MD15G1365600.v1.1

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011866
Physical Location & Seq
Reverse (-)
44307101 .. 44318225
11125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1365600.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGAGAACGTAATGAGCAAGCTTCGGAATTTGGATGCTTACCCAAAAATCAACGAAGATTTCTACAGTCGTACACTCTCCGGCGGCGTTATCACTCTCGCTTCCTCCATCGTCATGCTCCTGCTCTTCCTCTCCGAGCTTCAGTTATATCTCCATGCGGTAACGGAATCAAAGCTTGTGGTCGATACTTCTAGAGGCGAAACCTTACGAATCAATTTTGATGTAACTTTTGCTGCCCTCCCTTGTTCTATACTAAGTCTCGATGCCATGGACATCAGTGGCGAGCAGCACCTAGATGTAAAACATGACATTATCAAAAAAAGATTAGACTCCCATGGAAATGTGATAGAATCAAGGCCAGATGTTATTGGTGCACCCAAGATTGACAAGCCCTTACAGAGACATGGTGGAAGGCTTGAGCATAATGAGACATACTGTGGTTCCTGTTATGGTGCAGAAGCGGCTGATGAGGATTGTTGTAATTCATGTGAAGATGTCCGTGAAGCTTACCGAAAGAAAGGTTGGGCAATATCAAATCCAGATTTGATTGACCAGTGTAAAAGAGAAGGCTTTCTGCAAAGAATTAAAGATGAAGAAGGTGAAGGGTGCAATATTTATGGTTTTTTGGAAGTCAACAAGGTAGCTGGGAACTTCCATTTTGCACCTGGGAAAAGCTTTCAACAATCAAATGTTCATGTGCATGATCTGCTGGCATTTCAAAAGGATAGTTTCAATATTAGTCACACAATCAACAGATTGTCTTTTGGAGACTATTTCCCTGGTGTTGTAAATCCTCTTGATGGTGTGCACTGGACACAAGACAACCCAAGTGGGATGTATCAATACTTTATCAAGGTTGTACCCACTGTGTATACAGATGTGAGTGGGCACACTATCCAGTCAAATCAGTTCTCTGTAACTGAACATTATAGGGGTACAGAAATGGGCAACCTTCAGTTCCTTCCTGGAGTGTTTTTCTTCTATGATCTTTCTCCCATAAAGGTAACTTTCACAGAGGAGCATATTTCATTCTTGCACTTCCTGACCAACGTTTGTGCCATAGTAGGAGGTGTCTTTACAGTTTCGGGAATATTAGATTCGTTTATATACCATGGTCAGAAGGCAATCAAGAAGAAGATGGAGATCGGCAAATTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

387

Amino Acids

43.38

Weight (kDa)

