MD08G1180300.v1.1

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011866
Physical Location & Seq
Reverse (-)
22187187 .. 22194051
6865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1180300.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGAGAATATAATGAGCAAGCTTCGGAATTTGGATGCTTACCCAAAAATCAATGAAGATTTCTACAGTCGTACACTCTCCGGCGGCGTTATCACCCTCGCTTCCTCCATCGTCATGCTCCTGCTCTTCCTCTCCGAGCTTCGGTTATATCTCCATGCGGTAACGGAATCAAAGCTTGTGGTAGATACTTCTAGAGGCGAAACCTTACGAATCAATTTTGATGTGACTTTTGCTGCCTTGCCTTGTTCTATACTAAGTCTCGATGCCATGGACATCAGTGGCGAGCAGCACCTAGATGTAAAACATGACATTATCAAAAAAAGATTAGACTCCCATGGAAATGTGATAGAATCAAGGCCAGATGTTATTGGTGCACCCAAGATTGATAAGCCCTTACAGAGACATGGTGGCAGGCTTGAGCACAATGAGACATACTGTGGTTCATGTTATGGTGCAGAAGCGGCTGACGAGGATTGTTGTAATTCATGTGAAGATGTCCGTGAAGCTTACCGAAAGAAAGGTTGGGCAATATCAAATCCCGATTTGATTGACCAGTGCAAAAGAGAAGGCTTTCTGCAAAGAATTAAAGATGAAGAAGGGGAAGGGTGCAATATATATGGATTCTTGGAAGTCAACAAGGTAGCTGGGAACTTCCATTTTGCACCAGGGAAAAGCTTTCAACAATCAAATGTTCATGTACATGATCTGTTGGCATTTCAAAAGGATAGTTTCAATATAAGTCACACGATCAACAGATTGTCTTTTGGAGACTATTTCCCTGGTGTTGTAAATCCTCTTGATGGTGTGCACTGGACTCAAGACAACCCAAGTGGGATGTATCAATACTTTATCAAGGTTGTACCTACTGTGTATACAGACGTGAGTGGGCACACTATACAGTCAAATCAGTTCTCTGTGACCGAACATTATAGGGGTACAGAAATGGGCAACCTTCAGTTCCTTCCTGGAGTGTTTTTCTTCTATGATCTTTCTCCTATAAAGGTAACTTTCACGGAGGAGCATATTTCATTCCTGCACTTCCTGACAAATGTTTGTGCCATAGTCGGAGGTGTTTTTACAGTTTCGGGGATACTAGATTCATTTATATACCATGGCCAGAAGGCAATCAAGAAGAAGATGGAAATTGGCAAGTTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

387

Amino Acids

43.42

Weight (kDa)

