Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003833

Physical Location & Seq

Genomic Coordinates Forward (+)

18705650 .. 18711808

Gene Structure 6159 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1161000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGGCTGCGGTGGCTCCAAGGTCGACAACTTGCCGCTAGTCATGCTTTGCAAGGAGCGAAAGGACCATATCAAAACGGCGTCGGACCAGCGCTACGCTCTCGCCGCAGCCCACTTCTCCTATTTCCACTCCCTCCAGCACATCGGCGACGCCCTCTGCAAGTTCGTCGACGAAGACCTCATTGTATCCGGCGCCGGCGAGTCGTCCTCGCCGCCGGGATCTCCGGTTCTCACGTTGCCTTCCGACGAAGGCAAGCCCAGAAAGAGGAAACCCCATACAAACCACTCGTCGTCTACATCGATATCGCACTCTGTTTCCGCTGATTCGCTAAAAGCGGGCGACATTGACGACGCTCACATACATTTGTCGTCTGGGTCCGAGTCAGAATCCGAGTTTGACTCATCGTCGGGTCACATTAACATCGAGGACAGCCCGGAGCAAGAGGTACCCTCTTCTTCTTCCTATCGTTACCCTCCGAATTCACATGTCTATTATATGAGAAGGACGGAGACGCCGATGCAGACGGTGTTTTATGAAGAGCCGGAGAGATATCCGACCCAAAATGGTCCTTATCCGGATCCCTATCCGCGTTATTCGGGTTTTCAGCCGTATGGCGGTGGTGGGTTTTTGGGGTACCCGATGGGGTCACCGGCGAATAGTGAGAATCCGTACAATCGACGGCAGCCAAGCCCACCACCACCAGCCGGTCCTCCACCTGCACCGCCTTCGCCTCCAAGGGTCTCGACTTGGGATTTCCTGAATGTGTTTGATACTTTTGATAATAGTGGTTACCCAGGTTACTATCCGAAGGCTAGATACGGGTACGGGTCCACGACGAGCAGTCCGGACTCGAAGGAGGTGAGGGAGAGAGAGGGGATTCCCGATTTGGAGGATGAGACAGAGCAGGAAGTGTTGAAAGAGGTTCACAAGGAGAAGCGGAGGGGGAATGCGGATGGTAATGTGAACAGGAATAGGAATCAAAATTCGGGTGAAGGGACTTCAAGGGCTGTGCCATTGAAGCAGCCAAGCAGTGAGGAGAGTTCAGGGACAGTGCCATTGCATAGCAGTGAGAGTTCCCATTCTGTCCATGGGAAGGAGATAAAGAGCAGTCCAGATACAAATGGATCGAAAAACTCCGAAGAGGAGTATGTGAAGAAAAAGAGGGTGAGTTTTGAGTTTGAGGTTGAGATTGAAGCTGCGTCGACGCATGATGTTGGATCTTCAAAGGGGAGTAGCTTAACTACATTGTCAGTCCACAGTACAAGGGACCTTCAGGAGGTTGTGAAGGAAATCAGGGATGAGTTCGAGACAGCCTCTAGTTATGGGAAAGAAGTTGCCATGCTGCTTGAGGTGGGCAAGTTGCCTTATCAGCGTAGAGGTGCTGCACTTAAAGTTGTCTTCTCGAGGATCCTTTACCTTGTAGCACATTCCCTGCTTTCTTCACCCCCGTCATCCATGCCCCCAGTAAGATTAAACTCTAAGACCGTGAAAATGGCAAACGCTTACCAGGGAGAACCTGGAAAAGATTTCAATAAGAAGCCTGGAAACCTCTCATCAACTTTGGAGAAACTATATGCATGGGAGAAAAAATTGTACAAGGAAGTCAAGGACGAAGAAAAGCTACGGGTTGACTATGAAAAGAAATGCAAGAAATTGAAAAAACTAGATTATCATGGAGCTGAGTCTGCTAAGGTTGATGCCACCCAGGCTTCTGTACGGAAGTTGCTAACAAAAATTAATGTTTGTATCAGAGCTGTTGATACCATATCGAGGAGGATACATAAACTGAGGGATGAAGAATTGCTTCCCCAAGTTGCAGAATTGATTCATGGATTGATAAGAATGTGGAAATCCATGCTCAAATGCCACCAGAAACAGTTCCAAGCTATTATGGTGAGCAAAATTCGATCTCTTAAGGTGAGTGCTGGCCTTCGAAGAGATTCTGATTTGAAAGCTACTCTTGAACTTGAAATGGAGCTTCTGAGCTGGTGCGCGTCCTTTAACAATTGGGTTAACACCCAAAAGTCCTATGTGGAGTCCTTAAATGGATGGCTTTCAAGGTGCATCAACCAGGAACCTGAAGAAACGCCTGATGGAGTTGCCCCTTTCTCCCCAAGCCAGATGGGAGCTCCACCCATTTTTGTAGTTTGTAATGATTGGTGCCAAGCAACGGAAAGAATTTCTGAAAAGGGGGTGGCAGATGCGATGCATGATTTTGCTTCAACCTTACACCAGTTATGGGAAAGGCAGGACAAGGAGCAACGTCGAAGGATCAAAGCTGAGCATGTCTCCAAGAATTTGGAGAGCCAACTTAGAAAATTGCATATGGAGAGGTTGAAAAGAGACCATGACCATGATGCATCAACAGACAAAACCGCTCTGTCAAAGTCACCTTCCGATAGTGGAGTTTCACCACTGGATGATCTGAAGGTGGATTTGGATTCAATGAAGAAGAGATTAGCCGAGGAGAGAGCGAGGCATAAGGAGGCCATCAAATTGGTTAATCATGCAGCTTCCAATAGTTTGCAAGGTGGTTTGGTCCCAATTCTTGAAACTTTAAGTAGTTTCACTTCAGAGGCTCTGAAAGGTCACGAGCAAGTCAGACTTGAAGATGCTGGAGGCTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF630 DUF632

Protein Analysis

876

Amino Acids

97.49

Weight (kDa)

6.7

Isoelectric Point (pI)

54.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF630	PF04783	1 - 59	9e-24	Protein of unknown function (DUF630)
DUF632	PF04782	424 - 744	4e-108	Protein of unknown function (DUF632)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21740 AT1G77500
fragaria_vesca	FvH4_2g27210 FvH4_2g27210 FvH4_2g27210 FvH4_2g27210 FvH4_2g27210
malus_domestica	MD08G1161000.v1.1 MD15G1346100.v1.1
prunus_persica	Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1 Prupe.1G494000_v2.0.a1
pyrus_communis	pycom08g13820 pycom15g31020
rosa_chinensis	RchiOBHm_Chr6g0296121
rosa_laevigata	RLG00000011728
rosa_multiflora	Rmu_sc0003139.1_g000046
rosa_roxburghii	Rroxscaffold_1G00036630 Rroxscaffold_7G00171810 Rroxscaffold_7G00178240
rosa_rugosa	Rorug06G0257900 Rorug06G0258000 Rorug06G0258100 Rorug06G0258200
rosa_samantha	Rh6AG370700 Rh6BG378300 Rh6CG383900 Rh6DG370700
rosa_wichuraiana	Rw6G032310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	724
Acc36I	ACCTGC	1 cut(s)	724
Acc65I	GGTACC	2 cut(s)	445, 633
AccB1I	GGYRCC	4 cut(s)	191, 445, 633, 2160
AccBSI	CCGCTC	1 cut(s)	2378
AccI	GTMKAC	4 cut(s)	24, 168, 293, 1202
AccII	CGCG	2 cut(s)	589, 1994
AccIII	TCCGGA	2 cut(s)	574, 844
AclWI	GGATC	8 cut(s)	226, 572, 585, 1132, 1225, 1402, 1415, 2279
AcsI	RAATTY	5 cut(s)	478, 982, 1902, 2178, 2296
AcuI	CTGAAG	4 cut(s)	1256, 2100, 2447, 2556
AcyI	GRCGYC	4 cut(s)	80, 150, 192, 512
AfaI	GTAC	7 cut(s)	447, 635, 671, 824, 1261, 1595, 1716
AfeI	AGCGCT	1 cut(s)	92
AfiI	CCNNNNNNNGG	8 cut(s)	264, 614, 704, 1093, 1276, 1770, 2170, 2239
AflII	CTTAAG	1 cut(s)	1913
AflIII	ACRYGT	1 cut(s)	484
AhdI	GACNNNNNGTC	2 cut(s)	403, 840
AjnI	CCWGG	6 cut(s)	793, 1506, 1516, 1540, 1704, 2070
AjuI	GAANNNNNNNTTGG	2 cut(s)	2420, 2452
