pycom08g15430

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011866
Physical Location & Seq
Reverse (-)
15070121 .. 15075359
5239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g15430.12

Sequence Viewer

Length: 1158 bp
ATGGAGAATATGATGAGCAAGCTTCGGAATTTGGATGCTTACCCAAAAATCAATGAAGATTTCTACAGTCGTACACTCTCAGGCGGCGTTATCACTCTCGCTTCCTCCATCGTCATGCTCCTGCTCTTCCTCTCCGAGCTTCGGTTATATCTCCATGCGGTAACGGAATCAAAGCTTGTGGTAGATACTTCTAGAGGCGAAACCTTACGAATCAATTTTGATGTGACTTTTGCTGCCTTGCCTTGTTCTATACTAAGTCTCGATGCCATGGACATCAGTGGCGAGCAGCACCTAGATGTAAAACATGACATTATCAAAAAAAGATTAGACTCCCATGGAAATGTGATAGAATCAAGGCCAGATGTTATTGGTGCACCCAAGATTGATAAGCCCTTACAGAGACATGGTGGCAGGCTTGAGCACAATGAGACGTACTGTGGTTCATGTTATGGTGCAGAAGCGGCTGACGAGGATTGTTGTAATTCATGTGAAGATGTCCGTGAAGCTTACCGAAAGAAAGGTTGGGCAATATCAAATCCCGATTTGATTGACCAGTGCAAAAGAGAAGGCTTTCTGCAAAGAATTAAAGATGAAGAAGGGGAAGGGTGCAATATATATGGATTTTTGGAAGTCAACAAGGTAGCTGGGAACTTCCATTTTGCACCTGGGAAAAGCTTTCAACAATCAAATGTTCATGTACATGATCTGTTTGCATTTCAAAAGGATAGTTTCAATATAAGTCACACGATCAACAGATTGTCTTTTGGAGACTATTTCCCTGGTGTTGTAAATCCTCTTGATGGTGTGCACTGGACTCAAGACAACCCAAGTGGGATGTATCAATACTTTATCAAGGTTGTGCCTACTGTGTATACAGACGTGAGTGGGCACACTATACAGTCAAATCAGTTCTCTGTGACTGAACATTATAGGGGTACAGAAATGGGCAACCTTCAGTTCCTTCCTGGAGTGTTTTTCTTCTATCGATCTTTCTCCTATAAAGGTAACTTTCACGGAGGAGCATATTTCATTCCTGCACTTCCTGACAAATGTTTGTGCCATAGTCGGAGGTGTTTTTACAGTTTCGGGGATACTAGATTCCTTTATATACCATGGCCAGAAGGCAATCAAGAAGAAGATGGAAATTGGCAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

386

Amino Acids

43.74

Weight (kDa)