5.8

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 8 - 97 2.6e-31 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 149 - 366 6.3e-89 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36050 AT1G36050
fragaria_vesca FvH4_5g37810 FvH4_5g37810 FvH4_5g37810
malus_domestica MD08G1180300.v1.1 MD15G1365600.v1.1
prunus_persica Prupe.1G513700_v2.0.a1
pyrus_communis pycom08g15430 pycom15g32800
rosa_chinensis RchiOBHm_Chr7g0239691
rosa_laevigata RLG00000000741
rosa_multiflora Rmu_sc0000063.1_g000019
rosa_roxburghii Rroxscaffold_3G00220830
rosa_samantha Rh7AG482900 Rh7BG455600 Rh7CG500300 Rh7DG468500
rosa_wichuraiana Rw7G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 872
AciI CCGC 3 cut(s) 84, 158, 461
AcoI YGGCCR 1 cut(s) 1114
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 938
AfaI GTAC 4 cut(s) 73, 699, 861, 937
AfiI CCNNNNNNNGG 2 cut(s) 141, 800
AgsI TTSAA 3 cut(s) 680, 719, 733
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 3 cut(s) 664, 778, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
AluI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
Alw21I GWGCWC 3 cut(s) 376, 423, 810
Alw26I GTCTC 4 cut(s) 263, 394, 422, 762
Alw44I GTGCAC 2 cut(s) 372, 806
AoxI GGCC 2 cut(s) 356, 1114
ApaLI GTGCAC 2 cut(s) 372, 806
ApeKI GCWGC 2 cut(s) 233, 286
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 570
AsuHPI GGTGA 1 cut(s) 85
BaeGI GKGCMC 3 cut(s) 376, 810, 891
BalI TGGCCA 1 cut(s) 1116
Bbv12I GWGCWC 3 cut(s) 376, 423, 810
BbvI GCAGC 2 cut(s) 220, 298
BccI CCATC 3 cut(s) 116, 794, 1132
BciT130I CCWGG 3 cut(s) 666, 780, 966
BciVI GTATCC 1 cut(s) 1083
BcoDI GTCTC 4 cut(s) 263, 394, 422, 762
BfaI CTAG 3 cut(s) 192, 293, 1094
BfmI CTRYAG 1 cut(s) 64
BfuI GTATCC 1 cut(s) 1083
BisI GCNGC 4 cut(s) 85, 234, 287, 462
BlsI GCNGC 4 cut(s) 86, 235, 288, 463
Bme1390I CCNGG 3 cut(s) 666, 780, 966
BmgBI CACGTC 1 cut(s) 880
BmrFI CCNGG 3 cut(s) 666, 780, 966
BmsI GCATC 2 cut(s) 25, 253
BpmI CTGGAG 1 cut(s) 987
BpuEI CTTGAG 2 cut(s) 437, 801
BsaJI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 800
Bse1I ACTGG 2 cut(s) 553, 815
BseBI CCWGG 3 cut(s) 666, 780, 966
BseDI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
BseGI GGATG 2 cut(s) 40, 840
BseLI CCNNNNNNNGG 2 cut(s) 141, 800
BseNI ACTGG 2 cut(s) 553, 815
BseRI GAGGAG 1 cut(s) 1031
BseSI GKGCMC 3 cut(s) 376, 810, 891
BseXI GCAGC 2 cut(s) 220, 298
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 2 cut(s) 474, 1019
BshFI GGCC 2 cut(s) 358, 1116
BsiHKAI GWGCWC 3 cut(s) 376, 423, 810
BsiSI CCGG 1 cut(s) 81
BslI CCNNNNNNNGG 2 cut(s) 141, 800
BsmAI GTCTC 4 cut(s) 263, 394, 422, 762
BsnI GGCC 2 cut(s) 358, 1116
Bsp1286I GDGCHC 4 cut(s) 376, 423, 810, 891
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 3 cut(s) 703, 747, 985
Bsp19I CCATGG 3 cut(s) 267, 334, 1111
BspACI CCGC 3 cut(s) 84, 158, 461
BspANI GGCC 2 cut(s) 358, 1116
BspQI GCTCTTC 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 2 cut(s) 553, 815
BssECI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
BssMI GATC 3 cut(s) 703, 747, 985
BssNAI GTATAC 1 cut(s) 873
BssT1I CCWWGG 3 cut(s) 267, 334, 1111
Bst1107I GTATAC 1 cut(s) 873
Bst2UI CCWGG 3 cut(s) 666, 780, 966
Bst4CI ACNGT 5 cut(s) 68, 437, 868, 900, 1081
Bst6I CTCTTC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 697
BstC8I GCNNGC 3 cut(s) 20, 284, 413
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 3 cut(s) 267, 334, 1111
BstF5I GGATG 2 cut(s) 40, 840
BstKTI GATC 3 cut(s) 706, 750, 988
BstMAI GTCTC 4 cut(s) 263, 394, 422, 762
BstMBI GATC 3 cut(s) 703, 747, 985
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 3 cut(s) 666, 780, 966
BstSCI CCNGG 3 cut(s) 664, 778, 964
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 3 cut(s) 376, 810, 891
BstV1I GCAGC 2 cut(s) 220, 298
BstZ17I GTATAC 1 cut(s) 873
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 2 cut(s) 358, 1116
BtgI CCRYGG 3 cut(s) 267, 334, 1111
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 40, 840
BtsIMutI CAGTG 3 cut(s) 283, 560, 808
Cac8I GCNNGC 3 cut(s) 20, 284, 413
Csp6I GTAC 4 cut(s) 72, 698, 860, 936
CspCI CAANNNNNGTGG 4 cut(s) 159, 194, 811, 846
