Rw7G040420

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011866
Physical Location & Seq
Forward (+)
62922720 .. 62929145
6426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040420.1

Sequence Viewer

Length: 1236 bp
ATGGAGAATATGATGAGCCGCCTCCGCAATTTGGACGCGTACCCCAAAATCAACGAAGATTTCTACAGCCGTACCCTCTCCGGCGGCGTCATCACACTCGTCTCCTCCATCGTTATGCTCCTGCTCTTCCTCTCCGAGCTCCAGTTATATCTACATGCAGTGACGGAAACGAAGCTTGTGGTGGATACTTCAAGAGGAGAAACCCTGCGTATCAATTTCGATGTAACCTTTGCTGCGCTTCCTTGTTCTATACTCAGTCTTGATGCCATGGACATTAGTGGAGAGCAGCACCTTGATGTAAAACATGACATTATCAAGAAAAGAATAGACTCCAATGGAAATGTTATAGAATCAAGGCCGGATGGAATTGGTGCCCCCAAGATTGACAAGCCTTTACAGAGACATGGTGGCAGGCTTGAGCGCAATGAGACATACTGTGGTTCTTGTTATGGGGCAGAAGCGAATGATGAGGATTGTTGTAATAACTGTGAAGAGGTCCGTGAAGCTTACCGAAAGAAAGGTTGGGCCATTTCAAATCCAGATTTGATTGACCAGTGCAAAAGAGAAGGGTTTTTGCAAAAGATTAAAGATGAAGAAGGTGAAGGATGCAATATATATGGGTTCTTGGAAGTCAATAAGGTGGCCGGGAACTTTCATTTTGCACCTGGGAAAAGCTTTCAACAATCAAATGTTCATGTACACGATTTGCTGGCATTTCAAAAGGATAGTTTTAATATAAGTCACACAATCAACAGATTGTCTTTTGGAGACTATTTCCCTGGTGTTGTCAATCCTCTTGATGGGGTGCACTGGACGCAGGACAACCCCAGTGGGATGTATCAATATTTTATCAAGGTTGTTCCTACTGTTTACACAGATGTCAGGGGGCACACTATCCAGTCAAATCAGTTCTCTGTCACTGAACATTATAGGGGTACAGAAATAGGCAACCTTCAGTTTCTTCCTGGAATATTTTTCTTCTATGACCTTTCCCCGATAAAGGTAACTTTTACGGAGGAGCATATTTCTTTCTTACACTTCCTGACAAATGTGTGTGCCATTGTTGGAGGTGTTTTTACCGTGTCTGGAATATTGGATTCGTTTATATACCATGGTCAGAAGGCCTGGCAACTGGGCAGTCACGGGCGGGTTGCGGGCAGACTTAGGGTTAAGATAACTGGTTATAACGGGTTGAGTCAAATGGATTCACGGGTTACCCGTTGGAACCCCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

411

Amino Acids

46.34

Weight (kDa)