5.67

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011866)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36050 AT1G36050
fragaria_vesca FvH4_5g37810 FvH4_5g37810 FvH4_5g37810
malus_domestica MD08G1180300.v1.1 MD15G1365600.v1.1
prunus_persica Prupe.1G513700_v2.0.a1
pyrus_communis pycom08g15430 pycom15g32800
rosa_chinensis RchiOBHm_Chr7g0239691
rosa_laevigata RLG00000000741
rosa_multiflora Rmu_sc0000063.1_g000019
rosa_roxburghii Rroxscaffold_3G00220830
rosa_samantha Rh7AG482900 Rh7BG455600 Rh7CG500300 Rh7DG468500
rosa_wichuraiana Rw7G040420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 872
AciI CCGC 3 cut(s) 84, 158, 461
AcoI YGGCCR 1 cut(s) 1115
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 938
AfaI GTAC 4 cut(s) 73, 434, 699, 937
AfiI CCNNNNNNNGG 2 cut(s) 141, 800
AgsI TTSAA 3 cut(s) 680, 719, 733
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 3 cut(s) 664, 778, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 505, 537
AluBI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
AluI AGCT 6 cut(s) 22, 139, 175, 506, 644, 675
Alw21I GWGCWC 3 cut(s) 376, 423, 810
Alw26I GTCTC 4 cut(s) 263, 394, 422, 762
Alw44I GTGCAC 2 cut(s) 372, 806
AoxI GGCC 2 cut(s) 356, 1115
ApaLI GTGCAC 2 cut(s) 372, 806
ApeKI GCWGC 2 cut(s) 233, 286
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 570
BaeGI GKGCMC 3 cut(s) 376, 810, 891
BalI TGGCCA 1 cut(s) 1117
Bbv12I GWGCWC 3 cut(s) 376, 423, 810
BbvI GCAGC 2 cut(s) 220, 298
BccI CCATC 3 cut(s) 116, 794, 1133
BciT130I CCWGG 3 cut(s) 666, 780, 966
BciVI GTATCC 1 cut(s) 1084
BcoDI GTCTC 4 cut(s) 263, 394, 422, 762
BfaI CTAG 3 cut(s) 192, 293, 1095
BfmI CTRYAG 1 cut(s) 64
BfuI GTATCC 1 cut(s) 1084
BisI GCNGC 4 cut(s) 85, 234, 287, 462
BlsI GCNGC 4 cut(s) 86, 235, 288, 463
Bme1390I CCNGG 3 cut(s) 666, 780, 966
BmgBI CACGTC 1 cut(s) 880
BmrFI CCNGG 3 cut(s) 666, 780, 966
BmsI GCATC 2 cut(s) 25, 253
BpmI CTGGAG 1 cut(s) 987
BpuEI CTTGAG 2 cut(s) 437, 801
Bsa29I ATCGAT 1 cut(s) 985
BsaJI CCNNGG 5 cut(s) 267, 334, 665, 778, 1112
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 800
Bse1I ACTGG 2 cut(s) 553, 815
BseBI CCWGG 3 cut(s) 666, 780, 966
BseCI ATCGAT 1 cut(s) 985
BseDI CCNNGG 5 cut(s) 267, 334, 665, 778, 1112
BseGI GGATG 2 cut(s) 40, 840
BseLI CCNNNNNNNGG 2 cut(s) 141, 800
BseMII CTCAG 1 cut(s) 93
BseNI ACTGG 2 cut(s) 553, 815
BseRI GAGGAG 1 cut(s) 1032
BseSI GKGCMC 3 cut(s) 376, 810, 891
BseXI GCAGC 2 cut(s) 220, 298
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 2 cut(s) 474, 1020
BshFI GGCC 2 cut(s) 358, 1117
BshVI ATCGAT 1 cut(s) 985
BsiHKAI GWGCWC 3 cut(s) 376, 423, 810
BslI CCNNNNNNNGG 2 cut(s) 141, 800
BsmAI GTCTC 4 cut(s) 263, 394, 422, 762
BsmBI CGTCTC 1 cut(s) 422
BsnI GGCC 2 cut(s) 358, 1117
Bsp1286I GDGCHC 4 cut(s) 376, 423, 810, 891
