Rorug06G0258100

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003833
Physical Location & Seq
Forward (+)
40530747 .. 40538029
7283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0258100.1

Sequence Viewer

Length: 894 bp
ATGGTGAGAGTCAGCGTTTTGAACGATGCTCGGAAGAGCATGTACAACGCAGAGAAGAGGGGCAAGAGACAGTTCATGATCAGACCTTCGTCCAAGGTCATAATCAAGTTCCTTATCGTTATGCAGAAGCACGGTTATATTGGAGAGTTTGAGTATGTTGATGACCACAGATCTGGAAAGATTGTGGTTGAACTCAATGGGAGGCTGAACAAGTGTGGGGTTATCAGTCCTCGCTTTGATGTTGGTGTCAAGGAGATTGAAGGCTGGACGGCTAGACTTCTTCCGTCAAGACAGGTTTGTTCTCTGTGTAGGGCTTTGTCATGGTTTTTTGTTGGCTGTTACTACTATTGTATAAAAAAGTATGATCAGTCACGCCGTTATTTCAGTAAGGCTACCAATGTCGATGGAAACTTTCCACCTGCATGGATAGGATATGGGAATGCTTATTCTGCTCAAGATGAGGGAGATCAAGCTATGTCAATTTATCGCACTGCTGCTCGTTTGTTTCCAGGGTGTCATTTACCAACTTTGTACATTGGAATGGAGTACATGCGAACCCACAGCTACAAACTTGCTGAGCAGGGTATTCTTAATGATGGAAGAGTAATTGCCATGAAGCAATTTACAGTGACTAAGAAGAAGTTGAGGCAGAGAATTTGGAACCTGCTAAGTACATTATGTATTCAAATGGTTTTTGATTTGGTCGACCCACAAATAGAAGTCCTGAAACACTGCCAAGTGTTAAATAAGGATTGGGGTTTAATGCAAACTGCAGATAAGAATCAGTGGTTTCAGAATCTCAGATATACAAGTGCTGCAGTGTTGGCATGGCTCATCTTTGTTTTTAAACAATCTTTGGCTGGTCTTAGAATTGTGATTGTTGGTTGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

297

Amino Acids

34.4

Weight (kDa)