6.01

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 8 - 97 1e-31 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 149 - 366 2e-88 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36050 AT1G36050
fragaria_vesca FvH4_5g37810 FvH4_5g37810 FvH4_5g37810
malus_domestica MD08G1180300.v1.1 MD15G1365600.v1.1
prunus_persica Prupe.1G513700_v2.0.a1
pyrus_communis pycom08g15430 pycom15g32800
rosa_chinensis RchiOBHm_Chr7g0239691
rosa_laevigata RLG00000000741
rosa_multiflora Rmu_sc0000063.1_g000019
rosa_roxburghii Rroxscaffold_3G00220830
rosa_samantha Rh7AG482900 Rh7BG455600 Rh7CG500300 Rh7DG468500
rosa_wichuraiana Rw7G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 38
AciI CCGC 5 cut(s) 19, 25, 84, 1147, 1154
AcoI YGGCCR 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 938
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 4 cut(s) 41, 73, 699, 937
AfiI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 4 cut(s) 192, 534, 680, 719
AjnI CCWGG 4 cut(s) 664, 778, 964, 1124
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 4 cut(s) 139, 175, 506, 675
AluI AGCT 4 cut(s) 139, 175, 506, 675
Alw21I GWGCWC 2 cut(s) 141, 810
Alw26I GTCTC 4 cut(s) 106, 394, 422, 762
Alw44I GTGCAC 1 cut(s) 806
AoxI GGCC 4 cut(s) 356, 525, 642, 1122
ApaLI GTGCAC 1 cut(s) 806
ApeKI GCWGC 2 cut(s) 233, 286
AspLEI GCGC 2 cut(s) 238, 423
AspS9I GGNCC 2 cut(s) 496, 525
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 611
AvaII GGWCC 1 cut(s) 496
BaeGI GKGCMC 3 cut(s) 376, 810, 891
BaeI ACNNNNGTAYC 2 cut(s) 55, 88
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 2 cut(s) 141, 810
BbvI GCAGC 2 cut(s) 220, 298
BccI CCATC 3 cut(s) 116, 356, 794
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 4 cut(s) 666, 780, 966, 1126
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 4 cut(s) 106, 394, 422, 762
BfmI CTRYAG 1 cut(s) 64
BfuI GTATCC 1 cut(s) 178
BisI GCNGC 4 cut(s) 19, 85, 234, 287
BlsI GCNGC 4 cut(s) 20, 86, 235, 288
Bme1390I CCNGG 5 cut(s) 646, 666, 780, 966, 1126
Bme18I GGWCC 1 cut(s) 496
BmgT120I GGNCC 2 cut(s) 496, 525
BmiI GGNNCC 2 cut(s) 373, 1226
BmrFI CCNGG 5 cut(s) 646, 666, 780, 966, 1126
BmrI ACTGGG 2 cut(s) 822, 1142
BmsI GCATC 2 cut(s) 253, 596
BmuI ACTGGG 2 cut(s) 822, 1142
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 4 cut(s) 267, 665, 778, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 800, 1000
Bse1I ACTGG 7 cut(s) 142, 553, 815, 828, 898, 1137, 1183
Bse3DI GCAATG 1 cut(s) 430
BseBI CCWGG 4 cut(s) 666, 780, 966, 1126
BseDI CCNNGG 4 cut(s) 267, 665, 778, 1111
BseGI GGATG 3 cut(s) 367, 611, 840
BseLI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 7 cut(s) 142, 553, 815, 828, 898, 1137, 1183
BseRI GAGGAG 3 cut(s) 94, 210, 1031
BseSI GKGCMC 3 cut(s) 376, 810, 891
BseXI GCAGC 2 cut(s) 220, 298
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 4 cut(s) 358, 527, 644, 1124
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 141, 810
BsiSI CCGG 3 cut(s) 81, 359, 645
BslI CCNNNNNNNGG 3 cut(s) 31, 800, 1000
BsmAI GTCTC 4 cut(s) 106, 394, 422, 762
BsmBI CGTCTC 1 cut(s) 106
BsnI GGCC 4 cut(s) 358, 527, 644, 1124
Bsp1286I GDGCHC 4 cut(s) 141, 376, 810, 891
Bsp1407I TGTACA 1 cut(s) 697
Bsp19I CCATGG 2 cut(s) 267, 1111
BspACI CCGC 5 cut(s) 19, 25, 84, 1147, 1154