5.72

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 8 - 97 2.6e-31 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 149 - 366 6.3e-89 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36050 AT1G36050
fragaria_vesca FvH4_5g37810 FvH4_5g37810 FvH4_5g37810
malus_domestica MD08G1180300.v1.1 MD15G1365600.v1.1
prunus_persica Prupe.1G513700_v2.0.a1
pyrus_communis pycom08g15430 pycom15g32800
rosa_chinensis RchiOBHm_Chr7g0239691
rosa_laevigata RLG00000000741
rosa_multiflora Rmu_sc0000063.1_g000019
rosa_roxburghii Rroxscaffold_3G00220830
rosa_samantha Rh7AG482900 Rh7BG455600 Rh7CG500300 Rh7DG468500
rosa_wichuraiana Rw7G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 872
AciI CCGC 3 cut(s) 84, 158, 461
AclI AACGTT 1 cut(s) 1050
AcsI RAATTY 2 cut(s) 28, 1151
AcuI CTGAAG 2 cut(s) 125, 938
AfaI GTAC 3 cut(s) 73, 861, 937
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 3 cut(s) 680, 719, 733
AjnI CCWGG 3 cut(s) 664, 778, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
AluI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
Alw21I GWGCWC 2 cut(s) 376, 810
Alw26I GTCTC 4 cut(s) 263, 394, 422, 762
Alw44I GTGCAC 2 cut(s) 372, 806
AoxI GGCC 1 cut(s) 356
ApaLI GTGCAC 2 cut(s) 372, 806
ApeKI GCWGC 2 cut(s) 233, 286
ApoI RAATTY 2 cut(s) 28, 1151
Asp700I GAANNNNTTC 1 cut(s) 570
AsuHPI GGTGA 1 cut(s) 611
BaeGI GKGCMC 3 cut(s) 376, 810, 891
Bbv12I GWGCWC 2 cut(s) 376, 810
BbvI GCAGC 2 cut(s) 220, 298
BccI CCATC 3 cut(s) 116, 794, 1132
BciT130I CCWGG 3 cut(s) 666, 780, 966
BcoDI GTCTC 4 cut(s) 263, 394, 422, 762
BfaI CTAG 2 cut(s) 192, 293
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 4 cut(s) 85, 234, 287, 462
BlsI GCNGC 4 cut(s) 86, 235, 288, 463
Bme1390I CCNGG 3 cut(s) 666, 780, 966
BmiI GGNNCC 1 cut(s) 442
BmrFI CCNGG 3 cut(s) 666, 780, 966
BmsI GCATC 2 cut(s) 25, 253
BpmI CTGGAG 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 437
BsaBI GATNNNNATC 1 cut(s) 1142
BsaJI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 3 cut(s) 553, 815, 898
Bse8I GATNNNNATC 1 cut(s) 1142
BseBI CCWGG 3 cut(s) 666, 780, 966
BseDI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
BseGI GGATG 2 cut(s) 40, 840
BseJI GATNNNNATC 1 cut(s) 1142
BseLI CCNNNNNNNGG 1 cut(s) 800
BseNI ACTGG 3 cut(s) 553, 815, 898
BseRI GAGGAG 1 cut(s) 1031
BseSI GKGCMC 3 cut(s) 376, 810, 891
BseXI GCAGC 2 cut(s) 220, 298
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 474
BshFI GGCC 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 376, 810
BsiSI CCGG 1 cut(s) 81
BslI CCNNNNNNNGG 1 cut(s) 800
BsmAI GTCTC 4 cut(s) 263, 394, 422, 762
BsnI GGCC 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 376, 810, 891
Bsp143I GATC 3 cut(s) 703, 985, 1143
Bsp19I CCATGG 3 cut(s) 267, 334, 1111
BspACI CCGC 3 cut(s) 84, 158, 461
BspANI GGCC 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 442
BspQI GCTCTTC 1 cut(s) 131
BsrI ACTGG 3 cut(s) 553, 815, 898
BssECI CCNNGG 5 cut(s) 267, 334, 665, 778, 1111
BssMI GATC 3 cut(s) 703, 985, 1143
BssNAI GTATAC 1 cut(s) 873
BssT1I CCWWGG 3 cut(s) 267, 334, 1111
Bst1107I GTATAC 1 cut(s) 873
Bst2UI CCWGG 3 cut(s) 666, 780, 966
Bst4CI ACNGT 4 cut(s) 68, 437, 868, 1081
Bst6I CTCTTC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 706
BstC8I GCNNGC 3 cut(s) 20, 284, 711
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 3 cut(s) 267, 334, 1111
BstF5I GGATG 2 cut(s) 40, 840
BstKTI GATC 3 cut(s) 706, 988, 1146
BstMAI GTCTC 4 cut(s) 263, 394, 422, 762
BstMBI GATC 3 cut(s) 703, 985, 1143
BstMWI GCNNNNNNNGC 2 cut(s) 461, 706
BstNI CCWGG 3 cut(s) 666, 780, 966
BstSCI CCNGG 3 cut(s) 664, 778, 964
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 3 cut(s) 376, 810, 891
BstV1I GCAGC 2 cut(s) 220, 298
BstZ17I GTATAC 1 cut(s) 873
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 3 cut(s) 267, 334, 1111
BtsCI GGATG 2 cut(s) 40, 840
BtsIMutI CAGTG 4 cut(s) 283, 560, 808, 864
Cac8I GCNNGC 3 cut(s) 20, 284, 711