AloI	GAACNNNNNNTCC	2 cut(s)	210, 242
Alw21I	GWGCWC	1 cut(s)	2131
Alw26I	GTCTC	6 cut(s)	503, 745, 890, 1301, 2293, 2337
AlwI	GGATC	8 cut(s)	226, 572, 585, 1132, 1225, 1402, 1415, 2279
Ama87I	CYCGRG	1 cut(s)	1402
Aor13HI	TCCGGA	2 cut(s)	574, 844
Aor51HI	AGCGCT	1 cut(s)	92
AoxI	GGCC	2 cut(s)	1927, 2487
ApeKI	GCWGC	8 cut(s)	6, 107, 682, 1021, 1196, 1342, 1382, 2510
ApoI	RAATTY	5 cut(s)	478, 982, 1902, 2178, 2296
ArsI	GACNNNNNNTTYG	2 cut(s)	272, 304
AseI	ATTAAT	1 cut(s)	1737
Asp700I	GAANNNNTTC	4 cut(s)	1803, 1824, 1877, 1939
Asp718I	GGTACC	2 cut(s)	445, 633
AspLEI	GCGC	3 cut(s)	93, 194, 1994
AspS9I	GGNCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
AsuC2I	CCSGG	2 cut(s)	216, 434
AsuHPI	GGTGA	9 cut(s)	639, 871, 1001, 1177, 1434, 1906, 1930, 2382, 2403
AsuII	TTCGAA	1 cut(s)	1933
AvaI	CYCGRG	1 cut(s)	1402
AvaII	GGWCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
BamHI	GGATCC	2 cut(s)	577, 1407
BanI	GGYRCC	4 cut(s)	191, 445, 633, 2160
BanII	GRGCYC	1 cut(s)	2131
BarI	GAAGNNNNNNTAC	6 cut(s)	800, 832, 1605, 1637, 2554, 2586
BauI	CACGAG	1 cut(s)	2591
BbsI	GAAGAC	2 cut(s)	180, 1390
Bbv12I	GWGCWC	1 cut(s)	2131
BbvI	GCAGC	7 cut(s)	119, 694, 1033, 1183, 1329, 1369, 2522
BccI	CCATC	6 cut(s)	634, 947, 2043, 2087, 2116, 2498
BceAI	ACGGC	3 cut(s)	93, 592, 695
BcgI	CGANNNNNNTGC	4 cut(s)	148, 182, 708, 742
BciT130I	CCWGG	6 cut(s)	795, 1508, 1518, 1542, 1706, 2072
BciVI	GTATCC	2 cut(s)	196, 1770
BcnI	CCSGG	2 cut(s)	216, 434
BcoDI	GTCTC	6 cut(s)	503, 745, 890, 1301, 2293, 2337
BfaI	CTAG	4 cut(s)	38, 813, 1317, 1664
BfoI	RGCGCY	2 cut(s)	94, 195
BfrI	CTTAAG	1 cut(s)	1913
BfuAI	ACCTGC	1 cut(s)	724
BfuI	GTATCC	2 cut(s)	196, 1770
BglI	GCCNNNNNGGC	1 cut(s)	1706
BlpI	GCTNAGC	1 cut(s)	2280
Bme1390I	CCNGG	8 cut(s)	216, 434, 795, 1508, 1518, 1542, 1706, 2072
Bme18I	GGWCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
BmeRI	GACNNNNNGTC	2 cut(s)	403, 840
BmeT110I	CYCGRG	1 cut(s)	1402
BmgT120I	GGNCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
BmrFI	CCNGG	8 cut(s)	216, 434, 795, 1508, 1518, 1542, 1706, 2072
BmrI	ACTGGG	1 cut(s)	1457
BmsI	GCATC	8 cut(s)	507, 1687, 2073, 2191, 2196, 2347, 2369, 2602
BmuI	ACTGGG	1 cut(s)	1457
BpiI	GAAGAC	2 cut(s)	180, 1390
BpmI	CTGGAG	1 cut(s)	119
Bpu10I	CCTNAGC	1 cut(s)	1689
Bpu1102I	GCTNAGC	1 cut(s)	2280
Bpu14I	TTCGAA	1 cut(s)	1933
BpuEI	CTTGAG	1 cut(s)	1367
BpuMI	CCSGG	2 cut(s)	216, 434
Bsa29I	ATCGAT	1 cut(s)	299