BspANI GGCC 4 cut(s) 358, 527, 644, 1124
BspCNI CTCAG 1 cut(s) 267
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 373, 1226
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 430
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 7 cut(s) 142, 553, 815, 828, 898, 1137, 1183
BssECI CCNNGG 4 cut(s) 267, 665, 778, 1111
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 2 cut(s) 267, 1111
Bst2UI CCWGG 4 cut(s) 666, 780, 966, 1126
Bst4CI ACNGT 4 cut(s) 437, 488, 868, 1081
Bst6I CTCTTC 2 cut(s) 131, 486
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 697
BstC8I GCNNGC 3 cut(s) 413, 711, 1156
BstDEI CTNAG 2 cut(s) 254, 1163
BstDSI CCRYGG 2 cut(s) 267, 1111
BstEII GGTNACC 1 cut(s) 1213
BstF5I GGATG 3 cut(s) 367, 611, 840
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 2 cut(s) 238, 423
BstMAI GTCTC 4 cut(s) 106, 394, 422, 762
BstMWI GCNNNNNNNGC 2 cut(s) 24, 814
BstNI CCWGG 4 cut(s) 666, 780, 966, 1126
BstNSI RCATGY 1 cut(s) 158
BstPI GGTNACC 1 cut(s) 1213
BstSCI CCNGG 5 cut(s) 644, 664, 778, 964, 1124
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 3 cut(s) 376, 810, 891
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 220, 298
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 4 cut(s) 358, 527, 644, 1124
BtgI CCRYGG 2 cut(s) 267, 1111
BtsCI GGATG 3 cut(s) 367, 611, 840
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 5 cut(s) 165, 560, 808, 835, 918
Cac8I GCNNGC 3 cut(s) 413, 711, 1156
CfoI GCGC 2 cut(s) 238, 423
Cfr13I GGNCC 2 cut(s) 496, 525
CseI GACGC 3 cut(s) 44, 76, 823
Csp6I GTAC 4 cut(s) 40, 72, 698, 936
CspCI CAANNNNNGTGG 2 cut(s) 811, 846
CviAII CATG 6 cut(s) 155, 268, 305, 404, 695, 1112
CviQI GTAC 4 cut(s) 40, 72, 698, 936
DdeI CTNAG 2 cut(s) 254, 1163
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 2 cut(s) 131, 486
EarI CTCTTC 2 cut(s) 131, 486
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 267, 1111
Eco147I AGGCCT 1 cut(s) 1124
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 938
Eco91I GGTNACC 1 cut(s) 1213
EcoICRI GAGCTC 1 cut(s) 139
EcoO65I GGTNACC 1 cut(s) 1213
EcoRII CCWGG 4 cut(s) 664, 778, 964, 1124
EcoT14I CCWWGG 2 cut(s) 267, 1111
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 267, 1111
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 6 cut(s) 158, 271, 308, 407, 698, 1115
FatI CATG 6 cut(s) 154, 267, 304, 403, 694, 1111
FauI CCCGC 2 cut(s) 1140, 1147
Fnu4HI GCNGC 4 cut(s) 19, 85, 234, 287
FokI GGATG 3 cut(s) 374, 618, 847
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 4 cut(s) 19, 85, 234, 287
GlaI GCGC 2 cut(s) 237, 422
GluI GCNGC 4 cut(s) 19, 85, 234, 287
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 4 cut(s) 358, 527, 644, 1124
HapII CCGG 3 cut(s) 81, 359, 645
HgaI GACGC 3 cut(s) 44, 76, 823
HhaI GCGC 2 cut(s) 238, 423
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 6 cut(s) 158, 271, 308, 407, 698, 1115
Hin6I GCGC 2 cut(s) 236, 421
HinP1I GCGC 2 cut(s) 236, 421
HindIII AAGCTT 3 cut(s) 173, 504, 673
HinfI GANTC 5 cut(s) 329, 350, 1097, 1195, 1205
HpaII CCGG 3 cut(s) 81, 359, 645
HphI GGTGA 1 cut(s) 611
Hpy166II GTNNAC 3 cut(s) 700, 808, 871
Hpy188I TCNGA 2 cut(s) 136, 1119
Hpy188III TCNNGA 7 cut(s) 192, 260, 316, 539, 797, 1042, 1086