Csp6I GTAC 3 cut(s) 72, 860, 936
CspCI CAANNNNNGTGG 4 cut(s) 159, 194, 811, 846
CviQI GTAC 3 cut(s) 72, 860, 936
DdeI CTNAG 1 cut(s) 254
DpnI GATC 3 cut(s) 705, 987, 1145
DpnII GATC 3 cut(s) 703, 985, 1143
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 267, 334, 1111
Eco57I CTGAAG 2 cut(s) 125, 938
EcoRII CCWGG 3 cut(s) 664, 778, 964
EcoT14I CCWWGG 3 cut(s) 267, 334, 1111
ErhI CCWWGG 3 cut(s) 267, 334, 1111
FblI GTMKAC 1 cut(s) 872
Fnu4HI GCNGC 4 cut(s) 85, 234, 287, 462
FokI GGATG 2 cut(s) 47, 847
Fsp4HI GCNGC 4 cut(s) 85, 234, 287, 462
FspBI CTAG 2 cut(s) 192, 293
GluI GCNGC 4 cut(s) 85, 234, 287, 462
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 1 cut(s) 358
HapII CCGG 1 cut(s) 81
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 4 cut(s) 20, 173, 504, 673
HinfI GANTC 5 cut(s) 167, 210, 329, 350, 1097
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 5 cut(s) 74, 374, 634, 808, 873
Hpy188I TCNGA 3 cut(s) 27, 136, 1119
Hpy188III TCNNGA 7 cut(s) 192, 260, 539, 797, 1042, 1086, 1129
Hpy8I GTNNAC 5 cut(s) 74, 374, 634, 808, 873
HpyAV CCTTC 7 cut(s) 405, 560, 590, 596, 962, 971, 1114
HpyCH4III ACNGT 4 cut(s) 68, 437, 868, 1081
HpyCH4IV ACGT 2 cut(s) 9, 1050
HpyCH4V TGCA 8 cut(s) 374, 455, 577, 609, 662, 700, 808, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 706
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 2 cut(s) 9, 1050
Kzo9I GATC 3 cut(s) 703, 985, 1143
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 123, 1018
Lsp1109I GCAGC 2 cut(s) 220, 298
LweI GCATC 2 cut(s) 25, 253
MaeI CTAG 2 cut(s) 192, 293
MaeII ACGT 2 cut(s) 9, 1050
MaeIII GTNAC 5 cut(s) 160, 223, 740, 916, 1003
MalI GATC 3 cut(s) 705, 987, 1145
MboI GATC 3 cut(s) 703, 985, 1143
MboII GAAGA 7 cut(s) 68, 118, 503, 605, 970, 1144, 1147
MhlI GDGCHC 3 cut(s) 376, 810, 891
MluCI AATT 5 cut(s) 28, 214, 481, 582, 1151
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 8 cut(s) 115, 140, 188, 248, 463, 804, 1009, 1061
MroXI GAANNNNTTC 1 cut(s) 570
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 666, 780, 966
MvaI CCWGG 3 cut(s) 666, 780, 966
MwoI GCNNNNNNNGC 2 cut(s) 461, 706
NcoI CCATGG 3 cut(s) 267, 334, 1111
NdeII GATC 3 cut(s) 703, 985, 1143
NlaIV GGNNCC 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 4 cut(s) 167, 210, 350, 1097
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 86, 235, 288, 463
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Psp1406I AACGTT 1 cut(s) 1050
Psp6I CCWGG 3 cut(s) 664, 778, 964
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 3 cut(s) 664, 778, 964
PspN4I GGNNCC 1 cut(s) 442
RsaI GTAC 3 cut(s) 73, 861, 937
RsaNI GTAC 3 cut(s) 72, 860, 936
RseI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 85, 234, 287, 462
Sau3AI GATC 3 cut(s) 703, 985, 1143
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 3 cut(s) 666, 780, 966
SduI GDGCHC 3 cut(s) 376, 810, 891
SfaNI GCATC 2 cut(s) 25, 253
SfcI CTRYAG 1 cut(s) 64
SmiMI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 5 cut(s) 28, 214, 481, 582, 1151
SsiI CCGC 3 cut(s) 84, 158, 461
SspI AATATT 3 cut(s) 613, 736, 1092
SspMI CTAG 2 cut(s) 192, 293
StyD4I CCNGG 3 cut(s) 664, 778, 964
StyI CCWWGG 3 cut(s) 267, 334, 1111
TaaI ACNGT 4 cut(s) 68, 437, 868, 1081
TaiI ACGT 2 cut(s) 12, 1053
TaqI TCGA 2 cut(s) 183, 261
TasI AATT 5 cut(s) 28, 214, 481, 582, 1151
TauI GCSGC 2 cut(s) 87, 464
TfiI GAWTC 4 cut(s) 167, 210, 350, 1097
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 4 cut(s) 283, 560, 815, 871
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 2 cut(s) 233, 286
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 4 cut(s) 474, 606, 683, 1017
TspGWI ACGGA 2 cut(s) 179, 488
TspRI CASTG 4 cut(s) 283, 560, 815, 871
VneI GTGCAC 2 cut(s) 372, 806
XapI RAATTY 2 cut(s) 28, 1151
XbaI TCTAGA 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 2 cut(s) 192, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.