BsaBI	GATNNNNATC	1 cut(s)	582
BsaHI	GRCGYC	4 cut(s)	80, 150, 192, 512
BsaI	GGTCTC	2 cut(s)	745, 2337
BsaJI	CCNNGG	7 cut(s)	18, 734, 793, 1087, 1507, 1704, 2463
BsaWI	WCCGGW	3 cut(s)	223, 574, 844
Bsc4I	CCNNNNNNNGG	8 cut(s)	264, 614, 704, 1093, 1276, 1770, 2170, 2239
Bse118I	RCCGGY	3 cut(s)	194, 649, 704
Bse1I	ACTGG	3 cut(s)	1463, 2233, 2421
Bse3DI	GCAATG	1 cut(s)	1055
Bse8I	GATNNNNATC	1 cut(s)	582
BseAI	TCCGGA	2 cut(s)	574, 844
BseBI	CCWGG	6 cut(s)	795, 1508, 1518, 1542, 1706, 2072
BseCI	ATCGAT	1 cut(s)	299
BseDI	CCNNGG	7 cut(s)	18, 734, 793, 1087, 1507, 1704, 2463
BseGI	GGATG	7 cut(s)	898, 958, 1303, 1451, 1798, 2054, 2425
BseJI	GATNNNNATC	1 cut(s)	582
BseLI	CCNNNNNNNGG	8 cut(s)	264, 614, 704, 1093, 1276, 1770, 2170, 2239
BseMI	GCAATG	1 cut(s)	1055
BseMII	CTCAG	4 cut(s)	1671, 1778, 1973, 2271
BseNI	ACTGG	3 cut(s)	1463, 2233, 2421
BseRI	GAGGAG	4 cut(s)	1049, 1157, 1786, 2480
BseXI	GCAGC	7 cut(s)	119, 694, 1033, 1183, 1329, 1369, 2522
BsgI	GTGCAG	2 cut(s)	702, 1368
Bsh1236I	CGCG	2 cut(s)	589, 1994
BshFI	GGCC	2 cut(s)	1929, 2489
BshNI	GGYRCC	4 cut(s)	191, 445, 633, 2160
BshVI	ATCGAT	1 cut(s)	299
BsiHKAI	GWGCWC	1 cut(s)	2131
BsiHKCI	CYCGRG	1 cut(s)	1402
BslFI	GGGAC	4 cut(s)	1009, 1060, 1280, 2525
BslI	CCNNNNNNNGG	8 cut(s)	264, 614, 704, 1093, 1276, 1770, 2170, 2239
BsmAI	GTCTC	6 cut(s)	503, 745, 890, 1301, 2293, 2337
BsmBI	CGTCTC	1 cut(s)	503
BsmFI	GGGAC	4 cut(s)	1009, 1060, 1280, 2525
BsmI	GAATGC	1 cut(s)	952
BsnI	GGCC	2 cut(s)	1929, 2489
Bso31I	GGTCTC	2 cut(s)	745, 2337
BsoBI	CYCGRG	1 cut(s)	1402
Bsp119I	TTCGAA	1 cut(s)	1933
Bsp1286I	GDGCHC	1 cut(s)	2131
Bsp13I	TCCGGA	2 cut(s)	574, 844
Bsp1407I	TGTACA	1 cut(s)	1593
Bsp143I	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
Bsp1720I	GCTNAGC	1 cut(s)	2280
Bsp19I	CCATGG	1 cut(s)	1087
BspANI	GGCC	2 cut(s)	1929, 2489
BspCNI	CTCAG	4 cut(s)	1672, 1779, 1974, 2272
BspDI	ATCGAT	1 cut(s)	299
BspEI	TCCGGA	2 cut(s)	574, 844
BspFNI	CGCG	2 cut(s)	589, 1994
BspMI	ACCTGC	1 cut(s)	724
BspPI	GGATC	8 cut(s)	226, 572, 585, 1132, 1225, 1402, 1415, 2279
BspQI	GCTCTTC	1 cut(s)	531
BspT104I	TTCGAA	1 cut(s)	1933
BspT107I	GGYRCC	4 cut(s)	191, 445, 633, 2160
BspTI	CTTAAG	1 cut(s)	1913
BspTNI	GGTCTC	2 cut(s)	745, 2337
BsrBI	CCGCTC	1 cut(s)	2378
BsrDI	GCAATG	1 cut(s)	1055
BsrFI	RCCGGY	3 cut(s)	194, 649, 704
BsrGI	TGTACA	1 cut(s)	1593
BsrI	ACTGG	3 cut(s)	1463, 2233, 2421
BssAI	RCCGGY	3 cut(s)	194, 649, 704