CviQI GTAC 4 cut(s) 72, 698, 860, 936
DdeI CTNAG 1 cut(s) 254
DpnI GATC 3 cut(s) 705, 749, 987
DpnII GATC 3 cut(s) 703, 747, 985
EaeI YGGCCR 1 cut(s) 1114
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 267, 334, 1111
Eco57I CTGAAG 1 cut(s) 938
EcoRII CCWGG 3 cut(s) 664, 778, 964
EcoT14I CCWWGG 3 cut(s) 267, 334, 1111
ErhI CCWWGG 3 cut(s) 267, 334, 1111
FblI GTMKAC 1 cut(s) 872
Fnu4HI GCNGC 4 cut(s) 85, 234, 287, 462
FokI GGATG 2 cut(s) 47, 847
Fsp4HI GCNGC 4 cut(s) 85, 234, 287, 462
FspBI CTAG 3 cut(s) 192, 293, 1094
GluI GCNGC 4 cut(s) 85, 234, 287, 462
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 2 cut(s) 358, 1116
HapII CCGG 1 cut(s) 81
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 4 cut(s) 20, 173, 504, 673
HinfI GANTC 7 cut(s) 167, 210, 329, 350, 621, 814, 1097
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 5 cut(s) 74, 374, 634, 808, 873
Hpy188I TCNGA 3 cut(s) 27, 136, 1067
Hpy188III TCNNGA 7 cut(s) 192, 260, 539, 797, 818, 1042, 1129
Hpy8I GTNNAC 5 cut(s) 74, 374, 634, 808, 873
HpyAV CCTTC 6 cut(s) 560, 590, 596, 962, 971, 1114
HpyCH4III ACNGT 5 cut(s) 68, 437, 868, 900, 1081
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 8 cut(s) 374, 455, 558, 577, 609, 662, 808, 1036
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 879
Kzo9I GATC 3 cut(s) 703, 747, 985
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 123, 1018
Lsp1109I GCAGC 2 cut(s) 220, 298
LweI GCATC 2 cut(s) 25, 253
MaeI CTAG 3 cut(s) 192, 293, 1094
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 5 cut(s) 160, 223, 740, 916, 1003
MalI GATC 3 cut(s) 705, 749, 987
MboI GATC 3 cut(s) 703, 747, 985
MboII GAAGA 7 cut(s) 68, 118, 503, 605, 970, 1144, 1147
MhlI GDGCHC 4 cut(s) 376, 423, 810, 891
MlsI TGGCCA 1 cut(s) 1116
MluCI AATT 5 cut(s) 28, 214, 481, 582, 1143
MluNI TGGCCA 1 cut(s) 1116
MlyI GAGTC 2 cut(s) 323, 808
MmeI TCCRAC 1 cut(s) 1045
MnlI CCTC 8 cut(s) 107, 115, 140, 188, 463, 804, 1009, 1061
Mox20I TGGCCA 1 cut(s) 1116
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 1 cut(s) 1116
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
Msp20I TGGCCA 1 cut(s) 1116
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 666, 780, 966
MvaI CCWGG 3 cut(s) 666, 780, 966
MwoI GCNNNNNNNGC 1 cut(s) 461
NcoI CCATGG 3 cut(s) 267, 334, 1111
NdeII GATC 3 cut(s) 703, 747, 985
NmuCI GTSAC 3 cut(s) 223, 740, 916
PciSI GCTCTTC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 5 cut(s) 167, 210, 350, 621, 1097
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 86, 235, 288, 463
PleI GAGTC 2 cut(s) 323, 808
PpsI GAGTC 2 cut(s) 323, 808
Psp6I CCWGG 3 cut(s) 664, 778, 964
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 3 cut(s) 664, 778, 964
RsaI GTAC 4 cut(s) 73, 699, 861, 937
RsaNI GTAC 4 cut(s) 72, 698, 860, 936
RseI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 85, 234, 287, 462
Sau3AI GATC 3 cut(s) 703, 747, 985
SchI GAGTC 2 cut(s) 323, 808
ScrFI CCNGG 3 cut(s) 666, 780, 966
SduI GDGCHC 4 cut(s) 376, 423, 810, 891
SfaNI GCATC 2 cut(s) 25, 253
SfcI CTRYAG 1 cut(s) 64
SmiMI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SmlI CTYRAG 2 cut(s) 416, 816
SmoI CTYRAG 2 cut(s) 416, 816
Sse9I AATT 5 cut(s) 28, 214, 481, 582, 1143
SsiI CCGC 3 cut(s) 84, 158, 461
SspMI CTAG 3 cut(s) 192, 293, 1094
StyD4I CCNGG 3 cut(s) 664, 778, 964
StyI CCWWGG 3 cut(s) 267, 334, 1111
TaaI ACNGT 5 cut(s) 68, 437, 868, 900, 1081
TaiI ACGT 1 cut(s) 882
TaqI TCGA 1 cut(s) 261
TaqII GACCGA 1 cut(s) 935
TasI AATT 5 cut(s) 28, 214, 481, 582, 1143
TatI WGTACW 1 cut(s) 697
TauI GCSGC 2 cut(s) 87, 464
TfiI GAWTC 5 cut(s) 167, 210, 350, 621, 1097
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 3 cut(s) 283, 560, 815
TseFI GTSAC 3 cut(s) 223, 740, 916
TseI GCWGC 2 cut(s) 233, 286
Tsp45I GTSAC 3 cut(s) 223, 740, 916
TspDTI ATGAA 7 cut(s) 69, 432, 474, 606, 683, 1017, 1089
TspGWI ACGGA 3 cut(s) 179, 488, 1028
TspRI CASTG 3 cut(s) 283, 560, 815
VneI GTGCAC 2 cut(s) 372, 806
XapI RAATTY 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 3 cut(s) 192, 293, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.