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 3 cut(s) 703, 747, 986
Bsp19I CCATGG 3 cut(s) 267, 334, 1112
BspACI CCGC 3 cut(s) 84, 158, 461
BspANI GGCC 2 cut(s) 358, 1117
BspCNI CTCAG 1 cut(s) 92
BspDI ATCGAT 1 cut(s) 985
BspQI GCTCTTC 1 cut(s) 131
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 2 cut(s) 553, 815
BssECI CCNNGG 5 cut(s) 267, 334, 665, 778, 1112
BssMI GATC 3 cut(s) 703, 747, 986
BssNAI GTATAC 1 cut(s) 873
BssT1I CCWWGG 3 cut(s) 267, 334, 1112
Bst1107I GTATAC 1 cut(s) 873
Bst2UI CCWGG 3 cut(s) 666, 780, 966
Bst4CI ACNGT 5 cut(s) 68, 437, 868, 900, 1082
Bst6I CTCTTC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 697
BstC8I GCNNGC 3 cut(s) 20, 284, 413
BstDEI CTNAG 2 cut(s) 79, 254
BstDSI CCRYGG 3 cut(s) 267, 334, 1112
BstF5I GGATG 2 cut(s) 40, 840
BstKTI GATC 3 cut(s) 706, 750, 989
BstMAI GTCTC 4 cut(s) 263, 394, 422, 762
BstMBI GATC 3 cut(s) 703, 747, 986
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNI CCWGG 3 cut(s) 666, 780, 966
BstSCI CCNGG 3 cut(s) 664, 778, 964
BstSFI CTRYAG 1 cut(s) 64
BstSLI GKGCMC 3 cut(s) 376, 810, 891
BstV1I GCAGC 2 cut(s) 220, 298
BstZ17I GTATAC 1 cut(s) 873
Bsu15I ATCGAT 1 cut(s) 985
BsuI GTATCC 1 cut(s) 1084
BsuRI GGCC 2 cut(s) 358, 1117
BsuTUI ATCGAT 1 cut(s) 985
BtgI CCRYGG 3 cut(s) 267, 334, 1112
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 40, 840
BtsIMutI CAGTG 3 cut(s) 283, 560, 808
Cac8I GCNNGC 3 cut(s) 20, 284, 413
ClaI ATCGAT 1 cut(s) 985
Csp6I GTAC 4 cut(s) 72, 433, 698, 936
CspCI CAANNNNNGTGG 4 cut(s) 159, 194, 811, 846
CviQI GTAC 4 cut(s) 72, 433, 698, 936
DdeI CTNAG 2 cut(s) 79, 254
DpnI GATC 3 cut(s) 705, 749, 988
DpnII GATC 3 cut(s) 703, 747, 986
EaeI YGGCCR 1 cut(s) 1115
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 267, 334, 1112
Eco57I CTGAAG 1 cut(s) 938
EcoRII CCWGG 3 cut(s) 664, 778, 964
EcoT14I CCWWGG 3 cut(s) 267, 334, 1112
ErhI CCWWGG 3 cut(s) 267, 334, 1112
Esp3I CGTCTC 1 cut(s) 422
FblI GTMKAC 1 cut(s) 872
Fnu4HI GCNGC 4 cut(s) 85, 234, 287, 462
FokI GGATG 2 cut(s) 47, 847
Fsp4HI GCNGC 4 cut(s) 85, 234, 287, 462
FspBI CTAG 3 cut(s) 192, 293, 1095
GluI GCNGC 4 cut(s) 85, 234, 287, 462
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 2 cut(s) 358, 1117
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 4 cut(s) 20, 173, 504, 673
HinfI GANTC 6 cut(s) 167, 210, 329, 350, 814, 1098
Hpy166II GTNNAC 5 cut(s) 74, 374, 634, 808, 873
Hpy188I TCNGA 3 cut(s) 27, 136, 1068
Hpy188III TCNNGA 7 cut(s) 192, 260, 539, 797, 818, 1043, 1130
Hpy8I GTNNAC 5 cut(s) 74, 374, 634, 808, 873
HpyAV CCTTC 6 cut(s) 560, 590, 596, 962, 971, 1115
HpyCH4III ACNGT 5 cut(s) 68, 437, 868, 900, 1082
HpyCH4IV ACGT 2 cut(s) 431, 879
HpyCH4V TGCA 9 cut(s) 374, 455, 558, 577, 609, 662, 713, 808, 1037
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 2 cut(s) 79, 254
