MD08G1134800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006779
Physical Location & Seq
Reverse (-)
12811706 .. 12812337
632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1134800.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGCGGCTTCACCATCTCAGTCGGACCTTCAGCTCCAACTCCAATCGGAGTCCGAGTCTCCGATGTCATCCATCGTCTACGTGATTTCACAGCAAGTCCAGGGTGCGATGGAGAACATGTTTAAGATGATGAGCGAAACTGATGAGAACTCTGCCGGGATTTCGGAAGAGATCGAGAAGTGCAAGGAATCTGCTCTGGAGAAGAAGAAAGGCTTAGAGGAAGCCAGAGAGCAAGTCGAGAAGGCCGCTTATGCCGTTTTGGAGATGCTCAACAACCGCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.45

Weight (kDa)

4.47

Isoelectric Point (pI)

89.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 78
AciI CCGC 3 cut(s) 5, 246, 277
AcuI CTGAAG 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 117
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
Alw26I GTCTC 1 cut(s) 63
AoxI GGCC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 3
AvaII GGWCC 1 cut(s) 25
BccI CCATC 3 cut(s) 22, 80, 103
BceAI ACGGC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 101
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 63
BisI GCNGC 2 cut(s) 6, 246
BlsI GCNGC 2 cut(s) 7, 247
Bme1390I CCNGG 2 cut(s) 101, 157
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 101, 157
BmsI GCATC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 157
BsaAI YACGTR 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 100
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 104, 134
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 245
BsiSI CCGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 63
BsnI GGCC 1 cut(s) 245
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 3 cut(s) 5, 246, 277
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 82
BstDEI CTNAG 2 cut(s) 18, 214
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 1 cut(s) 174
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 99, 155
BsuRI GGCC 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 122
BtsCI GGATG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 25
CviAII CATG 1 cut(s) 118
CviJI RGCY 5 cut(s) 8, 34, 213, 224, 245
CviKI_1 RGCY 5 cut(s) 8, 34, 213, 224, 245
DdeI CTNAG 2 cut(s) 18, 214
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 121
FaiI YATR 3 cut(s) 119, 252, 283
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 1 cut(s) 117
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 2 cut(s) 6, 246
FokI GGATG 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 6, 246
GluI GCNGC 2 cut(s) 6, 246
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 1 cut(s) 245
HapII CCGG 1 cut(s) 156
Hin1II CATG 1 cut(s) 121
HinfI GANTC 3 cut(s) 50, 56, 188
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 5 cut(s) 25, 49, 55, 63, 166
Hpy188III TCNNGA 3 cut(s) 175, 197, 238
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 38, 235
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 18, 214
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 1 cut(s) 121
Kzo9I GATC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 39
LpnPI CCDG 5 cut(s) 86, 113, 169, 182, 238
LweI GCATC 1 cut(s) 255
MaeII ACGT 1 cut(s) 81
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 3 cut(s) 179, 214, 217
MlyI GAGTC 2 cut(s) 59, 65
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 1 cut(s) 211
MseI TTAA 1 cut(s) 123
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 101, 157
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 157
NdeII GATC 1 cut(s) 171
NlaIII CATG 1 cut(s) 121
NspI RCATGY 1 cut(s) 121
PciI ACATGT 1 cut(s) 117
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 2 cut(s) 7, 247
PleI GAGTC 2 cut(s) 58, 64
PpsI GAGTC 2 cut(s) 58, 64
Ppu21I YACGTR 1 cut(s) 82
PscI ACATGT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspPI GGNCC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 6, 246
Sau3AI GATC 1 cut(s) 171
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 2 cut(s) 59, 65
ScrFI CCNGG 2 cut(s) 101, 157
SetI ASST 3 cut(s) 30, 36, 84
SfaNI GCATC 1 cut(s) 255
SinI GGWCC 1 cut(s) 25
SsiI CCGC 3 cut(s) 5, 246, 277
StyD4I CCNGG 2 cut(s) 99, 155
TaiI ACGT 1 cut(s) 84
TaqI TCGA 2 cut(s) 174, 237
TauI GCSGC 2 cut(s) 8, 248
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 25
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.