BssECI	CCNNGG	7 cut(s)	18, 734, 793, 1087, 1507, 1704, 2463
BssMI	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
BssNI	GRCGYC	4 cut(s)	80, 150, 192, 512
BssSI	CACGAG	1 cut(s)	2591
BssT1I	CCWWGG	3 cut(s)	18, 734, 1087
Bst2BI	CACGAG	1 cut(s)	2591
Bst2UI	CCWGG	6 cut(s)	795, 1508, 1518, 1542, 1706, 2072
Bst4CI	ACNGT	5 cut(s)	526, 1051, 1259, 1486, 1878
Bst6I	CTCTTC	5 cut(s)	457, 531, 1134, 1930, 2447
BstACI	GRCGYC	4 cut(s)	80, 150, 192, 512
BstAFI	CTTAAG	1 cut(s)	1913
BstAUI	TGTACA	1 cut(s)	1593
BstBI	TTCGAA	1 cut(s)	1933
BstC8I	GCNNGC	4 cut(s)	196, 254, 337, 1927
BstDEI	CTNAG	7 cut(s)	1479, 1680, 1689, 1787, 1982, 2280, 2312
BstDSI	CCRYGG	1 cut(s)	1087
BstEII	GGTNACC	2 cut(s)	645, 788
BstENI	CCTNNNNNAGG	1 cut(s)	1274
BstF5I	GGATG	7 cut(s)	898, 958, 1303, 1451, 1798, 2054, 2425
BstFNI	CGCG	2 cut(s)	589, 1994
BstH2I	RGCGCY	2 cut(s)	94, 195
BstHHI	GCGC	3 cut(s)	93, 194, 1994
BstKTI	GATC	8 cut(s)	221, 580, 1127, 1220, 1410, 1910, 2274, 2425
BstMAI	GTCTC	6 cut(s)	503, 745, 890, 1301, 2293, 2337
BstMBI	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1018, 1369, 1685, 1706
BstNI	CCWGG	6 cut(s)	795, 1508, 1518, 1542, 1706, 2072
BstNSI	RCATGY	2 cut(s)	488, 2288
BstPI	GGTNACC	2 cut(s)	645, 788
BstSCI	CCNGG	8 cut(s)	214, 432, 793, 1506, 1516, 1540, 1704, 2070
BstUI	CGCG	2 cut(s)	589, 1994
BstV1I	GCAGC	7 cut(s)	119, 694, 1033, 1183, 1329, 1369, 2522
BstV2I	GAAGAC	2 cut(s)	180, 1390
BstX2I	RGATCY	4 cut(s)	218, 577, 1217, 1407
BstXI	CCANNNNNNTGG	2 cut(s)	2299, 2497
BstYI	RGATCY	4 cut(s)	218, 577, 1217, 1407
Bsu15I	ATCGAT	1 cut(s)	299
BsuI	GTATCC	2 cut(s)	196, 1770
BsuRI	GGCC	2 cut(s)	1929, 2489
BsuTUI	ATCGAT	1 cut(s)	299
BtgI	CCRYGG	1 cut(s)	1087
BtgZI	GCGATG	1 cut(s)	2219
BtsCI	GGATG	7 cut(s)	898, 958, 1303, 1451, 1798, 2054, 2425
BtsI	GCAGTG	2 cut(s)	1036, 1072
BtsIMutI	CAGTG	4 cut(s)	1036, 1056, 1072, 2414
BveI	ACCTGC	1 cut(s)	724
Cac8I	GCNNGC	4 cut(s)	196, 254, 337, 1927
CfoI	GCGC	3 cut(s)	93, 194, 1994
Cfr10I	RCCGGY	3 cut(s)	194, 649, 704
Cfr13I	GGNCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
ClaI	ATCGAT	1 cut(s)	299
CseI	GACGC	7 cut(s)	69, 158, 359, 520, 1188, 1213, 1983
Csp6I	GTAC	7 cut(s)	446, 634, 670, 823, 1260, 1594, 1715
CviQI	GTAC	7 cut(s)	446, 634, 670, 823, 1260, 1594, 1715
DdeI	CTNAG	7 cut(s)	1479, 1680, 1689, 1787, 1982, 2280, 2312
DinI	GGCGCC	1 cut(s)	193
DpnI	GATC	8 cut(s)	220, 579, 1126, 1219, 1409, 1909, 2273, 2424