Hpy8I GTNNAC 3 cut(s) 700, 808, 871
HpyAV CCTTC 5 cut(s) 560, 590, 596, 962, 1114
HpyCH4III ACNGT 4 cut(s) 437, 488, 868, 1081
HpyCH4V TGCA 6 cut(s) 158, 558, 577, 609, 662, 808
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 814
HpyF3I CTNAG 2 cut(s) 254, 1163
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 6 cut(s) 158, 271, 308, 407, 698, 1115
HspAI GCGC 2 cut(s) 236, 421
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 3 cut(s) 123, 144, 1018
Lsp1109I GCAGC 2 cut(s) 220, 298
LweI GCATC 2 cut(s) 253, 596
MaeIII GTNAC 7 cut(s) 160, 223, 740, 916, 1003, 1139, 1213
MboII GAAGA 6 cut(s) 68, 118, 503, 605, 953, 970
MhlI GDGCHC 4 cut(s) 141, 376, 810, 891
MluCI AATT 3 cut(s) 28, 214, 366
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 2 cut(s) 323, 1204
MmeI TCCRAC 2 cut(s) 1045, 1202
MseI TTAA 3 cut(s) 585, 732, 1170
MslI CAYNNNNRTG 2 cut(s) 113, 294
MspI CCGG 3 cut(s) 81, 359, 645
MspR9I CCNGG 5 cut(s) 646, 666, 780, 966, 1126
MvaI CCWGG 4 cut(s) 666, 780, 966, 1126
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 24, 814
NciI CCSGG 1 cut(s) 646
NcoI CCATGG 2 cut(s) 267, 1111
NlaIII CATG 6 cut(s) 158, 271, 308, 407, 698, 1115
NlaIV GGNNCC 2 cut(s) 373, 1226
NmuCI GTSAC 4 cut(s) 160, 740, 916, 1139
NspI RCATGY 1 cut(s) 158
PceI AGGCCT 1 cut(s) 1124
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 1216
PfeI GAWTC 3 cut(s) 350, 1097, 1205
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 20, 86, 235, 288
PleI GAGTC 2 cut(s) 323, 1203
PpsI GAGTC 2 cut(s) 323, 1203
PsiI TTATAA 1 cut(s) 1185
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 4 cut(s) 664, 778, 964, 1124
PspEI GGTNACC 1 cut(s) 1213
PspGI CCWGG 4 cut(s) 664, 778, 964, 1124
PspN4I GGNNCC 2 cut(s) 373, 1226
PspPI GGNCC 2 cut(s) 496, 525
RsaI GTAC 4 cut(s) 41, 73, 699, 937
RsaNI GTAC 4 cut(s) 40, 72, 698, 936
RseI CAYNNNNRTG 2 cut(s) 113, 294
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 3 cut(s) 585, 732, 1170
SatI GCNGC 4 cut(s) 19, 85, 234, 287
Sau96I GGNCC 2 cut(s) 496, 525
SchI GAGTC 2 cut(s) 323, 1204
ScrFI CCNGG 5 cut(s) 646, 666, 780, 966, 1126
SduI GDGCHC 4 cut(s) 141, 376, 810, 891
SfaNI GCATC 2 cut(s) 253, 596
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 1 cut(s) 496
SmiMI CAYNNNNRTG 2 cut(s) 113, 294
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 3 cut(s) 28, 214, 366
SseBI AGGCCT 1 cut(s) 1124
SsiI CCGC 5 cut(s) 19, 25, 84, 1147, 1154
SspI AATATT 3 cut(s) 845, 972, 1092
SstI GAGCTC 1 cut(s) 141
StuI AGGCCT 1 cut(s) 1124
StyD4I CCNGG 5 cut(s) 644, 664, 778, 964, 1124
StyI CCWWGG 2 cut(s) 267, 1111
TaaI ACNGT 4 cut(s) 437, 488, 868, 1081
TaqI TCGA 1 cut(s) 219
TasI AATT 3 cut(s) 28, 214, 366
TatI WGTACW 1 cut(s) 697
TauI GCSGC 2 cut(s) 21, 87
TfiI GAWTC 3 cut(s) 350, 1097, 1205
Tru1I TTAA 3 cut(s) 585, 732, 1170
Tru9I TTAA 3 cut(s) 585, 732, 1170
TscAI CASTG 5 cut(s) 165, 560, 815, 835, 925
TseFI GTSAC 4 cut(s) 160, 740, 916, 1139
TseI GCWGC 2 cut(s) 233, 286
Tsp45I GTSAC 4 cut(s) 160, 740, 916, 1139
TspDTI ATGAA 3 cut(s) 606, 644, 683
TspGWI ACGGA 3 cut(s) 179, 488, 1028
TspRI CASTG 5 cut(s) 165, 560, 815, 835, 925
VneI GTGCAC 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 496
XceI RCATGY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.