTTTTTGGTGTCTGGGTGAATATGTTACTGCAGATGCTGGGTTATATACTGCCACTCCAGTTGACCCTGTCTTCATTTTCTTGCCCATTTTCGAAGAAGCTAGGATGAAGAAAGGGGACGTTCCTGGGAAGTTCAGGCTTTTAGATGACATAATCTTCATCGAAGGCTATCCGGGATACCATAAATTACTGTCCATTGCGGAGAACTGTTTGCAATTAGTTTGCGAAAAAAAAAGAAGTCGGATCCTCCAAATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2-Ydr279

Protein Analysis

146

Amino Acids

16.51

Weight (kDa)

5.39

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccIII TCCGGA 2 cut(s) 62, 107
AciI CCGC 2 cut(s) 94, 377
AclWI GGATC 2 cut(s) 415, 428
AcuI CTGAAG 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 107
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
AlwI GGATC 2 cut(s) 415, 428
AlwNI CAGNNNCTG 1 cut(s) 215
Aor13HI TCCGGA 2 cut(s) 62, 107
ApeKI GCWGC 2 cut(s) 44, 123
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 1 cut(s) 206
AsuII TTCGAA 2 cut(s) 21, 270
BamHI GGATCC 1 cut(s) 420
BbsI GAAGAC 2 cut(s) 26, 241
BbvI GCAGC 2 cut(s) 56, 110
BccI CCATC 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 303
BciVI GTATCC 1 cut(s) 347
BcnI CCSGG 1 cut(s) 351
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 207
BfuI GTATCC 1 cut(s) 347
BisI GCNGC 2 cut(s) 45, 124
BlsI GCNGC 2 cut(s) 46, 125
Bme1390I CCNGG 2 cut(s) 303, 351
BmiI GGNNCC 1 cut(s) 422
BmrFI CCNGG 2 cut(s) 303, 351
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 2 cut(s) 26, 241
BpmI CTGGAG 1 cut(s) 219
Bpu14I TTCGAA 2 cut(s) 21, 270
BpuMI CCSGG 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 302
BsaWI WCCGGW 2 cut(s) 62, 107
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 372
BseAI TCCGGA 2 cut(s) 62, 107
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 302
BseGI GGATG 2 cut(s) 97, 288
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 56, 110
BseYI CCCAGC 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 160
BsiSI CCGG 3 cut(s) 63, 108, 350
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 308
Bsp119I TTCGAA 2 cut(s) 21, 270
Bsp13I TCCGGA 2 cut(s) 62, 107
Bsp143I GATC 1 cut(s) 420
BspACI CCGC 2 cut(s) 94, 377
BspEI TCCGGA 2 cut(s) 62, 107
BspLI GGNNCC 1 cut(s) 422
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 2 cut(s) 415, 428
BspT104I TTCGAA 2 cut(s) 21, 270
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 1 cut(s) 420
BssNI GRCGYC 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 369, 386
BstACI GRCGYC 1 cut(s) 72
BstBI TTCGAA 2 cut(s) 21, 270
BstF5I GGATG 2 cut(s) 97, 288
BstKTI GATC 1 cut(s) 423
BstMBI GATC 1 cut(s) 420
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 301, 349
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 56, 110
BstV2I GAAGAC 2 cut(s) 26, 241
BstX2I RGATCY 1 cut(s) 420
BstYI RGATCY 1 cut(s) 420
BsuI GTATCC 1 cut(s) 347
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 97, 288
CaiI CAGNNNCTG 1 cut(s) 215
Csp6I GTAC 1 cut(s) 143
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviJI RGCY 3 cut(s) 278, 316, 345
CviKI_1 RGCY 3 cut(s) 278, 316, 345
CviQI GTAC 1 cut(s) 143
DpnI GATC 1 cut(s) 422
DpnII GATC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 301
FaiI YATR 5 cut(s) 201, 223, 225, 329, 360
FaqI GGGAC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 45, 124
FokI GGATG 2 cut(s) 84, 295
Fsp4HI GCNGC 2 cut(s) 45, 124
FspBI CTAG 1 cut(s) 279
GluI GCNGC 2 cut(s) 45, 124
GsaI CCCAGC 1 cut(s) 219
GsuI CTGGAG 1 cut(s) 219
HapII CCGG 3 cut(s) 63, 108, 350
Hin1I GRCGYC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 59
HpaII CCGG 3 cut(s) 63, 108, 350
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 77, 420
Hpy188III TCNNGA 3 cut(s) 63, 108, 149
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 2 cut(s) 74, 77
HpyAV CCTTC 2 cut(s) 8, 335
HpyCH4III ACNGT 2 cut(s) 369, 386
HpyCH4IV ACGT 2 cut(s) 72, 297
HpyCH4V TGCA 3 cut(s) 177, 209, 391
HpySE526I ACGT 2 cut(s) 72, 297
Hsp92I GRCGYC 1 cut(s) 72
Kpn2I TCCGGA 2 cut(s) 62, 107
Kzo9I GATC 1 cut(s) 420
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 56, 110
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 279
MaeII ACGT 2 cut(s) 72, 297
MaeIII GTNAC 1 cut(s) 202
MalI GATC 1 cut(s) 422
MboI GATC 1 cut(s) 420
MboII GAAGA 6 cut(s) 26, 35, 241, 284, 298, 325
MfeI CAATTG 2 cut(s) 80, 157
MflI RGATCY 1 cut(s) 420
MluCI AATT 5 cut(s) 80, 157, 362, 392, 429
MmeI TCCRAC 2 cut(s) 55, 398
MnlI CCTC 1 cut(s) 434
MroI TCCGGA 2 cut(s) 62, 107
MseI TTAA 2 cut(s) 135, 165
MspI CCGG 3 cut(s) 63, 108, 350
MspR9I CCNGG 2 cut(s) 303, 351
MunI CAATTG 2 cut(s) 80, 157
MvaI CCWGG 1 cut(s) 303
NciI CCSGG 1 cut(s) 351
NdeII GATC 1 cut(s) 420
NlaIV GGNNCC 1 cut(s) 422
NspV TTCGAA 2 cut(s) 21, 270
PfeI GAWTC 1 cut(s) 59
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 349
PkrI GCNGC 2 cut(s) 46, 125
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 215
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 422
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 1 cut(s) 420
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SaqAI TTAA 2 cut(s) 135, 165
SatI GCNGC 2 cut(s) 45, 124
Sau3AI GATC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 303, 351
SetI ASST 3 cut(s) 75, 280, 300
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 1 cut(s) 207
SfuI TTCGAA 2 cut(s) 21, 270
Sse9I AATT 5 cut(s) 80, 157, 362, 392, 429
SsiI CCGC 2 cut(s) 94, 377
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 301, 349
TaaI ACNGT 2 cut(s) 369, 386
TaiI ACGT 2 cut(s) 75, 300
TaqI TCGA 3 cut(s) 21, 270, 339
TasI AATT 5 cut(s) 80, 157, 362, 392, 429
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 135, 165
Tru9I TTAA 2 cut(s) 135, 165
TseI GCWGC 2 cut(s) 44, 123
TspDTI ATGAA 3 cut(s) 241, 299, 325
Tth111I GACNNNGTC 1 cut(s) 245
XspI CTAG 1 cut(s) 279
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.