DpnII	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
DriI	GACNNNNNGTC	2 cut(s)	403, 840
Eam1104I	CTCTTC	5 cut(s)	457, 531, 1134, 1930, 2447
Eam1105I	GACNNNNNGTC	2 cut(s)	403, 840
EarI	CTCTTC	5 cut(s)	457, 531, 1134, 1930, 2447
Ecl136II	GAGCTC	1 cut(s)	2129
Eco130I	CCWWGG	3 cut(s)	18, 734, 1087
Eco24I	GRGCYC	1 cut(s)	2131
Eco31I	GGTCTC	2 cut(s)	745, 2337
Eco32I	GATATC	2 cut(s)	303, 551
Eco47I	GGWCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
Eco47III	AGCGCT	1 cut(s)	92
Eco53kI	GAGCTC	1 cut(s)	2129
Eco57I	CTGAAG	4 cut(s)	1256, 2100, 2447, 2556
Eco88I	CYCGRG	1 cut(s)	1402
Eco91I	GGTNACC	2 cut(s)	645, 788
EcoICRI	GAGCTC	1 cut(s)	2129
EcoNI	CCTNNNNNAGG	1 cut(s)	1274
EcoO109I	RGGNCCY	1 cut(s)	1267
EcoO65I	GGTNACC	2 cut(s)	645, 788
EcoRI	GAATTC	1 cut(s)	478
EcoRII	CCWGG	6 cut(s)	793, 1506, 1516, 1540, 1704, 2070
EcoRV	GATATC	2 cut(s)	303, 551
EcoT14I	CCWWGG	3 cut(s)	18, 734, 1087
EcoT22I	ATGCAT	3 cut(s)	1579, 2211, 2362
EcoT38I	GRGCYC	1 cut(s)	2131
EgeI	GGCGCC	1 cut(s)	193
EheI	GGCGCC	1 cut(s)	193
ErhI	CCWWGG	3 cut(s)	18, 734, 1087
Esp3I	CGTCTC	1 cut(s)	503
FalI	AAGNNNNNCTT	6 cut(s)	1788, 1820, 1944, 1976, 2589, 2621
FaqI	GGGAC	4 cut(s)	1009, 1060, 1280, 2525
FauI	CCCGC	1 cut(s)	328
FauNDI	CATATG	1 cut(s)	2325
FblI	GTMKAC	4 cut(s)	24, 168, 293, 1202
FokI	GGATG	7 cut(s)	905, 965, 1310, 1438, 1805, 2061, 2432
FriOI	GRGCYC	1 cut(s)	2131
FspBI	CTAG	4 cut(s)	38, 813, 1317, 1664
GlaI	GCGC	3 cut(s)	92, 193, 1993
GsuI	CTGGAG	1 cut(s)	119
HaeII	RGCGCY	2 cut(s)	94, 195
HaeIII	GGCC	2 cut(s)	1929, 2489
HgaI	GACGC	7 cut(s)	69, 158, 359, 520, 1188, 1213, 1983
HhaI	GCGC	3 cut(s)	93, 194, 1994
Hin1I	GRCGYC	4 cut(s)	80, 150, 192, 512
Hin6I	GCGC	3 cut(s)	91, 192, 1992
HinP1I	GCGC	3 cut(s)	91, 192, 1992
HincII	GTYRAC	5 cut(s)	25, 169, 1203, 1630, 2014
HindII	GTYRAC	5 cut(s)	25, 169, 1203, 1630, 2014
HpaI	GTTAAC	1 cut(s)	2014
HphI	GGTGA	9 cut(s)	639, 871, 1001, 1177, 1434, 1906, 1930, 2382, 2403
HpyCH4III	ACNGT	5 cut(s)	526, 1051, 1259, 1486, 1878
HpyCH4IV	ACGT	2 cut(s)	233, 2263
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1018, 1369, 1685, 1706
HpyF3I	CTNAG	7 cut(s)	1479, 1680, 1689, 1787, 1982, 2280, 2312
HpySE526I	ACGT	2 cut(s)	233, 2263
Hsp92I	GRCGYC	4 cut(s)	80, 150, 192, 512
HspAI	GCGC	3 cut(s)	91, 192, 1992
KasI	GGCGCC	1 cut(s)	191
Kpn2I	TCCGGA	2 cut(s)	574, 844
KpnI	GGTACC	2 cut(s)	449, 637
KroI	GCCGGC	1 cut(s)	194
KroNI	GCCGGC	1 cut(s)	196