HpySE526I ACGT 2 cut(s) 431, 879
Kzo9I GATC 3 cut(s) 703, 747, 986
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 123, 1019
Lsp1109I GCAGC 2 cut(s) 220, 298
LweI GCATC 2 cut(s) 25, 253
MaeI CTAG 3 cut(s) 192, 293, 1095
MaeII ACGT 2 cut(s) 431, 879
MaeIII GTNAC 5 cut(s) 160, 223, 740, 916, 1004
MalI GATC 3 cut(s) 705, 749, 988
MboI GATC 3 cut(s) 703, 747, 986
MboII GAAGA 7 cut(s) 68, 118, 503, 605, 970, 1145, 1148
MhlI GDGCHC 4 cut(s) 376, 423, 810, 891
MlsI TGGCCA 1 cut(s) 1117
MluCI AATT 5 cut(s) 28, 214, 481, 582, 1144
MluNI TGGCCA 1 cut(s) 1117
MlyI GAGTC 2 cut(s) 323, 808
MmeI TCCRAC 1 cut(s) 1046
MnlI CCTC 7 cut(s) 115, 140, 188, 463, 804, 1010, 1062
Mox20I TGGCCA 1 cut(s) 1117
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 1 cut(s) 1117
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
Msp20I TGGCCA 1 cut(s) 1117
MspR9I CCNGG 3 cut(s) 666, 780, 966
MvaI CCWGG 3 cut(s) 666, 780, 966
MwoI GCNNNNNNNGC 1 cut(s) 461
NcoI CCATGG 3 cut(s) 267, 334, 1112
NdeII GATC 3 cut(s) 703, 747, 986
NmuCI GTSAC 3 cut(s) 223, 740, 916
PciSI GCTCTTC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 4 cut(s) 167, 210, 350, 1098
PfoI TCCNGGA 1 cut(s) 964
PkrI GCNGC 4 cut(s) 86, 235, 288, 463
PleI GAGTC 2 cut(s) 323, 808
PpsI GAGTC 2 cut(s) 323, 808
Psp6I CCWGG 3 cut(s) 664, 778, 964
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 3 cut(s) 664, 778, 964
RsaI GTAC 4 cut(s) 73, 434, 699, 937
RsaNI GTAC 4 cut(s) 72, 433, 698, 936
RseI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 85, 234, 287, 462
Sau3AI GATC 3 cut(s) 703, 747, 986
SchI GAGTC 2 cut(s) 323, 808
ScrFI CCNGG 3 cut(s) 666, 780, 966
SduI GDGCHC 4 cut(s) 376, 423, 810, 891
SfaNI GCATC 2 cut(s) 25, 253
SfcI CTRYAG 1 cut(s) 64
SmiMI CAYNNNNRTG 4 cut(s) 113, 294, 339, 699
SmlI CTYRAG 2 cut(s) 416, 816
SmoI CTYRAG 2 cut(s) 416, 816
Sse9I AATT 5 cut(s) 28, 214, 481, 582, 1144
SsiI CCGC 3 cut(s) 84, 158, 461
SspMI CTAG 3 cut(s) 192, 293, 1095
StyD4I CCNGG 3 cut(s) 664, 778, 964
StyI CCWWGG 3 cut(s) 267, 334, 1112
TaaI ACNGT 5 cut(s) 68, 437, 868, 900, 1082
TaiI ACGT 2 cut(s) 434, 882
TaqI TCGA 2 cut(s) 261, 985
TasI AATT 5 cut(s) 28, 214, 481, 582, 1144
TatI WGTACW 1 cut(s) 697
TauI GCSGC 2 cut(s) 87, 464
TfiI GAWTC 4 cut(s) 167, 210, 350, 1098
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 3 cut(s) 283, 560, 815
TseFI GTSAC 3 cut(s) 223, 740, 916
TseI GCWGC 2 cut(s) 233, 286
Tsp45I GTSAC 3 cut(s) 223, 740, 916
TspDTI ATGAA 6 cut(s) 69, 432, 474, 606, 683, 1018
TspGWI ACGGA 3 cut(s) 179, 488, 1029
TspRI CASTG 3 cut(s) 283, 560, 815
VneI GTGCAC 2 cut(s) 372, 806
XapI RAATTY 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 872
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 3 cut(s) 192, 293, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.