9.66

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 7 - 88 1.8e-08 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 2 cut(s) 427, 672
AccI GTMKAC 1 cut(s) 705
AcsI RAATTY 1 cut(s) 654
AfaI GTAC 4 cut(s) 44, 533, 548, 673
AgsI TTSAA 4 cut(s) 22, 191, 260, 686
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 2 cut(s) 473, 564
AluI AGCT 2 cut(s) 473, 564
Alw26I GTCTC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 494, 815
ApoI RAATTY 1 cut(s) 654
AsuHPI GGTGA 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 26, 58
BbvI GCAGC 2 cut(s) 481, 802
BccI CCATC 2 cut(s) 398, 590
BceAI ACGGC 2 cut(s) 285, 360
BciT130I CCWGG 1 cut(s) 510
BclI TGATCA 2 cut(s) 78, 364
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 2 cut(s) 771, 816
BfuAI ACCTGC 2 cut(s) 427, 672
BglII AGATCT 1 cut(s) 170
BisI GCNGC 2 cut(s) 495, 816
BlpI GCTNAGC 1 cut(s) 576
BlsI GCNGC 2 cut(s) 496, 817
Bme1390I CCNGG 1 cut(s) 510
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 510
BmsI GCATC 1 cut(s) 16
BoxI GACNNNNGTC 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 438
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 2 cut(s) 93, 509
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 93, 509
BseJI GATNNNNATC 1 cut(s) 780
BseMII CTCAG 2 cut(s) 567, 814
BseXI GCAGC 2 cut(s) 481, 802
BsmAI GTCTC 1 cut(s) 61
BsmI GAATGC 1 cut(s) 445
Bsp1407I TGTACA 2 cut(s) 42, 531
Bsp143I GATC 4 cut(s) 78, 170, 364, 466
Bsp1720I GCTNAGC 1 cut(s) 576
BspCNI CTCAG 2 cut(s) 568, 813
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 662
BspMAI CTGCAG 2 cut(s) 775, 820
BspMI ACCTGC 2 cut(s) 427, 672
BspQI GCTCTTC 1 cut(s) 29
BsrGI TGTACA 2 cut(s) 42, 531
BssECI CCNNGG 2 cut(s) 93, 509
BssMI GATC 4 cut(s) 78, 170, 364, 466
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 3 cut(s) 72, 134, 628
Bst6I CTCTTC 3 cut(s) 29, 50, 595
BstAUI TGTACA 2 cut(s) 42, 531
BstDEI CTNAG 5 cut(s) 576, 633, 668, 800, 866
BstKTI GATC 4 cut(s) 81, 173, 367, 469
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 4 cut(s) 78, 170, 364, 466
BstMWI GCNNNNNNNGC 2 cut(s) 449, 824
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 2 cut(s) 43, 553
BstPAI GACNNNNGTC 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 508
BstSFI CTRYAG 2 cut(s) 771, 816
BstV1I GCAGC 2 cut(s) 481, 802
BstX2I RGATCY 1 cut(s) 170
BstXI CCANNNNNNTGG 2 cut(s) 173, 423
BstYI RGATCY 1 cut(s) 170
BtsI GCAGTG 3 cut(s) 489, 730, 825
BtsIMutI CAGTG 5 cut(s) 489, 633, 730, 791, 825
BveI ACCTGC 2 cut(s) 427, 672
CciI TCATGA 1 cut(s) 75
Csp6I GTAC 4 cut(s) 43, 532, 547, 672
CviAII CATG 7 cut(s) 40, 76, 321, 423, 550, 613, 828
CviQI GTAC 4 cut(s) 43, 532, 547, 672
DdeI CTNAG 5 cut(s) 576, 633, 668, 800, 866
DpnI GATC 4 cut(s) 80, 172, 366, 468
DpnII GATC 4 cut(s) 78, 170, 364, 466
DraI TTTAAA 1 cut(s) 847
Eam1104I CTCTTC 3 cut(s) 29, 50, 595
EarI CTCTTC 3 cut(s) 29, 50, 595
Eco130I CCWWGG 1 cut(s) 93
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 7 cut(s) 43, 79, 324, 426, 553, 616, 831
FatI CATG 7 cut(s) 39, 75, 320, 422, 549, 612, 827
FbaI TGATCA 2 cut(s) 78, 364
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 2 cut(s) 495, 816
Fsp4HI GCNGC 2 cut(s) 495, 816
FspBI CTAG 1 cut(s) 273
GluI GCNGC 2 cut(s) 495, 816
Hin1II CATG 7 cut(s) 43, 79, 324, 426, 553, 616, 831
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 3 cut(s) 9, 781, 796
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 4 cut(s) 33, 83, 795, 803
Hpy188III TCNNGA 5 cut(s) 76, 174, 288, 455, 724
Hpy8I GTNNAC 1 cut(s) 706
HpyAV CCTTC 2 cut(s) 96, 254
HpyCH4III ACNGT 3 cut(s) 72, 134, 628
HpyCH4V TGCA 5 cut(s) 124, 422, 766, 773, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 824
HpyF3I CTNAG 5 cut(s) 576, 633, 668, 800, 866
Hsp92II CATG 7 cut(s) 43, 79, 324, 426, 553, 616, 831
Ksp22I TGATCA 2 cut(s) 78, 364
Kzo9I GATC 4 cut(s) 78, 170, 364, 466
LguI GCTCTTC 1 cut(s) 29
Lsp1109I GCAGC 2 cut(s) 481, 802
LweI GCATC 1 cut(s) 16
MaeI CTAG 1 cut(s) 273
MaeIII GTNAC 3 cut(s) 338, 369, 628
MalI GATC 4 cut(s) 80, 172, 366, 468
MboI GATC 4 cut(s) 78, 170, 364, 466
MboII GAAGA 5 cut(s) 46, 67, 272, 612, 649
MflI RGATCY 1 cut(s) 170
MluCI AATT 5 cut(s) 480, 606, 620, 654, 870
MlyI GAGTC 1 cut(s) 18
MnlI CCTC 5 cut(s) 51, 195, 240, 454, 639
MseI TTAA 4 cut(s) 591, 743, 761, 846
MslI CAYNNNNRTG 2 cut(s) 421, 539
MspR9I CCNGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 445
MvaI CCWGG 1 cut(s) 510
MwoI GCNNNNNNNGC 2 cut(s) 449, 824
NdeII GATC 4 cut(s) 78, 170, 364, 466
NlaIII CATG 7 cut(s) 43, 79, 324, 426, 553, 616, 831
NlaIV GGNNCC 1 cut(s) 662
NmuCI GTSAC 2 cut(s) 369, 628
NspI RCATGY 2 cut(s) 43, 553
PagI TCATGA 1 cut(s) 75
PaqCI CACCTGC 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 29
PctI GAATGC 1 cut(s) 445
PfeI GAWTC 2 cut(s) 781, 796
PkrI GCNGC 2 cut(s) 496, 817
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
PshAI GACNNNNGTC 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 508
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 662
PstI CTGCAG 2 cut(s) 775, 820
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 4 cut(s) 44, 533, 548, 673
RsaNI GTAC 4 cut(s) 43, 532, 547, 672
RseI CAYNNNNRTG 2 cut(s) 421, 539
SalI GTCGAC 1 cut(s) 704
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 4 cut(s) 591, 743, 761, 846
SatI GCNGC 2 cut(s) 495, 816
Sau3AI GATC 4 cut(s) 78, 170, 364, 466
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 1 cut(s) 510
SetI ASST 7 cut(s) 88, 99, 297, 421, 475, 566, 666
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 2 cut(s) 771, 816
SmiMI CAYNNNNRTG 2 cut(s) 421, 539
SmlI CTYRAG 1 cut(s) 453
SmoI CTYRAG 1 cut(s) 453
Sse9I AATT 5 cut(s) 480, 606, 620, 654, 870
SspMI CTAG 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 72, 134, 628
TaqI TCGA 2 cut(s) 402, 705
TasI AATT 5 cut(s) 480, 606, 620, 654, 870
TatI WGTACW 4 cut(s) 42, 531, 546, 671
TfiI GAWTC 2 cut(s) 781, 796
Tru1I TTAA 4 cut(s) 591, 743, 761, 846
Tru9I TTAA 4 cut(s) 591, 743, 761, 846
TscAI CASTG 5 cut(s) 496, 633, 737, 791, 825
TseFI GTSAC 2 cut(s) 369, 628
TseI GCWGC 2 cut(s) 494, 815
Tsp45I GTSAC 2 cut(s) 369, 628
TspDTI ATGAA 2 cut(s) 64, 629
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 5 cut(s) 496, 633, 737, 791, 825
XapI RAATTY 1 cut(s) 654
XceI RCATGY 2 cut(s) 43, 553
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.