KspAI	GTTAAC	1 cut(s)	2014
Kzo9I	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
LguI	GCTCTTC	1 cut(s)	531
LmnI	GCTCC	8 cut(s)	20, 55, 436, 1676, 1975, 2126, 2134, 2257
Lsp1109I	GCAGC	7 cut(s)	119, 694, 1033, 1183, 1329, 1369, 2522
LweI	GCATC	8 cut(s)	507, 1687, 2073, 2191, 2196, 2347, 2369, 2602
MaeI	CTAG	4 cut(s)	38, 813, 1317, 1664
MaeII	ACGT	2 cut(s)	233, 2263
MaeIII	GTNAC	7 cut(s)	410, 467, 645, 788, 797, 2388, 2588
MalI	GATC	8 cut(s)	220, 579, 1126, 1219, 1409, 1909, 2273, 2424
MbiI	CCGCTC	1 cut(s)	2378
MboI	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
MfeI	CAATTG	1 cut(s)	2005
MflI	RGATCY	4 cut(s)	218, 577, 1217, 1407
MhlI	GDGCHC	1 cut(s)	2131
Mly113I	GGCGCC	1 cut(s)	192
MlyI	GAGTC	6 cut(s)	209, 389, 392, 842, 1691, 2045
MmeI	TCCRAC	4 cut(s)	63, 267, 578, 1195
Mph1103I	ATGCAT	3 cut(s)	1579, 2211, 2362
MreI	CGCCGGCG	1 cut(s)	194
MroI	TCCGGA	2 cut(s)	574, 844
MroNI	GCCGGC	1 cut(s)	194
MroXI	GAANNNNTTC	4 cut(s)	1803, 1824, 1877, 1939
MslI	CAYNNNNRTG	2 cut(s)	1065, 2352
MspA1I	CMGCKG	1 cut(s)	320
MspCI	CTTAAG	1 cut(s)	1913
MspR9I	CCNGG	8 cut(s)	216, 434, 795, 1508, 1518, 1542, 1706, 2072
MunI	CAATTG	1 cut(s)	2005
Mva1269I	GAATGC	1 cut(s)	952
MvaI	CCWGG	6 cut(s)	795, 1508, 1518, 1542, 1706, 2072
MvnI	CGCG	2 cut(s)	589, 1994
MwoI	GCNNNNNNNGC	7 cut(s)	12, 43, 104, 1018, 1369, 1685, 1706
NaeI	GCCGGC	1 cut(s)	196
NarI	GGCGCC	1 cut(s)	192
NciI	CCSGG	2 cut(s)	216, 434
NcoI	CCATGG	1 cut(s)	1087
NdeI	CATATG	1 cut(s)	2325
NdeII	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
NgoMIV	GCCGGC	1 cut(s)	194
NmeAIII	GCCGAG	1 cut(s)	2488
NmuCI	GTSAC	4 cut(s)	410, 645, 2388, 2588
NsiI	ATGCAT	3 cut(s)	1579, 2211, 2362
NspI	RCATGY	2 cut(s)	488, 2288
NspV	TTCGAA	1 cut(s)	1933
PaeR7I	CTCGAG	1 cut(s)	1402
PaqCI	CACCTGC	1 cut(s)	724
PciI	ACATGT	1 cut(s)	484
PciSI	GCTCTTC	1 cut(s)	531
PcsI	WCGNNNNNNNCGW	2 cut(s)	296, 512
PctI	GAATGC	1 cut(s)	952
PdiI	GCCGGC	1 cut(s)	196
PdmI	GAANNNNTTC	4 cut(s)	1803, 1824, 1877, 1939
PfeI	GAWTC	8 cut(s)	323, 386, 664, 877, 976, 1825, 1940, 2441
PleI	GAGTC	6 cut(s)	208, 388, 392, 842, 1690, 2044
PluTI	GGCGCC	1 cut(s)	195
PpsI	GAGTC	6 cut(s)	208, 388, 392, 842, 1690, 2044
PpuMI	RGGWCCY	1 cut(s)	1267
PscI	ACATGT	1 cut(s)	484
PshBI	ATTAAT	1 cut(s)	1737
Psp124BI	GAGCTC	1 cut(s)	2131
Psp5II	RGGWCCY	1 cut(s)	1267
Psp6I	CCWGG	6 cut(s)	793, 1506, 1516, 1540, 1704, 2070
PspEI	GGTNACC	2 cut(s)	645, 788
PspGI	CCWGG	6 cut(s)	793, 1506, 1516, 1540, 1704, 2070
PspPI	GGNCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
PspPPI	RGGWCCY	1 cut(s)	1267
PsuI	RGATCY	4 cut(s)	218, 577, 1217, 1407
RsaI	GTAC	7 cut(s)	447, 635, 671, 824, 1261, 1595, 1716
RsaNI	GTAC	7 cut(s)	446, 634, 670, 823, 1260, 1594, 1715
RseI	CAYNNNNRTG	2 cut(s)	1065, 2352
SacI	GAGCTC	1 cut(s)	2131
SalI	GTCGAC	3 cut(s)	23, 167, 1201
SapI	GCTCTTC	1 cut(s)	531
Sau3AI	GATC	8 cut(s)	218, 577, 1124, 1217, 1407, 1907, 2271, 2422
Sau96I	GGNCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
SchI	GAGTC	6 cut(s)	209, 389, 392, 842, 1691, 2045
ScrFI	CCNGG	8 cut(s)	216, 434, 795, 1508, 1518, 1542, 1706, 2072
SduI	GDGCHC	1 cut(s)	2131
SfaNI	GCATC	8 cut(s)	507, 1687, 2073, 2191, 2196, 2347, 2369, 2602
SfoI	GGCGCC	1 cut(s)	193
Sfr274I	CTCGAG	1 cut(s)	1402
SfuI	TTCGAA	1 cut(s)	1933
SgrAI	CRCCGGYG	2 cut(s)	194, 649
SgrDI	CGTCGACG	2 cut(s)	167, 1201
SinI	GGWCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
SlaI	CTCGAG	1 cut(s)	1402
SmiMI	CAYNNNNRTG	2 cut(s)	1065, 2352
SmlI	CTYRAG	3 cut(s)	1346, 1402, 1913
SmoI	CTYRAG	3 cut(s)	1346, 1402, 1913
SspDI	GGCGCC	1 cut(s)	191
SspMI	CTAG	4 cut(s)	38, 813, 1317, 1664
SstI	GAGCTC	1 cut(s)	2131
StyD4I	CCNGG	8 cut(s)	214, 432, 793, 1506, 1516, 1540, 1704, 2070
StyI	CCWWGG	3 cut(s)	18, 734, 1087
TaaI	ACNGT	5 cut(s)	526, 1051, 1259, 1486, 1878
TaiI	ACGT	2 cut(s)	236, 2266
TatI	WGTACW	2 cut(s)	1259, 1593
TauI	GCSGC	3 cut(s)	37, 107, 214
TfiI	GAWTC	8 cut(s)	323, 386, 664, 877, 976, 1825, 1940, 2441
TscAI	CASTG	4 cut(s)	1036, 1056, 1072, 2421
TseFI	GTSAC	4 cut(s)	410, 645, 2388, 2588
TseI	GCWGC	8 cut(s)	6, 107, 682, 1021, 1196, 1342, 1382, 2510
Tsp45I	GTSAC	4 cut(s)	410, 645, 2388, 2588
TspDTI	ATGAA	5 cut(s)	549, 1650, 1809, 1817, 2462
TspGWI	ACGGA	4 cut(s)	521, 657, 1732, 2186
TspRI	CASTG	4 cut(s)	1036, 1056, 1072, 2421
Vha464I	CTTAAG	1 cut(s)	1913
VpaK11BI	GGWCC	8 cut(s)	64, 85, 375, 566, 707, 828, 1267, 2539
VspI	ATTAAT	1 cut(s)	1737
XagI	CCTNNNNNAGG	1 cut(s)	1274
XapI	RAATTY	5 cut(s)	478, 982, 1902, 2178, 2296
XceI	RCATGY	2 cut(s)	488, 2288
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1085, 1514
XhoI	CTCGAG	1 cut(s)	1402
XmiI	GTMKAC	4 cut(s)	24, 168, 293, 1202
XmnI	GAANNNNTTC	4 cut(s)	1803, 1824, 1877, 1939
XspI	CTAG	4 cut(s)	38, 813, 1317, 1664
Zsp2I	ATGCAT	3 cut(s)	1579, 2211, 2362

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.